Abrégé

The present invention refers to a compound which is a pyrazolyl compound for use as a medicament in a neurodegenerative disease or disorder, preferably reactive gliosis. Also, a pyrazolyl compound that is 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1) for use as a medicament in a disease or disorder, preferably for the treatment, reversal or the attenuation of reactive gliosis, and/or reactive gliosis directly or indirectly associated with an eye disease, disease of the retina, or retinal disorder.. Further, the invention discloses pharmaceutical compositions thereof and a method for treating a neurodegenerative disorder, preferably reactive gliosis, comprising administering to a subject in need thereof an effective amount of such compound or pharmaceutical composition.

Classes IPC  ?

• A61K 31/416 - 1,2-Diazoles condensés avec des systèmes carbocycliques, p. ex. indazole
• A61K 31/4162 - 1,2-Diazoles condensés avec des systèmes hétérocycliques
• A61P 27/02 - Agents ophtalmiques

Abrégé

The present disclosure refers to a method for a specific, versatile and sensitive detection of IFN-/virus-induced genes, a method for quantifying IFN potency and activity in a pharmaceutical preparation or biological sample, a method for distinguishing between IFN- and viral induction, and/ or for distinguishing between different viruses, and a method for the quantification of virus activity. Also, the invention provides the necessary molecular tools like expression active response constructs, suitable cell lines, an array to perform the method and a kit.

Classes IPC  ?

• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
• A61K 38/21 - Interférons
• C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus

Abrégé

The present invention relates to fluorescent proteins, in particular green fluorescent proteins (GFPs), with increased activity in cells, and thus increased signal strength. A further aspect of the present invention relates to the use of peptides for increasing the expression and/or stability of a protein in a cell.

Classes IPC  ?

• C12N 15/12 - Gènes codant pour des protéines animales
• A61K 49/00 - Préparations pour examen in vivo
• C07K 14/435 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains

Abrégé

The present invention relates to a method for increasing the expression of a protein in eukaryotic cells by reducing the number of RNase L cleavage sites in the coding and/or non-coding region of the nucleic acid sequence of said protein. Furthermore, it relates to nucleic acid sequences exhibiting a reduced number of RNase L cleavage sites as well as to the proteins translated from such sequences.

Classes IPC  ?

• C12N 15/68 - Stabilisation du vecteur
• C12N 9/22 - Ribonucléases

Abrégé

The present invention relates to a method for increasing the expression of a protein in cells, preferably in eukaryotic cells, by reducing the number of RNase L cleavage sites in the coding and/or non-coding region of the nucleic acid sequence of said protein. Furthermore, it relates to nucleic acid sequences exhibiting a reduced number of RNase L cleavage sites as well as to the proteins translated from such sequences.

Classes IPC  ?

• C12N 15/68 - Stabilisation du vecteur
• C12N 9/22 - Ribonucléases
• C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales

Abrégé

The present invention relates to regulated gene expression and reporter gene technology. It discloses a simple cloning-free method of generating a transcriptionally and/or post-transcriptionally controllable expression active linear reporter or gene construct with any desired introduced sequences. This method comprises a PCR amplification of a source DNA comprising a reporter or gene DNA sequence using (i) a forward primer comprising a first sequence part at the 3' end complementary to a region of the source DNA upstream of the reporter or gene DNA sequence, and a second sequence part at the 5' end comprising one or more introduced sequence(s), preferably transcriptional control sequence(s); and (ii) a reverse primer complementary to a region of the source DNA downstream of the reporter or gene DNA sequence.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

The present invention relates to expression vector comprising (a) a promoter region comprising a non-inducible constitutively active ribosomal protein gene promoter, (b) an operably linked reporter or gene sequence, and (c) a 3' untranslated region (3' UTR), which are suitable means for an selective assessment of post-transcriptional regulation, post-transcriptional control elements and factors as well as for identifying compounds that effect post-transcription. The present invention furthermore relates to arrays, expression vector libraries and cell lines containing the expression vector(s). The present invention furthermore relates to a method and kit for identifying compounds that affect post-transcriptional regulation of reporter(s) or gene(s), that utilize the expression vector(s).

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12N 15/79 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes

Abrégé

The present invention relates to expression vector comprising (a) a promoter
region comprising a non-inducible
constitutively active ribosomal protein gene promoter, (b) an operably linked
reporter or gene sequence, and (c) a 3' untranslated
region (3' UTR), which are suitable means for an selective assessment of post-
transcriptional regulation, post-transcriptional
con-trol elements and factors as well as for identifying compounds that effect
post-transcription. The present invention furthermore
re-lates to arrays, expression vector libraries and cell lines containing the
expression vector(s). The present invention furthermore
re-lates to a method and kit for identifying compounds that affect post-
transcriptional regulation of reporter(s) or gene(s), that utilize
the expression vector(s).

Classes IPC  ?

• C12N 15/79 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes

Abrégé

The present invention relates to a method for increasing the expression of a
protein in cells, preferably in
eukaryot-ic cells, by reducing the number of RNase L cleavage sites in the
coding and/or non-coding region of the nucleic acid sequence of
said protein. Furthermore, it relates to nucleic acid sequences exhibiting a
reduced number of RNase L cleavage sites as well as to
the proteins translated from such sequences.

Classes IPC  ?

• C12N 9/22 - Ribonucléases
• C12N 15/68 - Stabilisation du vecteur
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
• C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion

Abrégé

The present invention relates to fluorescent proteins, in particular green fluorescent proteins (GFPs), with increased activity in cells, and thus increased signal strength. A further aspect of the present invention relates to the use of peptides for increasing the expression and/or stability of a protein in a cell.

Classes IPC  ?

• A61K 49/00 - Préparations pour examen in vivo
• C07K 14/435 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains
• C12N 15/12 - Gènes codant pour des protéines animales