Abrégé

The present invention provides a mouse anti-OXA-48 carbapenemase hybridoma cell strain, a monoclonal antibody and use. An Ig variable region gene is cloned by means of mouse hybridoma monoclonal antibody screening and RT-PCR to obtain a hybridoma cell strain capable of stably secreting a mouse anti-OXA-48 carbapenemase antibody and a variable region sequence thereof; a systematic evaluation shows that the mouse anti-OXA-48 carbapenemase antibody has good performance in various aspects, with the titer reaching 1:1280000 or above, and is suitable for use as an immunodiagnostic reagent for preparing a carbapenemase drug-resistant strain in-vitro diagnostic kit.

Classes IPC  ?

• C07K 16/40 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des enzymes
• C12N 15/13 - Immunoglobulines
• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux

Abrégé

The present invention provides a mouse anti-MCR-1/MCR-2 protein hybridoma cell strain, a monoclonal antibody and use. An Ig variable region gene is cloned by means of mouse hybridoma monoclonal antibody screening and RT-PCR to obtain a hybridoma cell strain capable of stably secreting a mouse anti-MCR-1/MCR-2 protein antibody and a variable region sequence thereof; a systematic evaluation shows that the mouse anti-MCR-1/MCR-2 protein antibody has good performance in various aspects, with the titer reaching 1:1280000 or above, and is suitable for use as an immunodiagnostic reagent for preparing a polymyxin drug-resistant strain in-vitro diagnostic kit.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
• C12N 15/13 - Immunoglobulines
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

Provided are a mouse anti-NDM carbapenemase hybridoma cell strain, a monoclonal antibody and use. An Ig variable region gene is cloned by means of mouse hybridoma monoclonal antibody screening and RT-PCR to obtain a hybridoma cell strain capable of stably secreting a mouse anti-NDM carbapenemase antibody and a variable region sequence thereof; a systematic evaluation shows that the mouse anti-NDM carbapenemase antibody has good performance in various aspects, with the titer reaching 1:1280000 or above, and is suitable for use as an immunodiagnostic reagent for preparing a carbapenemase drug-resistant strain in-vitro diagnostic kit.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C07K 16/40 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des enzymes
• C12N 15/13 - Immunoglobulines
• G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes

Abrégé

The present invention provides a mouse anti-MCR-1 protein hybridoma cell strain, a monoclonal antibody produced thereby, and an application. The mouse anti-MCR-1 protein antibody is suitable for being used as an immunodiagnostic reagent for in vitro diagnosis of an MCR-1 protein antigen.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C12N 15/13 - Immunoglobulines
• C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
• G01N 33/74 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des hormones
• G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux

Abrégé

The present invention provides an anti-VIM carbapenemase hybridoma cell strain, a monoclonal antibody secreted thereby, and an application. The anti-VIM carbapenemase monoclonal antibody is suitable for in vitro diagnosis of VIM carbapenemase, and a prepared kit or microfluidic chip can be used for early typing of drug-resistant strains, guiding medication, and assisting clinical infection control and treatment.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C12N 15/13 - Immunoglobulines
• C07K 16/40 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des enzymes
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
• G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes

Abrégé

The present invention provides an anti-KPC type carbapenemase hybridoma cell line, a monoclonal antibody produced therefrom, and an application thereof. The anti-KPC-type carbapenemase monoclonal antibody is suitable for use as an immunodiagnostic reagent for in vitro diagnosis of KPC-type carbapenemase, and a prepared and obtained kit or microfluidic chip can be used for early typing of drug-resistant strains, guiding medication, and assisting clinical infection control and treatment.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C07K 16/40 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des enzymes
• C12N 15/13 - Immunoglobulines
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes

Abrégé

Provided is an anti-IMP type carbapenemase hybridoma cell strain. The hybridoma cell strain capable of stably secreting an anti-IMP type carbapenemase antibody and a variable region sequence thereof are obtained by means of screening a mouse hybridoma monoclonal antibody and cloning an Ig variable region gene by means of an RT-PCR method, and the antibody binding specificity is identified by means of an ELISA method; the antibody can be used for detecting IMP type carbapenemase, the titer reaches 1:640000 or more, and same can be prepared into an in-vitro diagnostic kit or microfluidic chip for early typing of drug-resistant strains, guiding medication and assisting clinical infection control and treatment.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C07K 16/40 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des enzymes
• C12N 15/13 - Immunoglobulines
• G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux

Abrégé

Provided is an anti-OXA-23 type carbapenase hybridoma cell line. An Ig variable region gene is cloned by mouse hybridoma monoclonal antibody screening and an RT-PCR method, a hybridoma cell line capable of stably secreting an anti-OXA-23 type carbapenase antibody and a variable region sequence thereof are obtained, and the antibody binding specificity is identified by means of ELISA. The antibody can be used for detecting an OXA-23 type carbapenase, the titer reaches 1:640000 or above, the antibody can be prepared into an in-vitro diagnostic kit or a microfluidic chip, and the antibody can be used for early typing of drug-resistant strains, medication guide, and assistance in clinical infection control and treatment.

Classes IPC  ?

• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• C07K 16/40 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des enzymes
• G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes

Produits et services

Medicines for human purposes; chemico-pharmaceutical preparations; drugs for medical purposes; serums; nutritive substances for microorganisms; dietetic substances adapted for medical use; depuratives; adhesive tapes, for medical purposes; dental abrasives. Medical apparatus and instruments; analytical apparatus for medical purposes; diagnostic apparatus for medical purposes; dental apparatus, electric; roentgen apparatus for medical purposes; physiotherapy apparatus; beds, specially made for medical purposes; surgical implants (artificial materials); suture materials.