Abrégé

Provided herein are systems, methods, compositions, and kits for analyte detection. The systems, methods, compositions, and kits can allow for multiplex analysis of multiple analytes. The compositions provided herein comprises various probes, enzymes, label moieties, quencher moieties, or primers for practicing the methods. Additionally, provided herein are analysis methods for analyte detections and the devices and/or systems for practicing the same.

Classes IPC  ?

• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]
• C12Q 1/6851 - Amplification quantitative

Abrégé

A sample processing apparatus, comprising: a housing (101) having at least one chamber, and a valve body (301), the valve body (301) being coupled to the housing (101), the valve body (301) having a fluid displacement chamber (3012), a fluid port (3013), and a fluid channel (3014) fluidly connecting the fluid displacement chamber (3012) and the fluid port (3013), the fluid displacement chamber (3012) being coupled to a pressure adjustment member (1027), and the pressure adjustment member (1027) being configured to change a pressure within the fluid displacement chamber (3012); the valve body (301) being adjustable with respect to the housing (101), such that the fluid port (3013) of the valve body (301) is selectively in fluidic communication with the at least one chamber of the housing (101).

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• G01N 1/38 - Dilution, dispersion ou mélange des échantillons

Abrégé

A sample collection apparatus, comprising: a mouthpiece (101, 301), wherein the mouthpiece (101, 301) is configured to engage with at least a portion of the mouth of a subject; a length-adjustable component (102, 302), wherein a first end of the length-adjustable component (102, 302) is coupled to the mouthpiece (101, 301), and the length-adjustable component (102, 302) has a first state and a second state, wherein in the first state, the length-adjustable component (102, 302) has a first length along the longitudinal axis thereof, and in the second state, the length-adjustable component (102, 302) has a second length along the longitudinal axis, with the second length greater than the first length; and a sampling head (103, 303), wherein the sampling head (103, 303) is coupled to a second end, substantially opposite to the first end along the longitudinal axis, of the length-adjustable component (102, 302), and the sampling head (103, 303) is configured, when the length-adjustable component (102, 302) is in the second state, to contact a collection site in the subject and collect a biological sample from the collection site.

Classes IPC  ?

• A61B 10/00 - Instruments pour le prélèvement d'échantillons corporels à des fins de diagnostic Autres procédés ou instruments pour le diagnostic, p. ex. pour le diagnostic de vaccination ou la détermination du sexe ou de la période d'ovulationInstruments pour gratter la gorge

Produits et services

Germicides; Antiseptic cotton; Chemical preparations for pharmaceutical or medical purposes, namely, for diagnosis of DNA and early screening for cancer cells; Diagnostic preparations for veterinary purposes; Dietary supplements; Disinfectants for hygiene purposes; Mixed biological preparations for the prevention and treatment of infectious diseases; Nutritional supplements

Produits et services

Analysers for bacterial identification for medical purposes; Analyzers for bacterial identification for medical purposes; Apparatus for DNA and RNA testing for medical purposes; Body composition monitors; Genetic testing apparatus for medical purposes; Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing in the fields of cancer or other tissue-based diagnostic testing, cytology and cell-based testing; Medical diagnostic apparatus for testing cancer cells, DNA, incorporating recorded software for diagnosis of DNA and early screening for cancer cells

Produits et services

Alidades; Data processing apparatus; DNA chips; Downloadable virtual reality software for medical teaching purposes; Electronic and optical communications instruments and components, namely, optical transceivers; Juke boxes for computers; Laboratory apparatus and instruments for the exchange of substances and heat; Laboratory centrifuges; Laboratory robots

Abrégé

A sample processing apparatus, comprising: a housing (101) having at least one chamber, and a valve body (301), the valve body (301) being coupled to the housing (101), the valve body (301) having a fluid replacement cavity (3012), a fluid port (3013), and a fluid channel (3014) fluidly connecting the fluid replacement cavity (3012) and the fluid port (3013), the fluid replacement cavity (3012) being coupled to a pressure adjustment apparatus (1027), and the pressure adjustment apparatus (1027) being configured to change the pressure within the fluid replacement cavity (3012); the valve body (301) is adjustable relative to the housing (101) such that the fluid port (3013) of the valve body (301) is selectively in fluid communication with the at least one chamber of the housing (101).

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

The present invention provides methods and systems for amplifying and analyzing nucleic acid samples. The method may comprise activating a system comprising a fluid flow network, generating partitions, subjecting the partitions to conditions sufficient to perform a nucleic acid amplification reaction, and detecting signals indicative of a presence or absence of amplification products. Systems to perform the method may comprise a fluid flow network, a plurality of thermal zones, and a detector.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

The present disclosure provides methods and systems for sample analysis. Specifically, the present disclosure provides methods, apparatus and systems for loading a biological sample from its container to the electrophoretic apparatus in a closed system such that the risks of contamination of the sample and biohazard caused by leakage of the sample are greatly reduced.

Classes IPC  ?

• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12M 1/26 - Inoculateur ou échantillonneur
• G01N 27/447 - Systèmes utilisant l'électrophorèse
• G01N 27/44 - Mesure du dépôt ou de la libération de matériaux d'un électrolyteCoulométrie, c.-à-d. mesure de l'équivalent de Coulomb du matériau dans un électrolyte utilisant l'électrolyse pour engendrer un réactif, p. ex. pour un dosage
• G01N 27/00 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques

Abrégé

The present disclosure provides devices, methods and kits for collecting a sample of a subject for nucleic acid amplification. The device may comprise: a collection vessel that can include reagents necessary for nucleic acid amplification, wherein the collection vessel may be adapted to accept at least one swab containing a sample; a cap integrated with the collection vessel and having a channel extending therethrough to permit the at least one swab to be deposited in the collection vessel, wherein the channel is closable upon rotation of the cap; and at least one cutting member that severs a longitudinal portion of a stem of the swab extending through said channel upon rotation of the cap.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• G01N 1/02 - Dispositifs pour prélever des échantillons
• A61B 10/02 - Instruments pour prélever des échantillons cellulaires ou pour la biopsie
• G01N 1/38 - Dilution, dispersion ou mélange des échantillons
• G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
• A61B 10/00 - Instruments pour le prélèvement d'échantillons corporels à des fins de diagnostic Autres procédés ou instruments pour le diagnostic, p. ex. pour le diagnostic de vaccination ou la détermination du sexe ou de la période d'ovulationInstruments pour gratter la gorge

Abrégé

Disclosed methods and systems for analyzing nucleic acids and for conducting chemical and/or biological reactions. Disclosed methods and system for droplet generation, guidance, and isolation.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
• B01J 13/00 - Chimie des colloïdes, p. ex. production de substances colloïdales ou de leurs solutions, non prévue ailleursFabrication de microcapsules ou de microbilles
• C08J 3/00 - Procédés pour le traitement de substances macromoléculaires ou la formation de mélanges

Abrégé

Nucleic acid amplification systems and methods for performing nucleic acid amplifications with such systems are provided.

Classes IPC  ?

• C12M 1/38 - Commande sensible à la température
• C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present disclosure provides methods and systems for amplifying nucleic acid samples. In some aspects, the methods and systems provided can be useful in conducting multiple nucleic acid amplification reactions in parallel. In some embodiments, methods and systems provided herein can be useful in conducting reverse transcription and DNA amplification in parallel. Moreover, in some aspects, the methods and systems described herein can be useful in analysis of nucleic acid samples. In some embodiments, methods and systems provided herein can be useful for conducting multiple series of primer extension reactions, which can aid in analysis of a nucleic acid sample.

Classes IPC  ?

• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

Provided herein are devices, systems, and methods for conducting electrophoresis. The devices, systems, and methods are suited for portability, low power consumption, integrated operation, and remote monitoring.

Classes IPC  ?

• G01N 27/447 - Systèmes utilisant l'électrophorèse

Abrégé

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages

Abrégé

Provided are methods and systems for amplifying and analyzing nucleic acid samples.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

Devices, methods and kits for collecting a sample of a subject for nucleic acid amplification. The device (202) may comprise: a collection vessel (2024) that can include reagents (2023) necessary for nucleic acid amplification, wherein the collection vessel (2024) may be adapted to accept at least one swab (201) containing a sample; a cap (2021, 2022) integrated with the collection vessel (2024) and having a channel extending therethrough to permit the at least one swab (201) to be deposited in the collection vessel (2024), wherein the channel is closable upon rotation of the cap (2021, 2022); and at least one cutting member (4033) that severs a longitudinal portion of a stem (2011) of the swab (201) extending through said channel upon rotation of the cap (2021, 2022).

Classes IPC  ?

• C12M 1/26 - Inoculateur ou échantillonneur
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present disclosure provides a system for collecting and/or processing a bodily fluid sample of a subject, a method for collecting and/or processing a bodily fluid sample of a subject, and a kit for collecting and/or processing a bodily fluid sample of a subject.

Classes IPC  ?

• C12M 1/26 - Inoculateur ou échantillonneur
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present disclosure provides methods and systems for assaying the presence of a target nucleic acid molecule in a sample having or suspected of having the target nucleic acid molecule. A method of assaying the presence of the target nucleic acid molecule comprises facilitating the flow of the sample through at least one nanopore in a membrane disposed adjacent or in proximity to an electrode that detects a current or current change upon movement of the target nucleic acid molecule through the nanopore. The target nucleic acid molecule， if present， can have a tag coupled at a terminal end thereof that increases a dwell time of the target nucleic acid molecule in the nanopore. The presence of the target nucleic acid molecule in the sample is assayed based on an increase in dwell time of the target nucleic acid molecule from measurements of the current or current change.

Classes IPC  ?

• G01N 27/406 - Cellules et sondes avec des électrolytes solides
• G01N 27/414 - Transistors à effet de champ sensibles aux ions ou chimiques, c.-à-d. ISFETS ou CHEMFETS
• G01N 27/417 - Systèmes utilisant des cellules et des sondes à électrolyte solide
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

An apparatus and a method for conducting a chemical reaction in a sample contained in a sample holder, said reaction requiring cycling between at least two target temperature levels. The method comprises: (a) placing said sample holder in thermal contact with a first overshooting thermal zone to achieve a first target temperature level; (b) placing said sample holder in thermal contact with a second overshooting thermal zone to achieve a second target temperature level; and optionally repeating steps (a) and (b); wherein said first overshooting thermal zone is at a higher temperature than said first target temperature level, and said second overshooting thermal zone is at a lower temperature than said second target temperature level. One or more rotating arms is programmed to place said sample holder in thermal contact with one or more of said thermal zones. Said reaction is a polymerase chain reaction (PCR).

Classes IPC  ?

• C12M 1/38 - Commande sensible à la température

Abrégé

Provided herein are devices, systems, and methods for conducting nucleic acid amplification. The devices, systems, and methods are suited for portability, rapid operation, low power consumption, integrated operation, and remote monitoring.

Classes IPC  ?

• C12M 1/38 - Commande sensible à la température
• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• F27D 11/00 - Aménagement des éléments pour le chauffage électrique dans ou sur les fours

Abrégé

The present disclosure provides methods and systems for assaying the presence of a target nucleic acid molecule in a sample having or suspected of having the target nucleic acid molecule. A method of assaying the presence of the target nucleic acid molecule comprises facilitating the flow of the sample through at least one nanopore in a membrane disposed adjacent or in proximity to an electrode that is adapted to detect a current or change thereof upon movement of a complex having the target nucleic acid molecule coupled to the complexing moiety through the at least one nanopore. Next, the current or change thereof is measured with the electrode. The complex in the sample is detected from the current or change thereof, thereby assaying the presence of the target nucleic acid molecule in the sample.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• G01N 27/26 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables électrochimiquesRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en utilisant l'électrolyse ou l'électrophorèse
• G01N 27/403 - Ensembles de cellules et d'électrodes

Abrégé

The present disclosure provides methods and systems for amplifying nucleic acid samples. In some aspects, the methods and systems described provided can be useful in conducting multiple nucleic acid amplification reactions in parallel. In some embodiments, methods and systems provided herein can be useful in conducting reverse transcription and DNA amplification in parallel. Moreover, in some aspects, the methods and systems described herein can be useful in analysis of nucleic acid samples. In some embodiments, methods and systems provided herein can be useful for conducting multiple series of primer extension reactions, which can aid in analysis of a nucleic acid sample.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
• G01N 21/64 - FluorescencePhosphorescence
• B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique

Abrégé

An apparatus for performing electrophoresis on a sample comprises: a frame comprising a bottom plate and a plurality of side walls; an electrophoresis gel retained in said frame and an electrical component that produces an electric field through the electrophoresis gel to cause at least a portion of the sample to migrate through the gel, wherein the electrical component is powered by no more than 12 V. A method for performing electrophoresis is also disclosed. The apparatus is suited for portability, low power consumption, integrated operation, and remote monitoring.

Classes IPC  ?

• G01N 27/447 - Systèmes utilisant l'électrophorèse

Produits et services

[ Apparatus for testing gas, liquids and solids; Bio-chips for research or scientific purposes; Diagnostic apparatus for testing food; Diagnostic apparatus for the detection of pathogens for laboratory or research use; DNA chips; Electrophoresis apparatus not for medical purposes; Optical inspection apparatus; ] Scientific instrumentation for measuring real-time quantitative polymerase chain reactions not for medical use [ ; Scientific instruments, namely, electronic analyzers for testing and analyzing chemical and biological substances for the presence, absence, or quantity of biologics; Scientific apparatus and instruments for measuring relative DNA, RNA and protein and parts and fittings therefor ]

Abrégé

Systems and methods for low voltage power thermal cycling are provided. A nucleic acid amplification device comprising a thermal cycler (110) and a detector (120) is capable of operating using a low voltage value, such asl2V.The device may include a portable energy storage device that can be used to provide power to the device. The nucleic acid amplification device may be powered by a vehicle (1120) and deployed to remote locations.

Classes IPC  ?

• C12M 1/38 - Commande sensible à la température

Abrégé

Systems and methods for low voltage power thermal cycling are provided. A nucleic acid amplification device (500) comprising a thermal cycler (510) and a detector (520) is capable of operating using a low operating voltage, such as 12 V. The nucleic acid amplification device (500) may include a portable energy storage device (530) that can provide operating power. The nucleic acid amplification device (500) may be powered by a vehicle (1120) and deployed to remote locations.

Classes IPC  ?

• C12M 1/38 - Commande sensible à la température

Abrégé

Lid configurations for thermal cyclers and uses thereof are provided. A nucleic acid amplification device may include a lid assembly that may be able to move between an open and closed configuration. The lid assembly may include a heater therein. The heater in the lid may have an adjustable configuration that may be able to accommodate different configurations of sample containers. Optionally, the heater may be retractable within a housing of the thermal cycler, when the lid is opened.

Classes IPC  ?

• C12M 1/38 - Commande sensible à la température
• F27D 11/00 - Aménagement des éléments pour le chauffage électrique dans ou sur les fours
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

Classes IPC  ?

• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• G01N 21/64 - FluorescencePhosphorescence

Abrégé

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
• G06F 19/22 - pour la comparaison de séquences impliquant des nucléotides ou des acides aminés, p.ex. recherche d'homologie, identification de motifs ou de polymorphismes de nucléotides simples [SNP] ou alignement de séquences