Abstract

The present disclosure relates to a new process for construction of controlled structures on the surface of modified digital barcoded magnetic beads (BMB) with higher capacity, higher fluorescence intensity, wider dynamic range, and improved sensitivity in clinical applications.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• C08G 59/14 - Polycondensates modified by chemical after-treatment
• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

The present disclosure relates to a new process for construction of controlled structures on the surface of modified digital barcoded magnetic beads (BMB) with higher capacity, higher fluorescence intensity, wider dynamic range, and improved sensitivity in clinical applications.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

Techniques for conducting an assay for multiple analytes in a sample wherein the presence/absence of each analyte in said sample is indicated by a light signal are provided. An example method includes determining the presence of an analyte by: providing a light to the sample, wherein a light signal is emitted from the sample associated with the presence of individual analytes, capturing a plurality of images by one or more photodetectors, each including the plurality of analytes, at a plurality of durations, and for respective analytes of the plurality of analytes, determining a range of integration durations associated with a light signal associated with the presence of the analyte, identifying an image of the plurality of images, captured at a duration within the range of integration durations, and analyzing the image to determine the presence of the analyte.

IPC Classes  ?

• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

Techniques for conducting an assay for multiple analytes in a sample wherein the presence/absence of each analyte in said sample is indicated by a light signal are provided. An example method includes determining the presence of an analyte by: providing a light to the sample, wherein a light signal is emitted from the sample associated with the presence of individual analytes, capturing a plurality of images by one or more photodetectors, each including the plurality of analytes, at a plurality of durations, and for respective analytes of the plurality of analytes, determining a range of integration durations associated with a light signal associated with the presence of the analyte, identifying an image of the plurality of images, captured at a duration within the range of integration durations, and analyzing the image to determine the presence of the analyte.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• C12Q 1/6825 - Nucleic acid detection involving sensors

Abstract

An integrated magnetic microbead processing apparatus includes a microplate having magnetic microbeads in microwells. A bottom of the microplate includes cavities between the microwells. A heating/cooling plate supports a base of the microplate and has a plurality of holes. A vertically movable magnet support plate includes a plurality of magnetic pins, each of which can protrude through one hole in the plurality of holes of the heating plate and into the cavity of the microplate. The magnetic pins are height adjustable. A shaker is operably connected to the microplate and is configured to shake the microplate, the heating/cooling plate, the magnetic support plate.

IPC Classes  ?

• B01F 31/22 - Mixing the contents of independent containers, e.g. test tubes with supporting means moving in a horizontal plane, e.g. describing an orbital path for moving the containers about an axis which intersects the receptacle axis at an angle
• B01D 35/06 - Filters making use of electricity or magnetism
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

An integrated magnetic microbead processing apparatus includes a microplate having magnetic microbeads in microwells. A bottom of the microplate includes cavities between the microwells. A heating/cooling plate supports a base of the microplate and has a plurality of holes. A vertically movable magnet support plate includes a plurality of magnetic pins, each of which can protrude through one hole in the plurality of holes of the heating plate and into the cavity of the microplate. The magnetic pins are height adjustable. A shaker is operably connected to the microplate and is configured to shake the microplate, the heating/cooling plate, the magnetic support plate.

IPC Classes  ?

• B03C 1/06 - Magnetic separation acting directly on the substance being separated with the material carriers in the form of trays or with tables with magnets moving during operation

Goods & Services

Medical diagnostic reagents and assays for testing body fluids for microorganisms Instrument and apparatus systems for medical diagnostic uses consisting of flow cytometers, hematology analyzers, sample preparation device, and related recorded and downloadable data management software sold as a unit

Abstract

A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• G01N 15/06 - Investigating concentration of particle suspensions

Abstract

A Light Transmitted Assay Beads or digital magnetic microbead having a digitally coded structure that is partially transmissive and opaque to light. When hundreds or thousands of LITAB are settled down to the bottom of a micro well in a microplate or a planar surface, the barcode can be decoded by image processed accurately and reliable. Microplate is a standard bioassay format. Therefore, a large number of targets in a sample can be analyzed in one single microwell. The image decoding algorithms comprise of four main processes enhancement of image segmentation of beads extraction of barcode slits, and decoding of barcodes. The bead image is taken from the bottom of an optically clear microplate, and barcode pattern can be decoded by image software. Therefore, the whole bead bioassay experiment can be performed in the microplate without taking the beads out.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G06K 7/10 - Methods or arrangements for sensing record carriers by electromagnetic radiation, e.g. optical sensingMethods or arrangements for sensing record carriers by corpuscular radiation

Abstract

[The present invention relates to substrates for biological testing produced from photo-curable epoxy compositions which further include carboxyl-containing monomers such as acrylic acid, 2-carboxyethyl acrylic acid, 4-vinylbenzoic acid, or 3 -Acrylamido-3 -methyl- 1- butanoic acid, or glycidyl methacrylate, etc. The photo-curable compositions may be used to cast films or fabricate beads, magnetic beads, or magnetic beads containing nickel barcodes. The resulting various kinds of films, beads, magnetic beads, or magnetic beads containing nickel barcodes may find use in clinical or biological applications.

IPC Classes  ?

• G01N 33/52 - Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper

Abstract

The present invention relates to substrates for biological testing produced from photo-curable epoxy compositions which further include carboxyl-containing monomers such as acrylic acid, 2-carboxyethyl acrylic acid, 4-vinylbenzoic acid, or 3-Acrylamido-3-methyl-1-butanoic acid, or glycidyl methacrylate, etc. The photo-curable compositions may be used to cast films or fabricate beads, magnetic beads, or magnetic beads containing nickel barcodes. The resulting various kinds of films, beads, magnetic beads, or magnetic beads containing nickel barcodes may find use in clinical or biological applications.

IPC Classes  ?

• C08F 8/00 - Chemical modification by after-treatment
• C08F 2/50 - Polymerisation initiated by wave energy or particle radiation by ultraviolet or visible light with sensitising agents
• G01N 33/545 - Synthetic resin
• C08F 220/18 - Esters of monohydric alcohols or phenols of phenols or of alcohols containing two or more carbon atoms with acrylic or methacrylic acids
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G03F 7/26 - Processing photosensitive materialsApparatus therefor

Abstract

A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone. Alternative method for barcode determination is based on barcode pattern image processes. Microbeads can also be settled down to the bottom of the well in a microplate, so the barcode can be decoded by image processed directly. Therefore, multiple-analyte tests can be performed in one single well. Microplate is a standard format; each plate can have 96, 384, or 1536 patient samples. The bead image is taken with a conventional microscope and optical detector from the bottom of an optically clear microplate, and barcode pattern can be decoded by image software. Therefore, the whole bead experiment can be performed in the microplate without taking the beads out.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• G01N 21/00 - Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
• C40B 60/12 - Apparatus specially adapted for use in combinatorial chemistry or with libraries for screening libraries

Abstract

A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical