The present disclosure provides for processes and systems that rely on software, bioinformatics, and robotics principles for actuating the automation of a workflow from nucleic acid extraction from a sample to sequencing without human intervention in between the aforementioned steps.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
2.
FULLY AUTOMATED SAMPLE PROCESSING PLATFORM FROM EXTRACTION TO SEQUENCING
The present disclosure provides for processes and systems that rely on software, bioinformatics, and robotics principles for actuating the automation of a workflow from nucleic acid extraction from a sample to sequencing without human intervention in between the aforementioned steps.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
Provided herein are automated apparatus and methods for the identification of microorganisms in various samples. The disclosure solves existing challenges encountered in identifying and distinguishing various types of microorganisms, including viruses and bacteria in a timely, efficient, and automated manner by library preparation and sequencing.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G16B 25/20 - Réaction en chaîne par polyméraseConception d’amorces ou de sondesOptimisation de la sonde
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
4.
Systems and processes for distinguishing pathogenic and non-pathogenic sequences from specimens
Provided herein are fully-automated next-generation sequencing platforms and processes for detection of a target specimen (e.g., SARS-CoV-2) and for distinguishing infectious from non-infectious signals from the specimen. An analysis can provide simultaneous diagnosis and genomic surveillance of a multitude of distinct specimens in a sample information. The analysis can comprise distinguishing between infectious versus infectious specimens and provide a recommendation as to how infectious the sample can be. The information on can be used to better inform the status of a subject or a location with regards to infectivity from the specimen.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Food processing facilities should employ environmental sampling programs to monitor for general levels of hygiene (the efficacy of general cleaning and sanitation for the removal of transient microorganisms). The instant disclosure provides kits, systems and methods for amplifying a portion of a genome of a pathogen at a plurality of physical locations within a facility; and associating, via a computer, the presence of said pathogen with a location of the plurality of physical locations within said facility.
Provided herein are fully-automated next-generation sequencing platforms and processes for detection of a target specimen (e.g., SARS-CoV-2) and for distinguishing infectious from non-infectious signals from the specimen. An analysis can provide simultaneous diagnosis and genomic surveillance of a multitude of distinct specimens in a sample information. The analysis can comprise distinguishing between infectious versus infectious specimens and provide a recommendation as to how infectious the sample can be. The information can be used to better inform the status of a subject or a location with regards to infectivity from the specimen.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Provided herein are automated apparatus for the identification of microorganisms in various samples. The disclosure solves existing challenges encountered in identifying and distinguishing various types of microorganisms, including viruses and bacteria in a timely, efficient, and automated manner by sequencing.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
8.
Automated nucleic acid library preparation and sequencing device
Provided herein are automated apparatus for the identification of microorganisms in various samples. The disclosure solves existing challenges encountered in identifying and distinguishing various types of microorganisms, including viruses and bacteria in a timely, efficient, and automated manner by sequencing.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
Food processing facilities should employ environmental sampling programs to monitor for general levels of hygiene (the efficacy of general cleaning and sanitation for the removal of transient microorganisms).
05 - Produits pharmaceutiques, vétérinaires et hygièniques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Laboratory supplies for testing pathogens, bacteria, viruses, fungi, protozoa, namely, sample collection kits comprised of testing sample collection equipment in the nature of pre-moistened medicated wipes and medicated swabs for use in diagnostic testing, for medical purposes and laboratory storage tubes Rental of laboratory apparatus, namely, robotic workstations having
multi-use work surfaces for use in a wide variety of fields,
thermocyclers, DNA sequencers, on-deck magnets for testing for
pathogens, bacteria, viruses, fungi, protozoa, and algae
09 - Appareils et instruments scientifiques et électriques
Produits et services
Laboratory apparatus and instruments, namely, on-site robotic platforms in the nature of automated pipetting and thermal cyclers for laboratory analysis for polymerase chain reaction DNA sequencing, culturing, antigen-based testing and automated testing and analysis; Laboratory apparatus and instruments, namely, on-site robotic platforms in the nature of automated pipetting and thermal cyclers for laboratory analysis for identifying, characterizing, and subtyping genetic pathogen sequences for food safety testing
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
laboratory services, namely, providing genetic testing services for research purposes through the genetic fingerprinting of bacteria from environmental surfaces and the comparison of the fingerprinting data to historical data
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Scientific laboratory testing services for the
identification of the presence and absence of ingredients
and constituents of food and beverages; product testing for
purity; product testing, namely, testing for the presence of
heavy metals, toxins, allergens, additives, contaminants,
hormones, pesticides, antibiotics, and genetically modified
organisms; testing for nutritional data and product
authenticity; providing on-site robotic platforms for PCR,
DNA sequencing, culturing, antigen-based testing, automated
testing and analysis, including thermal cyclers, and related
supplies including reagents, primers, DNA probes,
oligonucleotides, pipette tips, enzymes, polymerases (term
considered too vague by the International Bureau - rule 13
(2) (b) of the Common Regulations).
14.
Detection of microorganisms in food samples and food processing facilities
Escherichia genera in a timely and efficient manner. The disclosure also provides methods for differentiating a transient versus a resident pathogen, correlating presence of non-pathogenic with pathogenic microorganisms, distinguishing live versus dead microorganisms by sequencing.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
Escherichia genera in a timely and efficient manner. The disclosure also provides methods for differentiating a transient versus a resident pathogen, correlating presence of non-pathogenic with pathogenic microorganisms, distinguishing live versus dead microorganisms by sequencing.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
Provided herein are methods and apparatus for the identification of pathogenic and non-pathogenic microorganisms in food and environmental samples. The disclosure solves existing challenges encountered in identifying food borne pathogens, including pathogens of the Salmonella, Campylobacter, Listeria, and Escherichia genera in a timely and efficient manner. The disclosure also provides methods for differentiating a transient versus a resident pathogen, correlating presence of non-pathogenic with pathogenic microorganisms, distinguishing live versus dead microorganisms by sequencing.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Providing quality control services to food and beverage manufactures in the nature of sampling surfaces for contaminants and pathogens in the nature of bacteria, viruses and other foreign substances, the identification and analysis of samples, and providing reports identifying the nature and locations of contaminants on food processing, packaging and handling equipment
18.
Detection of microorganisms in food samples and food processing facilities
Escherichia genera in a timely and efficient manner. The disclosure also provides methods for differentiating a transient versus a resident pathogen, correlating presence of non-pathogenic with pathogenic microorganisms, distinguishing live versus dead microorganisms by sequencing.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
19.
Detection of microorganisms in food samples and food processing facilities
Escherichia genera in a timely and efficient manner. The disclosure also provides methods for differentiating a transient versus a resident pathogen, correlating presence of non-pathogenic with pathogenic microorganisms, distinguishing live versus dead microorganisms by sequencing.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Scientific laboratory testing services for the identification of the presence and absence of ingredients and constituents of food and beverages; Product testing for purity; Product testing, namely, testing for the presence of heavy metals, toxins, allergens, additives, contaminants, hormones, pesticides, antibiotics, and genetically modified organisms; Food safety testing for nutritional data and product authenticity; Rental of laboratory apparatus and instruments, namely, on-site robotic platforms for laboratory analysis, namely, for PCR DNA sequencing, culturing, antigen-based testing and automated testing and analysis including thermal cyclers
21.
APPARATUS AND METHOD FOR ECONOMIC, FAST AND EASY SAMPLING OF FOOD AND ENVIRONMENTAL SAMPLES
A handheld solid sample pipette device includes a pipette tube with a number of sharpened teeth adapted to pierce solid or substantially solid sample material, the pipette tube adapted to be matingly attached to a device that includes a handle, a plunger, and a piston within a shell of the device. A spring may be positioned to return the plunger to a non-depressed position following depression of the plunger. A sample of the material can be ejected from the handheld device. The handheld device may further include a shear ring apparatus, that in-turn includes a tube portion, a plurality of shear ring teeth, and a knobbed plate for rotating the shear ring. Following insertion of the pipette with sharpened teeth into the sample, the shear ring apparatus is pressed into the sample as well, such that the shear ring tube, which is slidingly engaged about the pipette tube, slides over the pipette tube into the sample. The shear ring teeth pierce the sample, and at a certain point of insertion, the shear ring apparatus is rotated in either or both a clockwise or counter-clockwise manner such that the shear ring teeth sever a portion of the material to be sampled such that the portion remains within the pipette tube, for removal, and analysis.
