The invention relates to a method of transferring a microbial bioprocess from a multi-use bioreactor (1) to a single-use bioreactor (2), wherein the source bioprocess is a batch or fed-batch microbial bioprocess and the source bioprocess arrangement (3) comprises a source bioreactor (5) with a stirrer and the source bioreactor (5) is the multi-use bioreactor (1) which has at least 1000 L production volume of a microbial culture producing a maximum heat of at least 25,000 W and/or the stirrer of the source bioreactor (5) is driven with a maximum torque of at least 120 Nm, wherein the target bioprocess is a continuous microbial bioprocess and the target bioprocess arrangement (4) comprises a target bioreactor (6) and the target bioreactor (6) is the single-use bioreactor (2) which has a production volume of a microbial culture of at most 50 % of the production volume of the source bioreactor (5) and of at most 800 L, the microbial culture of the target bioreactor (6) produces a maximum heat of at most 20,000 W during the target bioprocess and/or the target bioreactor (6) comprises a stirrer driven with a maximum torque of at most 100 Nm, wherein an average amount of the bioproduct produced per time of performance of the target bioprocess by the target bioreactor (6) is at least 90 %, preferably at least 100 %, of an average amount of the bioproduct produced per time of performance of the source bioprocess by the source bioreactor (5).
Described and illustrated is a air vent valve (1) for the use in a (bio)pharmaceutical process, comprising: a first housing (2), a first interior chamber (5) arranged within the first housing, a first inlet (3) for conveying air and/or liquid medium to the first interior chamber (5), a first outlet (4) for conveying air away from the first interior chamber (5), and a first floating member (6) for sealing the first outlet (4), wherein the first inlet (3) and the first outlet (3) are respectively connected via a fluidic connection with the first interior chamber (5), and wherein the first floating member (6) is arranged movable within the first interior chamber (5). To improve air vent valves, preferably to provide a less complex air vent valve, the describe air vent valve it is proposed.
Depicted and described is a Filter module (1) for the tangential flow filtration of a feed medium, comprising: a, preferably cassette-shaped, housing (2), one or more hollow fibers (8) for separating a feed medium into a retentate and a permeate, wherein the housing (2) has one or more transverse extension axes (TEA) and/or one or more longitudinal extension axes (LEA), wherein one or more conduits (9) are arranged in the housing (2), wherein the one or more hollow fibers (8) are arranged in the one or more conduits (9). In order to provide a filter module (1) which allows for the use of hollow fibers (8) with a set, preferably standardized, length while allowing for an individual arrangement of inlets and outlets of the filter module (1) it is proposed that the one or more hollow fibers (8) are at least in sections bent around the one or more transverse extension axes (TEA) and/or the one or more hollow fibers (8) are at least in sections bent around the one or more longitudinal extension axes (LEA).
4.
MECHANICALLY STABLE ULTRAFILTRATION MEMBRANE, AND METHOD FOR PRODUCING SAME
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
A filtration device (10) for filtering a medium, in particular a small-scale filtration device for the production and/or processing of biopharmaceuticals, comprises: a top cover (12) having an unfiltrate inlet (14) and a bottom cover (16) having a filtrate outlet (18), and a plurality of filter supports (28), arranged as a stack between the top cover (12) and the bottom cover (16) along a vertical axis. Each filter support (28) is provided with a flat filter (24), especially a membrane. Each flat filter (24) is sealed to the filter support (28) with a filtrate side facing the respective filter support (28) and an unfiltrate side facing away from the respective filter support (28). The filtration device further comprises one or more intermediate elements (30), preferably one or more intermediate plates. A first intermediate element (30) is arranged between the stacked filter supports (28), dividing the filter supports (28) into an upper group (32) above the intermediate element (30) and a lower group (34) below the intermediate element (30). The first distribution flow paths lead from the unfiltrate inlet (14) to all unfiltrate sides of the flat filters (24) of the upper group (32). The first intermediate element (30) has a structure that blocks the first distribution flow paths from reaching the unfiltrate sides of the flat filters (24) of the lower group (34) and provides first collecting paths for collecting the medium that has passed through the flat filters (24) of the upper group (32). Second distribution flow paths lead from the first collecting paths to all unfiltrate sides of the flat filter(s) (24) of the lower group (34). Second collecting paths collect the medium that has passed through the flat filter(s) (24) of the lower group (34).
B01D 29/05 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with flat filtering elements supported
B01D 29/41 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with hollow discs side by side on, or around, one or more tubes, e.g. of the leaf type mounted transversely on the tube
B01D 29/56 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
B01D 29/58 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection arranged concentrically or coaxially
The invention is directed to a method for producing a roll (1) or sheet of membrane units (2) for a membrane product (3) such as a lateral flow test from a roll (6) or sheet of membrane material (7), by means of a primary production arrangement (9), wherein the roll (6) or sheet of membrane material (7) is processed into a roll (1) or sheet of membrane units (2) in a primary processing routine, wherein in the primary processing routine, for generating the membrane units (2), a fluidic structure (11), in particular a hydrophobic structure, for defining fluid flow through the membrane material (7) is introduced into the membrane material (7) by means of a processing tool (12), wherein an evaluation routine is performed by means of an evaluation arrangement (15) comprising a sensor arrangement (16) and an evaluation control (17). It is proposed that in the evaluation routine, evaluation images (18) of the fluidic structures (11) of the membrane units (2) are generated by means of the sensor arrangement (16) and evaluation data (19) are generated by means of the evaluation control (17) and that the evaluation data (19) represent the deviation in predefined geometrical properties of the fluidic structure (11) in the respective evaluation image (18) with respect to the fluidic structure (11) in a reference image (21).
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; Filtration membranes for laboratory use; Laboratory filtration apparatus and filtering materials for the filtration of liquids and gases; Filters, for use in relation to the following goods: Laboratory apparatus in the biopharmaceutical industry; Filtration units, For laboratory use. Filters, For industrial purposes; Filtration units; Filtration units, namely Filter cartridges, Filter capsules, Filter modules, Filter capsules and Disposable filters, For industrial purposes; Filters, namely Small filters, disposable filters, filter cartridges, filter modules and diaphragm filters being parts of filtering units or being independent filters, for sterilisation and purification of liquids and gases in the beverage, food and general chemical industry; Filters, in particular filter cartridges and membrane filters for single use as parts of filter systems or as stand-alone filters for the sterilization and purification of liquids and gases in the electronics, pharmaceutical, food, beverage, luxury food, cosmetics and general chemical industries.
8.
MEASURING CELL UNIT FOR BIOPHARMA PROCESS SYSTEM, BIOPHARMA PROCESS SYSTEM, AND METHOD FOR EXCHANGING FLUIDS IN BIOPHARMA PROCESS SYSTEM
A measuring cell unit (16) for a bioprocess system is shown, in particular for a biopharma process, comprising a flow pipe unit (28) with a first connection end (30) having an inlet channel (34), a second connection end (32) having an outlet channel (36) and a measuring chamber (38) between the inlet and the outlet channels (34, 36). The first and second connection ends (30, 32) are configured to connect the measuring cell unit (16) into the bioprocess system. Together, the inlet channel (34), the measuring chamber (38) and the outlet channel (36) define portions of a flow duct (40) through the measuring cell unit (16). Further, the flow pipe unit (16) comprises a sideward sensor insert hole (42) opening into the measuring chamber (38) such that a measuring sensor (26) can be inserted. The flow duct (40) further comprises a transitional portion (44) along which a cross-sectional area of the flow duct (40) increases from the inlet channel (34) towards the measuring chamber (38). Additionally, the measuring cell unit (16) comprises at least one flow deflection arm (46) extending into the transitional portion (44) and deflecting a fluid flowing through the flow duct (40). Further, a bioprocess system and a method for displacing a first fluid by a second fluid are shown.
Method for operating a bioprocess installation for production of a bioproduct, wherein the bioprocess installation comprises a source receptacle for cell cultivation, a harvest receptacle for bioproduction and a clarification setup with a centrifuge wherein the source receptacle is operated in a cyclical production mode comprising the steps of: a) starting the cyclical production mode, b) cultivating the cells thereby obtaining a cell broth comprising cultivated cells, c) discharging a discharge fraction of the cell broth, d) combining a restart fraction of the cell broth with fresh cultivation medium and repeating step b), e) repeating steps c) and d) at least once and/or f) discharging the cell broth obtained from step d), obtaining a discharge fraction, wherein the method further comprises the steps: i) centrifuging the discharge fraction via the centrifuge, ii) operating the harvest receptacle in a production mode, wherein steps i) and ii) are executed at least twice.
A method for the dynamic inline mixing of a pressurized medium containing a liquid and at least one further liquid or solid constituent in a bioprocess arrangement, the liquid being merged with the at least one further liquid or solid constituent in a predefined volume ratio at an opening point to form a resulting liquid flow, the bioprocess arrangement having a pump arrangement with a first pump, the first pump being arranged in the line of the line arrangement, the first pump being designed as a rotary pump configured for the dynamic inline mixing of the medium, the first pump having a liquid inlet, which during intended operation forms the suction side of the pump, and a liquid outlet, which during intended operation forms the pressure side of the pump, and the medium being conducted through the rotary pump for the purpose of dynamic inline mixing.
B01F 25/00 - Flow mixersMixers for falling materials, e.g. solid particles
B01F 35/213 - Measuring of the properties of the mixtures, e.g. temperature, density or colour
B01F 35/83 - Forming a predetermined ratio of the substances to be mixed by controlling the ratio of two or more flows, e.g. using flow sensing or flow controlling devices
The invention relates to a method for adjusting the pH of a process liquid in a continuous process, comprising (a.) providing the process liquid in a first volume flow having a first flow rate, (b.) providing at least one treatment liquid in a second volume flow having a second flow rate, (c.) mixing the at least one treatment liquid with the process liquid to produce a pH-adjusted process liquid, wherein the second flow rate is composed of a predetermined portion and a variable portion, and wherein the variable portion is controlled based on at least one liquid parameter of the pH-adjusted process liquid. The method is characterized in that the predetermined portion is determined based on the pH value and/or the UV absorbance and/or the conductivity of the process liquid.
The present invention relates to a flow system for the continuous treatment of a process liquid. The flow system comprises a recirculation loop comprising a compensation tank having an inlet and an outlet, a circulation line connecting the inlet and outlet of the compensation tank to one another, at least one sensor for determining at least one liquid parameter, supply lines for the process liquid and at least one treatment liquid which are connected to the recirculation loop, and discharge lines for treated process liquid and waste streams connected to the recirculation loop.
The present invention relates to a filter module comprising an ester-based membrane and at least one boundary member, wherein the peripheral region of the membrane is connected to the at least one boundary member, and wherein the surface of the membrane is saponified in the regions other than the peripheral region connected to the at least one boundary member. Further, the present invention relates to a method of producing such filter module and to the use of such filter module.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; membrane filters for laboratory use; filters for scientific and research purposes; filtration apparatus for laboratory use; filter cartridges for laboratory filtration; membrane filtration units for laboratory applications; parts and fittings for the aforesaid goods. Filters and filtering apparatus for industrial use; membrane filters for the filtration of liquids; pre-filtration units for industrial liquid processing; filters for the food and beverage industry; industrial filtration cartridges; liquid purification filters for industrial purposes; parts and fittings for the aforesaid goods.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Filters for scientific and laboratory use; filter cartridges for scientific and laboratory use; chromatography cartridges and chromatography modules for biochemical and biological separations; membrane chromatography modules for scientific purposes; ion exchange chromatography modules for laboratory use; separation and purification apparatus for laboratory and scientific use; chromatography devices and consumables for laboratory use; parts and fittings for the aforesaid goods. Apparatus for purification and separation of liquids for industrial use; industrial filtration and chromatography apparatus; installations for purification of biological and chemical substances; industrial separation apparatus for downstream processing; parts and fittings for the aforesaid goods.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; laboratory filter modules; membrane filters for laboratory use; filters for scientific and research purposes; filtration apparatus and filtration modules for laboratory use; microfiltration and clarification filters for laboratory applications; parts and fittings for the aforesaid goods. Filters and filtering apparatus for industrial use; industrial filter modules; clarification and microbe-retentive filters for liquid processing; filter modules for the food and beverage industry; filtration modules and filters for the purification and clarification of liquids; parts and fittings for the aforesaid goods.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Filters for scientific and laboratory use; filter cartridges for scientific and laboratory use; chromatography cartridges and chromatography modules for biochemical and biological separations; membrane chromatography modules for scientific purposes; ion exchange chromatography modules for laboratory use; separation and purification apparatus for laboratory and scientific use; chromatography devices and consumables for laboratory use; parts and fittings for the aforesaid goods. Apparatus for purification and separation of liquids for industrial use; industrial filtration and chromatography apparatus; installations for purification of biological and chemical substances; industrial separation apparatus for downstream processing; parts and fittings for the aforesaid goods.
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Filters for scientific and laboratory use; filter cartridges for scientific and laboratory use; chromatography cartridges and chromatography modules for biochemical and biological separations; membrane chromatography modules for scientific purposes; ion exchange chromatography modules for laboratory use; separation and purification apparatus for laboratory and scientific use; chromatography devices and consumables for laboratory use; parts and fittings for the aforesaid goods. Apparatus for purification and separation of liquids for industrial use; industrial filtration and chromatography apparatus; installations for purification of biological and chemical substances; industrial separation apparatus for downstream processing; parts and fittings for the aforesaid goods.
A bioreactor system for carrying out a biological process including a vessel for receiving a liquid biological medium, and a gassing installation for controlled supply of different gases from gas sources into the vessel. The gassing installation has a plurality of gas outlet lines which open into gassing devices and/or into an overlay gas outlet in the interior of the vessel. Each gas outlet line is connected to a plurality of gas sources via a respective gas supply line or via a respective branch from a gas supply line. A mass flow controller connected to a controller is arranged in each gas supply line and in each branch. The system may include a controller with a master controller for a controlled variable, at least one sensor associated with the master controller, and one or more slave controllers having actuators, the manipulated variables of which purposefully influence the controlled variable.
A bioprocess system for a bioprocess operation with a display arrangement, wherein the bioprocess system comprises system components comprising at least one monitored system component, wherein the system components can be assembled into an assembled state in which the bioprocess system is operational for the bioprocess operation and can be disassembled into a disassembled state in which the bioprocess system is not operational for the bioprocess operation, The bioprocess system comprises a control arrangement and the display arrangement comprises at least one display component. In an assembled state of the bioprocess system during a bioprocess operation the control arrangement displays process information via the display arrangement and/or wherein during assembly of the bioprocess system the control arrangement displays assembly information via the display arrangement, wherein the process information and/or the assembly information concern at least the monitored system component and the display arrangement comprises at least one display component.
The present invention relates to a method for validating the ability of a test filter unit to produce a sterile effluent. The method according to the present disclosure has the advantage that it allows simulating cumulative stress incurred by the filter during use with consideration to time and pressure, as closely as is feasible. A further advantage of the method according to the present disclosure is that it allows evaluating the impact of the stress incurred by the process filter during PUPSIT, while factoring in lab scale equipment/limitations and differing objectives, e.g., performing PUPSIT during manufacturing vs. filter validation.
The present invention relates to a method for validating the ability of a test filter unit to produce a sterile effluent. The method according to the present disclosure has the advantage that it allows simulating cumulative stress incurred by the filter during use with consideration to time and pressure, as closely as is feasible. A further advantage of the method according to the present disclosure is that it allows evaluating the impact of the stress incurred by the process filter during PUPSIT, while factoring in lab scale equipment/limitations and differing objectives, e.g., performing PUPSIT during manufacturing vs. filter validation.
A method of determining a flow velocity of a liquid medium comprises the following steps: a) measuring at least one parameter of the medium that is correlated with the density of the medium; b) predicting the density based on the measured parameter; c) measuring a flow velocity of the medium using an ultrasonic flowmeter (10); and d) calculating a corrected flow velocity based on the measured flow velocity and the predicted density. A device assembly for determining a flow velocity of a liquid medium comprises an ultrasonic flowmeter (10) for measuring a flow velocity of the medium, and at least one sensor (24) for measuring a parameter of the medium that is correlated with the density of the medium. The device assembly further comprises a processing unit (26) for predicting the density based on the measured parameter, and for calculating a corrected flow velocity based on the measured flow velocity and the predicted density. The device assembly is configured to perform the method defined above.
G01F 15/02 - Compensating or correcting for variations in pressure, density, or temperature
G01N 9/24 - Investigating density or specific gravity of materialsAnalysing materials by determining density or specific gravity by observing the transmission of wave or particle radiation through the material
G01F 1/667 - Arrangements of transducers for ultrasonic flowmetersCircuits for operating ultrasonic flowmeters
25.
TUBE REACTOR MODULE, USE OF TUBE REACTOR MODULE, AND INCUBATION DEVICE
The application relates to a tube reactor module for controlling a reaction time of a process fluid in dynamic operation, in particular during viral inactivation in the process fluid, comprising: an inlet for receiving the process fluid; an outlet for discharging the process fluid; a tubular flow path comprising a serpentine pattern for guiding the process fluid from the inlet to the outlet, the tubular flow path comprising a sequence of fluidly connected alternatingly bent curve sections, wherein each curve section is shaped such that a flow direction of the process fluid changes by at least approximately 90°, in particular by between approximately 135° and 225°, and wherein the tubular flow path comprises a first cross-section perpendicular to the flow direction of the process fluid at respective apexes of the curve sections and a second cross-section perpendicular to the flow direction of the process fluid at respective end portions of the curve sections, wherein the first cross-section is different from the second cross-section.
The invention relates to a method of separating empty viral capsids and full viral capsids, the method comprising: providing a viral capsid preparation comprising the empty viral capsids and the full viral capsids, wherein the viral capsid preparation comprises a first salt concentration; contacting the viral capsid preparation with a stationary phase surface allowing binding of the full viral capsids to the stationary phase surface, wherein the empty viral capsids at least partially do not bind to the stationary phase surface; and conducting a one-step and/or linear elution in which the full capsids are eluted by contacting the stationary phase obtained in step (ii) with an elution solution comprising a second salt concentration, wherein the first salt concentration is lower than the second salt concentration.
B01D 15/12 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
G01N 30/96 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography using ion-exchange
A sensor holding device holds a sensor device and includes a holding body configured for holding the sensor device and the holding body includes a sensor holding device-side engaging means for engaging with a sensor device-side engaging means of the sensor device when the sensor device is held by the holding body, and a securing means connected to the holding body for securing the sensor device at the holding body that is moveable relative to the holding body to a first position and to a second position.
Bioreactor, a Bioreactor Vessel and a Closure Component for a Bioreactor Vessel A closure component is provided for closing an upper opening of a rigid-walled bioreactor vessel, in use the vessel containing a biological medium and the upper opening providing access to the interior of the vessel. The component has: a lid having a mounting for fixing to a rim of the upper opening such that the lid closes the upper opening; a shaft which extends downwards from the lid; and one or more impellers mounted to the shaft. The mounting forms a bearing with the rim of the opening when fixed thereto. The lid, the shaft and the one or more impellers are integrally formed such that the component is a one-piece assembly in which the lid, the shaft and the one or more impellers are rotatable together as a single unit to stir the contents of the vessel.
Bioreactor and a Bioreactor Vessel A rigid-walled bioreactor vessel is provided for containing a biological medium. The vessel includes one or more heat exchange channels for exchanging heat with contents of the vessel. The or each channel is integrated into the wall of the vessel such that the material of the wall also forms the one or more 5 channels. The or each channel has a respective inlet configured to receive a flow of a heat exchange fluid into the channel, and a respective outlet configured to discharge the flow of the heat exchange fluid from the channel
The present invention relates to a filter system, which can be used as a clarification filter/depth filter for cell culture clarification before chromatography and/or ultrafiltration for protein purification, as well as to a method of separating cells and other contaminants from a fluid containing one or more target components by employing the filter system of the present invention.
B01D 39/16 - Other self-supporting filtering material of organic material, e.g. synthetic fibres
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
The present invention relates to a filter module comprising a membrane (3), an edge structure (2), and an anchoring element (1), wherein the edge structure (2) is arranged over a surface of the membrane (3) in an edge region thereof and is embedded in the anchoring element (1), a difference between melting temperatures of the membrane (3) and the anchoring element (1) is from –25 K to 60 K, and a difference between melting temperatures of the edge structure (2) and the membrane (3) is 15 K or more. Moreover, the present invention relates to a method for producing the filter module as well as the use of the filter module for filtering a fluid medium.
The invention relates to a centrifuge for continuous flow centrifugation, wherein the centrifuge (1) comprises a drum (3), a rotor (4) and a motor (5) for driving the drum (3) and the rotor (4), wherein the centrifuge (1) comprises a drive bearing arrangement (7) with at least two drive roller bearings (8) with an inner race (9), an outer race (10) and a lubricant, wherein one of the drive roller bearings (8) comprises a sealing (11), wherein the drive roller bearings (8) rotate around the common rotation axis (A), wherein the drive bearing arrangement (7) comprises a bearing housing (12) with an outer wall (13). It is proposed that the outer wall (13) comprises a ridge (14) connected to the outer wall (13) by a connection providing a sealing (11) function and extending from the outer wall (13) towards the inner races (9) along the sealing (11).
The invention relates to a centrifuge for continuous flow centrifugation with drum (3), a rotor (4) and a motor (5), wherein the drum (3), driven by the motor (5), rotates around a common rotation axis (A) with a rotational frequency during use of the centrifuge (1), wherein the rotor (4) is coupled to the drum (3), wherein the centrifuge (1) comprises a drive bearing arrangement (7) with at least two drive ball bearings (8), wherein the drive ball bearings (8) each comprise an inner race (9), an outer race (10) and a bearing axis (B), wherein the drive ball bearings (8) rotate around the common rotation axis (A). It is proposed that the drive ball bearings (8) are angular drive ball bearings (8).
The invention relates to a pinch valve (2), including a pinching member (10), a base member (20) with a support section (22) facing the pinching member (10) and configured for receiving a tube (T), wherein the pinching member (10) is movable relative to the base member (20) along a pinching direction (4) for pinching the tube (T), and the support section (22) and/or the pinching member (10) comprises a recess (26) extending at least along the pinching direction (4) for receiving the tube (T) sectionally.
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
A61M 39/28 - Clamping means for squeezing flexible tubes, e.g. roller clamps
The invention relates to a centrifuge for continuous flow centrifugation with a drum (3), a rotor (4) and a motor (5) for driving the drum (3) and the rotor (4), wherein the drum (3), driven by the motor (5), rotates around a common rotation axis (A) with a rotational frequency during use of the centrifuge (1), wherein the rotor (4) is coupled to the drum (3), wherein the rotor (4) rotates around the common rotation axis (A) with the double of the rotational frequency, wherein the centrifuge (1) comprises at least one main bearing (7) between the rotor (4) and the drum (3) via which the rotor (4) is mounted onto the drum (3). It is proposed that the rotor (4) comprises at least one cooling fin (10) which moves air in a direction radially outwards from the common rotation axis (A) when the rotor (4) rotates.
The present invention relates to a filtration system for sterile filtration of particles having a hydrodynamic diameter of at most 400 nm as well as a method for sterile filtration of particles having a hydrodynamic diameter of at most 400 nm, both of which achieve a high yield of target molecules and sterility.
The present invention relates to a method of purifying biomolecules having a hydrodynamic diameter of at least 20 nm by using an integral porous polysaccharide membrane having chromatographically active centers. Moreover, the present invention relates to said integral porous polysaccharide membrane which has chromatographically active centers capable of binding a biomolecule having a hydrodynamic diameter of at least 20 nm, as well as a use of said membrane for purifying at least one type of biomolecules having a hydrodynamic diameter of at least 20 nm.
B01D 15/32 - Bonded phase chromatography, e.g. with normal bonded phase, reversed phase or hydrophobic interaction
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
B01D 71/82 - Macromolecular material not specifically provided for in a single one of groups characterised by the presence of specified groups, e.g. introduced by chemical after-treatment
38.
MODULAR SYSTEM FOR PROVIDING A BIOPROCESS DEVICE ASSEMBLY
A modular system for providing a bioprocess device assembly including a rigid skid and grid-modules. The skid includes identical plug-in structures arranged in a regular two-dimensional grid. The grid arrangement defines two-dimensional plug-in fields. At least some of the grid-modules have a matching counterpart plug-in structure. At least some of the grid-modules, in an installed state, have a two-dimensional extension in the plane of the grid that is not greater than a standard size of the plug-in fields. At least some of the grid-modules include a connection port to receive a rigid universal flow connector of standard shape and size in a standard position and orientation. At least some of the grid-modules have an integrated fluid line through which a medium to be processed or analyzed can flow. The integrated fluid line is in flow communication with the connection port of the grid-module. The grid-modules includes a flow control grid-module.
A device assembly for calibrating a single-use sensor before, during or after a biopharmaceutical manufacturing process step including a single-use process equipment assembly for performing at least a part of the process step. The single-use process equipment assembly includes a flow line through which a medium flows in a defined direction during the process step and an integrated single-use sensor for measuring or detecting a property at a measurement location in the flow line. The device assembly includes a calibration line and a switch inserted into the flow line upstream of the measurement location. The switch includes a flow line inlet, a flow line outlet, and a calibration line inlet connected to the calibration line. The switch selectively switches between a first (main) flow path and a second (calibration) flow path. The device assembly includes a dedicated reference sensor, reference source, or reference standard solution in the calibration line.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 1/14 - Suction devices, e.g. pumpsEjector devices
A valve device (10), especially for use with a small volume filtration device or for use in a fluid line in a pharmaceutical or biotechnology application, comprises a housing component (12) including a fluid chamber (22) and a fluid transfer opening (14) for establishing fluid communication with a first external fluid component. The valve device (10) further comprises a valve body (32) received in the housing component (12). The valve body (32) includes a hollow section (34), a first end cooperating with the fluid transfer opening (14) inside the housing component (12), a second end for connecting the valve device (10) to a second external fluid component, at least one first valve body opening (38) connecting the hollow section (34) with the fluid chamber (22) of the housing component (12), a second valve body opening (42) for fluid transfer from or to the second external fluid component, and a first thread structure formed at an outer wall of the valve body (32). The valve body (32) is translationally movable along an axis in the housing component (12) between an open position and a closed position. In the closed position of the valve body (32) the second end closes the fluid transfer opening (14) of the housing component (12). In the open position of the valve body (32) the second end is removed from the fluid transfer opening (14) of the housing component (12) so that a fluid connection is established between the fluid transfer opening (14) and the fluid chamber (22). The valve device (10) further comprises an actuator ring element (56) for actuating the valve device (10). The actuator ring element (56) is rotatably mounted on the housing component (12) and includes an accessible structure that can be engaged manually or by a tool to rotate the actuator ring element (56), and a second thread structure formed at an inner wall of the actuator ring element (56). The second thread structure engages the first thread structure of the valve body (32), so that a rotation of the actuator ring element (56) relative to the housing component (12) and the valve body (32) causes the valve body (32) to translationally move in the housing component (12). The valve device (10) further comprises means for sealing the fluid chamber (22) from the outside of the valve device (10).
F16K 3/24 - Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing with sealing faces shaped as surfaces of solids of revolution with cylindrical valve members
F16K 3/26 - Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing with sealing faces shaped as surfaces of solids of revolution with cylindrical valve members with fluid passages in the valve member
F16K 27/04 - Construction of housingsUse of materials therefor of sliding valves
41.
SINGLE-USE FLOW CELL, DETECTION DEVICE, ELECTRONICS DEVICE AND SYSTEM FOR THE USE IN A MULTIANGLE LIGHT SCATTERING MEASUREMENT
The present invention relates to a single-use flow cell (1) for the use in a multiangle light scattering measurement, comprising: a flow cell body (2), and a flow path (3) for passing a sample through the single-use flow cell (1), wherein the flow path (3) is provided at least in sections in the flow cell body (2). In order to provide a single-use flow cell (1) for the use in a multiangle light scattering measurement which can be easily produced it is provided that the single-use flow cell (1) is manufactured at least in sections by cold casting, injection molding, machining and/or additive manufacturing of an optical transparent material. The present invention also relates to a detection device (17), an electronics device (19) and a system (15) for the use in a multiangle light scattering measurement.
The invention relates to a single-use closed bioreactor for culturing, fermenting or processing a biomass with a rigid housing (3) with an interior space (4) with an inner height, an inner width and a volume smaller 100 L, wherein the rigid housing (3) comprises a lid (5) and a body, wherein the body comprises a wall (6), a top and a closed bottom (7) opposite to the top, wherein the top of the body is irreversibly connected to the lid (5) such that the top is closed by the lid (5), wherein the body comprises a bottom through port (11), wherein the bioreactor (1) comprises a stirrer shaft (10), wherein the stirrer shaft (10) is arranged on the lid (5) of the bioreactor (1) and extends from the lid (5) into the interior space (4) of the rigid housing (3), wherein the stirrer shaft (10) comprises a magnetic component, which is configured to interact with a magnetic drive located outside the body, wherein the bioreactor (1) comprises a first rigid support structure (13) extending from the lid (5) into the interior space (4) of the rigid housing (3), wherein the first rigid support structure (13) comprises a, in particular rigid, first conduit (14), wherein the lid (5) comprises a first inlet port (15) connected to the first conduit (14) via which a fluid can be exchanged with the interior space (4) of the rigid housing (3) through the first conduit (14).
The invention relates to a single-use closed bioreactor for culturing, fermenting or processing a biomass with a rigid housing (3) with an interior space (4) with an inner height, an inner width and a volume smaller 100 L, wherein the rigid housing (3) comprises a lid (5) with an upper surface (8) and a lower surface (9) and a body, wherein the body comprises a wall (6), a top and a closed bottom (7) opposite to the top, wherein the top of the body is irreversibly connected to the lid (5) such that the top is closed by the lid (5), wherein the lid (5) comprises a cooling structure (40) for cooling an exhaust gas of the bioreactor (1), wherein the cooling structure (40) comprises a cooling channel (41) with a channel ceiling (42) and a channel floor (43), wherein the cooling channel (41) has a channel inlet (44) at the lower surface (9) of the lid (5) within the interior space (4) of the housing and a channel outlet (45) at the upper surface (8) of the lid (5) outside the housing, wherein said cooling channel (41) has a horizontal section (46) which is at least partially oriented horizontally within the lid (5).
The invention relates to a Method of operating a process arrangement (1), in particular bioprocess arrangement, wherein the method comprises a separation of two phases of an aqueous-two-phase system (2). It is proposed that the process arrangement (1) comprises a fluidized bed centrifuge (3), that the fluidized bed centrifuge (3) is used for the separation of the two phases of the aqueous-two-phase system (2), that the aqueous-two-phase system (2) is loaded into a rotating chamber (4) of the fluidized bed centrifuge (3) as a fluid flow, that due to the rotation of the chamber (4) the aqueous-two-phase system (2) is centrifuged such that a first phase (7) of the two phases is separated 10 from a second phase (8) of the two phases, and, that due to the fluid flow during the rotation of the chamber (4), the first phase (7) remains in the chamber (4) while the second phase (8) leaves the chamber (4), such that the two phases are separated.
The invention relates to a filtration unit, in particular a syringe attachment filter or syringe filter holder, for filtering of a fluid, the filtration unit comprising: a first housing part comprising at least one first fluid chamber for a fluid to be filtered; a second housing part comprising at least one second fluid chamber for a filtered fluid; a filter element configured to fluidly connect the first chamber and the second chamber and to filter the fluid to be filtered; wherein the second housing part comprises: a support surface for supporting a fringe area of the filter element; and a rib element protruding from the support surface, at least a distal portion of the rib element consisting of or comprising a material substantially fusible by welding; wherein the first housing part comprises: an abutting surface for substantially abutting with the distal portion of the rib element, the abutting surface at least partly facing the support surface of the second housing part; and a barrier element protruding from the abutting surface towards the support surface and positioned relative to the rib element so as to laterally enclose an inner melt cavity therebetween, wherein the inner melt cavity upon welding is at least partially filled with solidified melt originating from fused material of the distal portion of the rib element, wherein the first housing part and the filter element and, optionally, the second housing part are to be coupled to one another via the solidified melt in the inner melt cavity.
B01D 27/00 - Cartridge filters of the throw-away type
B01D 29/01 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with flat filtering elements
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
The present invention relates to a porous polymer membrane having the combination of a specific average pore diameter, a specific thickness, and a specific convective porosity. Moreover, the present invention relates to a method of manufacturing such porous polymer membrane.
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Machines and machine tools for use in biopharmaceutical and laboratory manufacturing; Valves [parts of machines]; Machine couplings and connectors, except for land vehicles; Disposable machine elements for creating and separating sterile connections between apparatus of plastic, including tubes, pipes, bags, containers and filters. Laboratory apparatus and instruments; Connectors for laboratory apparatus; Couplings for laboratory apparatus; Sterile couplings for fluid handling systems; Valves for laboratory apparatus; Aseptic connectors for laboratory use; Apparatus and instruments for the sterile connection and separation of plastic vessels, including plastic tubes, pipes, bags, containers and filters; Devices for sterile fluid transfer for laboratory apparatus. Apparatus for sterilizing; Filters for fluids [parts of household or industrial installations]; Sterile valves for regulating the flow of liquids and gases for use in biopharmaceutical, pharmaceutical and laboratory installations; Disposable elements for sterile connection and separation in fluid treatment systems.
48.
METHOD OF REFERENCING AN ANALYTE MEASUREMENT IN A PURIFICATION SYSTEM
In a method of referencing an analyte measurement in a purification system during a process step in a biopharmaceutical process, the following sub-steps are performed during the same process step: guiding a medium through a purification unit where the analyte is either removed from or added to the medium; measuring at least one parameter related to the presence and/or quantity of the analyte with a first measurement system at a first measurement location upstream of the purification unit; measuring the at least one parameter with a second measurement system at a second measurement location downstream of the purification unit; and referencing the upstream measurement to the downstream measurement, or referencing the downstream measurement to the upstream measurement.
The present invention relates to a method for releasing a viral vector from a cell culture, wherein a cell of the cell culture is not required to be separated from the surrounding liquid prior to contacting the cell culture with the lysis reagent. The present invention further relates to a method for producing a viral vector inter alia comprising releasing the viral vector from a cell culture according to disclosed method for releasing a viral vector from a cell culture. The present invention further relates to a cell lysis reagent for releasing a viral vector from a cell culture. The present invention further relates to a kit for releasing a viral vector from a cell culture, wherein the kit comprises a container that contains the disclosed cell lysis reagent.
The present invention provides methods for releasing a viral vector from a cell culture. Specifically, the cell culture is contacted with a lysis reagent to generate a lysis composition and incubated, whereupon the lysis composition is contacted with an acidifying reagent to lower the pH. The method according to the present invention results in high viral vector release and reduction in co-released host cell related impurities. The present invention further provides a method for producing a viral vector. The present invention further provides a kit for releasing a viral vector from a cell culture. The present invention further provides a kit for producing a viral vector.
The invention relates to a method for controlling a bioprocess (1), wherein in a model control routine (5) a bioprocess control unit (4) controls at least one process parameter (6) of the bioprocess (1) according to a model-based control loop (7). It is proposed that during the model control routine (5) the bioprocess control unit (4) repeatedly performs a first evaluation (13) of the model-based control loop (7), that if a first decision criterion is fulfilled, the bioprocess control unit (4) switches from the model control routine (5) to a sample control routine (15), that in the sample control routine (15) the bioprocess control unit (4) controls the bioprocess (1) according to a sample-based control loop (23), that at least one sample input parameter (24) of the sample-based control loop (23) is derived from a measurement value.
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G05B 13/04 - Adaptive control systems, i.e. systems automatically adjusting themselves to have a performance which is optimum according to some preassigned criterion electric involving the use of models or simulators
G05B 17/02 - Systems involving the use of models or simulators of said systems electric
52.
METHOD OF CONTROLLING A BIOPROCESS ARRANGEMENT TO PROVIDE MEASUREMENT DATA TO A BIOPROCESS CONTROL SYSTEM
The invention relates to a method of controlling a bioprocess arrangement (1) to provide measurement data to a bioprocess control system (3), wherein the bioprocess arrangement (1) comprises a biocontainer arrangement (2), a sampling arrangement (4), a routing arrangement (6) and an analysis arrangement (7). It is proposed that the bioprocess control system (3) sends a measurement request to the control layer (9), that the measurement request contains information about a requested measurement, that the measurement request is independent of routing and analysis behind the sampling arrangement (4), that the sampling arrangement (4) draws the sample and provides the sample to the routing arrangement (6), that the control layer (9) coordinates the provision of the sample to the analysis arrangement (7) via the routing arrangement (6), that the analysis arrangement (7) sends measurement data to the control layer (9), and, that the control layer (9) sends the measurement data to the bioprocess control system (3).
DEVICE FOR ARRANGING ON A FLUID-CONDUCTING LINE AND FOR ATTACHING A FLOWMETER, AND METHOD FOR DETECTING A MEASUREMENT VARIABLE OF THE FLUID BEING CONDUCTED BY A LINE
A device for arranging on a fluid-conducting line and for attaching a flowmeter, in particular an ultrasonic flowmeter, for detecting a measurement variable of the fluid conducted by the line has a first and a second connection, and a measurement region arranged between the first connection and the second connection. The first connection, the measurement region, and the second connection define a flow path for the fluid through the device, and a flow-influencing element arranged in and/or on the flow path is configured to cause fluid flowing into the device via the first connection with a substantially laminar flow to have a substantially turbulent flow in the measurement region.
G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters
G01F 15/18 - Supports or connecting means for meters
54.
APPLICATION OF PERMITTIVITY MEASUREMENT PROBES IN AN SUSPENSION CULTURE AGGREGATE COMPRISING CELL AGGREGATES
The present disclosure relates to a method of measuring cell density in a cell suspension comprising cell aggregates, the method comprising (i) Measuring the permittivity of the cell suspension; (ii) Comparing the measured permittivity with a predetermined value that is indicative of the cell density, thereby determining the cell density. Further described is a of a permittivity probe for determining the cell density of a suspension cell culture comprising cell aggregates.
G01N 33/487 - Physical analysis of biological material of liquid biological material
G01N 27/02 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
55.
TEST PLAQUE FOR VALIDATION OF INJECTION MOULDING MATERIAL RHEOLOGICAL CHARACTERISATION
The invention relates to a test specimen (1) for rheological testing of plastic products comprising a plate body (10) from a plastic material, said plate body (10) having a first major surface (102), a second major surface opposite to the first major surface (102) and a side surface (106) connecting the first and the second major surface (104) and at least one spatial feature (12A, 12B, 14A, 14B) arranged in or on the plate body (10), each spatial feature (12A, 12B, 14A, 14B) indicative of a defect occurring in the plastic products or a geometric feature of the plastic products. The invention relates further to methods and respective systems using the test specimen, including methods and systems for testing for testing of a plastic product, for validating a rheological data set or a model of a process for manufacturing of plastic products, for determining at least one property of a plastic product to be manufactured and for manufacturing a plastic product.
The present invention concerns methods for processing and/or purification of a biological product involving multi-angle light scattering as analytical tool for process control. The methods of the invention apply multi-angle light scattering to detect the biological product, the presence of a bioburden or the functionality and/or integrity of a consumable employed in the method for processing and/or purification of the biological product. Further, the invention relates to systems for processing and/or purification of a biological product configured to perform the methods of the invention. Lastly, the invention concerns computer program products which can be used in the methods of the invention.
B01D 15/08 - Selective adsorption, e.g. chromatography
G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
57.
SYSTEM FOR DETECTING A MEASUREMENT VARIABLE OF A FLUID CONDUCTED IN A FLUID-CONDUCTING LINE, FLOW-THROUGH DEVICE FOR ARRANGING ON A FLUID-CONDUCTING LINE AND FOR ATTACHING A FLOWMETER, AND USE OF A FLOW-THROUGH DEVICE
A system for detecting a measurement variable of a fluid being conducted in a fluid-conducting line, having a flowmeter for detecting the measurement variable, a flow-through device for arranging on the fluid-conducting line and for attaching the flowmeter. The flowmeter has a first and second connection and a measurement region which is arranged between the first connection and the second connection and which can be coupled to the flowmeter. The first connection, the measurement region, and the second connection define a flow path for the fluid through the flow-through device.
G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters
G01F 15/18 - Supports or connecting means for meters
58.
BIOREACTOR FOR CELL CULTURE WITH REUSABLE REACTOR VESSEL AND OPTICAL SPECTROSCOPY INTERFACE
A bioreactor for cell cultivation including a reusable reactor vessel, preferably made of stainless steel, for receiving a fluid, and an optical spectroscopy interface including an adapter and a single-use optical spectroscopy insert. The reusable reactor vessel has a wall and a port formed in the wall. The adapter is configured to be fixed to the port in a predefined position at the port. The single-use optical spectroscopy insert is configured to be accommodated and fixed in the adapter, or the insert is an integral part of the adapter.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
59.
METHOD OF PERFORMING GAS/LIQUID-BASED INTEGRITY TEST
The present invention relates to a method of performing a gas/liquid-based integrity test, wherein air in a first membrane and a second membrane and present therebetween is replaced by a water-soluble gas before wetting the membranes. Further, the present invention relates to a method of producing an integrity-tested object having a first membrane, a second membrane and at least one boundary member.
MULTIPORT DEVICE FOR CONNECTING A LOOP TO ONE PORT OF A BIOREACTOR, AND PERFUSION OR CONCENTRATED FED-BATCH SETUP FOR PERFORMING AN UPSTREAM PROCESS OF CELL CULTURE
A multiport device for connecting a loop, preferably a tangential flow filtration loop or a sensor loop, to one port of a bioreactor, preferably a single-use bioreactor, is configured to be fixed to the port of the bioreactor. The multiport device includes a first flow path configured for withdrawing fluid from the bioreactor, and a second flow path configured for supplying fluid to the bioreactor. The first flow path has a first end adapted to be in fluid connection with the bioreactor, and a second end adapted to be connected to an inlet of the loop. The second flow path has an outer end adapted to be connected to an outlet of the loop, and a mouth adapted to be in fluid connection with the bioreactor. The mouth of the second flow path is distanced from the first end of the first flow path by at least 5 mm, preferably 10 mm.
Some aspects of the disclosure are related to systems and methods for controlled oxygen release from biomaterials in vessels and unit operations or components of cell culture, cell containment, and/or bioreactor. Vessels, unit operations, devices, and/or components of the invention may be used to perform all or part of a biological and/or chemical process involving biologicals (e.g., a plurality of cells) in the presence of oxygen-releasing agents. In some embodiments, a system comprises a vessel comprises an oxygen-releasing agent configured to generate in-situ and release oxygen in a sustained manner. The presence of an oxygen-releasing agent may advantageously allow for high cell density fermentation and cell cultivation in a vessel and provide an alternative for supplemental gassing means (e.g., sparger, etc.). Some embodiments of the disclosure are directed to employing the oxygen-releasing agents in microfluidic or millifluidic systems.
A separation system and methods for separating and purifying a target component include a separation system for separating and purifying a target component, a method for separating and purifying a target component, and the use of a single-pass crossflow diafiltration unit for integrally connecting a first and a second chromatography device.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
Containers including internal mixers and actuators assemblies and related methods are generally described. In some embodiments, a container may include a mixer and an actuator, at least one of which may be located inside of the container. The actuator may actuate the mixer to induce flow within the container and mix the contents of the container with a low volumetric footprint and high mixing efficiency. In some embodiments, the actuator may be configured to deform the mixer, which may include one or more features which may be deformed out of plane in a Kirigami or Origami fashion. The actuator and mixer may be arranged in series or in parallel. The actuator may be used without a mixer to induce flow within the container. The actuator may be driven pneumatically, hydraulically, electrically, and/or in any other suitable manner.
A bioreactor having a filter unit and a method for treating a cell broth. The filter unit has a supply channel (2), a first filter medium (4), a retentate channel (1), a second filter medium (5) and a permeate channel (3), arranged so that the first filter medium delimits the supply channel and the retentate channel from one another; and the second filter medium delimits the retentate channel and the permeate channel from one another. The supply channel is connected to an inlet for a supply medium; the retentate channel is connected to an inlet for a cell broth and to an outlet for the cell broth; the permeate channel is connected to an outlet for a permeate; and the interior of the bioreactor is connected to the inlet for the cell broth and to the outlet for the cell broth.
A tubular flow device (10) for enabling controlled flow of a fluid at a measuring point comprises an inlet flow channel (14) defining a main inflow direction, and an outlet flow channel (18) defining a main outflow direction. The flow device (10) further comprises a branching portion (22) where the inlet flow channel (14) is divided into a primary flow channel (24) and a secondary measurement channel (26), and a joining portion (36) where the primary flow channel (24) and the secondary measurement channel (26) open into the outlet flow channel (18). The measuring point is located in the secondary measurement channel (26). The primary flow channel (24) is longer than the secondary measurement channel (26) and has a constriction (42) downstream of the branching portion (22). A fluid entering the inlet flow channel (14) is divided into a primary flow flowing into the primary flow channel (24) and a secondary measurement flow flowing into the secondary measurement channel (26). The primary flow and the secondary measurement flow reach the joining portion (36) essentially at the same time, due to the Venturi effect caused by the constriction (42) of the primary flow channel (24).
B33Y 80/00 - Products made by additive manufacturing
G01F 1/661 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters using light
G01F 5/00 - Measuring a proportion of the volume flow
G01F 15/00 - Details of, or accessories for, apparatus of groups insofar as such details or appliances are not adapted to particular types of such apparatus
A METHOD OF PRODUCING DIFFERENTIATED CELLS FROM PLURIPOTENT STEM CELLS, CELL POPULATIONS OBTAINED BY THE METHOD, CORRESPONDING PHARMACEUTICAL COMPOSITIONS AND A SYSTEM FOR PRODUCING DIFFERENTIATED CELLS FROM PLURIPOTENT STEM CELLS
The present invention inter alia relates to a method of producing differentiated cells from pluripotent stem cells, comprising providing at least one multicellular 3D aggregate derived from pluripotent stem cells cultured in a first suspension culture, inducing differentiation of the cells forming the at least one multicellular 3D aggregate, allowing a continuous release of differentiated cells from the multicellular 3D aggregate into the first suspension culture; and continuously separating the released differentiated cells from the first suspension culture. The present invention also relates to a cell obtained by the method and to a pharmaceutical composition comprising the cells. The invention further relates to a corresponding system for producing differentiated cells from pluripotent stem cells.
A bioprocess assembly (10) comprises a separation system (12) and a sampling system (14). The sampling system (14) is fluidically connected to the separation system (12) and comprises a sampling membrane (32) such that the separation system (12) and the sampling membrane (32) provide a separate sampling channel (30). The sampling channel (30) comprises at least one fluidic connection (36) to receive a buffer and at least one fluidic connection (51) to a sampling station (50). Further, the sampling channel (30) comprises a counter pressure generating unit (48) and at least one sensor (40), wherein the counter pressure generating unit (48) is configured to adjust the pressure within the sampling channel (30) based on data collected by the at least one sensor (40).
A device assembly (10) for performing a production scale tangential flow filtration of a feed, preferably in a batch feed and bleed process, comprises a first loop (12) including a first tubing (20), a feed tank (22) and actuators and sensors adapted to the first tubing (20), and a second loop (14) including second tubing (28) and a filter (28), preferably a hollow fiber filter. The first loop (12) and the second loop (14) further include a common tubing section. The first loop (12) connects to the second loop (14) at a first connection point (16) upstream, or alternatively downstream, of the filter (28), and the second loop (14) connects to the first loop (12) at a second connection point (18) downstream, or alternatively upstream, of the filter (28), such that the first loop (12) supplies the feed from the feed tank (22) to the second loop (14) at the first connection point (16), or alternatively at the second connection point (18), and the second loop (14) supplies retentate to the first loop (12) at the second connection point (18) from where a part of the retentate is returned to the feed tank (22), or alternatively at the first connection point (14). At least all wetted parts of the device assembly (10) are single-use parts and the inner diameter of the first tubing (20) is smaller than the inner diameter of the second tubing (32).
C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
C12M 1/00 - Apparatus for enzymology or microbiology
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
Kirigami mixing systems and related methods are generally described. In some embodiments, a mixing system includes one or more grippers configured to deform a portion of a flexible container containing fluid when undergoing axial deformation. The grippers may include features including slots, hinges, and spines to aid in the transition of the grippers between a closed and retracted configuration when the one or more grippers are deformed. The compression of the container subsequently result in fluid flow within the container which may mix or agitate the fluid. In some embodiments, the described grippers may be formed integrally with the flexible containers. In some embodiments, the mixing system may include a plurality of grippers arranged around the flexible container.
B01F 31/55 - Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being contained in a flexible bag submitted to periodical deformation
B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
70.
METHOD OF OPERATING A BIOPROCESS ARRANGEMENT TO PERFORM AT LEAST ONE REPETITION OF A BIOPROCESS
The invention relates to a method of operating a bioprocess arrangement (1) to perform at least one repetition of a bioprocess, wherein the bioprocess arrangement (1) comprises at least one replaceable electronic component (8), wherein a process control system (7) controls the bioprocess arrangement (1), using a digital model (10). It is proposed that the replaceable electronic components (8) are removed and replaced by new electronic components (9), that the process control system (7) automatically detects the removal of the replaceable electronic components (8), automatically detects the presence of the new electronic components (9) and retrieves digital representations (11) of the new electronic components (9) based on the communication with the new electronic components (9), that the process control system (7) derives from the digital representations (11) of the replaceable electronic components (8) and the new electronic components (9) a functional relationship between the replaceable electronic components (8) and the new electronic components (9), that the process control system (7) replaces the digital representations (11) of the replaceable electronic components (8) with the digital representations (11) of the new electronic components (9) based on the functional relationship thereby updating the digital model (10), and, that the process control system (7) controls the bioprocess arrangement (1) to perform the bioprocess based 20 on the updated digital model (10).
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
G05B 17/02 - Systems involving the use of models or simulators of said systems electric
The invention relates to a flow-through centrifuge which is used, for example, for biotechnical applications, in particular as a blood centrifuge. A driving arrangement and/or transmission arrangement of the flow-through centrifuge comprises a planetary gearset. In the planetary gearset, at least one planetary belt pulley is rotatably supported on a rotating planet carrier. A torque of the planetary belt pulley is transmitted via a belt. The planet carrier and the planetary belt pulley are driven at different rotational speeds. According to the invention, the planet carrier is held by a belt tensioning unit rotating with the planet carrier. A distance of the planet carrier from a rotor axis can be changed via the belt tensioning unit in order to adjust the belt tension of the belt.
F16H 9/26 - Gearings for conveying rotary motion with variable gear ratio, or for reversing rotary motion, by endless flexible members with members having orbital motion
72.
Valve switching system for selectively interconnecting components of a bioprocess installation
A valve switching system for selectively interconnecting components of a bioprocess installation, comprising a valve switching cassette and an actuator block. It is proposed, that the valve switching cassette comprises a perforated sandwich plate with perforation holes, which sandwich plate is placed between the cassette manifold and the actuator block body.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
F16K 11/22 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit operated by separate actuating members with an actuating member for each valve, e.g. interconnected to form multiple-way valves
F16K 31/126 - Operating meansReleasing devices actuated by fluid the fluid acting on a diaphragm, bellows, or the like
73.
METHOD OF OPERATING A BIOPROCESS ARRANGEMENT TO PERFORM AT LEAST ONE REPETITION OF A BIOPROCESS
A method of operating a bioprocess arrangement to perform repetition of a bioprocess, wherein the bioprocess arrangement comprises a replaceable electronic component, wherein a process control system controls the bioprocess arrangement, using a digital model. The replaceable electronic components are removed and replaced by new electronic components, the process control system automatically detects the removal of the electronic components, automatically detects the presence of the new electronic components and retrieves digital representations of the new components, the process control system derives from the digital representations of the replaceable components and the new components a functional relationship, the process control system replaces the digital representations of the replaceable components with the digital representations of the new components based on the functional relationship thereby updating the digital model, and, that the process control system controls the bioprocess arrangement to perform the bioprocess based on the updated digital model.
A method for validating a filter unit. The method includes guiding a fluid flow of a fluid after the fluid flow passed through the filter unit to a measurement area that is connected to the filter unit. The measurement area the fluid is exposed to is at least one alternating electric field between at least two electrodes. The method includes obtaining at least one electric signal between the at least two electrodes. The at least one electric signal is at least affected by whether one or more cells of a plurality of cells in the fluid pass through the measurement area. The method includes determining, at least partially based on the obtained at least one electric signal, at least one validity information indicating a validity of the filter unit.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
Chemicals and reagents for use in media and buffer
preparation, seed train, filtration, chromatography, and
virus clearance in biotechnology and biopharmaceutical
industries; chemicals for industrial and laboratory use in
biopharmaceutical processing, including those for filtration
and purification of biological materials. Machinery and automated equipment for media and buffer
preparation, seed train, filtration, chromatography, and
polishing; industrial robots and apparatus for bioprocessing
and manufacturing including filtration systems for the
purification and separation of biological substances. Scientific, research, and laboratory apparatus for use in
biopharmaceutical manufacturing, namely, apparatus for media
and buffer preparation, seed train and production,
filtration, chromatography, polishing and virus clearance;
downloadable and recorded software for biopharmaceutical
processes, namely, software for media preparation, buffer
preparation, seed train optimization, filtration,
chromatography, polishing, and virus clearance; control
systems and software for automating biopharmaceutical
processes, namely, software for filtration and purification
processes; interfaces and human-machine interface (HMI)
software for managing and optimizing biopharmaceutical
processes; data collection and management software for
process intensification systems; devices for real-time
monitoring and optimization for biopharmaceutical
production. Industrial filtration apparatus for water, air, and gas
purification; chromatography apparatus for biopharmaceutical
purification; process systems and equipment for the
filtration, purification, and separation of biological
substances in biopharmaceutical processes, namely,
filtration systems for bioprocessing. Installation and maintenance of process intensification
systems for biopharmaceutical production.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Scientific and technological research services in the field
of biopharmaceuticals, specifically relating to bioprocess
optimization, including media and buffer preparation, seed
train, filtration, chromatography, and virus clearance;
design and development of computer software for
biopharmaceutical process automation and control; technical
consultancy services related to biopharmaceutical process
optimization, focusing on bioprocess steps namely,
filtration, chromatography, and virus clearance; software as
a service (SaaS) for biopharmaceutical manufacturing,
including media and buffer preparation, seed train,
filtration, chromatography, polishing and virus clearance;
IT services in relation to cloud-based interfaces for
managing biopharmaceutical manufacturing processes, namely,
interfaces for filtration, purification, and virus clearance
systems; testing and validation services for bioprocessing
equipment and systems; process scale-up technical consulting
and technical support for biopharmaceutical production.
Various embodiments provide a method for producing a bioproduct using a bioprocess installation. The bioprocess installation comprises an process control and a bioprocess unit. The bioprocess unit comprises a receptacle, a clarification unit with a centrifuge and a chromatography unit with a chromatograph. Cell broth obtained from the receptacle is lead through the clarification unit and the chromatography unit in a liquid stream. The clarification unit with its centrifuge is being operated in a centrifugation cycle comprising centrifugation steps. The chromatography unit with its chromatograph is being operated in a chromatography cycle comprising chromatography steps. The particle depletion can be less than 10% in particle concentration and that the execution of the steps assigned to the centrifugation cycle and the execution of the steps assigned to the chromatography cycle are at least partly being synchronized with each other by the process control in synchronization routines based on assigned synchronization strategies.
Systems and methods for characterizing a physical and solution properties of liquids are described. In some embodiments, an ultrasonic interrogation signal may be emitted into a liquid such as a solubilized protein solution. A resulting ultrasonic spectrum may be sensed and provided to a trained statistical model of the solution. The trained statistical model may then determine one or more properties of the liquid. In some embodiments, the trained statistical model determines a viscosity of the liquid and/or a solubilized protein concentration of the liquid. In some embodiments, a trained statistical classification model configured to classify the liquid based, for example, on its contents or properties is used to improve modeling accuracy.
The invention relates to a centrifuge, in particular a continuous flow centrifuge, a biotechnical centrifuge or a blood centrifuge. The centrifuge comprises a refrigerant circuit. According to the invention, the outlet side of a controllable compressor is connected to an inlet side of an evaporator via a bypass line with a valve arranged therein. In a normal operation mode, a centrifuge vessel is cooled exclusively via the control of the compressor and an expansion unit while the valve is closed. If, on the other hand, a tolerance range of the target temperature in the centrifuge vessel is left, the valve is opened in order to bring about heating and thus a return of the temperature in the centrifuge vessel to the tolerance range.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Scientific and technological research services in the field of biopharmaceuticals, specifically relating to bioprocess optimization, in particular, media and buffer preparation, cell culturing, filtration, chromatography, and virus clearance; design and development of computer software for biopharmaceutical process automation and control; technical consultancy services related to biopharmaceutical process optimization, focusing on bioprocess steps namely, filtration, chromatography, and virus clearance; software as a service (SaaS) for biopharmaceutical manufacturing, in particular, media and buffer preparation, cell culturing, filtration, chromatography, polishing and virus clearance; IT services, namely troubleshooting of software in relation to cloud-based interfaces for managing biopharmaceutical manufacturing processes, namely, interfaces for filtration, purification, and virus clearance systems; testing and validation services for bioprocessing equipment and systems; process scale-up technical consulting and technical support services in the nature of troubleshooting of computer software problems in connection with biopharmaceutical production
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Scientific and technological research services in the field of biopharmaceuticals, specifically relating to bioprocess optimization, including media and buffer preparation, seed train, filtration, chromatography, and virus clearance; design and development of computer software for biopharmaceutical process automation and control; technical consultancy services related to biopharmaceutical process optimization, focusing on bioprocess steps namely, filtration, chromatography, and virus clearance; software as a service (SaaS) for biopharmaceutical manufacturing, including media and buffer preparation, seed train, filtration, chromatography, polishing and virus clearance; IT services in relation to cloud-based interfaces for managing biopharmaceutical manufacturing processes, namely, interfaces for filtration, purification, and virus clearance systems; testing and validation services for bioprocessing equipment and systems; process scale-up technical consulting and technical support for biopharmaceutical production.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
(1) Chemicals and reagents for use in media and buffer preparation, seed train, filtration, chromatography, and virus clearance in biotechnology and biopharmaceutical industries; chemicals for industrial and laboratory use in biopharmaceutical processing, including those for filtration and purification of biological materials.
(2) Machinery and automated equipment for media and buffer preparation, seed train, filtration, chromatography, and polishing; industrial robots and apparatus for bioprocessing and manufacturing including filtration systems for the purification and separation of biological substances.
(3) Scientific, research, and laboratory apparatus for use in biopharmaceutical manufacturing, namely, apparatus for media and buffer preparation, seed train and production, filtration, chromatography, polishing and virus clearance; downloadable and recorded software for biopharmaceutical processes, namely, software for media preparation, buffer preparation, seed train optimization, filtration, chromatography, polishing, and virus clearance; control systems and software for automating biopharmaceutical processes, namely, software for filtration and purification processes; interfaces and human-machine interface (HMI) software for managing and optimizing biopharmaceutical processes; data collection and management software for process intensification systems; devices for real-time monitoring and optimization for biopharmaceutical production.
(4) Industrial filtration apparatus for water, air, and gas purification; chromatography apparatus for biopharmaceutical purification; process systems and equipment for the filtration, purification, and separation of biological substances in biopharmaceutical processes, namely, filtration systems for bioprocessing. (1) Installation and maintenance of process intensification systems for biopharmaceutical production.
01 - Chemical and biological materials for industrial, scientific and agricultural use
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
37 - Construction and mining; installation and repair services
Goods & Services
Chemicals and reagents for analyses in laboratories, other than for medical or veterinary purposes, in particular, for use in media and buffer preparation, cell culturing, filtration, chromatography, and virus clearance in biotechnology and biopharmaceutical industries; chemicals for industrial and laboratory use in biopharmaceutical processing, in particular, for filtration and purification of biological materials Machinery and automated laboratory equipment for media and buffer preparation, cell culturing, filtration, chromatography, and polishing; industrial robots and apparatus for bioprocessing and manufacturing, in particular, filtration machines for the purification and separation of biological substances Scientific, research, and laboratory apparatus for use in biopharmaceutical manufacturing, namely, apparatus for media and buffer preparation, cell culturing and production, filtration, chromatography, polishing and virus clearance; downloadable and recorded software for biopharmaceutical processes, namely, downloadable and recorded software for managing media preparation, buffer preparation, cell culturing optimization, filtration, chromatography, polishing, and virus clearance; electronic control systems for machines and downloadable and recorded software for automating biopharmaceutical processes, namely, software for operating filtration and purification processes; interfaces for computers and downloadable and recorded human-machine interface (HMI) software for managing and optimizing biopharmaceutical processes; downloadable and recorded data collection and management software for process intensification systems; electronic devices for real-time monitoring and optimization for biopharmaceutical production Industrial filtration apparatus for water, air, and gas purification; chromatography apparatus for industrial purposes, in particular for biopharmaceutical purification; process systems and equipment for the filtration, purification, and separation of biological substances in biopharmaceutical processes, namely, filtration systems for industrial purposes, in particular, for bioprocessing Installation and maintenance of process intensification apparatus for biopharmaceutical production
A device assembly including a single-use device for sensing or influencing a parameter of a running bioprocess. The single-use device is configured to have at least two different defined functional states, a first functional state being an inactive delivery state in which the single-use device is sterile and inoperable according to its intended use, and a second functional state being an active use state in which the single-use device is operable according to its intended use. The device assembly further includes a signalling element for indicating a current functional state of the single-use device to a user. Each functional state is associated with a distinct signal. The device assembly is configured such that a transfer from the first functional state to any other functional state of the single-use device is irreversible and/or permanently prevents the signalling element from indicating the signal associated with the first functional state.
A method of harvesting cell broth (12) from a mixing device (10) for mixing the cell broth (12) of a rocking motion bioreactor is shown. The mixing device (10) comprises a rocking motion platform (14), a mixing bag (18) and a processing unit (22). After a harvest process is started, the mixing bag (18) is tilted into a default harvest position and the cell broth (12) is harvested in the default harvest position of the mixing bag (18). If a pause condition is fulfilled, harvesting is paused and the mixing device starts rocking at a defined speed and a defined rocking angle for a predetermined time. After the predetermined time, harvesting in the default harvest position is resumed. Furthermore, a mixing device (10) for mixing cell broth (12) is disclosed.
A method of controlling a biopharmaceutical purification process, preferably a continuous process, making use of a separation arrangement. The separation arrangement preferably includes multiple chromatography columns or filtration devices. The method comprises the following steps: repeatedly acquiring an absorption spectrum inline or online at a fluid stream with a detector which is capable of recording spectra within a wavelength range between 190 nm and 2400 nm, preferably between 190 nm and 390 nm or 190 nm and 780 nm or 1100 nm and 2400 nm; real-time processing of the acquired spectrum; and real- time monitoring and controlling the process based on the processed spectrum. The step of processing includes at least one of the following measures: reducing noise; reducing spectral interference; correcting the acquired spectrum for scattering effects and/or other background effects.
The disclosure relates to monitoring or controlling bioprocesses by way of withdrawing discrete fluid samples from a reactor and performing on-line analysis of said sample using a fluidic manifold, for example, by way of a sensor based on label-free biomolecular interaction analysis. The disclosure relates to a system for measuring one or more analytes in discrete fluid samples from a vessel adapted to contain a fluid having one or more sensors for the measurement of the analytes in a contact volume of the fluid sample. An analysis module having at least one main sampling line in fluidic connection with the vessel and one or more pumps in fluidic connection with said main sampling line(s) and adapted to selectively pump fluid from in the main sampling line away from the vessel. At least one fluid distribution tubing in fluidic connection with the analysis module. A manifold has a body.
The invention relates to a method for automating the foam regulation during a bioprocess for cultivating cells in a bioreactor, said method comprising the following steps: determining values of one or more process variables of the bioprocess which is/are relevant for the formation of foam in the bioreactor; transferring the determined values as input signals to a controller; generating, by the controller, output signals for at least one foam regulation device depending on the input signals; and controlling the at least one foam regulation device in a requirements-oriented manner by means of the output signals output by the controller.
A coupling device (1) for coupling to a flexible diaphragm (51) of a diaphragm device (50) and comprising a base structure (10) for attaching the diaphragm device (50) thereto; an actuator structure (20) mounted movable along an actuation direction (A) relative to the base structure (10) and having a diaphragm grasping element (22) pivotably mounted between a release position and a grasping position for coupling the actuator structure (20) to the flexible diaphragm (51) in the grasping position; and a fixation structure (30) mounted movable along a fixation direction (F) relative to the base structure (10) between an open position and a closed position and designed to fix in the closed position the diaphragm grasping element (22) against leaving the grasping position.
F16K 1/48 - Attaching valve members to valve-spindles
F16K 7/16 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with flat, dished, or bowl-shaped diaphragm arranged to be deformed against a flat seat the diaphragm being mechanically actuated, e.g. by screw-spindle or cam
F16L 37/127 - Couplings of the quick-acting type in which the connection between abutting or axially-overlapping ends is maintained by locking members using hooks, pawls, or other movable or insertable locking members using hooks hinged about an axis
A membrane for microbiological analysis, a production method of a membrane for microbiological analysis, and the use of such membranes for microbiological analysis. Examples include a cellulose membrane for microbiological analysis that is impregnated with a non-ionic surfactant in an amount of from 100 ng/cm2 to 1.0 mg/cm2, with the membrane having a nominal pore size of from 0.20 μm to 0.80 μm, and a cumulative adsorption pore volume of less than 0.010 cm3/g.
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
17 - Rubber and plastic; packing and insulating materials
Goods & Services
Filter modules, namely filter capsules, filter candles,
filter cassettes, coil modules, filter coils, filter coils
in connection with filter centrifuges, band filters, small
filters, small filters in connection with centrifuge tubes
and as centrifugal concentrators, disposable filters;
membrane filters and filter matting being parts of machines,
for use in biotechnology, environmental protection, for the
drinks, food, luxury items, pharmaceutical, cosmetics,
electronics and general chemical industries, and for use in
genetic engineering, medicine and in laboratories, and for
treatment and analysis of water and effluent; filters being
parts of machines; membrane filters for use as part of
machines. Laboratory filters; filtering units for laboratory use;
liquid filter units, namely, filter cartridges, plastic
hoses and hose connectors sold as a set for laboratory use;
membranes for filtration (laboratory use); filters for use
with laboratory chromatography apparatus; membrane filters
and filter matting for laboratories with adsorption
properties, membrane adsorbers, namely adsorption membranes,
ion exchange membranes, ligand membranes and activated
membranes in the form of hollow fibre membranes, tube
membranes and flat membranes as strips or cut to size for
use in apparatus, filters and filter modules for the
aforesaid fields of application; membrane filters and filter
matting as parts of microtitre plates; membrane filters and
filter matting as parts of diagnostic tests such as
diagnostic assays and immunoassays; membrane filters and
filter matting as parts of filter systems or as independent
filters for chromatographic analyses and chemical
separation, reconcentration, for sterilisation and
ultrapurification of fluids; filters and filter modules for
filtering and reconcentration of biological solutions,
namely of viral and bacterial suspensions; filters and
filter modules for treating blood and other bodily fluids. Air filters and filtering units, for industrial use; liquid
filters and filtering units, for industrial use; filters for
filtering fluids, filter modules, filter candles and filter
capsules; liquid filter units, namely, filter cartridges,
plastic hoses and hose connectors, sold as a set, for
industrial installations; filters for water filtering
apparatus; cartridge filtration units [water treatment
equipment]; filters for use with industrial chromatography
apparatus; apparatus for water treatment and for preparing
solutions in the pharmaceutical, medical and laboratory
fields; equipment for de-ionising water; equipment for
de-pyrogenisation of solutions and for separating harmful
substances from fluids; filters and filter modules for
desalination of protein solutions and other biological
media; filters and filter modules for analysis and
separation of ions, macromolecules and biomolecules, namely
carbohydrates, peptides, proteins and nucleic acids and for
separating heavy metals and harmful substances from fluids;
filters for industrial use, namely, membrane filters and
filter matting having applications in biotechnology,
environmental protection, the drinks, food, luxury items,
pharmaceutical, cosmetics, electronics and general chemical
industries, and in genetic engineering, medicine and
laboratories, and the treatment and analysis of water and
effluents. Membranes and semi-processed synthetic filtering materials;
polymeric membranes; polymeric porous membranes in hollow
fibre form; polymeric porous membranes in sheet form;
transfer membranes of cellulose derivatives; transfer
membranes of cellulose; transfer membranes of polyamides;
transfer membranes of polyvinylidene fluoride; cross-linked
polymeric membranes in sheet form; cross-linked polymeric
membranes in hollow fibre form.
92.
METHOD FOR PERFORMING A TANGENTIAL FLOW FILTRATION ON A BIOMOLECULAR SOLUTION
The invention relates to a method for performing a tangential flow filtration on a biomolecular solution, in particular for performing a single pass tangential flow filtration on a biomolecular solution, as part of a production process for biomolecules, wherein a tangential flow filtration arrangement (1) is provided, wherein the tangential flow filtration arrangement (1) comprises a tangential flow filtration module (2), wherein the tangential flow filtration is performed inside the tangential flow filtration module (2), wherein the tangential flow filtration module (2) comprises a filter (3), a feed input fluid line (4) for the biomolecular solution as a feed medium, a retentate output fluid line (6) for a retentate, a filtrate output fluid line (7) for a filtrate, and preferably a buffer input fluid line (5) for a buffer, wherein the tangential flow filtration arrangement (1) comprises at least two actuators (8) influencing the tangential flow filtration, wherein the tangential flow filtration arrangement (1) comprises a control module (10), wherein the control module (10) controls the tangential flow filtration by providing control signals to the actuators (8) and measuring at least two measured variables (11) describing the tangential flow filtration, wherein the control module (10) uses a process control to control the tangential flow filtration. It is proposed that the process control is a model predictive control (13), that the control module (10) inputs the measured variables (11) as input variables (14) into the model and derives at least two manipulated variables (15) as control signals for the actuators (8).
The invention relates to a device for continuous virus inactivation during a protein production process, in particular an antibody production process, wherein the device comprises an axially extending hollow-body holder (1) for holding an elongated, elastically deformable hollow body (2), in particular a flexible tube, wherein the device comprises at least two axially extending rollers (3, 4), in particular three or four rollers (3, 4, 5, 6), each of which, when the device has been assembled as intended, squeezes the hollow body (2) and thereby divides the internal volume (7) of the hollow body (2) into volume portions (8) fluidically separated from one another, wherein the continuous virus inactivation is carried out in the volume portions (8) of the hollow body (2). It is proposed that the device comprises a support element (9) which, when the device has been assembled as intended, supports the lateral faces of each of the rollers (3, 4, 5, 6) in at least one point.
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
A61L 2/00 - Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lensesAccessories therefor
C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
C12M 1/00 - Apparatus for enzymology or microbiology
B01D 15/24 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
94.
METHOD OF OPERATING A BIOPROCESSING ARRANGEMENT COMPRISING A CLARIFICATION SET-UP TO REMOVE CELL DEBRIS FROM A CELL BROTH
The invention relates to a method of operating a bioprocessing arrangement (1) comprising a clarification set-up (2) to remove cell debris from a cell broth (10), wherein the clarification set-up (2) comprises a fluidized bed centrifuge (8) with at least one centrifuge chamber (11), wherein the cell broth (10) is separated into at least a waste fraction (12) and an output fraction (13), wherein the separation of the cell broth (10) into the waste fraction (12) and the output fraction (13) comprises a loading step (14) during which the centrifuge chamber (11) is loaded with cells of the cell broth (10) up to a capacity below or equal to a maximum cell loading capacity of the centrifuge chamber (11), an overloading step (15) during which the centrifuge chamber (11) is further loaded with cell broth (10), thereby flushing out cells from the centrifuge chamber (11), collecting cells flushed out during the overloading step (15) as the waste fraction (12), and collecting cells from the chamber as the output fraction (13).
09 - Scientific and electric apparatus and instruments
11 - Environmental control apparatus
Goods & Services
Laboratory filters; filtration membranes for laboratory use;
laboratory filtration apparatus and filtering materials for
the filtration of liquids and gases; filters, for use in
relation to the following goods: laboratory apparatus in the
biopharmaceutical industry; filtration systems, devices, and
membranes for laboratory use. Filters for industrial use, namely for liquids, gases, and
biopharmaceutical applications; filtration units; filtration
units, namely filter cartridges, filter capsules, filter
modules, filter capsules and disposable filters, for
industrial purposes; filters, namely small filters,
disposable filters, filter cartridges, filter modules and
diaphragm filters being parts of filtering units or being
independent filters, for sterilisation and purification of
liquids and gases in the beverage, food and general chemical
industry; filters, specifically filter cartridges and
membrane filters for single use as parts of filter systems
or as stand-alone filters for the sterilization and
purification of liquids and gases in the electronics,
pharmaceutical, food, beverage, luxury food, cosmetics and
general chemical industries.
96.
SEPARATION SYSTEM AND METHOD FOR SEPARATING AND PURIFYING A TARGET COMPONENT
A separation system for separating and purifying a target component, a method for separating and purifying a target component, and the use of a crossflow diafiltration unit for processing a target phase. The system includes an aqueous-two phase extraction unit for aqueous-two phase extraction of the fluid, a separation unit for separating a target phase, and a crossflow diafiltration unit.
A smart single-use tube line assembly for use in a unit operation of a bioprocess including at least one tube line. The tube line includes a tube body surrounding a lumen through which a medium can flow. The tube line assembly also includes a functional element embedded in the tube body, the functional element being configured to perform an optical and/or electrical function. The tube line assembly further includes an interface for coupling both the lumen and the functional element of the tube line to another component. The tube line assembly still further includes a control unit for controlling the optical and/or electrical function.
The invention relates to a an aeration device for a bioprocessing installation (2), comprising a housing (4), a circumferential rigid body (5), wherein the housing (4) comprises at least a first aeration channel (7) and a second aeration channel (8), wherein the housing (4) further comprises at least a first gas inlet port (10) and a second gas inlet port (11), and wherein the housing (4) further comprises a plurality of first gas discharge openings (12) and second gas discharge openings (13). It is proposed that the first aeration channel (7) and the second aeration channel (8) are arranged so as to overlap one another in the axial direction at least in sections.
A clamping device for fluidically and mechanically connecting two hose barbs. The clamping device includes a seal and a locking mechanism, wherein the locking mechanism can be transferred from a mounting condition to an assembled condition. In the mounting condition, the hose barbs can be inserted into the clamping device, while in the assembled condition, those hose barbs are locked in place in a position in which free ends of the hose barbs face each other and a seal coaxially aligns and fluid-tightly connects the free ends.
F16L 33/22 - Arrangements for connecting hoses to rigid membersRigid hose-connectors, i.e. single members engaging both hoses with means not mentioned in the preceding groups for gripping the hose between inner and outer parts
F16L 33/18 - Arrangements for connecting hoses to rigid membersRigid hose-connectors, i.e. single members engaging both hoses characterised by the use of additional sealing means
100.
FILTRATION DEVICE, IN PARTICULAR SMALL-SCALE FILTRATION DEVICE FOR PRODUCTION OF BIOPHARMACEUTICALS
A filtration device (10), in particular a small-scale filtration device for production of biopharmaceuticals, comprises a housing including a top cover (12) having an unfiltrate inlet (18) and a bottom cover (14) having a filtrate outlet (22), and one or more filter supports (16) stacked between the top cover (12) and the bottom cover (14) along a vertical direction such that an upper side of each filter support (16) faces the top cover (12) and a lower side of each filter support (16) faces the bottom cover (14). Each filter support (16) is equipped with a flat upper filter (40) and a flat lower filter (42). Each filter (40, 42) has a filtrate side and an opposite unfiltrate side. The upper and lower filters (40, 42) are sealed to the upper and lower sides of the filter support (16), respectively, with the filtrate sides facing the filter support (16) and the unfiltrate sides facing away from the filter support (16). Unfiltrate flow paths lead from the unfiltrate inlet of the top cover (12) to the unfiltrate side of the upper filter (40) and to the unfiltrate side of the lower filter (42), wherein blocking means prevent unfiltrate from flowing directly from the unfiltrate inlet of the top cover (12) to the filtrate outlet (22) of the bottom cover (14). Filtrate flow paths lead from the filtrate sides of the upper and lower filters (40, 42) to the filtrate outlet (22) of the bottom cover (14) via at least one filtrate guiding channel (48a, 48b) formed on the upper side of the filter support (16) and at least one filtrate guiding channel (48a, 48b) formed on the lower side of the filter support (16). The upper filter (40) and the lower filter (42) are identically shaped.
