42 - Scientific, technological and industrial services, research and design
Goods & Services
Providing (Saas) software as a service or web-based software
to share project level clinical research data and project
status with clients; clinical trial site selection and trial
site services; clinical trial central lab services; clinical
research and scientific analysis in the field of
pharmaceutical drugs, medical devices, and clinical trials;
clinical trial consulting services; clinical trial cGMP
manufacturing services; clinical trial supply services;
clinical trial management services; clinical trial
laboratory and analytical services; clinical research
services; product development for others relating to
pharmaceuticals, biopharmaceuticals, health care products
and medical products; product testing and research and
industrial research in the field of pharmaceuticals,
biopharmaceuticals, health care products and medical
products; pharmaceutical and biopharmaceutical drug
development services; pharmaceutical and biopharmaceutical
research and development; product development consultation;
product research services, namely, providing analytical
testing, biosafety testing, reporting and laboratory
services for others; scientific and technical product
development and technological consulting and research
services for the pharmaceutical industry; custom design and
development of chemical reagents and biochemical assays;
proving information technology service and technical data
analysis in the field of real-world data and real-world
evidence.
2.
Calibration of an Image Current-based Mass Analyzer Included in a Mass Spectrometer
An illustrative method comprises determining, based on a mass analysis performed by an electron multiplier-based mass analyzer on a first ion population produced from a sample, a mass spectrum comprising one or more peaks representing intensity as a function of mass-to-charge ratio (m/z) of the first ion population across a range of m/z values; determining, based on the mass spectrum, a total ion count of the first ion population and a peak ion count associated with a peak located at a particular m/z value; determining, based on the total ion count of the first ion population and the peak ion count, a total ion count of a second ion population produced from the sample and injected into an image current-based mass analyzer for mass analysis; and setting, based on the total ion count of the second ion population, a calibration parameter for the image current-based mass analyzer.
Systems and methods for enriching or isolating one or more biomolecules are disclosed herein. Existing biomolecule collection and isolation systems and methods require multiple, sequential processes for pre-processing biomolecules after in vitro production and before final isolation (e.g., using affinity chromatography). Failure to sufficiently pre-process (e.g., purify) biomolecules prior to isolation using affinity chromatography frequently results in failure of chromatography equipment, unacceptable decreases in contaminant breakthrough, process throughput, and reagent usage, and increased likelihood of sample contamination or degradation. Systems and methods are disclosed herein that eliminate one or more steps of existing biomolecule isolation systems and protocols, which can decrease time and cost of purification or isolation of biomolecules of interest while maintaining acceptable yield and purity parameters.
A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.
Various embodiments of the present invention comprise a supply chain platform configured in connection with an inventory hub, a supplier system, an enterprise data platform, and a customer system to respond to disturbance events within a supply chain. Such a supply chain platform may collect a variety of supply chain data to determine a risk score relating to such disturbance event(s) and one or more mitigation opportunities in response thereto via a mitigation module configured to generate one or more scenarios and assess the same for efficacy and readiness. Such mitigation opportunities may include the use of one or more supplier sites within the supply chain. Such a supply chain platform may be configured to generate a plurality of reports having interactive data visualizations configurable according to key element data and/or parameter input(s) supplied by a user through a user device and may differentiate between important and inconsequential disturbance events.
A method of operating an analytical instrument comprises ionising a sample to produce sample ions; (i) performing a first ion separation scan by separating sample ions according to a first physico-chemical property, and analysing the separated sample ions by performing one or more MS1 mass analysis scans; and (ii) performing a second ion separation scan by separating sample ions according to the first physico-chemical property, and analysing the separated sample ions by performing a plurality of MS2 mass analysis scans. Each MS2 scan of the plurality of MS2 mass analysis scans uses one MS2 isolation window of a plurality of MS2 isolation windows. The method further comprises analysing MS1 data acquired from the one or more MS1 mass analysis scan(s), and configuring the plurality of MS2 isolation windows based on the analysis of the MS1 data.
Described herein is a tissue sample compartmentalization apparatus and methods for labeling and identifying one or more analytes in a tissue sample. In one embodiment the apparatus comprises a tissue sample compartmentalization module having a first member defining a first plurality of apertures; and a second member defining a second plurality of apertures, the second member pivotably connected to the first member, wherein the second member is configured to engage with the first member to form a closed configuration or pivot relative to the first member between an open configuration and a closed configuration, wherein in the closed configuration the first plurality of apertures and the second plurality of apertures are aligned and form a plurality of sample compartments. The tissue sample compartmentalization module permits individual sample environments for sectioned tissue microscope slides.
Disclosed herein are compositions comprising a solid substrate that is bound to a multivalent cation-binding ligand. The composition can be used to associate positively-charged ion species with the solid substrate so as to facilitate separation methods in the solid phase. Also disclosed are methods of making and using the composition.
Described herein are compositions, methods, and kits for detecting diarrhea causing pathogens from porcine samples. One embodiment described herein is primer pairs and probes for multiplex polymerase chain reaction (PCR) based assays for the detection of diarrhea causing pathogens, such as Rotavirus A, Rotavirus B, and Rotavirus C. Other embodiments include methods and kits for detecting diarrhea causing pathogens.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
The present invention relates to a mixing apparatus for use in a fluid mixing equipment. In an embodiment, the mixing apparatus includes a hub having a top portion and a bottom portion and a central opening extending between the top and bottom portions along a centerline. A base flange extends outward from the bottom portion of the hub and one or more blades are coupled to the hub and extending to a shroud wall surrounding at least a portion of the top portion of the hub.
B01F 27/113 - Propeller-shaped stirrers for producing an axial flow, e.g. shaped like a ship or aircraft propeller
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
B01F 101/23 - Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
15.
COMPOSITIONS, KITS AND METHODS FOR DIRECT AMPLIFICATION FROM CRUDE BIOLOGICAL SAMPLES
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the detection of target nucleic acids, including those from microbes and/or from infectious agents such as SARS-CoV-2 and other viruses. Embodiments described herein are designed to enable processing and analysis of the sample to detect target nucleic acids within the sample without requiring extraction and/or isolation of nucleic acid from the sample prior to subsequent processing steps. Samples analyzed can thus be “crude” biological samples that do not require pre-processing prior to placement in the workflow.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
16.
MEMBRANE-PENETRATING PEPTIDES TO ENHANCED TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME
The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
Disclosed herein are methods of genotyping a crude nucleic acid sample, the methods including: subjecting the crude nucleic acid sample to a polymerase chain reaction (PCR) mixture directly after obtaining the crude nucleic acid sample from a source; incubating the crude nucleic acid sample in the PCR mixture for a set period of time; performing rapid PCR on the incubated crude nucleic acid sample; and analyzing results from the rapid PCR to determine the nucleic acid sample's genotype. Methods of detecting a disease in a subject are also disclosed.
Systems and methods for Raman spectroscopy. In one example, a method for analysing a sample (404, 530) includes irradiating the sample (404, 530) with a second light and acquiring a fluorescence signal (504), and adjusting a first light (305) based on the fluorescence signal (504). The method includes irradiating the sample (404, 530) with the adjusted first light (305) and acquiring a Raman signal, and analysing a sample composition based on the Raman signal.
The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.
Disclosed herein are methods of genotyping a crude nucleic acid sample, the methods including: subjecting the crude nucleic acid sample to a polymerase chain reaction (PCR) mixture directly after obtaining the crude nucleic acid sample from a source; performing rapid PCR on the crude nucleic acid sample by subjecting the crude nucleic acid sample to a first plurality of amplification cycles and followed by at least one second amplification cycle; and analyzing results from the rapid PCR to determine the nucleic acid sample's genotype. Each cycle of the first plurality of amplification cycles includes performing denaturation followed by annealing and extension without collecting amplification data. The at least second amplification cycle includes performing denaturation followed by annealing and extension while simultaneously collecting amplification data. Methods of detecting a disease in a subject are also disclosed.
INVITROGEN BIOSERVICES INDIA PRIVATE LIMITED (India)
Inventor
Rystrom, Larry
Ippolito, Kim
Rognin, Nicolas
K Y, Pradeep
Katiki, Naga Harshini
Hamilton, Mary Kristina
Kearns, Austin
Abstract
Systems and methods for identifying clumps (or groupings) of cells in cell counting systems. One example method executed by an electronic processing device for an image processing system includes receiving an image, captured by an imaging device, of a sample having a plurality of cells. The image includes labeling data for each of the plurality of cells. The method includes applying filters, determined based on the labeling data, to the image, processing the image to identify a plurality of groupings of the plurality of cells, and fitting an ellipse around each of the groupings by determining ellipse data for each of the ellipses. Each respective grouping of the plurality of groupings includes at least two cells having a same viability whose cell membranes are touching at least one other cell in the respective grouping.
Thermo Scientific Portable Analytical Instruments Inc. (USA)
Inventor
Brand, Audrey
Abstract
Systems and methods for conducting spectroscopic imaging, such as Raman spectroscopy. One example provides an optical analysis system includes a light source generating an excitation light and an attachment. The attachment includes a reflective surface positioned to reflect light from a sample toward a probe of the optical analysis system. The attachment includes a holder supporting the reflective surface, wherein the sample is positioned between the probe and the reflective surface.
INVITROGEN BIOSERVICES INDIA PRIVATE LIMITED (India)
Inventor
Rystrom, Larry
Ippolito, Kim
Rognin, Nicolas
K Y, Pradeep
Katiki, Naga Harshini
Hamilton, Mary Kristina
Kearns, Austin
Abstract
Systems and methods for identifying clumps (or groupings) of cells in cell counting systems. One example method executed by an electronic processing device for an image processing system includes receiving an image, captured by an imaging device, of a sample having a plurality of cells. The image includes labeling data for each of the plurality of cells. The method includes applying filters, determined based on the labeling data, to the image, processing the image to identify a plurality of groupings of the plurality of cells, and fitting an ellipse around each of the groupings by determining ellipse data for each of the ellipses. Each respective grouping of the plurality of groupings includes at least two cells having a same viability whose cell membranes are touching at least one other cell in the respective grouping.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventor
Brand, Audrey
Abstract
Systems and methods for conducting spectroscopic imaging, such as Raman spectroscopy. One example provides an optical analysis system includes a light source generating an excitation light and an attachment. The attachment includes a reflective surface positioned to reflect light from a sample toward a probe of the optical analysis system. The attachment includes a holder supporting the reflective surface, wherein the sample is positioned between the probe and the reflective surface.
Described herein are various embodiments and related methods of a needle and tubing organizing support system that can include one or more of a tray assembly configured to protect and retain a portion of a needle assembly and a securing mechanism. The tray assembly can include a tray body and a capturing recess extending along the tray body for at least partially containing a needle of the needle assembly. The tray assembly can further include a retention element and/or a sheath shaped to extend along a length of the capturing recess and encompass at least a portion of the needle of the needle assembly. The securing mechanism can be coupled to the tray body and configured to releasably secure a tubing of the needle assembly to the tray body.
A61M 5/00 - Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular wayAccessories therefor, e.g. filling or cleaning devices, arm rests
A61M 5/14 - Infusion devices, e.g. infusing by gravityBlood infusionAccessories therefor
A61M 5/32 - NeedlesDetails of needles pertaining to their connection with syringe or hubAccessories for bringing the needle into, or holding the needle on, the bodyDevices for protection of needles
A61M 39/08 - TubesStorage means specially adapted therefor
B65D 85/24 - Containers, packaging elements or packages, specially adapted for particular articles or materials for incompressible or rigid rod-shaped or tubular articles for needles, nails or like elongate small articles
Disclosed are microfluidic devices. The microfluidic device comprises microfeatures disposed in the loading conduit thereof, configured to separate impurities from a fluid flowing from the loading conduit to the sample compartments. The loading conduit comprises a filtration section comprising the microfeatures, and a narrow section in fluidic communication with the filtration section. The filtration section has a larger cross-sectional area than the cross-sectional area of the narrow section, resulting in added filtration capacity for the loading conduit of the microfluidic device.
A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.
A system for charge detection mass spectrometry (CDMS) performs a process including subdividing an m/z range of interest into a plurality of m/z windows; determining an ion population control parameter for each m/z window; accumulating, in the ion store by one or more accumulation events, a population of ions derived from a sample; transferring the population of ions to a mass analyzer; and mass analyzing the population of ions to acquire a CDMS spectrum of the population of ions. Each accumulation event corresponds to a distinct m/z window of the plurality of m/z windows. The ion population control parameter for each m/z window regulates a quantity of ions accumulated in an ion store during an accumulation event. During each accumulation event, ions within the m/z window corresponding to the accumulation event are accumulated in the ion store based on the ion population control parameter for the corresponding m/z window.
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
Inventor
Dugas, Michael
Abstract
According to one aspect, a system for identifying an element is described that includes an X-ray source configured to direct x-ray energy to a sample; a detector configured to detect fluorescent emissions from the sample; and a processor configured to: produce an X-ray fluorescent spectrum from the detected fluorescent emissions, where the X-ray fluorescent spectrum is representative of an elemental composition of the sample; calculate a first concentration value for each element in the elemental composition using a first method; select an element from the elemental composition using the first concentration value; and recalculate the concentration value of the selected element using a second method.
G01N 23/223 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence
33.
Apparatuses, Systems And Methods For Imaging Flow Cytometry
The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials
G01N 15/02 - Investigating particle size or size distribution
G01N 15/0227 - Investigating particle size or size distribution by optical means using imagingInvestigating particle size or size distribution by optical means using holography
Described herein are various embodiments and related methods of a needle and tubing organizing support system that can include one or more of a tray assembly configured to protect and retain a portion of a needle assembly and a securing mechanism. The tray assembly can include a tray body and a capturing recess extending along the tray body for at least partially containing a needle of the needle assembly. The tray assembly can further include a retention element and/or a sheath shaped to extend along a length of the capturing recess and encompass at least a portion of the needle of the needle assembly. The securing mechanism can be coupled to the tray body and configured to releasably secure a tubing of the needle assembly to the tray body.
According to one aspect, a system for identifying an element is described that includes an X-ray source configured to direct x-ray energy to a sample; a detector configured to detect fluorescent emissions from the sample; and a processor configured to: produce an X-ray fluorescent spectrum from the detected fluorescent emissions, where the X-ray fluorescent spectrum is representative of an elemental composition of the sample; calculate a first concentration value for each element in the elemental composition using a first method; select an element from the elemental composition using the first concentration value; and recalculate the concentration value of the selected element using a second method.
G01N 23/223 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence
36.
MULTIPLEX PANEL FOR DETECTING GASTROINTESTINAL BACTERIAL NUCLEIC ACIDS
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Described herein are various embodiments and related methods of a needle and tubing organizing support system that can include one or more of a tray assembly configured to protect and retain a portion of a needle assembly and a securing mechanism. The tray assembly can include a tray body and a capturing recess extending along the tray body for at least partially containing a needle of the needle assembly. The tray assembly can further include a retention element and/or a sheath shaped to extend along a length of the capturing recess and encompass at least a portion of the needle of the needle assembly. The securing mechanism can be coupled to the tray body and configured to releasably secure a tubing of the needle assembly to the tray body.
A61M 5/32 - NeedlesDetails of needles pertaining to their connection with syringe or hubAccessories for bringing the needle into, or holding the needle on, the bodyDevices for protection of needles
Methods for determining genotypes of structural variants in a sample genome, may include: amplifying nucleic acid sequences at targeted locations in the sample genome by a panel targeting a plurality of structural variant marker to generate sequence reads; mapping the sequence reads to a modified reference genome to produce aligned sequence reads, wherein the modified reference genome includes a wild-type target region and a structural variant target region; for each structural variant marker, determining a read count for a wild-type allele and a read count for a structural variant allele; determining a probability for each possible genotype, wherein the possible genotypes include a homozygous wild-type genotype, a heterozygous genotype and a homozygous structural variant genotype; and selecting the genotype with a maximum probability value to provide an estimated genotype corresponding to the structural variant marker of the sample.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
A method for determining a genotype of a sample of a polyploid organism, may include: amplifying nucleic acid sequences at targeted locations in a sample genome by a panel targeting a plurality of SNP markers to generate sequence reads; mapping the sequence reads to a reference genome for the polyploid organism; detecting variants in the aligned sequence reads to produce detected variants, wherein the detected variants include detected SNP's corresponding to the SNP markers; determining a probability for each alternate allele dosage of a plurality of possible allele dosages for a corresponding detected SNP, wherein the number of possible allele dosages is equal to a ploidy of the SNP marker plus one; and selecting the alternate allele dosage with a maximum probability value to provide an estimated allele dosage corresponding to the SNP marker, wherein the estimated allele dosage is indicative of the genotype for the SNP marker.
This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.
An x-ray system for measuring a thickness of a material includes a source configured to emit a fan beam of x-ray energy along a beam path. A filter is disposed in the beam path. The filter includes a first thickness positioned in a substantially peripheral region of the fan beam and a second thickness positioned in a substantially central region of the fan beam. The first thickness is different than the second thickness. A detector array includes a plurality of detectors. The detector array is configured to detect the x-ray energy transmitted through the filter. The x-ray energy strikes each detector at an angle associated with the region of the fan beam.
G01B 15/02 - Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons for measuring thickness
Systems and methods under the present disclosure include sample collection kits, such as for saliva or other fluids. In certain embodiments a detachable funnel can collect saliva from a user. A debris filter can be coupled to the funnel that extends into the test tube. The funnel can comprise or be coupled to a reagent cartridge that, when manipulated by a user, can break open and release reagent into the test tube for use in testing the saliva. Certain embodiments can comprise a casing that can house a test tube and tube cap, with a funnel configured to cover the entire top edge of the casing so as to protect the test tube and tube cap until used by a user.
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
The present disclosure discusses N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure discusses methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for multiplex biological assays.
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
An x-ray system for measuring a thickness of a material includes a source configured to emit a fan beam of x-ray energy along a beam path. A filter is disposed in the beam path. The filter includes a first thickness positioned in a substantially peripheral region of the fan beam and a second thickness positioned in a substantially central region of the fan beam. The first thickness is different than the second thickness. A detector array includes a plurality of detectors. The detector array is configured to detect the x-ray energy transmitted through the filter. The x-ray energy strikes each detector at an angle associated with the region of the fan beam.
G01B 15/02 - Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons for measuring thickness
Systems, methods, software and computer-usable media for annotating biomolecule-related data are disclosed. In certain exemplified embodiments, the biomolecules can be nucleic acids and the data can be sequence-related data. In various embodiments, systems can include one or more public or private biological attributes (e.g., annotation information databases, data storage devices and systems, etc.) sources, one or more genomic features data sources (e.g., genomic variant tools, genomic variant databases, genomic variant data storage devices and systems, etc.), a computing device (e.g., workstation, server, personal computer, mobile device, etc.) hosting an annotations module and/or a reporting module, and a client terminal.
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
A barcode for use in nucleic acid sequencing includes a combinatorial barcode sequence including at least two iterations of a sequence motif, the sequence motif comprising a first nucleotide base from a first group of nucleotide bases, followed by a second nucleotide base from a second group of nucleotide bases, followed by a third nucleotide base from a third group of nucleotide bases. The first group, the second group, and the third group differ from each other; two groups of the first, second, and third groups contain at least two different nucleotide bases; and at least one of the first, second, and third groups comprises at least three different nucleotide bases; and the combinatorial barcode sequence is one of at least eight potential combinatorial barcode sequences exhibiting the plurality of attributes, and the potential combinatorial barcode sequences are synchronized in flow space based on the predetermined order of nucleotide flows.
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 70/00 - Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or barcodes
50.
BARCODE SEQUENCES, AND RELATED SYSTEMS AND METHODS
Methods, system, and kits are provided for sample identification, and, more specifically, for designing, and/or making, and/or using sample discriminating codes or barcodes for identifying sample nucleic acids or other biomolecules or polymers. For example, a plurality of flowspace codewords may be generated, the codewords comprising a string of characters. A location for at least one padding character within the flowspace codewords may be determined. The padding character may be inserted into the flowspace codewords at the determined location. After the inserting, a plurality of the flowspace codewords may be selected based on satisfying a predetermined minimum distance criteria, wherein the selected codewords correspond to valid base space sequences according to a predetermined flow order. And the barcode sequences corresponding to the selected codewords may be manufactured.
A method of performing a digital polymerase chain reaction (dPCR) includes performing an amplification reaction on a reaction mixture including a crude lysate sample to generate amplicons of a target nucleic acid of a pathogen, wherein the crude lysate sample includes biological tissue. Fluorescence is detected from a probe hybridized to the amplicons. A method of detecting pathogen in a subject includes performing the above amplification reaction. The crude lysate sample includes biological tissue from the subject. The fluorescence detected from the probe hybridized to the amplicons is used to determine if the pathogen is present in the subject. Compositions, PCR systems, and kits are also provided.
A method for quality control is executable by an electronic processing device. The method includes receiving a spectrum collected from a sample, inputting the spectrum to an autoencoder trained with a plurality of training spectra belonging to a class, and indicating whether the spectrum is a member of the class based on an output from the trained autoencoder.
A method of performing automatic ion control for mass spectrometry includes acquiring, by charge detection mass spectrometry, a mass spectrum comprising a plurality of peaks representing intensity as a function of mass-to-charge ratio (m/z) of a population of ions analyzed by a mass analyzer during an acquisition event. Based on the mass spectrum, a measured signal density of a selected m/z range of the mass spectrum is determined. An ion population control parameter for a subsequent acquisition event is set based on the measured signal density and a target signal density. The ion population control parameter regulates a population of ions analyzed by the mass analyzer during the subsequent acquisition event.
Systems, methods, and computer readable media with instructions for automated thresholding of dPCR assay data include generating a histogram from emission data collected from reaction sites of a dPCR assay, the histogram comprising a plurality of bins representing a number of reaction sites at different levels of emission signal; based on a cluster of high concentration bins (representing data that is positive or negative for amplification product based on emission signal level), identifying bins having low concentration based on a number of reaction sites per bin being less than or equal to a predetermined amount, and then setting a threshold based on lowest or highest emission signal levels in the low concentration bins.
A method for conjugating oligonucleotides to bead supports includes adding carbodiimide to an aqueous suspension including bread supports having carboxy functional groups; adding hydroxysuccinimide to the aqueous suspension; and adding amine modified oligonucleotide to the aqueous suspension.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
A method for conjugating oligonucleotides to bead supports includes adding carbodiimide to an aqueous suspension including bread supports having carboxy functional groups; adding hydroxysuccinimide to the aqueous suspension; and adding amine modified oligonucleotide to the aqueous suspension.
New lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells and tissue. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes and complexes for in vivo delivery of bioactive agents.
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
C07C 225/20 - Compounds containing amino groups and doubly-bound oxygen atoms bound to the same carbon skeleton, at least one of the doubly-bound oxygen atoms not being part of a —CHO group, e.g. amino ketones having amino groups bound to carbon atoms of rings other than six-membered aromatic rings of the carbon skeleton
C07D 257/02 - Heterocyclic compounds containing rings having four nitrogen atoms as the only ring hetero atoms not condensed with other rings
C07D 295/135 - Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly or doubly bound nitrogen atoms with the ring nitrogen atoms and the substituent nitrogen atoms separated by carbocyclic rings or by carbon chains interrupted by carbocyclic rings
61.
System and Method for Aqueous Conjugation of Substrates Using Diels-Alder Chemistry
A method for conjugating oligonucleotides to bead supports includes adding 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium to an aqueous suspension including bread supports having carboxy functional groups; adding amino-maleimide to the aqueous suspension; and adding diene modified oligonucleotide to the aqueous suspension.
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
A method for enriching clonal populations includes, for a population of target nucleic acids, includes exposing the population of target nucleic acids to a plurality of supports; amplifying the bound target nucleic acids in the presence of a primer to form supports including a plurality of copies of the target nucleic acids of the population of target nucleic acids; applying to the supports a capture primer; applying a magnetic bead functionalize with a moiety to bind to the binder moiety; and separating the first set of supports from the second and third set of supports.
A vacuum system for a mass spectrometer includes a first vacuum region, a second vacuum region, a vacuum interlock fluidly connected to the vacuum chamber by a first valve, a first pump fluidly connected to the first and second vacuum regions, a second pump fluidly connected to the second vacuum region, and a second valve. The second valve includes a housing, a piston movable within the housing between an evacuation position and an operation position, a first channel connected to a third valve, a second channel connected to the vacuum interlock, a third channel connected to the first pump, and a fourth channel connected to the second vacuum region. In response to the third valve adjusting to a first position, the piston moves to the evacuation position. In response to the third valve adjusting to the second position, the piston moved to the opened position.
H01J 49/24 - Vacuum systems, e.g. maintaining desired pressures
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
H01J 49/26 - Mass spectrometers or separator tubes
64.
METHOD FOR AQUEOUS CONJUGATION OF SUBSTRATES USING DIELS-ALDER CHEMISTRY
A method for conjugating oligonucleotides to bead supports includes adding 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium to an aqueous suspension including bread supports having carboxy functional groups; adding amino-maleimide to the aqueous suspension; and adding diene modified oligonucleotide to the aqueous suspension.
A method for enriching clonal populations includes, for a population of target nucleic acids, includes exposing the population of target nucleic acids to a plurality of supports; amplifying the bound target nucleic acids in the presence of a primer to form supports including a plurality of copies of the target nucleic acids of the population of target nucleic acids; applying to the supports a capture primer; applying a magnetic bead functionalize with a moiety to bind to the binder moiety; and separating the first set of supports from the second and third set of supports.
A chromatographic media for separating biopolymers, the chromatographic media having hydrophobic and ionic retention modes, the chromatographic media comprising porous substrate particles including a hydrophobic polymer resin and ion exchange functional groups copolymerized with the hydrophobic polymer resin or grafted to the surface of the porous substrate, wherein the ion exchange functional groups are not greater than about 5 mol% of the porous substrate particles.
B01D 15/32 - Bonded phase chromatography, e.g. with normal bonded phase, reversed phase or hydrophobic interaction
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
B01J 41/07 - Processes using organic exchangers in the weakly basic form
B01J 41/14 - Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
B01J 41/20 - Anion exchangers for chromatographic processes
B01J 43/00 - Amphoteric ion-exchange, i.e. using ion-exchangers having cationic and anionic groupsUse of material as amphoteric ion-exchangersTreatment of material for improving their amphoteric ion-exchange properties
B01J 47/014 - Ion-exchange processes in generalApparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
Methods and apparatuses of determining sorting efficiency for sorted particles. One method includes acquiring event statistics based on one or more signals generated from interrogating the particles in a fluid stream. The method also includes determining a degree of deviation of the event statistics from reference statistics and taking an automated responsive action in response to the degree of deviation exceeding a threshold level.
Embodiments of the invention are disclosed that provide improved computer systems, computerized methods, and computer program products for generating and evaluating automated predictions regarding whether a particular amplification curve from a qPCR assay indicates presence of a target molecule in a sample. In some embodiments, predictions are generated using deep learning networks. In some embodiments, curve-quality predictions are generated and used to assess whether an amplification prediction can be reliably made from a particular amplification curve or whether the curve reflects an anomaly in the qPCR assay. In various embodiments, prediction confidence data is also generated and used, along with prediction data, in an electronic user interface to improve qPCR measurement.
Methods and apparatuses of determining sorting efficiency for sorted particles. One method includes acquiring event statistics based on one or more signals generated from interrogating the particles in a fluid stream. The method also includes determining a degree of deviation of the event statistics from reference statistics and taking an automated responsive action in response to the degree of deviation exceeding a threshold level.
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
G01N 15/1404 - Handling flow, e.g. hydrodynamic focusing
A61M 1/36 - Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation
G01N 15/12 - Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
G01N 15/1409 - Handling samples, e.g. injecting samples
G01N 21/17 - Systems in which incident light is modified in accordance with the properties of the material investigated
G01N 29/22 - Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic wavesVisualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object Details
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
74.
METHODS AND SYSTEMS FOR ALIGNING AN OPTICAL INSTRUMENT
An optical steering mechanism and methods for using the same. One optical steering mechanism includes a first lens defining a first focal length having a first magnitude and a first polarity and a second lens defining a second focal length having a second magnitude and a second polarity. The first and second magnitudes are substantially equal and the first and second polarities are opposite, and wherein the second lens is positioned to directly receive an optical beam passing through the first lens. The optical beam steering mechanism also includes at least one rotary motor coupled to one of the first lens and the second lens and configured to swing the lens coupled thereto in an arcuate path. An optical beam path of the optical beam passed through the second lens is adjustable by operating the rotary motor.
G02F 1/29 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulatingNon-linear optics for the control of the position or the direction of light beams, i.e. deflection
A vacuum system for a mass spectrometer includes a first vacuum region, a second vacuum region, a vacuum interlock connected to the first vacuum region by a first valve and the second vacuum region by a second valve, a first pump connected to the first and second vacuum regions, a second pump connected to the second vacuum region, a pressure sensor configured to determine the pressure within the second vacuum region, and a controller configured to adjust a position of the second valve in response to the pressure within the second vacuum region. The vacuum interlock is configured to receive a sample. The first pump is configured to decrease a pressure within the first vacuum region and exhaust air to the second vacuum region. The second pump is configured to decrease a pressure within the vacuum interlock. The controller is configured to prevent pressure fluctuations in the second vacuum region.
Methods and systems that use cloud-based resources and assay definition files for a local server system to control a sequencing device and process sequencing data resulting from a sequencing run for an assay are described. A method may include receiving, at a local server system, an assay definition file from a server of a cloud computing and storage system. The assay definition file may include code modules for configuring an assay. The code modules may be stored in a memory of the local server system. The server system may receive sequencing data from a sequencing device. The sequencing device may produce the sequencing data during a sequencing run performed for the assay. The server system may apply an analysis pipeline for the assay to the sequencing data. The analysis pipeline includes analysis steps executed in accordance with the code modules from the assay definition file to produce assay analysis results.
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
G16H 40/40 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the management of medical equipment or devices, e.g. scheduling maintenance or upgrades
G16H 40/67 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
78.
COMPOSITION AND METHOD FOR DETECTION OF BIOMOLECULES VIA AROMATIC LABELING USING HETEROCYCLIC COMPOUNDS
Disclosed herein is a composition comprising a medium and a heterocyclic compound, for detecting a biomolecule in a sample. More specifically, the composition disclosed herein can be used to detect one or more biomolecules comprising one or more aromatic amino acid residues by producing an amino acid conjugate upon associating with the aromatic amino acid residue of the biomolecule. Also disclosed herein are methods of making and using the composition disclosed herein.
Disclosed herein is a composition comprising a medium and a heterocyclic compound, for detecting a biomolecule in a sample. More specifically, the composition disclosed herein can be used to detect one or more biomolecules comprising one or more aromatic amino acid residues by producing an amino acid conjugate upon associating with the aromatic amino acid residue of the biomolecule. Also disclosed herein are methods of making and using the composition disclosed herein.
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.
A method of identifying a copy number variations reads includes mapping reads to a reference genome, computing coverage for a plurality of tiles, and normalizing the coverage for a tile based on a coverage mode across the plurality of tiles. The method further includes determining a score for the plurality of tiles being in a plurality of ploidy states, determining a maximum score path across the tiles and through the ploidy states, and providing a copy number determination based on the maximum score path.
A method for nucleic acid sequencing may include disposing a plurality of template nucleic acid molecules in a plurality of defined spaces disposed on a sensor array, at least some of the plurality of template nucleic acid molecules having a sequencing primer and a polymerase operably bound therewith; advancing one or more nucleotide species over the plurality of template nucleic acid molecules with the sequencing primer and the polymerase operably bound therewith; measuring a signal generated by nucleotide incorporations resulting from advancing the one or more nucleotide species; and exposing the plurality of template nucleic acid molecules to a cleaving reagent subsequent to the advancing and measuring. The cleaving reagent can remove labeling reagents attached to the one or more nucleotide species. The advancing and measuring steps can be performed for different orders of the one or more nucleotide species prior to a subsequent exposing of the plurality of template nucleic acid molecules to the cleaving reagent.
Methods, systems, and apparatuses for navigating data visualizations within a user interface. One method includes providing particle data, which may be captured by an imaging device, to the user interface and receiving, from the user interface, a plurality of coordinates for a gate related to the particle data. The method also includes receiving, from the user interface, a configuration for a current view of the particle data, and, in response to determining the gate is off-plot for the current view based on the plurality of coordinates for the gate and the configuration for the current view, adding a selectable indicator to the current view of the particle data representing the gate. The method further includes, in response to receiving a selection of the indicator, modifying the current view of the particle data to display the gate.
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.
Methods, systems, and apparatuses for navigating data visualizations within a user interface. One method includes providing particle data, which may be captured by an imagin device, to the user interface and receiving, from the user interface, a plurality of coordinates for a gate related to the particle data. The method also includes receiving, from the user interface, a configuration for a current view of the particle data, and, in response to determining the gate is off-plot for the current view based on the plurality of coordinates for the gate and the configuration for the current view, adding a selectable indicator to the current view of the particle data representing the gate. The method further includes, in response to receiving a selection of the indicator, modifying the current view of the particle data to display the gate.
A system for gas chromatography includes an inlet configured to receive a sample by injection, a column having a stationary phase, a flow control system, and an injection monitoring system. The flow control system is configured to regulate, based on a flow control parameter, flow of a mobile phase through the inlet and the column. The injection monitoring system is configured to obtain flow control data representative of a measure of the flow control parameter over time during a time period encompassing an injection of the sample into the inlet; determine, based on the flow control data, that the injection was unsuccessful; and perform, based on the determination that the injection was unsuccessful, a mitigation operation to mitigate the unsuccessful injection of the sample.
G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
B01D 53/02 - Separation of gases or vapoursRecovering vapours of volatile solvents from gasesChemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
B01D 53/30 - Controlling by gas-analysis apparatus
An adjustment device (100) for a microplate reader (1000), includes: an actuation mechanism (10) connected to a housing (1000A) of the microplate reader (1000); an adjustable aperture (20) including an aperture opening (21), wherein the adjustable aperture (20) is driven to move in an axial direction by an actuation mechanism (10); and a diaphragm opening adjusting member (30) configured to be movable relative to the center of the aperture opening (21) so as to define an adjustable diaphragm opening (22) in the adjustable aperture (20), where the diaphragm opening adjusting member (30) is movable between a first position and a second position as the adjustable aperture (20) moves towards or away from a microplate (201) in the axial direction, and the size of the adjustable diaphragm opening (22) in the first position is greater than the size of the adjustable diaphragm opening (22) in the second position. The adjustment device (100) can change the size of the adjustable diaphragm opening (22) while the axial position of the adjustable aperture (20) changes. In addition, a microplate reader (1000) includes an adjustment device (100).
Disclosed herein are machine learning-based particle classification systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a particle classification system may include: an electronic processing device configured to: receive, from an imaging flow cytometer instrument, a test set comprising unlabeled data to classify; pool the test set and a training set into a concatenated dataset comprising a plurality of parameters, wherein the training set comprises labeled data; normalize the concatenated dataset by bringing the variance to one for each of the plurality of parameters; non-linearly reduce a dimensionality of the normalized concatenated dataset to a reduced dimension space; compute classification parameters by classifying the unlabeled data from the reduced dimension space using the labeled data from the reduced dimension space; and provide the classification parameters for further processing.
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
G06F 18/2413 - Classification techniques relating to the classification model, e.g. parametric or non-parametric approaches based on distances to training or reference patterns
G06V 10/762 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using clustering, e.g. of similar faces in social networks
G06V 10/774 - Generating sets of training patternsBootstrap methods, e.g. bagging or boosting
90.
METHODS AND SYSTEMS FOR VISUALIZING AND EVALUATING DATA
A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
91.
SYSTEMS AND METHODS FOR PARTICLE CLASSIFICATION USING MACHINE LEARNING
Disclosed herein are machine learning-based particle classification systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a particle classification system may include: an electronic processing device configured to: receive, from an imaging flow cytometer instrument, a test set comprising unlabeled data to classify; pool the test set and a training set into a concatenated dataset comprising a plurality of parameters, wherein the training set comprises labeled data; normalize the concatenated dataset by bringing the variance to one for each of the plurality of parameters; non-linearly reduce a dimensionality of the normalized concatenated dataset to a reduced dimension space; compute classification parameters by classifying the unlabeled data from the reduced dimension space using the labeled data from the reduced dimension space; and provide the classification parameters for further processing.
Business management of clinical trials for others;
recruitment of clinical drug trial participants; marketing
of healthcare and pharmaceutical products of others;
compiling information into computer databases regarding
health conditions, clinical trial participation interest,
and clinical trial candidacy for business purposes;
collection of market research information, namely,
collecting and compiling clinical trial statistics and
information for business purposes.
93.
SAMPLE PREPARATION, PROCESSING AND ANALYSIS SYSTEMS
A cartridge module comprising a cartridge receptacle configured to receive and hold a cartridge that comprises a plurality of chambers and fluidic channels and a plurality of valves for regulating fluid flow to and from the chambers via the fluidic channels; a manifold configured to engage a first side of the cartridge and thereby bring the chambers in fluidic communication with a source of positive pressure or negative pressure; and a plurality of springs configured to actuate a thermocycling device.
A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.
An instrument includes a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to close off a fluidic channel in the body. A cartridge interface is configured to engage the cartridge.
C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
C07K 5/02 - Peptides having up to four amino acids in a fully defined sequenceDerivatives thereof containing at least one abnormal peptide link
C07K 5/062 - Dipeptides the side chain of the first amino acid being acyclic, e.g. Gly, Ala
C07K 5/103 - Tetrapeptides the side chain of the first amino acid being acyclic, e.g. Gly, Ala
97.
NUCLEOTIDE TRANSIENT BINDING FOR SEQUENCING METHODS
Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
A high data rate integrated circuit, such as an integrated circuit including a large sensor array, may be implemented using clock multipliers in individual power domains, coupled to sets of transmitters, including a transmitter pair configuration. Reference clock distribution circuitry on the integrated circuit distributes a relatively low speed reference clock. In a transmitter pair configuration, each pair comprises a first transmitter and a second transmitter in a transmitter power domain. Also, each pair of transmitters includes a clock multiplier connected to the reference clock distribution circuitry, and disposed between the first and second transmitters, which produces a local transmit clock.
Disclosed are compositions, kits, and methods for use in applications involving electrophoretic separation of nucleic acids, including capillary electrophoresis applications in which nucleic acid samples are labelled with dyes, size-separated, and subjected to short tandem repeat (STR) analysis. A sample containing DNA is mixed with a composition that includes at least one set of primers that target an STR region of the sample nucleic acid, a set of short quantification primers (QS primers) that target a first multi-copy sequence (QS sequence) of the sample nucleic acid, and a set of long quantification primers (QL primers) that target a second multi-copy sequence (QL sequence) of the sample nucleic acid, wherein the QS sequence is shorter than the QL sequence.
Life Technologies Holdings PTE Limited (Singapore)
Inventor
Murakami, Marie
Bulloch, Kyle
Olson, Neil
Winnick, Ross
Teo, Wei Fuh
Thacker, Michael
Abstract
The present disclosure provides systems for gel electrophoresis and electrotransfer comprising one or more chambers that can removably and interchangeably receive either an electrophoresis cassette, or an electrotransfer cassette, and provides an electrical interface for both electrophoresis and electrotransfer of biomolecules. The present disclosure also provides electrophoresis devices including clamps and electrotransfer cassettes and related devices. Methods for electrophoresis and electrotransfer using the systems and devices of the disclosure are also provided.
