The present disclosure provides systems, devices, and methods for electroporating at least one cell in a plurality of cells. A cartridge divided into a plurality of separate chambers is provided, where each chamber of the plurality of separate chambers is configured to hold a media comprising the at least one cell. Each chamber of the plurality of separate chambers is fluidly connected via a fluid exchange path, and are functionally separated by constrictions disposed within the fluid exchange path. Each chamber of the plurality of separate chambers includes electrodes for applying a pulsed electrical field to the at least one cell to achieve electroporation and transfection of the cell with nucleic acids. The constrictions disposed between each chamber prevents electric field interference between adjacent chambers during the electroporation procedures.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
The invention relates to a device for providing a cell suspension to an apparatus for treating the cells, wherein the device comprises at least one internal space 16 for holding the suspension, and wherein the internal space 16 is at least partially surrounded by a wall 15. The device further comprises at least one inlet port 2 comprising at least one first opening 3 for introducing the suspension into the internal space 16 and at least one outlet port comprising at least one second opening for removing the suspension from said internal space 16. The inlet port 2 further comprises at least one barrier element 6 bordering the first opening 3 at a side furthest to the wall 15 and is partially arranged between the first opening 3 and the internal space 16.
The invention relates to a method and device for controlling a filling level of a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles within at least one chamber of a device for applying an electric field to the suspension. In order to avoid overfilling of the chamber if multiple electroporation cycles are performed and to achieve exact filling in an environment of unpredictable chamber volume, the amount of suspension filled into the chamber is dynamically limited in the course of several electroporation cycles by determining at least one change of the electrical resistance at the outlet port. The resistance between at least one electrode and a grounding electrode is measured during the filling procedure of each cycle at several points in time. Once a change of resistance is detected, the termination routine is initiated and the filling procedure is finally terminated. Exact filling of the chamber is thus ensured during each electroporation cycle so that enhanced reproductive electroporation performance can be guaranteed.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
G01F 23/24 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p. ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p. ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de résistance de résistances électriques produites par contact avec des fluides conducteurs
G01F 23/80 - Dispositions pour le traitement des signaux
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The invention relates to a device for providing a cell suspension to an apparatus for treating the cells, wherein the device comprises at least one internal space 16 for holding the suspension, and wherein the internal space 16 is at least partially surrounded by a wall 15. The device further comprises at least one inlet port 2 comprising at least one first opening 3 for introducing the suspension into the internal space 16 and at least one outlet port comprising at least one second opening for removing the suspension from said internal space 16. The inlet port 2 further comprises at least one barrier element 6 bordering the first opening 3 at a side furthest to the wall 15 and is partially arranged between the first opening 3 and the internal space 16.
Disclosed is a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. Also disclosed is a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
A61N 1/32 - Application de courants électriques par électrodes de contact courants alternatifs ou intermittents
11.
METHOD AND DEVICE FOR CONTROLLING THE FILLING LEVEL IN A CHAMBER
The invention relates to a method and device for controlling a filling level of a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles within at least one chamber of a device for applying an electric field to the suspension. In order to avoid overfilling of the chamber if multiple electroporation cycles are performed and to achieve exact filling in an environment of unpredictable chamber volume, the amount of suspension filled into the chamber is dynamically limited in the course of several electroporation cycles by determining at least one change of the electrical resistance at the outlet port. The resistance between at least one electrode and a grounding electrode is measured during the filling procedure of each cycle at several points in time. Once a change of resistance is detected, the termination routine is initiated and the filling procedure is finally terminated. Exact filling of the chamber is thus ensured during each electroporation cycle so that enhanced reproductive electroporation performance can be guaranteed.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
G01F 1/56 - Mesure du débit volumétrique ou du débit massique d'un fluide ou d'un matériau solide fluent, dans laquelle le fluide passe à travers un compteur par un écoulement continu en utilisant des effets électriques ou magnétiques
Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
The present invention is directed to methods, compositions and devices useful for the detection and/or quantification of a microbial contaminant, including an endotoxin. In embodiments, cartridges are provided that include dried compositions that are useful in absorbance-based assays and in combination with portable readers/ devices. The cartridges involve a pump mechanism, e.g. a two position syringe, wherein a first position creates a vacuum to introduce the sample into the fluid inlet port and the conduit of the cartridge, and a second position provides transport from the conduit to an optical sample well, which contains a dried hemocyte lysate composition and a dried chromogenic substrate.
G01N 33/579 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir un lysat de limulus
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical and biochemical preparations for industrial and scientific use, in particular for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells; chemical preparations, namely soluble substances and solutions in the nature of buffer and standard solutions, in particular for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells;diagnostic preparations and reagents for scientific or research use; biomedical compounds, namely, peptides, proteins and nucleic acids or complexes of these molecules for scientific, industrial, laboratory or research use, in particular for detecting and monitoring purposes as well as for integration of foreign nucleic acids into the genome of living cells Pharmaceutical and veterinary preparations for treating genetic defects, viral and infectious diseases, cardiovascular diseases, and cancer; diagnostic preparations for medical and veterinary use; living cells and micro-organisms as well as proteins, peptides and nucleic acids or complexes of these molecules for medical and veterinary purposes for treating genetic defects, viral and infectious diseases, cardiovascular diseases, and cancer Scientific apparatus and instruments, namely, electroporators for generating electrical pulses, in particular for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells; scientific apparatus and instruments for electroporation, namely, electroporators for introducing DNA or chromosomes into bacteria or other cells using electricity; apparatus and instruments for conducting, switching, converting, storing, controlling and monitoring of electricity, namely, electroporators for introducing DNA or chromosomes into bacteria or other cells using electricity; devices, containers and reaction vessels for biochemical purposes, in particular for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells, namely, electroporation devices and vessels; test tubes; electrodes made of metal and conductive polymers; laboratory storage tubes, namely, cuvettes made of synthetic material; multi-well plates, including those having electrodes made of metal and conductive polymers, for biochemical purposes, namely, plates having multi-well arrays that can be used in chemical analysis, biological analysis or patterning for scientific, laboratory or medical research use; magnetic recording carriers, namely, prerecorded magnetic data carriers featuring information for use in the field of analysis and control of biochemical processes; prerecorded floppy-disks, compact disks, DVDs and optical data carriers featuring information for use in the field of analysis and control of biochemical processes; data processing devices and computers; peripheral equipment for computers; computer programs and software for use in analyzing and controlling biochemical and biophysical processes in the field of transferring biological molecules into living cells, integrating foreign nucleic acids into the genome of living cells, and transient production of proteins
19.
Method and device for uniformly treating adherent cells
Disclosed is a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. Also disclosed is a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
A61N 1/32 - Application de courants électriques par électrodes de contact courants alternatifs ou intermittents
The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
A61N 1/30 - Appareils d'ionothérapie ou d'électrophorèse
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method,wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
A61N 1/30 - Appareils d'ionothérapie ou d'électrophorèse
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
24.
COMPOSITIONS AND METHODS FOR ENHANCING THE THERAPEUTIC POTENTIAL OF STEM CELLS
The invention encompasses compositions and method of treating a vascular disease such as peripheral artery disease, The methods involve- administering to a patient in need thereof, an effective amount of a composition comprising a population of cells such as mesenchymal stem cells (MSCs) and a non-muscle myosin II antagonist such as blebbistatin. Non-muscle myosin II antagonists are disclosed to surprisingly and dramatically accelerate MSC-triggered regeneration of damaged tissues arid unexpectedly and drastically reduce severe complications of stem cell treatment.
A61K 31/437 - Composés hétérocycliques ayant l'azote comme hétéro-atome d'un cycle, p. ex. guanéthidine ou rifamycines ayant des cycles à six chaînons avec un azote comme seul hétéro-atome d'un cycle condensés en ortho ou en péri avec des systèmes hétérocycliques le système hétérocyclique contenant un cycle à cinq chaînons ayant l'azote comme hétéro-atome du cycle, p. ex. indolizine, bêta-carboline
A61K 35/28 - Moelle osseuseCellules souches hématopoïétiquesCellules souches mésenchymateuses de toutes origines, p. ex. cellules souches dérivées de tissu adipeux
The invention relates to an electrode assembly 20, in particular for applying at least one electric field to adherent cells, comprising at least two electrodes 21, each having at least one surface 32 which is arranged opposite the corresponding surface 32 of the other electrode 21, wherein an electrically insulating material 26 is arranged at least partially between the surfaces 32 of the electrodes 21. The solution according to the invention allows the electric field to be concentrated in the region of the cells to be treated such that a voltage pulse, or the current produced thereby, flows through the cells without the majority flowing away over the cells unused in the electrolyte. The invention further relates to a method for applying at least one electric field to adherent cells, in which the electric field is generated by applying a voltage to at least two electrodes, the electric field is concentrated on the side of the electrodes which faces the cells and/or is limited to the space between the cells and the side of the electrodes which faces the cells.
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
A61N 1/32 - Application de courants électriques par électrodes de contact courants alternatifs ou intermittents
27.
Method and device for uniformly treating adherent cells
The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
A61N 1/32 - Application de courants électriques par électrodes de contact courants alternatifs ou intermittents
The invention relates to a method and device for cultivating attachment-dependent cells in the presence of microcarriers in a flexible cultivation container. In particular, the invention concerns a cultivation method using a flexible, scalable, disposable, substantially gas-permeable and non-liquid-permeable container for expansion of adherent cells on microcarriers. According to the invention, cultivation of the cells is accomplished under orbital shaking at 8 rpm or more than 8 rpm.
C12M 3/02 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des suspensions
29.
METHOD FOR CONTROLLING BINDING OF CELLS TO A SUBSTRATE
The invention relates to a method for promoting the adhesion of cells to a substrate to which these cells usually have no or only low affinity, wherein the adhesion of the cells to the substrate is promoted by supplying the cells with the non-muscle myosin II inhibitor Blebbistatin so as to enable the cells to attach to surfaces to which they otherwise would not have sufficient affinity. Surprisingly, supplying the cells with the inhibitor enhances the capability of these cells to attach to surfaces to which they usually have no or only low affinity, for example, PTFE (Teflon®). The invention further concerns uses of the non- muscle myosin II inhibitor Blebbistatin and devices having at least one surface which is coated with cells that have no or only low affinity to said surface.
The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
31.
Method and device for the electrical treatment of reaction spaces
The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
32.
Method and a device for the electrical treatment of a plurality of containers
The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
34.
METHOD AND ELECTRODE ASSEMBLY FOR TREATING ADHERENT CELLS
The invention relates to an electrode assembly 20, in particular for applying at least one electric field to adherent cells, comprising at least two electrodes 21, each having at least one surface 32 which is arranged opposite the corresponding surface 32 of the other electrode 21, wherein an electrically insulating material 26 is arranged at least partially between the surfaces 32 of the electrodes 21. The solution according to the invention allows the electric field to be concentrated in the region of the cells to be treated such that a voltage pulse, or the current produced thereby, flows through the cells without the majority flowing away over the cells unused in the electrolyte. The invention further relates to a method for applying at least one electric field to adherent cells, in which the electric field is generated by applying a voltage to at least two electrodes, the electric field is concentrated on the side of the electrodes which faces the cells and/or is limited to the space between the cells and the side of the electrodes which faces the cells.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Scientific, photographic, optical, cinematographic,
measuring and controlling apparatus and instruments;
apparatus and instruments for measuring luminescence,
phosphorescence and/or fluorescence; luminometers;
photometers; microscopes; apparatus for recording,
transmission and reproduction of sound and images; magnetic
data carriers; floppy disks, compact disks, DVDs and other
data carriers; data processing devices and computers;
peripheral equipment for computers; computer programs and
software. Scientific and technological services and research as well
as design services relating thereto; scientific research
services for others in the field of biochemistry; industrial
analysis and research services; design and development of
computer hardware and software.
36.
CONTAINER WITH A PLURALITY OF REACTION SPACES AND ELECTRODES
The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n + x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n ≥3 and x is the number of rows, with x ≥ 1.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
37.
A METHOD AND A DEVICE FOR THE ELECTRICAL TREATMENT OF A PLURALITY OF CONTAINERS
The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
38.
METHOD AND DEVICE FOR THE ELECTRICAL TREATMENT OF REACTION SPACES
The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical and biochemical preparations for industrial and
scientific purposes, in particular agents for the transfer
of biological molecules into living cells, integration of
foreign nucleic acids into the genome of living cells and
transient production of proteins; soluble substances and
solutions, in particular buffer and standard solutions for
the transfer of biological molecules into living cells,
integration of foreign nucleic acids into the genome of
living cells and transient production of proteins; aqueous
solutions containing nutrients for cultivating living cells;
biological agents for laboratories, except for medical and
veterinary purposes; diagnostics, except for medical and
veterinary purposes; peptides, proteins and nucleic acids or
complexes of these molecules, in particular for detecting
and monitoring purposes as well as for integration of
foreign nucleic acids into the genome of living cells and
transient production of proteins; expression and control
vectors; reagents for transfection, in particular for
transient production of proteins, except for medical and
veterinary purposes. Pharmaceutical and veterinary preparations, in particular
for medical and clinical purposes for treating genetic
defects in gene therapy, viral and infectious diseases,
cardiovascular diseases and cancer; diagnostic preparations
for medical and veterinary purposes, in particular for
diagnosis and analysis of genetic defects, viral and
infectious diseases, cardiovascular diseases and cancer;
living cells, micro-organisms as well as proteins, peptides
and nucleic acids or complexes of these molecules for
medical and veterinary purposes, in particular for medical
and clinical purposes for treating genetic defects in gene
therapy, viral and infectious diseases, cardiovascular
diseases and cancer. Scientific apparatus and instruments, in particular electric
field and voltage pulse generators for the transfer of
biological molecules into living cells, integration of
foreign nucleic acids into the genome of living cells and
transient production of proteins; apparatus and instruments
for electroporation, in particular electroporators and their
peripheral equipment; apparatus and instruments for
conducting, switching, converting, storing, controlling and
monitoring of electricity, in particular capacitors,
transistors, power semiconductors, relays, transformers,
electrical conductors and contacts, electric measuring and
controlling instruments; laboratory equipment and supplies,
as far as included in this class; devices, containers and
reaction vessels for biochemical purposes, as far as
included in this class, in particular for the transfer of
biological molecules into living cells, integration of
foreign nucleic acids into the genome of living cells and
transient production of proteins; test tubes; electrodes
made of metal and conductive polymers; cuvettes made of
synthetic material; cuvettes, including those having
electrodes made of metal and conductive polymers, for
biochemical purposes; containers and reaction vessels having
several reaction chambers, including those having electrodes
made of metal and conductive polymers, for biochemical
purposes; multi-well plates, including those having
electrodes made of metal and conductive polymers, for
biochemical purposes; magnetic recording carriers;
floppy-disks, compact disks, DVDs and other data carriers;
data processing devices and computers; peripheral equipment
for computers; computer programs and software.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Apparatus and instruments for measuring luminescence, phosphorescence and/or fluorescence, namely, luminometers; Luminometers [ ; Photometers; Microscopes; Computers ] [ Scientific and technological services, namely, biological research and analysis; research and research services in the field of the transfer of biological molecules into living cells, integration of foreign nucleic acids into the genome of living cells and transient expression of proteins; Scientific research services for others in the field of biochemistry; Design and development of computer hardware and software ]
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical and biochemical preparations for industrial and scientific purposes, namely, agents for the transfer of biological molecules into living cells, integration of foreign nucleic acids into the genome of living cells and transient production of proteins; soluble substances and solutions, namely, buffer and standard solutions for industrial and scientific purposes for the transfer of biological molecules into living cells, integration of foreign nucleic acids into the genome of living cells and transient production of proteins; aqueous solutions containing nutrients for cultivating living cells for industrial and scientific purposes; biological agents for laboratories, except for medical and veterinary purposes; diagnostics, except for medical and veterinary purposes; peptides, proteins and nucleic acids or complexes of these molecules for industrial and scientific use for detecting and monitoring purposes as well as for integration of foreign nucleic acids into the genome of living cells and transient production of proteins; chemicals in the nature of expression and control vectors for industrial and scientific use; reagents for transfection for transient production of proteins, except for medical and veterinary purposes Pharmaceutical and veterinary preparations for medical and clinical purposes for treating genetic defects in gene therapy, viral and infectious diseases, cardiovascular diseases and cancer; diagnostic preparations for medical and veterinary purposes for diagnosis and analysis of genetic defects, viral and infectious diseases, cardiovascular diseases and cancer; living cells, micro-organisms as well as proteins, peptides and nucleic acids or complexes of these molecules for medical and clinical purposes for treating genetic defects in gene therapy, viral and infectious diseases, cardiovascular diseases and cancer Scientific apparatus and instruments, namely, electric field and voltage pulse generators for the transfer of biological molecules into living cells, integration of foreign nucleic acids into the genome of living cells and transient production of proteins; apparatus and instruments for electroporation, namely, electroporators and their peripheral equipment; apparatus and instruments for conducting, switching, converting, storing, controlling and monitoring of electricity, namely, capacitors, transistors, power semiconductors, electric relays, transformers, electrical conductors and contacts, and electric measuring and controlling instruments, namely, voltmeters, amperemeters and electrical controllers; devices, containers and reaction vessels being laboratory equipment for biochemical purposes, as far as included in this class, for the transfer of biological molecules into living cells, integration of foreign nucleic acids into the genome of living cells and transient production of proteins, namely, test tubes for laboratory purposes, electrodes made of metal and conductive polymers, cuvettes being laboratory equipment made of synthetic material, cuvettes being laboratory equipment, including those having electrodes made of metal and conductive polymers, for biochemical purposes; containers and reaction vessels being laboratory equipment having several reaction chambers, including those having electrodes made of metal and conductive polymers, for biochemical purposes, and multi-well plates being laboratory equipment, including those having electrodes made of metal and conductive polymers, for biochemical purposes; [ prerecorded magnetic data carriers for use in the field of analysis and control of biochemical processes; prerecorded floppy-disks for use in the field of analysis and control of biochemical processes, prerecorded compact disks for use in the field of analysis and control of biochemical processes, ] prerecorded DVDs for use in the field of analysis and control of biochemical and biophysical processes; data processors and computers; peripheral equipment for computers; computer programs and software, namely, computer software for use in analysing and controlling biochemical and biophysical processes in the field of transferring biological molecules into living cells, integrating foreign nucleic acids into the genome of living cells and transient production of proteins
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical and biochemical preparations for industrial and
scientific use, in particular for the transfer of biological
molecules into living cells and the integration of foreign
nucleic acids into the genome of living cells, chemical
preparations, namely soluble substances and solutions, in
particular for the transfer of biological molecules into
living cells and the integration of foreign nucleic acids
into the genome of living cells; biological agents for
laboratories, except for medical and veterinary purposes;
diagnostics, included in this class; peptides, proteins and
nucleic acids or complexes of these molecules, in particular
for detecting and monitoring purposes as well as for
integration of foreign nucleic acids into the genome of
living cells, included in this class. Pharmaceutical and veterinary preparations; diagnostics,
included in this class; living cells and micro-organisms as
well as proteins, peptides and nucleic acids or complexes of
these molecules for medical and veterinary purposes. Scientific apparatus and instruments, in particular for the
transfer of biological molecules into living cells and the
integration of foreign nucleic acids into the genome of
living cells; apparatus and instruments for electroporation;
apparatus and instruments for conducting, switching,
converting, storing, controlling and monitoring of
electricity; devices, containers and reaction vessels for
biochemical purposes, in particular for the transfer of
biological molecules into living cells and the integration
of foreign nucleic acids into the genome of living cells
included in this class; test tubes; electrodes made of metal
and conductive polymers; cuvettes made of synthetic
material; cuvettes, including those having electrodes made
of metal and conductive polymers, for biochemical purposes
included in this class; containers and reaction vessels
having several reaction chambers, including those having
electrodes made of metal and conductive polymers, for
biochemical purposes; multi-well plates, including those
having electrodes made of metal and conductive polymers, for
biochemical purposes; magnetic recording carriers;
floppy-disks, compact disks, DVDs and other data carriers;
data processing devices and computers; peripheral equipment
for computers; computer programs and software.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical and biochemical preparations for industrial and
scientific use, in particular for the transfer of biological
molecules into living cells and the integration of foreign
nucleic acids into the genome of living cells; soluble
substances and solutions, in particular for the transfer of
biological molecules into living cells and the integration
of foreign nucleic acids into the genome of living cells;
biological agents for laboratories, except for medical and
veterinary purposes; diagnostics, included in this class;
peptides, proteins and nucleic acids or complexes of these
molecules, in particular for detecting and monitoring
purposes as well as for integration of foreign nucleic acids
into the genome of living cells. Pharmaceutical and veterinary preparations; diagnostics,
included in this class; living cells and micro-organisms as
well as proteins, peptides and nucleic acids or complexes of
these molecules for medical and veterinary purposes. Scientific apparatus and instruments, in particular for the
transfer of biological molecules into living cells and the
integration of foreign nucleic acids into the genome of
living cells; apparatus and instruments for electroporation;
apparatus and instruments for conducting, switching,
converting, storing, controlling and monitoring of
electricity; devices, containers and reaction vessels for
biochemical purposes, in particular for the transfer of
biological molecules into living cells and the integration
of foreign nucleic acids into the genome of living cells
included in this class; test tubes; electrodes made of metal
and conductive polymers; cuvettes made of synthetic
material; cuvettes, including those having electrodes made
of metal and conductive polymers, for biochemical purpose
included in this class, containers and reaction vessels
having several reaction chambers, including those having
electrodes made of metal and conductive polymers, for
biochemical purposes; multi-well plates, including those
having electrodes made of metal and conductive polymers, for
biochemical purposes; magnetic recording carriers;
floppy-disks, compact disks, DVDs and other data carriers;
data processing devices and computers; peripheral equipment
for computers; computer programs and software.
44.
Device and method for stabilising the flow through a chamber
A device 1 with at least one electrode 8 for generating an electrical field in a chamber 7 is disclosed. The device 1 comprises at least one input channel 6 for introducing a fluid into the chamber 7, and at least one output channel 11 for discharging the fluid from the chamber 7. Also disclosed is a method for stabilizing the flow of a fluid through a chamber 7 in which an electrical field is generated and which has at least one input channel 6 for introducing the fluid into the chamber and at least one output channel 11 for discharging the fluid from the chamber 8. To avoid undesirable backflow of the fluid due to gas formation, the average inside diameter of the input channel 6 of device 1 is smaller than the average inside diameter of the output channel 11.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Chemical and biochemical preparations for industrial and scientific purposes, namely agents for transferring biological molecules into living cells, integrating foreign nucleic acids into the genome of living cells and transient expression of proteins; soluble substances and solutions for transferring biological molecules into living cells, integrating foreign nucleic acids into the genome of living cells and transient expression of proteins; media for cell culture, namely aqueous solutions containing nutrients for cultivation of living cells; biological agents for scientific research for use in laboratories, other than for medical and veterinary purposes; diagnostics for scientific and research use, other than for medical and veterinary purposes; peptides, proteins and nucleic acids or complexes of these molecules for detecting and monitoring purposes as well as for integrating foreign nucleic acids into the genome of living cells and transient expression of proteins; expression and control vectors; transfection reagents for transient expression of proteins; pharmaceutical and veterinary preparations, namely preparations for treatment of genetic malfunction; diagnostic preparations for medical and veterinary purposes; living cells and micro-organisms as well as proteins, peptides and nucleic acids or complexes of these molecules for clinical or medical laboratory use. (1) Scientific and technological services and research, namely contract research, development of biochemical methods for others, development of electroporation protocols for others, generation of transfected cells for others, production of biologically active molecules for others, and scientific support in the field of chemistry, biochemistry, biology, medicine, medical technology and electrotechnology; industrial analysis and research services in the field of chemistry, biochemistry, biology, medicine, medical technology and electrotechnology; conducting chemical and biochemical analyses; research in the field of chemistry, biochemistry, biology; research in the field of biotechnology, medical technology and electrotechnology; design and development of computer software; development and search services in respect of new products for others.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemical and biochemical preparations for industrial and scientific purposes, in particular for the transfer of biological molecules in living cells, the integration of foreign nucleic acids in the genome of living cells and the transient production of proteins; soluble substances and solutions, in particular for the transfer of biological molecules in cells, the integration of foreign nucleic acids in the genome of living cells and the transient production of proteins; media for cultivating living cells; biological laboratory preparations, other than for medical or veterinary purposes; diagnostic preparations, included in class 1; peptides, proteins and nucleic acids or complexes of these molecules, in particular for detection and control purposes, and for the integration of foreign nucleic acids in the genome of living cells and the transient production of proteins; expression and control vectors; transfection reagents, in particular for the transient production of proteins. Pharmaceutical and veterinary preparations; diagnostic preparations, included in class 5; living cells, microorganisms and proteins, peptides and nucleic acids or complexes of these molecules for medical and veterinary purposes. Scientific and technological services and research; industrial analysis and research services; conducting of chemical and biochemical analyses; research in the field of chemistry, biochemistry, biology and technology; computer software design; research and development for others with regard to new products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
(1) Chemical and biochemical preparations used in industry and science for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells; buffer and standard solutions for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells for scientific and research use; biological agents, namely buffer and standard solutions containing proteins, peptides and/or nucleic acids for laboratory use other than for medical and veterinary purposes; diagnostic preparations other than for medical and veterinary purposes, namely peptides, proteins and nucleic acids or complexes of these molecules, for detecting gene expression and localizing proteins; peptides, proteins and nucleic acids or complexes of these molecules for integrating foreign nucleic acids into the genome of living cells.
(2) Biological agents, namely buffer and standard solutions containing living cells for laboratory use other than for medical and veterinary purposes; pharmaceutical preparations for the treatment of genetic malfunction in gene therapy, viral and infectious diseases, cardiovascular diseases and cancer; diagnostic preparations for medical and veterinary use in the field of genetic malfunction; living cells and microorganisms as well as proteins, peptides and nucleic acids or complexes of these molecules for medical and veterinary purposes.
(3) Scientific apparatus and instruments, namely electrical field generators and capacitor impulse generators for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells; apparatus and instruments for conducting, switching, converting, storing, controlling and monitoring of electricity, namely electrical capacitors, electrical conductors and electric parameter monitor modules, containers and reaction vessels for the transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells, test tubes; electrodes made of metal and conductive polymers; cuvettes made of synthetic material; cuvettes having electrodes made of metal and conductive polymers for biochemical purposes; containers and reaction vessels having several reaction chambers and having electrodes made of metal and conductive polymers for biochemical purposes; multiwall plates having electrodes made of metal and conductive polymers for biochemical purposes; magnetic recording carriers; floppy-disks, compact disks, digital versatile disks (DVDs) and other data carriers containing computer software and/or databases for controlling electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivering of electricity in technologies related to transfer of biological molecules into living cells as well as integration of foreign nucleic acids into the genome of living cells and/or treatment of cells with electric current; data processing devices, computers and peripheral equipment for controlling electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivering of electricity in technologies related to transfer of biological molecules into living cells as well as integration of foreign nucleic acids into the genome of living cells and/or treatment of cells with electric current; computer programs and software for controlling electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivering of electricity in technologies related to transfer of biological molecules into living cells and the integration of foreign nucleic acids into the genome of living cells.
(4) Electroporators.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical preparations for industrial and scientific
purposes. Pharmaceutical and veterinary preparations as well as
sanitary preparations, aforementioned goods only for use in
the field of gene therapy; dietetic preparations for medical
use in the field of gene therapy. Scientific apparatus and instruments, namely devices for the
transfer of molecules and devices for detection, selection
and/or sorting of cells into which the molecules are
transferred.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemicals and biochemicals used in industry and science for
transferring biological molecules into living cells and
integrating foreign nucleic acids into the genome of living
cells; soluble substances and solutions for transferring
biological molecules into living cells and integrating
foreign nucleic acids into the genome of living cells;
biological agents for laboratory use (other than for medical
and veterinary purposes); diagnostics (other than for
medical and veterinary purposes); peptides, proteins and
nucleic acids or complexes of these molecules for detecting
gene expression and localizing proteins as well as for
integrating foreign nucleic acids into the genome of living
cells. Pharmaceutical and veterinary preparations; diagnostics for
medical and veterinary purposes; living cells and
micro-organisms as well as proteins, peptides and nucleic
acids or complexes of these molecules for medical and
veterinary purposes. Scientific apparatus and instruments for transferring
biological molecules into living cells and integrating
foreign nucleic acids into the genome of living cells;
apparatus and instruments for electroporation; apparatus and
instruments for conducting, switching, converting, storing,
controlling and monitoring of electricity; devices,
containers and reaction vessels used in biochemistry for
transferring biological molecules into living cells and
integrating foreign nucleic acids into the genome of living
cells; test tubes; electrodes made of metal and conductive
polymers; cuvettes made of synthetic material; cuvettes
having electrodes made of metal and conductive polymers for
biochemical purposes; containers and reaction vessels having
several reaction chambers including electrodes made of metal
and conductive polymers for biochemical purposes; muli-well
plates having electrodes made of metal and conductive
polymers for biochemical purposes; magnetic recording
carriers; floppy-disks, compact disks, digital versatile
disks (DVDs) and other data carriers; data processing
devices and computers; peripheral equipment; computer
programs and software.
50.
Method for treating small volumes with electrical current
Disclosed is a method for treating biological material via electrical current, in which the biological material is added to a small volume of a buffer solution having relative high ionic strength. A strong electrical field is generated in the buffer solution by a high voltage pulse having a preset duration.
The biological material is added to at most 50 μl of a buffer solution with an ionic strength of at least 100 mmol/l. By at least one voltage pulse having a preset duration of at least 10 μs, an electrical field with a field strength of at least 1 kV/cm is generated in the buffer solution. The voltage pulse is hereby interrupted at least once for a duration of at least 100 μs and is then again continued.
09 - Appareils et instruments scientifiques et électriques
17 - Produits en caoutchouc ou en matières plastiques; matières à calfeutrer et à isoler
20 - Meubles et produits décoratifs
Produits et services
Scientific apparatus and instruments; biochemical apparatus
and instruments; apparatus and instruments for conducting of
electricity; electric conductors; electrodes made of metal
or conductive polymers; devices, containers and reaction
vessels for biochemical purposes; devices, containers and
reaction vessels for transferring biological molecules into
living cells; disposables for laboratories, namely
containers, reaction vessels, test tubes, multiwell plates
and cuvettes made of synthetic material; test tubes;
cuvettes made of synthetic material; cuvettes, including
those having electrodes made of metal or conductive
polymers, for biochemical purposes; containers and reaction
vessels having several reaction chambers, including those
having electrodes made of metal or conductive polymers, for
biochemical purposes; multiwell plates, including those
having electrodes made of metal or conductive polymers, for
biochemical purposes. Goods made of synthetic material (sub-assemblies); synthetic
material, partly processed; isolators for electric
conductors. Containers, except those made of metal or masonry;
containers made of plastic; closures and covers for
containers, except those made of metal; frames and holders
for containers, except those made of metal; frames and
holders for reaction vessels, cuvettes and multiwell plates,
except those made of metal; sealing caps, except those made
of metal; packing containers made of plastic.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
(1) Chemicals and biochemicals used in industry and science for transferring biological molecules into living cells and integrating foreign nucleic acids into the genome of living cells; soluble substances and solutions for transferring biological molecules into living cells and integrating foreign nucleic acids into the genome of living cells.
(2) Biological agents for laboratory use (other than for medical and veterinary purposes); diagnostic preparations for scientific and research use in technologies related to transfer of biological molecules into living cells and/or integration of foreign nucleic acids into the genome of living cells (other than for medical and veterinary purposes); peptides, proteins and nucleic acids or complexes of these molecules for detecting gene expression and localizing proteins as well as for integrating foreign nucleic acids into the genome of living cells; pharmaceutical and veterinary preparations, namely preparations for treatment of genetic malfunction; diagnostics preparations for medical and veterinary use in the field of genetic malfunction; living cells and micro-organisms as well as proteins, peptides and nucleic acids or complexes of these molecules for medical and veterinary purposes.
(3) Reaction vessels used in biochemistry for transferring biological molecules into living cells and integrating foreign nucleic acids into the genome of living cells, namely cuvettes and multiwell plates to be filled with probes containing biological molecules; test tubes; electrodes made of metal and conductive polymers; cuvettes made of synthetic material; cuvettes having electrodes made of metal and conductive polymers for biochemical purposes; reaction vessels having several reaction chambers including electrodes made of metal and conductive polymers for biochemical purposes; multi-well plates having electrodes made of metal and conductive polymers for biochemical purposes; magnetic recording carriers, floppy-disks, compact disks, digital versatile disks (DVDs) and other data carriers containing computer software and/or databases for controlling electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivering of electricity in technologies related to transfer of biological molecules into living cells as well as integration of foreign nucleic acids into the genome of living cells and/or treatment of cells with electric current; data processing devices, computers and peripheral equipment for controlling electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivery of electricity in technologies related to transfer of biological molecules into living cells as well as integration of foreign nucleic acids into the genome of living cells and/or treatment of cells with electric current; computer programs and software for controlling electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivery of electricity in technologies related to transfer of biological molecules into living cells as well as integration of foreign nucleic acids into the genome of living cells and/or treatment of cells with electric current.
(4) Apparatus and instruments for electroporation, namely electroporators, electrical field generators and capacitor impulse generators for laboratory use in conducting, switching, converting, storing, controlling, guiding and delivery of electricity in technologies related to transfer of biological molecules into living cells as well as integration of foreign nucleic acids into the genome of living cells and/or treatment of cells with electric current.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Chemicals and biochemicals used in industry and science for transferring biological molecules into living cells and integrating foreign nucleic acids into the genome of living cells; buffer and standard solutions for transferring biological molecules into living cells and integrating foreign nucleic acids into the genome of living cells for scientific or research use; biological agents, namely, buffer and standard solutions containing living cells, proteins, peptides and/or nucleic acids for laboratory use other than for medical and veterinary purposes; diagnostic preparations other than for medical and veterinary purposes; Biochemical reagents for laboratories, namely, peptides, proteins and nucleic acids or complexes of these molecules for detecting gene expression and localizing proteins as well as for integrating foreign nucleic acids into the genome of living cells Pharmaceutical preparations, namely, preparations for treatment of genetic malfunction in gene therapy; veterinary preparations, namely, preparations for treatment of genetic malfunction in gene therapy; diagnostic preparations for medical and veterinary purposes; microorganisms and biological material for medical and veterinary purposes, namely, living cells as well as proteins, peptides and nucleic acids or complexes of these molecules
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical and biological preparations for industrial and
scientific purposes; soluble substances and solutions, in
particular for the transfer of biological molecules into
cells; biological agents for laboratories, except for
medical and veterinary use; diagnostics, as far as included
in this class; proteins and nucleic acids for detection
purposes, in particular as positive control. Pharmaceutical and veterinary preparations; diagnostics, as
far as included in this class; microorganisms, proteins,
peptides, nucleic acids and other biological material for
medical and veterinary purposes. Scientific apparatus and instruments; biochemical apparatus
and instruments; measuring apparatus and instruments;
control apparatus and instruments; apparatus and instruments
for conducting, switching, converting, storing, controlling
and monitoring of electricity; electrical conductors;
electrodes made of metal and conductive polymers; devices,
containers and reaction vessels for biochemical purposes;
devices, containers and reaction vessels for the transfer of
biological molecules into living cells; disposables for
laboratories; test tubes; cuvettes made of synthetic
material; cuvettes, including those having electrodes made
of metal and conductive polymers, for biochemical purposes;
containers and reaction vessels having several reaction
chambers, including those having electrodes made of metal
and conductive polymers, for biochemical purposes;
multi-well plates, including those having electrodes made of
metal and conductive polymers, for biochemical purposes;
magnetic recording medium; floppy disks, compact disks, DVDs
and other data carriers; data processing units and
computers; computer peripheral devices; computer programs
and computer software.
55.
Device and method for processing biological material
Disclosed is a device for processing biological material which at least comprises a chamber at least closable to the outside and having an inner space for receiving the biological material. The chamber comprises at least one electrode placed in contact with the inner space for generating an electric field. Also disclosed is a method for processing biological material. The biological material is introduced into the inner space above and the electrode can generate an electric field after said biological material is introduced by applying voltage to said electrode and a further electrode in contact with the inner space. The chamber comprises at least one inlet line having at least one opening arranged close to the electrode. The biological material is almost completely rinsed out of the inner space after the electric field is generated, via a solution guided via an inlet line of the chamber along at least one electrode.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation
56.
Method and circuit arrangement for treating biomaterial
The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse. The invention also relates to a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. The present invention provides a controller for monitoring the chronological progression of the voltage pulse. This controller controls at least one continuation of discharge after termination.
The invention concerns a container 1 with chambers 2 which each comprise at least one pair of electrodes including a first 4 and a second electrode 5 for the application of electric voltage for generating an electric field within one chamber 2. At least two first electrodes 4 of different chambers 3 are conductively coupled and at least one second electrode 5 of said chamber 2 is separately conductively connectable. The invention further concerns a method for manufacturing said container 1 as well as a device for electrically contacting at least one of said containers 1.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 13/00 - Traitement de micro-organismes ou d'enzymes par énergie électrique ou ondulatoire, p. ex. par magnétisme, par des ondes sonores
09 - Appareils et instruments scientifiques et électriques
17 - Produits en caoutchouc ou en matières plastiques; matières à calfeutrer et à isoler
20 - Meubles et produits décoratifs
Produits et services
Scientific apparatus and instruments; biochemical apparatus and instruments; apparatus and instruments for conducting electricity; electrical conductors; electrodes of metal and conductive polymers; equipment, containers and reaction vessels for biochemical purposes; equipment, containers and reaction vessels for the transfer of biological molecules in living cells; disposable articles for laboratories; test tubes; cuvettes of plastic; cuvettes, including with electrodes of metal and conductive polymers, for biochemical purposes; containers and reaction vessels with multiple reaction chambers, including with electrodes of metal and conductive polymers, for biochemical purposes; microtitre plates, including with electrodes of metal and conductive polymers, for biochemical purposes. Plastics in extruded form for use in manufacture; semi-processed plastic substances; insulators for electric conductors. Tanks, not of metal or masonry; containers of plastic; container closures and coverings, not of metal; sealing caps, not of metal; frames and holders for containers, not of metal; frames and holders for reaction vessels, cuvettes and microtitre plates, not of metal; packaging containers of plastic.
09 - Appareils et instruments scientifiques et électriques
20 - Meubles et produits décoratifs
Produits et services
Laboratory equipment and supplies, namely specimen containers, reaction vessels, reaction vessels with several reaction chambers, test tubes, cuvettes, and multiwell plates, including those having electrodes made of metal or conductive polymers, for transferring biological molecules into living cells Containers, not of metal for commercial use; non-metal and non-paper closures for containers; plastic wall frames for holding containers, reaction vessels, cuvettes, and multiwell plates; packing containers of plastic material
60.
Method for generating an elecrically contactable area on a doped polymer and formed body produced by this method
The invention relates to a method for generating at least one electrically contactable area on a polymer which is doped with a conductive substance, wherein a contact material is applied onto the polymer, which has a lower specific resistance at 23° C. than the polymer. According to the invention the contact material is applied onto the polymer so tightly that close contact between the contact material and the conductive substance is achieved. Due to the tight application of the contact material, which has a lower specific resistance than the polymer, the input resistance of the doped polymer is effectively reduced. The invention further concerns a formed body made of a polymer which is doped with a conductive substance, which has at least one contactable area, within which a contact material is applied onto the polymer, which has a lower specific resistance at 23° C. than the polymer. According to the invention the contact material is applied onto the polymer so tightly that it is in close contact to the conductive substance. Such formed body has a significantly reduced input resistance.
B32B 15/08 - Produits stratifiés composés essentiellement de métal comprenant un métal comme seul composant ou comme composant principal d'une couche adjacente à une autre couche d'une substance spécifique de résine synthétique
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
H01C 1/142 - Bornes ou points de prise spécialement adaptés aux résistancesDispositions de bornes ou points de prise sur les résistances les bornes ou points de prise étant constitués par un revêtement appliqué sur l'élément résistif
A61N 1/30 - Appareils d'ionothérapie ou d'électrophorèse
A61N 1/32 - Application de courants électriques par électrodes de contact courants alternatifs ou intermittents
Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryotic cells using electrical current
The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
F41B 15/04 - BâtonsMatraquesCannesGourdins avec moyens électriques pour étourdir
H01T 23/00 - Appareils pour la production d'ions destinés à être introduits dans des gaz à l'état libre, p. ex. dans l'atmosphère
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Kits comprising containers, vials, and/or applicators containing solutions, cells and/or biological material for laboratory use in technologies related to gene therapy and the transfer of bioactive molecules into cells.
(2) Scientific, electric apparatus and instruments, namely, electrical field generators and capacitor impulse generators for laboratory use in technologies related to gene therapy and transfer of bioactive molecules into cells; fluorescence detectors for use in detection of cells transfected with bioactive molecules; cuvettes for use in the transfer of bioactive molecules into cells.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific, electric apparatus and instruments, in particular apparatus for the transfection of cells or cell nuclei with nucleic acids and/or other molecules.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Chemicals used in industry and science.
(2) Pharmaceutical and veterinary preparations for the treatment of gene-based diseases, for use in the field of diagnostics dependent upon the delivery of said pharmaceuticals to a target site, and for use in the field of gene therapy. (1) Industrial and scientific research services.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Chemicals used in industry and science and pharmaceutical, veterinary and sanitary preparation, dietetic substances adapted for medical use, namely, chemicals and biochemical products, namely, reagents for scientific or medical research use for use in the transfection of bioactive molecules into cells, and for use in the direct or indirect detection of said molecules in association with cells.
(2) Pharmaceutical and veterinary preparations for the treatment of gene-based diseases, such as cancer, cardiovascular disorders and malfunctions of the central nerve system, and for the use in the filed of diagnostics dependent on the delivery of said pharmaceuticals to a target site, and for use in the field of gene therapy. (1) Industrial and scientific research services.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Chemicals used in industry and science. Pharmaceutical and veterinary preparations and sanitary preparations, all the aforesaid goods only in the field of gene therapy; dietetic substances adapted for medical use within the framework of gene therapy.
