Abrégé

Disclosed herein are PET hydrolase enzymes, and their nucleic acid and amino acid sequences. A number of candidates have been identified with detectable, quantifiable activity on PET and these enzymes possess desirable traits that are leveraged in the design and engineering of enzyme formulations targeted to degrade specific polymers. These enzymes have measurable PET degrading activity and, in an embodiment, may be active polyester polyurethanes.

Classes IPC  ?

• B09B 3/60 - Traitement biochimique, p. ex. en utilisant des enzymes
• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 9/18 - Hydrolases agissant sur les esters d'acides carboxyliques
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• B09B 101/75 - Déchets plastiques

Abrégé

The present disclosure relates to a non-naturally occurring enzyme that includes a first polypeptide that catalyzes the hydrolysis of a polyester to produce mono-(2-hydroxyethyl) terephthalate (MHET), a second polypeptide that catalyzes the cleavage of MHET to produce at least one of terephthalic acid or ethylene glycol, and a third polypeptide that links the first polypeptide with the second polypeptide.

Classes IPC  ?

• C12N 9/18 - Hydrolases agissant sur les esters d'acides carboxyliques
• C08J 11/10 - Récupération ou traitement des résidus des polymères par coupure des chaînes moléculaires des polymères ou rupture des liaisons de réticulation par voie chimique, p. ex. dévulcanisation
• B09B 3/60 - Traitement biochimique, p. ex. en utilisant des enzymes

Abrégé

Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.

Classes IPC  ?

• C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
• C12N 9/42 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons bêta-glucosidiques-1, 4, p. ex. cellulase

Abrégé

The invention relates to a method of immobilising at least one RNA molecule onto a surface of a support comprising: i) providing a first support having a surface on which at least one DNA molecule is immobilised, wherein the DNA molecule encodes an RNA molecule and the encoded RNA molecule comprises a binding molecule; ii) providing a second support having a surface on which at least one binding partner for interacting with the binding molecule is immobilised; iii) arranging the first and second supports such that the surfaces displaying the immobilised molecules are in close proximity and substantially face each other, and contacting the DNA molecule immobilised on the surface of the first support with transcription reagents; and iv) carrying out a transcription reaction to generate the encoded RNA molecule, wherein the RNA molecule is directly immobilised onto the surface of the second support via an interaction between the binding molecule of the RNA molecule and the binding partner on the surface of the second support.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens