Abrégé

The present invention provides a method for determining a background count rate in liquid scintillation counting. The method comprises measuring external standard spectra of a sample, determining, from the external standard spectra, a triple to double coincidence ratio and a quench parameter, determining, based on the triple to double coincidence ratio and the quench parameter, a background reference parameter, and determining, based on the background reference parameter, the background count rate from a background reference curve.

Classes IPC  ?

• G01T 1/204 - Mesure de l'intensité de radiation avec des détecteurs à scintillation le détecteur étant un liquide
• G01T 1/20 - Mesure de l'intensité de radiation avec des détecteurs à scintillation

Produits et services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical preparations for analysis in laboratories for use in life science research and radiation measurement; Chemical substances for analyses in laboratories for use in life science research and radiation measurement; Diagnostic reagents, other than for medical laboratory use; Fluorescent dye for scientific or research use; Reagents for laboratory purposes, other than for medical or veterinary purposes; Diagnostic chemicals, other than for medical or veterinary use; Chemical compositions and materials for use in science; Reagents for scientific purposes in life science research, radiation measurement and nuclear medicine; Chemicals for use in scientific research, other than medical or veterinary; Reference standards being chemicals for use in science; Indicators being chemicals for scientific use

Produits et services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical preparations for analysis in laboratories for use in life science research and radiation measurement; Chemical substances for analyses in laboratories for use in life science research and radiation measurement; Diagnostic reagents, other than for medical laboratory use; Fluorescent dye for scientific or research use; Reagents for laboratory purposes, other than for medical or veterinary purposes; Diagnostic chemicals, other than for medical or veterinary use; Chemical compositions and materials for use in science; Reagents for scientific purposes in life science research, radiation measurement and nuclear medicine; Chemicals for use in scientific research, other than medical or veterinary; Reference standards being chemicals for use in science; Indicators being chemicals for scientific use; all being for life science research, radiation measurement and nuclear medicine

Produits et services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical substances for analysis in laboratories; Chemical substances for analyses in laboratories, other than for medical or veterinary purposes; Diagnostic reagents, other than for medical laboratory use; Fluorescent chemicals; Reagents for laboratory purposes [other than for medical or veterinary purposes]; Diagnostic chemicals [other than for medical or veterinary use]; Chemicals for use in analysis [other than medical and veterinary]; Chemical compositions and materials for use in science; Reagents for scientific purposes; Chemicals for use in scientific research [other than medical or veterinary]; Reference standards [chemicals] for use in science; Indicators [chemicals] for scientific use.

Produits et services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical substances for analysis in laboratories; Chemical substances for analyses in laboratories, other than for medical or veterinary purposes; Diagnostic reagents, other than for medical laboratory use; Fluorescent chemicals; Reagents for laboratory purposes [other than for medical or veterinary purposes]; Diagnostic chemicals [other than for medical or veterinary use]; Chemicals for use in analysis [other than medical and veterinary]; Chemical compositions and materials for use in science; Reagents for scientific purposes; Chemicals for use in scientific research [other than medical or veterinary]; Reference standards [chemicals] for use in science; Indicators [chemicals] for scientific use.

Abrégé

The present invention provides a method for determining a background count rate in liquid scintillation counting. The method comprises measuring an external standard spectrum (201, 202) of a sample, determining, from the external standard spectrum, an external standard count rate within an energy window (203), determining, based on the external standard count rate within the energy window, a background reference parameter, and determining, based on the background reference parameter, the background count rate from a background reference curve (301).

Classes IPC  ?

• G01T 1/178 - Dispositions de circuits non adaptés à un type particulier de détecteur pour la mesure d'une activité spécifique en présence d'autres substances radioactives, p. ex. des substances naturelles, présentes dans l'air ou dans des liquides tels que l'eau de pluie
• G01T 1/204 - Mesure de l'intensité de radiation avec des détecteurs à scintillation le détecteur étant un liquide

Produits et services

Reagents for research purposes; chemical reagents for laboratory use, other than for medical or veterinary use; chemical substances for analysis in laboratories; chemical substances for analyses in laboratories, other than for medical or veterinary purposes; diagnostic reagents, other than for medical laboratory use; fluorescent chemicals; reagents for laboratory purposes [other than for medical or veterinary purposes]; diagnostic chemicals [other than for medical or veterinary use]; chemicals for use in analysis [other than medical and veterinary]; chemical compositions and materials for use in science; reagents for scientific purposes; chemicals for use in scientific research [other than medical or veterinary]; reference standards [chemicals] for use in science; indicators [chemicals] for scientific use. Scientific research and laboratory apparatus, educational apparatus and simulators; laboratory apparatus and instruments; microplate readers; scientific apparatus and instruments; liquid scintillation counters; photometric analyzers [other than for medical use]; measuring, detecting and monitoring instruments, indicators and controllers; luminescence measuring devices; fluorescence analyzers; measuring, counting, alignment and calibrating instruments; optical detection apparatus; luminescence scanners; gamma counters; measuring apparatus and instruments; radiation measuring apparatus; fluorometers; software; application software; operating software; all being for life science research, radiation measurement and nuclear medicine.

Produits et services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical preparations for analysis in laboratories for use in life science research and radiation measurement; Chemical substances for analyses in laboratories for use in life science research and radiation measurement; Diagnostic reagents, other than for medical laboratory use; Fluorescent dye for scientific or research use; Reagents for laboratory purposes, other than for medical or veterinary purposes; Diagnostic chemicals, other than for medical or veterinary use; Chemical compositions and materials for use in science; Reagents for scientific purposes in life science research, radiation measurement and nuclear medicine; Chemicals for use in scientific research, other than medical or veterinary; Reference standards being chemicals for use in science; Indicators being chemicals for scientific use, all being for life science research, radiation measurement and nuclear medicine Laboratory apparatus and instruments, namely, vials, cuvettes, beakers, filters, swabs and microplates; Microplate readers; Liquid scintillation counters; Photometric analyzers other than for medical use; Luminescence measuring devices; Fluorescence analyzers; Luminescence scanners; Gamma counters; Radiation measuring apparatus; Fluorometers; Downloadable computer software for performing measurement data calculations derived from laboratory, research or scientific instruments; Embedded Recorded software for the operation of microplate readers, liquid scintillation counters, luminescence measuring devices, fluorescence analyzers, luminescence scanners, gamma counters, radiation measuring apparatus and fluorometers; Recorded or downloadable software for the operation of microplate readers, liquid scintillation counters, luminescence measuring devices, fluorescence analyzers, luminescence scanners, gamma counters, radiation measuring apparatus and fluorometers; all being for life science research, radiation measurement and nuclear medicine

Produits et services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical substances for analysis in laboratories; Chemical substances for analyses in laboratories, other than for medical or veterinary purposes; Diagnostic reagents, other than for medical laboratory use; Fluorescent chemicals; Reagents for laboratory purposes [other than for medical or veterinary purposes]; Diagnostic chemicals [other than for medical or veterinary use]; Chemicals for use in analysis [other than medical and veterinary]; Chemical compositions and materials for use in science; Reagents for scientific purposes; Chemicals for use in scientific research [other than medical or veterinary]; Reference standards [chemicals] for use in science; Indicators [chemicals] for scientific use. Scientific research and laboratory apparatus, educational apparatus and simulators; Laboratory apparatus and instruments; Microplate readers; Scientific apparatus and instruments; Liquid scintillation counters; Photometric analyzers [other than for medical use]; Measuring, detecting and monitoring instruments, indicators and controllers; Luminescence measuring devices; Fluorescence analyzers; Measuring, counting, alignment and calibrating instruments; Detection apparatus; Luminescence scanners; Gamma counters; Measuring apparatus and instruments; Radiation measuring apparatus; Fluorometers; Software; Application software; Operating software; all being for life science research, radiation measurement and nuclear medicine.

Abrégé

A homogenous bioassay including i) a first group containing a short lifetime fluorescent acceptor, and ii) a second group containing a quencher, with the first and second groups linked by at least a first linkage. The bioassay measures the acceptor's fluorescence increase resulting from cleavage of the first linkage and also includes iii) a third group containing a donor for energy transfer to the acceptor, where the donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. A conformational or terminal epitope is created on the first group through linkage cleavage, and the third group includes a binder with affinity for this epitope. The acceptor's fluorescence is caused by exciting the donor. Also disclosed are bioassay kits for this method.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

A homogenous bioassay including a first group including an acceptor which is a short lifetime fluorescent compound capable of energy transfer, and a second group including a quencher which is capable of energy transfer from an acceptor. The increase or decrease of the acceptor's fluorescence resulting from lengthening or shortening of the distance between the acceptor and quencher is measured. The bioassay also includes a third group including a donor for energy transfer to the acceptor, which donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. The first group includes a tag, the third group includes a binder having a high affinity for binding to the tag. Fluorescence of the acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide

Abrégé

This invention relates to a luminescence energy transfer based homogeneous bioassay wherein measurement of phosphorylated or dephosphorylated peptide or protein is carried out. The bioassay comprises a) a first group labelled with an energy donor; wherein the first group is a particulate comprised of a polymer or an inorganic crystallic structure, and the first group is i) a long-lifetime luminescent lanthanide label if the particulate is a polymer, or ii) an up-conversion luminescent lanthanide label or down-conversion luminescent lanthanide label if the particulate is an inorganic crystallic structure. The first group is able to transfer fluorescence energy to an acceptor of a second group, and the first group i) is coated with a trivalent metal ion binder through passive adsorption, achelate structure or a polymeric structure, or ii) has trivalent metal ions incorporated into the crystallic structure. The second group is labell ed with the energy acceptor, wherein the acceptor is a short-lifetime fluorescent label, and the second group comprises a peptide or protein with at least one phosphorylatable amino acid within 10 nm from said acceptor. After a sample and reagents of the bioassay, the reagents including the first and second groups, and trivalent metalions if not incorporated in the second group, have been brought into contact with each other, the first group is excited and resulting emission of the second group, the emission relating to energy transfer from donor to acceptor due to phosphorylated peptides and/or proteins of the second group being attached to the trivalent metal ions bound to or incorporated into the first group, is measured. The present invention also relates to kits for bioassays according to the invention.

Classes IPC  ?

• G01N 33/542 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec inhibition stérique ou modification du signal, p. ex. extinction de fluorescence
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/553 - Support métallique ou recouvert d'un métal

Abrégé

This invention relates to a homogenous bioassay for use in measurement of biological activity, its modulation or analyte concentration of a sample, said bioassay comprising i) a first group comprising an acceptor, which acceptor is a short lifetime fluorescent compound capable of energy transfer, and ii) a second group comprising a quencher, which quencher is capable of energy transfer from an acceptor, and the first and second group are linked by at least a first linkage, and an increase, of fluorescence of said acceptor due to the decrease, of energy transfer from said acceptor to said quencher resulting from cleavage of said first linkage, is measured. Characteristic for the bioassay is that it comprises iii) a further third group comprising a donor for energy transfer to said acceptor, which donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound; and a conformational or terminal epitope is created on said first group through said cleavage of the linkage, and said third group comprises a binder with affinity for binding to said conformational or terminal epitope; and the fluorescence of said acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor. This invention also relates to kits for homogenous bioassays according to the method of the invention.

Classes IPC  ?

• G01N 33/542 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec inhibition stérique ou modification du signal, p. ex. extinction de fluorescence

Abrégé

A method to correct measurement error in a resonance energy-transfer assay, including exciting anti-Stokes photoluminescent donors with at least one wavelength of light which is greater than an emission wavelength of acceptor molecules; measuring emission at the acceptor's emission wavelength and which differs from the donor's emission wavelength in at least two different time windows; a first time window within the time window defined by the excitation light pulse and a second non-overlapping time window which follows the first time window; and correcting the emission signal, which includes signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission signal measured in the second time window.

Classes IPC  ?

• G01N 21/64 - FluorescencePhosphorescence

Abrégé

This invention relates to a homogenous bioassay for use in measurement of biological activity, its modulation or analyte concentration of a sample, said bioassay comprising a first group comprising an acceptor, which acceptor is a short lifetime fluorescent compound capable of energy transfer, and a second group comprising a quencher, which quencher is capable of energy transfer from an acceptor. The increase or decrease, respectively, of fluorescence of said acceptor due to the decrease or increase, respectively, of energy transfer from said acceptor to said quencher resulting from lengthening or shortening, respectively, of the distance between said acceptor and quencher is measured. Characteristic for the invention is that the bioassay comprises a further third group comprising a donor for energy transfer to said acceptor, which donor is an up conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound; and said first group comprises a tag, said third group comprises a binder, and said binder has a high affinity for binding to said tag. The fluorescence of said acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• G01N 33/542 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec inhibition stérique ou modification du signal, p. ex. extinction de fluorescence