Provided are a group of biomarkers for diagnosing hypertension in children, a test kit, and a use thereof. By means of collecting tongue coating/intestinal samples of obese children with hypertension, obese children, and healthy people, performing metagenomic sequencing, and using bioinformatics to calculate statistics on sequencing data, disease-related tongue/intestinal flora is found, and the tongue/intestinal flora is integrated with disease information to obtain a combination of flora biomarkers. A binary classification prediction model constructed by means of said combination can maximally detect obese children with hypertension.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
G16B 25/00 - ICT specially adapted for hybridisationICT specially adapted for gene or protein expression
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indicesICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
2.
LIQUID TRANSFER APPARATUS, LIQUID DISPENSING SYSTEM AND LIQUID DISPENSING METHOD
The present disclosure provides a liquid transfer apparatus, comprising a dispensing control component and a reagent cartridge. The dispensing control component comprises: an injection port; multiple branch channels, each branch channel having one end in communication with the injection port, and another end being a branch channel terminal; multiple reaction chambers, each comprising a reaction chamber body, and a reaction chamber inlet in communication with the reaction chamber body, each reaction chamber corresponding to a branch channel, and the reaction chamber inlet being spaced apart from the corresponding branch channel terminal. The reagent cartridge comprises a reagent storage chamber internally storing a reagent, and further comprises a reagent cartridge inlet and a reagent cartridge outlet that place the reagent storage chamber in communication with the exterior of the reagent cartridge. The reagent cartridge and the dispensing control component are two independent components, and the reagent cartridge is configured to be detachably fitted with the dispensing control component, causing a branch channel terminal to be in communication with a reagent cartridge inlet, and the reagent cartridge outlet to be in communication with a reaction chamber inlet corresponding to the branch channel terminal, forming a flow path from the injection port, through the branch channel and the reagent cartridge, to the reaction chamber.
The present disclosure provides a pipetting device, comprising a plurality of tube bodies and a dispensing control portion. Each tube body is internally provided with an accommodating space. The dispensing control portion comprises a plurality of tube cover portions, configured to match the plurality of tube bodies on a one-to-one basis to close the accommodating spaces, the tube cover portions being provided with inlet tubes that are communicated with the accommodating spaces; and a liquid dispensing portion, configured to be detachably connected to the tube cover portions, the liquid dispensing portion comprising an injection port, a plurality of branch flow channels, and a plurality of valves. Liquid can be injected into the dispensing control portion through the injection port. Each of the plurality of branch flow channels has one end communicated with the injection port, and the other end that can be communicated with the inlet tube of one of the plurality of tube cover portions. Each branch flow channel of the plurality of branch flow channels is provided with at least one valve, and the valve is configured to be switchable between an open state and a closed state, so as to respectively control the opening and closing of the corresponding branch flow channel.
Provided in the present disclosure is a storage device capable of pre-loading a plurality of trace reagents. The device can realize co-mixing of trace liquids during use. This provides great convenience for quick operations such as rapid on-site testing, one-step reagent addition and detection range expansion during integrated sampling and testing. The storage device comprises a first storage component having a first chamber and a second storage component having a second chamber, and is configured such that the storage device transitions from a first storage state to a mixed state by means of moving a first end portion of the second storage component in a first direction toward the interior of the first chamber. In the first storage state, the first end portion of the second storage component is configured to be accommodated in a fitting end portion, and the first chamber is isolated from the second chamber; and in the mixed state, a first outlet on the first end portion of the second storage component extends into the first chamber, and the first chamber is in communication with the second chamber. Further provided are a storage and mixing system comprising the storage device, and a method for storing and mixing substances by using the storage device.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B65D 81/32 - Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents for packaging two or more different materials which must be maintained separate prior to use in admixture
5.
COMPOSITIONS AND METHODS FOR NUCLEIC ACID DETECTION
Provided herein are systems, methods, compositions, and kits for analyte detection. The systems, methods, compositions, and kits can allow for multiplex analysis of multiple analytes. The compositions provided herein comprises various probes, enzymes, label moieties, quencher moieties, or primers for practicing the methods. Additionally, provided herein are analysis methods for analyte detections and the devices and/or systems for practicing the same.
A sample processing apparatus, comprising: a housing (101) having at least one chamber, and a valve body (301), the valve body (301) being coupled to the housing (101), the valve body (301) having a fluid displacement chamber (3012), a fluid port (3013), and a fluid channel (3014) fluidly connecting the fluid displacement chamber (3012) and the fluid port (3013), the fluid displacement chamber (3012) being coupled to a pressure adjustment member (1027), and the pressure adjustment member (1027) being configured to change a pressure within the fluid displacement chamber (3012); the valve body (301) being adjustable with respect to the housing (101), such that the fluid port (3013) of the valve body (301) is selectively in fluidic communication with the at least one chamber of the housing (101).
A sample collection apparatus, comprising: a mouthpiece (101, 301), wherein the mouthpiece (101, 301) is configured to engage with at least a portion of the mouth of a subject; a length-adjustable component (102, 302), wherein a first end of the length-adjustable component (102, 302) is coupled to the mouthpiece (101, 301), and the length-adjustable component (102, 302) has a first state and a second state, wherein in the first state, the length-adjustable component (102, 302) has a first length along the longitudinal axis thereof, and in the second state, the length-adjustable component (102, 302) has a second length along the longitudinal axis, with the second length greater than the first length; and a sampling head (103, 303), wherein the sampling head (103, 303) is coupled to a second end, substantially opposite to the first end along the longitudinal axis, of the length-adjustable component (102, 302), and the sampling head (103, 303) is configured, when the length-adjustable component (102, 302) is in the second state, to contact a collection site in the subject and collect a biological sample from the collection site.
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
8.
SAMPLE PROCESSING APPARATUS AND SAMPLE PROCESSING METHOD
A sample processing apparatus, comprising: a housing (101) having at least one chamber, and a valve body (301), the valve body (301) being coupled to the housing (101), the valve body (301) having a fluid replacement cavity (3012), a fluid port (3013), and a fluid channel (3014) fluidly connecting the fluid replacement cavity (3012) and the fluid port (3013), the fluid replacement cavity (3012) being coupled to a pressure adjustment apparatus (1027), and the pressure adjustment apparatus (1027) being configured to change the pressure within the fluid replacement cavity (3012); the valve body (301) is adjustable relative to the housing (101) such that the fluid port (3013) of the valve body (301) is selectively in fluid communication with the at least one chamber of the housing (101).
The present invention provides methods and systems for amplifying and analyzing nucleic acid samples. The method may comprise activating a system comprising a fluid flow network, generating partitions, subjecting the partitions to conditions sufficient to perform a nucleic acid amplification reaction, and detecting signals indicative of a presence or absence of amplification products. Systems to perform the method may comprise a fluid flow network, a plurality of thermal zones, and a detector.
The present disclosure provides methods and systems for sample analysis. Specifically, the present disclosure provides methods, apparatus and systems for loading a biological sample from its container to the electrophoretic apparatus in a closed system such that the risks of contamination of the sample and biohazard caused by leakage of the sample are greatly reduced.
G01N 27/44 - Measuring deposition or liberation of materials from an electrolyteCoulometry, i.e. measuring coulomb-equivalent of material in an electrolyte using electrolysis to generate a reagent, e.g. for titration
G01N 27/00 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
The present disclosure provides devices, methods and kits for collecting a sample of a subject for nucleic acid amplification. The device may comprise: a collection vessel that can include reagents necessary for nucleic acid amplification, wherein the collection vessel may be adapted to accept at least one swab containing a sample; a cap integrated with the collection vessel and having a channel extending therethrough to permit the at least one swab to be deposited in the collection vessel, wherein the channel is closable upon rotation of the cap; and at least one cutting member that severs a longitudinal portion of a stem of the swab extending through said channel upon rotation of the cap.
A61B 10/02 - Instruments for taking cell samples or for biopsy
G01N 1/38 - Diluting, dispersing or mixing samples
G01N 33/487 - Physical analysis of biological material of liquid biological material
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
12.
METHODS AND SYSTEMS FOR CONDUCTING CHEMICAL OR BIOLOGICAL REACTION
Disclosed methods and systems for analyzing nucleic acids and for conducting chemical and/or biological reactions. Disclosed methods and system for droplet generation, guidance, and isolation.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
B01J 13/00 - Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided forMaking microcapsules or microballoons
C08J 3/00 - Processes of treating or compounding macromolecular substances
The present disclosure provides methods and systems for amplifying nucleic acid samples. In some aspects, the methods and systems provided can be useful in conducting multiple nucleic acid amplification reactions in parallel. In some embodiments, methods and systems provided herein can be useful in conducting reverse transcription and DNA amplification in parallel. Moreover, in some aspects, the methods and systems described herein can be useful in analysis of nucleic acid samples. In some embodiments, methods and systems provided herein can be useful for conducting multiple series of primer extension reactions, which can aid in analysis of a nucleic acid sample.
Provided herein are devices, systems, and methods for conducting electrophoresis. The devices, systems, and methods are suited for portability, low power consumption, integrated operation, and remote monitoring.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
19.
METHODS AND SYSTEMS FOR NUCLEIC ACID AMPLIFICATION
Devices, methods and kits for collecting a sample of a subject for nucleic acid amplification. The device (202) may comprise: a collection vessel (2024) that can include reagents (2023) necessary for nucleic acid amplification, wherein the collection vessel (2024) may be adapted to accept at least one swab (201) containing a sample; a cap (2021, 2022) integrated with the collection vessel (2024) and having a channel extending therethrough to permit the at least one swab (201) to be deposited in the collection vessel (2024), wherein the channel is closable upon rotation of the cap (2021, 2022); and at least one cutting member (4033) that severs a longitudinal portion of a stem (2011) of the swab (201) extending through said channel upon rotation of the cap (2021, 2022).
The present disclosure provides a system for collecting and/or processing a bodily fluid sample of a subject, a method for collecting and/or processing a bodily fluid sample of a subject, and a kit for collecting and/or processing a bodily fluid sample of a subject.
The present disclosure provides methods and systems for assaying the presence of a target nucleic acid molecule in a sample having or suspected of having the target nucleic acid molecule. A method of assaying the presence of the target nucleic acid molecule comprises facilitating the flow of the sample through at least one nanopore in a membrane disposed adjacent or in proximity to an electrode that detects a current or current change upon movement of the target nucleic acid molecule through the nanopore. The target nucleic acid molecule， if present， can have a tag coupled at a terminal end thereof that increases a dwell time of the target nucleic acid molecule in the nanopore. The presence of the target nucleic acid molecule in the sample is assayed based on an increase in dwell time of the target nucleic acid molecule from measurements of the current or current change.
An apparatus and a method for conducting a chemical reaction in a sample contained in a sample holder, said reaction requiring cycling between at least two target temperature levels. The method comprises: (a) placing said sample holder in thermal contact with a first overshooting thermal zone to achieve a first target temperature level; (b) placing said sample holder in thermal contact with a second overshooting thermal zone to achieve a second target temperature level; and optionally repeating steps (a) and (b); wherein said first overshooting thermal zone is at a higher temperature than said first target temperature level, and said second overshooting thermal zone is at a lower temperature than said second target temperature level. One or more rotating arms is programmed to place said sample holder in thermal contact with one or more of said thermal zones. Said reaction is a polymerase chain reaction (PCR).
Provided herein are devices, systems, and methods for conducting nucleic acid amplification. The devices, systems, and methods are suited for portability, rapid operation, low power consumption, integrated operation, and remote monitoring.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
F27D 11/00 - Arrangement of elements for electric heating in or on furnaces
25.
DETECTION OF NUCLEIC ACID MOLECULES USING NANOPORES AND COMPLEXING MOIETIES
The present disclosure provides methods and systems for assaying the presence of a target nucleic acid molecule in a sample having or suspected of having the target nucleic acid molecule. A method of assaying the presence of the target nucleic acid molecule comprises facilitating the flow of the sample through at least one nanopore in a membrane disposed adjacent or in proximity to an electrode that is adapted to detect a current or change thereof upon movement of a complex having the target nucleic acid molecule coupled to the complexing moiety through the at least one nanopore. Next, the current or change thereof is measured with the electrode. The complex in the sample is detected from the current or change thereof, thereby assaying the presence of the target nucleic acid molecule in the sample.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
G01N 27/26 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variablesInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by using electrolysis or electrophoresis
The present disclosure provides methods and systems for amplifying nucleic acid samples. In some aspects, the methods and systems described provided can be useful in conducting multiple nucleic acid amplification reactions in parallel. In some embodiments, methods and systems provided herein can be useful in conducting reverse transcription and DNA amplification in parallel. Moreover, in some aspects, the methods and systems described herein can be useful in analysis of nucleic acid samples. In some embodiments, methods and systems provided herein can be useful for conducting multiple series of primer extension reactions, which can aid in analysis of a nucleic acid sample.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
An apparatus for performing electrophoresis on a sample comprises: a frame comprising a bottom plate and a plurality of side walls; an electrophoresis gel retained in said frame and an electrical component that produces an electric field through the electrophoresis gel to cause at least a portion of the sample to migrate through the gel, wherein the electrical component is powered by no more than 12 V. A method for performing electrophoresis is also disclosed. The apparatus is suited for portability, low power consumption, integrated operation, and remote monitoring.
09 - Scientific and electric apparatus and instruments
Goods & Services
[ Apparatus for testing gas, liquids and solids; Bio-chips for research or scientific purposes; Diagnostic apparatus for testing food; Diagnostic apparatus for the detection of pathogens for laboratory or research use; DNA chips; Electrophoresis apparatus not for medical purposes; Optical inspection apparatus; ] Scientific instrumentation for measuring real-time quantitative polymerase chain reactions not for medical use [ ; Scientific instruments, namely, electronic analyzers for testing and analyzing chemical and biological substances for the presence, absence, or quantity of biologics; Scientific apparatus and instruments for measuring relative DNA, RNA and protein and parts and fittings therefor ]
Systems and methods for low voltage power thermal cycling are provided. A nucleic acid amplification device comprising a thermal cycler (110) and a detector (120) is capable of operating using a low voltage value, such asl2V.The device may include a portable energy storage device that can be used to provide power to the device. The nucleic acid amplification device may be powered by a vehicle (1120) and deployed to remote locations.
Systems and methods for low voltage power thermal cycling are provided. A nucleic acid amplification device (500) comprising a thermal cycler (510) and a detector (520) is capable of operating using a low operating voltage, such as 12 V. The nucleic acid amplification device (500) may include a portable energy storage device (530) that can provide operating power. The nucleic acid amplification device (500) may be powered by a vehicle (1120) and deployed to remote locations.
Lid configurations for thermal cyclers and uses thereof are provided. A nucleic acid amplification device may include a lid assembly that may be able to move between an open and closed configuration. The lid assembly may include a heater therein. The heater in the lid may have an adjustable configuration that may be able to accommodate different configurations of sample containers. Optionally, the heater may be retractable within a housing of the thermal cycler, when the lid is opened.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
G06F 19/22 - for sequence comparison involving nucleotides or amino acids, e.g. homology search, motif or Single-Nucleotide Polymorphism [SNP] discovery or sequence alignment
