An assembly for gel electrophoresis includes a gel cassette and a comb. The gel cassette includes a retainer plate and a divider plate coupled to form a cavity therebetween. The comb includes an elongated body having a first end and a second end, an intermediate portion connected to the elongated body and extending between the first and second ends, the intermediate portion having a third end adjacent the first end and a fourth end adjacent the second end, and a plurality of teeth extending from the intermediate portion. The plurality of teeth is spaced apart from at least one of the third end and the fourth end. In response to the comb being received in the gel cassette, the intermediate portion is received in the cavity such that the third and the fourth ends are configured to engage an internal edge of the cavity.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a method of supporting spectroscopic calibration may include: generating a base calibration model using data from multiple base spectroscopic instruments, and finetuning the base calibration model using data from a target spectroscopic instrument to generate a target calibration model for use with the target spectroscopic instrument. In some embodiments, the number of wavelengths used in generating the base calibration model and/or the target calibration model may be less than the total number of wavelengths represented in the output of the spectroscopic instruments.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
5.
MULTIPLEXED ION PRE-SEPARATION FOR MASS SPECTROMETRY
A system includes a pre-separation device for separating precursor ions into a set of distinct fractions of precursor ions based on a physical property of the precursor ions and for sequentially transferring a first subset of distinct fractions of precursor ions included in the set of distinct fractions of precursor ions. The system further includes a mass spectrometer positioned downstream of the pre-separation device for receiving the first subset of distinct fractions of precursor ions. The mass spectrometer includes an ion store for accumulating a first population of product ions produced from each distinct fraction of precursor ions included in the first subset of distinct fractions of precursor ions and a mass analyzer for performing a mass analysis of the first population of product ions.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.
C09K 11/02 - Emploi de substances particulières comme liants, revêtements de particules ou milieux de suspension
C09B 67/00 - Traitements, sans réaction chimique, influençant les propriétés physiques, p. ex. de teintures ou d'impression, des matières colorantes, p. ex. traitement par des solvantsCaractéristiques du procédé de fabrication des préparations tinctorialesPréparations tinctoriales ayant un aspect physique particulier, p. ex. tablettes, feuilles
C09K 11/06 - Substances luminescentes, p. ex. électroluminescentes, chimiluminescentes contenant des substances organiques luminescentes
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G01N 33/533 - Production de composés immunochimiques marqués avec un marqueur fluorescent
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
H04L 12/18 - Dispositions pour la fourniture de services particuliers aux abonnés pour la diffusion ou les conférences
H04L 67/10 - Protocoles dans lesquels une application est distribuée parmi les nœuds du réseau
The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.
The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.
A computer-implemented method for monitoring a biological analysis is provided. The method includes receiving image data of a first portion of a set of reaction sites and determining fluorescence from the image data. The method further includes displaying, on a user interface, a first graphical visualization of determined fluorescence in each reaction site of the first portion and receiving a selection of a subset of reaction sites from the set of reaction sites from a user. The method also includes displaying, on the user interface, in response to the selection, a graphical visualization of the subset of the set of reaction sites, where the graphical visualization of the subset includes an indication of progress of receiving image data in each reaction site of the subset and an indication of determined fluorescence of each reaction site in the subset.
Recombinant nucleic acids, compositions and methods for producing polynucleotides, such as donor sequences, as well as their use in a variety of applications including genome engineering.
C12N 15/64 - Méthodes générales pour la préparation du vecteur, pour son introduction dans la cellule ou pour la sélection de l'hôte contenant le vecteur
A valve assembly for use with an analytical system comprises first (102) and second (104) members, with each member having a through-bore (106, 108) from a first face to a second face. The members are stacked together with their respective first faces in contact to form a seal between them, and one of the members is slidably movable, relative to the other, between at least two configurations: a first configuration in which the through-bores are fluidically connected, and a second configuration in which the through-bores are sealed from each other.
F16K 3/02 - Robinets-vannes ou tiroirs, c.-à-d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation planesGarnitures d'étanchéité à cet effet
F16K 3/08 - Robinets-vannes ou tiroirs, c.-à-d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation planesGarnitures d'étanchéité à cet effet avec éléments de fermeture articulés à pivot en forme de plaques disposées entre l'alimentation et l'évacuation les plaques étant circulaires et pivotant autour de leur centre
F16K 11/074 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout comportant uniquement des tiroirs à éléments de fermeture articulés à pivot à faces d'obturation planes
G01N 30/20 - Injection utilisant une valve d'échantillonnage
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
F16K 3/10 - Robinets-vannes ou tiroirs, c.-à-d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation planesGarnitures d'étanchéité à cet effet avec éléments de fermeture articulés à pivot et dispositions particulières pour tenir écartées les faces d'obturation ou pour les presser l'une contre l'autre
F16K 35/04 - Dispositions empêchant la mise en action accidentelle ou non autorisée offrant une résistance lâche à la commande
21.
HYBRIDIZED ION PRE-SEPARATION FOR MASS SPECTROMETRY
A system includes a first pre-separation device configured to perform a first pre-separation of precursor ions according to mobilities of the precursor ions and a second pre-separation device positioned downstream of the first pre-separation device configured to perform a second pre-separation of precursor ions based on a mass-to-charge ratio (m/z) of the precursor ions. The system further includes a mass spectrometer positioned downstream of the second pre-separation device configured to acquire mass spectra for precursor ions emitted from the second pre-separation device. The second pre-separation device is synchronized with the mass spectrometer such that an m/z range of precursor ions emitted from the second pre-separation device corresponds to a precursor m/z isolation window of the mass spectrometer.
The present invention relates to a needle assembly comprising a needle, a needle housing, wherein the needle housing comprises at least one aligning component. The present invention also relates to a needle receiving assembly comprising a fluid conducting element and a fluid conducting element housing, wherein the fluid conducting element housing comprises at least one aligning component. Additionally, the present invention relates to connection assemblies, samplers and systems that can comprise the needle assembly and the needle receiving assembly.
Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite emission signal data associated with a composite emission signal from at least a first probe type comprising a first label configured to generate a first emission signal and a second probe type comprising a second label configured to generate a second emission signal which has spectrally similar characteristics as said first emission signal. the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite emission signal data, emission signal data associated with a emission signal from a given probe type of the first probe type or the second probe type during the amplification process.
An in vitro method, composition and kit for determining the presence or absence of adenovirus, metapneumovirus, rhinovirus/enterovirus, and parainfluenza in a sample, including providing a reaction mixture containing the sample and at least one primer pair set. The primer pair set includes at least one primer pair A that specifically amplifies a portion of adenovirus genome; at least one primer pair B that specifically amplifies a portion of metapneumovirus genome; at least one primer pair C that specifically amplifies a portion of rhinovirus/enterovirus genome; and at least one primer pair D that specifically amplifies a portion of parainfluenza genome. The reaction mixture is subjected to reaction conditions suitable to amplify targeted nucleic acids, thereby generating at least one amplicon; wherein the presence or absence of at least one amplicon in the sample indicates the presence or absence of adenovirus, metapneumovirus, rhinovirus/enterovirus, and/or parainfluenza in the sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
26.
CAPILLARY EMITTER WITH ELECTROSPRAY IONIZAITON PROVIDING FEMTOLITER TO NANOLITER FLOW RATES
The present disclosure relates to an apparatus and method to achieve electrospray ionization at femtoliter/minute to nanoliter/minute flow rates including relatively rapid alternation between such flow rates within the same device. These flow rates provide enhanced and relatively more uniform ionization of sprayed compounds for subsequent analytical evaluations.
Disclosed are compositions, kits, and methods that enable intra-channel multiplexing by enabling determination of separate detectable signals, each associated with a different assay target, within the same detection channel. The multiple detectable signals can be separately resolved and independently analyzed to enable detection and/or quantification of each respective target. Enabling multiple targets to be assayed within the same detection channel increases the plexy of multiplex assays without relying on additional dyes and concomitant issues of increased spectral overlap.
A system for robotic laboratory operations includes a stationary surface (2) adjacent laboratory equipment and at least one mover (4) configured to perform an action upon a payload atop the stationary surface (2). The action includes but is not limited to translation across at least a portion of the stationary surface. The at least one mover (4) has a drive member (12) configured to drive the translation a carrier that is mounted to the drive member and has a top surface configured to carry a pay load (8). The drive member (12) is configured to drive the at least one mover (4) across the at least the portion of the stationary surface (2) for moving the payload relative to the laboratory equipment.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
32.
Systems, Methods, And Devices For Automated Nucleic Acid And Protein Isolation
Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
33.
CHARACTERIZATION AND OPTIMIZATION OF FLOW CYTOMETRY VOLTAGES
Methods and systems for characterization and optimization of flow cytometry voltages are described herein. According to one aspect of the present disclosure, a method can include applying a plurality of voltages to a detector of a flow cytometry system, the detector optionally comprising a photomultiplier tube (PMT); with the detector, collecting emissions of at least one standard caused by exciting the at least one standard, the at least one standard optionally comprising a bead; measuring a robust coefficient of variance (rCV) for intensity levels of the collected emissions across the plurality of applied voltages; identifying, as an operating voltage setting, a voltage setting where the rCV is essentially asymptotic; and setting the applied voltage of the detector to the identified operating voltage setting.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Chemicals for use in industry and science; diagnostic
preparations for scientific or research use; DNA polymerase,
reagents and reagent kits comprising generic DNA circle, DNA
polymerase and buffers for scientific, medical or veterinary
research use; DNA polymerase, reagents and reagent kits
comprising generic DNA circle, DNA primers, DNA polymerase
and buffers for use in the biotechnology field. Diagnostic preparations for clinical or medical laboratory
use.
36.
OPTIMIZATION OF ACQUISITION WINDOW WIDTH FOR TARGETED MASS SPECTROMETRY
An acquisition schedule for a targeted assay of a sample is generated. The acquisition schedule schedules acquisition, by a mass spectrometer during an acquisition window having a dynamic acquisition window width, of a set of mass spectra for each target analyte included in a set of target analytes included in the sample as the target analytes elute from a separation system. Generating the acquisition schedule includes determining the dynamic acquisition window width based on an acquisition cycle period for the targeted assay. The mass spectrometer is directed to acquire each set of mass spectra in accordance with the acquisition schedule.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Khadka, Nimesh
Pleitt, Kristina
Nolasco Rivera, Michelle
Abrégé
Software and hardware that can be used to perform quality control at various stages of a biopharmaceutical production process, e.g., during upstream or downstream processing. In some examples, the disclosed Raman-based solutions enable real-time or near real-time quantification of protein concentration in various units of the biopharmaceutical production equipment, including but not limited to bioreactors, product holding vessels, and fluid-transfer lines. In some other examples, the disclosed Raman-based solutions enable real-time or near real-time elucidation and monitoring of the secondary structure of the protein, as a quality marker. In at least some examples, the equipment includes an electronic controller configured to perform or initiate an equipment- or process-control action based on the concentration measurements and/or evaluation of the secondary structure.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G16C 20/20 - Identification d’entités moléculaires, de leurs parties ou de compositions chimiques
G16C 20/70 - Apprentissage automatique, exploration de données ou chimiométrie
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. A scientific instrument support apparatus is disclosed comprising generating logic to generate mass spectrum data during a tandem mass tag labeling experiment including a plurality of channels, determining logic to determine, in the generated mass spectrum data, a correction ratio between a first reporter ion peak intensity corresponding to a first non-deuterated tag and a second reporter ion peak intensity corresponding to a first deuterated tag, and normalizing logic to normalize reporter ion intensities corresponding to a second non-deuterated tag and a second deuterated tag based on the determined correction ratio.
m/zm/zm/z, a first correction factor and a second correction factor. The system adjusts, based on the first correction factor, a first observed signal, within the MSn spectrum, for a first reporter ion from the deuterated isobaric mass tag and adjusts, based on the second correction factor, a second observed signal, within the MSn spectrum, for a second reporter ion from the non-deuterated isobaric mass tag.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Khadka, Nimesh
Pleitt, Kristina
Nolasco Rivera, Michelle
Abrégé
Software and hardware that can be used to perform quality control at various stages of a biopharmaceutical production process (100), e.g., during upstream or downstream processing (USP, DSP). In some examples, the disclosed Raman-based solutions enable real-time or near real-time quantification of protein concentration in various units of the biopharmaceutical production equipment, including but not limited to bioreactors, product holding vessels (105), and fluid-transfer lines. In some other examples, the disclosed Raman-based solutions enable real-time or near real-time elucidation and monitoring of the secondary structure of the protein, as a quality marker. In at least some examples, the equipment includes an electronic controller configured to perform or initiate an equipment- or process-control action based on the concentration measurements and/or evaluation of the secondary structure.
A targeted panel with low sample input requirements from a tumor only sample may be processed to estimate mutation load in a tumor sample. The method may include: detecting variants in nucleic acid sequence reads corresponding to targeted locations in the tumor sample genome; annotating detected variants with an annotation information from a population database; filtering the detected variants, wherein the filtering retains the somatic variants and removes germline variants; calculating an initial TMB; and applying a calibration to the initial TMB level to produce a final TMB level for the mutation load of the tumor sample genome. The filtering may also include retaining nonsynonymous SNVs and indels for the analysis.
A pump management system may determine an operating mode for a mass spectrometer; set, based on the operating mode, a pump speed of a turbo pump used to create one or more vacuum stages for the mass spectrometer; and cause the turbo pump to operate at the pump speed while the mass spectrometer operates in accordance with the operating mode. Additionally or alternatively, the pump management system may monitor, using one or more instruments external to the turbo pump, a condition associated with the mass spectrometer while the mass spectrometer operates in accordance with the operating mode; determine, based on the monitoring, that the condition changes by more than a threshold value; and adjust, based on the condition changing by more than the threshold value and while the mass spectrometer is in operation, the pump speed.
A method for correcting abundance ratios between pairs of isobaric reporter ions comprises: (a) measuring, for each liberated reporter-ion moiety the variation, with time, of a signal from said moiety; (b) identifying a first set and a second set of reporter-ion moieties for which the respective signal is, respectively, positively correlated with and not correlated with, the time variation of one or more other signals or variables that pertain to the detection of one or more peptides of interest; (c) for each reporter-ion moiety, decomposing the respective measured mass spectrometric signal into first and second portions that, respectively are and are not attributable to the peptide; (d) for each identified reporter-ion moiety, setting a respective adjusted mass spectrometric signal as being the respective portion of the signal that is attributable to the peptide; and (e) calculating corrected reporter-ion ratios based on the adjusted mass spectrometric signals.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
44.
REAL-TIME CHROMATOGRAPHY DATA FILTER FOR EXPERIMENTS WITH NON-UNIFORM TIMES
Systems and methods taught herein enable improved filtering of chromatography data acquired during a series of scans with non-uniform data sampling intervals (also referred to herein as “scan durations”) by use of a chromatography data filter that operates on a time base that is shorter than any of the data sampling intervals in the series of scans. By employing a filter with such a time base, the systems and methods taught herein improve the signal-to-noise ratio (S/N) of the resulting data and enhance the quality of chromatograms in mass spectrometry real-time signal processing, leading to clearer signals, reduced baseline noise, and smoother peaks in the chromatographic data.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus comprising first logic to generate an electrical signal in a first component of a scientific instrument, wherein the generated electrical signal induces, through capacitive coupling, an electrical response signal in a second component of the scientific instrument, second logic to monitor the electrical response signal induced in the second component, third logic to determine an operational status of the second component based on the monitored electrical response signal, wherein the operational status indicates that the second component is not functioning properly when the monitored electrical response signal is not within a predetermined signal range is disclosed.
Methods for extracting an analyte from a sample through a gas preparation system comprise supplying gas and liquid solvent which solvent can dissolving the analyte in the sample to the sample cell; receiving the liquid solvent containing the analyte from the extraction module to the evaporation container; evaporating the liquid solvent containing the analyte in the evaporation container, wherein the evaporation module is in fluid communication with the sample cell; and starting to perform the evaporating step is allowed after at least a part of the liquid solvent containing the analyte from the extraction module enters the evaporation container. The method integrates extraction and evaporation together to allow “evaporation/concentration online”, thereby avoiding the transfer process of the analyte, saving labor, increasing processing speed, and avoiding the risk of contamination. A gas preparation system for extracting an analyte from a sample is also provided.
A system for executing an experiment. The system includes an instrument electronic device, a processing electronic device, and a workflow electronic device. The workflow electronic device includes an electronic processor. The electronic processor is configured to receive a submission request including an experiment design via a web application, based on the experiment design, generate a request to acquire data, and send, to the instrument electronic device, the request to acquire data. The electronic processor is also configured to, in response to the data being acquired and uploaded to a location by the instrument electronic device according to the request to acquire data, based on the experiment design, generate a request to process acquired data and send, to the processing electronic device, the request to process the acquired data. The electronic processor is further configured to provide, for display via the web application, the acquired data, a processed result, or both.
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
G16H 40/40 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion d’équipement ou de dispositifs médicaux, p. ex. pour planifier la maintenance ou les mises à jour
G06Q 10/06 - Ressources, gestion de tâches, des ressources humaines ou de projetsPlanification d’entreprise ou d’organisationModélisation d’entreprise ou d’organisation
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G06Q 10/0631 - Planification, affectation, distribution ou ordonnancement de ressources d’entreprises ou d’organisations
A system for controlling experiment execution. The system includes an instrument electronic device and a workflow electronic device. The workflow electronic device includes an electronic processor. The electronic processor is configured to receive a submission request via a web application. The submission request includes an experiment design including an acquisition task. The electronic processor is also configured to receive an acquisition rule including pass criteria and an action to take when acquired data does meet not the pass criteria. The electronic processor is further configured to associate the acquisition rule with the acquisition task generate a request to perform the acquisition task, and send, to the instrument electronic device, the request to acquire data. The electronic processor is also configured to determine whether the acquired data meets the pass criteria and, in response to determining the acquired data does not meet the pass criteria, perform the action.
G06Q 10/06 - Ressources, gestion de tâches, des ressources humaines ou de projetsPlanification d’entreprise ou d’organisationModélisation d’entreprise ou d’organisation
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
G06Q 10/0631 - Planification, affectation, distribution ou ordonnancement de ressources d’entreprises ou d’organisations
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
A targeted panel with low sample input requirements from a tumor sample may be processed to identify the presence of a mutational signature. The method may include the steps of: amplifying nucleic acid sequences at targeted locations in the tumor sample genome by a targeted panel to generate nucleic acid sequence reads, detecting variants in the nucleic acid sequence reads, generating a set of trinucleotides by appending flanking 5′ and 3′ bases to each variant, determining a frequency of each trinucleotide to form a mutation matrix, determining a cosine similarity value of the mutation matrix and each mutational signature in a matrix of mutational signatures to form a matrix of similarity values, and selecting mutational signatures from the matrix of mutational signatures when a corresponding cosine similarity value is greater than or equal to a threshold to indicate presence of the selected mutational signatures in the tumor sample genome.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
51.
METHODS FOR ELECTROSCOPIC IMAGING FOR ANALYSIS OF CELLS
Analyzing cells disposed on a sensor array surface of a ChemFET sensor array, may include flowing a solution having a step change in pH across the sensor array surface, wherein ChemFET sensors of the sensor array generate signals in response to the step change in pH to produce electroscopic image data. Multiple frames of the electroscopic image data are acquired during an acquisition time interval. Each frame corresponds to signal samples generated by the sensor array measured at a sampling time during the acquisition time interval. Each frame comprises pixels, wherein a given pixel in the frame corresponds to a signal sample from a given sensor in the sensor array. The electroscopic image data is segmented, based on characteristics of the signal samples, into cell regions corresponding to locations of the cells on the sensor array surface and background regions corresponding to areas on the sensor array having no cells.
Module installation tools and associated methods are disclosed herein. In an example, a module installation tool includes a tool head, a tool base, and an actuator. The module installation tool is configured such that axial motion of the actuator relative to the tool base causes the tool head to rotate relative to the tool base to couple a module to a module receiver or to uncouple the module from the module receiver. In an example, a method includes translating an actuator of a module installation tool in a proximal direction to bring the module into engagement with a module receiver and further translating the actuator in the proximal direction to rotate the tool head relative to the module receiver.
B25B 27/14 - Outils à main ou outillage d'établi, spécialement conçus pour assembler ou séparer des pièces ou des objets, que cela entraîne ou non une certaine déformation, non prévus ailleurs pour assembler des objets autrement que par ajustage à la presse, ou pour les détacher
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A connector is configured to fluidically couple a first conduit and a second conduit to enable flow therethrough of a mobile phase for liquid chromatography. The connector includes an electrically-conductive junction for providing, when the electrically-conductive junction is electrically connected with a power source, an electrospray voltage to the mobile phase. The electrically-conductive junction includes a first receptacle having a first sealing surface that interfaces with the mobile phase and fluidically seals with a distal end of the first conduit, a second receptacle having a second sealing surface that interfaces with the mobile phase and fluidically seals with a proximal end of the second conduit, and a through-hole extending from the first receptacle to the second receptacle. The first sealing surface and the second sealing surface each include an electrochemical corrosion-resistant material.
Methods and systems for detecting gene level copy numbers for BRCA1 and BRCA2 genes include amplifying a nucleic acid sample in a presence of a primer pool to produce a plurality of amplicons. The primer pool may include target-specific primers targeting regions of exons of the BRCA1 and BRCA2 genes and sample ID regions. Overlapping amplicons cover the exons of the BRCA1 and BRCA2 genes. Sample ID amplicons are generated for targeted sample ID regions. The amplicons are sequenced to produce sequence reads. The sequence reads are mapped to a reference genome. Determining whole gene copy numbers for the BRCA1 and BRCA2 genes is based on the number of reads per amplicon for the amplicons associated with the exons of the BRCA1 and BRCA2 genes, respectively, and the number of reads per amplicon for the sample ID amplicons associated with the sample ID regions.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
56.
LINEAR SHAPED INCIDENT SIGNAL FOR RAMAN SPECTROSCOPY
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Brand, Audrey
Abrégé
Systems and methods for obtaining a Raman signal from a sample. One example provides an optical analysis system including a light source generating an excitation light, wherein the excitation light is collimated light having a first shape, an optical component configured to redirect the excitation light as a first light beam, and a focusing component configured to redirect the first light beam as a second light beam. The second light beam interrogates the sample at a predetermined distance from the focusing component in a linear shape.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Berman, Emily
Yasuhara, Ting
Abrégé
Systems and methods for spectroscopic determination of chemical compositions from sample scans. One method includes receiving a first user selection input representative of a user selection of any one or one or more scan modes and receiving a second user selection input representative of a user selection of any one of the one or more target chemical substances to identify. The method also includes receiving scan data generated by a scan of the sample of the unknown chemical composition, and determining a result based on the received scan data generated by the scan of the sample of the unknown chemical composition. The method further includes comparing the result against an expected result for a sample scan associated with the selected target chemical substance and generating instructions for displaying indicia representative of a primary compound associated with the selected target chemical substance.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Berman, Emily
Yasuhara, Ting
Abrégé
Systems and methods for spectroscopic determination of chemical compositions from sample scans. One method includes receiving a first user selection input representative of a user selection of any one or one or more scan modes and receiving a second user selection input representative of a user selection of any one of the one or more target chemical substances to identify. The method also includes receiving scan data generated by a scan of the sample of the unknown chemical composition, and determining a result based on the received scan data generated by the scan of the sample of the unknown chemical composition. The method further includes comparing the result against an expected result for a sample scan associated with the selected target chemical substance and generating instructions for displaying indicia representative of a primary compound associated with the selected target chemical substance.
Spectrum series are generated based on acquired photons responsive to irradiating a location of a sample with multiple light pulses. The spectrum series is processed with a trained 2D neural network to generate Raman spectrum with reduced fluorescence.
Thermo Scientific Portable Analytical Instruments Inc. (USA)
Inventeur(s)
Brand, Audrey
Abrégé
Systems and methods for obtaining a Raman signal from a sample. One example provides an optical analysis system including a light source generating an excitation light, wherein the excitation light is collimated light having a first shape, an optical component configured to redirect the excitation light as a first light beam, and a focusing component configured to redirect the first light beam as a second light beam. The second light beam interrogates the sample at a predetermined distance from the focusing component in a linear shape.
A system for fluid handling is provided. The system includes a housing, and a cap assembly connected to a fluid reservoir, a sliding secure mechanism connected to the housing, and an optical sensor configured to detect if the cap assembly is fully inserted. The cap assembly is configured to fluidically seal the fluid reservoir, and to be removeable from the fluid reservoir. The sliding secure mechanism is configured to receive the cap assembly. The sliding secure mechanism includes a tactile detent to provide mechanical feedback in response to the cap assembly being fully inserted.
Multiple droplet streams are produced with shaped apertures that are situated at distal ends of flow members. The droplet streams interact with and are desolvated by a shear gas flow. A variable number of droplet streams at fixed locations can be produced by selection of a suitable extraction electric field.
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/6825 - Détecteurs faisant intervenir la détection d’acides nucléiques
A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
G16B 20/40 - Génétique de populationDéséquilibre de liaison
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 30/10 - Alignement de séquenceRecherche d’homologie
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
G16B 40/10 - Traitement du signal, p. ex. de spectrométrie de masse ou de réaction en chaîne par polymérase
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
H03M 7/30 - CompressionExpansionÉlimination de données inutiles, p. ex. réduction de redondance
Provided are laser sources, the laser sources comprising at least one diode; and an optic fiber of a predefined length disposed between the laser source and a position for a target such that the optic fiber communicates light pulses from the laser source as a source light to the position for the target, wherein the position is illuminated by the source light so as to reduce speckles in a captured image of the target. Also provided are methods for providing source light for generating an image, comprising: generating illumination with one or more laser diodes; and passing the illumination through an optic fiber having a plurality of bends therein such that source light is emitted from the optic fiber so as to illuminate a target with the source light, the source light reducing speckles in an image of the target.
Various methods are disclosed for amplifying nucleic acid sequences in a nucleic acid sample. The methods involve forming at least five amplification reaction mixes each including an aliquot from a sample source that includes nucleic acid sequences, using at least five different assays each including a pair of amplification primers, the assays selected from the group of assays in Table 1 and/or targeting the sequences specified in Table 1.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
67.
TRINUCLEOTIDE CAP ANALOGS, PREPARATION AND USES THEREOF
This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.
A61K 47/54 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
A pipette tip holder includes a tray including an array of openings to receive pipette tips. A plurality of the openings provide access to interiors of a plurality of enclosures. At least one opening of the array of openings of the array is free of an enclosure. The pipette tip holder further includes a container. The tray is secured over a mouth of the container. The at least one opening of the array of openings provides access through the tray to the interior of the container.
Systems or techniques are provided for facilitating retrieval augmented generative question and answer boosting. In various embodiments, a system can access a plain text question regarding a scientific instrument. In various aspects, the system can generate, via a large language model that references a document-graph repository, a structured or unstructured answer for the plain text question. In various instances, the document-graph repository can comprise a plurality of document-graphs that respectively correspond to a plurality of technical documents. In various cases, for a first document-graph that corresponds to a first technical document, leaf nodes of the first document-graph can represent respective text blocks written in the first technical document, and non-leaf nodes of the first document-graph can respectively represent a document title, one or more section headings, and one or more scientific instrument identifiers written in the first technical document and beneath which the respective text blocks are nested.
Apparatus, methods, and systems including a syringe for delivering a fluid. The syringe includes a syringe body, a piston, an insert, and a removable plunger. The piston is located in a lumen of the syringe body such that a bottom of the piston together with interior walls of the syringe define a working volume. The piston comprising a cavity. The insert is located at a desired position in the lumen. The insert narrowing the lumen and being configured to prevent retraction of the piston beyond the desired position. The removable plunger is configured to removably couple to the piston. The removable plunger includes a tip configured to couple with the cavity of the piston and move the piston when a force is applied at the plunger, and to decouple from the piston when the piston engages the insert at the desired position.
The present disclosure relates to N-protected NH-rhodanine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays.
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C09B 11/24 - Phtaléines contenant des groupes amine
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
77.
Systems and Methods for Performing Data-Dependent Tandem Mass Spectrometry
An illustrative method of performing data-dependent tandem mass spectrometry includes a mass spectrometer acquiring an MS1 mass spectrum of ions produced from a sample, determining, based on the MS1 mass spectrum, a set of observed precursor ions, determining expected fragment ions for the set of observed precursor ions, and acquiring one or more MS2 mass spectra for select ions observed in the MS1 mass spectrum, wherein the expected fragment ions are excluded from being selected for MS2 analysis.
Sample preparation compositions and methods for purifying plasmid DNA from biological samples is provided, are provided. The compositions and methods provided herein allow pDNA analysis to be carried out without centrifugation. The preparation process is amenable to high throughput processing using manual or robotic platforms.
Described herein are compositions, methods, and kits for detecting antibiotic resistance genes in a sample, such as a wound swab. One embodiment described herein is primer pairs and probes for individual or multiplex polymerase chain reaction (PCR) based assays for the detection of one or more antibiotic resistance gene targets comprising resistance to molecularly characterized extended-spectrum β-lactamases (MESBLs); extended-spectrum β-lactamases (ESBLs); carbapenemase; AmpC β-lactamase; β-lactamase; lincosamide, macrolide, streptogramin; trimethoprim; macrolides; methicillin; colistin; sulfonamide; tetracycline; vancomycin; nitroimidazole; quinolone; or aminoglycoside.
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
Thermo Scientific Portable Analytical Instruments Inc. (USA)
Inventeur(s)
Kuntz, David Micah
Abrégé
Embodiments herein relate to a process for chemical interaction monitoring, such as employing data output from a Raman spectroscopy system relative to a composition undergoing the chemical interaction in a bioreactor. A system can comprise a memory that stores, and a processor that executes, computer executable components. The computer executable components can comprise an identifying component that identifies a raw dataset corresponding to a chemical interaction, and a matching component that generates matched data comprising a set of matches between time series data, corresponding to a range of time over which the chemical interaction was observed, and chemical interaction data comprised by the raw dataset.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Kuntz, David Micah
Abrégé
Embodiments herein relate to a process for chemical interaction monitoring, such as employing data output from a Raman spectroscopy system relative to a composition undergoing the chemical interaction in a bioreactor. A system can comprise a memory that stores, and a processor that executes, computer executable components. The computer executable components can comprise an identifying component that identifies a raw dataset corresponding to a chemical interaction, and a matching component that generates matched data comprising a set of matches between time series data, corresponding to a range of time over which the chemical interaction was observed, and chemical interaction data comprised by the raw dataset.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Kits consisting primarily of reagents and laboratory
equipment, namely, microarrays sold as a unit for scientific
research use. Laboratory equipment, namely, microarrays.
Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation and enhance the separation of the molecule of interest from impurities. Embodiments are described, for example, for the separation of plasma protein(s) from impurities from plasma-derived samples using separation media disclosed herein.
B01J 39/05 - Procédés utilisant des échangeurs organiques sous forme fortement acide
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01J 39/20 - Composés macromoléculaires obtenus par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 47/014 - Procédés d'échange d'ions en généralAppareillage à cet effet dans lesquels les propriétés d’adsorption de l’échangeur d’ions sont utilisées, p. ex. récupération de protéines ou de composés macromoléculaires
Optical configurations for confocal structured illumination Raman (C-SIM Raman) microscopy systems are provided. One example includes a light source configured to project a light; a first set of optical components fixedly aligned along a first optical path, wherein the first set of optical components includes a vortex phase plate and a quarter wave plate, wherein the first set of optical components is configured to receive the light; and a second set of optical components configured to be adjusted between a first operating position and a second operating position. When in the first operating position, the second set of optical component is aligned on the first optical path and is configured to expand, in conjunction with the first set of optical components, the light into an expanded light. When in the second operating position, the second set of optical components is not aligned on the first optical path.
A method can comprise receiving, at a user interface, at least one user selection associated with cell staining. The method can further comprise determining a first product associated with staining a first portion of a cell. The method can further comprise determining, a first simulated view of a stained cell stained with the first product, wherein the first simulated view of the stained cell comprises a portion of the cell highlighted in a particular color. The method can further comprise determining a first emission spectrum associated with the first product and the particular color. The method can further comprise displaying, at the user interface, at least one of the first simulated view of the stained cell and the first emission spectrum associated with the first simulated view of the stained cell.
Systems and methods for calibrating an imager for unmixing of images with multiple fluorophores is described. One example method performed by a computing device includes receiving one or more single-color control images, a foreground channel, and a background channel. The single-color control images are images of the sample with a single fluorophore applied to the sample, and the foreground channel and the background channel are associated with the fluorophore. The method includes determining a difference between the foreground channel and the background channel, acquiring a foreground pixel mask from the difference, averaging a set of foreground pixels in the foreground pixel mask to generate a first spectrum, and subtracting an unstained spectrum from the first spectrum to generate a second spectrum. The second spectrum defines a spectral profile of the sample. A calibration slide and a method of preparing a calibration slide are also described.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
This disclosure generally relates to spatial imaging methods and systems, as well as compositions and kits for use in such methods. Reagents and methods are provided herein that can be used for successful detection using fluorescent and bright-field microscopes and spectral imaging systems of a wide range of various protein markers across numerous types of biological samples.
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
Disclosed herein is a focusing stage. The focusing stage comprises a unit configured to perform real-time focus correction during imaging of a sample. The sample is contained by a substrate positioned on a sample stage. The unit comprises a multi-spot light module comprising a plurality of stationary light sources, at least one optical light splitter configured to reflect a portion of light projected by at least one first stationary light source of the plurality of stationary light sources, at least one detector configured to detect spots of light reflected from interfaces of the substrate, a detector processor configured to calculate, based on locations of the spots on the at least one detector, a correction of a distance between a first objective lens of a plurality of objective lenses and the substrate, and an actuator configured to adjust the distance between the first objective lens and the substrate by the correction.
G02B 7/32 - Systèmes pour la génération automatique de signaux de mise au point utilisant un triangle parallactique avec une ligne de base utilisant des moyens actifs, p. ex. un émetteur de lumière
An instrument for biological analysis includes a base, an excitation source, an optical sensor, an excitation optical system, and an emission optical system. The base is configured to receive a sample holder comprising a plurality of biological samples. The optical sensor is configured to receive emissions from the biological samples in response to the excitation source. The instrument may additionally include a sensor lens enclosed by a lens case and a focusing mechanism including a gear that engages the lens case, the focusing mechanism being accessible outside the enclosure for adjusting a focus. The instrument may further include a sensor aperture dispose along an emission optical path and a blocking structure disposed to cooperate with the sensor aperture such that none of the reflected radiation from an illuminated surface near the sample holder is received by the optical sensor that does not also reflect off another surface of the instrument.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventeur(s)
Khadka, Nimesh
Zhang, Lin
England, Jeneffer
Abrégé
A computer-implemented method is provided. The method includes obtaining a chemometric model for one or more levels of parameters associated with composition training data. The composition training data includes data representative of one or more components of a vesicle. Raman spectra data representative of at least one Raman spectrum associated with one or more components of a sample of a vesicle is received. The Raman spectra data representative of the at least one Raman spectrum associated with the one or more components of the sample of the vesicle is transferred into the chemometric model. A level of one or more parameters of the one or more components of the sample of the vesicle is determined based on the chemometric model.
G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
Described herein are compositions, methods, and systems for the detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with QSY2™ probes. Some embodiments relate to detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with a combination of QSY2™ probes and QSY™ probes, or a combination of QSY2™ probes with MGB probes, in a single reaction. Other embodiments relate to detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with a combination of QSY2™ probes with QSY™ probes, or a combination of QSY2™ probes and MGB probes in a single reaction.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
The present invention provides a fluid system for an optical measurement device, comprising a first optical fiber located above and a second optical fiber located below. The fluid system comprises a capillary. The capillary is arranged close to a second fiber core in the radial direction of the second optical fiber, and is configured to be capable of supplying a liquid to a space between the end surface of the first optical fiber and the end surface of the second optical fiber via an end portion of the capillary, such that at least a part of the supplied liquid constitutes at least a part of a liquid column pulled out from the space between the end surface of the first optical fiber and the end surface of the second optical fiber by a surface tension. By means of the fluid system, the liquid can be supplied to the space between the end surfaces of the two optical fibers by means of a simple structure and operation, and particularly, it can be ensured that at least a part of the supplied liquid can be pulled out from the space between the end surfaces of the two optical fibers in the form of the liquid column, greatly improving the liquid supply and detection efficiency and accuracy. In addition, the present invention further provides a fluid operation method for an optical measurement device.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Providing (Saas) software as a service or web-based software
to share project level clinical research data and project
status with clients; clinical trial site selection and trial
site services; clinical trial central lab services; clinical
research and scientific analysis in the field of
pharmaceutical drugs, medical devices, and clinical trials;
clinical trial consulting services; clinical trial cGMP
manufacturing services; clinical trial supply services;
clinical trial management services; clinical trial
laboratory and analytical services; clinical research
services; product development for others relating to
pharmaceuticals, biopharmaceuticals, health care products
and medical products; product testing and research and
industrial research in the field of pharmaceuticals,
biopharmaceuticals, health care products and medical
products; pharmaceutical and biopharmaceutical drug
development services; pharmaceutical and biopharmaceutical
research and development; product development consultation;
product research services, namely, providing analytical
testing, biosafety testing, reporting and laboratory
services for others; scientific and technical product
development and technological consulting and research
services for the pharmaceutical industry; custom design and
development of chemical reagents and biochemical assays;
proving information technology service and technical data
analysis in the field of real-world data and real-world
evidence.
The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.
An illustrative method comprises determining, based on a mass analysis performed by an electron multiplier-based mass analyzer on a first ion population produced from a sample, a mass spectrum comprising one or more peaks representing intensity as a function of mass-to-charge ratio (m/z) of the first ion population across a range of m/z values; determining, based on the mass spectrum, a total ion count of the first ion population and a peak ion count associated with a peak located at a particular m/z value; determining, based on the total ion count of the first ion population and the peak ion count, a total ion count of a second ion population produced from the sample and injected into an image current-based mass analyzer for mass analysis; and setting, based on the total ion count of the second ion population, a calibration parameter for the image current-based mass analyzer.
Systems and methods for enriching or isolating one or more biomolecules are disclosed herein. Existing biomolecule collection and isolation systems and methods require multiple, sequential processes for pre-processing biomolecules after in vitro production and before final isolation (e.g., using affinity chromatography). Failure to sufficiently pre-process (e.g., purify) biomolecules prior to isolation using affinity chromatography frequently results in failure of chromatography equipment, unacceptable decreases in contaminant breakthrough, process throughput, and reagent usage, and increased likelihood of sample contamination or degradation. Systems and methods are disclosed herein that eliminate one or more steps of existing biomolecule isolation systems and protocols, which can decrease time and cost of purification or isolation of biomolecules of interest while maintaining acceptable yield and purity parameters.
