2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12P 39/00 - Procédés faisant intervenir simultanément des micro-organismes de différents genres dans le même procédé
Apparatuses and associated methods are described for the efficient evaluation of C1-containing substrates, and especially for such evaluation conducted locally, or on-site, at a prospective facility for implementation of a biological conversion process for desired end product using a C1 carbon source. The exact composition of a given, industrial C1-containing substrate, as well as the range in composition fluctuations, are generally difficult to reproduce at a remote facility (e.g., a laboratory or a pilot-scale or demonstration-scale process), as required for the accurate prediction/modeling of commercial performance to justify large capital expenditures for commercial scale-up.
C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
C12M 1/107 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens pour recueillir les gaz de fermentation, p. ex. le méthane
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
G01N 30/46 - Modèles d'écoulement utilisant plus d'une colonne
G01N 30/00 - Recherche ou analyse de matériaux par séparation en constituants utilisant l'adsorption, l'absorption ou des phénomènes similaires ou utilisant l'échange d'ions, p. ex. la chromatographie
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
4.
Microbial fermentation for the production of terpenes
The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.
C12P 5/02 - Préparation des hydrocarbures acycliques
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
Improvements in biological conversion processes and associated apparatuses are disclosed for the generation of useful end products such as ethanol, through metabolic pathways of C1-fixing bacteria that utilize, as a nutrient, a C1-carbon source from a C1-containing substrate such as an industrial waste gas. Particular aspects of the disclosure relate to the downstream recovery of ethanol and/or isopropanol from bleed and permeate streams and more particularly to performing such recovery with improved efficiency that can advantageously reduce capital (e.g., equipment) and/or operating (e.g., utility) costs. Particular aspects related to the downstream recovery of ethanol and/or isopropanol using a low pressure separator having an internal divider.
Improvements in biological conversion processes and associated apparatuses are disclosed for the generation of useful end products such as ethanol, through metabolic pathways of C1-fixing bacteria that utilize, as a nutrient, a C1-carbon source from a C1-containing substrate such as an industrial waste gas. Particular aspects of the disclosure relate to the downstream recovery of ethanol and/or isopropanol from bleed and permeate streams and more particularly to performing such recovery with improved efficiency that can advantageously reduce capital (e.g., equipment) and/or operating (e.g., utility) costs.
2 generated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process.
The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways.
C12N 1/38 - Stimulation chimique de la croissance ou de l'activité par addition de composés chimiques qui ne sont pas des facteurs essentiels de croissanceStimulation de la croissance par élimination d'un composé chimique
C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.
The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
Clostridium bacterium that expresses one or more of pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), acetolactate synthase (EC 2.2.1.6), and acetolactate decarboxylase (EC 4.1.1.5). The invention further provides a method of producing a fermentation product by fermenting the recombinant bacterium in the presence of a gaseous substrate comprising CO to produce one or more of ethanol, butanol, isopropanol, isobutanol, higher alcohols, butanediol, 2,3-butanediol, succinate, isoprenoids, fatty acids, biopolymers, and mixtures thereof.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12P 39/00 - Procédés faisant intervenir simultanément des micro-organismes de différents genres dans le même procédé
Multi-stage, biological processes and systems for converting a C1 carbon source to desired end products are described. The processes comprise dividing a gaseous C1-containing substrate, in parallel, among multiple bioreactor stages. Liquid products are successively fed, in series, from a first bioreactor stage to downstream bioreactor stages. Operation can be simplified by avoiding the requirement for microorganism separation and recycle at each stage. In addition, overall vapor-liquid mass transfer for the combined stages is very favorable, leading to high end product productivity with comparably low byproduct metabolite productivity.
The invention provides genetically engineered microorganisms with altered carbon monoxide dehydrogenase (CODH) activity and methods related thereto. In particular, the invention provides a genetically engineered carboxydotrophic acetogenic bacterium having decreased or eliminated activity of CODH1 and/or CODH2. In certain embodiments, the bacterium may also have increased activity of CODH/ACS. The invention further provides a method for producing a product by culturing the bacterium in the presence of a gaseous substrate comprising one or more of carbon monoxide, carbon dioxide, and hydrogen.
Processes, as well as associated systems and computer program (software) products, are disclosed for the biological conversion of CO into desired end products such as ethanol. The control methodologies used for these processes can advantageously result in a reduced time required for a batch operation or other initial operating period, prior to achieving a continuous operation, which may be demarcated either by the addition of fresh culture medium at a defined flow rate or by another process initiation target. The control methodologies may alternatively, or in combination, improve a process performance parameter, such as productivity of the desired end product or bacterial growth rate, during this batch operation or other initial operating period.
A process for producing and controlling pyruvate derived products during the fermentation of a CO containing substrate by an acetogenic carboxydotrophic microorganism has been developed. The process involves increasing the concentration of at least one nutrient selected from the group consisting of vitamin B1, vitamin B5, vitamin B7 and mixtures thereof above the cellular requirement of the microorganism. When the concentration is increased, the production of 2,3-butanediol (2,3-BDO) increases whereas the production of the other metabolites is virtually unchanged. The effect is reversible so that when the concentration is decreased, the production of 2,3-BDO is also decreased. This allows one to control the ratio of ethanol:2,3-BDO to a desired value which can vary from about 4:1 to about 1:2.
This invention relates generally to method for producing products, particularly alcohols, by microbial fermentation. In particular, the invention relates to methods for increasing the efficiency of the fermentation, by providing a method for treating the used fermentation broth to produce a treated permeate which is then passed back to the bioreactor. The invention provides a method whereby at least one treatment step used to treat the permeated, produces a gaseous product which is then used in one or more stages of the fermentation process.
The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.
C12P 7/04 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle acycliques
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
The invention provides a genetic tool to insert, replace, delete, or otherwise manipulate a nucleic acid sequence in a microorganism to produce a recombinant microorganism. Notably, the invention makes use of homologous recombination, a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. Since the invention involves three homologous recombination events, it is referred to as a “triple cross” method.
C12N 15/66 - Méthodes générales pour insérer un gène dans un vecteur pour former un vecteur recombinant, utilisant le clivage et la ligatureUtilisation de linkers non fonctionnels ou d'adaptateurs, p. ex. linkers contenant la séquence pour une endonucléase de restriction
C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12P 7/08 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques
A methods for altering the metabolite profile of a fermentation, by increasing flux through acetolactate. The methods comprises increasing production of one or more products derived from acetolactate. Further provided is a method for increasing the production of 2,3-butandiol by microbial fermentation of gaseous substrates, the method comprising providing a compound which inhibits one or more enzymes which convert acetolactate to branched chain amino acids to the fermentation. The present invention further provides methods for increasing the production of 2,3-butandiol relative to other fermentation products such as ethanol and acetic acid.
A bioreactor system is provided for continuous fermentation of a gaseous substrate, said system comprising two or more primary bioreactors and one or more secondary bioreactors connected by a central bleed line. Further provided is a process for inoculating multiple bioreactors utilizing a central bleed line, said process comprising passing fermentation broth from a first primary bioreactor to other primary bioreactors and/or secondary bioreactors via a central bleed line. Further provided is a process for maintaining stable fermentation of a gaseous substrate across multiple bioreactors, said process comprising providing fermentation broth from one or more operational primary bioreactors to one or more secondary bioreactors via a central bleed line.
A reactor system is provided for improved fermentation of a gaseous substrate through the introduction of a secondary loop to a forced-circulation loop reactor. The reactor comprises a primary loop through which fermentation broth comprising a gaseous substrate is circulated through a riser segment and a downcomer section by a loop pump. Downstream of the loop pump a portion of fermentation broth is withdrawn from the downcomer section and is directed to the top of the reactor via a secondary loop. Further provided is a method for improving the mass transfer of a gaseous substrate to a fermentation broth in a fermentation vessel comprising a secondary loop. Further provided is a method for reducing foam in the headspace of a fermentation vessel comprising a secondary loop.
Processes, as well as associated systems, are disclosed for the biological conversion of CO into desired end products such as ethanol. The use of a plurality of perforated plates, for example in the riser section of a bioreactor, which are positioned substantially horizontally and normal to the upward flow of both a CO-containing substrate and liquid culture medium, can significantly improve CO utilization of the bacteria and consequently the overall process economics. The geometry of apertures in the perforated plates is an important determinant of their performance, with fractal patterns and other multi-edged shapes leading to particularly advantageous results.
The invention provides a recombinant carboxydotrophic Clostridia microorganism with increased overall utilization of NADPH relative to a parent microorganism. Further, the invention provides a method of producing a recombinant carboxydotrophic Clostridia microorganism which exhibits increased NADPH utilization relative to a parental microorganism. In particular, the invention relates to increasing the overall utilization of NADPH in a recombinant carboxydotrophic Clostridia microorganism in order to increase the production of at least one fermentation product by the microorganism.
The invention provides a microbubble generation system with increased efficiency and flexibility compared to known systems. Further, the invention provides a method of microbubble generation. In particular, invention relates to increasing the efficiency of a fermentation reaction by reducing bubble size and increasing gas absorption into a liquid fermentation broth.
B28C 5/06 - Appareillages ou procédés pour la production de mélanges de ciment avec d'autres substances, p. ex. coulis, mortiers, compositions poreuses ou fibreuses n'utilisant pas de moyens mécaniques d'entraînement pour effectuer le mélange le mélange étant effectué par l'action d'un fluide
B01F 3/04 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de gaz ou de vapeurs avec des liquides
B01F 13/10 - Installations pour effectuer des mélanges comprenant des combinaisons de mélangeurs de types différents
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
32.
Recombinant microorganisms and methods of use thereof
The invention relates to methods for the production of chemical compounds, particularly but not exclusively ethanol, by microbial fermentation. Also described are genetically modified micro-organisms capable of using carbon monoxide to produce one or more products, particularly but not exclusively ethanol as a main product, and producing a reduced amount or substantially no 2,3-butanediol and/or a precursor thereof.
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The invention relates, inter alia, to novel genetically modified microorganisms capable of using CO to produce 1-butanol and/or a precursor thereof, novel methyltransferases and nucleic acids encoding same, methods for producing genetically modified microorganisms using said novel methyltransferases, and methods of producing 1-butanol and/or a precursor thereof by microbial fermentation.
C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce a product comprising acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C. autoethanogenum permits the conversion of racemic propanediol to acetone and/or propionaldehyde. Entantiomeric starting materials lead to different products. If desired, the products may be reduced to form alcohols. The reaction can be performed in various host cells, so that various materials may be used as carbon and/or energy sources.
A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
2. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.
The invention provides an improved method for the production, separation and recovery of one or more fermentation products from a fermentation broth. Further, the invention provides a method for increasing efficiency of a fermentation reaction. In particular, the invention relates to a fermentation system which incorporates a simulated moving bed for separation of fermentation products from a fermentation broth, and a corresponding method.
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12P 7/08 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques
The invention relates to the production of products such as alcohols and acids by microbial fermentation, particularly microbial fermentation of substrates comprising CO. It more particularly relates to methods and systems for improving efficiency of products by microbial fermentation. In particular embodiments, the invention provides a method of optimizing production of desired products including the step of ascertaining the proportion of CO converted to CO2.
The present invention relates to mutant alcohol dehydrogenase enzymes, microorganisms comprising the mutant alcohol dehydrogenase enzymes, and methods for the production of products by microbial fermentation using the microorganisms.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
41.
Method for controlling the sulphur concentration in a fermentation method
This invention relates generally to methods for increasing the efficiency of microbial growth and production of products, such as alcohols and acids by microbial fermentation of substrates containing carbon monoxide. More particularly the invention relates to the provision of an alternative sulphur source to a liquid nutrient medium, such that sulphur is available to one or more microorganisms of the fermentation.
The invention relates to a process for optimizing the production of ethanol by microbial fermentation, particularly microbial fermentation of substrates comprising CO. The process involves monitoring the hydrogen production by the microorganism, determining an optimum hydrogen production and adjusting the substrate supply rate in response to a change in the hydrogen production thereby keeping the hydrogen production within a desired range.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
43.
Methods and systems for the production of hydrocarbon products
Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.
C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes
C10G 11/18 - Craquage catalytique, en l'absence d'hydrogène, des huiles d'hydrocarbures avec catalyseurs solides mobiles préchauffés selon la technique du "lit fluidisé"
C10K 3/02 - Modification de la composition chimique des gaz combustibles contenant l'oxyde de carbone en vue de produire un carburant amélioré, p. ex. un carburant de pouvoir calorifique différent qui peut ne pas contenir d'oxyde de carbone par traitement catalytique
C12P 5/02 - Préparation des hydrocarbures acycliques
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12M 1/107 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens pour recueillir les gaz de fermentation, p. ex. le méthane
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C01B 3/38 - Production d'hydrogène ou de mélanges gazeux contenant de l'hydrogène par réaction de composés organiques gazeux ou liquides avec des agents gazéifiants, p. ex. de l'eau, du gaz carbonique, de l'air par réaction d'hydrocarbures avec des agents gazéifiants avec des catalyseurs
C10G 9/36 - Craquage thermique non catalytique, en l'absence d'hydrogène, des huiles d'hydrocarbures par contact direct avec des fluides inertes préchauffés, p. ex. avec des métaux ou sels fondus avec des gaz ou vapeurs chauds
44.
Process for production of alcohols by microbial fermentation
The invention provides method for producing butanediol and specifically 2,3-butanediol by anaerobic fermentation of a gaseous substrate comprising CO by a culture of at least one micro-organism. In accordance with particular methods of the invention, 2,3-butanediol is produced by anaerobic fermentation of substrates including carbohydrates and carbon monoxide. The invention further provides for the up-regulation of a native 2,3-butanediol dehydrogenase gene in the at least one micro-organism. The 2,3-butanediol can be further converted to compounds such as butene(s), butadiene and methyl ethyl ketone. These compounds can be further converted to chemical products such as 2-butanol.
The invention provides methods for producing Lactate by anaerobic Fermentation. According to particular methods of the invention, Lactate is produced by anaerobic fermentation of a substrate comprising hydrogen and carbon monoxide.
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
The present invention relates to methods for sustaining a microbial culture during periods of limited substrate supply. In accordance with the methods of the invention, a microbial culture comprising carboxydotrophic bacteria can be sustained during periods of limited substrate supply by maintaining the temperature of the microbial culture at a temperature below an optimum operating temperature. Examples of periods of limited substrate supply include when the microbial culture is transported to a remote location or during time when it is stored.
2 to a primary bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce an acid such as acetate. The acid, e.g. acetate from the primary bioreactor is then provided to a secondary bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
Clostridium autoethanogenum is described which has improved efficiency in the production of ethanol by anaerobic fermentation of substrates comprising carbon monoxide. The bacterium can produce ethanol and acetate at an ethanol to acetate ratio of at least 1.0 and has a productivity of at least 1.2 g of ethanol/l of fermentation broth per day. The bacterium is also characterized in that it has substantially no ability to sporulate.
C12P 7/08 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques
Methods for producing an ester from a gas comprising carbon monoxide. More specifically the invention provides for methods for producing one or more products by the anaerobic fermentation of a gaseous substrate comprising CO and the subsequent conversion of the one or more products to an ester in a down-stream reaction. The invention further provides methods for improving the efficiency of the production of esters from gaseous substrates.
Methods for improving the efficiency of 2,3-butanediol fermentations are disclosed. More specifically methods of increasing the butanediol productivity from the anaerobic fermentation of a substrate comprising carbon monoxide or carbon monoxide and hydrogen by one or more caboxydotrophic acetogenic bacteria are disclosed. The method includes supplying a hydrogen depleted substrate to increase butanediol productivity. The method includes producing butanediol at a volumetric productivity rate of at least 15 g/L/day.
The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.
C12P 7/04 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle acycliques
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
Provided is an inkjet recording apparatus capable of recording a high quality image without limiting the kinds of ink or recording media, including an inkjet head having a plurality of nozzles to eject ink droplets, wherein the ink droplets ejected from the plurality of the nozzles is charged by applying voltage between the inkjet head and the recording medium, and land on the recoding medium so as to record the image on the recording medium, wherein after a first ink droplet lands on the recording medium, a second ink droplet lands on the recording medium so as to overlap with the first ink droplet before discharging of the first ink droplet is completed.
B41J 2/06 - Machines à écrire ou mécanismes d'impression sélective caractérisés par le procédé d'impression ou de marquage pour lequel ils sont conçus caractérisés par la mise en contact sélective d'un liquide ou de particules avec un matériau d'impression à jet d'encre caractérisés par le procédé de formation du jet en produisant à la demande des gouttelettes ou des particules séparées les unes des autres par champ électrique ou magnétique
B41J 2/41 - Machines à écrire ou mécanismes d'impression sélective caractérisés par le procédé d'impression ou de marquage pour lequel ils sont conçus caractérisés par l'alimentation sélective en courant électrique ou l'application sélective d'un champ magnétique à un matériau d'impression ou de transfert d'impression pour l'impression électrostatique
B41J 2/095 - Machines à écrire ou mécanismes d'impression sélective caractérisés par le procédé d'impression ou de marquage pour lequel ils sont conçus caractérisés par la mise en contact sélective d'un liquide ou de particules avec un matériau d'impression à jet d'encre caractérisés par la commande du jet pour une déviation selon plusieurs angles différents du type à commande par champ électrique
B41J 2/21 - Machines à écrire ou mécanismes d'impression sélective caractérisés par le procédé d'impression ou de marquage pour lequel ils sont conçus caractérisés par la mise en contact sélective d'un liquide ou de particules avec un matériau d'impression à jet d'encre pour l'impression à plusieurs couleurs
B41J 11/00 - Dispositifs ou agencements pour supporter ou manipuler un matériau de copie en feuilles ou en bandes
B41J 29/393 - Dispositifs de commande ou d'analyse de l'ensemble de la machine
The present invention relates to improvement in efficiency in gasification for use with syngas fermentation. In particular, the invention relates to increasing the overall carbon capture efficiency of a gasification/fermentation process to produce products such as alcohols.
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
54.
Optimised media containing nickel for fermentation of carbonmonoxide
The invention relates to improvements in the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of substrates comprising CO. It more particularly relates to the provision of an improved fermentation media, comprising nickel, to a fermentation system such that one or more micro-organisms convert a substrate comprising CO to one or more alcohols, such as ethanol. In particular embodiments, a microbial culture is provided with at least 10 μM nickel, such that CO uptake by the microbial culture increases and ethanol productivity improves.
C12P 7/08 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques
A62D 3/02 - Procédés pour rendre les substances chimiques nuisibles inoffensives ou moins nuisibles en effectuant un changement chimique dans les substances par des procédés biologiques, c.-à-d. des procédés utilisant des enzymes ou des micro-organismes
The invention relates to methods for improving the efficiency of carbon capture in microbial fermentation of a gaseous substrate comprising CO and/or H2. In certain aspects the invention relates to improving the efficiency of carbon capture in the microbial fermentation of gaseous substrate comprising CO and/or H2 to produce alcohol(s) and/or acid(s). In particular the invention relates to methods for improving the efficiency of carbon capture in carboxydotrophic fermentation.
The invention relates to the microbial fermentation of gaseous substrates, particularly to gas/liquid contact modules and bioreactors configured to improve the efficiency of fermentations, particularly microbial fermentations of substrates comprising CO. In a particular embodiment, a gas/liquid contact module with multiple channels is configured to produce products in a liquid fermentation broth. In a further particular embodiment, there is provided a method of fermentation of a gaseous substrate to produce a product in a liquid fermentation broth.
The invention relates to the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of substrates comprising CO. It more particularly relates to processes for the production of alcohols from their corresponding acids in the presence of a substrate comprising CO. In particular embodiments, a fermentation reaction producing acid(s) and optionally alcohol(s) is perturbed such that at least a portion one or more of acid(s) is converted to alcohol.
C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas.
A novel class of bacteria is described which has improved efficiency in the production of thanol by anaerobic fermentation of substrates containing carbon monoxide.
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12P 7/08 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques
C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
C12N 1/30 - Procédés utilisant des hydrocarbures ou milieux de culture en contenant aliphatiques ayant au plus cinq atomes de carbone
C12N 1/28 - Procédés utilisant des hydrocarbures ou milieux de culture en contenant aliphatiques
The invention relates to biological processes for producing one or more desired products, including alcohols such as ethanol and butanol. The processes comprise carrying out first and second fermentations of substrates in first and second bioreactors, wherein each fermentation produces one or more desired products and/or one or more by-products that can be utilized in the other fermentation. A product and/or by-product of the first fermentation is introduced to the second bioreactor during the fermentation, and a product and/or by-product of the second fermentation is introduced to the first bioreactor during the fermentation.
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
The invention relates to methods for the production of alcohols by microbial fermentation, particularly microbial fermentation of substrates comprising glycerol and/or acetate, to butanol.
The present invention relates to methods for increasing the efficiency of anaerobic fermentation processes (1) that produce acetate as a by-product in addition to a desired product, and (2) that can utilize hydrogen and/or carbon dioxide in the fermentation. The method comprises the steps of converting acetate produced by the fermentation process into hydrogen gas and carbon dioxide gas, and utilizing hydrogen gas and/or carbon dioxide gas obtained from the acetate conversion in the anaerobic fermentation process. In particular aspects, the invention relates to processes of producing alcohols, particularly ethanol.
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