An artificial neural network (ANN) receives signal measurements, based on data generated by a nucleic acid sequencing instrument, at an input layer. The ANN is applied to the signal measurements and produces signal correction values and predicted ideal signal measurements. The signal correction values may be subtracted from the signal measurements to form corrected signal measurements. The predicted ideal signal measurements may be used by a base caller to produce a sequence of base calls. The corrected signal measurements may be used by a variant caller for variant calling. The ANN may comprise a convolutional neural network (CNN). The CNN may have a U-NET architecture that includes an encoder and a decoder. The U-NET may include a Convolutional Block Attention Module (CBAM). The CBAM may applied to the outputs of a last pooling layer of the encoder and provides refined feature maps to a first layer of the decoder.
Thermo Scientific Portable Analytical Instruments Inc. (USA)
Inventor
Maltas, Rafet
Zhang, Lin
Abstract
Systems/techniques are provided for facilitating unary differentiation of compositions. In various embodiments, a system can comprise a processor that can execute computer-executable components stored in a non-transitory computer-readable memory. In various instances, the computer-executable components can comprise a conversion component that generates one or more binary features based on a set of transformed peaks of a sample spectrum, the sample spectrum associated with a sample of interest. In various cases, the computer-executable components can further comprise an identification component that that determines a probability parameter for the sample of interest based on the one or more binary features, wherein the probability parameter is associated with a first target composition.
The present invention relates to a method for operating a cooling system of a laboratory device, wherein the cooling system (4) comprises a compressor assembly, comprising at least a first compressor (30), a cooling device (32), an expansion device (34), and an evaporator (36), which are fluidly connected to form a main cycle, and wherein the cooling system further comprises a refrigerant circulating through the main cycle. The method comprises operating the compressor assembly based on a compressor control loop for controlling a compressor controlled variable xc(t) based on a compressor reference variable wc(t) and a compressor feedback variable rc(t), and the method further comprises operating the expansion device based on a superheat control loop for controlling a superheat controlled variable xs(t) based on a superheat reference variable ws(t) and a superheat feedback variable rs(t).
Various embodiments for improved dPCR systems and methods are disclosed. Some embodiments provide a multi-instrument dPCR system that facilitates analyzing samples together across multiple arrays of partitions, multiple sample plates, and/or multiple instruments to provide a better and higher throughput dPCR system. Aspects of various embodiments provide one or more of the following: Correction of illumination bias; quality control processing; flexible grouping of samples across partitions, sample plates, and instruments for thresholding and analysis; inter-instrument signal equalization for each dye channel to facilitate grouping samples across instruments; and improved auto-thresholding.
The present disclosure provides systems and devices for gel electrophoresis including electrophoresis clamp assemblies also known as electrophoresis cores. Methods for electrophoresis using the systems and devices of the disclosure are also provided.
An artificial neural network (ANN) receives signal measurements, based on data generated by a nucleic acid sequencing instrument, at an input layer. The ANN is applied to the signal measurements and produces signal correction values and predicted ideal signal measurements. The signal correction values may be subtracted from the signal measurements to form corrected signal measurements. The predicted ideal signal measurements may be used by a base caller to produce a sequence of base calls. The corrected signal measurements may be used by a variant caller for variant calling. The ANN may comprise a convolutional neural network (CNN). The CNN may have a U-NET architecture that includes an encoder and a decoder. The U-NET may include a Convolutional Block Attention Module (CBAM). The CBAM may applied to the outputs of a last pooling layer of the encoder and provides refined feature maps to a first layer of the decoder.
Methods and systems for automatically loading samples to an analytical instrument are disclosed. The method includes positioning a first pedestal and a second pedestal of the analytical instrument at a loading position, extending a first nozzle towards a sample space between the two pedestals, releasing a first sample from the first nozzle to the sample space so that the first sample is in direct contact with both the first surface and the second surface, and retracting the first nozzle away from the first sample held between the first surface and the second surface.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventor
Maltas, Rafet
Zhang, Lin
Abstract
Systems/techniques are provided for facilitating unary differentiation of compositions. In various embodiments, a system can comprise a processor that can execute computer-executable components stored in a non-transitory computer-readable memory. In various instances, the computer-executable components can comprise a conversion component that generates one or more binary features based on a set of transformed peaks of a sample spectrum, the sample spectrum associated with a sample of interest. In various cases, the computer-executable components can further comprise an identification component that that determines a probability parameter for the sample of interest based on the one or more binary features, wherein the probability parameter is associated with a first target composition.
The present invention relates to a method for operating a cooling system of a laboratory device, wherein the cooling system (4) comprises a compressor assembly, comprising at least a first compressor (30), a cooling device (32), an expansion device (34), and an evaporator (36), which are fluidly connected to form a main cycle, and wherein the cooling system further comprises a refrigerant circulating through the main cycle. The method comprises operating the compressor assembly based on a compressor control loop for controlling a compressor controlled variable xc(t) based on a compressor reference variable wc(t) and a compressor feedback variable rc(t), and the method further comprises operating the expansion device based on a superheat control loop for controlling a superheat controlled variable xs(t) based on a superheat reference variable ws(t) and a superheat feedback variable rs(t).
Disclosed embodiments include a digital Polymerase chain reaction (dPCR) operative system having a method of digital image processing that corrects for illumination bias in data relating to an array of a sample plate comprising one or more arrays of sample partitions. In some embodiments, the method includes obtaining background image data corresponding to a digital image captured by the camera of the dPCR system after a background cycle of a dPCR assay. With respect to each partition, a local flat-fielding coefficient is derived and applied to the background image data to obtain local-bias-corrected background image data. Next, the local bias-corrected background data is used to determine a set of global flat-fielding coefficients. The local and global flat-fielding coefficients are subsequently used to correct illumination bias endpoint image data to generate endpoint bias-corrected illumination values.
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
G06V 10/98 - Detection or correction of errors, e.g. by rescanning the pattern or by human interventionEvaluation of the quality of the acquired patterns
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Kits consisting primarily of chemical reagents and also including laboratory equipment, namely, microarrays, sold as a unit for scientific research use
Methods for performing signal collection on fluorescence emitted by an object to be subjected to detection in a sample comprise: performing photoelectric detection on fluorescence emitted by the object to generate an electrical signal; integrating the electrical signal; collecting the integrated electrical signal; and in response to a trigger indication, releasing, within a time period, more than 90% of charges accumulated in a charge integrator that integrates the electrical signal, wherein the time period is less than 15% of a full width at half maximum of a time-domain waveform corresponding to fluorescence intensity. By releasing most of the accumulated charges in a timely manner within a short time during the process of integrating an electrical signal generated during fluorescence detection, the problem of the dynamic range of a fluorescence detector being insufficient is effectively solved, such that the dynamic range can be expanded.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Competent cells for gene transfer used in non-clinical
molecular biology and microbiology research and accompanying
multi-well array plates sold as a unit (term considered too
vague by the International Bureau pursuant to Rule 13 (2)
(b) of the Regulations).
09 - Scientific and electric apparatus and instruments
Goods & Services
Downloadable computer software for optimizing chemical compounds; downloadable desktop computer software for compound optimization for use in scientific research and laboratory analysis.
Methods for determining an arm aneuploidy score in a tumor sample genome include selectively amplifying nucleic acid sequences at specific locations in the tumor genome using a targeted panel to generate sequence reads. Next, divide the genome locations into segments with homogeneous copy numbers based on log odds of heterozygous SNPs and CNV log ratios of the sequence reads. Identify gain and loss segments relative to a reference copy number and intersecting respective chromosome arms. Compare the cellularities of these segments to a minimum threshold. Retain the longest segment for the arm that meets the minimum cellularity and sum its total bases. Divide this total by the number of bases in the arm to yield a fraction. If this fraction meets a minimum threshold, filter the segment based on fold changes and determine gains or losses. Count the arms with called gains or losses to generate the arm aneuploidy score.
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
The invention relates to a method for reducing germ density in the useful space of a device, comprising heating the useful space to a process temperature such that a relative humidity in the useful space is below an upper moisture limit; controlling the relative humidity in the useful space to a first target value for a first duration, wherein a humidification system introduces an active ingredient; deactivating the control of the relative humidity; and reducing the active ingredient in the useful space for a reduction duration. The invention also relates to a corresponding device and to a corresponding system.
39 - Transport, packaging, storage and travel services
Goods & Services
Transportation and delivery of goods; local delivery of
laboratory equipment, laboratory supplies, chemicals,
biochemicals and diagnostic kits; distribution services,
namely, delivery to medical facilities of investigational
medicinal products, comparator medicinal products and
placebos used in clinical trials; freight transportation
services; packaging of goods for transportation purposes;
transport of valuables; warehousing and storage of goods;
parcel delivery services; delivery of mail-order goods.
25.
NON-DISRUPTIVE AND SHAPE-UNCONSTRAINED LEAK DETECTION IN LIQUID CHROMATOGRAPHY
Systems/techniques are provided for facilitating non-disruptive and shape-unconstrained leak detection in liquid chromatography. In various embodiments, a system can capture, via at least one camera, at least one timeseries of images depicting an electrospray emitter of a chromatograph. In various aspects, the system, can determine a leak rate afflicting the chromatograph based on: executing a droplet-volume-prediction neural network on respective images of the at least one timeseries of images; executing a droplet-pixel-prediction neural network on respective images of the at least one timeseries of images; or executing a leak-rate-prediction neural network on an entirety of the at least one timeseries of images.
An illustrative ion trap comprises a first group of ring-shaped electrodes that are centered around an ion trap central axis and that define a first annular trapping volume, and a second group of ring-shaped electrodes that are centered around the ion trap central axis and that define a second annular trapping volume. Upon receipt of radio frequency (RF) voltages, the first group of ring-shaped electrodes and the second group of ring-shaped electrodes generate a first pseudo-potential barrier and a second pseudo-potential barrier. The first pseudo-potential barrier is configured to prevent the ions from escaping the first annular trapping volume and the second annular trapping volume in an axial direction through an exit. The second pseudo-potential barrier is positioned between the first annular trapping volume and the second annular trapping volume. The second pseudo-potential barrier is weaker than the first pseudo-potential barrier.
An illustrative ion trap comprises a first group of ring-shaped electrodes that are centered around an ion trap central axis and that define a first annular trapping volume, and a second group of ring-shaped electrodes that are centered around the ion trap central axis and that define a second annular trapping volume. Upon receipt of radio frequency (RF) voltages, the first group of ring-shaped electrodes and the second group of ring-shaped electrodes generate a first pseudo-potential barrier and a second pseudo-potential barrier. The first pseudo-potential barrier is configured to prevent the ions from escaping the first annular trapping volume and the second annular trapping volume in an axial direction through an exit. The second pseudo-potential barrier is positioned between the first annular trapping volume and the second annular trapping volume. The second pseudo-potential barrier is weaker than the first pseudo-potential barrier.
39 - Transport, packaging, storage and travel services
Goods & Services
Transportation and delivery of goods; local delivery of
laboratory equipment, laboratory supplies, chemicals,
biochemicals and diagnostic kits; distribution services,
namely, delivery to medical facilities of investigational
medicinal products, comparator medicinal products and
placebos used in clinical trials; freight transportation
services; packaging of goods for transportation purposes;
transport of valuables; warehousing and storage of goods;
parcel delivery services; delivery of mail-order goods.
Described herein are various methods and embodiments of a fluid processing system and foam breaker devices for at least reducing foam formation in a container of the fluid processing system. In some embodiments, the foam breaker device includes a mounting hub extending along a longitudinal axis of the foam breaker device. The mounting hub can include a coupling feature for coupling the foam breaker device to a drive shaft of the fluid processing system. The foam breaker device can also include a wall structure extending circumferentially from the mounting hub at an angle relative to the longitudinal axis of the foam breaker device. The wall structure can cause a reduction in the foam volume as a result of at least a part of the wall structure contacting the foam volume.
A system to measure the temperature of a sample using two measurements of the sample's conductivity is described herein. The system includes a first surface, a second surface, and an electronic processing unit. The second surface is positioned opposite the first surface for holding the sample between the first surface and the second surface by surface tension. The electronic processing unit is configured to apply at least one electrical signal across the sample via a first electrode and a second electrode, determine, based on the at least one electrical signal applied across the sample, a first conductivity of the sample at a first frequency associated with the at least one electrical signal and a second conductivity of the sample at a second frequency associated with the at least one electrical signal, and determine, based on the first conductivity and the second conductivity, a temperature of the sample.
An ion guide includes a series of electrodes disposed between first and second ends of the ion guide. A power source is configured to simultaneously apply voltages that generate a first electric force that urges ions to migrate towards the second end and DC voltages configured to create a DC electric field that generates a second electric force that urges the ions to migrate towards the first end. A controller is configured to determine a target m/z value associated with the ions and set, based on the target m/z value, a magnitude of the DC voltages.
The invention relates to a method for reducing a germ density in the usable space of a device, comprising heating the usable space to a process temperature such that the relative humidity in the usable space is below an upper humidity limit value; regulating the relative humidity in the usable space to a first target value for a first duration, wherein a humidification system introduces an active substance; deactivating the relative humidity regulation; and degrading the active substance in the usable space for a degradation duration. The invention also relates to a corresponding device and to a corresponding system.
A61L 9/14 - Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances
B01D 53/00 - Separation of gases or vapoursRecovering vapours of volatile solvents from gasesChemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols
F24F 1/0087 - Indoor units, e.g. fan coil units with humidification means
F24F 1/009 - Indoor units, e.g. fan coil units characterised by heating arrangements
The present disclosure provides freeze-dried eukaryotic cell culture media, feeds, and supplement formulations that have enhanced stability when stored refrigerated or at room temperature. The present disclosure contemplates freeze-dried eukaryotic cell culture media and supplement compositions having reduced moisture content and enhanced stability when compared to non-freeze-dried eukaryotic cell culture media, feed, and supplement compositions. Additional aspects include methods of freeze-drying eukaryotic cell culture media and supplement compositions to enhance stability. Further encompassed are methods for stabilizing eukaryotic cell culture media, feed, or supplement compositions by freeze-drying. Still further aspects include freeze-dried eukaryotic cell culture media, feed, and supplement compositions that are agglomerated or powered media compositions.
A chromatography system includes a first chromatography column and a panel having a top face and an opposing bottom face, a first cavity being formed on the panel so as to pass through the top face and be encircled by an inner surface. The panel bounds an inlet fluid channel having an end terminating at an inlet opening formed on the inner surface encircling the first cavity so that the inlet fluid channel communicates with the first cavity. The panel also bounds a plurality of first outlet fluid channels each having an end terminating at an outlet opening formed on the inner surface encircling the first cavity so that each of the plurality of first outlet fluid channels communicate with the first cavity, a first one of the plurality of first outlet fluid channels being in fluid communication with the first chromatography column. A first valve is rotatably disposed within the first cavity.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
F16K 11/085 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only taps or cocks with cylindrical plug
An illustrative a spectroscopic system includes a source for generating an electromagnetic radiation, a detector for detecting a light emitted from a sample, and a programmable wavelength filter including multiple pixels for selectively directing the light emitted from the sample to the detector. The programmable wavelength filter is set to a first setting and the sample is irradiated with the electromagnetic radiation such that the programmable wavelength filter directs a first portion of the light emitted from the sample to the detector. At least one pixel region of the programmable wavelength filter includes a first non-zero number of pixels directing the light from the sample to the detector and a second non-zero number of pixels not directing the light from the sample to the detector such that the detector detects the first portion of the light.
Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.
Methods and systems for ion trap type resonant collision induced dissociation (CID) on labile compounds to drive selective dissociation along desired dissociation pathways are disclosed. Specifically, the present disclosure includes, systems, methods, and computer readable media (CRM) configured to introduce ions of at least one labile compound with multiple dissociation pathways into a trapping area of a dissociation cell. The dissociation cell is configured to generate a substantially quadrupolar radio frequency electrostatic confinement potential in the ion trapping region. The kinetic energy of selected precursor ions is increased while a selective dissociation parameter is maintained to cause the precursor ions to selectively dissociate primarily to first-generation products without being ejected from the trapping area. The selective dissociation parameter is determined in part by the Mathieu q value and controls the maximum kinetic energy or velocity of the ions.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Non-ionic surfactants for use in scientific research, laboratory processes, industrial processes, and manufacturing processes; detergents for use in scientific research, laboratory processes, industrial processes, and manufacturing processes; chemical reagents for use in scientific research, product development, and manufacturing processes, other than for medical or veterinary purposes
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Kits consisting primarily of chemical reagents and also including laboratory equipment, namely, microarrays, sold as a unit for scientific research use
45.
SYSTEM FOR INSTRUMENT OPTIMIZATION USING ANALYTE BASED MASS SPECTROMETER AND ALGORITHM PARAMETERS
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, an apparatus, includes a sample introduction device and mass spectrometer. The mass spectrometer includes first logic to receive a sample from a queue, the sample comprising an analyte; second logic to determine instrument methods and data analysis parameters based on a nature of the analyte; and third logic to process the sample by applying the instrument methods and data analysis parameters. WO
A workflow for detecting and/or quantifying a target protein and target nucleic acid(s) in a biological sample, includes binding of the target protein using a pair of proximity probes; synthesizing a nucleic acid extended product including the target protein using a polymerase; and thermocycling a reaction mixture including nucleic acid extended product and the target nucleic acid(s) to form amplicons of the nucleic acid extended product and the target nucleic acid(s); and detecting an emission signal from the amplicons of the nucleic acid extended product and of the target nucleic acid(s) using polymerase chain reaction (PCR). A first emission signal being used to determine a particle titer, and a second emission signal, being used to determine a genome titer. The method may be used for determining the ratios of capsids with full genomes, partially filled capsids and empty capsids, e.g. AAV capsids.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
A device, apparatus, and method for performing an auto-focusing search by generating, while a well is positioned at a first position, a first calibration image showing a fiducial of the well and generating, while the well is positioned at a second position, a second calibration image showing the fiducial, selecting, for a second auto-focusing search, the first position instead of the second position based at least in part on the first calibration image showing a better image focus for the fiducial relative to the second calibration image and performing the second auto-focusing search in a positional region defined based on the first position. The second auto-focusing search performed by generating, while the well is positioned at a third position within the positional region, a third calibration image showing the fiducial, and determining a target position for the well and determining a genetic property of a sample contained in the well.
A method for separation of analyzing oligonucleotides or nucleic acid biopolymers includes loading a sample containing a plurality of oligonucleotide species onto a column; flowing a mobile phase consisting of a combination of a first solution and a second solution through the column to elute the plurality of oligonucleotide species; and analyzing at least one of the first oligonucleotide species and the second oligonucleotide species using a mass spectrometer. The plurality of oligonucleotide species includes a first oligonucleotide species and a second oligonucleotide species. The first solution includes an ion pairing reagent. The ion pairing reagent includes a primary amine or a secondary amine. The proportion of the first solution and the second solution in the mobile phase is varied over time to separate the first oligonucleotide species from the second oligonucleotide species.
Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SIR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.
C07F 7/08 - Compounds having one or more C—Si linkages
C07F 9/6561 - Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
C12Q 1/6816 - Hybridisation assays characterised by the detection means
Compositions for detecting the presence of monkeypox virus in a biological sample includes a monkeypox nucleic acid forward primer; a monkeypox nucleic acid reverse primer; and a monkeypox nucleic acid probe. Methods include a multiplex assay including a three-channel panel with a single assay per channel, that includes a monkeypox assay with a reporter and a quencher, a non-variola orthopox assay with a reporter and a quencher, and a RNase P assay with a reporter and a quencher. A kit for detecting monkeypox virus nucleic acid includes the assay components including the forward and reverser primers and probes for each of the three assays in the multiplex assay.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
H03M 7/30 - CompressionExpansionSuppression of unnecessary data, e.g. redundancy reduction
53.
DATA STORAGE FOR SCALABLE PROCESSING OF LARGE FILES GENERATED BY SCIENTIFIC INSTRUMENTS
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a support apparatus for a scientific instrument comprises first, second, and third logics. The first logic is configured to acquire a first data file via detectors of the instrument. The second logic is configured to convert the first data file into a plurality of second data files stored in an object storage. Each of the second files is named using a suitable file naming convention. The third logic is configured to process a request for a data portion of the first data file and to provide the data portion by accessing one or more of a first memory cache, a second memory cache, and the object storage to obtain a corresponding portion of a corresponding one of the second data files identified based on the file naming convention.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a chromatography instrument support apparatus may include: first logic to receive a chromatogram data set; second logic to determine one or more peak locations for the chromatogram data set by processing the chromatogram data set through a machine-learning computational model to determine a plurality of input parameter values for a Gaussian curve fitting the chromatogram data set; and third logic to provide the one or more peak locations for further small molecule Liquid-Chromatography Mass Spectrometry (LC/MS) processing.
A mass spectrometry method comprises: identifying groups of charge state distributions (CSDs) within deconvoluted mass spectrometric data, wherein CSDs of each group comprise at least one common mass spectral peak that is assigned a different respective charge state within each CSD of each group; assigning, within each group, a respective weighting factor to each CSD; calculating, within each group and using the weighting factors, a score-weighted average molecular weight for each compound CSD; locating, within each group, a single target CSD that that corresponds to a molecular weight that is closest to the calculated average; for each common peak of each identified group, summing the intensities of the respective common assigned mass spectral peak across the group and assigning the summed intensity to the single target CSD of the group; discarding all CSDs other than the target CSDs; and calculating an abundance of each component compound using the summed intensities.
A spectrophotometer, a cleaning unit, and a method for cleaning a spectrophotometer. The spectrophotometer comprises: a measurement unit, which comprises a pair of detection components; a cleaning unit, which comprises an injection mechanism and a discharge mechanism; and a closed cleaning chamber, wherein walls for bounding the cleaning chamber can be formed by the pair of detection components and the cleaning unit, and the pair of detection components are cleaned in the cleaning chamber. The discharge mechanism is configured to discharge a cleaning agent from the cleaning chamber when the injection mechanism injects the cleaning agent into the cleaning chamber. Therefore, the risk of leakage of the cleaning agent from the cleaning chamber can be reduced or avoided.
B08B 15/00 - Preventing escape of dirt or fumes from the area where they are producedCollecting or removing dirt or fumes from that area
B08B 3/04 - Cleaning involving contact with liquid
B08B 5/04 - Cleaning by suction, with or without auxiliary action
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
39 - Transport, packaging, storage and travel services
Goods & Services
Transportation and delivery of goods; local delivery of laboratory equipment, laboratory supplies, chemicals, biochemicals and diagnostic kits; distribution services, namely, delivery to medical facilities of investigational medicinal products, comparator medicinal products and placebos used in clinical trials; freight transportation services; packaging of goods for transportation purposes; transport of valuables; warehousing and storage of goods; parcel delivery services; delivery of mail-order goods.
39 - Transport, packaging, storage and travel services
Goods & Services
Transportation and delivery of goods; local delivery of laboratory equipment, laboratory supplies, chemicals, biochemicals and diagnostic kits; distribution services, namely, delivery to medical facilities of investigational medicinal products, comparator medicinal products and placebos used in clinical trials; freight transportation services; packaging of goods for transportation purposes; transport of valuables; warehousing and storage of goods; parcel delivery services; delivery of mail-order goods.
Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.
A fluidic device for performing isotachophoresis comprise a sub-strate defining a fluidic network comprising a plurality of inlet channels, the outlets of the inlet channels meeting at a juncture, an intermediate channel extending from the juncture and fluidically coupled to the outlets of the plurality of inlet channels, a first reservoir at the juncture and fluidically coupled to the inlet channels and the intermediate channel, a second reservoir fluidically coupled to the intermediate channel at a location downstream of the juncture, wherein the inlet channels, the collection channel, and the intermediate channel are co-planar, and wherein the first reservoir and second reservoir are configured to produce substantially equal pressure heads. Methods of loading the fluidic device for isotachophoresis can be sequentially through the various inlet channels.
Disclosed are systems and methods for improving the function of analytical instruments used to analyze dye-labelled nucleic acid samples by minimizing spectral anomalies from dye data. A computer system communicatively coupled to the instrument is configured to select multiple different nucleic acids of different sizes and determine dye spectral profiles associated with each of the different nucleic acids. The spectral profiles are used to generate multiple dye matrices each respectively associated with different nucleic acid sizes. When analyzing a test sample, the dye matrices are then separately applied to nucleic acid fragments with sizes similar to the nucleic acids from which the dye matrices were derived, better tailoring the dye matrices to the conditions in which they were generated and minimizing unwanted dye data artifacts.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
67.
NON-IONIC SURFACTANTS AND METHODS OF MAKING AND USING THE SAME
Disclosed here are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
68.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
Disclosed herein are aspects of a compound according to Formula I. The compounds are useful as surfactants and can be used in applications for which a surfactant is indicated. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are aspects of a method for making the compounds and methods for using the compounds in cell assays.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
70.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
The present disclosure provides surfactant-based compositions, methods, kits, and systems for use in cell permeabilization, analytical and diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. In certain aspects, permeabilization buffers comprising defined concentrations of nonionic surfactants in polar protic solvents permeabilize diverse cell types while preserving intracellular targets and compatibility with downstream detection chemistry and nucleic acid amplification. Related compositions include loading, blocking, running, and mountant buffers, as well as polypeptide stabilization and viral inactivation solutions formulated with the surfactants. In additional aspects, the surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows, including determination of encapsulation efficiency. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes of buffer performance and recommend protocol parameters.
An optical cell density sensor is provided. The optical cell density sensor comprising: a light source arranged to emit light in a first direction for passing through a flow of fluid with suspended cells; a first detector substantially aligned with the first direction, arranged to detect attenuated light from the light source and generate an attenuated light signal; a second detector substantially offset from the first direction, arranged to detect scattered light from the light source and generate a scattered light signal; and a processor configured to calculate cell density based on the attenuated light signal and the scattered light signal.
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
G01N 15/075 - Investigating concentration of particle suspensions by optical means
G01N 21/53 - Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke
G01N 15/06 - Investigating concentration of particle suspensions
72.
NON-IONIC SURFACTANTS AND METHODS OF MAKING AND USING THE SAME
Disclosed herein are aspects of a compound according to Formula (I). tHe compounds are useful as surfactants and can be used in applications for which a surfactant is indicated. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are aspects of a method for making the compounds and methods for using the compounds in cell assays.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/44 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
74.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/44 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
75.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded and can be used as replacements for current surfactants, such as octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Surfactant-based compositions, methods, kits, and systems can be used in cell permeabilization, analytical/diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. Permeabilization buffers comprising the compounds, permeabilize diverse cell types while preserving intracellular targets and are compatible with a variety of downstream detection and analysis methods. Compositions include loading, blocking, running, mountant buffers, polypeptide stabilization and viral inactivation solutions. The surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes performance and recommend protocol parameters.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
76.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
The present disclosure provides surfactant-based compositions, methods, kits, and systems for use in cell permeabilization, analytical and diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. In certain aspects, permeabilization buffers comprising defined concentrations of nonionic surfactants in polar protic solvents permeabilize diverse cell types while preserving intracellular targets and compatibility with downstream detection chemistry and nucleic acid amplification. Related compositions include loading, blocking, running, and mountant buffers, as well as polypeptide stabilization and viral inactivation solutions formulated with the surfactants. In additional aspects, the surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows, including determination of encapsulation efficiency. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes of buffer performance and recommend protocol parameters.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/44 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
77.
COMPOSITION, SYSTEM, AND METHOD FOR ADENO-ASSOCIATED VIRUS (AAV) PRODUCTION
Compositions, systems, kits and methods for producing AAV vectors in a suspension at high viral titer and purity. Certain aspects include one or more of media, cells, transfection reagent, viral production enhancer, host cell DNA encapsidation reducer, and lysis buffer, each of which is designed to provide maximal viral particle production with high purity from culture of eukaryotic cells, such as 293 cells and insect cells.
A loading assembly for a capping and decapping apparatus, comprising: a frame mechanism, which can be driven to move in a first direction relative to a bit assembly of an apparatus; a loading mechanism, which is movably accommodated in the frame mechanism and provided with first containers or second containers of different structures; and a guide element, which has a guide surface and is fixedly arranged at a base of the apparatus. When the frame mechanism moves in the first direction, the loading mechanism can be in contact with the guide element and move relative to the bit assembly from a first position to a second position, wherein the loading mechanism can move along the guide surface relative to the frame mechanism in a second direction perpendicular to the first direction. When the loading mechanism is in the first position, a first part of bits can cap and decap the first containers, and when the loading mechanism is in the second position, a second part of the bits can cap and decap the second containers. The loading assembly enables the same bit assembly of the same capping and decapping apparatus to cap and decap different containers, thereby broadening the range of application of the capping and decapping apparatus.
Disclosed herein are aspects of a compound according to Formula I
Disclosed herein are aspects of a compound according to Formula I
Disclosed herein are aspects of a compound according to Formula I
The compounds are useful as surfactants and can be used in applications for which a surfactant is indicated. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are aspects of a method for making the compounds and methods for using the compounds in cell assays.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C09K 23/42 - Ethers, e.g. polyglycol ethers of alcohols or phenols
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/48 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms
C08G 65/334 - Polymers modified by chemical after-treatment with organic compounds containing sulfur
The present invention relates to an optical device, for use in ion chromatography detection of a sample, comprising: a light source portion configured to provide light having a predetermined wavelength range to form a first light beam propagating in free space; and a flow cell provided with a guide channel, wherein the guide channel accommodates the sample and is provided with a first lens and a second lens, a first clear aperture is provided at the first lens, and a second clear aperture is provided at the second lens; the ratio of the first clear aperture to the second clear aperture is between 1 and 3, and the ratio of the equivalent spot size of a focusing portion to the second clear aperture is not greater than 0.5, such that the ratio of the light intensity of a third light beam to the light intensity of the first light beam is not less than 62%. By generating a highly focused light beam in the flow cell, the device increases the ratio of the intensity of emitted light to the intensity of incident light, thereby improving the light utilization efficiency and enhancing the sample detection sensitivity. In addition, the present invention also relates to an analysis device and a data analysis method.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
A control method for a sample moving device, and a sample moving device, which can achieve calibration of a robotic arm with low cost and with a simple structure. The control method comprises: an acquisition step: by means of a camera, acquiring an image containing both a current position of a first marker and a current position of a second marker; a determination step: on the basis of position related information between the first marker and the second marker in the image, and relative position information between the first marker and the second marker when the first marker is at a target position, determining a difference between the current position of the first marker and the target position, and, on the basis of the difference, determining the amount of movement to be performed by the first marker; a first movement step: on the basis of said amount of movement, controlling a robotic arm so as to move the first marker from the current position to an updated position; and a determination step: determining whether a difference between the updated position and the target position is less than or equal to a preset threshold, and if the difference is greater than the threshold, executing an acquisition step.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Providing temporary use of online non-downloadable artificial intelligence (AI) software for discovering, analyzing, generating, organizing, and applying scientific knowledge from scientific literature, protocols, institutional knowledge, and community insights; providing a scientific research workspace featuring online non-downloadable software for experiment design, protocol planning, reagent selection, and management of scientific research workflows.
86.
SYSTEMS AND METHODS FOR PREDICTING ELUTION TIMES FOR TARGETED MASS SPECTROMETRY
A system for predicting elution times for targeted mass spectrometry determines elution times for a first set of analytes eluting from a separation system. The system accesses metadata associated with analytes included in a second set of analytes, the second set of analytes including at least one analyte not included in the first set of analytes. The system predicts, based on the metadata associated with the analytes included in the second set of analytes and the elution times for the first set of analytes, elution times for the second set of analytes. The system sets, based on the predicted elution times for the second set of analytes, a targeted acquisition schedule for the second set of analytes. The system directs a mass spectrometer to acquire, based on the targeted acquisition schedule, mass spectra of product ions derived from the second set of analytes.
Systems and methods for autofocus using artificial intelligence include (i) capturing a plurality of monochrome images over a nominal focus range, (ii) identifying one or more connected components within each monochrome image, (iii) sorting the identified connected components based on a number of pixels associated with each connected component, (iv) generating a focus quality estimate of at least a portion of the sorted connected components using a machine learning module, and (iv) calculating a target focus position based on the focus quality estimate of the evaluated connected components. The calculated target focus position can be used to perform cell counting using artificial intelligence, such as by (i) generating a seed likelihood image and a whole cell likelihood image based on output—a convolutional neural network and (ii) generating a mask indicative quantity and/or pixel locations of objects based on the seed likelihood image.
Systems or techniques are provided for spectral analysis. In various embodiments, a system can comprise a memory that stores computer executable instructions and a processor that executes the computer executable instructions. The computer executable instructions can comprise a compression component that compresses the one or more unknown spectra, using an encoder, into one or more compressed unknown spectra in a latent space; and a search component that matches the one or more compressed unknown spectra with one or more compressed known spectra to obtain one or more candidate matches of the sample.
G01N 21/35 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
89.
Systems And Methods Of Error-Tolerant Real-Time Searching
Disclosed herein is a method of analyzing a sample by mass spectrometry. A precursor is acquired from the sample. At least one mass spectrum associated with the precursor is generated based on performing a first level of analysis on the precursor. At least one motif in the precursor is identified based on performing a real-time error-tolerant search of the at least one mass spectrum using stored mass spectral reference data. Based on identifying the at least one motif in the precursor, at least one second level of analysis on the at least one motif is initiated to determine an identity of the precursor.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Providing temporary use of non-downloadable software for tracking, monitoring, and managing product traceability information, lot-specific product data, certificates of analysis, certificates of conformance, certificates of quality, certificates of sterility, and product change notifications for laboratory, scientific, biotechnology, pharmaceutical, diagnostic, and industrial products; providing online databases featuring quality assurance and product compliance information
Methods of using levels PD-L1 expression by circulating cancer cells in the screening, monitoring, treatment and diagnosis of cancer in subjects are disclosed, along with methods of monitoring probability of progression free survival and/or overall survival of such subjects. The methods are based on assaying the level of PD-L1 expression in one or more of circulating tumor cells (CTCs), epithelial to mesenchymal transition CTCs (EMTCTCs), cancer associated macrophage-like cells (CAMLs), and cancer associated vascular endothelial cells (CAVEs) isolated from a subject having cancer, where the subject is undergoing immunotherapy treatment.
Thermo Fisher Scientific India Private Limited (India)
Thermo Electron Scientific Instruments LLC (USA)
Inventor
Bhat, Pramod Ramachandra
Raina, Rajesh
Piratla, Anant
Nesargi, Sumeet
Abstract
Systems and methods for managing a platform link. One example provides a scientific instrument support system includes a server storing a trained language model and an instrument computing device having a display device configured to provide a user interface, a memory including a platform link application providing an interface between the instrument computing device and a platform of network-based services, a transceiver, and an electronic processor communicatively connected to the display device, the memory, and the transceiver. The electronic processor is configured to receive a request associated with the platform link application, transmit, with the transceiver, the request to the trained language model, receive, with the transceiver and from the trained language model, a recommended command associated with the request, confirm an access level of a user submitting the request, and in response to confirming the access level of the user, execute the recommended command.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
95.
PARALLEL PROCESSING AND HORIZONTAL SCALING FOR PEAK DETECTION
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus may include: first logic to receive, from a mass spectrometer, injections data for each of a plurality of injections associated with a sample; second logic to determine peak data for each of the plurality of injections by executing, in parallel on a node, a peak detection algorithm for each of the plurality of injections, third logic to collate the peak data for each of the plurality of injections; and fourth logic to provide the collated peak data for further processing.
Systems/techniques are provided for facilitating post-detection chromatographic peak validation. In various embodiments, a system can cause a chromatography device coupled to a mass spectrometer to scan a specimen, thereby yielding a chromatogram and mass spectra. In various aspects, the system can identify, via a peak detection algorithm, a plurality of purported peaks in the chromatogram. In various instances, the system can separate, via execution of a machine learning classifier on respective ones of the plurality of purported peaks, the plurality of purported peaks into a set of valid peaks and a set of invalid peaks. In various cases, the system can perform a mass spectrometry analysis on first portions of the mass spectra that correspond to the set of valid peaks but not on second portions of the mass spectra that correspond to the set of invalid peaks.
A method for biological sample analysis includes receiving reference nucleic acid sequence information and biological sample nucleic acid sequence data, comparing the biological sample nucleic acid sequence data to the reference nucleic acid sequence information, outputting overall consensus metrics of the biological sample nucleic acid sequence data and the reference nucleic acid sequence information based on the comparison and outputting one or more region level consensus metrics of one or more regions of each of the biological sample nucleic acid sequence data and the reference nucleic acid sequence information.
Oxford University Innovation Limited (United Kingdom)
Thermo Fisher Scientific Inc. (USA)
OMASS Therapeutics (United Kingdom)
Inventor
Robinson, Carol
Lutomski, Corinne
El-Baba, Tarick
Liko, Idlir
Syka, John
Hinkle, Josh
Abstract
The disclosure concerns a method of analysing a sample comprising an intact membrane protein, in a sample comprising non-synthetic biological membrane material. The method involves irradiating a gas phase proto membrane protein ion with infrared irradiation from a laser source.
THERMO FISHER ELECTRON SCIENTIFIC INSTRUMENTS LLC (a Delaware corporation) (USA)
LIFE TECHNOLOGIES CORPORATION (a Delaware corporation) (USA)
Inventor
Irwin, Julian James
Smith, Wesley Glen
Abstract
Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.
