Abrégé

The present invention provides a hydrogel manufacturing apparatus capable of suppressing contamination of impurities from formation of a hydrogel until usage thereof. A hydrogel manufacturing apparatus (100) comprises: a first flow path (212) through which a hydrogel precursor flows; a second flow path (222) through which a gelling agent for gelling the hydrogel precursor flows; a first merging point (216) between the first flow path (212) and the second flow path (222); a housing container (400) for housing a hydrogel formed by contact between the hydrogel precursor and the gelling agent; and a hydrogel flow path (300) for connecting at least the housing container (400) and the first merging point (216) in a closed manner.

Classes IPC  ?

• B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• D01F 9/04 - Filaments, ou similaires, faits par l’homme, formés d’autres substancesLeur fabricationAppareils spécialement adaptés à la fabrication de filaments de carbone d'alginates

Abrégé

Provided is a method for producing a cell capsule capable of alleviating an adverse effect on a cell. This method for producing a cell capsule includes continuously or intermittently causing a hydrogel or a hydrogel precursor covering a cell to flow into a collection solution. The collection solution contains a pH buffer.

Classes IPC  ?

• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses
• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• C12N 11/10 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un hydrate de carbone

Abrégé

A cell population comprises a large number of cell aggregations and a hydrogel encapsulating the large number of cell aggregations. A coefficient of variation obtained by dividing a standard deviation of sizes of the large number of cell aggregations by a mean value of the sizes of the large number of cell masses is 0.50 or less. Alternatively and/or in addition there to, a coefficient of variation obtained by dividing a standard deviation of aspect ratios of shapes of the large number of cell aggregations by a mean value of the aspect ratios of the shapes of the large number of cell aggregations is 0.30 or less.

Classes IPC  ?

• C12N 5/073 - Cellules ou tissus embryonnairesCellules fœtales ou tissus fœtaux

Abrégé

The present invention provides a method for producing a cell population capable of providing an improved method for causing a cell to take up a substance to be introduced. This method for producing a cell population comprises covering a substance to be delivered and a cell by a semipermeable membrane (14) and forming a cell capsule (10), and culturing the cell in the cell capsule (10).

Classes IPC  ?

• C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
• C12N 15/88 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation utilisant la micro-encapsulation, p. ex. utilisant des vésicules liposomiques

Abrégé

Provided is a method for producing a novel cell population containing CD56-positive immune cells. The method for producing a cell population includes: forming a cell capsule (10) by covering a core (12), in which CD56-positive immune cells or precursor cells of CD56-positive immune cells are suspended, with a semipermeable membrane (14); and culturing the cell capsule (10) by immersing the cell capsule (10) in a medium.

Classes IPC  ?

• C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses

Abrégé

Provided is a structure with which a lung progenitor cells can proliferate while retaining an ability to differentiate into an alveolar epithelial cell and an airway epithelial cell. A structure of the present invention includes a core portion that includes a cell population, and an outer shell portion that covers at least a part of the core portion and contains a hydrogel. The structure has a storage elastic modulus of 150 Pa or more and/or the hydrogel contains alginic acid. The cell population contains an alveolar epithelial cell, an airway epithelial cell, and/or a progenitor cell thereof.

Classes IPC  ?

• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet

Abrégé

Provided is a method for producing cells or cell aggregates and/or cell products, the method facilitating removal of hydrogel-derived substances. The present invention provides a method for producing cells or cell aggregates and/or cell products, the method including: employing a capsule containing a core base material and a shell that covers the core base material to culture cells or cell aggregates in the core base material, thus obtaining cells or cell aggregates and/or cell products; and physically destroying or cutting at least the shell to obtain the cells or the cell aggregates and/or the cell products.

Classes IPC  ?

• C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
• C12N 5/0735 - Cellules souches embryonnairesCellules germinales embryonnaires
• C07K 14/745 - Facteurs de coagulation sanguine ou de fibrinolyse

Abrégé

The purpose of the present invention is to provide an exosome production method which makes it possible to produce an exosome with high efficiency. The exosome production method comprises: a step for culturing a cell (12) using a cell fiber (10) comprising a hydrogel (14) having a hollow fiber-like form and the cell (12) encapsulated in the inner cavity of the hydrogel; and a step for collecting an exosome released from the cell (12).

Classes IPC  ?

• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• C12N 15/88 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation utilisant la micro-encapsulation, p. ex. utilisant des vésicules liposomiques
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

The present invention provides a method for mass producing cell aggregates such as artificial organoids through an approach that differs entirely from previous methods. Specifically, provided is a method for producing cell aggregates that includes a step in which a mixture of mesenchymal stem cells, vascular endothelial cells, and organ cells are filled into microfibers, and the mixture is suspension cultured to form cell aggregates.

Classes IPC  ?

• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• A61K 35/28 - Moelle osseuseCellules souches hématopoïétiquesCellules souches mésenchymateuses de toutes origines, p. ex. cellules souches dérivées de tissu adipeux
• A61K 35/407 - FoieHépatocytes
• A61K 35/44 - VaisseauxCellules de muscles vasculaires lissesCellules endothélialesCellules progénitrices endothéliales
• A61K 35/545 - Cellules souches embryonnairesCellules souches pluripotentesCellules souches pluripotentes induitesCellules souches non caractérisées
• A61L 27/38 - Cellules animales
• A61P 1/16 - Médicaments pour le traitement des troubles du tractus alimentaire ou de l'appareil digestif des troubles de la vésicule biliaire ou du foie, p. ex. protecteurs hépatiques, cholagogues, cholélitholytiques
• A61P 43/00 - Médicaments pour des utilisations spécifiques, non prévus dans les groupes
• C12N 5/0775 - Cellules souches mésenchymateusesCellules souches dérivées du tissu adipeux

Abrégé

Provided is a novel structure containing mesenchymal stem cells that can be used in various applications. A hydrogel fiber (10) comprises a hydrogel (14) that contains mesenchymal stem cells.

Classes IPC  ?

• A61L 27/38 - Cellules animales
• A61L 27/20 - Polysaccharides
• A61L 27/24 - Collagène
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
• A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
• A61L 27/52 - Hydrogels ou hydrocolloïdes
• A61P 1/04 - Médicaments pour le traitement des troubles du tractus alimentaire ou de l'appareil digestif des ulcères, des gastrites ou des œsophagites par reflux, p. ex. antiacides, antisécrétoires, protecteurs de la muqueuse

Abrégé

A scaffold for culturing a cell comprises: a hydrogel; and a plasma-derived or platelet-derived component or a fibrin-containing material adhered to the hydrogel.

Classes IPC  ?

• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
• C12N 5/0775 - Cellules souches mésenchymateusesCellules souches dérivées du tissu adipeux

Abrégé

This cell population includes a large number of cell clusters and a hydrogel that covers the large number of cell clusters. A coefficient of variation obtained by dividing the standard deviation of the size of the cell clusters by the average value of the size of the cell clusters is not more than 0.50. Alternatively, or in addition thereto, a coefficient of variation obtained by dividing the standard deviation of the aspect ratios of the shapes of the cell clusters by the average value of the aspect ratios of the shapes of the cell clusters is not more than 0.30.

Classes IPC  ?

• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains

Abrégé

Provided is a structure for enabling proliferation of lung progenitor cells while maintaining the ability of the lung progenitor cells to differentiate into alveolar epithelial cells and airway epithelial cells.　This structure is provided with a core part that encapsulates a cell population and an outer shell part that at least partially covers the core part and that contains a hydrogel, the structure having a storage elastic modulus of 150 Pa or above and/or alginic acid being contained in the hydrogel, and the cell population including alveolar epithelial cells, airway epithelial cells, and/or progenitor cells thereof.

Classes IPC  ?

• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
• C07K 14/78 - Peptides du tissu connectif, p. ex. collagène, élastine, laminine, fibronectine, vitronectine ou globuline insoluble à froid [CIG]

Abrégé

A cell fiber production system comprises: a first flow path through which a first fluid containing a cell flows; a second flow path for allowing a second fluid for preparing a hydrogel to converge around the first fluid in the first flow path; and an ejection port through which at least the first fluid and the second fluid are discharged together. The cell fiber production system is constructed that, at a start stage of production of a cell fiber, the first fluid is delivered after the second fluid reaches at least a convergence point between the first flow path and the second flow path.

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses
• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains

Abrégé

Provided is a novel structure containing mesenchymal stem cells that can be used in various applications. A hydrogel fiber (10) comprises a hydrogel (14) that contains mesenchymal stem cells.

Classes IPC  ?

• A61K 35/28 - Moelle osseuseCellules souches hématopoïétiquesCellules souches mésenchymateuses de toutes origines, p. ex. cellules souches dérivées de tissu adipeux
• A61K 9/70 - Bases pour bande, feuille ou filament
• A61K 47/36 - PolysaccharidesLeurs dérivés, p. ex. gommes, amidon, alginate, dextrine, acide hyaluronique, chitosane, inuline, agar-agar ou pectine
• A61K 47/42 - ProtéinesPolypeptidesLeurs produits de dégradationLeurs dérivés p. ex. albumine, gélatine ou zéine
• A61P 1/04 - Médicaments pour le traitement des troubles du tractus alimentaire ou de l'appareil digestif des ulcères, des gastrites ou des œsophagites par reflux, p. ex. antiacides, antisécrétoires, protecteurs de la muqueuse

Abrégé

A scaffold (10) for culturing cells has a hydrogel (12) and a plasma-derived or platelet-derived component or a fibrin-containing substance (14) attached to the hydrogel (12).

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• C12N 5/0775 - Cellules souches mésenchymateusesCellules souches dérivées du tissu adipeux

Abrégé

A storage body (10) has a storage modulus (G') of 100 Pa or more at a frequency of 1 Hz, and includes a hydrogel (14) having cells or cell aggregates (12) enclosed therein. The hydrogel (14) has a cylindrical shape extending continuously.

Classes IPC  ?

• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
• C12N 5/077 - Cellules mésenchymateuses, p. ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires

Produits et services

Chemicals used in industry; Protein in raw material form for scientific and medical research; Enzymes for scientific and research purposes; Bacteriological preparations, other than for medical and veterinary use; Bacteriological preparations for acetification other than for medical or veterinary use; Biological preparations, other than for medical or veterinary purposes; Biological tissue cultures, other than for medical or veterinary purposes; Chemical preparations for scientific purposes, other than for medical or veterinary use; Cultures of microorganisms, other than for medical and veterinary use; Milk ferments for chemical purposes for industrial purposes; Preparations of microorganisms, other than for medical and veterinary use; Stem cells, other than for medical or veterinary purposes; none of the foregoing for use in agriculture, horticulture or forestry Dietary supplements; Dietetic beverages adapted for medical purposes; Dietetic food adapted for medical purposes; Food for babies; Dietary supplements for animals, other than drugs; Pharmaceutical preparations for the treatment of diseases and disorders of the metabolic system, cardiovascular system, respiratory system, nervous system, skin system, digestive system, endocrine system, urinary system, blood system, reproductive system, namely, pharmaceutical preparations for the treatment of cancers, autoimmune diseases, and neurological disorders; Medicines for veterinary purposes for the treatment of diseases and disorders of the metabolic system, cardiovascular system, respiratory system, nervous system, skin system, digestive system, endocrine system, urinary system, blood system, reproductive system, namely, pharmaceutical preparations for the treatment of cancers, autoimmune diseases, and neurological disorders; Biological preparations for the treatment of diseases and disorders of the metabolic system, cardiovascular system, respiratory system, nervous system, skin system, digestive system, endocrine system, urinary system, blood system, reproductive system, namely, pharmaceutical preparations for the treatment of cancers, autoimmune diseases, and neurological disorders; Biological preparations for the treatment of cancers, autoimmune diseases, and neurological disorders for veterinary purposes; Reagent paper for medical purposes Providing computer programs on data networks, namely, providing temporary use of online non-downloadable software for use in operation control of laboratory apparatus and instruments; Rental of laboratory apparatus and instruments; Bacteriological research; Biological research; Chemical analysis; Chemical research; Chemistry services, namely, chemistry consultation, research in the field of chemistry, laboratory research and analysis in the field of chemistry; Research and development of new products for others; Scientific laboratory services; Scientific research; Designing of machines, apparatus, instruments, including their parts, or systems composed of such machines, apparatus and instruments; Computer programming; Technological advice relating to the operation of computers; Testing, inspection or research of pharmaceuticals, cosmetics or foodstuffs; Testing, inspection or research on cell systems in the fields of agriculture, livestock breeding or fisheries; Testing the functionality of machines, apparatus and instruments; research on machines, apparatus and instruments; Rental of measuring apparatus; Rental of computers

Abrégé

An additional method for producing an antibody was needed. Provided are: a chemically crosslinked alginate gel fiber which is for producing an antibody and in which an antibody-producing cell (for example, an antibody-producing CHO cell) is contained in a core layer; and a method for producing an antibody using said alginate gel fiber.

Classes IPC  ?

• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses
• C07K 14/78 - Peptides du tissu connectif, p. ex. collagène, élastine, laminine, fibronectine, vitronectine ou globuline insoluble à froid [CIG]
• C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
• C12N 15/13 - Immunoglobulines
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
• C12P 21/08 - Anticorps monoclonaux

Abrégé

This cellular fiber production system has a first flow path through which a first fluid containing cells flows, a second flow path which causes a second fluid for preparation of a hydrogel to merge around the first fluid in the first flow path, and an outlet opening through which at least the first and second fluids are discharged together. This cellular fiber production system is configured so that during the start of production of cellular fibers, the first fluid is fed at least after the second fluid reaches a point where the first and second flow paths merge together.

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/26 - Inoculateur ou échantillonneur
• C12N 5/07 - Cellules animales ou tissus animaux
• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses