Apparatus, methods, and systems including a syringe for delivering a fluid. The syringe includes a syringe body, a piston, an insert, and a removable plunger. The piston is located in a lumen of the syringe body such that a bottom of the piston together with interior walls of the syringe define a working volume. The piston comprising a cavity. The insert is located at a desired position in the lumen. The insert narrowing the lumen and being configured to prevent retraction of the piston beyond the desired position. The removable plunger is configured to removably couple to the piston. The removable plunger includes a tip configured to couple with the cavity of the piston and move the piston when a force is applied at the plunger, and to decouple from the piston when the piston engages the insert at the desired position.
Systems or techniques are provided for facilitating retrieval augmented generative question and answer boosting. In various embodiments, a system can access a plain text question regarding a scientific instrument. In various aspects, the system can generate, via a large language model that references a document-graph repository, a structured or unstructured answer for the plain text question. In various instances, the document-graph repository can comprise a plurality of document-graphs that respectively correspond to a plurality of technical documents. In various cases, for a first document-graph that corresponds to a first technical document, leaf nodes of the first document-graph can represent respective text blocks written in the first technical document, and non-leaf nodes of the first document-graph can respectively represent a document title, one or more section headings, and one or more scientific instrument identifiers written in the first technical document and beneath which the respective text blocks are nested.
The present disclosure relates to N-protected NH-rhodanine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays.
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C09B 11/24 - Phtaléines contenant des groupes amine
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
6.
Systems and Methods for Performing Data-Dependent Tandem Mass Spectrometry
An illustrative method of performing data-dependent tandem mass spectrometry includes a mass spectrometer acquiring an MS1 mass spectrum of ions produced from a sample, determining, based on the MS1 mass spectrum, a set of observed precursor ions, determining expected fragment ions for the set of observed precursor ions, and acquiring one or more MS2 mass spectra for select ions observed in the MS1 mass spectrum, wherein the expected fragment ions are excluded from being selected for MS2 analysis.
Sample preparation compositions and methods for purifying plasmid DNA from biological samples is provided, are provided. The compositions and methods provided herein allow pDNA analysis to be carried out without centrifugation. The preparation process is amenable to high throughput processing using manual or robotic platforms.
Described herein are compositions, methods, and kits for detecting antibiotic resistance genes in a sample, such as a wound swab. One embodiment described herein is primer pairs and probes for individual or multiplex polymerase chain reaction (PCR) based assays for the detection of one or more antibiotic resistance gene targets comprising resistance to molecularly characterized extended-spectrum β-lactamases (MESBLs); extended-spectrum β-lactamases (ESBLs); carbapenemase; AmpC β-lactamase; β-lactamase; lincosamide, macrolide, streptogramin; trimethoprim; macrolides; methicillin; colistin; sulfonamide; tetracycline; vancomycin; nitroimidazole; quinolone; or aminoglycoside.
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
Thermo Scientific Portable Analytical Instruments Inc. (USA)
Inventeur(s)
Kuntz, David Micah
Abrégé
Embodiments herein relate to a process for chemical interaction monitoring, such as employing data output from a Raman spectroscopy system relative to a composition undergoing the chemical interaction in a bioreactor. A system can comprise a memory that stores, and a processor that executes, computer executable components. The computer executable components can comprise an identifying component that identifies a raw dataset corresponding to a chemical interaction, and a matching component that generates matched data comprising a set of matches between time series data, corresponding to a range of time over which the chemical interaction was observed, and chemical interaction data comprised by the raw dataset.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Kuntz, David Micah
Abrégé
Embodiments herein relate to a process for chemical interaction monitoring, such as employing data output from a Raman spectroscopy system relative to a composition undergoing the chemical interaction in a bioreactor. A system can comprise a memory that stores, and a processor that executes, computer executable components. The computer executable components can comprise an identifying component that identifies a raw dataset corresponding to a chemical interaction, and a matching component that generates matched data comprising a set of matches between time series data, corresponding to a range of time over which the chemical interaction was observed, and chemical interaction data comprised by the raw dataset.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Kits consisting primarily of reagents and laboratory
equipment, namely, microarrays sold as a unit for scientific
research use. Laboratory equipment, namely, microarrays.
Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation and enhance the separation of the molecule of interest from impurities. Embodiments are described, for example, for the separation of plasma protein(s) from impurities from plasma-derived samples using separation media disclosed herein.
B01J 39/05 - Procédés utilisant des échangeurs organiques sous forme fortement acide
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01J 39/20 - Composés macromoléculaires obtenus par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 47/014 - Procédés d'échange d'ions en généralAppareillage à cet effet dans lesquels les propriétés d’adsorption de l’échangeur d’ions sont utilisées, p. ex. récupération de protéines ou de composés macromoléculaires
Optical configurations for confocal structured illumination Raman (C-SIM Raman) microscopy systems are provided. One example includes a light source configured to project a light; a first set of optical components fixedly aligned along a first optical path, wherein the first set of optical components includes a vortex phase plate and a quarter wave plate, wherein the first set of optical components is configured to receive the light; and a second set of optical components configured to be adjusted between a first operating position and a second operating position. When in the first operating position, the second set of optical component is aligned on the first optical path and is configured to expand, in conjunction with the first set of optical components, the light into an expanded light. When in the second operating position, the second set of optical components is not aligned on the first optical path.
A method can comprise receiving, at a user interface, at least one user selection associated with cell staining. The method can further comprise determining a first product associated with staining a first portion of a cell. The method can further comprise determining, a first simulated view of a stained cell stained with the first product, wherein the first simulated view of the stained cell comprises a portion of the cell highlighted in a particular color. The method can further comprise determining a first emission spectrum associated with the first product and the particular color. The method can further comprise displaying, at the user interface, at least one of the first simulated view of the stained cell and the first emission spectrum associated with the first simulated view of the stained cell.
Systems and methods for calibrating an imager for unmixing of images with multiple fluorophores is described. One example method performed by a computing device includes receiving one or more single-color control images, a foreground channel, and a background channel. The single-color control images are images of the sample with a single fluorophore applied to the sample, and the foreground channel and the background channel are associated with the fluorophore. The method includes determining a difference between the foreground channel and the background channel, acquiring a foreground pixel mask from the difference, averaging a set of foreground pixels in the foreground pixel mask to generate a first spectrum, and subtracting an unstained spectrum from the first spectrum to generate a second spectrum. The second spectrum defines a spectral profile of the sample. A calibration slide and a method of preparing a calibration slide are also described.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
This disclosure generally relates to spatial imaging methods and systems, as well as compositions and kits for use in such methods. Reagents and methods are provided herein that can be used for successful detection using fluorescent and bright-field microscopes and spectral imaging systems of a wide range of various protein markers across numerous types of biological samples.
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
Disclosed herein is a focusing stage. The focusing stage comprises a unit configured to perform real-time focus correction during imaging of a sample. The sample is contained by a substrate positioned on a sample stage. The unit comprises a multi-spot light module comprising a plurality of stationary light sources, at least one optical light splitter configured to reflect a portion of light projected by at least one first stationary light source of the plurality of stationary light sources, at least one detector configured to detect spots of light reflected from interfaces of the substrate, a detector processor configured to calculate, based on locations of the spots on the at least one detector, a correction of a distance between a first objective lens of a plurality of objective lenses and the substrate, and an actuator configured to adjust the distance between the first objective lens and the substrate by the correction.
G02B 7/32 - Systèmes pour la génération automatique de signaux de mise au point utilisant un triangle parallactique avec une ligne de base utilisant des moyens actifs, p. ex. un émetteur de lumière
An instrument for biological analysis includes a base, an excitation source, an optical sensor, an excitation optical system, and an emission optical system. The base is configured to receive a sample holder comprising a plurality of biological samples. The optical sensor is configured to receive emissions from the biological samples in response to the excitation source. The instrument may additionally include a sensor lens enclosed by a lens case and a focusing mechanism including a gear that engages the lens case, the focusing mechanism being accessible outside the enclosure for adjusting a focus. The instrument may further include a sensor aperture dispose along an emission optical path and a blocking structure disposed to cooperate with the sensor aperture such that none of the reflected radiation from an illuminated surface near the sample holder is received by the optical sensor that does not also reflect off another surface of the instrument.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Khadka, Nimesh
Zhang, Lin
England, Jeneffer
Abrégé
A computer-implemented method is provided. The method includes obtaining a chemometric model for one or more levels of parameters associated with composition training data. The composition training data includes data representative of one or more components of a vesicle. Raman spectra data representative of at least one Raman spectrum associated with one or more components of a sample of a vesicle is received. The Raman spectra data representative of the at least one Raman spectrum associated with the one or more components of the sample of the vesicle is transferred into the chemometric model. A level of one or more parameters of the one or more components of the sample of the vesicle is determined based on the chemometric model.
G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
Described herein are compositions, methods, and systems for the detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with QSY2™ probes. Some embodiments relate to detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with a combination of QSY2™ probes and QSY™ probes, or a combination of QSY2™ probes with MGB probes, in a single reaction. Other embodiments relate to detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with a combination of QSY2™ probes with QSY™ probes, or a combination of QSY2™ probes and MGB probes in a single reaction.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
The present invention provides a fluid system for an optical measurement device, comprising a first optical fiber located above and a second optical fiber located below. The fluid system comprises a capillary. The capillary is arranged close to a second fiber core in the radial direction of the second optical fiber, and is configured to be capable of supplying a liquid to a space between the end surface of the first optical fiber and the end surface of the second optical fiber via an end portion of the capillary, such that at least a part of the supplied liquid constitutes at least a part of a liquid column pulled out from the space between the end surface of the first optical fiber and the end surface of the second optical fiber by a surface tension. By means of the fluid system, the liquid can be supplied to the space between the end surfaces of the two optical fibers by means of a simple structure and operation, and particularly, it can be ensured that at least a part of the supplied liquid can be pulled out from the space between the end surfaces of the two optical fibers in the form of the liquid column, greatly improving the liquid supply and detection efficiency and accuracy. In addition, the present invention further provides a fluid operation method for an optical measurement device.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Providing (Saas) software as a service or web-based software
to share project level clinical research data and project
status with clients; clinical trial site selection and trial
site services; clinical trial central lab services; clinical
research and scientific analysis in the field of
pharmaceutical drugs, medical devices, and clinical trials;
clinical trial consulting services; clinical trial cGMP
manufacturing services; clinical trial supply services;
clinical trial management services; clinical trial
laboratory and analytical services; clinical research
services; product development for others relating to
pharmaceuticals, biopharmaceuticals, health care products
and medical products; product testing and research and
industrial research in the field of pharmaceuticals,
biopharmaceuticals, health care products and medical
products; pharmaceutical and biopharmaceutical drug
development services; pharmaceutical and biopharmaceutical
research and development; product development consultation;
product research services, namely, providing analytical
testing, biosafety testing, reporting and laboratory
services for others; scientific and technical product
development and technological consulting and research
services for the pharmaceutical industry; custom design and
development of chemical reagents and biochemical assays;
proving information technology service and technical data
analysis in the field of real-world data and real-world
evidence.
The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.
An illustrative method comprises determining, based on a mass analysis performed by an electron multiplier-based mass analyzer on a first ion population produced from a sample, a mass spectrum comprising one or more peaks representing intensity as a function of mass-to-charge ratio (m/z) of the first ion population across a range of m/z values; determining, based on the mass spectrum, a total ion count of the first ion population and a peak ion count associated with a peak located at a particular m/z value; determining, based on the total ion count of the first ion population and the peak ion count, a total ion count of a second ion population produced from the sample and injected into an image current-based mass analyzer for mass analysis; and setting, based on the total ion count of the second ion population, a calibration parameter for the image current-based mass analyzer.
Systems and methods for enriching or isolating one or more biomolecules are disclosed herein. Existing biomolecule collection and isolation systems and methods require multiple, sequential processes for pre-processing biomolecules after in vitro production and before final isolation (e.g., using affinity chromatography). Failure to sufficiently pre-process (e.g., purify) biomolecules prior to isolation using affinity chromatography frequently results in failure of chromatography equipment, unacceptable decreases in contaminant breakthrough, process throughput, and reagent usage, and increased likelihood of sample contamination or degradation. Systems and methods are disclosed herein that eliminate one or more steps of existing biomolecule isolation systems and protocols, which can decrease time and cost of purification or isolation of biomolecules of interest while maintaining acceptable yield and purity parameters.
A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.
Various embodiments of the present invention comprise a supply chain platform configured in connection with an inventory hub, a supplier system, an enterprise data platform, and a customer system to respond to disturbance events within a supply chain. Such a supply chain platform may collect a variety of supply chain data to determine a risk score relating to such disturbance event(s) and one or more mitigation opportunities in response thereto via a mitigation module configured to generate one or more scenarios and assess the same for efficacy and readiness. Such mitigation opportunities may include the use of one or more supplier sites within the supply chain. Such a supply chain platform may be configured to generate a plurality of reports having interactive data visualizations configurable according to key element data and/or parameter input(s) supplied by a user through a user device and may differentiate between important and inconsequential disturbance events.
A method of operating an analytical instrument comprises ionising a sample to produce sample ions; (i) performing a first ion separation scan by separating sample ions according to a first physico-chemical property, and analysing the separated sample ions by performing one or more MS1 mass analysis scans; and (ii) performing a second ion separation scan by separating sample ions according to the first physico-chemical property, and analysing the separated sample ions by performing a plurality of MS2 mass analysis scans. Each MS2 scan of the plurality of MS2 mass analysis scans uses one MS2 isolation window of a plurality of MS2 isolation windows. The method further comprises analysing MS1 data acquired from the one or more MS1 mass analysis scan(s), and configuring the plurality of MS2 isolation windows based on the analysis of the MS1 data.
Described herein is a tissue sample compartmentalization apparatus and methods for labeling and identifying one or more analytes in a tissue sample. In one embodiment the apparatus comprises a tissue sample compartmentalization module having a first member defining a first plurality of apertures; and a second member defining a second plurality of apertures, the second member pivotably connected to the first member, wherein the second member is configured to engage with the first member to form a closed configuration or pivot relative to the first member between an open configuration and a closed configuration, wherein in the closed configuration the first plurality of apertures and the second plurality of apertures are aligned and form a plurality of sample compartments. The tissue sample compartmentalization module permits individual sample environments for sectioned tissue microscope slides.
Disclosed herein are compositions comprising a solid substrate that is bound to a multivalent cation-binding ligand. The composition can be used to associate positively-charged ion species with the solid substrate so as to facilitate separation methods in the solid phase. Also disclosed are methods of making and using the composition.
Described herein are compositions, methods, and kits for detecting diarrhea causing pathogens from porcine samples. One embodiment described herein is primer pairs and probes for multiplex polymerase chain reaction (PCR) based assays for the detection of diarrhea causing pathogens, such as Rotavirus A, Rotavirus B, and Rotavirus C. Other embodiments include methods and kits for detecting diarrhea causing pathogens.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
The present invention relates to a mixing apparatus for use in a fluid mixing equipment. In an embodiment, the mixing apparatus includes a hub having a top portion and a bottom portion and a central opening extending between the top and bottom portions along a centerline. A base flange extends outward from the bottom portion of the hub and one or more blades are coupled to the hub and extending to a shroud wall surrounding at least a portion of the top portion of the hub.
B01F 27/113 - Agitateurs en forme d'hélice pour produire un écoulement axial, p. ex. en forme d'hélice de navire ou d'avion
B01F 27/213 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation caractérisés par la liaison avec l'entraînement
B01F 101/23 - Mélange d'échantillons de laboratoire, p. ex. en vue d'analyser ou de tester les propriétés des matières
41.
COMPOSITIONS, KITS AND METHODS FOR DIRECT AMPLIFICATION FROM CRUDE BIOLOGICAL SAMPLES
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the detection of target nucleic acids, including those from microbes and/or from infectious agents such as SARS-CoV-2 and other viruses. Embodiments described herein are designed to enable processing and analysis of the sample to detect target nucleic acids within the sample without requiring extraction and/or isolation of nucleic acid from the sample prior to subsequent processing steps. Samples analyzed can thus be “crude” biological samples that do not require pre-processing prior to placement in the workflow.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
42.
MEMBRANE-PENETRATING PEPTIDES TO ENHANCED TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME
The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.
C12N 15/88 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation utilisant la micro-encapsulation, p. ex. utilisant des vésicules liposomiques
A61K 47/54 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique
A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c.-à-d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
Disclosed herein are methods of genotyping a crude nucleic acid sample, the methods including: subjecting the crude nucleic acid sample to a polymerase chain reaction (PCR) mixture directly after obtaining the crude nucleic acid sample from a source; incubating the crude nucleic acid sample in the PCR mixture for a set period of time; performing rapid PCR on the incubated crude nucleic acid sample; and analyzing results from the rapid PCR to determine the nucleic acid sample's genotype. Methods of detecting a disease in a subject are also disclosed.
Systems and methods for Raman spectroscopy. In one example, a method for analysing a sample (404, 530) includes irradiating the sample (404, 530) with a second light and acquiring a fluorescence signal (504), and adjusting a first light (305) based on the fluorescence signal (504). The method includes irradiating the sample (404, 530) with the adjusted first light (305) and acquiring a Raman signal, and analysing a sample composition based on the Raman signal.
The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.
C07K 16/00 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
46.
AMPLIFICATION AND DATA COLLECTION PROTOCOL FOR RAPID GENOTYPING
Disclosed herein are methods of genotyping a crude nucleic acid sample, the methods including: subjecting the crude nucleic acid sample to a polymerase chain reaction (PCR) mixture directly after obtaining the crude nucleic acid sample from a source; performing rapid PCR on the crude nucleic acid sample by subjecting the crude nucleic acid sample to a first plurality of amplification cycles and followed by at least one second amplification cycle; and analyzing results from the rapid PCR to determine the nucleic acid sample's genotype. Each cycle of the first plurality of amplification cycles includes performing denaturation followed by annealing and extension without collecting amplification data. The at least second amplification cycle includes performing denaturation followed by annealing and extension while simultaneously collecting amplification data. Methods of detecting a disease in a subject are also disclosed.
INVITROGEN BIOSERVICES INDIA PRIVATE LIMITED (Inde)
Inventeur(s)
Rystrom, Larry
Ippolito, Kim
Rognin, Nicolas
K Y, Pradeep
Katiki, Naga Harshini
Hamilton, Mary Kristina
Kearns, Austin
Abrégé
Systems and methods for identifying clumps (or groupings) of cells in cell counting systems. One example method executed by an electronic processing device for an image processing system includes receiving an image, captured by an imaging device, of a sample having a plurality of cells. The image includes labeling data for each of the plurality of cells. The method includes applying filters, determined based on the labeling data, to the image, processing the image to identify a plurality of groupings of the plurality of cells, and fitting an ellipse around each of the groupings by determining ellipse data for each of the ellipses. Each respective grouping of the plurality of groupings includes at least two cells having a same viability whose cell membranes are touching at least one other cell in the respective grouping.
Thermo Scientific Portable Analytical Instruments Inc. (USA)
Inventeur(s)
Brand, Audrey
Abrégé
Systems and methods for conducting spectroscopic imaging, such as Raman spectroscopy. One example provides an optical analysis system includes a light source generating an excitation light and an attachment. The attachment includes a reflective surface positioned to reflect light from a sample toward a probe of the optical analysis system. The attachment includes a holder supporting the reflective surface, wherein the sample is positioned between the probe and the reflective surface.
INVITROGEN BIOSERVICES INDIA PRIVATE LIMITED (Inde)
Inventeur(s)
Rystrom, Larry
Ippolito, Kim
Rognin, Nicolas
K Y, Pradeep
Katiki, Naga Harshini
Hamilton, Mary Kristina
Kearns, Austin
Abrégé
Systems and methods for identifying clumps (or groupings) of cells in cell counting systems. One example method executed by an electronic processing device for an image processing system includes receiving an image, captured by an imaging device, of a sample having a plurality of cells. The image includes labeling data for each of the plurality of cells. The method includes applying filters, determined based on the labeling data, to the image, processing the image to identify a plurality of groupings of the plurality of cells, and fitting an ellipse around each of the groupings by determining ellipse data for each of the ellipses. Each respective grouping of the plurality of groupings includes at least two cells having a same viability whose cell membranes are touching at least one other cell in the respective grouping.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Brand, Audrey
Abrégé
Systems and methods for conducting spectroscopic imaging, such as Raman spectroscopy. One example provides an optical analysis system includes a light source generating an excitation light and an attachment. The attachment includes a reflective surface positioned to reflect light from a sample toward a probe of the optical analysis system. The attachment includes a holder supporting the reflective surface, wherein the sample is positioned between the probe and the reflective surface.
G01N 21/85 - Analyse des fluides ou solides granulés en mouvement
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
Disclosed are microfluidic devices. The microfluidic device comprises microfeatures disposed in the loading conduit thereof, configured to separate impurities from a fluid flowing from the loading conduit to the sample compartments. The loading conduit comprises a filtration section comprising the microfeatures, and a narrow section in fluidic communication with the filtration section. The filtration section has a larger cross-sectional area than the cross-sectional area of the narrow section, resulting in added filtration capacity for the loading conduit of the microfluidic device.
Described herein are various embodiments and related methods of a needle and tubing organizing support system that can include one or more of a tray assembly configured to protect and retain a portion of a needle assembly and a securing mechanism. The tray assembly can include a tray body and a capturing recess extending along the tray body for at least partially containing a needle of the needle assembly. The tray assembly can further include a retention element and/or a sheath shaped to extend along a length of the capturing recess and encompass at least a portion of the needle of the needle assembly. The securing mechanism can be coupled to the tray body and configured to releasably secure a tubing of the needle assembly to the tray body.
A61M 5/00 - Dispositifs pour faire pénétrer des agents dans le corps par introduction sous-cutanée, intravasculaire ou intramusculaireAccessoires à cet effet, p. ex. dispositifs de remplissage ou de nettoyage, appuis-bras
A61M 5/14 - Dispositifs de perfusion, p. ex. perfusion par gravitéPerfusion sanguineAccessoires à cet effet
A61M 5/32 - AiguillesParties constitutives des aiguilles relatives au raccordement de celles-ci à la seringue ou au manchonAccessoires pour introduire l'aiguille dans le corps ou l'y maintenirDispositifs pour la protection des aiguilles
A61M 25/02 - Dispositifs de maintien en position, p. ex. sur le corps
A61M 39/08 - TubesMoyens de rangement spécialement adaptés aux tubes
B65D 85/24 - Réceptacles, éléments d'emballage ou paquets spécialement adaptés à des objets ou à des matériaux particuliers pour objets incompressibles ou rigides en forme de baguette ou tubulaires pour aiguilles, clous ou petits objets de forme allongée similaires
A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.
A system for charge detection mass spectrometry (CDMS) performs a process including subdividing an m/z range of interest into a plurality of m/z windows; determining an ion population control parameter for each m/z window; accumulating, in the ion store by one or more accumulation events, a population of ions derived from a sample; transferring the population of ions to a mass analyzer; and mass analyzing the population of ions to acquire a CDMS spectrum of the population of ions. Each accumulation event corresponds to a distinct m/z window of the plurality of m/z windows. The ion population control parameter for each m/z window regulates a quantity of ions accumulated in an ion store during an accumulation event. During each accumulation event, ions within the m/z window corresponding to the accumulation event are accumulated in the ion store based on the ion population control parameter for the corresponding m/z window.
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Dugas, Michael
Abrégé
According to one aspect, a system for identifying an element is described that includes an X-ray source configured to direct x-ray energy to a sample; a detector configured to detect fluorescent emissions from the sample; and a processor configured to: produce an X-ray fluorescent spectrum from the detected fluorescent emissions, where the X-ray fluorescent spectrum is representative of an elemental composition of the sample; calculate a first concentration value for each element in the elemental composition using a first method; select an element from the elemental composition using the first concentration value; and recalculate the concentration value of the selected element using a second method.
G01N 23/223 - Recherche ou analyse des matériaux par l'utilisation de rayonnement [ondes ou particules], p. ex. rayons X ou neutrons, non couvertes par les groupes , ou en mesurant l'émission secondaire de matériaux en irradiant l'échantillon avec des rayons X ou des rayons gamma et en mesurant la fluorescence X
59.
Apparatuses, Systems And Methods For Imaging Flow Cytometry
The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01J 3/44 - Spectrométrie RamanSpectrométrie par diffusion
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
G01N 15/02 - Recherche de la dimension ou de la distribution des dimensions des particules
G01N 15/0227 - Recherche de la dimension ou de la distribution des dimensions des particules par des moyens optiques utilisant l’imagerieRecherche de la dimension ou de la distribution des dimensions des particules par des moyens optiques utilisant l’holographie
G01N 15/10 - Recherche de particules individuelles
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/1404 - Manipulation du flux, p. ex. focalisation hydrodynamique
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
G01N 21/53 - Dispersion, c.-à-d. réflexion diffuse dans un corps ou dans un fluide dans un courant de fluide, p. ex. dans la fumée
Described herein are various embodiments and related methods of a needle and tubing organizing support system that can include one or more of a tray assembly configured to protect and retain a portion of a needle assembly and a securing mechanism. The tray assembly can include a tray body and a capturing recess extending along the tray body for at least partially containing a needle of the needle assembly. The tray assembly can further include a retention element and/or a sheath shaped to extend along a length of the capturing recess and encompass at least a portion of the needle of the needle assembly. The securing mechanism can be coupled to the tray body and configured to releasably secure a tubing of the needle assembly to the tray body.
According to one aspect, a system for identifying an element is described that includes an X-ray source configured to direct x-ray energy to a sample; a detector configured to detect fluorescent emissions from the sample; and a processor configured to: produce an X-ray fluorescent spectrum from the detected fluorescent emissions, where the X-ray fluorescent spectrum is representative of an elemental composition of the sample; calculate a first concentration value for each element in the elemental composition using a first method; select an element from the elemental composition using the first concentration value; and recalculate the concentration value of the selected element using a second method.
G01N 23/223 - Recherche ou analyse des matériaux par l'utilisation de rayonnement [ondes ou particules], p. ex. rayons X ou neutrons, non couvertes par les groupes , ou en mesurant l'émission secondaire de matériaux en irradiant l'échantillon avec des rayons X ou des rayons gamma et en mesurant la fluorescence X
62.
MULTIPLEX PANEL FOR DETECTING GASTROINTESTINAL BACTERIAL NUCLEIC ACIDS
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
Described herein are various embodiments and related methods of a needle and tubing organizing support system that can include one or more of a tray assembly configured to protect and retain a portion of a needle assembly and a securing mechanism. The tray assembly can include a tray body and a capturing recess extending along the tray body for at least partially containing a needle of the needle assembly. The tray assembly can further include a retention element and/or a sheath shaped to extend along a length of the capturing recess and encompass at least a portion of the needle of the needle assembly. The securing mechanism can be coupled to the tray body and configured to releasably secure a tubing of the needle assembly to the tray body.
A61M 5/32 - AiguillesParties constitutives des aiguilles relatives au raccordement de celles-ci à la seringue ou au manchonAccessoires pour introduire l'aiguille dans le corps ou l'y maintenirDispositifs pour la protection des aiguilles
Methods for determining genotypes of structural variants in a sample genome, may include: amplifying nucleic acid sequences at targeted locations in the sample genome by a panel targeting a plurality of structural variant marker to generate sequence reads; mapping the sequence reads to a modified reference genome to produce aligned sequence reads, wherein the modified reference genome includes a wild-type target region and a structural variant target region; for each structural variant marker, determining a read count for a wild-type allele and a read count for a structural variant allele; determining a probability for each possible genotype, wherein the possible genotypes include a homozygous wild-type genotype, a heterozygous genotype and a homozygous structural variant genotype; and selecting the genotype with a maximum probability value to provide an estimated genotype corresponding to the structural variant marker of the sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
A method for determining a genotype of a sample of a polyploid organism, may include: amplifying nucleic acid sequences at targeted locations in a sample genome by a panel targeting a plurality of SNP markers to generate sequence reads; mapping the sequence reads to a reference genome for the polyploid organism; detecting variants in the aligned sequence reads to produce detected variants, wherein the detected variants include detected SNP's corresponding to the SNP markers; determining a probability for each alternate allele dosage of a plurality of possible allele dosages for a corresponding detected SNP, wherein the number of possible allele dosages is equal to a ploidy of the SNP marker plus one; and selecting the alternate allele dosage with a maximum probability value to provide an estimated allele dosage corresponding to the SNP marker, wherein the estimated allele dosage is indicative of the genotype for the SNP marker.
This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.
An x-ray system for measuring a thickness of a material includes a source configured to emit a fan beam of x-ray energy along a beam path. A filter is disposed in the beam path. The filter includes a first thickness positioned in a substantially peripheral region of the fan beam and a second thickness positioned in a substantially central region of the fan beam. The first thickness is different than the second thickness. A detector array includes a plurality of detectors. The detector array is configured to detect the x-ray energy transmitted through the filter. The x-ray energy strikes each detector at an angle associated with the region of the fan beam.
G01B 15/02 - Dispositions pour la mesure caractérisées par l'utilisation d'ondes électromagnétiques ou de radiations de particules, p. ex. par l'utilisation de micro-ondes, de rayons X, de rayons gamma ou d'électrons pour mesurer l'épaisseur
Systems and methods under the present disclosure include sample collection kits, such as for saliva or other fluids. In certain embodiments a detachable funnel can collect saliva from a user. A debris filter can be coupled to the funnel that extends into the test tube. The funnel can comprise or be coupled to a reagent cartridge that, when manipulated by a user, can break open and release reagent into the test tube for use in testing the saliva. Certain embodiments can comprise a casing that can house a test tube and tube cap, with a funnel configured to cover the entire top edge of the casing so as to protect the test tube and tube cap until used by a user.
A61B 10/00 - Instruments pour le prélèvement d'échantillons corporels à des fins de diagnostic Autres procédés ou instruments pour le diagnostic, p. ex. pour le diagnostic de vaccination ou la détermination du sexe ou de la période d'ovulationInstruments pour gratter la gorge
The present disclosure discusses N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure discusses methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for multiplex biological assays.
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
An x-ray system for measuring a thickness of a material includes a source configured to emit a fan beam of x-ray energy along a beam path. A filter is disposed in the beam path. The filter includes a first thickness positioned in a substantially peripheral region of the fan beam and a second thickness positioned in a substantially central region of the fan beam. The first thickness is different than the second thickness. A detector array includes a plurality of detectors. The detector array is configured to detect the x-ray energy transmitted through the filter. The x-ray energy strikes each detector at an angle associated with the region of the fan beam.
G01B 15/02 - Dispositions pour la mesure caractérisées par l'utilisation d'ondes électromagnétiques ou de radiations de particules, p. ex. par l'utilisation de micro-ondes, de rayons X, de rayons gamma ou d'électrons pour mesurer l'épaisseur
G21K 1/10 - Dispositifs de diffusionDispositifs d'absorption
73.
Compression collar for coupling a tube to a tube fitting
Systems, methods, software and computer-usable media for annotating biomolecule-related data are disclosed. In certain exemplified embodiments, the biomolecules can be nucleic acids and the data can be sequence-related data. In various embodiments, systems can include one or more public or private biological attributes (e.g., annotation information databases, data storage devices and systems, etc.) sources, one or more genomic features data sources (e.g., genomic variant tools, genomic variant databases, genomic variant data storage devices and systems, etc.), a computing device (e.g., workstation, server, personal computer, mobile device, etc.) hosting an annotations module and/or a reporting module, and a client terminal.
G06F 16/248 - Présentation des résultats de requêtes
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
A barcode for use in nucleic acid sequencing includes a combinatorial barcode sequence including at least two iterations of a sequence motif, the sequence motif comprising a first nucleotide base from a first group of nucleotide bases, followed by a second nucleotide base from a second group of nucleotide bases, followed by a third nucleotide base from a third group of nucleotide bases. The first group, the second group, and the third group differ from each other; two groups of the first, second, and third groups contain at least two different nucleotide bases; and at least one of the first, second, and third groups comprises at least three different nucleotide bases; and the combinatorial barcode sequence is one of at least eight potential combinatorial barcode sequences exhibiting the plurality of attributes, and the potential combinatorial barcode sequences are synchronized in flow space based on the predetermined order of nucleotide flows.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C40B 20/04 - Identification des éléments d'une bibliothèque au moyen d'une étiquette, d'un marqueur ou d'un autre identificateur lisible ou détectable, p. ex. procédés de décodage
C40B 70/00 - Étiquettes ["tags"] ou marqueurs ["labels"] spécialement adaptés à la chimie combinatoire ou aux chimiothèques, p. ex. "tags" fluorescents ou codes-barres
76.
BARCODE SEQUENCES, AND RELATED SYSTEMS AND METHODS
Methods, system, and kits are provided for sample identification, and, more specifically, for designing, and/or making, and/or using sample discriminating codes or barcodes for identifying sample nucleic acids or other biomolecules or polymers. For example, a plurality of flowspace codewords may be generated, the codewords comprising a string of characters. A location for at least one padding character within the flowspace codewords may be determined. The padding character may be inserted into the flowspace codewords at the determined location. After the inserting, a plurality of the flowspace codewords may be selected based on satisfying a predetermined minimum distance criteria, wherein the selected codewords correspond to valid base space sequences according to a predetermined flow order. And the barcode sequences corresponding to the selected codewords may be manufactured.
A method of performing a digital polymerase chain reaction (dPCR) includes performing an amplification reaction on a reaction mixture including a crude lysate sample to generate amplicons of a target nucleic acid of a pathogen, wherein the crude lysate sample includes biological tissue. Fluorescence is detected from a probe hybridized to the amplicons. A method of detecting pathogen in a subject includes performing the above amplification reaction. The crude lysate sample includes biological tissue from the subject. The fluorescence detected from the probe hybridized to the amplicons is used to determine if the pathogen is present in the subject. Compositions, PCR systems, and kits are also provided.
A method for quality control is executable by an electronic processing device. The method includes receiving a spectrum collected from a sample, inputting the spectrum to an autoencoder trained with a plurality of training spectra belonging to a class, and indicating whether the spectrum is a member of the class based on an output from the trained autoencoder.
A method of performing automatic ion control for mass spectrometry includes acquiring, by charge detection mass spectrometry, a mass spectrum comprising a plurality of peaks representing intensity as a function of mass-to-charge ratio (m/z) of a population of ions analyzed by a mass analyzer during an acquisition event. Based on the mass spectrum, a measured signal density of a selected m/z range of the mass spectrum is determined. An ion population control parameter for a subsequent acquisition event is set based on the measured signal density and a target signal density. The ion population control parameter regulates a population of ions analyzed by the mass analyzer during the subsequent acquisition event.
Systems, methods, and computer readable media with instructions for automated thresholding of dPCR assay data include generating a histogram from emission data collected from reaction sites of a dPCR assay, the histogram comprising a plurality of bins representing a number of reaction sites at different levels of emission signal; based on a cluster of high concentration bins (representing data that is positive or negative for amplification product based on emission signal level), identifying bins having low concentration based on a number of reaction sites per bin being less than or equal to a predetermined amount, and then setting a threshold based on lowest or highest emission signal levels in the low concentration bins.
A method for conjugating oligonucleotides to bead supports includes adding carbodiimide to an aqueous suspension including bread supports having carboxy functional groups; adding hydroxysuccinimide to the aqueous suspension; and adding amine modified oligonucleotide to the aqueous suspension.
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
A method for conjugating oligonucleotides to bead supports includes adding carbodiimide to an aqueous suspension including bread supports having carboxy functional groups; adding hydroxysuccinimide to the aqueous suspension; and adding amine modified oligonucleotide to the aqueous suspension.
A method for conjugating oligonucleotides to bead supports includes adding 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium to an aqueous suspension including bread supports having carboxy functional groups; adding amino-maleimide to the aqueous suspension; and adding diene modified oligonucleotide to the aqueous suspension.
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
A method for enriching clonal populations includes, for a population of target nucleic acids, includes exposing the population of target nucleic acids to a plurality of supports; amplifying the bound target nucleic acids in the presence of a primer to form supports including a plurality of copies of the target nucleic acids of the population of target nucleic acids; applying to the supports a capture primer; applying a magnetic bead functionalize with a moiety to bind to the binder moiety; and separating the first set of supports from the second and third set of supports.
New lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells and tissue. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes and complexes for in vivo delivery of bioactive agents.
A61K 47/69 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament le conjugué étant caractérisé par sa forme physique ou sa forme galénique, p. ex. émulsion, particule, complexe d’inclusion, stent ou kit
C07C 225/20 - Composés contenant des groupes amino et des atomes d'oxygène, liés par des liaisons doubles, liés au même squelette carboné, au moins un des atomes d'oxygène, liés par des liaisons doubles, ne faisant pas partie d'un groupe —CHO, p. ex. aminocétones ayant des groupes amino liés à des atomes de carbone de cycles autres que des cycles aromatiques à six chaînons du squelette carboné
C07D 257/02 - Composés hétérocycliques contenant des cycles comportant quatre atomes d'azote comme uniques hétéro-atomes du cycle non condensés avec d'autres cycles
C07D 295/135 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle substitués par des atomes d'azote liés par des liaisons simples ou doubles avec les atomes d'azote du cycle et les atomes d'azote substituants séparés par des carbocycles ou par des chaînes carbonées interrompues par des carbocycles
89.
VACUUM SYSTEM AND VALVE ASSEMBLY FOR A MASS SPECTROMETER
A vacuum system for a mass spectrometer includes a first vacuum region, a second vacuum region, a vacuum interlock fluidly connected to the vacuum chamber by a first valve, a first pump fluidly connected to the first and second vacuum regions, a second pump fluidly connected to the second vacuum region, and a second valve. The second valve includes a housing, a piston movable within the housing between an evacuation position and an operation position, a first channel connected to a third valve, a second channel connected to the vacuum interlock, a third channel connected to the first pump, and a fourth channel connected to the second vacuum region. In response to the third valve adjusting to a first position, the piston moves to the evacuation position. In response to the third valve adjusting to the second position, the piston moved to the opened position.
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
90.
METHOD FOR AQUEOUS CONJUGATION OF SUBSTRATES USING DIELS-ALDER CHEMISTRY
A method for conjugating oligonucleotides to bead supports includes adding 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium to an aqueous suspension including bread supports having carboxy functional groups; adding amino-maleimide to the aqueous suspension; and adding diene modified oligonucleotide to the aqueous suspension.
A method for enriching clonal populations includes, for a population of target nucleic acids, includes exposing the population of target nucleic acids to a plurality of supports; amplifying the bound target nucleic acids in the presence of a primer to form supports including a plurality of copies of the target nucleic acids of the population of target nucleic acids; applying to the supports a capture primer; applying a magnetic bead functionalize with a moiety to bind to the binder moiety; and separating the first set of supports from the second and third set of supports.
A chromatographic media for separating biopolymers, the chromatographic media having hydrophobic and ionic retention modes, the chromatographic media comprising porous substrate particles including a hydrophobic polymer resin and ion exchange functional groups copolymerized with the hydrophobic polymer resin or grafted to the surface of the porous substrate, wherein the ion exchange functional groups are not greater than about 5 mol% of the porous substrate particles.
B01D 15/32 - Chromatographie en phase liée, p. ex. avec une phase normale liée, une phase inverse ou une interaction hydrophobe
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
B01J 20/28 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation caractérisées par leur forme ou leurs propriétés physiques
B01J 20/285 - Absorbants ou adsorbants poreux à base de polymères
B01J 41/07 - Procédés utilisant des échangeurs organiques sous forme faiblement basique
B01J 41/14 - Composés macromoléculaires obtenus par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 41/20 - Échangeurs d'anions pour procédés chromatographiques
B01J 43/00 - Échange d'ions amphotère, c.-à-d. utilisant des échangeurs d'ions comportant des groupes anioniques et cationiquesUtilisation d'une substance comme échangeur d'ions amphotèreTraitement d'une substance en vue d'améliorer ses propriétés amphotères d'échange d'ions
B01J 47/014 - Procédés d'échange d'ions en généralAppareillage à cet effet dans lesquels les propriétés d’adsorption de l’échangeur d’ions sont utilisées, p. ex. récupération de protéines ou de composés macromoléculaires
Methods and apparatuses of determining sorting efficiency for sorted particles. One method includes acquiring event statistics based on one or more signals generated from interrogating the particles in a fluid stream. The method also includes determining a degree of deviation of the event statistics from reference statistics and taking an automated responsive action in response to the degree of deviation exceeding a threshold level.
G01N 15/1409 - Manipulation des échantillons, p. ex. injection des échantillons
G01N 15/10 - Recherche de particules individuelles
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Embodiments of the invention are disclosed that provide improved computer systems, computerized methods, and computer program products for generating and evaluating automated predictions regarding whether a particular amplification curve from a qPCR assay indicates presence of a target molecule in a sample. In some embodiments, predictions are generated using deep learning networks. In some embodiments, curve-quality predictions are generated and used to assess whether an amplification prediction can be reliably made from a particular amplification curve or whether the curve reflects an anomaly in the qPCR assay. In various embodiments, prediction confidence data is also generated and used, along with prediction data, in an electronic user interface to improve qPCR measurement.
Methods and apparatuses of determining sorting efficiency for sorted particles. One method includes acquiring event statistics based on one or more signals generated from interrogating the particles in a fluid stream. The method also includes determining a degree of deviation of the event statistics from reference statistics and taking an automated responsive action in response to the degree of deviation exceeding a threshold level.
G01N 15/01 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux spécialement adaptée aux cellules biologiques, p. ex. aux cellules sanguines
G01N 15/1404 - Manipulation du flux, p. ex. focalisation hydrodynamique
A61M 1/36 - Autre traitement du sang dans une dérivation du système circulatoire naturel, p. ex. adaptation de la température, irradiation
G01N 15/12 - Recherche de particules individuelles en mesurant des effets électriques ou magnétiques en observant des changements de résistance ou d’impédance à travers des fentes traversées par des particules individuelles, p. ex. en utilisant le principe de Coulter
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/1409 - Manipulation des échantillons, p. ex. injection des échantillons
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 29/22 - Recherche ou analyse des matériaux par l'emploi d'ondes ultrasonores, sonores ou infrasonoresVisualisation de l'intérieur d'objets par transmission d'ondes ultrasonores ou sonores à travers l'objet Détails
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
100.
METHODS AND SYSTEMS FOR ALIGNING AN OPTICAL INSTRUMENT
An optical steering mechanism and methods for using the same. One optical steering mechanism includes a first lens defining a first focal length having a first magnitude and a first polarity and a second lens defining a second focal length having a second magnitude and a second polarity. The first and second magnitudes are substantially equal and the first and second polarities are opposite, and wherein the second lens is positioned to directly receive an optical beam passing through the first lens. The optical beam steering mechanism also includes at least one rotary motor coupled to one of the first lens and the second lens and configured to swing the lens coupled thereto in an arcuate path. An optical beam path of the optical beam passed through the second lens is adjustable by operating the rotary motor.
G02B 21/26 - PlatinesMoyens de réglage pour celles-ci
G02F 1/29 - Dispositifs ou dispositions pour la commande de l'intensité, de la couleur, de la phase, de la polarisation ou de la direction de la lumière arrivant d'une source lumineuse indépendante, p. ex. commutation, ouverture de porte ou modulationOptique non linéaire pour la commande de la position ou de la direction des rayons lumineux, c.-à-d. déflexion
