The present disclosure relates to a new process for construction of controlled structures on the surface of modified digital barcoded magnetic beads (BMB) with higher capacity, higher fluorescence intensity, wider dynamic range, and improved sensitivity in clinical applications.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
C08G 59/14 - Polycondensats modifiés par post-traitement chimique
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
2.
PROCESS FOR HIGH PERFORMANCE BARCODED MAGNETIC BEADS
The present disclosure relates to a new process for construction of controlled structures on the surface of modified digital barcoded magnetic beads (BMB) with higher capacity, higher fluorescence intensity, wider dynamic range, and improved sensitivity in clinical applications.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
3.
Method for Dynamic Range Expansion for Multiplex Assays
Techniques for conducting an assay for multiple analytes in a sample wherein the presence/absence of each analyte in said sample is indicated by a light signal are provided. An example method includes determining the presence of an analyte by: providing a light to the sample, wherein a light signal is emitted from the sample associated with the presence of individual analytes, capturing a plurality of images by one or more photodetectors, each including the plurality of analytes, at a plurality of durations, and for respective analytes of the plurality of analytes, determining a range of integration durations associated with a light signal associated with the presence of the analyte, identifying an image of the plurality of images, captured at a duration within the range of integration durations, and analyzing the image to determine the presence of the analyte.
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
4.
METHOD FOR DYNAMIC RANGE EXPANSION FOR MULTIPLEX ASSAYS
Techniques for conducting an assay for multiple analytes in a sample wherein the presence/absence of each analyte in said sample is indicated by a light signal are provided. An example method includes determining the presence of an analyte by: providing a light to the sample, wherein a light signal is emitted from the sample associated with the presence of individual analytes, capturing a plurality of images by one or more photodetectors, each including the plurality of analytes, at a plurality of durations, and for respective analytes of the plurality of analytes, determining a range of integration durations associated with a light signal associated with the presence of the analyte, identifying an image of the plurality of images, captured at a duration within the range of integration durations, and analyzing the image to determine the presence of the analyte.
An integrated magnetic microbead processing apparatus includes a microplate having magnetic microbeads in microwells. A bottom of the microplate includes cavities between the microwells. A heating/cooling plate supports a base of the microplate and has a plurality of holes. A vertically movable magnet support plate includes a plurality of magnetic pins, each of which can protrude through one hole in the plurality of holes of the heating plate and into the cavity of the microplate. The magnetic pins are height adjustable. A shaker is operably connected to the microplate and is configured to shake the microplate, the heating/cooling plate, the magnetic support plate.
B01F 31/22 - Mélange du contenu de récipients indépendants, p. ex. de tubes à essai avec des moyens de support se déplaçant dans un plan horizontal, p. ex. en décrivant un trajet orbital pour déplacer les récipients autour d'un axe qui coupe l'axe du récipient selon un certain angle
B01D 35/06 - Filtres utilisant l'électricité ou le magnétisme
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
6.
Integrated Magnetic Bead Assay Processing Method and Apparatus
An integrated magnetic microbead processing apparatus includes a microplate having magnetic microbeads in microwells. A bottom of the microplate includes cavities between the microwells. A heating/cooling plate supports a base of the microplate and has a plurality of holes. A vertically movable magnet support plate includes a plurality of magnetic pins, each of which can protrude through one hole in the plurality of holes of the heating plate and into the cavity of the microplate. The magnetic pins are height adjustable. A shaker is operably connected to the microplate and is configured to shake the microplate, the heating/cooling plate, the magnetic support plate.
B03C 1/06 - Séparation magnétique agissant directement sur la substance à séparer ayant des supports pour le matériau traité, en forme de plateaux, ou constitués par des tables avec des aimants se déplaçant pendant l'opération
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
Produits et services
Medical diagnostic reagents and assays for testing body fluids for microorganisms Instrument and apparatus systems for medical diagnostic uses consisting of flow cytometers, hematology analyzers, sample preparation device, and related recorded and downloadable data management software sold as a unit
A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone.
A Light Transmitted Assay Beads or digital magnetic microbead having a digitally coded structure that is partially transmissive and opaque to light. When hundreds or thousands of LITAB are settled down to the bottom of a micro well in a microplate or a planar surface, the barcode can be decoded by image processed accurately and reliable. Microplate is a standard bioassay format. Therefore, a large number of targets in a sample can be analyzed in one single microwell. The image decoding algorithms comprise of four main processes enhancement of image segmentation of beads extraction of barcode slits, and decoding of barcodes. The bead image is taken from the bottom of an optically clear microplate, and barcode pattern can be decoded by image software. Therefore, the whole bead bioassay experiment can be performed in the microplate without taking the beads out.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G06K 7/10 - Méthodes ou dispositions pour la lecture de supports d'enregistrement par radiation électromagnétique, p. ex. lecture optiqueMéthodes ou dispositions pour la lecture de supports d'enregistrement par radiation corpusculaire
[The present invention relates to substrates for biological testing produced from photo-curable epoxy compositions which further include carboxyl-containing monomers such as acrylic acid, 2-carboxyethyl acrylic acid, 4-vinylbenzoic acid, or 3 -Acrylamido-3 -methyl- 1- butanoic acid, or glycidyl methacrylate, etc. The photo-curable compositions may be used to cast films or fabricate beads, magnetic beads, or magnetic beads containing nickel barcodes. The resulting various kinds of films, beads, magnetic beads, or magnetic beads containing nickel barcodes may find use in clinical or biological applications.
G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur
The present invention relates to substrates for biological testing produced from photo-curable epoxy compositions which further include carboxyl-containing monomers such as acrylic acid, 2-carboxyethyl acrylic acid, 4-vinylbenzoic acid, or 3-Acrylamido-3-methyl-1-butanoic acid, or glycidyl methacrylate, etc. The photo-curable compositions may be used to cast films or fabricate beads, magnetic beads, or magnetic beads containing nickel barcodes. The resulting various kinds of films, beads, magnetic beads, or magnetic beads containing nickel barcodes may find use in clinical or biological applications.
C08F 8/00 - Modification chimique par post-traitement
C08F 2/50 - Polymérisation amorcée par énergie ondulatoire ou par rayonnement corpusculaire par la lumière ultraviolette ou visible avec des agents sensibilisants
C08F 220/18 - Esters des alcools ou des phénols monohydriques des phénols ou des alcools contenant plusieurs atomes de carbone avec l'acide acrylique ou l'acide méthacrylique
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G03F 7/26 - Traitement des matériaux photosensiblesAppareillages à cet effet
12.
Apparatus and method for digital magnetic beads analysis
A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone. Alternative method for barcode determination is based on barcode pattern image processes. Microbeads can also be settled down to the bottom of the well in a microplate, so the barcode can be decoded by image processed directly. Therefore, multiple-analyte tests can be performed in one single well. Microplate is a standard format; each plate can have 96, 384, or 1536 patient samples. The bead image is taken with a conventional microscope and optical detector from the bottom of an optically clear microplate, and barcode pattern can be decoded by image software. Therefore, the whole bead experiment can be performed in the microplate without taking the beads out.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
C40B 60/12 - Appareils spécialement adaptés à une utilisation en chimie combinatoire ou avec des bibliothèques pour cribler des bibliothèques
A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
