Abstract

Disclosed herein are PET hydrolase enzymes, and their nucleic acid and amino acid sequences. A number of candidates have been identified with detectable, quantifiable activity on PET and these enzymes possess desirable traits that are leveraged in the design and engineering of enzyme formulations targeted to degrade specific polymers. These enzymes have measurable PET degrading activity and, in an embodiment, may be active polyester polyurethanes.

IPC Classes  ?

• B09B 3/60 - Biochemical treatment, e.g. by using enzymes
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/18 - Carboxylic ester hydrolases
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• B09B 101/75 - Plastic waste

Abstract

The present disclosure relates to a non-naturally occurring enzyme that includes a first polypeptide that catalyzes the hydrolysis of a polyester to produce mono-(2-hydroxyethyl) terephthalate (MHET), a second polypeptide that catalyzes the cleavage of MHET to produce at least one of terephthalic acid or ethylene glycol, and a third polypeptide that links the first polypeptide with the second polypeptide.

IPC Classes  ?

• C12N 9/18 - Carboxylic ester hydrolases
• C08J 11/10 - Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
• B09B 3/60 - Biochemical treatment, e.g. by using enzymes

Abstract

Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.

IPC Classes  ?

• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase

Abstract

The invention relates to a method of immobilising at least one RNA molecule onto a surface of a support comprising: i) providing a first support having a surface on which at least one DNA molecule is immobilised, wherein the DNA molecule encodes an RNA molecule and the encoded RNA molecule comprises a binding molecule; ii) providing a second support having a surface on which at least one binding partner for interacting with the binding molecule is immobilised; iii) arranging the first and second supports such that the surfaces displaying the immobilised molecules are in close proximity and substantially face each other, and contacting the DNA molecule immobilised on the surface of the first support with transcription reagents; and iv) carrying out a transcription reaction to generate the encoded RNA molecule, wherein the RNA molecule is directly immobilised onto the surface of the second support via an interaction between the binding molecule of the RNA molecule and the binding partner on the surface of the second support.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides