Described is a digital microfluidics system and method for measuring an analyte concentration in a droplet. Droplet movement operations can be used to carry out biological, biochemical, and chemical reactions, measurements, and experiments and a light source and light detector or spectrophotometer can be used to transmit light through a droplet to determine the absorbance of light through the droplet to calculate a concentration of analyte in the droplet.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
2.
Digital Microfluidic Systems, Cartridges, and Methods Including Integrated Refractive Index Sensing
A digital microfluidic (DMF) system, DMF cartridge, and method including integrated refractive index (RI) sensing is disclosed. The digital microfluidic DMF system and DMF cartridge may include, for example, a RI sensor (or sensor surface) directly in the droplet operations gap of a DMF cartridge. The digital microfluidic DMF system may include, for example, the DMF cartridge, one or more illumination sources, one or more optical measurement devices, and a controller. Additionally, a method of using the DMF system and DMF cartridge that includes integrated RI sensing is provided.
The present invention is directed to an improved surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) sensors, improved SPR and LSPR sensor surface chemistry, and methods and systems for improved detection of analytes in SPR and LSPR. Use of the SPR and LSPR sensors described herein improves signal amplification, and thus, provides for higher signal-to-noise, in detection of various analytes, such as proteins, antibodies, carbohydrates, and nucleic acid molecules.
Described are devices, compounds and methods for detecting an analyte in a sample. More particularly this disclosure provides SPR and LSPR sensors modified by NTA linkers for binding a ligand and/or analyte. The NTA linkers of the disclosure typically include a head (or “anchoring site”) for coupling to a surface of the SPR or LSPR sensor, a spacer, and one or more attachment sites on a distal end or ends of the spacer which couple to a ligand. The head may include a thiol for coupling the linker to a surface of the SPR or LSPR sensor. The spacer may be a carbon chain. PAG or PEG chain, or matrix material. The one or more attachment sites may be a nitrilotriacetic acid (NTA) moiety capable of chelating a transition metal ion and binding the ligand.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
C07C 237/52 - Amides d'acides carboxyliques, le squelette carboné de la partie acide étant substitué de plus par des groupes amino ayant l'atome d'azote d'au moins un des groupes carboxamide en outre acylé
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
5.
METHODS OF SOLUTION-PHASE KINETIC ANALYSIS ON A DIGITAL MICROFLUIDIC (DMF) DEVICE
Methods of solution-phase kinetic analysis on a digital microfluidic (DMF) device are disclosed. In some embodiments, the methods of solution-phase kinetic analysis on a DMF device provide a DMF system including a DMF device (or cartridge) further including one or more electrode arrangements. The methods may utilize a variety of detection methods including surface plasmon resonance and fluorescence based techniques.
G01N 33/557 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant des mesures cinétiques, c.-à-d. mesure de l'évolution en fonction du temps de l'interaction antigène-anticorps
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/96 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir un étalon de contrôle du sang ou du sérum
6.
DEVICES AND METHODS FOR MITIGATING REACTIVE OXYGEN SPECIES IN A DIGITAL MICROFLUIDIC SYSTEM
The present disclosure provides devices and methods for reducing the effects of reactive oxygen species (ROS) in digital microfluidic (DMF) system. ROS may be reduced or entirely prevented through the introduction of ROS scavengers in the droplet operations gap of a DMF system. The mitigation of ROS in a DMF system may reduce or prevent the degradation of reagents, samples, and/or analytes as well as system components such as sensors. Additionally, this disclosure provides devices and methods for detecting and identifying ROS in a DMF system.
Sample collection and dispensing devices and methods for collecting and dispensing a sample fluid using the devices. The devices and methods may provide a plunger for holding a sample collection sponge and a flexible and/or squeezable barrel having an open end for receiving the plunger and sample collection sponge and a closed end. The plunger may include one or more flow channels operable to dispense a fluid from the barrel. A sample volume is collected using the sample collection sponge. the plunger is inserted into the barrel, and the sponge is compressed against the closed end of the barrel. causing sample liquid to flow from the sponge into the one or more flow channels in the plunger. Sample fluid may be stored in the device. The devices and methods provide a way of manually collecting, storing, and dispensing a precise amount of a sample liquid. such as saliva.
Provided herein are devices comprising: a bottom plate comprising one or more electrodes configured for performing droplet operations in a droplet operations gap formed between the bottom plate and a top plate, the top plate having a top surface comprising one or more irregularities or protrusions such the top surface is non-planar; and a cover plate configured to be disposed over the top plate, the cover plate comprising one or more alignment features for positioning the cover plate with respect to the one or more irregularities or protrusions of the top surface of the top plate.
Provided herein are digital microfluidic devices, methods and systems for improving absorbance and/or transmission detection in electromagnetic radiation spectroscopy. For example, devices and methods are provided for determining the absorbance and/or transmission of light when analyzing a fluid (e.g., a droplet) including a target analyte of interest.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
Described herein are devices and methods for fluid manipulation using electrowetting. More specifically, this disclosure provides devices and methods for manipulating fluids using a digital microfluidics (“DMF”) device. For example, a fluid may be provided to a reservoir of a DMF device. The reservoir of the DMF device may include an electrode array configured to discretize the fluid into more than one large-volume droplets, each of which may be individually manipulated within the reservoir. Any one of the more than one large-volume droplets may be primed or split into one or more medium-volume droplets. The primed droplets or medium-volume droplets may further be split into one or more small-volume droplets for further processing on the DMF device. Accordingly, the described devices and methods enable high throughput and space savings on the DMF device.
Provided herein are reagent plates, digital microfluidics (“DMF”) cartridges and systems, and methods for using the same. The reagent plate may be used for easily preparing, storing, stabilizing, transporting, and/or dispensing reagents for use with the DMF cartridges and systems. Further, the reagent plates, DMF cartridges and systems may provide easy and safe means for handling a predefined set of reagents and volumes and in a manner that may be automated, low cost, safe, and supporting easy-to-use point of case (“POC”) testing devices.
Provided herein are methods, systems and digital microfluidic cartridges for the determination of ligand-target analyte affinity value (KD), determination of an association rate constant (kon), and/or determination of a dissociation rate constant (koff) in a PR (plasmon resonance) based system. More specifically, as described herein, the present disclosure is directed to improved methods, systems and digital microfluidic cartridges for determining a dissociation rate constant (koff) using free ligand molecules in the dissociation phase or dissociation step of a standard kinetic binding curve or in ligand-target analyte affinity analysis (referred to herein as ligand assisted dissociation (LAD)).
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 21/75 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
A pipette dispenser vision system and method are disclosed. For example, a pipette dispenser vision system is provided for tracking pipettes with respect to dispensing liquid into target fluid wells, vessels, and/or reservoirs. The presently disclosed pipette dispenser vision system may include, for example, a computing device, a red/green/blue (RGB) imaging device, an infrared (IR) imaging device, an IR illumination source, and an IR sensor; all arranged with respect to, for example, a single-channel and/or a multi-channel pipette for dispensing liquid into a dispensing platform. Further, a method of using the presently disclosed pipette dispenser vision system is provided. For example, the method processes image data from the RGB imaging device and/or the IR imaging device to monitor/verify certain operations of the pipetting process and/or dispensing platform.
In some aspects, the presently disclosed subject matter provides a microfluidics system, instrument, and cartridge including multi-sided digital microfluidic (DMF) dispensing and method of use. In other aspects, the presently disclosed invention is directed to computer readable storage medium comprising instructions, which to: load a multi outlet digital microfluidic device dispenser with a liquid; dispense a first portion of the liquid from a first outlet; dispense a second portion of the liquid from a second outlet; perform a first analysis on the first portion of the liquid; perform a second analysis on the second portion of the liquid; and present results of the first analysis.
A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
There are provided systems and methods for molecular diagnostic reaction testing. The method including: receiving a patient sample in a collection device: providing the sample to a microfluidic cartridge via capillary action: inserting the microfluidic cartridge into a diagnostic device: capturing information associated with the sample: performing thermal lysis and inactivation on the sample in the microfluidic cartridge: performing sample mixing with a freeze-dried master mix by passing the sample through microfluidic channels in the microfluidic cartridge that enables mixing of the samples with the freeze-dried master mix: perform multiplex detection of different targets by passing the mixed sample into detection chambers containing probes: capturing images of the mixed samples in the detection chambers: and outputting the images.
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
20.
SMALL MOLECULE SCREENING ASSAY FOR DIGITAL MICROFLUIDIC PLATFORM
Microfluidic devices and methods for assaying molecules in aqueous droplets are provided. The methods include the steps of loading an aqueous sample onto a microfluidic device, dispensing one or more sample droplets into an oil filled droplet operations gap of the microfluidic device and optionally diluting the sample droplet, and transporting a sample droplet to a sensor zone and initiating the assay.
G01N 33/557 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant des mesures cinétiques, c.-à-d. mesure de l'évolution en fonction du temps de l'interaction antigène-anticorps
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
21.
SYSTEM AND METHOD FOR SIMULATING A LOCALIZED SURFACE PLASMON RESONANCE(LSPR) SPECTROMETER
A system and method for computationally simulating an LSPR spectrometer is described herein. The method includes reading a target peak wavelength, using a mathematical model of an LSPR spectrometer system to compute an absorbance/reflectance spectrum, using a mathematical model of an LSPR spectrometer system and an illumination source spectrum to compute an absorbed/reflected spectrum of optical dispersion, and perturbing the absorbed/reflected spectrum with imaging noise.
G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques
22.
Methods and systems for optimal capture of a multi-channel image from an LSPR spectrometer
Methods and systems for optimal capture of a multi-channel image in a LSPR spectrometry is described herein. The method comprises 1) finding a plurality of valid groupings of channels, 2) determining total capture times for each valid grouping of channels, 3) determining a measure of exposure sub-optimality, 4) estimating the expected error in peak wavelength for each valid grouping, 5) finding an optimal grouping of channels by identifying the grouping with the lowest estimate of expected error in peak wavelength and 6) capturing a subsequent multi-channel image using the optimal grouping.
A system and method for fording the peak wavelength of the spectrum sensed by an LSPR spectrometer is described herein. The method comprises reading an image representing the reflected/absorbed spectrum, using a mathematical model of the LSPR spectrometer system to estimate a parametric curve representing the absorbance/reflectance spectrum, and adjusting or optimizing the parameters of the parametric curve so as to increase the likelihood of the parametric curve representing the sensed spectrum. Also described herein is a novel method to achieve LSPR peak wavelength signal noise reduction using an adaptive regularization algorithm.
A plasmon resonance (PR) system, instrument, and/or device and configurations thereof for measuring molecular interactions is disclosed. In some embodiments, the PR system, instrument, and/or device is a localized surface plasmon resonance (LSPR) system, instrument, and/or device. In other embodiments, the PR system, instrument, and/or device is a surface plasmon resonance (SPR) system, instrument. The PR system, instrument, and/or device may include, for example, force feedback for reliable flow cell sealing, optical feedback for reliable flow cell sealing, local thermal control of an LSPR chip (e.g., a ring Peltier, a continuous Peltier), dual displacement pumps for constant flow delivery to a microfluidic device, a dual channel LSPR sensor, and any combinations thereof.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for research purposes; reagents for scientific
purposes; reagents for medical research; reagents for
chemical analyses; reagents for use in surface plasmon
resonance spectroscopy. Scientific instruments and apparatus and parts and fittings
therefor; sensors; sensor chips; biochip sensors; digital
sensors; electronic measurement sensors; parts and fittings
for all of the aforementioned goods; scientific instruments
and apparatus for use in the acquisition, capture,
generation, processing, analysis, evaluation, interrogation,
interpretation and display of scientific data; scientific
instruments and apparatus for use in surface plasmon
resonance spectroscopy; computer software for use with
scientific instruments and apparatus; computer software for
the control of surface plasmon resonance spectroscopy
instruments and apparatus; computer software for the
acquisition, capture, generation, processing, analysis,
evaluation, interrogation, interpretation and display of
scientific data; computer software for the acquisition,
capture, generation, processing, analysis, evaluation,
interrogation, interpretation and display of surface plasmon
resonance spectroscopy data; testing software; data
cartridges. Scientific and technological services; scientific
consultancy and analysis services; scientific and
technological design; leasing of scientific instruments and
apparatus; design and development of scientific instruments
and apparatus; design and development of computer software
for use with scientific instruments and apparatus;
information, advisory and consultancy services relating to
all of the aforementioned services.
26.
NITRILOTRIACETIC ACID LINKERS, SOLID PHASE SYNTHESIS OF NITRILOTRIACETIC ACID LINKERS AND APPLICATIONS THEREOF
Described are devices, compounds and methods for detecting an analyte in a sample. More particularly this disclosure provides SPR and LSPR sensors modified by NTA linkers for binding a ligand and/or analyte. The NTA linkers of the disclosure typically include a head (or "anchoring site") for coupling to a surface of the SPR or LSPR sensor, a spacer, and one or more attachment sites on a distal end or ends of the spacer which couple to a ligand. The head may include a thiol for coupling the linker to a surface of the SPR or LSPR sensor. The spacer may be a carbon chain, PAG or PEG chain, or matrix material. The one or more attachment sites may be a nitrilotriacetic acid (NTA) moiety capable of chelating a transition metal ion and binding the ligand.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
A cartridge with (a) a top substrate and a bottom substrate separated to form a droplet operations gap, the top substrate comprising a well plate comprising an array of wells each well comprising a hollow needle liquidly connecting an interior of the well with the droplet operations gap; and (b) a compressible membrane layer atop the well plate sealing the reservoirs and arranged so that compression of the flexible membrane towards the interior of the well forces a liquid from the interior of the well through the hollow needle, and into the droplet operations gap.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for research purposes; reagents for scientific purposes; reagents for medical research; reagents for chemical analyses other than for medical or veterinary purposes; reagents for scientific and research use in the nature of reagents for use in surface plasmon resonance spectroscopy Scientific instruments and apparatus and parts and fittings therefor, namely optical sensors for biochemical analysis for research or scientific purposes; biochemical sensors for detection of chemical substances for research or scientific purposes; biochemical sensor chips for detection of chemical substances for research or scientific purposes; biochip sensors for biochemical analysis for research or scientific purposes; digital biochemical sensors; electronic measurement sensors; parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; downloadable and recorded computer software for use in controlling scientific instruments and apparatus; downloadable and recorded computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; downloadable and recorded computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; downloadable and recorded computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; downloadable and recorded testing software for use with biochemical sensors for determining chemical levels Scientific and technological services, namely, creation, development, design, and use of optical sensors for biochemical analysis for research or scientific purposes; scientific consultancy and analysis services in the field of biochemical sensing for research or scientific purposes; scientific and technological design in the field of biochemical sensing for research or scientific purposes; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; design and development of computer software for use with scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services
29.
Microfluidics system, instrument, and cartridge including self-aligning optical fiber system and method
The present invention is directed to microfluidics systems, instruments, and cartridges including self-aligning optical fiber systems and methods of use thereof. More specifically, the disclosure describes a microfluidics instrument including an optical detection system, microfluidics cartridge, and a self-aligning optical fiber system capable of coupling the microfluidics instrument and the microfluidics cartridge. Further, the disclosure provides methods of optical detection operations using a microfluidics system.
Described herein are devices and methods for fluid manipulation using electrowetting. More specifically, this disclosure provides devices and methods for manipulating fluids using a digital microfluidics ("DMF") device. For example, a fluid may be provided to a reservoir of a DMF device. The reservoir of the DMF device may include an electrode array configured to discretize the fluid into more than one large-volume droplets, each of which may be individually manipulated within the reservoir. Any one of the more than one large-volume droplets may be primed or split into one or more medium-volume droplets. The primed droplets or medium-volume droplets may further be split into one or more small-volume droplets for further processing on the DMF device. Accordingly, the described devices and methods enable high throughput and space savings on the DMF device.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Reagents for research purposes; reagents for scientific purposes; reagents for medical research; reagents for chemical analyses; reagents for use in surface plasmon resonance spectroscopy.
(2) Scientific instruments and apparatus and parts and fittings therefor; sensors; sensor chips; biochip sensors; digital sensors; electronic measurement sensors; parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; computer software for use with scientific instruments and apparatus; computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; testing software; data cartridges. (1) Scientific and technological services; scientific consultancy and analysis services; scientific and technological design; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; design and development of computer software for use with scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Reagents for research purposes; reagents for scientific purposes; reagents for medical research; reagents for chemical analyses; reagents for use in surface plasmon resonance spectroscopy.
(2) Scientific instruments and apparatus and parts and fittings therefor; sensors; sensor chips; biochip sensors; digital sensors; electronic measurement sensors; parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; computer software for use with scientific instruments and apparatus; computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; testing software; data cartridges. (1) Scientific and technological services; scientific consultancy and analysis services; scientific and technological design; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; design and development of computer software for use with scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Reagents for research purposes; reagents for scientific purposes; reagents for medical research; reagents for chemical analyses; reagents for use in surface plasmon resonance spectroscopy. (1) Scientific and technological services; scientific consultancy and analysis services; scientific and technological design; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; design and development of computer software for use with scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services.
Nanostructures for improved molecular detection are disclosed. The nanostructures may be used to increase the ligand immobilization shift to increase the shift in signal of the analyte. In one embodiment, nanostructures are provided that may have small decay lengths and high sensitivity. In another embodiment, nanostructures are provided that may have large decay lengths and 3D surface matrix chemistries.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
The present invention is directed to an improved surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) sensors, improved SPR and LSPR sensor surface chemistry, and methods and systems for improved detection of analytes in SPR and LSPR. Use of the SPR and LSPR sensors described herein improves signal amplification, and thus, provides for higher signal-to-noise, in detection of various analytes, such as proteins, antibodies, carbohydrates, and nucleic acid molecules.
Provided herein are devices, systems and methods comprising a digital microfluidics (DMF) cartridge, the DMF cartridge comprising: a top plate, wherein the top plate comprises a top plate substrate; a bottom plate, wherein the bottom plate comprises a colored coverlay substrate; and a droplet operation gap between the top plate and the bottom plate.
B81B 1/00 - Dispositifs sans éléments mobiles ou flexibles, p. ex. dispositifs capillaires microscopiques
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
37.
PLASMONIC NANOPARTICLE ASSISTED ENZYME-LINKED IMMUNOSORBENT ASSAY IN A FLUIDICS DEVICE
Methods for plasmonic nanoparticle assisted detection of target analytes are provided including methods of plasmonic nanoparticle assisted enzyme-linked immunosorbent assay (ELISA) in a fluidics device. For example, a digital microfluidics (DMF) system is provided that includes a DMF device (or cartridge) in which the methods of plasmonic nanoparticle assisted ELISA may be performed. The disclosed methods for detecting target analytes include measuring an optically detectable change caused by one or a combination of etching, growth, aggregation, or altered interparticle distance of plasmonic particles in the vicinity of a target analyte-capture biomolecule complex in response to a product or byproduct generated by enzyme-substrate reactions. In the methods, the amount of enzyme-substrate reactions is proportional to the number of target analytes bound to the capture biomolecules.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
B82Y 5/00 - Nanobiotechnologie ou nanomédecine, p. ex. génie protéique ou administration de médicaments
38.
MICROFLUIDICS DEVICE AND METHOD INCLUDING BOTTOM SUBSTRATE, TOP SUBSTRATE, AND COVER PLATE
Provided herein are devices comprising: a bottom plate comprising one or more electrodes configured for performing droplet operations in a droplet operations gap formed between the bottom plate and a top plate, the top plate having a top surface comprising one or more irregularities or protrusions such the top surface is non-planar; and a cover plate configured to be disposed over the top plate, the cover plate comprising one or more alignment features for positioning the cover plate with respect to the one or more irregularities or protrusions of the top surface of the top plate.
B81B 1/00 - Dispositifs sans éléments mobiles ou flexibles, p. ex. dispositifs capillaires microscopiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G06K 7/14 - Méthodes ou dispositions pour la lecture de supports d'enregistrement par radiation électromagnétique, p. ex. lecture optiqueMéthodes ou dispositions pour la lecture de supports d'enregistrement par radiation corpusculaire utilisant la lumière sans sélection des longueurs d'onde, p. ex. lecture de la lumière blanche réfléchie
H04B 5/00 - Systèmes de transmission en champ proche, p. ex. systèmes à transmission capacitive ou inductive
A pipette dispenser vision system and method are disclosed. For example, a pipette dispenser vision system is provided for tracking pipettes with respect to dispensing liquid into target fluid wells, vessels, and/or reservoirs. The presently disclosed pipette dispenser vision system may include, for example, a computing device, a red/green/blue (RGB) imaging device, an infrared (IR) imaging device, an IR illumination source, and an IR sensor; all arranged with respect to, for example, a single-channel and/or a multi-channel pipette for dispensing liquid into a dispensing platform. Further, a method of using the presently disclosed pipette dispenser vision system is provided. For example, the method processes image data from the RGB imaging device and/or the IR imaging device to monitor/verify certain operations of the pipetting process and/or dispensing platform.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G06V 20/52 - Activités de surveillance ou de suivi, p. ex. pour la reconnaissance d’objets suspects
40.
OPTICAL ANALYSIS ON DIGITAL MICROFLUIDIC (DMF) CARTRIDGES
Provided herein are digital microfluidic devices, methods and systems for improving absorbance and/or transmission detection in electromagnetic radiation spectroscopy. For example, devices and methods are provided for determining the absorbance and/or transmission of light when analyzing a fluid (e.g., a droplet) including a target analyte of interest.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 21/35 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière infrarouge
Donoffoffoff) using free ligand molecules in the dissociation phase or dissociation step of a standard kinetic binding curve or in ligand-target analyte affinity analysis (referred to herein as ligand assisted dissociation (LAD)).
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
Provided herein are reagent plates, digital microfluidics ("DMF") cartridges and systems, and methods for using the same. The reagent plate may be used for easily preparing, storing, stabilizing, transporting, and/or dispensing reagents for use with the DMF cartridges and systems. Further, the reagent plates, DMF cartridges and systems may provide easy and safe means for handling a predefined set of reagents and volumes and in a manner that may be automated, low cost, safe, and supporting easy-to-use point of case ("POC") testing devices.
Sample collection and dispensing devices and methods for collecting and dispensing a sample fluid using the devices. The devices and methods may provide a plunger for holding a sample collection sponge and a flexible and/or squeezable barrel having an open end for receiving the plunger and sample collection sponge and a closed end. The plunger may include one or more flow channels operable to dispense a fluid from the barrel. A sample volume is collected using the sample collection sponge, the plunger is inserted into the barrel, and the sponge is compressed against the closed end of the barrel, causing sample liquid to flow from the sponge into the one or more flow channels in the plunger. Sample fluid may be stored in the device. The devices and methods provide a way of manually collecting, storing, and dispensing a precise amount of a sample liquid, such as saliva.
Provided herein is a sensing apparatus comprising, at least one LSPR light source, at least one detector, and at least one sensor for LSPR detection of a target chemical. The sensor comprises a substantially transparent, porous membrane having nanoparticles immobilized on the surface of its pores, the nanoparticles being functionalized with one or more capture molecules. There is further provided a self-referencing sensor for distinguishing non-specific signals from analyte binding signals. The self-referencing sensor comprising one or more nanoparticles having at least two distinct LSPR signals.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
The present disclosure provides a digital microfluidic (DMF) cartridge for performing a self-test for a target analyte, including a DMF cartridge comprising a bottom substrate and a top substrate separated by a droplet operations gap, wherein the bottom substrate comprises a plurality of droplet operations electrodes configured for performing droplet operations on a liquid droplet in the droplet operations gap; one or more reaction chambers or reaction zones on the bottom substrate that are supplied by an arrangement of the droplet operations electrodes, wherein each reaction chamber or reaction zone comprises at least one detection spot and is configured for performing a plasmonic particle-assisted ELISA (pELISA) for detection and quantification of a target analyte in a sample droplet. The device may include downloadable software for a self-test and be operable using a smart device.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
46.
SYSTEMS AND METHODS FOR CHARACTERIZATION OF AN ASSAY FROM REGIONS OF INTEREST USING OPTICAL REACTIONS
There are provided systems and methods for characterization of an assay from a plurality of regions of interest (ROI). The method including: receiving image data of the assay from the plurality of ROI, the image data including at least two color channels for each ROI; determining a ratio of signal change across the color channels for each ROI; converting the ratio of signal change for each well to a concentration measurement of the assay using a calibration curve, the calibration curve determined from image data of a calibration assay with known concentrations; and outputting the concentration measurement for each ROI.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
In some aspects, the presently disclosed subject matter provides a microfluidics system, instrument, and cartridge including multi-sided digital microfluidic (DMF) dispensing and method of use. In other aspects, the presently disclosed invention is directed to computer readable storage medium comprising instructions, which when executed by a processing device, causes the processing device to: load a multi outlet digital microfluidic device dispenser with a liquid; dispense a first portion of the liquid from a first outlet; dispense a second portion of the liquid from a second outlet; perform a first analysis on the first portion of the liquid; perform a second analysis on the second portion of the liquid; and present results of the first analysis.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 1/00 - ÉchantillonnagePréparation des éprouvettes pour la recherche
49.
CARTRIDGE, SYSTEM, AND METHOD FOR MOLECULAR DIAGNOSTIC REACTION TESTING
There are provided systems and methods for molecular diagnostic reaction testing. The method including: receiving a patient sample in a collection device; providing the sample to a microfluidic cartridge via capillary action; inserting the microfluidic cartridge into a diagnostic device; capturing information associated with the sample; performing thermal lysis and inactivation on the sample in the microfluidic cartridge; performing sample mixing with a freeze-dried master mix by passing the sample through microfluidic channels in the microfluidic cartridge that enables mixing of the samples with the freeze-dried master mix; perform multiplex detection of different targets by passing the mixed sample into detection chambers containing probes; capturing images of the mixed samples in the detection chambers; and outputting the images.
G01N 21/84 - Systèmes spécialement adaptés à des applications particulières
A61B 5/151 - Dispositifs de prélèvement d'échantillons de sang spécialement adaptés pour le prélèvement d'échantillons de sang capillaire, p. ex. par des lancettes
G01N 1/20 - Dispositifs pour prélever des échantillons à l'état liquide ou fluide pour matériau coulant ou s'éboulant
G01N 21/75 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
50.
DIGITAL MICROFLUIDIC (DMF) SYSTEM, DMF CARTRIDGE, AND METHOD INCLUDING INTEGRATED OPTICAL FIBER SENSING
A cartridge for use with an instrument to perform measurement of a fluid, including a digital microfluidics substrate comprising a plurality of electrowetting electrodes operative to perform droplet operations on a liquid droplet in a droplet operations gap; a top plate separated from the digital microfluidics substrate to form a droplet operations gap and comprising openings for injecting liquids into the droplet operations gap; a fiber assembly comprising a fiber optic probe projecting into the droplet operations gap and having a sensing end situated in proximity with one or more of the electrowetting electrodes.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
51.
Digital microfluidic systems, cartridges, and methods including integrated refractive index sensing
A digital microfluidic (DMF) system, DMF cartridge, and method including integrated refractive index (RI) sensing is disclosed. The digital microfluidic DMF system and DMF cartridge may include, for example, a RI sensor (or sensor surface) directly in the droplet operations gap of a DMF cartridge. The digital microfluidic DMF system may include, for example, the DMF cartridge, one or more illumination sources, one or more optical measurement devices, and a controller. Additionally, a method of using the DMF system and DMF cartridge that includes integrated RI sensing is provided.
A digital microfluidics (DMF) device including an FET-biosensor (FETB) and method of field-effect sensing is closed. In some embodiments, the DMF device may include one or more FETBs integrated into the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device may include one or more “drop-in” style FETBs in the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device, FETB, and method of field-effect sensing provide active-matrix control integrated into an active-matrix DMF device. Further, a microfluidics system for and method of using the DMF device including at least one FETB is provided.
The present invention is directed to microfluidics systems, instruments, and cartridges including self-aligning optical fiber systems and methods of use thereof.
Methods and systems for optimal capture of a multi-channel image in a LSPR spectrometry is described herein. The method comprises 1) finding a plurality of valid groupings of channels, 2) determining total capture times for each valid grouping of channels, 3) determining a measure of exposure sub-optimality, 4) estimating the expected error in peak wavelength for each valid grouping, 5) finding an optimal grouping of channels by identifying the grouping with the lowest estimate of expected error in peak wavelength and 6) capturing a subsequent multi-channel image using the optimal grouping.
A system and method for computationally simulating an LSPR spectrometer is described herein. The method includes reading a target peak wavelength, using a mathematical model of an LSPR spectrometer system to compute an absorbance/reflectance spectrum, using a mathematical model of an LSPR spectrometer system and an illumination source spectrum to compute an absorbed/reflected spectrum of optical dispersion, and perturbing the absorbed/reflected spectrum with imaging noise.
A system and method for finding the peak wavelength of the spectrum sensed by an LSPR spectrometer is described herein. The method comprises reading an image representing the reflected / absorbed spectrum, using a mathematical model of the LSPR spectrometer system to estimate a parametric curve representing the absorbance / reflectance spectrum, and adjusting or optimizing the parameters of the parametric curve so as to increase the likelihood of the parametric curve representing the sensed spectrum. Also described herein is a novel method to achieve LSPR peak wavelength signal noise reduction using an adaptive regularization algorithm.
The present invention is directed to an improved surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) sensors, improved SPR and LSPR sensor surface chemistry, and methods and systems for improved detection of analytes in SPR and LSPR. Use of the SPR and LSPR sensors described herein improves signal amplification, and thus, provides for higher signal-to-noise, in detection of various analytes, such as proteins, antibodies, carbohydrates, and nucleic acid molecules.
A cartridge with (a) a top substrate and a bottom substrate separated to form a droplet operations gap, the top substrate comprising a well plate comprising an array of wells each well comprising a hollow needle liquidly connecting an interior of the well with the droplet operations gap; and (b) a compressible membrane layer atop the well plate sealing the reservoirs and arranged so that compression of the flexible membrane towards the interior of the well forces a liquid from the interior of the well through the hollow needle, and into the droplet operations gap.
Microfluidic devices and methods for assaying molecules in aqueous droplets are provided. The methods include the steps of loading an aqueous sample onto a microfluidic device, dispensing one or more sample droplets into an oil filled droplet operations gap of the microfluidic device and optionally diluting the sample droplet, and transporting a sample droplet to a sensor zone and initiating the assay.
A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
61.
FOLDABLE DIGITAL MICROFLUIDIC (DMF) DEVICE USING FLEXIBLE ELECTRONIC PLATFORM AND METHODS OF MAKING SAME
A foldable digital microfluidic (DMF) device using a flexible electronic platform and methods of making same is disclosed. The foldable DMF device includes a flexible polyimide substrate with thin copper features that is foldable to provide opposing substrates. The foldable DMF device further includes a flexible polyimide dielectric layer also with thin copper features. In some embodiments, the structure for forming the presently disclosed foldable DMF device is based on the use of blind vias. In some embodiments, the foldable DMF device includes one droplet actuation layer. In other embodiments, the foldable DMF device includes multiple droplet actuation layers. Additionally, a method of making the foldable DMF device is provided.
Methods for plasmonic nanoparticle assisted detection of target analytes are provided including methods of plasmonic nanoparticle assisted enzyme-linked immunosorbent assay (ELISA) in a fluidics device. For example, a digital microfluidics (DMF) system is provided that includes a DMF device (or cartridge) in which the methods of plasmonic nanoparticle assisted ELISA may be performed. The disclosed methods for detecting target analytes include measuring an optically detectable change caused by one or a combination of etching, growth, aggregation, or altered interparticle distance of plasmonic particles in the vicinity of a target analyte – capture biomolecule complex in response to a product or byproduct generated by enzyme-substrate reactions. In the methods, the amount of enzyme-substrate reactions is proportional to the number of target analytes bound to the capture biomolecules.
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
B82Y 30/00 - Nanotechnologie pour matériaux ou science des surfaces, p. ex. nanocomposites
B82Y 40/00 - Fabrication ou traitement des nanostructures
G01N 19/06 - Recherche par enlèvement de matière, p. ex. test à l'étincelle
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
A pipette dispenser vision system and method are disclosed. For example, a pipette dispenser vision system is provided for tracking pipettes with respect to dispensing liquid into target fluid wells, vessels, and/or reservoirs. The presently disclosed pipette dispenser vision system may include, for example, a computing device, a red/green/blue (RGB) imaging device, an infrared (IR) imaging device, an IR illumination source, and an IR sensor; all arranged with respect to, for example, a single-channel and/or a multi-channel pipette for dispensing liquid into a dispensing platform. Further, a method of using the presently disclosed pipette dispenser vision system is provided. For example, the method processes image data from the RGB imaging device and/or the IR imaging device to monitor/verify certain operations of the pipetting process and/or dispensing platform.
G01N 1/14 - Dispositifs d'aspiration, p. ex. pompesDispositifs d'éjection
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
64.
Plasmon resonance system, instrument, and device for measuring molecular interactions
A plasmon resonance (PR) system, instrument, and/or device and configurations thereof for measuring molecular interactions is disclosed. In some embodiments, the PR system, instrument, and/or device is a localized surface plasmon resonance (LSPR) system, instrument, and/or device. In other embodiments, the PR system, instrument, and/or device is a surface plasmon resonance (SPR) system, instrument. The PR system, instrument, and/or device may include, for example, force feedback for reliable flow cell sealing, optical feedback for reliable flow cell sealing, local thermal control of an LSPR chip (e.g., a ring Peltier, a continuous Peltier), dual displacement pumps for constant flow delivery to a microfluidic device, a dual channel LSPR sensor, and any combinations thereof.
The present disclosure provides a digital microfluidic (DMF) cartridge for performing a self-test for a target analyte, including a DMF cartridge comprising a bottom substrate and a top substrate separated by a droplet operations gap, wherein the bottom substrate comprises a plurality of droplet operations electrodes configured for performing droplet operations on a liquid droplet in the droplet operations gap; one or more reaction chambers or reaction zones on the bottom substrate that are supplied by an arrangement of the droplet operations electrodes, wherein each reaction chamber or reaction zone comprises at least one detection spot and is configured for performing a plasmonic particle-assisted ELISA (pELISA) for detection and quantification of a target analyte in a sample droplet. The device may include downloadable software for a self-test and be operable using a smart device.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 33/564 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour complexes immunologiques préexistants ou maladies auto-immunes
66.
Plasmon resonance (PR) system, instrument, cartridge, and methods and configurations thereof
A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
67.
DIGITAL MICROFLUIDIC (DMF) SYSTEM, DMF CARTRIDGE, AND METHOD INCLUDING INTEGRATED OPTICAL FIBER SENSING
A cartridge for use with an instrument to perform measurement of a fluid, including a digital microfluidics substrate comprising a plurality of electrowetting electrodes operative to perform droplet operations on a liquid droplet in a droplet operations gap; a top plate separated from the digital microfluidics substrate to form a droplet operations gap and comprising openings for injecting liquids into the droplet operations gap; a fiber assembly comprising a fiber optic probe projecting into the droplet operations gap and having a sensing end situated in proximity with one or more of the electrowetting electrodes.
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p. ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
G01N 21/01 - Dispositions ou appareils pour faciliter la recherche optique
G01N 21/63 - Systèmes dans lesquels le matériau analysé est excité de façon à ce qu'il émette de la lumière ou qu'il produise un changement de la longueur d'onde de la lumière incidente excité optiquement
68.
DIGITAL MICROFLUIDIC SYSTEMS, CARTRIDGES, AND METHODS INCLUDING INTEGRATED REFRACTIVE INDEX SENSING
A digital microfluidic (DMF) system, DMF cartridge, and method including integrated refractive index (RI) sensing is disclosed. The digital microfluidic DMF system and DMF cartridge may include, for example, a RI sensor (or sensor surface) directly in the droplet operations gap of a DMF cartridge. The digital microfluidic DMF system may include, for example, the DMF cartridge, one or more illumination sources, one or more optical measurement devices, and a controller. Additionally, a method of using the DMF system and DMF cartridge that includes integrated RI sensing is provided.
G01N 21/41 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique
B81B 7/02 - Systèmes à microstructure comportant des dispositifs électriques ou optiques distincts dont la fonction a une importance particulière, p. ex. systèmes micro-électromécaniques [SMEM, MEMS]
69.
DIGITAL MICROFLUIDICS (DMF) DEVICE INCLUDING AN FET-BIOSENSOR (FETB) AND METHOD OF FIELD-EFFECT SENSING
A digital microfluidics (DMF) device including an FET-biosensor (FETB) and method of field-effect sensing is disclosed. In some embodiments, the DMF device may include one or more FETBs integrated into the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device may include one or more "drop-in" style FETBs in the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device, FETB, and method of field-effect sensing provide active-matrix control integrated into an active-matrix DMF device. Further, a microfluidics system for and method of using the DMF device including at least one FETB is provided.
Provided herein is a sensing apparatus comprising, at least one LSPR light source, at least one detector, and at least one sensor for LSPR detection of a target chemical. The sensor comprises a substantially transparent, porous membrane having nanoparticles immobilized on the surface of its pores, the nanoparticles being functionalized with one or more capture molecules. There is further provided a self-referencing sensor for distinguishing non-specific signals from analyte binding signals. The self-referencing sensor comprising one or more nanoparticles having at least two distinct LSPR signals.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for research purposes; reagents for scientific
purposes; reagents for medical research; reagents for
chemical analyses; reagents for use in surface plasmon
resonance spectroscopy. Scientific instruments and apparatus and parts and fittings
therefor; sensors; sensor chips; biochip sensors; digital
sensors; electronic measurement sensors; parts and fittings
for all of the aforementioned goods; scientific instruments
and apparatus for use in the acquisition, capture,
generation, processing, analysis, evaluation, interrogation,
interpretation and display of scientific data; scientific
instruments and apparatus for use in surface plasmon
resonance spectroscopy; computer software for use with
scientific instruments and apparatus; computer software for
the control of surface plasmon resonance spectroscopy
instruments and apparatus; computer software for the
acquisition, capture, generation, processing, analysis,
evaluation, interrogation, interpretation and display of
scientific data; computer software for the acquisition,
capture, generation, processing, analysis, evaluation,
interrogation, interpretation and display of surface plasmon
resonance spectroscopy data; testing software; data
cartridges. Scientific and technological services; scientific
consultancy and analysis services; scientific and
technological design; leasing of scientific instruments and
apparatus; design and development of scientific instruments
and apparatus; design and development of computer software
for use with scientific instruments and apparatus;
information, advisory and consultancy services relating to
all of the aforementioned services.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for research purposes; reagents for scientific
purposes; reagents for medical research; reagents for
chemical analyses; reagents for use in surface plasmon
resonance spectroscopy. Scientific instruments and apparatus and parts and fittings
therefor; sensors; sensor chips; biochip sensors; digital
sensors; electronic measurement sensors; parts and fittings
for all of the aforementioned goods; scientific instruments
and apparatus for use in the acquisition, capture,
generation, processing, analysis, evaluation, interrogation,
interpretation and display of scientific data; scientific
instruments and apparatus for use in surface plasmon
resonance spectroscopy; computer software for use with
scientific instruments and apparatus; computer software for
the control of surface plasmon resonance spectroscopy
instruments and apparatus; computer software for the
acquisition, capture, generation, processing, analysis,
evaluation, interrogation, interpretation and display of
scientific data; computer software for the acquisition,
capture, generation, processing, analysis, evaluation,
interrogation, interpretation and display of surface plasmon
resonance spectroscopy data; testing software; data
cartridges. Scientific and technological services; scientific
consultancy and analysis services; scientific and
technological design; leasing of scientific instruments and
apparatus; design and development of scientific instruments
and apparatus; design and development of computer software
for use with scientific instruments and apparatus;
information, advisory and consultancy services relating to
all of the aforementioned services.
73.
FOLDABLE DIGITAL MICROFLUIDIC (DMF) DEVICE USING FLEXIBLE ELECTRONIC PLATFORM AND METHODS OF MAKING SAME
A foldable digital microfluidic (DMF) device using a flexible electronic platform and methods of making same is disclosed. The foldable DMF device includes a flexible polyimide substrate with thin copper features that is foldable to provide opposing substrates. The foldable DMF device further includes a flexible polyimide dielectric layer also with thin copper features. In some embodiments, the structure for forming the presently disclosed foldable DMF device is based on the use of blind vias. In some embodiments, the foldable DMF device includes one droplet actuation layer. In other embodiments, the foldable DMF device includes multiple droplet actuation layers. Additionally, a method of making the foldable DMF device is provided.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for research purposes; reagents for scientific purposes; reagents for chemical analyses other than for medical or veterinary purposes; reagents for scientific and research use in the nature of reagents for use in surface plasmon resonance spectroscopy Scientific instruments and apparatus and parts and fittings therefor, namely, optical sensors for biochemical analysis for research or scientific purposes; biochemical sensors for detection of chemical substance for research or scientific purposes; biochemical sensor chips for detection of chemical substance for research or scientific purposes; biochip sensors for biochemical analysis for research or scientific purposes; digital biochemcial sensors for detection of chemical substance for research or scientific purposes; electronic measurement sensors; structural parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; Downloadable or recorded computer software for use with scientific instruments and apparatus; Downloadable or recorded computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; Downloadable or recorded computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; Downloadable or recorded computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; Downloadable or recorded testing software for use with biochemical sensors; data cartridges in the nature of blank data storage cartridges for scientific data Scientific and technological services, namely, creation, development, design, and use of optical sensors for biochemical analysis for research or scientific purposes; scientific consultancy and analysis services in the field of biochemical sensing for research or scientific purposes; scientific and technological design in the field of biochemical sensing for research or scientific purposes; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for research purposes; reagents for scientific purposes; reagents for chemical analyses other than for medical or veterinary purposes; reagents for scientific and research use in the nature of reagents for use in surface plasmon resonance spectroscopy Scientific instruments and apparatus and parts and fittings therefor, namely, optical sensors for biochemical analysis for research or scientific purposes; biochemical sensors for detection of chemical substance for research or scientific purposes; biochemical sensor chips for detection of chemcial substance for research or scientific purposes; biochip sensors for biochemcial analysis for research or scientific purposes; digital biochemical sensors for detection of chemcial substance for research or scientific purposes; electronic measurement sensors; structural parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; Downloadable or recorded computer software for use with scientific instruments and apparatus; Downloadable or recorded computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; Downloadable or recorded computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; Downloadable or recorded computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; Downloadable or recorded testing software for use with biochemical sensors; data cartridges in the nature of blank data storage cartridges for scientific data Scientific and technological services, namely, creation, development, design, and use of optical sensors for biochemical analysis for research or scientific purposes; scientific consultancy and analysis services in the field of biochemical sensing for research or scientific purposes; scientific and technological design in the field of biochemical sensing for research or scientific purposes; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Reagents for research purposes; reagents for scientific purposes; reagents for medical research; reagents for chemical analyses; reagents for use in surface plasmon resonance spectroscopy.
(2) Scientific instruments and apparatus and parts and fittings therefor; sensors; sensor chips; biochip sensors; digital sensors; electronic measurement sensors; parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; computer software for use with scientific instruments and apparatus; computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; testing software; data cartridges. (1) Scientific and technological services; scientific consultancy and analysis services; scientific and technological design; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; design and development of computer software for use with scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services.
77.
PLASMON RESONANCE SYSTEM, INSTRUMENT, AND DEVICE FOR MEASURING MOLECULAR INTERACTIONS
A plasmon resonance (PR) system, instrument, and/or device and configurations thereof for measuring molecular interactions is disclosed. In some embodiments, the PR system, instrument, and/or device is a localized surface plasmon resonance (LSPR) system, instrument, and/or device. In other embodiments, the PR system, instrument, and/or device is a surface plasmon resonance (SPR) system, instrument. The PR system, instrument, and/or device may include, for example, force feedback for reliable flow cell sealing, optical feedback for reliable flow cell sealing, local thermal control of an LSPR chip (e.g., a ring Peltier, a continuous Peltier), dual displacement pumps for constant flow delivery to a microfluidic device, a dual channel LSPR sensor, and any combinations thereof.
Nanostructures for improved molecular detection are disclosed. The nanostructures may be used to increase the ligand immobilization shift to increase the shift in signal of the analyte. In one embodiment, nanostructures are provided that may have small decay lengths and high sensitivity. In another embodiment, nanostructures are provided that may have large decay lengths and 3D surface matrix chemistries.
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p. ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
79.
PLASMON RESONANCE (PR) SYSTEM AND INSTRUMENT, DIGITAL MICROFLUIDIC (DMF) CARTRIDGE, AND METHODS OF USING LOCALIZED SURFACE PLASMON RESONANCE (LSPR) FOR ANALYSIS OF ANALYTES
A plasmon resonance (PR) system and instrument, digital microfluidic (DMF) cartridge, and methods of using localized surface plasmon resonance (LSPR) and droplet operations for analysis of analytes is disclosed. For example, a PR system is provided that may include a DMF cartridge that may support both fixed LSPR sensing capability and in-solution LSPR sensing capability for analysis of analytes. The DMF cartridge may include an electrode arrangement for performing droplet operations, wherein the droplet operations can be used for performing fixed LSPR sensing operations and in-solution LSPR sensing operations. Further, methods of using droplet operations in the DMF cartridge to perform fixed LSPR sensing operations and/or in-solution LSPR sensing operations are provided.
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p. ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
G01N 1/00 - ÉchantillonnagePréparation des éprouvettes pour la recherche
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Reagents for research purposes; reagents for scientific purposes; reagents for medical research; reagents for chemical analyses; reagents for use in surface plasmon resonance spectroscopy.
(2) Scientific instruments and apparatus and parts and fittings therefor; sensors; sensor chips; biochip sensors; digital sensors; electronic measurement sensors; parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; computer software for use with scientific instruments and apparatus; computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; testing software; data cartridges. (1) Scientific and technological services; scientific consultancy and analysis services; scientific and technological design; leasing of scientific instruments and apparatus; design and development of scientific instruments and apparatus; design and development of computer software for use with scientific instruments and apparatus; information, advisory and consultancy services relating to all of the aforementioned services.
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Scientific instruments and apparatus and parts and fittings therefor; sensors; sensor chips; biochip sensors; digital sensors; electronic measurement sensors; parts and fittings for all of the aforementioned goods; scientific instruments and apparatus for use in the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; scientific instruments and apparatus for use in surface plasmon resonance spectroscopy; computer software for use with scientific instruments and apparatus; computer software for the control of surface plasmon resonance spectroscopy instruments and apparatus; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of scientific data; computer software for the acquisition, capture, generation, processing, analysis, evaluation, interrogation, interpretation and display of surface plasmon resonance spectroscopy data; testing software; data cartridges.
82.
PLASMON RESONANCE (PR) SYSTEM, INSTRUMENT, CARTRIDGE, AND METHODS AND CONFIGURATIONS THEREOF
A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.
G01N 1/00 - ÉchantillonnagePréparation des éprouvettes pour la recherche
B81B 1/00 - Dispositifs sans éléments mobiles ou flexibles, p. ex. dispositifs capillaires microscopiques
G01N 21/01 - Dispositions ou appareils pour faciliter la recherche optique
G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique
Provided herein is a sensing apparatus comprising, at least one LSPR light source, at least one detector, and at least one sensor for LSPR detection of a target chemical. The sensor comprises a substantially transparent, porous membrane having nanoparticles immobilized on the surface of its pores, the nanoparticles being functionalized with one or more capture molecules. There is further provided a self-referencing sensor for distinguishing non-specific signals from analyte binding signals. The self-referencing sensor comprising one or more nanoparticles having at least two distinct LSPR signals.
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
G01N 15/06 - Recherche de la concentration des suspensions de particules
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
Provided herein is a sensing apparatus comprising, at least one LSPR light source, at least one detector, and at least one sensor for LSPR detection of a target chemical. The sensor comprises a substantially transparent, porous membrane having nanoparticles immobilized on the surface of its pores, the nanoparticles being functionalized with one or more capture molecules.
G01N 15/06 - Recherche de la concentration des suspensions de particules
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
