Provided is a bone regeneration material comprising biodegradable fibers containing both β-TCP particles and m-CP particles. The bone regeneration material comprises biodegradable fibers entwined to form a cotton shape. The biodegradable fibers contain 35 to 85 wt% of tricalcium phosphate particles and 15 to 65 wt% of PLGA resin as the balance. The tricalcium phosphate contains, at a weight ratio of 5 to 50 : 95 to 50, β-TCP particles having a diameter of 0.5 to 5 μm and m-CP particles obtained by subjecting the β-TCP particles to bead milling so as thereby pulverize the β-TCP particles to a diameter of 0.1 to 0.5 μm and transform the β-TCP particles into an amorphous phase. The biodegradable fibers are spun from a spinning solution prepared using an anhydrous solvent and the m-CP particles are dispersed around the β-TCP particles within the fibers without aggregation.
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
A61L 27/12 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
Apparatus for the regeneration of stem cells for medical purposes; Biodegradable bone fixation implants; Surgical implants comprised of artificial materials; Artificial bones for implantation
3.
CELL CULTURE SUBSTRATE COMPRISING NONWOVEN MAT CONFIGURED FROM BIOCOMPATIBLE RESIN FIBERS, AND METHOD FOR PRODUCING SAME
Disclosed is a cell culture substrate including a non-woven mat formed of biocompatible fibers produced by an electrospinning method. The biocompatible fibers have a fiber length of 2 mm to 80 mm and a fiber diameter of 10 to 80 μm, and contain 20 to 50 vol % (about 45 to 75 wt %) inorganic filler particles and 50 to 80 vol % (about 25 to 55 wt %) biocompatible resin. The inorganic filler particles are partially exposed on the fiber surface to form an uneven structure. The non-woven mat can trap the seeded mesenchymal stem cells between the fibers and attach them to the fibers. A plurality of short fibers forming the non-woven mat are entangled with each other and adhered and connected at multiple points of contact, forming a three-dimensional structure in which a microenvironment in which cells can adhere and grow in the space between the biocompatible fibers and fibers.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
NATIONAL UNIVERSITY CORPORATION NAGOYA INSTITUTE OF TECHNOLOGY (Japon)
ORTHOREBIRTH CO., LTD. (Japon)
Inventeur(s)
Kasuga, Toshihiro
Nishikawa, Yasutoshi
Abrégé
A bone-regeneration material comprising a biodegradable fiber produced using an electrospinning process, wherein the biodegradable fiber comprises 30 to 50 wt % of PLLGA resin and 70 to 50 wt % of β-TCP fine particles substantially uniformly dispersed in the PLLGA resin without forming aggregates, wherein the β-TCP fine particles are not chemically bonded to the PLLGA resin, and surroundings of the β-TCP fine particles are covered by the PLLGA resin so that the β-TCP fine particles are not separated from the biodegradable fiber during a formation of the fiber, and wherein the bone-regeneration material is sterilized by using radiation sterilization with gamma ray.
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/12 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/44 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
D01D 5/00 - Formation des filaments, fils ou similaires
D01D 5/08 - Méthodes de filage à partir de masses en fusion
D01F 1/02 - Addition de substances à la solution de filage ou à la masse fondue
D01F 1/10 - Autres agents modifiant les propriétés de ces filaments
D01F 6/62 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de produits d'homopolycondensation à partir de polyesters
5.
METHOD FOR MODIFYING CRYSTALLINE CALCIUM PHOSPHATE PARTICLES INTO AMORPHOUS CALCIUM PHOSPHATE BY WET GRINDING
Provided is a method for modifying crystalline calcium phosphate particles into amorphous calcium phosphate by wet grinding using a bead mill, without causing particle agglomeration. A slurry that contains calcium phosphate particles and beads in acetone is prepared by introducing crystalline calcium phosphate particle powder and beads in quantities to make an apparent volume ratio of 1:2 to 4 into the vessel of a bead mill, introducing acetone into the mixture of calcium phosphate particle powder and beads in the vessel in a quantity to make the weight ratio of the two 1:5 to 10, and stirring. With the slurry housed in the vessel, the crystalline calcium phosphate particle powder contained in the slurry is finely ground without being caused to agglomerate, and the crystalline calcium phosphate particles are made partially amorphous.
C01B 25/32 - Phosphates de magnésium, de calcium, de strontium ou de baryum
A61L 27/12 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/42 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice inorganique
6.
METHOD FOR PRODUCING BONE REGENERATION MATERIAL HAVING COTTON-WOOL LIKE STRUCTURE
A method for producing a cotton-wool like material for bone regeneration using a wet spinning method. 50-80 wt % of calcium salt particles and 50-20 wt % of PDLLGA resin are put into a mixing vessel, dissolved in acetone, and stirred to produce a spinning solution with a resin concentration of 10-20 wt % in which said calcium salt particles are dispersed. The produced spinning solution is filled in a syringe, and the spinning solution filled in the syringe is injected into a collector container filled with poor solvent by extruding the spinning solution from the discharge port of an injection needle having a predetermined diameter. The spinning solution injected into the poor solvent is solidified into fibers by interdiffusion of desorption of organic solvent and penetration of poor solvent in the poor solvent solution. The fibers solidified in the poor solvent are deposited in a floating state in the collector vessel without fiber-to-fiber adhesion and collected in a cotton-wool like shape.
D01D 5/00 - Formation des filaments, fils ou similaires
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
D04H 1/728 - Non-tissés formés uniquement ou principalement de fibres coupées ou autres fibres similaires relativement courtes caractérisés par la méthode de formation des voiles ou couches, p. ex. par la réorientation des fibres les fibres étant disposées au hasard par électrofilage
7.
METHOD FOR CONTINUOUSLY PRODUCING BIODEGRADABLE FIBER MATERIAL CONTAINING INORGANIC FILLER PARTICLES USING WET SPINNING, AND COTTON-LIKE BONE REGENERATION MATERIAL PRODUCED WITH SAID METHOD
Provided is a method for producing biodegradable fibers that contain inorganic filler particles using wet spinning, wherein a spinning solution, which is produced by dissolving into a good solvent a mixture of inorganic filler particles and a biodegradable resin mixed at a weight ratio of 50-80:50-20 and then performing mixing, is filled into a syringe of a wet spinning device and then injected into a collector container by being pushed out downward from a discharge opening of an injection needle. A first poor solvent which has a dissolution parameter value having a small degree of separation with respect to the good solvent, and a second poor solvent which has a greater specific weight than the first poor solvent and which has a dissolution parameter value having a large degree of separation with respect to the good solvent fill the collector container in two layers vertically. The spinning solution pushed out from the discharge opening of the injection needle enters into the first poor solvent in the collector container in the form of a fiber by the weight of the spinning solution itself, and then continuously enters the second poor solvent filled beneath the first poor solvent. The spinning solution in fiber form which has entered the second poor solvent becomes a continuous long fiber and is suspended and accumulated in a cotton-like form at the bottom of the collector container.
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
D01F 6/62 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de produits d'homopolycondensation à partir de polyesters
D01F 6/92 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de mélanges de produits de polycondensation comme constituant majeur avec d'autres polymères ou des composés de bas poids moléculaire de polyesters
8.
CELL CULTURE SUBSTRATE COMPRISING NONWOVEN MAT CONFIGURED FROM BIOCOMPATIBLE RESIN FIBERS, AND METHOD FOR PRODUCING SAME
Disclosd is a cell culture substrate constituted by a nonwoven mat comprising biocompatible fibers spun by electrospinning. The biocompatible fibers have a fiber length of 2-80 mm, a fiber diameter of 10-80 μm, and include 20-50 vol% (approximately 45-75 wt%) inorganic filler particles and 50-80 vol% (approximately 25-55 wt%) biocompatible resin. The inorganic filler particles are partially exposed on the surface of the fibers to form an uneven structure. The nonwoven mat can trap seeded mesenchymal stem cells between the fibers and cause the cells to adhere to the fibers. By attaching and linking at multiple locations where multiple short fibers that constitute the nonwoven mat are entangled and in contact with each other, the nonwoven mat has a three-dimensional structure in which is formed a microenvironment in which cells can adhere and proliferate in the spaces between the biocompatible fibers.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
9.
MEHTOD FOR MANUFACTURING FLOCCULENT MATERIAL FOR BONE REGENERATION
This method for manufacturing a flocculent material for bone regeneration uses a wet spinning method and involves putting 50-80% by weight of calcium salt particles and 50-20% by weight of PDLLGA resin in a mixing container, dissolving the same in acetone and stirring the same to prepare a spinning solution in which the calcium salt particles are dispersed in the solution and which has a resin concentration of 10-20% by weight, filling a syringe with the prepared spinning solution, and extruding the spinning solution filling the syringe from a discharge port of an injection needle having a predetermined diameter to inject the same into a collector container filled with a poor solvent, wherein the spinning solution injected into the poor solvent is solidified and fiberized in the poor solvent through interdiffusion caused by desorption of an organic solvent and penetration of the poor solvent, and the fibers solidified in the poor solvent are deposited within the collector container in a floating state without adhesion between fibers and collected in a flocculent form.
A61L 27/12 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/50 - Matériaux caractérisés par leur fonction ou leurs propriétés physiques
10.
OSTEOINDUCTIVE BONE REGENERATION MATERIAL AND PRODUCTION METHOD OF THE SAME
A method of producing an osteoinductive bone graft formed of a plurality of electrospun biodegradable fibers is disclosed. The method includes preparing a fibrous scaffold material formed of the plurality of electrospun biodegradable fibers, wherein the plurality of electrospun biodegradable fibers are entangled with each other to form a cotton-wool like structure having inter-fiber spaces forming a microenvironment for cell growth therein, and immersing the fibrous scaffold in a solution containing BMP-2 so that the BMP-2 is bound to the calcium particles exposed on the surface of the fibers. Area of binding site for BMP-2 on calcium particles exposed on a surface of the electrospun biodegradable fibers is adjusted by an amount of the calcium particles contained in the electrospun biodegradable fibers.
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
D01D 5/08 - Méthodes de filage à partir de masses en fusion
D01F 1/02 - Addition de substances à la solution de filage ou à la masse fondue
D01F 6/62 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de produits d'homopolycondensation à partir de polyesters
11.
A BONE REGENERATION MATERIAL HAVING A COTTON-WOOL LIKE STRUCTURE FORMED OF A PLURALITY OF ELECTROSPUN FIBERS
A bone regeneration material has a cotton-wool like structure formed of a plurality of electrospun fibers that contain bound BMP-2 through β-TCP binding peptide. The electrospun biodegradable fiber contains 25-65 vol% of β-TCP particles distributed in the fiber such that a portion of the β-TCP particles is exposed on a surface of the electrospun fiber and the remaining portion of the β-TCP particles is buried in the fiber. β-TCP binding peptides that are fused with BMP-2 are bound to the β-TCP particles so that BMP-2 is tethered to β-TCP particles on the surface of the fibers. Upon implantation of the bone regeneration material in a bone defect site of a human body, BMP-2 that are tethered to β-TCP particles on the surface of the bone regeneration material promotes proliferation and differentiation of cells at the bone defect site.
The polymer surface of biodegradable fibers included in materials for bone regeneration has water repellency and hydrophobicity, and therefore, when such a material is implanted into an affected area, the material repels blood at a very early stage of being mixed with blood, and therefore it takes a long time for the material to be incorporated during an operation. This material for bone regeneration has a surface including hydrophilized biodegradable fibers, wherein the biodegradable fibers included in the material for bone regeneration contain osteogenic particles, and the surfaces of the biodegradable fibers are each substantially entirely covered by an amorphous calcium phosphate layer, and a portion of the calcium phosphate of the amorphous calcium phosphate layer includes carbonate apatite in which the crystal structure of calcium phosphate is replaced with calcium carbonate.
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/40 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
Provided is a cell culture substrate used for growing mesenchymal stem cells (MSC) while maintaining the differentiation potency of the mesenchymal stem cells, the cell culture substrate being manufactured on a commercial basis. The cell culture substrate comprises a nonwoven fabric made of resin fibers spun using an electrospinning method. The nonwoven fabric includes a plurality of resin fibers having outer diameters of 10-50 μm. The plurality of resin fibers are intertangled in random directions. A mesh structure is formed by the intertangled plurality of resin fibers adhering and joining together at locations where the resin fibers contact one another. The mesh structure forms mesh openings that have a substantially elliptical shape with a diameter of 100-200 μm and that are surrounded by curved fibers. Innumerable air bubble pores having diameters of 0.1-3 μm are formed over the entire surface of the fibers making up the nonwoven fabric.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
14.
PRODUCTION METHOD FOR BONE-REGENERATION MATERIAL IMPARTED WITH ANTIMICROBIAL PROPERTIES USING INOSITOL PHOSPHATE, AND ANTIMICROBIAL BONE-REGENERATION MATERIAL PRODUCED BY SAID PRODUCTION METHOD
Provided is a bone-regeneration material comprising biodegradable fibers and exhibiting antimicrobial properties at an early stage following surgery. A method for producing a bone-regeneration material having antimicrobial properties and comprising biodegradable fibers, wherein the bone-regeneration material is produced by a step in which the biodegradable fibers are immersed in an inositol phosphate solution, then subsequently immersed in a solution containing silver ions, the biodegradable fibers have an outer diameter of 10-100μm, contain at least 30 wt% or more of a biodegradable resin and 40 wt% or more of calcium compound particles, and some of the calcium compound particles are exposed on the surface of the biodegradable fibers.
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
15.
METHOD FOR ISOLATING AND EXTRACTING ADIPOSE-DERIVED STEM CELLS FROM ADIPOSE TISSUE AND CULTURING SAME WITHOUT USING COLLAGENASE, AND KIT FOR ISOLATING AND EXTRACTING ADIPOSE-DERIVED STEM CELLS
Adipose-derived stem cells are isolated and extracted from an adipose tissue without using collagenase. An adipose tissue is covered with a nonwoven fabric sheet having sufficient interfiber space and pressed by an appropriate force. Thus, the adipose tissue infiltrates among fibers of the nonwoven fabric and then comes into contact with fibers surrounding the same. By immersing the nonwoven fabric as such in a culture medium, a number of adipose-derived stem cells are allowed to crawl out from the adipose tissue and then adhered to the fiber surface.
METHOD FOR ALLOWING BIODEGRADABLE FIBROUS MATERIAL PRODUCED BY ELECTROSPINNING TO BE COLLECTED IN COTTON-LIKE FORM, AND BONE-REGENERATION MATERIAL HAVING COTTON-LIKE FORM PRODUCED WITH SAID METHOD
Conventionally, if, at a stage where fibers that float/sediment in an ethanol liquid in a collector for electrospinning are extracted from the liquid and dried, solvent remains in the fibers, the fibers become soft and do not have sufficient mechanical strength. As a result, during drying, the fibers being wet owing to ethanol cannot withstand the weights of the fibers so that the fibers flatten so as to assume a sheet-like form and not assume a cotton-like form. However, in the present invention, chloroform remaining in fibers taken out from an ethanol liquid for electrospinning is dried at low temperature and under low pressure such that the chloroform is quickly removed. Consequently, the viscosities and the hardnesses of the fibers are maintained at not lower than certain levels, whereby the fibers are prevented from having a sheet-like form but are collected in a cotton-like form.
NATIONAL UNIVERSITY CORPORATION NAGOYA INSTITUTE OF TECHNOLOGY (Japon)
ORTHOREBIRTH CO., LTD. (Japon)
YAMAHACHI DENTAL MFG., CO. (Japon)
YABASHI INDUSTRIES, CO., LTD. (Japon)
Inventeur(s)
Kasuga, Toshihiro
Ota, Yoshio
Wakita, Takashi
Abrégé
A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.
A61L 31/06 - Matériaux macromoléculaires obtenus autrement que par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 31/12 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 31/14 - Matériaux caractérisés par leur fonction ou leurs propriétés physiques
A61K 33/00 - Préparations médicinales contenant des ingrédients actifs inorganiques
C08L 67/04 - Polyesters dérivés des acides hydroxycarboxyliques, p. ex. lactones
18.
Guided bone regeneration membrane and manufacturing method thereof
NATIONAL UNIVERSITY CORPORATION NAGOYA INSTITUTE OF TECHNOLOGY (Japon)
YABASHI INDUSTRIES CO., LTD. (Japon)
YAMAHACHI DENTAL MFG., CO. (Japon)
ORTHOREBIRTH CO., LTD. (Japon)
Inventeur(s)
Kasuga, Toshihiro
Ota, Yoshio
Wakita, Takashi
Abrégé
A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.
A61K 33/00 - Préparations médicinales contenant des ingrédients actifs inorganiques
A61L 31/12 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 31/06 - Matériaux macromoléculaires obtenus autrement que par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 31/14 - Matériaux caractérisés par leur fonction ou leurs propriétés physiques
19.
Method for manufacturing bone-regeneration material comprising biodegradable fibers by using electrospinning method
National University Corporation Nagoya Institute of Technology (Japon)
Orthorebirth Co., Ltd. (Japon)
Inventeur(s)
Kasuga, Toshihiro
Nishikawa, Yasutoshi
Abrégé
7 Pa·s. A powder of calcium phosphate fine particles is added while the blade is rotated. The mixture is kneaded by continuous rotation of the blade in the heated state to disperse the calcium phosphate fine particles to obtain a composite having calcium phosphate fine particles dispersed in the PLGA resin. The composite is dissolved by a solvent, and the PLGA resin is completely dissolved by agitation for a prescribed duration to prepare a spinning solution in which the calcium phosphate fine particles are dispersed. Electrospinning is performed on the spinning solution to manufacture biodegradable fibers having therein the calcium phosphate fine particles substantially uniformly dispersed.
B29B 7/10 - MélangeMalaxage discontinu, avec dispositifs mécaniques de mélange ou de malaxage, c.-à-d. de type travaillant par charges avec dispositifs de mélange ou de malaxage mobiles rotatifs
B29B 7/12 - MélangeMalaxage discontinu, avec dispositifs mécaniques de mélange ou de malaxage, c.-à-d. de type travaillant par charges avec dispositifs de mélange ou de malaxage mobiles rotatifs avec un seul arbre
B29B 7/14 - MélangeMalaxage discontinu, avec dispositifs mécaniques de mélange ou de malaxage, c.-à-d. de type travaillant par charges avec dispositifs de mélange ou de malaxage mobiles rotatifs avec un seul arbre à vis ou à vis sans fin
B29B 7/38 - MélangeMalaxage continu, avec dispositifs mécaniques de mélange ou de malaxage avec dispositifs de mélange ou de malaxage mobiles rotatifs
B29B 7/40 - MélangeMalaxage continu, avec dispositifs mécaniques de mélange ou de malaxage avec dispositifs de mélange ou de malaxage mobiles rotatifs avec un seul arbre
B29B 7/42 - MélangeMalaxage continu, avec dispositifs mécaniques de mélange ou de malaxage avec dispositifs de mélange ou de malaxage mobiles rotatifs avec un seul arbre à vis ou à vis sans fin
C08J 3/20 - Formation de mélanges de polymères avec des additifs, p. ex. coloration
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
D01F 6/62 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de produits d'homopolycondensation à partir de polyesters
D01D 5/08 - Méthodes de filage à partir de masses en fusion
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
D01F 1/02 - Addition de substances à la solution de filage ou à la masse fondue
Surgical implants comprised of artificial materials;
artificial bone. Retail services or whole sale services for materials for
bone implants, materials for repair of bones, artificial
bone, and materials of artificial bone.
Surgical implants comprised of artificial materials;
artificial bone. Retail services or whole sale services for materials for
bone implants, materials for repair of bones, artificial
bone, and materials of artificial bone.
Apparatus for the regeneration of stem cells for medical
purposes; biodegradable bone fixation implants; surgical
implants comprised of artificial materials; artificial bone. Retail or wholesale services for pharmaceutical, veterinary
and sanitary preparations and medical supplies.
Surgical implants comprised of artificial materials; artificial bone for implantation Retail store services and wholesale store services, online retail store services and wholesale store services for materials for bone implants, materials for repair of bones, artificial bone, and materials of artificial bone
Surgical implants comprised of artificial materials; artificial bone, namely, artificial bones for implantation Wholesale and retail store services featuring materials for bone implants, materials for repair of bones, artificial bone, and materials of artificial bone; On-line wholesale and retail store services featuring materials for bone implants, materials for repair of bones, artificial bone, and materials of artificial bone
Apparatus for the regeneration of stem cells for medical purposes; biodegradable bone fixation implants; surgical implants comprised of artificial materials; artificial bone for implantation Retail store services or wholesale store services, both featuring pharmaceutical, veterinary and sanitary preparations and medical supplies
26.
METHOD FOR MANUFACTURING BONE-REGENERATION MATERIAL COMPRISING BIODEGRADABLE FIBERS BY USING ELECTROSPINNING METHOD
NATIONAL UNIVERSITY CORPORATION NAGOYA INSTITUTE OF TECHNOLOGY (Japon)
ORTHOREBIRTH CO., LTD. (Japon)
Inventeur(s)
Kasuga Toshihiro
Nishikawa Yasutoshi
Abrégé
There is a demand for a novel method for manufacturing, with commercial efficiency, a bone-regeneration material that contains calcium phosphate particles in a biodegradable fiber comprising PLGA by using electrospinning, and a novel bone-regeneration material manufactured using the method. According to the present invention, a PLGA resin is heated in a kneader so as to be softened until the viscosity of the resin becomes 102 to 107 Pa·s. A powder of calcium phosphate fine particles is poured while the blade of the kneader is being rotated, and mixed with the softened PLGA resin. The mixture is kneaded by applying thermal and mechanical energy to the mixture through the continuous rotation of the blade of the kneader in the heated state, and aggregations of the calcium phosphate fine particles are disintegrated to prepare a composite in which the calcium phosphate fine particles are dispersed in the PLGA resin. The composite is dissolved by a solvent, and the PLGA resin is completely dissolved by the solvent through agitation for a prescribed period of time to prepare a spinning solution in which the calcium phosphate fine particles are dispersed without being aggregated in a solution having the PLGA resin dissolved therein. Electrospinning is performed on the spinning solution to manufacture biodegradable fibers having therein the calcium phosphate fine particles substantially uniformly dispersed.
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/12 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
D01D 5/08 - Méthodes de filage à partir de masses en fusion
D01F 1/02 - Addition de substances à la solution de filage ou à la masse fondue
D01F 6/62 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de produits d'homopolycondensation à partir de polyesters
27.
METHOD FOR MANUFACTURING DRUG-CONTAINING BIODEGRADABLE FIBER MATERIAL BY ELECTROSPINNING
The present invention addresses the problem of providing a drug formulation material with which localized sustained release of a drug at any site in the body is possible, and which has good bioabsorption and is absorbed and broken down by the body after sustained release of the drug. A drug formulation material that has an exceedingly high sustained release effect, and that solves the foregoing problem, was successfully developed by dissolving a biodegradable resin and a drug in a solvent to prepare a spinning solution, and spinning fibers from the spinning solution by electrospinning.
A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier
A61K 47/34 - Composés macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone, p. ex. polyesters, acides polyaminés, polysiloxanes, polyphosphazines, copolymères de polyalkylène glycol ou de poloxamères
A61L 31/00 - Matériaux pour autres articles chirurgicaux
D01D 5/08 - Méthodes de filage à partir de masses en fusion
D01F 1/10 - Autres agents modifiant les propriétés de ces filaments
D01F 6/92 - Filaments, ou similaires, faits par l’homme, à un seul composant, formés de polymères synthétiquesLeur fabrication à partir de mélanges de produits de polycondensation comme constituant majeur avec d'autres polymères ou des composés de bas poids moléculaire de polyesters
D04H 1/728 - Non-tissés formés uniquement ou principalement de fibres coupées ou autres fibres similaires relativement courtes caractérisés par la méthode de formation des voiles ou couches, p. ex. par la réorientation des fibres les fibres étant disposées au hasard par électrofilage
C08J 3/11 - Production de solutions, dispersions, latex ou gel par d'autres procédés que ceux utilisant les techniques de polymérisation en solution, en émulsion ou en suspension dans des liquides organiques à partir de polymères solides
A61L 27/26 - Mélanges de matériaux macromoléculaires
A61L 27/44 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
C08K 9/02 - Ingrédients traités par des substances inorganiques
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/44 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/26 - Mélanges de matériaux macromoléculaires
NATIONAL UNIVERSITY CORPORATION NAGOYA INSTITUTE OF TECHNOLOGY (Japon)
ORTHOREBIRTH CO.LTD. (Japon)
Inventeur(s)
Kasuga Toshihiro
Makita Masashi
Abrégé
Rebuilding a bone with a defect by activation of the innate self-regeneration ability of the bone requires a considerably long period of time. The purpose of the present invention is to provide a bone defect filling material that initiates a bone rebuilding action as quickly as possible following implantation and that afterward remains in the defect and continues acting to promote bone formation activity until sufficient bone formation has been achieved for the rebuilding of the defect. The present invention provides a cotton-like bone defect filling material comprising biodegradable fibers produced by electrospinning, wherein the biodegradable fibers contain 40-60 wt% of calcium phosphate particles and 10 wt% or more of silicon-eluting calcium carbonate particles, with the remainder containing 30 wt% or more of poly(L-lactic acid) resin, and the amount of the poly(L-lactic acid) resin that is non-crystalline is 75-98%.
National University Corporation, Nagoya Institute of Technology (Japon)
Yabashi Industries Co., LTD (Japon)
Yamahachi Dental Mfg., Co (Japon)
Orthorebirth Co. Ltd. (Japon)
Inventeur(s)
Kasuga, Toshihiro
Ota, Yoshio
Wakita, Takashi
Abrégé
A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.
A61L 31/06 - Matériaux macromoléculaires obtenus autrement que par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 31/12 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 31/14 - Matériaux caractérisés par leur fonction ou leurs propriétés physiques
A61K 33/00 - Préparations médicinales contenant des ingrédients actifs inorganiques
C08K 9/02 - Ingrédients traités par des substances inorganiques
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
A61L 27/46 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire avec des charges inorganiques contenant du phosphore
C08L 67/04 - Polyesters dérivés des acides hydroxycarboxyliques, p. ex. lactones
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
A61L 27/26 - Mélanges de matériaux macromoléculaires
A61L 27/44 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent comportant une matrice macromoléculaire
A61F 2/44 - Articulations pour l'épine dorsale, p. ex. vertèbres, disques intervertébraux
A bone regeneration material has a cotton-wool like structure formed of a plurality of electrospun fibers that contain bound BMP-2 through ß-TCP binding peptide. The electrospun biodegradable fiber contains 25-65 vol% of ß-TCP particles distributed in the fiber such that a portion of the ß-TCP particles is exposed on a surface of the electrospun fiber and the remaining portion of the ß-TCP particles is buried in the fiber. ß-TCP binding peptides that are fused with BMP-2 are bound to the ß-TCP particles so that BMP-2 is tethered to ß-TCP particles on the surface of the fibers. Upon implantation of the bone regeneration material in a bone defect site of a human body, BMP-2 that are tethered to ß-TCP particles on the surface of the bone regeneration material promotes proliferation and differentiation of cells at the bone defect site.
A61K 38/17 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains
A61L 24/02 - Adhésifs ou ciments chirurgicauxAdhésifs pour dispositifs de colostomie contenant des matériaux inorganiques
A61L 24/04 - Adhésifs ou ciments chirurgicauxAdhésifs pour dispositifs de colostomie contenant des matériaux macromoléculaires
