Abstract

The present invention provides a method for determining a background count rate in liquid scintillation counting. The method comprises measuring external standard spectra of a sample, determining, from the external standard spectra, a triple to double coincidence ratio and a quench parameter, determining, based on the triple to double coincidence ratio and the quench parameter, a background reference parameter, and determining, based on the background reference parameter, the background count rate from a background reference curve.

IPC Classes  ?

• G01T 1/204 - Measuring radiation intensity with scintillation detectors the detector being a liquid
• G01T 1/20 - Measuring radiation intensity with scintillation detectors

Goods & Services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical preparations for analysis in laboratories for use in life science research and radiation measurement; Chemical substances for analyses in laboratories for use in life science research and radiation measurement; Diagnostic reagents, other than for medical laboratory use; Fluorescent dye for scientific or research use; Reagents for laboratory purposes, other than for medical or veterinary purposes; Diagnostic chemicals, other than for medical or veterinary use; Chemical compositions and materials for use in science; Reagents for scientific purposes in life science research, radiation measurement and nuclear medicine; Chemicals for use in scientific research, other than medical or veterinary; Reference standards being chemicals for use in science; Indicators being chemicals for scientific use

Goods & Services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical preparations for analysis in laboratories for use in life science research and radiation measurement; Chemical substances for analyses in laboratories for use in life science research and radiation measurement; Diagnostic reagents, other than for medical laboratory use; Fluorescent dye for scientific or research use; Reagents for laboratory purposes, other than for medical or veterinary purposes; Diagnostic chemicals, other than for medical or veterinary use; Chemical compositions and materials for use in science; Reagents for scientific purposes in life science research, radiation measurement and nuclear medicine; Chemicals for use in scientific research, other than medical or veterinary; Reference standards being chemicals for use in science; Indicators being chemicals for scientific use; all being for life science research, radiation measurement and nuclear medicine

Goods & Services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical substances for analysis in laboratories; Chemical substances for analyses in laboratories, other than for medical or veterinary purposes; Diagnostic reagents, other than for medical laboratory use; Fluorescent chemicals; Reagents for laboratory purposes [other than for medical or veterinary purposes]; Diagnostic chemicals [other than for medical or veterinary use]; Chemicals for use in analysis [other than medical and veterinary]; Chemical compositions and materials for use in science; Reagents for scientific purposes; Chemicals for use in scientific research [other than medical or veterinary]; Reference standards [chemicals] for use in science; Indicators [chemicals] for scientific use.

Goods & Services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical substances for analysis in laboratories; Chemical substances for analyses in laboratories, other than for medical or veterinary purposes; Diagnostic reagents, other than for medical laboratory use; Fluorescent chemicals; Reagents for laboratory purposes [other than for medical or veterinary purposes]; Diagnostic chemicals [other than for medical or veterinary use]; Chemicals for use in analysis [other than medical and veterinary]; Chemical compositions and materials for use in science; Reagents for scientific purposes; Chemicals for use in scientific research [other than medical or veterinary]; Reference standards [chemicals] for use in science; Indicators [chemicals] for scientific use.

Abstract

The present invention provides a method for determining a background count rate in liquid scintillation counting. The method comprises measuring an external standard spectrum (201, 202) of a sample, determining, from the external standard spectrum, an external standard count rate within an energy window (203), determining, based on the external standard count rate within the energy window, a background reference parameter, and determining, based on the background reference parameter, the background count rate from a background reference curve (301).

IPC Classes  ?

• G01T 1/178 - Circuit arrangements not adapted to a particular type of detector for measuring specific activity in the presence of other radioactive substances, e.g. natural, in the air or in liquids such as rain-water
• G01T 1/204 - Measuring radiation intensity with scintillation detectors the detector being a liquid

Goods & Services

Reagents for research purposes; chemical reagents for laboratory use, other than for medical or veterinary use; chemical substances for analysis in laboratories; chemical substances for analyses in laboratories, other than for medical or veterinary purposes; diagnostic reagents, other than for medical laboratory use; fluorescent chemicals; reagents for laboratory purposes [other than for medical or veterinary purposes]; diagnostic chemicals [other than for medical or veterinary use]; chemicals for use in analysis [other than medical and veterinary]; chemical compositions and materials for use in science; reagents for scientific purposes; chemicals for use in scientific research [other than medical or veterinary]; reference standards [chemicals] for use in science; indicators [chemicals] for scientific use. Scientific research and laboratory apparatus, educational apparatus and simulators; laboratory apparatus and instruments; microplate readers; scientific apparatus and instruments; liquid scintillation counters; photometric analyzers [other than for medical use]; measuring, detecting and monitoring instruments, indicators and controllers; luminescence measuring devices; fluorescence analyzers; measuring, counting, alignment and calibrating instruments; optical detection apparatus; luminescence scanners; gamma counters; measuring apparatus and instruments; radiation measuring apparatus; fluorometers; software; application software; operating software; all being for life science research, radiation measurement and nuclear medicine.

Goods & Services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical preparations for analysis in laboratories for use in life science research and radiation measurement; Chemical substances for analyses in laboratories for use in life science research and radiation measurement; Diagnostic reagents, other than for medical laboratory use; Fluorescent dye for scientific or research use; Reagents for laboratory purposes, other than for medical or veterinary purposes; Diagnostic chemicals, other than for medical or veterinary use; Chemical compositions and materials for use in science; Reagents for scientific purposes in life science research, radiation measurement and nuclear medicine; Chemicals for use in scientific research, other than medical or veterinary; Reference standards being chemicals for use in science; Indicators being chemicals for scientific use, all being for life science research, radiation measurement and nuclear medicine Laboratory apparatus and instruments, namely, vials, cuvettes, beakers, filters, swabs and microplates; Microplate readers; Liquid scintillation counters; Photometric analyzers other than for medical use; Luminescence measuring devices; Fluorescence analyzers; Luminescence scanners; Gamma counters; Radiation measuring apparatus; Fluorometers; Downloadable computer software for performing measurement data calculations derived from laboratory, research or scientific instruments; Embedded Recorded software for the operation of microplate readers, liquid scintillation counters, luminescence measuring devices, fluorescence analyzers, luminescence scanners, gamma counters, radiation measuring apparatus and fluorometers; Recorded or downloadable software for the operation of microplate readers, liquid scintillation counters, luminescence measuring devices, fluorescence analyzers, luminescence scanners, gamma counters, radiation measuring apparatus and fluorometers; all being for life science research, radiation measurement and nuclear medicine

Goods & Services

Reagents for research purposes; Chemical reagents for laboratory use, other than for medical or veterinary use; Chemical substances for analysis in laboratories; Chemical substances for analyses in laboratories, other than for medical or veterinary purposes; Diagnostic reagents, other than for medical laboratory use; Fluorescent chemicals; Reagents for laboratory purposes [other than for medical or veterinary purposes]; Diagnostic chemicals [other than for medical or veterinary use]; Chemicals for use in analysis [other than medical and veterinary]; Chemical compositions and materials for use in science; Reagents for scientific purposes; Chemicals for use in scientific research [other than medical or veterinary]; Reference standards [chemicals] for use in science; Indicators [chemicals] for scientific use. Scientific research and laboratory apparatus, educational apparatus and simulators; Laboratory apparatus and instruments; Microplate readers; Scientific apparatus and instruments; Liquid scintillation counters; Photometric analyzers [other than for medical use]; Measuring, detecting and monitoring instruments, indicators and controllers; Luminescence measuring devices; Fluorescence analyzers; Measuring, counting, alignment and calibrating instruments; Detection apparatus; Luminescence scanners; Gamma counters; Measuring apparatus and instruments; Radiation measuring apparatus; Fluorometers; Software; Application software; Operating software; all being for life science research, radiation measurement and nuclear medicine.

Abstract

A homogenous bioassay including i) a first group containing a short lifetime fluorescent acceptor, and ii) a second group containing a quencher, with the first and second groups linked by at least a first linkage. The bioassay measures the acceptor's fluorescence increase resulting from cleavage of the first linkage and also includes iii) a third group containing a donor for energy transfer to the acceptor, where the donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. A conformational or terminal epitope is created on the first group through linkage cleavage, and the third group includes a binder with affinity for this epitope. The acceptor's fluorescence is caused by exciting the donor. Also disclosed are bioassay kits for this method.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

A homogenous bioassay including a first group including an acceptor which is a short lifetime fluorescent compound capable of energy transfer, and a second group including a quencher which is capable of energy transfer from an acceptor. The increase or decrease of the acceptor's fluorescence resulting from lengthening or shortening of the distance between the acceptor and quencher is measured. The bioassay also includes a third group including a donor for energy transfer to the acceptor, which donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. The first group includes a tag, the third group includes a binder having a high affinity for binding to the tag. Fluorescence of the acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical

Abstract

This invention relates to a luminescence energy transfer based homogeneous bioassay wherein measurement of phosphorylated or dephosphorylated peptide or protein is carried out. The bioassay comprises a) a first group labelled with an energy donor; wherein the first group is a particulate comprised of a polymer or an inorganic crystallic structure, and the first group is i) a long-lifetime luminescent lanthanide label if the particulate is a polymer, or ii) an up-conversion luminescent lanthanide label or down-conversion luminescent lanthanide label if the particulate is an inorganic crystallic structure. The first group is able to transfer fluorescence energy to an acceptor of a second group, and the first group i) is coated with a trivalent metal ion binder through passive adsorption, achelate structure or a polymeric structure, or ii) has trivalent metal ions incorporated into the crystallic structure. The second group is labell ed with the energy acceptor, wherein the acceptor is a short-lifetime fluorescent label, and the second group comprises a peptide or protein with at least one phosphorylatable amino acid within 10 nm from said acceptor. After a sample and reagents of the bioassay, the reagents including the first and second groups, and trivalent metalions if not incorporated in the second group, have been brought into contact with each other, the first group is excited and resulting emission of the second group, the emission relating to energy transfer from donor to acceptor due to phosphorylated peptides and/or proteins of the second group being attached to the trivalent metal ions bound to or incorporated into the first group, is measured. The present invention also relates to kits for bioassays according to the invention.

IPC Classes  ?

• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/553 - Metal or metal coated

Abstract

This invention relates to a homogenous bioassay for use in measurement of biological activity, its modulation or analyte concentration of a sample, said bioassay comprising i) a first group comprising an acceptor, which acceptor is a short lifetime fluorescent compound capable of energy transfer, and ii) a second group comprising a quencher, which quencher is capable of energy transfer from an acceptor, and the first and second group are linked by at least a first linkage, and an increase, of fluorescence of said acceptor due to the decrease, of energy transfer from said acceptor to said quencher resulting from cleavage of said first linkage, is measured. Characteristic for the bioassay is that it comprises iii) a further third group comprising a donor for energy transfer to said acceptor, which donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound; and a conformational or terminal epitope is created on said first group through said cleavage of the linkage, and said third group comprises a binder with affinity for binding to said conformational or terminal epitope; and the fluorescence of said acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor. This invention also relates to kits for homogenous bioassays according to the method of the invention.

IPC Classes  ?

• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

A method to correct measurement error in a resonance energy-transfer assay, including exciting anti-Stokes photoluminescent donors with at least one wavelength of light which is greater than an emission wavelength of acceptor molecules; measuring emission at the acceptor's emission wavelength and which differs from the donor's emission wavelength in at least two different time windows; a first time window within the time window defined by the excitation light pulse and a second non-overlapping time window which follows the first time window; and correcting the emission signal, which includes signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission signal measured in the second time window.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence

Abstract

This invention relates to a homogenous bioassay for use in measurement of biological activity, its modulation or analyte concentration of a sample, said bioassay comprising a first group comprising an acceptor, which acceptor is a short lifetime fluorescent compound capable of energy transfer, and a second group comprising a quencher, which quencher is capable of energy transfer from an acceptor. The increase or decrease, respectively, of fluorescence of said acceptor due to the decrease or increase, respectively, of energy transfer from said acceptor to said quencher resulting from lengthening or shortening, respectively, of the distance between said acceptor and quencher is measured. Characteristic for the invention is that the bioassay comprises a further third group comprising a donor for energy transfer to said acceptor, which donor is an up conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound; and said first group comprises a tag, said third group comprises a binder, and said binder has a high affinity for binding to said tag. The fluorescence of said acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching