Abrégé

A low-frequency epigenetic marking method for aiding diagnosis of hereditary deafness, which method belongs to the field of hereditary deafness detection. The method comprises: firstly, extracting genomic DNA of a sample to be tested; then, performing amplification under the action of hot-start DNA polymerase by using allele-specific primer sets corresponding to all mutation sites to be tested and by taking the DNA of the sample to be tested as a template; hybridizing the amplification product with a chip, and washing and drying the hybridized chip; and finally, scanning the chip, and analyzing and interpreting the scan results. The method can realize the detection of multiple hereditary deafness genes and mutation sites, fully eliminates interference factors in the detection process, has a high detection speed and high accuracy, and improves the diagnostic quality of hereditary deafness.

Classes IPC  ?

• C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
• C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes

Abrégé

A β-thalassemia related gene mutation detection kit, relating to the field of kits. The β-thalassemia related gene mutation detection kit comprises a kit body (1); the top of the kit body (1) is connected to a kit cover (2) by means of a pin shaft; a clamping mechanism (10) is provided inside the kit body (1). The effect of simultaneously clamping reagent bottles having different sizes within a certain range can be achieved, the application range of the kit is widened, and the reagent bottles inside the kit can be protected in a partitioned mode, thereby preventing fragments from damaging a user or other unbroken reagent bottles after one reagent bottle is broken, solving the problem of leakage of a detection liquid caused because a reagent bottle for storing a β-thalassemia related gene mutation detection liquid is easily broken accidentally during shaking of the kit body (1), and greatly improving the stability of the β-thalassemia related gene mutation detection liquid in the reagent bottle.

Classes IPC  ?

• B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai

Abrégé

A methylmalonic acidemia-related gene mutation detection kit, belonging to the field of kits, and comprising a kit body (1). A U-shaped slot is formed in the inner wall of the kit body (1) close to the top; a kit cover (2) is slidably connected in the U-shaped slot; a rectangular frame (9) is installed in the kit body; a bottom plate (3) is engaged with the inner wall of the rectangular frame (9) close to the bottom; a limiting plate (11) is limitedly slidably connected to the inner wall of the rectangular frame (9) close to the top; a plurality of first sockets (12) penetrate through and are connected to the limiting plate (11). The present invention may implement free adjustment and position limitation according to different lengths of reagent bottles, individually protects each reagent bottle so as to prevent a liquid from contaminating the kit body after a reagent bottle is broken, and achieves the effect that the breakage and liquid leakage of a reagent bottle can be quickly discovered by a worker, solving the problem that when a reagent bottle storing a methylmalonic acidemia-related gene mutation detection liquid is accidentally broken, a detection liquid flows out and enters the kit to contaminate detection liquids in other reagent bottles.

Classes IPC  ?

• B65D 25/10 - Dispositifs pour placer les objets dans les réceptacles

Abrégé

A test kit for spinal muscular atrophy related gene mutation, which relates to the technical field of test kits. The test kit comprises a DNA polymerase, a PCR buffer, 25 mmol/L MgCl2, 10 mmol/L dNTPs, genomic DNA of normal genotype samples, and a test kit body. The test kit body comprises a kit body (1) and a kit cover (2), and test tubes (5) can be held effectively and firmly by means of an additionally provided placement plate (3). The test tubes (5) are sequentially placed through annular conversion sleeves (4) such that the test tubes are protected and fixed by the soft annular conversion sleeves (4), and then the kit cover (2) and the kit body (1) are fitted together to make a pair of electrifying pieces (9) come into contact, so as to electrify the annular conversion sleeves (4) and cause hardening of an electrorheological fluid under the action of current, such that the test tubes can be further fixed by the hard annular conversion sleeves (4) and are fixed more firmly. The friction and isolation of friction strips can not only allow the test tubes to be fixed more firmly, but also effectively prevent the test tubes from being damaged by force.

Classes IPC  ?

• B65D 25/02 - Garnitures internes

Abrégé

A phenylketonuria-related gene mutation test kit, comprising a kit body (1) and a kit cover (2), wherein a holding platform (3) is fixedly mounted in the kit body (1), and a plurality of test tube holders are provided at an upper end of the holding platform (3). A pressing bag (9) and an exhaust bag (4) are additionally provided to exhaust air in the sealed kit. By means of applying force to the pressing bag (9) for squeezing, the exhaust bag (4) is inflated and expanded to fill up empty space in the kit and squeeze out the air; and at the same time, a blocking block (7) is used to block an exhaust hole (6) to prevent air from entering from the exhaust hole (6).

Classes IPC  ?

• B65D 25/02 - Garnitures internes
• B65D 53/00 - Éléments d'étanchéité ou d'emballageÉtanchéité réalisée par matériaux liquides ou plastiques

Abrégé

A kit facilitating querying of reagent tube information, comprising a kit body (1) and a fixed plate (2). A box cover is hingedly connected to the kit body; the fixed plate is fixedly connected to an inner end of the kit body; a movable plate (3) is mounted at the inner end of the kit body; a plurality of coaxially arranged placement holes (21) and a plurality of coaxially arranged placement slots (31) are formed in the fixed plate and the movable plate; reagent tubes (4) are slidably connected in the placement holes and the placement slots; in-tube article information stickers are attached to outer walls of the reagent tubes; a pair of symmetrically distributed rectangular slots (22) are formed in the fixed plate; rectangular plates (5) are fixedly connected to the left and right ends of the movable plate. The kit can enable all the reagent tubes to move upwards synchronously, and the stickers attached with related information of the interior of the reagent tubes are exposed, so as to facilitate information verification by personnel, thereby saving time and labor in the querying process.

Classes IPC  ?

• B65D 25/10 - Dispositifs pour placer les objets dans les réceptacles
• B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

A kit facilitating storing an elongated reagent bottle, the kit comprising a kit body (1), wherein a top storage plate adjusting assembly (2) is arranged in the kit body and comprises an inner groove (21), a screw rod (22) is rotatably connected between the top and the bottom of an inner wall of the inner groove by means of a bearing, a threaded block (23) is screwed to an outer surface of the screw rod, a top storage member (24) is fixedly connected to one side of the threaded block and comprises a top storage plate (241), a first circular placement groove (242) is provided in the top storage plate, a second circular placement groove (243) is provided in the top storage plate and at the bottom of the first circular placement groove, and a third circular placement groove (244) is provided in the top storage plate and at the bottom of the second circular placement groove. By means of the kit, the elongated reagent bottle can be stored in the kit, and the elongated reagent bottle without the need to be stored separately.

Classes IPC  ?

• B65D 25/24 - Accessoires externes pour espacer l'assise du réceptacle de la surface portante, p. ex. pieds
• B65D 25/02 - Garnitures internes
• B65D 25/10 - Dispositifs pour placer les objets dans les réceptacles
• B65D 85/30 - Réceptacles, éléments d'emballage ou paquets spécialement adaptés à des objets ou à des matériaux particuliers pour objets particulièrement sensibles aux dommages par chocs ou compression

Abrégé

Disclosed in the present invention is a detection kit based on gene mutation related to recessive hereditary deafness, which relates to the technical field of kits. A reagent can be conveniently used in a special sealing mode of detachable seal between a sealing cover and a reagent tube, during detecting and diagnosing, a detection rod corresponding to a reagent is pressed down by opening a detection top cover, then an air overflow ball in a corresponding sealing cover is pressed, the air overflow ball is broken to cause a chemical reaction, a large amount of gas is released, and the gas safely expands in the reagent tube by means of a detection assembly, such that inert gas except the liquid reagent in the reagent tube is pressed to detect a leakage, then the purity of the reagent in the reagent tube is indirectly inferred, the diagnosis accuracy can be guaranteed during detection, and the detection convenience of the reagent kit is improved.

Classes IPC  ?

• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12M 1/10 - Appareillage pour l'enzymologie ou la microbiologie montés rotativement
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
• C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

A diagnostic kit having a storage effect, comprising a kit body (1) and a matched kit cover (2). An enclosing strip (3) is adhered on the outer side of a connection position of the kit body (1) and the kit cover (2); inner sealing strips (5) are fixedly connected to the ends of the inner sides of the kit body (1) and the kit cover (2) close to each other, and a pair of the inner sealing strips (5) abut against each other; a plurality of uniformly distributed isolation plates (9) are connected to the inner sealing strips (5) in an embedded manner; a plurality of uniformly distributed adhesive storage grooves are formed in the ends of the inner sealing strips (5) close to each other, a water-soluble insertion strip (7) is fixedly connected at an opening in the outer side of each adhesive storage groove, an adhesive blocking film layer (8) is fixedly connected to the inner side of the adhesive storage groove, and a liquid waterproof adhesive is filled between the adhesive blocking film layer (8) and the adhesive storage groove; a plurality of uniformly distributed migration holes are formed in the upper end of each inner sealing strip (5), and the migration holes are communicated with the adhesive storage grooves; matched magnetic pressing blocks (6) are slidably connected into the migration holes, and the magnetic pressing blocks (6) extend into the adhesive storage grooves and are fixedly connected to the adhesive blocking film layers (8). According to the kit, an external moisture-proof function can be achieved, and a second sealing procedure can be triggered after water invasion, so that a storage effect of the kit in a high-humidity environment is remarkably improved.

Classes IPC  ?

• B65D 81/26 - Adaptations pour empêcher la détérioration ou l'altération du contenuApplications au réceptacle ou au matériau d'emballage d'agents de conservation des aliments, de fongicides, d'insecticides ou de produits repoussant les animaux avec dispositifs pour évacuer ou absorber les fluides, p. ex. s'écoulant du contenuEmploi de produits empêchant la corrosion ou de dessiccateurs
• B65D 25/02 - Garnitures internes
• B65D 53/00 - Éléments d'étanchéité ou d'emballageÉtanchéité réalisée par matériaux liquides ou plastiques

Abrégé

A tumor cell auxiliary diagnosis kit. In the kit, a reagent is easy to access through a threaded seal between a sealing plug (7) and a test tube (8), while the sealing performance is sensed by means of a self-heating sensing capsule (9) in the sealing plug (7). When the sealing fails, outside air first enters the test tube (8), and the self-heating sensing capsule (9) in the sealing plug (7) can sense the oxygen in the air in a timely manner and react with the oxygen to generate heat, causing the self-heating sensing capsule (9) to partially melt and then expanding downward under the action of magnetic attraction, so as to provide early warning of sealing failure to technicians by means of visual changes to avoid misuse of the contaminated reagent, thereby improving the reliability and safety of reagent diagnosis.

Classes IPC  ?

• B65D 25/02 - Garnitures internes
• B65D 25/10 - Dispositifs pour placer les objets dans les réceptacles
• B65D 51/24 - Fermetures non prévues ailleurs combinées avec dispositifs auxiliaires pour des buts autres que la fermeture
• B65D 53/02 - Colliers ou bagues
• G08B 5/00 - Systèmes de signalisation optique, p. ex. systèmes d'appel de personnes, indication à distance de l'occupation de sièges

Abrégé

A dried blood spot-based trace DNA extraction method, comprising: preparing a high-efficiency liquid-phase separation tube; collecting a DNA solution and then mixing same with 100 ng of a carrier RNA; adding an equal volume of phenol chloroform, and transferring same into the high-efficiency liquid-phase separation tube; performing high-speed centrifugation to perform liquid-phase stratification; and recovering 98% of a supernatant.

Classes IPC  ?

• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN

Abrégé

Provided by the present invention is a method for optimizing a trace cfDNA extraction joined high-throughput sequencing library, which is characterized in: after collecting a solution, adding an equal volume of phenol chloroform, then adding 100 ng of carrier DNA and mixing same, preparing a highly efficient liquid-phase separation tube, transferring a digested liquid into the highly efficient liquid-phase separation tube, carrying out high-speed centrifugation for liquid-phase layering, and more than 98% of the amount of the DNA solution can be recycled. The method according to the present invention has the characteristics such as a larger library amount may be achieved, the operation is simple.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques