The present invention relates to a porous polymer membrane having the combination of a specific average pore diameter, a specific thickness, and a specific convective porosity. Moreover, the present invention relates to a method of manufacturing such porous polymer membrane.
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
In a method of referencing an analyte measurement in a purification system during a process step in a biopharmaceutical process, the following sub-steps are performed during the same process step: guiding a medium through a purification unit where the analyte is either removed from or added to the medium; measuring at least one parameter related to the presence and/or quantity of the analyte with a first measurement system at a first measurement location upstream of the purification unit; measuring the at least one parameter with a second measurement system at a second measurement location downstream of the purification unit; and referencing the upstream measurement to the downstream measurement, or referencing the downstream measurement to the upstream measurement.
The present invention relates to a method for releasing a viral vector from a cell culture, wherein a cell of the cell culture is not required to be separated from the surrounding liquid prior to contacting the cell culture with the lysis reagent. The present invention further relates to a method for producing a viral vector inter alia comprising releasing the viral vector from a cell culture according to disclosed method for releasing a viral vector from a cell culture. The present invention further relates to a cell lysis reagent for releasing a viral vector from a cell culture. The present invention further relates to a kit for releasing a viral vector from a cell culture, wherein the kit comprises a container that contains the disclosed cell lysis reagent.
The present invention provides methods for releasing a viral vector from a cell culture. Specifically, the cell culture is contacted with a lysis reagent to generate a lysis composition and incubated, whereupon the lysis composition is contacted with an acidifying reagent to lower the pH. The method according to the present invention results in high viral vector release and reduction in co-released host cell related impurities. The present invention further provides a method for producing a viral vector. The present invention further provides a kit for releasing a viral vector from a cell culture. The present invention further provides a kit for producing a viral vector.
The invention relates to a method for controlling a bioprocess (1), wherein in a model control routine (5) a bioprocess control unit (4) controls at least one process parameter (6) of the bioprocess (1) according to a model-based control loop (7). It is proposed that during the model control routine (5) the bioprocess control unit (4) repeatedly performs a first evaluation (13) of the model-based control loop (7), that if a first decision criterion is fulfilled, the bioprocess control unit (4) switches from the model control routine (5) to a sample control routine (15), that in the sample control routine (15) the bioprocess control unit (4) controls the bioprocess (1) according to a sample-based control loop (23), that at least one sample input parameter (24) of the sample-based control loop (23) is derived from a measurement value.
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G05B 13/04 - Adaptive control systems, i.e. systems automatically adjusting themselves to have a performance which is optimum according to some preassigned criterion electric involving the use of models or simulators
G05B 17/02 - Systems involving the use of models or simulators of said systems electric
6.
METHOD OF CONTROLLING A BIOPROCESS ARRANGEMENT TO PROVIDE MEASUREMENT DATA TO A BIOPROCESS CONTROL SYSTEM
The invention relates to a method of controlling a bioprocess arrangement (1) to provide measurement data to a bioprocess control system (3), wherein the bioprocess arrangement (1) comprises a biocontainer arrangement (2), a sampling arrangement (4), a routing arrangement (6) and an analysis arrangement (7). It is proposed that the bioprocess control system (3) sends a measurement request to the control layer (9), that the measurement request contains information about a requested measurement, that the measurement request is independent of routing and analysis behind the sampling arrangement (4), that the sampling arrangement (4) draws the sample and provides the sample to the routing arrangement (6), that the control layer (9) coordinates the provision of the sample to the analysis arrangement (7) via the routing arrangement (6), that the analysis arrangement (7) sends measurement data to the control layer (9), and, that the control layer (9) sends the measurement data to the bioprocess control system (3).
DEVICE FOR ARRANGING ON A FLUID-CONDUCTING LINE AND FOR ATTACHING A FLOWMETER, AND METHOD FOR DETECTING A MEASUREMENT VARIABLE OF THE FLUID BEING CONDUCTED BY A LINE
A device for arranging on a fluid-conducting line and for attaching a flowmeter, in particular an ultrasonic flowmeter, for detecting a measurement variable of the fluid conducted by the line has a first and a second connection, and a measurement region arranged between the first connection and the second connection. The first connection, the measurement region, and the second connection define a flow path for the fluid through the device, and a flow-influencing element arranged in and/or on the flow path is configured to cause fluid flowing into the device via the first connection with a substantially laminar flow to have a substantially turbulent flow in the measurement region.
G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters
G01F 15/18 - Supports or connecting means for meters
8.
APPLICATION OF PERMITTIVITY MEASUREMENT PROBES IN AN SUSPENSION CULTURE AGGREGATE COMPRISING CELL AGGREGATES
The present disclosure relates to a method of measuring cell density in a cell suspension comprising cell aggregates, the method comprising (i) Measuring the permittivity of the cell suspension; (ii) Comparing the measured permittivity with a predetermined value that is indicative of the cell density, thereby determining the cell density. Further described is a of a permittivity probe for determining the cell density of a suspension cell culture comprising cell aggregates.
G01N 33/487 - Physical analysis of biological material of liquid biological material
G01N 27/02 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
9.
TEST PLAQUE FOR VALIDATION OF INJECTION MOULDING MATERIAL RHEOLOGICAL CHARACTERISATION
The invention relates to a test specimen (1) for rheological testing of plastic products comprising a plate body (10) from a plastic material, said plate body (10) having a first major surface (102), a second major surface opposite to the first major surface (102) and a side surface (106) connecting the first and the second major surface (104) and at least one spatial feature (12A, 12B, 14A, 14B) arranged in or on the plate body (10), each spatial feature (12A, 12B, 14A, 14B) indicative of a defect occurring in the plastic products or a geometric feature of the plastic products. The invention relates further to methods and respective systems using the test specimen, including methods and systems for testing for testing of a plastic product, for validating a rheological data set or a model of a process for manufacturing of plastic products, for determining at least one property of a plastic product to be manufactured and for manufacturing a plastic product.
The present invention concerns methods for processing and/or purification of a biological product involving multi-angle light scattering as analytical tool for process control. The methods of the invention apply multi-angle light scattering to detect the biological product, the presence of a bioburden or the functionality and/or integrity of a consumable employed in the method for processing and/or purification of the biological product. Further, the invention relates to systems for processing and/or purification of a biological product configured to perform the methods of the invention. Lastly, the invention concerns computer program products which can be used in the methods of the invention.
SYSTEM FOR DETECTING A MEASUREMENT VARIABLE OF A FLUID CONDUCTED IN A FLUID-CONDUCTING LINE, FLOW-THROUGH DEVICE FOR ARRANGING ON A FLUID-CONDUCTING LINE AND FOR ATTACHING A FLOWMETER, AND USE OF A FLOW-THROUGH DEVICE
A system for detecting a measurement variable of a fluid being conducted in a fluid-conducting line, having a flowmeter for detecting the measurement variable, a flow-through device for arranging on the fluid-conducting line and for attaching the flowmeter. The flowmeter has a first and second connection and a measurement region which is arranged between the first connection and the second connection and which can be coupled to the flowmeter. The first connection, the measurement region, and the second connection define a flow path for the fluid through the flow-through device.
G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters
G01F 15/18 - Supports or connecting means for meters
12.
BIOREACTOR FOR CELL CULTURE WITH REUSABLE REACTOR VESSEL AND OPTICAL SPECTROSCOPY INTERFACE
A bioreactor for cell cultivation including a reusable reactor vessel, preferably made of stainless steel, for receiving a fluid, and an optical spectroscopy interface including an adapter and a single-use optical spectroscopy insert. The reusable reactor vessel has a wall and a port formed in the wall. The adapter is configured to be fixed to the port in a predefined position at the port. The single-use optical spectroscopy insert is configured to be accommodated and fixed in the adapter, or the insert is an integral part of the adapter.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
13.
METHOD OF PERFORMING GAS/LIQUID-BASED INTEGRITY TEST
The present invention relates to a method of performing a gas/liquid-based integrity test, wherein air in a first membrane and a second membrane and present therebetween is replaced by a water-soluble gas before wetting the membranes. Further, the present invention relates to a method of producing an integrity-tested object having a first membrane, a second membrane and at least one boundary member.
MULTIPORT DEVICE FOR CONNECTING A LOOP TO ONE PORT OF A BIOREACTOR, AND PERFUSION OR CONCENTRATED FED-BATCH SETUP FOR PERFORMING AN UPSTREAM PROCESS OF CELL CULTURE
A multiport device for connecting a loop, preferably a tangential flow filtration loop or a sensor loop, to one port of a bioreactor, preferably a single-use bioreactor, is configured to be fixed to the port of the bioreactor. The multiport device includes a first flow path configured for withdrawing fluid from the bioreactor, and a second flow path configured for supplying fluid to the bioreactor. The first flow path has a first end adapted to be in fluid connection with the bioreactor, and a second end adapted to be connected to an inlet of the loop. The second flow path has an outer end adapted to be connected to an outlet of the loop, and a mouth adapted to be in fluid connection with the bioreactor. The mouth of the second flow path is distanced from the first end of the first flow path by at least 5 mm, preferably 10 mm.
Some aspects of the disclosure are related to systems and methods for controlled oxygen release from biomaterials in vessels and unit operations or components of cell culture, cell containment, and/or bioreactor. Vessels, unit operations, devices, and/or components of the invention may be used to perform all or part of a biological and/or chemical process involving biologicals (e.g., a plurality of cells) in the presence of oxygen-releasing agents. In some embodiments, a system comprises a vessel comprises an oxygen-releasing agent configured to generate in-situ and release oxygen in a sustained manner. The presence of an oxygen-releasing agent may advantageously allow for high cell density fermentation and cell cultivation in a vessel and provide an alternative for supplemental gassing means (e.g., sparger, etc.). Some embodiments of the disclosure are directed to employing the oxygen-releasing agents in microfluidic or millifluidic systems.
A separation system and methods for separating and purifying a target component include a separation system for separating and purifying a target component, a method for separating and purifying a target component, and the use of a single-pass crossflow diafiltration unit for integrally connecting a first and a second chromatography device.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
Containers including internal mixers and actuators assemblies and related methods are generally described. In some embodiments, a container may include a mixer and an actuator, at least one of which may be located inside of the container. The actuator may actuate the mixer to induce flow within the container and mix the contents of the container with a low volumetric footprint and high mixing efficiency. In some embodiments, the actuator may be configured to deform the mixer, which may include one or more features which may be deformed out of plane in a Kirigami or Origami fashion. The actuator and mixer may be arranged in series or in parallel. The actuator may be used without a mixer to induce flow within the container. The actuator may be driven pneumatically, hydraulically, electrically, and/or in any other suitable manner.
A bioreactor having a filter unit and a method for treating a cell broth. The filter unit has a supply channel (2), a first filter medium (4), a retentate channel (1), a second filter medium (5) and a permeate channel (3), arranged so that the first filter medium delimits the supply channel and the retentate channel from one another; and the second filter medium delimits the retentate channel and the permeate channel from one another. The supply channel is connected to an inlet for a supply medium; the retentate channel is connected to an inlet for a cell broth and to an outlet for the cell broth; the permeate channel is connected to an outlet for a permeate; and the interior of the bioreactor is connected to the inlet for the cell broth and to the outlet for the cell broth.
A tubular flow device (10) for enabling controlled flow of a fluid at a measuring point comprises an inlet flow channel (14) defining a main inflow direction, and an outlet flow channel (18) defining a main outflow direction. The flow device (10) further comprises a branching portion (22) where the inlet flow channel (14) is divided into a primary flow channel (24) and a secondary measurement channel (26), and a joining portion (36) where the primary flow channel (24) and the secondary measurement channel (26) open into the outlet flow channel (18). The measuring point is located in the secondary measurement channel (26). The primary flow channel (24) is longer than the secondary measurement channel (26) and has a constriction (42) downstream of the branching portion (22). A fluid entering the inlet flow channel (14) is divided into a primary flow flowing into the primary flow channel (24) and a secondary measurement flow flowing into the secondary measurement channel (26). The primary flow and the secondary measurement flow reach the joining portion (36) essentially at the same time, due to the Venturi effect caused by the constriction (42) of the primary flow channel (24).
B33Y 80/00 - Products made by additive manufacturing
G01F 1/661 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters using light
G01F 5/00 - Measuring a proportion of the volume flow
G01F 15/00 - Details of, or accessories for, apparatus of groups insofar as such details or appliances are not adapted to particular types of such apparatus
A METHOD OF PRODUCING DIFFERENTIATED CELLS FROM PLURIPOTENT STEM CELLS, CELL POPULATIONS OBTAINED BY THE METHOD, CORRESPONDING PHARMACEUTICAL COMPOSITIONS AND A SYSTEM FOR PRODUCING DIFFERENTIATED CELLS FROM PLURIPOTENT STEM CELLS
The present invention inter alia relates to a method of producing differentiated cells from pluripotent stem cells, comprising providing at least one multicellular 3D aggregate derived from pluripotent stem cells cultured in a first suspension culture, inducing differentiation of the cells forming the at least one multicellular 3D aggregate, allowing a continuous release of differentiated cells from the multicellular 3D aggregate into the first suspension culture; and continuously separating the released differentiated cells from the first suspension culture. The present invention also relates to a cell obtained by the method and to a pharmaceutical composition comprising the cells. The invention further relates to a corresponding system for producing differentiated cells from pluripotent stem cells.
A bioprocess assembly (10) comprises a separation system (12) and a sampling system (14). The sampling system (14) is fluidically connected to the separation system (12) and comprises a sampling membrane (32) such that the separation system (12) and the sampling membrane (32) provide a separate sampling channel (30). The sampling channel (30) comprises at least one fluidic connection (36) to receive a buffer and at least one fluidic connection (51) to a sampling station (50). Further, the sampling channel (30) comprises a counter pressure generating unit (48) and at least one sensor (40), wherein the counter pressure generating unit (48) is configured to adjust the pressure within the sampling channel (30) based on data collected by the at least one sensor (40).
A device assembly (10) for performing a production scale tangential flow filtration of a feed, preferably in a batch feed and bleed process, comprises a first loop (12) including a first tubing (20), a feed tank (22) and actuators and sensors adapted to the first tubing (20), and a second loop (14) including second tubing (28) and a filter (28), preferably a hollow fiber filter. The first loop (12) and the second loop (14) further include a common tubing section. The first loop (12) connects to the second loop (14) at a first connection point (16) upstream, or alternatively downstream, of the filter (28), and the second loop (14) connects to the first loop (12) at a second connection point (18) downstream, or alternatively upstream, of the filter (28), such that the first loop (12) supplies the feed from the feed tank (22) to the second loop (14) at the first connection point (16), or alternatively at the second connection point (18), and the second loop (14) supplies retentate to the first loop (12) at the second connection point (18) from where a part of the retentate is returned to the feed tank (22), or alternatively at the first connection point (14). At least all wetted parts of the device assembly (10) are single-use parts and the inner diameter of the first tubing (20) is smaller than the inner diameter of the second tubing (32).
C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
C12M 1/00 - Apparatus for enzymology or microbiology
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
Kirigami mixing systems and related methods are generally described. In some embodiments, a mixing system includes one or more grippers configured to deform a portion of a flexible container containing fluid when undergoing axial deformation. The grippers may include features including slots, hinges, and spines to aid in the transition of the grippers between a closed and retracted configuration when the one or more grippers are deformed. The compression of the container subsequently result in fluid flow within the container which may mix or agitate the fluid. In some embodiments, the described grippers may be formed integrally with the flexible containers. In some embodiments, the mixing system may include a plurality of grippers arranged around the flexible container.
B01F 31/55 - Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being contained in a flexible bag submitted to periodical deformation
B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
24.
METHOD OF OPERATING A BIOPROCESS ARRANGEMENT TO PERFORM AT LEAST ONE REPETITION OF A BIOPROCESS
The invention relates to a method of operating a bioprocess arrangement (1) to perform at least one repetition of a bioprocess, wherein the bioprocess arrangement (1) comprises at least one replaceable electronic component (8), wherein a process control system (7) controls the bioprocess arrangement (1), using a digital model (10). It is proposed that the replaceable electronic components (8) are removed and replaced by new electronic components (9), that the process control system (7) automatically detects the removal of the replaceable electronic components (8), automatically detects the presence of the new electronic components (9) and retrieves digital representations (11) of the new electronic components (9) based on the communication with the new electronic components (9), that the process control system (7) derives from the digital representations (11) of the replaceable electronic components (8) and the new electronic components (9) a functional relationship between the replaceable electronic components (8) and the new electronic components (9), that the process control system (7) replaces the digital representations (11) of the replaceable electronic components (8) with the digital representations (11) of the new electronic components (9) based on the functional relationship thereby updating the digital model (10), and, that the process control system (7) controls the bioprocess arrangement (1) to perform the bioprocess based 20 on the updated digital model (10).
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
G05B 17/02 - Systems involving the use of models or simulators of said systems electric
The invention relates to a flow-through centrifuge which is used, for example, for biotechnical applications, in particular as a blood centrifuge. A driving arrangement and/or transmission arrangement of the flow-through centrifuge comprises a planetary gearset. In the planetary gearset, at least one planetary belt pulley is rotatably supported on a rotating planet carrier. A torque of the planetary belt pulley is transmitted via a belt. The planet carrier and the planetary belt pulley are driven at different rotational speeds. According to the invention, the planet carrier is held by a belt tensioning unit rotating with the planet carrier. A distance of the planet carrier from a rotor axis can be changed via the belt tensioning unit in order to adjust the belt tension of the belt.
A valve switching system for selectively interconnecting components of a bioprocess installation, comprising a valve switching cassette and an actuator block. It is proposed, that the valve switching cassette comprises a perforated sandwich plate with perforation holes, which sandwich plate is placed between the cassette manifold and the actuator block body.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
F16K 11/22 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit operated by separate actuating members with an actuating member for each valve, e.g. interconnected to form multiple-way valves
F16K 31/126 - Operating meansReleasing devices actuated by fluid the fluid acting on a diaphragm, bellows, or the like
27.
METHOD OF OPERATING A BIOPROCESS ARRANGEMENT TO PERFORM AT LEAST ONE REPETITION OF A BIOPROCESS
A method of operating a bioprocess arrangement to perform repetition of a bioprocess, wherein the bioprocess arrangement comprises a replaceable electronic component, wherein a process control system controls the bioprocess arrangement, using a digital model. The replaceable electronic components are removed and replaced by new electronic components, the process control system automatically detects the removal of the electronic components, automatically detects the presence of the new electronic components and retrieves digital representations of the new components, the process control system derives from the digital representations of the replaceable components and the new components a functional relationship, the process control system replaces the digital representations of the replaceable components with the digital representations of the new components based on the functional relationship thereby updating the digital model, and, that the process control system controls the bioprocess arrangement to perform the bioprocess based on the updated digital model.
A method for validating a filter unit. The method includes guiding a fluid flow of a fluid after the fluid flow passed through the filter unit to a measurement area that is connected to the filter unit. The measurement area the fluid is exposed to is at least one alternating electric field between at least two electrodes. The method includes obtaining at least one electric signal between the at least two electrodes. The at least one electric signal is at least affected by whether one or more cells of a plurality of cells in the fluid pass through the measurement area. The method includes determining, at least partially based on the obtained at least one electric signal, at least one validity information indicating a validity of the filter unit.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
Chemicals and reagents for use in media and buffer
preparation, seed train, filtration, chromatography, and
virus clearance in biotechnology and biopharmaceutical
industries; chemicals for industrial and laboratory use in
biopharmaceutical processing, including those for filtration
and purification of biological materials. Machinery and automated equipment for media and buffer
preparation, seed train, filtration, chromatography, and
polishing; industrial robots and apparatus for bioprocessing
and manufacturing including filtration systems for the
purification and separation of biological substances. Scientific, research, and laboratory apparatus for use in
biopharmaceutical manufacturing, namely, apparatus for media
and buffer preparation, seed train and production,
filtration, chromatography, polishing and virus clearance;
downloadable and recorded software for biopharmaceutical
processes, namely, software for media preparation, buffer
preparation, seed train optimization, filtration,
chromatography, polishing, and virus clearance; control
systems and software for automating biopharmaceutical
processes, namely, software for filtration and purification
processes; interfaces and human-machine interface (HMI)
software for managing and optimizing biopharmaceutical
processes; data collection and management software for
process intensification systems; devices for real-time
monitoring and optimization for biopharmaceutical
production. Industrial filtration apparatus for water, air, and gas
purification; chromatography apparatus for biopharmaceutical
purification; process systems and equipment for the
filtration, purification, and separation of biological
substances in biopharmaceutical processes, namely,
filtration systems for bioprocessing. Installation and maintenance of process intensification
systems for biopharmaceutical production.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Scientific and technological research services in the field
of biopharmaceuticals, specifically relating to bioprocess
optimization, including media and buffer preparation, seed
train, filtration, chromatography, and virus clearance;
design and development of computer software for
biopharmaceutical process automation and control; technical
consultancy services related to biopharmaceutical process
optimization, focusing on bioprocess steps namely,
filtration, chromatography, and virus clearance; software as
a service (SaaS) for biopharmaceutical manufacturing,
including media and buffer preparation, seed train,
filtration, chromatography, polishing and virus clearance;
IT services in relation to cloud-based interfaces for
managing biopharmaceutical manufacturing processes, namely,
interfaces for filtration, purification, and virus clearance
systems; testing and validation services for bioprocessing
equipment and systems; process scale-up technical consulting
and technical support for biopharmaceutical production.
Various embodiments provide a method for producing a bioproduct using a bioprocess installation. The bioprocess installation comprises an process control and a bioprocess unit. The bioprocess unit comprises a receptacle, a clarification unit with a centrifuge and a chromatography unit with a chromatograph. Cell broth obtained from the receptacle is lead through the clarification unit and the chromatography unit in a liquid stream. The clarification unit with its centrifuge is being operated in a centrifugation cycle comprising centrifugation steps. The chromatography unit with its chromatograph is being operated in a chromatography cycle comprising chromatography steps. The particle depletion can be less than 10% in particle concentration and that the execution of the steps assigned to the centrifugation cycle and the execution of the steps assigned to the chromatography cycle are at least partly being synchronized with each other by the process control in synchronization routines based on assigned synchronization strategies.
Systems and methods for characterizing a physical and solution properties of liquids are described. In some embodiments, an ultrasonic interrogation signal may be emitted into a liquid such as a solubilized protein solution. A resulting ultrasonic spectrum may be sensed and provided to a trained statistical model of the solution. The trained statistical model may then determine one or more properties of the liquid. In some embodiments, the trained statistical model determines a viscosity of the liquid and/or a solubilized protein concentration of the liquid. In some embodiments, a trained statistical classification model configured to classify the liquid based, for example, on its contents or properties is used to improve modeling accuracy.
The invention relates to a centrifuge, in particular a continuous flow centrifuge, a biotechnical centrifuge or a blood centrifuge. The centrifuge comprises a refrigerant circuit. According to the invention, the outlet side of a controllable compressor is connected to an inlet side of an evaporator via a bypass line with a valve arranged therein. In a normal operation mode, a centrifuge vessel is cooled exclusively via the control of the compressor and an expansion unit while the valve is closed. If, on the other hand, a tolerance range of the target temperature in the centrifuge vessel is left, the valve is opened in order to bring about heating and thus a return of the temperature in the centrifuge vessel to the tolerance range.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Filter modules, namely, filter capsules, filter candles, filter cassettes, coil modules, filter coils, filter coils in connection with filter centrifuges, band filters, small filters, small filters in connection with centrifuge tubes and as centrifugal concentrators, disposable filters; Membrane filters and filter matting being parts of machines, for use in biotechnology, environmental protection, for the drinks, food, luxury items, pharmaceutical, cosmetics, electronics and general chemical industries, and for use in genetic engineering, medicine and in laboratories, and for treatment and analysis of water and effluent; Filters being parts of machines; Membrane filters for use as part of machines. Laboratory filters; Filtering units for laboratory use; Liquid filter units, namely, filter cartridges, plastic hoses and hose connectors sold as a set for laboratory use; Membranes for filtration [scientific]; Filters for use with laboratory chromatography apparatus; Membrane filters and filter matting with adsorption properties, membrane adsorbers, namely adsorption membranes, ion exchange membranes, ligand membranes and activated membranes in the form of hollow fibre membranes, tube membranes and flat membranes as strips or cut to size for use in apparatus, filters and filter modules for the aforesaid fields of application; Membrane filters and filter matting as parts of microtitre plates; Membrane filters and filter matting as parts of diagnostic tests such as diagnostic assays and immunoassays; Membrane filters and filter matting as parts of filter systems or as independent filters for chromatographic analyses and chemical separation, reconcentration, for sterilisation and ultrapurification of fluids; Filters and filter modules for filtering and reconcentration of biological solutions, namely of viral and bacterial suspensions; Filters and filter modules for treating blood and other bodily fluids. Filters and filtering units, for industrial use; Filters for filtering fluids, filter modules, filter candles and filter capsules; Liquid filter units, namely, filter cartridges, plastic hoses and hose connectors, sold as a set, for industrial installations; Filters for water filtering apparatus; Cartridge filtration units [water treatment equipment]; Filters for use with industrial chromatography apparatus; Apparatus for water treatment and for preparing solutions in the pharmaceutical, medical and laboratory fields; Equipment for de-ionising water; Equipment for de-pyrogenisation of solutions and for separating harmful substances from fluids; Filters and filter modules for desalination of protein solutions and other biological media; Filters and filter modules for analysis and separation of ions, macromolecules and biomolecules, namely carbohydrates, peptides, proteins and nucleic acids and for separating heavy metals and harmful substances from fluids; Filters for industrial use, namely, membrane filters and filter matting having applications in biotechnology, environmental protection, the drinks, food, luxury items, pharmaceutical, cosmetics, electronics and general chemical industries, and in genetic engineering, medicine and laboratories, and the treatment and analysis of water and effluents.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Scientific and technological research services in the field of biopharmaceuticals, specifically relating to bioprocess optimization, including media and buffer preparation, seed train, filtration, chromatography, and virus clearance; design and development of computer software for biopharmaceutical process automation and control; technical consultancy services related to biopharmaceutical process optimization, focusing on bioprocess steps namely, filtration, chromatography, and virus clearance; software as a service (SaaS) for biopharmaceutical manufacturing, including media and buffer preparation, seed train, filtration, chromatography, polishing and virus clearance; IT services in relation to cloud-based interfaces for managing biopharmaceutical manufacturing processes, namely, interfaces for filtration, purification, and virus clearance systems; testing and validation services for bioprocessing equipment and systems; process scale-up technical consulting and technical support for biopharmaceutical production.
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Scientific and technological research services in the field of biopharmaceuticals, specifically relating to bioprocess optimization, including media and buffer preparation, seed train, filtration, chromatography, and virus clearance; design and development of computer software for biopharmaceutical process automation and control; technical consultancy services related to biopharmaceutical process optimization, focusing on bioprocess steps namely, filtration, chromatography, and virus clearance; software as a service (SaaS) for biopharmaceutical manufacturing, including media and buffer preparation, seed train, filtration, chromatography, polishing and virus clearance; IT services in relation to cloud-based interfaces for managing biopharmaceutical manufacturing processes, namely, interfaces for filtration, purification, and virus clearance systems; testing and validation services for bioprocessing equipment and systems; process scale-up technical consulting and technical support for biopharmaceutical production.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
(1) Chemicals and reagents for use in media and buffer preparation, seed train, filtration, chromatography, and virus clearance in biotechnology and biopharmaceutical industries; chemicals for industrial and laboratory use in biopharmaceutical processing, including those for filtration and purification of biological materials.
(2) Machinery and automated equipment for media and buffer preparation, seed train, filtration, chromatography, and polishing; industrial robots and apparatus for bioprocessing and manufacturing including filtration systems for the purification and separation of biological substances.
(3) Scientific, research, and laboratory apparatus for use in biopharmaceutical manufacturing, namely, apparatus for media and buffer preparation, seed train and production, filtration, chromatography, polishing and virus clearance; downloadable and recorded software for biopharmaceutical processes, namely, software for media preparation, buffer preparation, seed train optimization, filtration, chromatography, polishing, and virus clearance; control systems and software for automating biopharmaceutical processes, namely, software for filtration and purification processes; interfaces and human-machine interface (HMI) software for managing and optimizing biopharmaceutical processes; data collection and management software for process intensification systems; devices for real-time monitoring and optimization for biopharmaceutical production.
(4) Industrial filtration apparatus for water, air, and gas purification; chromatography apparatus for biopharmaceutical purification; process systems and equipment for the filtration, purification, and separation of biological substances in biopharmaceutical processes, namely, filtration systems for bioprocessing. (1) Installation and maintenance of process intensification systems for biopharmaceutical production.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
Chemicals and reagents for use in media and buffer preparation, seed train, filtration, chromatography, and virus clearance in biotechnology and biopharmaceutical industries; chemicals for industrial and laboratory use in biopharmaceutical processing, including those for filtration and purification of biological materials. Machinery and automated equipment for media and buffer preparation, seed train, filtration, chromatography, and polishing; industrial robots and apparatus for bioprocessing and manufacturing including filtration systems for the purification and separation of biological substances. Scientific, research, and laboratory apparatus for use in biopharmaceutical manufacturing, namely, apparatus for media and buffer preparation, seed train and production, filtration, chromatography, polishing and virus clearance; downloadable and recorded software for biopharmaceutical processes, namely, software for media preparation, buffer preparation, seed train optimization, filtration, chromatography, polishing, and virus clearance; control systems and software for automating biopharmaceutical processes, namely, software for filtration and purification processes; interfaces and human-machine interface (HMI) software for managing and optimizing biopharmaceutical processes; data collection and management software for process intensification systems; devices for real-time monitoring and optimization for biopharmaceutical production. Industrial filtration apparatus for water, air, and gas purification; chromatography apparatus for biopharmaceutical purification; process systems and equipment for the filtration, purification, and separation of biological substances in biopharmaceutical processes, namely, filtration systems for bioprocessing. Installation and maintenance of process intensification systems for biopharmaceutical production.
A device assembly including a single-use device for sensing or influencing a parameter of a running bioprocess. The single-use device is configured to have at least two different defined functional states, a first functional state being an inactive delivery state in which the single-use device is sterile and inoperable according to its intended use, and a second functional state being an active use state in which the single-use device is operable according to its intended use. The device assembly further includes a signalling element for indicating a current functional state of the single-use device to a user. Each functional state is associated with a distinct signal. The device assembly is configured such that a transfer from the first functional state to any other functional state of the single-use device is irreversible and/or permanently prevents the signalling element from indicating the signal associated with the first functional state.
A method of harvesting cell broth (12) from a mixing device (10) for mixing the cell broth (12) of a rocking motion bioreactor is shown. The mixing device (10) comprises a rocking motion platform (14), a mixing bag (18) and a processing unit (22). After a harvest process is started, the mixing bag (18) is tilted into a default harvest position and the cell broth (12) is harvested in the default harvest position of the mixing bag (18). If a pause condition is fulfilled, harvesting is paused and the mixing device starts rocking at a defined speed and a defined rocking angle for a predetermined time. After the predetermined time, harvesting in the default harvest position is resumed. Furthermore, a mixing device (10) for mixing cell broth (12) is disclosed.
A method of controlling a biopharmaceutical purification process, preferably a continuous process, making use of a separation arrangement. The separation arrangement preferably includes multiple chromatography columns or filtration devices. The method comprises the following steps: repeatedly acquiring an absorption spectrum inline or online at a fluid stream with a detector which is capable of recording spectra within a wavelength range between 190 nm and 2400 nm, preferably between 190 nm and 390 nm or 190 nm and 780 nm or 1100 nm and 2400 nm; real-time processing of the acquired spectrum; and real- time monitoring and controlling the process based on the processed spectrum. The step of processing includes at least one of the following measures: reducing noise; reducing spectral interference; correcting the acquired spectrum for scattering effects and/or other background effects.
The disclosure relates to monitoring or controlling bioprocesses by way of withdrawing discrete fluid samples from a reactor and performing on-line analysis of said sample using a fluidic manifold, for example, by way of a sensor based on label-free biomolecular interaction analysis. The disclosure relates to a system for measuring one or more analytes in discrete fluid samples from a vessel adapted to contain a fluid having one or more sensors for the measurement of the analytes in a contact volume of the fluid sample. An analysis module having at least one main sampling line in fluidic connection with the vessel and one or more pumps in fluidic connection with said main sampling line(s) and adapted to selectively pump fluid from in the main sampling line away from the vessel. At least one fluid distribution tubing in fluidic connection with the analysis module. A manifold has a body.
The invention relates to a method for automating the foam regulation during a bioprocess for cultivating cells in a bioreactor, said method comprising the following steps: determining values of one or more process variables of the bioprocess which is/are relevant for the formation of foam in the bioreactor; transferring the determined values as input signals to a controller; generating, by the controller, output signals for at least one foam regulation device depending on the input signals; and controlling the at least one foam regulation device in a requirements-oriented manner by means of the output signals output by the controller.
A coupling device (1) for coupling to a flexible diaphragm (51) of a diaphragm device (50) and comprising a base structure (10) for attaching the diaphragm device (50) thereto; an actuator structure (20) mounted movable along an actuation direction (A) relative to the base structure (10) and having a diaphragm grasping element (22) pivotably mounted between a release position and a grasping position for coupling the actuator structure (20) to the flexible diaphragm (51) in the grasping position; and a fixation structure (30) mounted movable along a fixation direction (F) relative to the base structure (10) between an open position and a closed position and designed to fix in the closed position the diaphragm grasping element (22) against leaving the grasping position.
F16K 1/48 - Attaching valve members to valve-spindles
F16K 7/16 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with flat, dished, or bowl-shaped diaphragm arranged to be deformed against a flat seat the diaphragm being mechanically actuated, e.g. by screw-spindle or cam
F16L 37/127 - Couplings of the quick-acting type in which the connection between abutting or axially-overlapping ends is maintained by locking members using hooks, pawls, or other movable or insertable locking members using hooks hinged about an axis
A membrane for microbiological analysis, a production method of a membrane for microbiological analysis, and the use of such membranes for microbiological analysis. Examples include a cellulose membrane for microbiological analysis that is impregnated with a non-ionic surfactant in an amount of from 100 ng/cm2 to 1.0 mg/cm2, with the membrane having a nominal pore size of from 0.20 μm to 0.80 μm, and a cumulative adsorption pore volume of less than 0.010 cm3/g.
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
17 - Rubber and plastic; packing and insulating materials
Goods & Services
Filter modules, namely filter capsules, filter candles,
filter cassettes, coil modules, filter coils, filter coils
in connection with filter centrifuges, band filters, small
filters, small filters in connection with centrifuge tubes
and as centrifugal concentrators, disposable filters;
membrane filters and filter matting being parts of machines,
for use in biotechnology, environmental protection, for the
drinks, food, luxury items, pharmaceutical, cosmetics,
electronics and general chemical industries, and for use in
genetic engineering, medicine and in laboratories, and for
treatment and analysis of water and effluent; filters being
parts of machines; membrane filters for use as part of
machines. Laboratory filters; filtering units for laboratory use;
liquid filter units, namely, filter cartridges, plastic
hoses and hose connectors sold as a set for laboratory use;
membranes for filtration (laboratory use); filters for use
with laboratory chromatography apparatus; membrane filters
and filter matting for laboratories with adsorption
properties, membrane adsorbers, namely adsorption membranes,
ion exchange membranes, ligand membranes and activated
membranes in the form of hollow fibre membranes, tube
membranes and flat membranes as strips or cut to size for
use in apparatus, filters and filter modules for the
aforesaid fields of application; membrane filters and filter
matting as parts of microtitre plates; membrane filters and
filter matting as parts of diagnostic tests such as
diagnostic assays and immunoassays; membrane filters and
filter matting as parts of filter systems or as independent
filters for chromatographic analyses and chemical
separation, reconcentration, for sterilisation and
ultrapurification of fluids; filters and filter modules for
filtering and reconcentration of biological solutions,
namely of viral and bacterial suspensions; filters and
filter modules for treating blood and other bodily fluids. Air filters and filtering units, for industrial use; liquid
filters and filtering units, for industrial use; filters for
filtering fluids, filter modules, filter candles and filter
capsules; liquid filter units, namely, filter cartridges,
plastic hoses and hose connectors, sold as a set, for
industrial installations; filters for water filtering
apparatus; cartridge filtration units [water treatment
equipment]; filters for use with industrial chromatography
apparatus; apparatus for water treatment and for preparing
solutions in the pharmaceutical, medical and laboratory
fields; equipment for de-ionising water; equipment for
de-pyrogenisation of solutions and for separating harmful
substances from fluids; filters and filter modules for
desalination of protein solutions and other biological
media; filters and filter modules for analysis and
separation of ions, macromolecules and biomolecules, namely
carbohydrates, peptides, proteins and nucleic acids and for
separating heavy metals and harmful substances from fluids;
filters for industrial use, namely, membrane filters and
filter matting having applications in biotechnology,
environmental protection, the drinks, food, luxury items,
pharmaceutical, cosmetics, electronics and general chemical
industries, and in genetic engineering, medicine and
laboratories, and the treatment and analysis of water and
effluents. Membranes and semi-processed synthetic filtering materials;
polymeric membranes; polymeric porous membranes in hollow
fibre form; polymeric porous membranes in sheet form;
transfer membranes of cellulose derivatives; transfer
membranes of cellulose; transfer membranes of polyamides;
transfer membranes of polyvinylidene fluoride; cross-linked
polymeric membranes in sheet form; cross-linked polymeric
membranes in hollow fibre form.
47.
METHOD FOR PERFORMING A TANGENTIAL FLOW FILTRATION ON A BIOMOLECULAR SOLUTION
The invention relates to a method for performing a tangential flow filtration on a biomolecular solution, in particular for performing a single pass tangential flow filtration on a biomolecular solution, as part of a production process for biomolecules, wherein a tangential flow filtration arrangement (1) is provided, wherein the tangential flow filtration arrangement (1) comprises a tangential flow filtration module (2), wherein the tangential flow filtration is performed inside the tangential flow filtration module (2), wherein the tangential flow filtration module (2) comprises a filter (3), a feed input fluid line (4) for the biomolecular solution as a feed medium, a retentate output fluid line (6) for a retentate, a filtrate output fluid line (7) for a filtrate, and preferably a buffer input fluid line (5) for a buffer, wherein the tangential flow filtration arrangement (1) comprises at least two actuators (8) influencing the tangential flow filtration, wherein the tangential flow filtration arrangement (1) comprises a control module (10), wherein the control module (10) controls the tangential flow filtration by providing control signals to the actuators (8) and measuring at least two measured variables (11) describing the tangential flow filtration, wherein the control module (10) uses a process control to control the tangential flow filtration. It is proposed that the process control is a model predictive control (13), that the control module (10) inputs the measured variables (11) as input variables (14) into the model and derives at least two manipulated variables (15) as control signals for the actuators (8).
The invention relates to a device for continuous virus inactivation during a protein production process, in particular an antibody production process, wherein the device comprises an axially extending hollow-body holder (1) for holding an elongated, elastically deformable hollow body (2), in particular a flexible tube, wherein the device comprises at least two axially extending rollers (3, 4), in particular three or four rollers (3, 4, 5, 6), each of which, when the device has been assembled as intended, squeezes the hollow body (2) and thereby divides the internal volume (7) of the hollow body (2) into volume portions (8) fluidically separated from one another, wherein the continuous virus inactivation is carried out in the volume portions (8) of the hollow body (2). It is proposed that the device comprises a support element (9) which, when the device has been assembled as intended, supports the lateral faces of each of the rollers (3, 4, 5, 6) in at least one point.
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
A61L 2/00 - Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lensesAccessories therefor
C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
C12M 1/00 - Apparatus for enzymology or microbiology
B01D 15/24 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
49.
METHOD OF OPERATING A BIOPROCESSING ARRANGEMENT COMPRISING A CLARIFICATION SET-UP TO REMOVE CELL DEBRIS FROM A CELL BROTH
The invention relates to a method of operating a bioprocessing arrangement (1) comprising a clarification set-up (2) to remove cell debris from a cell broth (10), wherein the clarification set-up (2) comprises a fluidized bed centrifuge (8) with at least one centrifuge chamber (11), wherein the cell broth (10) is separated into at least a waste fraction (12) and an output fraction (13), wherein the separation of the cell broth (10) into the waste fraction (12) and the output fraction (13) comprises a loading step (14) during which the centrifuge chamber (11) is loaded with cells of the cell broth (10) up to a capacity below or equal to a maximum cell loading capacity of the centrifuge chamber (11), an overloading step (15) during which the centrifuge chamber (11) is further loaded with cell broth (10), thereby flushing out cells from the centrifuge chamber (11), collecting cells flushed out during the overloading step (15) as the waste fraction (12), and collecting cells from the chamber as the output fraction (13).
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; filtration membranes for laboratory use;
laboratory filtration apparatus and filtering materials for
the filtration of liquids and gases; filters, for use in
relation to the following goods: laboratory apparatus in the
biopharmaceutical industry; filtration systems, devices, and
membranes for laboratory use. Filters for industrial use, namely for liquids, gases, and
biopharmaceutical applications; filtration units; filtration
units, namely filter cartridges, filter capsules, filter
modules, filter capsules and disposable filters, for
industrial purposes; filters, namely small filters,
disposable filters, filter cartridges, filter modules and
diaphragm filters being parts of filtering units or being
independent filters, for sterilisation and purification of
liquids and gases in the beverage, food and general chemical
industry; filters, specifically filter cartridges and
membrane filters for single use as parts of filter systems
or as stand-alone filters for the sterilization and
purification of liquids and gases in the electronics,
pharmaceutical, food, beverage, luxury food, cosmetics and
general chemical industries.
51.
SEPARATION SYSTEM AND METHOD FOR SEPARATING AND PURIFYING A TARGET COMPONENT
A separation system for separating and purifying a target component, a method for separating and purifying a target component, and the use of a crossflow diafiltration unit for processing a target phase. The system includes an aqueous-two phase extraction unit for aqueous-two phase extraction of the fluid, a separation unit for separating a target phase, and a crossflow diafiltration unit.
A smart single-use tube line assembly for use in a unit operation of a bioprocess including at least one tube line. The tube line includes a tube body surrounding a lumen through which a medium can flow. The tube line assembly also includes a functional element embedded in the tube body, the functional element being configured to perform an optical and/or electrical function. The tube line assembly further includes an interface for coupling both the lumen and the functional element of the tube line to another component. The tube line assembly still further includes a control unit for controlling the optical and/or electrical function.
The invention relates to a an aeration device for a bioprocessing installation (2), comprising a housing (4), a circumferential rigid body (5), wherein the housing (4) comprises at least a first aeration channel (7) and a second aeration channel (8), wherein the housing (4) further comprises at least a first gas inlet port (10) and a second gas inlet port (11), and wherein the housing (4) further comprises a plurality of first gas discharge openings (12) and second gas discharge openings (13). It is proposed that the first aeration channel (7) and the second aeration channel (8) are arranged so as to overlap one another in the axial direction at least in sections.
A clamping device for fluidically and mechanically connecting two hose barbs. The clamping device includes a seal and a locking mechanism, wherein the locking mechanism can be transferred from a mounting condition to an assembled condition. In the mounting condition, the hose barbs can be inserted into the clamping device, while in the assembled condition, those hose barbs are locked in place in a position in which free ends of the hose barbs face each other and a seal coaxially aligns and fluid-tightly connects the free ends.
F16L 33/22 - Arrangements for connecting hoses to rigid membersRigid hose-connectors, i.e. single members engaging both hoses with means not mentioned in the preceding groups for gripping the hose between inner and outer parts
F16L 33/18 - Arrangements for connecting hoses to rigid membersRigid hose-connectors, i.e. single members engaging both hoses characterised by the use of additional sealing means
55.
FILTRATION DEVICE, IN PARTICULAR SMALL-SCALE FILTRATION DEVICE FOR PRODUCTION OF BIOPHARMACEUTICALS
A filtration device (10), in particular a small-scale filtration device for production of biopharmaceuticals, comprises a housing including a top cover (12) having an unfiltrate inlet (18) and a bottom cover (14) having a filtrate outlet (22), and one or more filter supports (16) stacked between the top cover (12) and the bottom cover (14) along a vertical direction such that an upper side of each filter support (16) faces the top cover (12) and a lower side of each filter support (16) faces the bottom cover (14). Each filter support (16) is equipped with a flat upper filter (40) and a flat lower filter (42). Each filter (40, 42) has a filtrate side and an opposite unfiltrate side. The upper and lower filters (40, 42) are sealed to the upper and lower sides of the filter support (16), respectively, with the filtrate sides facing the filter support (16) and the unfiltrate sides facing away from the filter support (16). Unfiltrate flow paths lead from the unfiltrate inlet of the top cover (12) to the unfiltrate side of the upper filter (40) and to the unfiltrate side of the lower filter (42), wherein blocking means prevent unfiltrate from flowing directly from the unfiltrate inlet of the top cover (12) to the filtrate outlet (22) of the bottom cover (14). Filtrate flow paths lead from the filtrate sides of the upper and lower filters (40, 42) to the filtrate outlet (22) of the bottom cover (14) via at least one filtrate guiding channel (48a, 48b) formed on the upper side of the filter support (16) and at least one filtrate guiding channel (48a, 48b) formed on the lower side of the filter support (16). The upper filter (40) and the lower filter (42) are identically shaped.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; filtration membranes for laboratory use; laboratory filtration apparatus and filtering materials for the filtration of liquids and gases; filters, for use in relation to the following goods: laboratory apparatus in the biopharmaceutical industry; filtration systems, devices, and membranes for laboratory use. Filters for industrial use, namely for liquids, gases, and biopharmaceutical applications; filtration units; filtration units, namely filter cartridges, filter capsules, filter modules, filter capsules and disposable filters, for industrial purposes; filters, namely small filters, disposable filters, filter cartridges, filter modules and diaphragm filters being parts of filtering units or being independent filters, for sterilisation and purification of liquids and gases in the beverage, food and general chemical industry; filters, specifically filter cartridges and membrane filters for single use as parts of filter systems or as stand-alone filters for the sterilization and purification of liquids and gases in the electronics, pharmaceutical, food, beverage, luxury food, cosmetics and general chemical industries.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
(1) Laboratory filters; filtration membranes for laboratory use; laboratory filtration apparatus and filtering materials for the filtration of liquids and gases; filters, for use in relation to the following goods: laboratory apparatus in the biopharmaceutical industry; filtration systems, devices, and membranes for laboratory use.
(2) Filters for industrial use, namely for liquids, gases, and biopharmaceutical applications; filtration units; filtration units, namely filter cartridges, filter capsules, filter modules, filter capsules and disposable filters, for industrial purposes; filters, namely small filters, disposable filters, filter cartridges, filter modules and diaphragm filters being parts of filtering units or being independent filters, for sterilisation and purification of liquids and gases in the beverage, food and general chemical industry; filters, specifically filter cartridges and membrane filters for single use as parts of filter systems or as stand-alone filters for the sterilization and purification of liquids and gases in the electronics, pharmaceutical, food, beverage, luxury food, cosmetics and general chemical industries.
58.
METHOD AND SYSTEM FOR TESTING THE INTEGRITY OF FILTERS
A method for testing integrity of a filter can include pressurizing an upstream side of the filter to a test pressure and performing a check step that includes determining a flow rate of fluid from the upstream side to a downstream side of the filter, comparing the determined flow rate with a flow range including a flow threshold, and setting stop criteria based on the comparison.
The present invention relates to a method for treating an ultrafiltration membrane, a method for producing an ultrafiltration membrane comprising the treatment method, as well as ultrafiltration membrane obtained by the treatment method or the production method.
A modular processing system (100) for biopharmaceutical processes includes (i) at least one first and at least one second processing unit (30, 32), which can be fluidically connected to each other; and (ii) at least one adapter plate (200), through which at least one fluid flow (14), flowing from the first processing unit (30) to the second processing unit (32), can flow. The adapter plate deflects the fluid flow between the first processing unit and the second processing unit at least partially; and/or controls the fluid flow, preferably, the pressure thereof, in an open loop or closed loop manner. A method for the modular construction of a processing system (100) for biopharmaceutical processes is also disclosed.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
Chemicals and reagents for use in media and buffer preparation, seed train, filtration, chromatography, and virus clearance in biotechnology and biopharmaceutical industries; Chemicals for industrial and laboratory use in biopharmaceutical processing, including those for filtration and purification of biological materials. Machinery and automated equipment for media and buffer preparation, seed train, filtration, chromatography, and polishing; Industrial robots and apparatus for bioprocessing and manufacturing including filtration systems for the purification and separation of biological substances. Scientific, research, and laboratory apparatus for use in biopharmaceutical manufacturing, namely, apparatus for media and buffer preparation, seed train and production, filtration, chromatography, polishing and virus clearance; Downloadable and recorded software for biopharmaceutical processes, namely, software for media preparation, buffer preparation, seed train optimization, filtration, chromatography, polishing, and virus clearance; Control systems and software for automating biopharmaceutical processes, namely, software for filtration and purification processes; Interfaces and human-machine interface (HMI) software for managing and optimizing biopharmaceutical processes; Data collection and management software for process intensification systems; Devices for real-time monitoring and optimization for biopharmaceutical production. Industrial filtration apparatus for water, air, and gas purification; Chromatography apparatus for biopharmaceutical purification; Process systems and equipment for the filtration, purification, and separation of biological substances in biopharmaceutical processes, namely, filtration systems for bioprocessing. Installation and maintenance of process intensification systems for biopharmaceutical production.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Scientific and technological research services in the field of biopharmaceuticals, specifically relating to bioprocess optimization, including media and buffer preparation, seed train, filtration, chromatography, and virus clearance; Design and development of computer software for biopharmaceutical process automation and control; Technical consultancy services related to biopharmaceutical process optimization, focusing on bioprocess steps namely, filtration, chromatography, and virus clearance; Software as a service (SaaS) for biopharmaceutical manufacturing, including media and buffer preparation, seed train, filtration, chromatography, polishing and virus clearance; IT-services in relation to cloud-based interfaces for managing biopharmaceutical manufacturing processes, namely, interfaces for filtration, purification, and virus clearance systems; Testing and validation services for bioprocessing equipment and systems; Process scale-up technical consulting and technical support for biopharmaceutical production.
The invention relates to a portable container holder for use in a bioprocess for holding and transporting a disposable container (3) which is filled with a fluid (2) and which has received a magnetically driven mixing element (4) for mixing the fluid (2), wherein the portable container holder (1) has a container receiving area (7) for receiving the disposable container (3), said receiving area having a drive location (8) where the mixing element (4) can be arranged in the disposable container (3) received in the container receiving area (7) such that the mixing element (4) can be driven by a drive unit (9) at the drive location (8). According to the invention, the portable container holder (1) has a transport securing unit (11) for securing and releasing the mixing element (4), which is arranged at the drive location (8), in its position, and the transport securing unit (11) has a magnet (10) which can be moved between a defined securing position (12), in which the mixing element (4) is secured in its position, and a defined release position (13), in which the mixing element (4) is released from its position.
The invention relates to a portable container holder for use in a bioprocess for holding and transporting a flexible disposable container (3) which is filled with a fluid (2). In particular, the fluid (2) is a fluid (2) which contains a purified pharmaceutical ingredient or a cultivation medium, and the portable container holder (1) has a container receiving area (6) for receiving the disposable container (3) and a cover (7) which can be installed on the container receiving area (6) in order to cover (7) the disposable container (3) received in the container receiving area (6), wherein the cover (7) has a fixing assembly (8) via which the disposable container (3) filled with the fluid (2) can be fixed in the container receiving area (6) in the installed state of the cover (7). According to the invention, the cover (7) has a spring assembly (9) which elastically supports the fixing assembly (8), and a spring force can be applied to the fixing assembly (8) in the direction of the spring via the spring assembly (9) in the installed state of the cover (7).
A device arrangement for separating cells from a culture medium in a bioprocess, in particular in a biopharmaceutical process, including a storage tank for the culture medium; a filtration module having a membrane adapted to be overflowed; an intake line connecting the storage tank to an unfiltrate inlet for supplying culture medium into the filtration module; a return line which connects a retentate-side outlet of the filtration module to the storage tank for returning retentate into the storage tank; a filtrate line which is connected to a permeate-side outlet of the filtration module for discharging filtrate; a first pump for maintaining a recirculation circuit; a backflush line which leads to a permeate-side connection and/or to the permeate-side outlet for supplying a rinsing liquid into the filtration module; and a second pump for conveying the rinsing liquid into the filtration module. A method of separating cells from a culture medium.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
17 - Rubber and plastic; packing and insulating materials
Goods & Services
Filter modules, namely filter capsules, filter candles, filter cassettes, coil modules, filter coils, filter coils in connection with filter centrifuges, band filters, small filters, small filters in connection with centrifuge tubes and as centrifugal concentrators, disposable filters; membrane filters and filter matting being parts of machines, for use in biotechnology, environmental protection, for the drinks, food, luxury items, pharmaceutical, cosmetics, electronics and general chemical industries, and for use in genetic engineering, medicine and in laboratories, and for treatment and analysis of water and effluent; filters being parts of machines; membrane filters for use as part of machines. Laboratory filters; filtering units for laboratory use; liquid filter units, namely, filter cartridges, plastic hoses and hose connectors sold as a set for laboratory use; membranes for filtration (laboratory use); filters for use with laboratory chromatography apparatus; membrane filters and filter matting for laboratories with adsorption properties, membrane adsorbers, namely adsorption membranes, ion exchange membranes, ligand membranes and activated membranes in the form of hollow fibre membranes, tube membranes and flat membranes as strips or cut to size for use in apparatus, filters and filter modules for the aforesaid fields of application; membrane filters and filter matting as parts of microtitre plates; membrane filters and filter matting as parts of diagnostic tests such as diagnostic assays and immunoassays; membrane filters and filter matting as parts of filter systems or as independent filters for chromatographic analyses and chemical separation, reconcentration, for sterilisation and ultrapurification of fluids; filters and filter modules for filtering and reconcentration of biological solutions, namely of viral and bacterial suspensions; filters and filter modules for treating blood and other bodily fluids. Air filters and filtering units, for industrial use; liquid filters and filtering units, for industrial use; filters for filtering fluids, filter modules, filter candles and filter capsules; liquid filter units, namely, filter cartridges, plastic hoses and hose connectors, sold as a set, for industrial installations; filters for water filtering apparatus; cartridge filtration units [water treatment equipment]; filters for use with industrial chromatography apparatus; apparatus for water treatment and for preparing solutions in the pharmaceutical, medical and laboratory fields; equipment for de-ionising water; equipment for de-pyrogenisation of solutions and for separating harmful substances from fluids; filters and filter modules for desalination of protein solutions and other biological media; filters and filter modules for analysis and separation of ions, macromolecules and biomolecules, namely carbohydrates, peptides, proteins and nucleic acids and for separating heavy metals and harmful substances from fluids; filters for industrial use, namely, membrane filters and filter matting having applications in biotechnology, environmental protection, the drinks, food, luxury items, pharmaceutical, cosmetics, electronics and general chemical industries, and in genetic engineering, medicine and laboratories, and the treatment and analysis of water and effluents. Membranes and semi-processed synthetic filtering materials; polymeric membranes; polymeric porous membranes in hollow fibre form; polymeric porous membranes in sheet form; transfer membranes of cellulose derivatives; transfer membranes of cellulose; transfer membranes of polyamides; transfer membranes of polyvinylidene fluoride; cross-linked polymeric membranes in sheet form; cross-linked polymeric membranes in hollow fibre form.
68.
PRODUCT FOR PROVIDING MEMBRANE ELEMENTS AND MANUFACTURING METHOD
The invention relates to a product for providing membrane elements, comprising: a carrier film; and a membrane film, at least comprising: a support layer; and a membrane layer; the membrane film is releasably coupled to the carrier film; and the membrane film has a plurality of substantially discrete membrane elements. The invention also relates to a corresponding manufacturing process.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B32B 3/30 - Layered products comprising a layer with external or internal discontinuities or unevennesses, or a layer of non-planar shapeLayered products comprising a layer having particular features of form characterised by a particular shape of the outline of the cross-section of a continuous layerLayered products comprising a layer with external or internal discontinuities or unevennesses, or a layer of non-planar shapeLayered products comprising a layer having particular features of form characterised by a layer with cavities or internal voids characterised by a layer formed with recesses or projections, e.g. grooved, ribbed
B32B 7/06 - Interconnection of layers permitting easy separation
B32B 7/12 - Interconnection of layers using interposed adhesives or interposed materials with bonding properties
B32B 23/06 - Layered products essentially comprising cellulosic plastic substances comprising such substance as the main or only constituent of a layer, next to another layer of a specific substance of paper or cardboard
B32B 23/08 - Layered products essentially comprising cellulosic plastic substances comprising such substance as the main or only constituent of a layer, next to another layer of a specific substance of synthetic resin
B32B 27/08 - Layered products essentially comprising synthetic resin as the main or only constituent of a layer next to another layer of a specific substance of synthetic resin of a different kind
B32B 27/10 - Layered products essentially comprising synthetic resin as the main or only constituent of a layer next to another layer of a specific substance of paper or cardboard
B32B 27/28 - Layered products essentially comprising synthetic resin comprising copolymers of synthetic resins not wholly covered by any one of the following subgroups
B32B 37/12 - Methods or apparatus for laminating, e.g. by curing or by ultrasonic bonding characterised by using adhesives
B32B 37/18 - Methods or apparatus for laminating, e.g. by curing or by ultrasonic bonding characterised by the properties of the layers with all layers existing as coherent layers before laminating involving the assembly of discrete sheets or panels only
B32B 38/10 - Removing layers, or parts of layers, mechanically or chemically
69.
BIOPROCESS SYSTEM FOR A BIOPROCESS OPERATION WITH A DISPLAY ARRANGEMENT FOR DISPLAYING SYSTEM INFORMATION ABOUT THE BIOPROCESS SYSTEM
The invention relates to a bioprocess system for a bioprocess operation with a display arrangement (21) for displaying system information about the bioprocess system (1), wherein the bioprocess system (1) comprises system components (6) comprising at least one monitored system component (22), wherein the system components (6) can be assembled into an assembled state in which the bioprocess system (1) is operational for the bioprocess operation and can be disassembled into a disassembled state in which the bioprocess system (1) is not operational for the bioprocess operation, wherein the bioprocess system (1) comprises a control arrangement (24) and the display arrangement (21) comprises at least one display component (25), wherein in an assembled state of the bioprocess system (1) during a bioprocess operation the control arrangement (24) displays process information via the display arrangement (21) and/or wherein during assembly of the bioprocess system (1) the control arrangement (24) displays assembly information via the display arrangement (21), wherein the process information and/or the assembly information concern at least the monitored system component (22) and wherein the display arrangement (21) comprises at least one display component (25). It is proposed that the display component (25) comprises a light emitting element (27) arranged separate from the monitored system component (22) and arranged next to or on the monitored system component (22) and that the control arrangement (24) is configured to display via the light emitting element (27) the process information and/or the assembly information relating to the monitored system component (22).
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G05B 19/409 - Numerical control [NC], i.e. automatically operating machines, in particular machine tools, e.g. in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form characterised by using manual data input [MDI] or by using control panel, e.g. controlling functions with the panelNumerical control [NC], i.e. automatically operating machines, in particular machine tools, e.g. in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form characterised by control panel details or by setting parameters
G06F 9/448 - Execution paradigms, e.g. implementations of programming paradigms
G05B 19/40 - Open loop systems, e.g. using stepping motor
G05B 19/042 - Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
The invention relates to a method for inactivating viruses in a fluid, in which method: a starting fluid (AF) containing active viruses is mixed with a virus-inactivating reagent (R1) in a first mixing assembly (2) to form a reactive fluid (F); the active viruses are inactivated via incubation of the reactive fluid (F); and the inactivation of the viruses in the reactive fluid (F) is stopped by mixing the reactive fluid (F) with a further reagent (R2) or by removing the virus-inactivating reagent (R1) from the reactive fluid (F), as a result of which a resulting fluid (RF) is produced. According to the invention, the active viruses are inactivated via incubation of the reactive fluid (F) in a container assembly (3) which comprises a plurality of separate containers (4) and differs from the first mixing assembly (2), wherein the reactive fluid (F) is conducted from the first mixing assembly (2) to the container assembly (3) prior to incubation.
The invention relates to a method for generating and purifying viral vectors (V), wherein the viral vectors (V) are generated by cells in a fluid (F) and wherein the fluid (F) comprising the viral vectors (V) is guided through a tangential flow filtration arrangement (2) for purification, whereby the viral vectors (V) are purified in the fluid (F). It is proposed that the cells and cell debris from the cells, which are contained in the fluid (F) comprising the viral vectors (V) after generating the viral vectors (V), are at least partially separated from the viral vectors (V) by a fluidized bed centrifuge (5) before purification by the tangential flow filtration arrangement (2).
C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
The present invention relates to methods for the purification of viruses, virus-like particles, extracellular vesicles, proteins having a diameter of 5 nm or more, protein complexes having a diameter of 5 nm or more, nucleic acids having a diameter of 5 nm or more, protein-nucleic acid complexes having a diameter of 5 nm or more, and/or molecules having a diameter of 5 nm or more, by filtration-supported polyalkylene glycol precipitation, and to filtration modules that can be used in said methods.
Embodiments include a device for continuous virus inactivation during a protein production process comprising a first and a second fluid inlet, a first mixer, and a fluid outlet. A first liquid stream containing a target protein is introducible into the device through the first fluid inlet and is combinable in a predefined volume ratio with a second, virus-inactivating liquid stream introducible into the device through the second fluid inlet to form a third, reactive liquid stream which is conducted through the first mixer for mixing in order to generate predefined, virus-inactivating conditions. The device further comprises a head part and, downstream of the first mixer and upstream of the fluid outlet, a residence time arrangement fluidically connected to the head part for provision of a minimum residence time of the third stream, wherein the head part and the residence time arrangement are rigidly fastened to each other.
Systems and methods for characterizing a plurality of particles suspended in a solution are described. In some embodiments, an ultrasonic interrogation signal may be emitted into a solution including a plurality of particles suspended in the solution. A resulting ultrasonic spectrum may be sensed and provided to a trained statistical model of the solution. The trained statistical model may then determine one or more properties of the plurality of particles.
A method for operating a bioprocess installation with an electronic process control and at least one bioprocess unit, wherein the bioprocess unit comprises a cell broth source with a first receptacle for cell broth including cultivation media and cells, establishing a culture environment for cell cultivation and/or bio production, wherein the bioprocess unit comprises a clarification setup with a centrifuge for the clarification of the cell broth by centrifugation, with a liquid pumping arrangement assigned to the centrifuge and with a liquid network with a number of liquid lines communicating with the liquid pumping arrangement, wherein out of a first culture environment established by the first receptacle, the cell broth is transfered to the centrifuge via the liquid network, which centrifuge is operated in a forward operation for cell separation and/or cell washing and in a backward operation for cell discharging.
The invention relates to a method for operating a continuous downstream process, in particular a continuous virus activation or inline dilution process, using a bio-process arrangement (1), wherein the bio-process arrangement (1) has a compensating container (2) with a first fluid inlet (3), configured for introduction of a concentrate stream, in particular a product or buffer concentrate stream, into the compensating container (2), with at least one second fluid inlet (4), configured for introduction of a diluent stream, in particular a product or buffer diluent stream, into the compensating container (2), with a fluid outlet (5), configured to discharge a liquid stream from the compensating container (2), wherein the bio-process arrangement (1) has a conveying arrangement (6) for fluid conveyance, which is allocated to the first fluid inlet (3) and to the second fluid inlet (4) for conveying in each case at least one liquid stream into the compensating container (2), and is allocated to the fluid outlet (5) for conveying a liquid stream out of the compensating container (2), and wherein the bio-process arrangement (1) has an electronic process control (7). It is proposed that the electronic process control (7) adapts, by controlling the conveying arrangement (6) in an open-loop and/or closed-loop manner, a fill level in the compensating container (2) such that a predefined minimum fill level in the compensating container (2) is always reached.
B01F 23/40 - Mixing liquids with liquidsEmulsifying
B01F 35/83 - Forming a predetermined ratio of the substances to be mixed by controlling the ratio of two or more flows, e.g. using flow sensing or flow controlling devices
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Cartridges for filtering machines; filters for machines. Laboratory filters. Filters for industrial and household use (terms too vague in
the opinion of the International Bureau - Rule 13 (2) (b) of
the Common Regulations). filters for industrial
installations (terms too vague in the opinion of the
International Bureau - Rule 13 (2) (b) of the Common
Regulations).
filter cartridges for water purification;
filters for water filtering apparatus; water purification
filters; water treatment filters; water filters for
industrial purposes; industrial installations for filtering
liquids; water filtering apparatus; water filtering
installations; water filtering units; filters (not being
parts of machines) as components of filtration systems or as
independent filters for the sterilization and purification
of liquids and gases for the electronic, pharmaceutical,
food, luxury consumer goods and cosmetics industries in
general.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Filter housings being parts of machines; filters being parts
of machines; filters for gases [machines]; filters for
machines; membrane filters for use as parts of machines. Filtering units for laboratory use; laboratory filters. Air filters; air filters for industrial use; air filters for
industrial installations; filters for cleaning and
sterilizing air; filters for gases [household or industrial
installations]; filters for cleaning and sterilizing gases
[parts of industrial installations]; filter elements for the
air vents of water supply tanks; filters for industrial and
household use (term considered too vague by the
International Bureau - Rule 13 (2) (b) of the Regulations);
filters for industrial installations (term considered too
vague by the International Bureau - Rule 13 (2) (b) of the
Regulations); filters for refrigeration systems; filters for
use with bioreactors and fermenters; filters for venting
storage tanks; industrial air filter machines; air filter
installations; filters, in particular filter cartridges and
membrane filters for single use as parts of filter systems
or as stand-alone filters for the sterilization and
purification of liquids and gases in the electronics,
pharmaceutical, food, beverage, luxury goods, cosmetics and
general chemical industries.
79.
METHOD FOR PERFORMING AN INTEGRITY TEST ON A TESTING CONSUMABLE OF A BIOPROCESS INSTALLATION
The invention relates to a method for performing an integrity test on a testing consumable (1) of a bioprocess installation using a test arrangement (2), wherein during a test routine (26), which is being performed by a test control unit (3) of the test arrangement (2), a predetermined test procedure is being applied to the testing consumable (1) via the test arrangement (2) and an integrity state is being derived from process data that have been collected during the test routine (26). It is proposed that during the test routine (26), early process data (27), that are being generated by a testing sensor arrangement (4) of the test arrangement (2) in an early stage of the test procedure, are being used to determine the integrity state of the testing consumable (1) by correlating in a correlating step (28) the early process data (27) to a system model (29) of a reference module (30), which reference module (30) emulates a, in particular integral, testing consumable (1) only in view of predetermined process data.
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
B01D 65/10 - Testing of membranes or membrane apparatusDetecting or repairing leaks
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G01M 3/22 - Investigating fluid tightness of structures by using fluid or vacuum by detecting the presence of fluid at the leakage point using special tracer materials, e.g. dye, fluorescent material, radioactive material for pipes, cables, or tubesInvestigating fluid tightness of structures by using fluid or vacuum by detecting the presence of fluid at the leakage point using special tracer materials, e.g. dye, fluorescent material, radioactive material for pipe joints or sealsInvestigating fluid tightness of structures by using fluid or vacuum by detecting the presence of fluid at the leakage point using special tracer materials, e.g. dye, fluorescent material, radioactive material for valves
G01N 15/08 - Investigating permeability, pore volume, or surface area of porous materials
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; Filtration membranes for laboratory use; Laboratory filtration apparatus and filtering materials for the filtration of liquids and gases; Filters, for use in relation to the following goods: Laboratory apparatus in the biopharmaceutical industry; Filtration units, For laboratory use. Filters, For industrial purposes; Filtration units; Filtration units, namely Filter cartridges, Filter capsules, Filter modules, Filter capsules and Disposable filters, For industrial purposes; Filters, namely Small filters, disposable filters, filter cartridges, filter modules and diaphragm filters being parts of filtering units or being independent filters, for sterilisation and purification of liquids and gases in the beverage, food and general chemical industry; Filters, specifically filter cartridges and membrane filters for single use as parts of filter systems or as stand-alone filters for the sterilization and purification of liquids and gases in the electronics, pharmaceutical, food, beverage, luxury food, cosmetics and general chemical industries.
81.
HOLDING DEVICE FOR A CONTAINER, HAVING A MAGNETIC DRIVE FOR A SEPARATE AGITATOR SHAFT
The invention relates to a holding device (26) for a container, in particular a bioreactor, comprising a magnetic drive (30) for a separate agitator shaft (28) and a lever device (10) for releasing the agitator shaft (28) from the magnetic drive (30). The magnetic drive (30) has a first coupling side (32) and the agitator shaft (28) has a second coupling side (34) of a magnetic coupling, said second coupling side being connectable to the first coupling side (32). The lever device (10) has at least one pressure portion (22; 64) and is pivotable between a closing position and a release position. As the lever device (10) is pivoted into the release position, the pressure portion (22; 64) is moved downwards in order to act directly or indirectly on the agitator shaft (28).
B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; Filtration membranes for laboratory use; Filters, for use in relation to the following goods: Laboratory apparatus in the biopharmaceutical industry; Filtration units, For laboratory use; Breather filters, For laboratory use. Filters, For industrial purposes; Filtration units, namely Filter cartridges, Filter capsules and Disposable filters, For industrial purposes; Filters, namely Small filters, disposable filters, filter cartridges, filter modules and diaphragm filters being parts of filtering units or being independent filters, for sterilisation and purification of liquids and gases in the beverage, food and general chemical industry; Filters, specifically filter cartridges and membrane filters for single use as parts of filter systems or as stand-alone filters for the sterilization and purification of liquids and gases in the electronics, pharmaceutical, food, beverage, luxury food, cosmetics and general chemical industries; Air filters; Air filters for industrial use; Air filters for industrial installations; Gas filters (industrial installations); Industrial air filter machines; Air filtering installations; Filters for purifying gaseous media and sterile ventilation of closed containers, conduits and tube systems; Filters for cleaning and sterilizing air; Parts of industrial installations, namely Filters for cleaning and sterilizing gases; Filters, for use in relation to the following goods: Transport container; Filters, for use in relation to the following goods: Containers, of plastic; Container attachment filters; Ventilation filters, For industrial purposes; Filters for the sterile gas exchange of containers; Filter elements for the air vents of water supply tanks; Filters for venting storage tanks; Filters for refrigeration systems; Filters, for use in relation to the following goods: Bio-reactors and Fermenters.
83.
METHOD OF CHANGING CULTURE MEDIUM OF A CULTURE USING SPINFILTERS
The present invention relates to a method of expanding stem cells cultured as cell aggregates in a suspension culture changing culture medium and a method of medium exchange for the same cells characterized in the use of a rotating mesh such as a spinfilter device. The present invention further relates to a use of a rotating mesh for medium exchange in a suspension culture of stem cells.
The invention relates to a method for operating a bioprocess installation (1) for production of a bioproduct, wherein the bioprocess installation (1) comprises a source receptacle (2) for cell cultivation, a harvest receptacle (3) for bioproduction and a clarification setup (4) with a centrifuge (12), wherein the source receptacle (2) is operated in a cyclical production mode comprising the steps of: a) starting the cyclical production mode in the source receptacle (2) b) cultivating the cells in the source receptacle (2), thereby obtaining a cell broth (7) comprising cultivated cells, c) discharging a discharge fraction (16) of the cell broth (7) from the source receptacle (2), d) combining a restart fraction (17) of the cell broth (7) with fresh cultivation medium (8) and repeating step b), e) repeating steps c) and d) at least once and/or f) discharging the cell broth (7) obtained from step d) from the source receptacle (2) stopping the cyclical production mode, obtaining a discharge fraction (16), wherein the method further comprises the steps of: i) centrifuging the discharge fraction (16) via the centrifuge (12), thereby separating the discharge fraction (16) into at least a centrifuged discharge fraction (19) and supernatant (14) and preferably bioproduct, ii) operating the harvest receptacle (3) in a production mode, wherein steps i) and ii) are executed at least twice.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
17 - Rubber and plastic; packing and insulating materials
Goods & Services
Filter modules, namely filter capsules, filter candles, filter cassettes, coil modules, filter coils, filter coils in connection with filter centrifuges, band filters, small filters, small filters in connection with centrifuge tubes and as centrifugal concentrators, disposable filters; membrane filters and filter matting being parts of machines, for use in biotechnology, environmental protection, for the drinks, food, luxury items, pharmaceutical, cosmetics, electronics and general chemical industries, and for use in genetic engineering, medicine and in laboratories, and for treatment and analysis of water and effluent; filters being parts of machines; membrane filters for use as part of machines. Laboratory filters; Filtering units for laboratory use; liquid filter units, namely, filter cartridges, plastic hoses and hose connectors sold as a set for laboratory use; membranes for filtration [scientific]; filters for use with laboratory chromatography apparatus; membrane filters and filter matting with adsorption properties, membrane adsorbers, namely adsorption membranes, ion exchange membranes, ligand membranes and activated membranes in the form of hollow fibre membranes, tube membranes and flat membranes as strips or cut to size for use in apparatus, filters and filter modules for the aforesaid fields of application; membrane filters and filter matting as parts of microtitre plates; membrane filters and filter matting as parts of diagnostic tests such as diagnostic assays and immunoassays; membrane filters and filter matting as parts of filter systems or as independent filters for chromatographic analyses and chemical separation, reconcentration, for sterilisation and ultrapurification of fluids; filters and filter modules for filtering and reconcentration of biological solutions, namely of viral and bacterial suspensions; filters and filter modules for treating blood and other bodily fluids. Filters and filtering units, for industrial use; filters for filtering fluids, filter modules, filter candles and filter capsules; liquid filter units, namely, filter cartridges, plastic hoses and hose connectors, sold as a set, for industrial installations; Filters for water filtering apparatus; Cartridge filtration units [water treatment equipment]; filters for use with industrial chromatography apparatus; apparatus for water treatment and for preparing solutions in the pharmaceutical, medical and laboratory fields; equipment for de-ionising water; equipment for de-pyrogenisation of solutions and for separating harmful substances from fluids; filters and filter modules for desalination of protein solutions and other biological media; filters and filter modules for analysis and separation of ions, macromolecules and biomolecules, namely carbohydrates, peptides, proteins and nucleic acids and for separating heavy metals and harmful substances from fluids; filters for industrial use, namely, membrane filters and filter matting having applications in biotechnology, environmental protection, the drinks, food, luxury items, pharmaceutical, cosmetics, electronics and general chemical industries, and in genetic engineering, medicine and laboratories, and the treatment and analysis of water and effluents. Membranes and semi-processed synthetic filtering materials; Polymeric membranes; Polymeric porous membranes in hollow fibre form; Polymeric porous membranes in sheet form; Transfer membranes of cellulose derivatives; Transfer membranes of cellulose; Transfer membranes of polyamides; Transfer membranes of polyvinylidene fluoride; Cross-linked polymeric membranes in sheet form; Cross-linked polymeric membranes in hollow fibre form.
The invention relates to a method for a dynamic in-line mixing process of a pressurized medium (1), which contains a liquid and at least one additional liquid or solid constituent, in a bioprocess assembly (3). The liquid is combined with the at least one additional liquid or solid constituent in a specified volume ratio at an opening point (4) in order to form a resulting liquid flow (5), wherein the bioprocess assembly (3) has a pump assembly (8) with a first pump (9), the first pump (9) is arranged in the line (7) of the line assembly (6), the first pump (9) is designed as a rotary pump (10), in particular a centrifugal pump, which is designed for a dynamic in-line mixing process of the medium (1), the first pump (9) has a liquid inlet (9a) which forms the suction side of the pump when operated as intended and a liquid outlet (9b) which forms the pressure side of the pump when operated as intended, and the medium (1) is conducted through the rotary pump (10) for a dynamic in-line mixing process. According to the invention, the medium flows through the rotary pump (10) in the opposite flow direction, in comparison to the intended operation, for the dynamic in-line mixing process.
A device assembly for controlling an integrated continuous pharmaceutical or biopharmaceutical manufacturing process including a first process equipment for performing a first process step; a second process equipment for performing a second process step subsequent to the first process step; a single measuring unit for measurement of a set of signals of a liquid process medium at a single location, the measured signals depending on first and second parameters; and an evaluation and control unit for evaluating the measured signals to determine values of the first and second parameters. The evaluation and control unit determines first and second corrective feedback based on the values of the first and second parameters, respectively. The evaluation and control unit controls the first process step by providing the first corrective feedback to the first process equipment and controls the second process step by providing the second corrective feedback to the second process equipment.
Disclosed is inter alia a method for validating a filter unit, wherein the method comprises: - guiding a fluid flow of a fluid, after the fluid flow passed through the filter unit, to a measurement area that is connected to the filter unit, wherein within the measurement area the fluid is exposed to at least one alternating electric field between at least two electrodes; - obtaining at least one electric signal between the at least two electrodes, wherein the at least one electric signal is at least affected by whether one or more cells of a plurality of cells in the fluid pass through the measurement area; and - determining, at least partially based on the obtained at least one electric signal, at least one validity information indicating a validity of the filter unit.
A bioreactor system (10) for carrying out a biological process comprises a container (12) for receiving a liquid biological medium and a gas supply facility for feeding various gases from gas sources (38, 40, 42, 44) into the container (12) in a controlled manner. The gas supply facility has a plurality of gas outlet lines (28, 30, 32) which open into one or more gas supply devices (20) and/or into an overlay gas outlet in the interior of the container (12). Each gas outlet line (28, 30, 32) is connected to multiple gas sources (38, 40, 42, 44) by a respective gas supply line (46, 52, 56, 58) or by a respective branch (48, 50, 54, 60) of a gas supply line (46, 52, 56, 58). A mass flow regulator (62), which is connected to a control unit, is arranged in each gas supply line (46, 52, 56, 58) and in each branch (48, 50, 54, 60). Such a bioreactor system for carrying out a biological process preferably comprises a controller, which comprises a master regulator for a controlled variable, at least one sensor associated with the master regulator and one or more follower regulators with actuators (14, 62), the manipulated variables of which influence the controlled variable in a targeted manner. The controller is designed such that a setpoint value for the controlled variable can be prespecified to the master regulator, the at least one sensor is used for repeated determination of an actual value of the controlled variable, and the master regulator provides to the follower regulators an output signal dependent upon the deviation of the actual value of the controlled variable from the setpoint value of the controlled variable. Control profiles, which are dependent upon the output signal of the master regulator, are assigned to the follower regulators. According to a first alternative, the control profiles contain setpoint values for the actuators (14, 62) of the follower regulators, and the controller is designed such that the setpoint values for the actuators (14, 62) can be input in the physical unit of their manipulated values. According to a second alternative, the control profiles contain setpoint values for a parameter that relates directly to the biological process and can be influenced in a targeted manner by the actuators (14, 62), and the controller is designed such that it automatically assigns setpoint values for the actuators of the follower regulators to the parameter setpoint values.
The invention relates to a flow-through centrifuge (1), which can be used for example for biotechnological applications, in particular in the form of a blood centrifuge (2). A drive and/or transmission assembly (19) of the through-flow centrifuge (1) has a planetary transmission (62). At least one planetary belt pulley (34; 36) is rotatably mounted on a rotating planet carrier (18) in the planetary transmission (62), and the torque of the planetary belt pulley (34; 36) is transmitted via a belt (33; 37), wherein the planet carrier (18) and the belt pulley (34; 36) are driven at different rotational speeds. According to the invention, the planet carrier (18) is held via a belt-tensioning unit (17) which rotates together with the planet carrier (18), and the distance (42) between the planet carrier (18) and a rotor axis (4) can be modified via the belt-tensioning unit (17) in order to set the tension of the belt (33; 37).
A method for mounting at least one component on an installation includes attaching a carrier directly or indirectly to the installation, where the installation has at least one frame with at least two arms, at least one base plate, and at least one deformable element. The method further includes displacing the component toward the deformable element such that the deformable element is displaced from a receiving state (AUZ), in which the deformable element is ready to receive the component of the installation, toward a locked state (ARZ), as a result of which the deformable element surrounds and mounts the component of the installation at least in part.
A method for providing an integrity test of a double filter capsule can include providing the double filter capsule in such a way that in a filtration process the medium to be filtered flows in its flow direction from an upstream chamber in the housing through a first filter into an intermediate chamber between the first filter and a second filter and then through the second filter to an outlet. The housing can have an upstream-chamber access point for feeding a test fluid into the upstream chamber and an intermediate-chamber access point for feeding the test fluid into the intermediate chamber. Additional steps can include providing a connecting line between the upstream-chamber access point and the intermediate-chamber access point and determining the state of integrity of the double filter capsule on the basis of at least two test phases.
B01D 65/10 - Testing of membranes or membrane apparatusDetecting or repairing leaks
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
B01D 29/56 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
93.
CHROMATOGRAPHIC MATERIAL AND METHOD OF PRODUCING SAME
The present invention relates to a chromatographic material comprising a polymer network material-based self-supporting bi-continuous separation matrix for adsorptive material separation in liquid media, and a method of producing the chromatographic material comprising a polymer network material-based self-supporting bi-continuous separation matrix.
B01J 20/24 - Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
B01J 20/30 - Processes for preparing, regenerating or reactivating
B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
The invention is directed to a method for producing a roll (1) or sheet of membrane units (2) for a membrane product (3) such as a lateral flow test from a roll (6) or sheet of membrane material (7), by means of a primary production arrangement (9), wherein the roll (6) or sheet of membrane material (7) is processed into a roll (1) or sheet of membrane units (2) in a primary processing routine, wherein in the primary processing routine, for generating the membrane units (2), a fluidic structure (11), in particular a hydrophobic structure, for defining fluid flow through the membrane material (7) is introduced into the membrane material (7) by means of a processing tool (12), wherein an evaluation routine is performed by means of an evaluation arrangement (15) comprising a sensor arrangement (16) and an evaluation control (17). It is proposed that in the evaluation routine, evaluation images (18) of the fluidic structures (11) of the membrane units (2) are generated by means of the sensor arrangement (16) and evaluation data (19) are generated by means of the evaluation control (17) and that the evaluation data (19) represent the deviation in predefined geometrical properties of the fluidic structure (11) in the respective evaluation image (18) with respect to the fluidic structure (11) in a reference image (21).
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 33/531 - Production of immunochemical test materials
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
95.
LIPID NANOPARTICLE PRODUCTION SYSTEM AND METHOD OF MONITORING AND CONTROLLING THE SAME
The present invention relates to automated nanoparticle synthesis systems and computer-implemented methods of monitoring and controlling a process of manufacturing lipid nanoparticles (LNPs) containing nucleic acid cargo. The present invention furthermore relates to a computer program product comprising computer-readable instructions, which, when loaded and executed on a computer system, causes the computer system to perform operations according to said methods.
A method for ensuring a microbiological purity of a single-use device for carrying out a biotechnological process, the single-use device including at least one gamma-sterilizable component which is formed from materials suitable for a sterilization by gamma radiation, and at least one non-gamma-sterilizable subunit which contains a material unsuitable for a sterilization by gamma radiation. Both the component and the subunit each have an area that comes into contact with a process medium when the biotechnological process is carried out. The method includes the steps of sterilizing the gamma-sterilizable component by gamma radiation; protecting the medium-contacting area of the gamma-sterilizable component by a sterile barrier; sterilizing the non-gamma-sterilizable subunit with superheated steam; protecting the medium-contacting area of the non-gamma-sterilizable subunit by a sterile barrier; removing the sterile barriers; and mounting the gamma-sterilized component and the superheated steam-sterilized subunit in the single-use device immediately after removing the sterile barriers.
The invention relates to a centrifuge (19), in particular a continuous-flow centrifuge, a biotechnical centrifuge or a blood centrifuge. The centrifuge (19) is provided with a coolant circuit (1). According to the invention, the outlet side of a controllable compressor (4) is connected to an inlet side of an evaporator (2) via a bypass line (5) with a valve (20) arranged therein. In a normal operating mode, when the valve (20) is closed, the cooling of a centrifuge bowl (3) occurs exclusively via the control of the compressor (4) and an expansion unit (6). However, if the target temperature in the centrifuge bowl (3) falls below a tolerance range, the valve (20) is opened in order to bring about a heating process and thereby a returning of the temperature in the centrifuge bowl (3) to within the tolerance range.
The present invention relates to tangential flow filtration/chromatography systems, the use thereof, and methods of separation, particularly simultaneously separating one or more target molecules from cells, cell debris and further contaminants contained in e.g. fermentation broth or any other particulate and impurity containing, non-clarified liquid.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
The present invention relates to a filter module comprising an ester-based membrane and at least one boundary member, wherein the peripheral region of the membrane is connected to the at least one boundary member, and wherein the surface of the membrane is saponified in the regions other than the peripheral region connected to the at least one boundary member. Further, the present invention relates to a method of producing such filter module and to the use of such filter module.
The application relates to a sensor holding device for holding a sensor device, in particular a clamp-on flow measurement sensor, the sensor holding device comprising: a holding body configured for holding the sensor device, wherein the holding body comprises: a sensor holding device-side engaging means for engaging with a sensor device-side engaging means of the sensor device when the sensor device is held by the holding body; and a securing means connected to the holding body for securing the sensor device at the holding body, the securing means being moveable relative to the holding body to a first position and to a second position, wherein the securing means moved into the first position allows placing the sensor device onto the holding body and removing the sensor device from the holding body, and wherein the securing means moved into the second position and the sensor holding device-side engaging means engaging the sensor device-side engaging means restrict relative movement of the sensor device along three directions with respect to the holding body such that the sensor device is secured at the holding body.
