The disclosure provides polypeptides and encoding nucleic acids of engineered acyl coenzyme-A ligases, engineered In acetylating aldehyde dehydrogenases or both. The disclosure also provides cells expressing an engineered form of the acyl coenzyme-A ligases, acetylating aldehyde dehydrogenases or both. The disclosure further provides methods for producing a bioderived compound, such as 6-hydroxyhexanoyl coenzyme-A, 6-hydroxy hexanal, 1,6 hexanediol, 6-aminocaproyl-CoA, 6-aminocaproate semialdehyde, hexamethylenediamine, and products derived therefrom comprising culturing cells expressing an engineered acyl coenzyme-A ligases, engineered acetylating aldehyde dehydrogenases or both.
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine
2.
MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS
The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms of the invention having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.
C12P 13/00 - Préparation de composés organiques contenant de l'azote
C12P 17/10 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'azote comme unique hétéro-atome du cycle
The disclosure relates to methods for the production of natural lactones by bacteria under physiological conditions. The methods employ ybgC proteins having lactonizing activity.
C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
17 - Produits en caoutchouc ou en matières plastiques; matières à calfeutrer et à isoler
18 - Cuir et imitations du cuir
22 - Cordes; filets; tentes, auvents, voiles et sacs; matières de rembourrage
23 - Fils à usage textile
24 - Tissus et produits textiles
25 - Vêtements; chaussures; chapellerie
Produits et services
Polymer compositions used in the manufacture of commercial, industrial and domestic goods; polymer precursors used in the manufacture of commercial, industrial and domestic goods; polymers, biopolymers, polymeric additives and biopolymeric additives for use in the manufacture of industrial products and consumer and household products; chemicals for use in industry; chemicals used in the manufacture of fabric or textiles; and polyamide. Resins in bars, blocks, pellets, rods, sheets and tubes for general industrial use; resins in extruded form for general industrial use; and semi-processed synthetic resins. Tote bags, all-purpose sports bags, all-purpose carrying bags, carry-on bags, weekend bags, sack packs, namely, drawstring bags used as backpacks, beach bags, toiletry bags sold empty, cosmetic bags sold empty, shopping bags, wheeled shopping bags, reusable shopping bags, sling bags for carrying infants, knitted bags not of precious metals, travel bags, school book bags, duffle bags, wheeled duffle bags, messenger bags, diaper bags, gym bags, purses, handbags, cross-body bags, drawstring bags, shoulder bags, waist bags, wristlet bags, backpacks, rucksacks, wallets, not of precious metal, briefcases, attaché cases, clutch bags, hip bags, umbrellas, credit card cases, business card cases, key cases, pocket wallets, haversacks, suitcases, daypacks, suit bags, garment bags for travel, and luggage, all made of bio-nylon as a component. Textile fibers; yarn fibers; synthetic fibers for textile use; raw textile fibers; woven fibers of bio-nylon for textile use; non-woven polymeric fibers for conversion into a variety of industrial and consumer goods, namely, non-woven polymeric fibers for textile use and industrial use; woven polymeric fibers for conversion into a variety of industrial and consumer goods, namely, woven polymeric fibers for textile use and industrial use; and synthetic fibers and filaments for use in the manufacture of fabrics, textiles, yarns, and carpets. Thread; yarn; synthetic thread; synthetic yarn; thread for textile use; yarn for textile use; synthetic fiber thread and yarn; and yarn and thread for textile purposes. Bio-nylon fabric for use as a textile in the manufacture of clothing; textile fabrics for use in the manufacture of garments, bags, jackets, gloves, and apparel; fabrics made of bio-nylon for textile use; fabrics for textile use. Clothing, headwear, footwear and outerwear for men, women and children, namely, shirts, t-shirts, sweat shirts, sweat pants, dresses, eveningwear, blouses, neckties, sweaters, cardigans, jerseys, overalls, suits, skirts, shorts, trousers, pants, yoga pants, jeans, bodysuits, leggings, leotards, tights, hosiery, swimwear, bathing trunks, bathing suits, pajamas, nightwear, robes, bras, underwear, panties, girdles, thermal underwear, socks, base layer tops and bottoms, coats, raincoats, jackets, vests, shawls, gloves, slippers, shoes, sneakers, athletic footwear, overshoes, sandals, boots, hats and caps being headwear, headbands being clothing, suspenders, scarves, knee warmers, neck tubes, hand warmers, gaiters, and clothing belts; and finished textile linings for garments, all made of bio-nylon as a component.
5.
METHODS AND ORGANISMS WITH INCREASED CARBON FLUX EFFICIENCIES
The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides novel multifunctional fatty acid derivative molecules such as e.g., fatty triols, fatty tetrols, dihydroxy fatty acids, etc. The disclosure further provides derivatives of the disclosed multifunctional molecules which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc. The disclosure further provides biochemical pathways, recombinant microorganisms and methods for the biological production of various multifunctional fatty acid derivatives.
C07C 68/00 - Préparation des esters de l'acide carbonique ou de l'acide formique halogéné
C07C 69/22 - Esters d'acides acycliques monocarboxyliques saturés dont le groupe carboxyle est lié à un atome de carbone acyclique ou à l'hydrogène avec au moins trois atomes de carbone dans la partie acide
C07C 69/30 - Esters d'acides acycliques monocarboxyliques saturés dont le groupe carboxyle est lié à un atome de carbone acyclique ou à l'hydrogène avec au moins trois atomes de carbone dans la partie acide estérifiés par des composés trihydroxylés
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
C07C 209/62 - Préparation de composés contenant des groupes amino liés à un squelette carboné par clivage de liaisons carbone-azote, soufre-azote ou phosphore-azote, p. ex. hydrolyse d'amides, N-déalkylation d'amines ou de composés d'ammonium quaternaire
C08G 69/26 - Polyamides dérivés, soit des acides amino-carboxyliques, soit de polyamines et d'acides polycarboxyliques dérivés de polyamines et d'acides polycarboxyliques
C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 13/00 - Préparation de composés organiques contenant de l'azote
8.
ENGINEERED MICROORGANISMS WITH G3P ->3PG ENZYME AND/OR FRUCTOSE-1,6-BISPHOSPHATASE INCLUDING THOSE HAVING SYNTHETIC OR ENHANCED METHYLOTROPHY
Described herein are engineered cells including ones having synthetic methylotrophy which include an NADH-dependent enzyme capable of converting G3P to 3PG (e.g., B. methanolicus gapN) and/or fructose-1,6-bisphosphatase, along with hexulose-6-phosphate synthase, 6-phospho-3-hexuloisomerase, a phosphoketolase, or a combination thereof. Engineered cells of the disclosure beneficially maintain adequate pool sizes of phosphorylated C3 and/or C4 compounds, and/or provide increased levels of NADPH. As such, the modifications allow for the generation of C6 compounds from C1 (e.g., a methanol feedstod) and C5 compounds, the regeneration of C5 compounds from C6 compounds by carbon rearrangement, and an improved balance between regeneration of C5 compounds and lower glycolysis. In turn, this allows the engineered microorganism to generate sufficient quantities of metabolic precursors (e.g., acetyl-CoA) which can be used in a bioproduct pathway, and the engineered cells can include further modifications to those pathway enzymes allowing for production of a desired bioproduct.
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C07K 14/195 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries
The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.
The disclosure provides polypeptides and encoding nucleic acids of engineered aldehyde dehydrogenases. The disclosure also provides cells expressing an engineered form of the aldehyde dehydrogenase. The disclosure further provides methods for producing a bioderived compound, such as 3-hydroxybutyraldehyde, 1,3-butanediol, 4-hydroxybutyraldehyde, 1,4-butanediol, comprising culturing cells expressing an engineered aldehyde dehydrogenase.
The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12P 13/00 - Préparation de composés organiques contenant de l'azote
C12P 17/04 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à cinq chaînons, p. ex. griséofulvine
C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine
16.
1,3-BUTYLENE GLYCOL COMPOSITIONS AND METHODS OF USE
Provided herein are methods, compositions, and uses for a bioderived 1,3 butylene glycol (1,3-BG) characterized by one or more odor-active regions on a chromatograph. Various methods of producing and characterizing an odorless and a fragrant bioderived 1,3-BG for cosmetic uses are also provided.
The disclosure provides polypeptides and encoding nucleic acids of engineered aldehyde dehydrogenases. The disclosure also provides cells expressing an engineered form of the aldehyde dehydrogenase. The disclosure further provides methods for producing a bioderived compound, such as 3-hydroxybutyraldehyde, 1,3-butanediol, 4-hydroxybutyraldehyde, 1,4-butanediol, comprising culturing cells expressing an engineered aldehyde dehydrogenase.
MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING 1,4-BUTANEDIOL RELATED THERETO
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
C08G 63/06 - Polyesters dérivés soit d'acides hydroxycarboxyliques, soit d'acides polycarboxyliques et de composés polyhydroxylés dérivés des acides hydroxycarboxyliques
C08G 63/12 - Polyesters dérivés soit d'acides hydroxycarboxyliques, soit d'acides polycarboxyliques et de composés polyhydroxylés dérivés d'acides polycarboxyliques et de composés polyhydroxylés
C08G 63/66 - Polyesters contenant de l'oxygène sous forme de groupes éther
C08G 69/26 - Polyamides dérivés, soit des acides amino-carboxyliques, soit de polyamines et d'acides polycarboxyliques dérivés de polyamines et d'acides polycarboxyliques
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
Disclosed are biosynthetic methods and engineered microorganisms that enhance or improve the biosynthesis of 6-aminocaproate, hexamethylenediamine, caproic acid, caprolactone, or caprolactam. The engineered microorganisms are modified to include, for example, upredulated and/or exogenous transporters for 6-aminocaproate, deletions and/or downregulated importers for 6-aminocaproate, upregulated and/or exogenous glutamate dehydrogenase, and/or deletions and/or downregulation of rcsA and/or cpsBG. Other engineered microorganisms may have disruptions of endogenous transporters for 6-aminocaproate.
The disclosure provides embodiments directed to a 1,3-butylene glycol (BG) product that has a neutral taste, a composition comprising the BG product described here, and methods of using the BG product and compositions comprising the BG products. BG products described here demonstrate exemplary qualities and characteristics of being natural, sustainable, and having a neutral taste for use in food products (including beverages). Such BG compositions of the description provide a BG product that does not mask or alter the taste of the food products. The BG products and compositions comprising such BG products as disclosed here provide advantageous properties while maintaining a neutral taste.
The present invention provides a method for separating hexamethylene diamine (HMDA) from an aqueous solution, comprising the steps: a) providing an HMDA- containing aqueous solution, wherein said aqueous solution is a fermentation broth, b) extracting the HMDA-containing aqueous solution with a monovalent alcohol having 4 to 8 carbon atoms, c) obtaining an HMDA-containing alcoholic phase, and d) distilling the HMDA-containing alcoholic phase at a pressure of 1-1000 mbar and separating the HMDA from the monovalent alcohol.
Method for separating 1,6-hexamethylenediamine (HMDA) from an aqueous solution, comprising the steps: a) providing HMDA-containing aqueous solution, wherein said aqueous solution is a fermentation broth, b) extracting the HMDA-containing aqueous solution with a solvent mixture (i) at least one monovalent alcohol having 4 to 7 carbon atoms and (ii) at least one alkane having 5 to 7 carbon atoms, wherein the alkane content of the solvent mixture is in the range from 2 wt% to 50 wt% based on the total weight of all alcohols and alkanes present in the solvent mixture; c) obtaining an HMDA- containing solvent mixture as separatephase, and d) distilling the HMDA-containing solvent mixture and separating the HMDA from the solvent mixture.
The disclosure relates to the field of specialty chemicals. In particular, the disclosure provides novel 1,3-fatty-diol compounds and derivatives thereof which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc.
C07H 15/10 - Radicaux acycliques non substitués par des structures cycliques liés à un atome d'oxygène d'un radical saccharide contenant des liaisons non saturées carbone-carbone
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
17 - Produits en caoutchouc ou en matières plastiques; matières à calfeutrer et à isoler
22 - Cordes; filets; tentes, auvents, voiles et sacs; matières de rembourrage
23 - Fils à usage textile
24 - Tissus et produits textiles
25 - Vêtements; chaussures; chapellerie
Produits et services
Polymer base compositions used in the manufacture of commercial, industrial and domestic goods; Polymer compositions used in the manufacture of commercial and industrial goods; Polymer base compositions used in the manufacture of goods, namely, clothing, exercise apparel, compression garments, home furnishing; Polymers, biopolymers, polymeric additives and biopolymeric additives for use in the manufacture of industrial products, consumer and household products, sealants or packaging; Chemicals for use in industry; Chemicals used in the manufacture of fabric or textiles; Polyamide Resins in bars, blocks, pellets, rods, sheets and tubes for general industrial use; Resins in extruded form for general industrial use; Semi-processed synthetic resins Textile fibers; Yarn fibers; Synthetic fibers for textile use; Raw textile fibers; Woven fibers of nylon and bio-nylon for textile use; Non-woven polymeric fibers for conversion into a variety of industrial and consumer goods; Woven polymeric fibers for conversion into a variety of industrial and consumer goods; Synthetic fibers and filaments for use in the manufacture of fabrics, textiles, yarns, and carpets Thread; Yarn; Synthetic thread; Synthetic yarn; Thread for textile use; Yarn for textile use; Synthetic fiber thread and yarn; Yarn and thread for textile purposes; Yarn made of nylon, bio-nylon, and biofabricated materials for textile use Nylon fabric; Nylon and Bio-nylon fabric for use as a textile in the manufacture of clothing; Textile fabrics for use in the manufacture of garments, bags, jackets, gloves, and apparel; Fabrics made of nylon and bio-nylon for textile use; Fabrics for textile use; Nylon and Bio-nylon fabric for use as a textile in the manufacture of tote bags, all-purpose sports bags, all-purpose carrying bags, carry-on bags, weekend bags, sack packs, namely, drawstring bags used as backpacks, beach bags, toiletry bags sold empty, cosmetic bags sold empty, shopping bags, wheeled shopping bags, reusable shopping bags, sling bags for carrying infants, knitted bags not of precious metals, travel bags, school book bags, duffle bags, wheeled duffle bags, messenger bags, diaper bags, gym bags, purses, handbags, cross-body bags, drawstring bags; Nylon and Bio-nylon fabric for use as a textile in the manufacture of shoulder bags, waist bags, wristlet bags, backpacks, rucksacks, wallets, not of precious metal, briefcases, attaché cases, clutch bags, hip bags, umbrellas, credit card cases, business card cases, key cases, pocket wallets, haversacks, suitcases, daypacks, suit bags, garment bags for travel, and luggage Fabric sold as an integral component of finished clothing items, namely, headwear, footwear and outerwear for men, women and children, namely, shirts, t-shirts, sweat shirts, sweat pants, dresses, eveningwear being gowns, blouses, neckties, sweaters, cardigans, jerseys, overalls, suits, skirts, shorts, trousers, pants, yoga pants, jeans, bodysuits, leggings, leotards, tights, hosiery, swimwear, bathing trunks, bathing suits, pajamas, nightwear, robes, bras, underwear, panties, shapewear, namely, girdles, thermal underwear, socks, base layer tops and bottoms, coats, raincoats, jackets, vests; Fabric sold as an integral component of finished clothing items, namely, shawls, gloves, slippers, shoes, sneakers, athletic footwear, overshoes, sandals, boots, hats and caps being headwear, headbands being clothing, suspenders, scarves, knee warmers, neck tubes, hand warmers, gaiters, and clothing belts; Finished textile linings for garments
25.
NEUTRAL ODOR 1,3-BUTYLENE GLYCOL COMPOSITIONS AND METHODS OF USE THEREOF
The disclosure provides embodiments directed to a 1,3-butylene glycol (BG) product that has a neutral odor, a composition comprising the BG product described here, and methods of using the BG product and compositions comprising the BG products. BG products described here demonstrate exemplary qualities and characteristics of being natural, sustainable, and having a neutral odor for use in cosmetics, cosmeceuticals, and personal care products. Such BG compositions of the description provide a BG product that does not mask or alter the fragrance notes of the cosmetics, cosmeceuticals, and personal care products. The BG products and compositions comprising such BG products as disclosed here are cost-effective while maintaining a neutral odor.
Recombinant microbes comprising a novel pathway for producing fatty acid derivatives, such as fatty acid methyl esters, fatty acid ethyl esters, fatty alcohols, and fatty alcohol acetates, while generating low levels of 3-hydroxy fatty acid byproducts are provided herein. The addition of a heterologous R-3-hydroxy-acyl-CoA dehydratase or a heterologous R-specific enoyl-CoA hydratase and optionally a heterologous trans-2-enoyl-CoA reductase to a recombinant microbe reduces the amount of 3-hydroxy fatty acid byproducts produced by the acyl-ACP dependent fatty acid biosynthesis pathway of the recombinant microbe. Methods of producing the fatty acid derivatives are also provided, in addition to cell cultures and fatty acid derivative compositions produced by the recombinant microbes. The fatty acid derivatives produced by the recombinant microbes may be used to produce fragrances, pheromones, pharmaceutical agents, nutraceuticals or precursors thereof.
The present disclosure relates to processes that combine microbial production of organic intermediates and subsequent synthetic transformation to provide compounds of industrial value, including compounds used in fragrances.
C12P 17/08 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle d'au moins sept chaînons, p. ex. zéaraléone, aglycones de macrolides
C12P 17/04 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à cinq chaînons, p. ex. griséofulvine
28.
3-HYDROXYBUTYRYL-COA DEHYDROGENASE VARIANTS AND METHODS OF USE
The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine
The disclosure provides polypeptides and encoding nucleic acids of engineered desaturases. The disclosure also provides cells expressing an engineered form of the desaturase or a desaturase as described herein. The disclosure further provides methods for producing a bioderived compound, such as fatty acid precursors, fatty acids, and/or derivatives thereof, comprising culturing cells expressing an engineered desaturase or a desaturase provided herein. The disclosure further provides methods for producing pheromones or pheromone precursors, comprising culturing cells expressing an engineered desaturase or a desaturase provided herein and obtaining the fatty acid precursors, fatty acids, and/or derivatives thereof bioderived therefrom. The disclosure further provides methods for producing fragrances or fragrance precursors, comprising culturing cells expressing an engineered desaturase or a desaturase provided herein and obtaining the fatty acid precursors, fatty acids, and/or derivatives thereof bioderived therefrom.
The disclosure provides polypeptides and encoding nucleic acids of engineered carboxylic acid reductases. The disclosure also provides cells expressing an engineered form of the carboxylic acid reductase or a carboxylic acid reductase as described herein. The disclosure further provides methods for producing a bioderived compound, such as fatty acid derivatives and/or products thereof, comprising culturing cells expressing an engineered carboxylic acid reductase or a carboxylic acid reductase provided herein. The disclosure further provides methods for producing pheromones or pheromone precursors, comprising culturing cells expressing an engineered carboxylic acid reductase or a carboxylic acid reductase provided herein and obtaining the fatty acid derivatives and/or products thereof bioderived therefrom.
The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.
The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.
C07C 31/125 - Alcools monohydroxyliques acycliques contenant de cinq à vingt-deux atomes de carbone
C07C 33/02 - Alcools acycliques à liaisons doubles carbone-carbone
C07C 53/126 - Acides contenant au moins cinq atomes de carbone
C07K 14/195 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries
C07K 14/24 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant d'Entérobacteriaceae (F), p. ex. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
The disclosure provides polypeptides and encoding nucleic acids of engineered formate dehydrogenases. The disclosure also provides cells expressing an engineered formate dehydrogenase. The disclosure further provides methods for producing a bioderived compound comprising culturing cells expressing an engineered formate dehydrogenase. Where the engineered formate dehydrogenase is capable of catalyzing a conversion.
The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.
The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
The disclosure provides polypeptides and encoding nucleic acids of engineered diamine exporters. The disclosure also provides cells expressing engineered diamine exporter or exogenous diamine exporters. The disclosure further provides methods for diamine bioproduction.
The invention relates to a method for obtaining an amine from a composition by means of distillation and a composition obtainable by that method. The invention also relates to a method for the production of an isocyanate from the composition obtainable.
The invention relates to a method for the removal of water from and transport of at least one aliphatic diamine comprising the following steps: a) providing a first composition including 60 to 98 wt.-% water and 2 to 40 wt.-% of the at least one aliphatic diamine with two —NH2 groups per molecule, each of them bound to a primary or secondary carbon atom, b) removing water at least partially from said first composition by distillation at a first production location in a distillation apparatus comprising at least two distillation columns operating at different head pressures, thus generating a second composition comprising the diamine and ≤55 wt.-% water, wherein the vapours emerging from the distillation column operating at the higher head pressure are used to evaporate the liquid in the bottoms and/or the feed of a distillation column operating at the lower head pressure at least partially, c) transporting said second composition during a span of time t from the first production location to a second production location, wherein the span of time t including times for optional temporary storage is at least 6 h.
The invention relates to a method for the processing and transport of hexane-1,6-diamine or pentane-1,5-diamine comprising the steps of: a) Providing a first composition including 60 to 98 wt.-% water and 2 to 40 wt.-% hexane-1,6-diamine or pentane-1,5-diamine, b) partially removing water from the first composition by distillation at a first production location, whereby a second composition is generated, said second composition including the hexane-1,6-diamine or pentane-1,5-diamine and 1 wt.-% to 35 wt.-% water, and c) transporting said second composition during a span of time t from the first production location to a second production location, wherein the span of time t including times for optional temporary storage is at least 6 h.
The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides novel multifunctional fatty acid derivative molecules such as e.g., fatty triols, fatty tetrols, dihydroxy fatty acids, etc. The disclosure further provides derivatives of the disclosed multifunctional molecules which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc. The disclosure further provides biochemical pathways, recombinant microorganisms and methods for the biological production of various multifuctional fatty acid derivatives.
C07C 68/00 - Préparation des esters de l'acide carbonique ou de l'acide formique halogéné
C07C 69/22 - Esters d'acides acycliques monocarboxyliques saturés dont le groupe carboxyle est lié à un atome de carbone acyclique ou à l'hydrogène avec au moins trois atomes de carbone dans la partie acide
C07C 69/30 - Esters d'acides acycliques monocarboxyliques saturés dont le groupe carboxyle est lié à un atome de carbone acyclique ou à l'hydrogène avec au moins trois atomes de carbone dans la partie acide estérifiés par des composés trihydroxylés
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
42.
ENGINEERED ENZYMES AND METHODS OF MAKING AND USING
The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semialdehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.
The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms of the invention having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.
C12P 13/00 - Préparation de composés organiques contenant de l'azote
C12P 17/10 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'azote comme unique hétéro-atome du cycle
44.
MICROORGANISMS AND METHODS FOR REDUCING BY-PRODUCTS
The present disclosure provides nonrnaturally occurring microbial organisms having eliminate, substantially eliminate, or reduced production of unwanted by-products (e.g, 3HB, acetate, and others) and/or to enhance or increase carbon flux through 3 HB-CoA, and/or 3HBAId, and/or the CoA thioesters or acyl-CoAs of unwanted by-products, which can increase production of acetyl-CoA derived compounds (e.g, 1,3-BDO, MMA, and (3R)-hydroxybutyl (3R)-hydroxybutyrate, or any other acetyl-CoA derived compounds), and products made from any of these compounds. Also provided are one or more exogenous nucleic acids encoding enzymes that can eliminate, substantially eliminate, or reduce production of unwanted by-products (e.g, 3HB, acetate, and others) and/or that can enhance or increase carbon flux through 3HB-CoA, 3HBAId, and/or the CoA thioesters/acyl-CoAs of unwanted by-products.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 1/13 - Algues unicellulairesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase/isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The disclosure provides embodiments directed to a composition comprising a 1,3-butylene glycol (1,3-BG; such as but not limited to, bioBG) and one or more fragrance ingredients, which form a fragrance component of the composition. 1,3-BG demonstrates exemplary qualities and characteristics of being natural, sustainable, and substantially non-odorous, for use in fragrance compositions. Methods of using or applying such 1,3-BG fragrance compositions provide a cost-efficient, sustainable composition, since 1,3-BG does not mask or alter the fragrance notes of the fragrance ingredients allowing for the use of smaller quantities of fragrance ingredients. The 1,3-BG fragrance compositions of the disclosure also improve fragrance performance and reduces greenhouse gases, while avoiding any irritation, sensitization, or allergic reaction in humans.
The disclosure provides polypeptides and encoding nucleic acids of engineered acyl coenzyme-A ligases, engineered acetylating aldehyde dehydrogenases or both. The disclosure also provides cells expressing an engineered form of the acyl coenzyme-A ligases, acetylating aldehyde dehydrogenases or both. The disclosure further provides methods for producing a bioderived compound, such as 6-hydroxyhexanoyl coenzyme-A, 6 -hydroxy hexanal, 1,6 hexanediol, 6-aminocaproyl-CoA, 6-aminocaproate semialdehyde, hexamethylenediamine, and products derived therefrom comprising culturing cells expressing an engineered acyl coenzyme-A ligases, engineered acetylating aldehyde dehydrogenases or both.
The disclosure provides polypeptides and encoding nucleic acids of engineered phosphoketolases. The disclosure also provides cells expressing an engineered phosphoketolase. The disclosure further provides methods for producing a bioderived compound comprising culturing cells expressing an engineered phosphoketolase.
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes
49.
PROCESS AND SYSTEMS FOR OBTAINING 1,3-BUTANEDIOL FROM FERMENTATION BROTHS
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
C07C 29/94 - Emploi d'additifs, p. ex. pour la stabilisation
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
The disclosure relates to downstream processing of fatty alcohol (FALC) and provides a novel purification method that provides FALC at high purity and yield.
C07C 29/88 - SéparationPurificationStabilisationEmploi d'additifs par traitement donnant lieu à une modification chimique d'au moins un composé
C07C 29/17 - Préparation de composés comportant des groupes hydroxyle ou O-métal liés à un atome de carbone ne faisant pas partie d'un cycle aromatique à six chaînons par hydrogénation de liaisons doubles ou triples carbone-carbone
52.
MICROORGANISMS AND METHODS FOR PRODUCING (3R)-HYDROXYBUTYL (3R)-HYDROXYBUTYRATE
Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.
A23L 33/135 - Bactéries ou leurs dérivés, p. ex. probiotiques
A61K 31/22 - Esters, p. ex. nitroglycérine, sélénocyanates d'acides carboxyliques d'acides acycliques, p. ex. pravastatine
A61K 35/66 - Micro-organismes ou substances provenant de micro-organismes
A61K 36/064 - Saccharomycetales, p. ex. levure de boulanger
C07C 69/675 - Esters d'acides carboxyliques dont le groupe carboxyle estérifié est lié à un atome de carbone acyclique et dont l'un des groupes OH, O-métal, —CHO, céto, éther, acyloxy, des groupes , des groupes ou des groupes se trouve dans la partie acide d'acides saturés d'acides hydroxycarboxyliques saturés
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.
C12P 17/10 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'azote comme unique hétéro-atome du cycle
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
C12P 13/00 - Préparation de composés organiques contenant de l'azote
C12P 17/08 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle d'au moins sept chaînons, p. ex. zéaraléone, aglycones de macrolides
55.
Methods and organisms with increased carbon flux efficiencies
The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
C08G 63/06 - Polyesters dérivés soit d'acides hydroxycarboxyliques, soit d'acides polycarboxyliques et de composés polyhydroxylés dérivés des acides hydroxycarboxyliques
C08G 63/12 - Polyesters dérivés soit d'acides hydroxycarboxyliques, soit d'acides polycarboxyliques et de composés polyhydroxylés dérivés d'acides polycarboxyliques et de composés polyhydroxylés
C08G 63/66 - Polyesters contenant de l'oxygène sous forme de groupes éther
C08G 69/26 - Polyamides dérivés, soit des acides amino-carboxyliques, soit de polyamines et d'acides polycarboxyliques dérivés de polyamines et d'acides polycarboxyliques
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.
In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.
The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semi aldehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.
Provided herein are methods, compositions, and uses for a bioderived 1,3 butylene glycol (1,3-BG) characterized by one or more odor-active regions on a chromatograph. Various methods of producing and characterizing an odorless and a fragrant bioderived 1,3-BG for cosmetic uses are also provided.
MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING ADIPATE, 6-AMINOCAPROATE, HEXAMETHYLENEDIAMINE OR CAPROLACTAM RELATED THERETO
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
C12P 17/10 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'azote comme unique hétéro-atome du cycle
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
C12P 13/00 - Préparation de composés organiques contenant de l'azote
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C08G 69/08 - Polyamides dérivés, soit des acides amino-carboxyliques, soit de polyamines et d'acides polycarboxyliques dérivés d'acides aminocarboxyliques
A23L 29/00 - Aliments ou produits alimentaires contenant des additifsLeur préparation ou leur traitement
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
63.
NEUTRAL TASTE 1,3-BUTYLENE GLYCOL PRODUCTS AND COMPOSITIONS THEREOF
The disclosure provides embodiments directed to a 1,3-butylene glycol (BG) product that has a neutral taste, a composition comprising the BG product described here, and methods of using the BG product and compositions comprising the BG products. BG products described here demonstrate exemplary qualities and characteristics of being natural, sustainable, and having a neutral taste for use in food products (including beverages). Such BG compositions of the description provide a BG product that does not mask or alter the taste of the food products. The BG products and compositions comprising such BG products as disclosed here provide advantageous properties while maintaining a neutral taste.
The disclosure provides embodiments directed to a 1,3-butylene glycol (BG) product that has a neutral odor, a composition comprising the BG product described here, and methods of using the BG product and compositions comprising the BG products. BG products described here demonstrate exemplary qualities and characteristics of being natural, sustainable, and having a neutral odor for use in cosmetics, cosmeceuticals, and personal care products. Such BG compositions of the description provide a BG product that does not mask or alter the fragrance notes of the cosmetics, cosmeceuticals, and personal care products. The BG products and compositions comprising such BG products as disclosed here are cost-effective while maintaining a neutral odor.
A61K 8/02 - Cosmétiques ou préparations similaires pour la toilette caractérisés par une forme physique particulière
A61K 8/81 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des composés organiques macromoléculaires obtenus par des réactions faisant intervenir uniquement des liaisons insaturées carbone-carbone
Variant acyl-ACP thioesterases and recombinant cells, microorganisms, or microbes, including proteobacteria and cyanobacteria, comprising the variant acyl-ACP thioesterases, are provided herein. The recombinant microbes produce monounsaturated free fatty acids and/or derivatives thereof, including palmitoleic acid and palmitoleic acid ethyl ester. Methods of producing monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid derivative compositions produced by the recombinant microbes. The recombinant microbes may be used to produce fragrances, flavors, pheromones, pharmaceutical agents, nutraceuticals, nutritional or dietary supplements, or precursors thereof.
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
66.
RECOMBINANT MICROBES FOR PRODUCTION OF TRANS-2 UNSATURATED FATTY ACIDS AND DERIVATIVES THEREOF
Recombinant microbes comprising a novel pathway for producing trans-2 fatty acids and derivatives thereof are provided herein. The recombinant microbes comprise (1) either a combination of a 3-hydroxy acyl-ACP thioesterase and an acyl-CoA synthetase, or a 3-hydroxy acyl-ACP:CoA transacylase; (2) a heterologous R-3-hydroxy-acyl-CoA dehydratase or a heterologous R-specific enoyl-CoA hydratase; and, optionally (3) a fatty acid derivative enzyme, such as, for example, an ester synthase, an acyl-CoA reductase, or an acyl-CoA thioesterase. Methods of producing trans-2 fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant microbes. The trans-2 fatty acids and derivatives thereof produced by the recombinant microbes may be used to produce fragrances, flavors, pheromones, pharmaceutical agents, nutraceuticals, or precursors thereof.
RRR-specific enoyl-CoA hydratase and optionally a heterologous trans-2-enoyl-CoA reductase to a recombinant microbe reduces the amount of 3-hydroxy fatty acid byproducts produced by the acyl-ACP dependent fatty acid biosynthesis pathway of the recombinant microbe. Methods of producing the fatty acid derivatives are also provided, in addition to cell cultures and fatty acid derivative compositions produced by the recombinant microbes. The fatty acid derivatives produced by the recombinant microbes may be used to produce fragrances, pheromones, pharmaceutical agents, nutraceuticals or precursors thereof.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
68.
MICROBIAL PRODUCTION OF Z3-HEXENOL, Z3-HEXENAL AND Z3-HEXENYL ACETATE
RRR-3-hydroxyhexanoyl-CoA dehydratase/e2-z3-hexenoyl-CoA isomerase; a heterologous aldehyde dehydrogenase; a heterologous acyl-CoA reductase; and an alcohol dehydrogenase for the production of z3-hexenol, z3-hexenal, and/or z3-hexenyl acetate. Methods of producing z3-hexenol, z3-hexenal, and/or z3-hexenyl acetate are also provided, in addition to cell cultures and fatty acid derivative compositions produced by the recombinant microbes.
The present disclosure provides microbial organisms having decreased production of unwanted by-products (e.g, pyruvate-, CO2—, TCA-derived by-products; acetate; ethanol; and/or, alanine) to enhance carbon flux through acetyl-CoA, which can increase production of acetyl-CoA derived compounds (e.g, 1,3-BDO, MMA, and (3R)-hydroxybutyl (3R)-hydroxybutyrate, or any other acetyl-CoA derived compounds), and products made from any of these compounds. Also provided are one or more exogenous nucleic acids encoding enzymes that can decrease production of unwanted by-products (e.g, aldehyde dehydrogenase, acetyl-CoA synthase, amino acid dehydrogenase, alanine racemase, and/or citrate synthase), and/or one or more gene attenuations occurring in genes (e.g., acetolactate synthase) that result in decreased production of unwanted by-products. Various combinations of the exogenous nucleic acids and gene deletions are also provided in the present disclosure. Methods of making and using the same, including methods for culturing cells, and for the production of the various products are also provided.
The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.
C08F 36/14 - Homopolymères ou copolymères de composés contenant un ou plusieurs radicaux aliphatiques non saturés, l'un au moins contenant plusieurs liaisons doubles carbone-carbone le radical ne contenant que deux doubles liaisons carbone-carbone conjuguées contenant des éléments autres que le carbone et l'hydrogène
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
The present disclosure relates to a non-natural flavin-dependent oxidase that does not comprise a disulfide bond and that is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. In some embodiments, the non-natural flavin-dependent oxidase comprises: (i) at least 70% sequence identity to SEQ ID NO:3; and (ii) substitutions at amino acid positions Q275, C285, V323, E370, V372, N400, D404, and T438, wherein the amino acid positions correspond to SEQ ID NO:3. The present disclosure further relates to a polynucleotide, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are a composition omprising the non-natural flavin-dependent oxidase; an isolated non-natural flavin-dependent oxidase and methods of making the same; a cell extract or cell culture medium comprising the non-natural flavin-dependent oxidase; and methods of making a cannabinoid.
12345678910111212-Gly, wherein each X is any amino acid; and wherein the FAD cofactor is covalently attached to the Cys residue, wherein the flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid, and wherein the flavin-dependent oxidase is a bacterial protein or a fungal protein.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine
A61K 31/352 - Composés hétérocycliques ayant l'oxygène comme seul hétéro-atome d'un cycle, p. ex. fungichromine ayant des cycles à six chaînons avec un oxygène comme seul hétéro-atome d'un cycle condensés avec des carbocycles, p. ex. cannabinols, méthanthéline
Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
A process for synthesizing (R)-3 -hy droxybutyl (R)-3 -hy droxybutanoate from ethyl (R)-3-hydroxybutanoate and (R)-1,3-butanediol, as well a process for synthesizing (R)-3-hy droxybutyl (R)-3-hy droxybutanoate from (R)-3-hydroxybutyric acid and (R)-1,3-butanediol. Also provided are processes for isolating (R)-3-hy droxybutyl (R)-3-hy droxybutanoate, including from a fermentation broth.
C07C 67/08 - Préparation d'esters d'acides carboxyliques par réaction d'acides carboxyliques ou d'anhydrides symétriques avec le groupe hydroxyle ou O-métal de composés organiques
C07C 67/54 - SéparationPurificationStabilisationEmploi d'additifs par modification de l'état physique, p. ex. par cristallisation par distillation
The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for ex pressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
C12N 9/18 - Hydrolases agissant sur les esters d'acides carboxyliques
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
78.
ENGINEERED ENZYMES AND METHODS OF MAKING AND USING
The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semialdehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.
The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12P 17/04 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à cinq chaînons, p. ex. griséofulvine
B01D 3/00 - Distillation ou procédés d'échange apparentés dans lesquels des liquides sont en contact avec des milieux gazeux, p. ex. extraction
The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine
81.
MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING 3-HYDROXYISOBUTYRATE OR METHACRYLIC ACID RELATED THERETO
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.
C08F 120/06 - Acide acryliqueAcide méthacryliqueLeurs sels métalliques ou leurs sels d'ammonium
C08G 63/664 - Polyesters contenant de l'oxygène sous forme de groupes éther dérivés d'acides hydroxycarboxyliques
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.
The disclosure provides polypeptides and encoding nucleic acids of engineered aldehyde dehydrogenases. The disclosure also provides cells expressing an engineered form of the aldehyde dehydrogenase. The disclosure further provides methods for producing a bioderived compound, such as 3 -hydroxybutyraldehyde, 1,3 -butanediol, 4- hydroxybutyraldehyde, 1,4-butanediol, comprising culturing cells expressing an engineered aldehyde dehydrogenase.
The disclosure provides polypeptides and encoding nucleic acids of engineered aldehyde dehydrogenases. The disclosure also provides cells expressing an engineered form of the aldehyde dehydrogenase. The disclosure further provides methods for producing a bioderived compound, such as 3-hydroxybutyraldehyde, 1,3-butanediol, 4-hydroxybutyraldehyde, 1,4-butanediol, comprising culturing cells expressing an engineered aldehyde dehydrogenase.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
85.
METHOD FOR THE PROCESSING AND TRANSPORT OF HEXANE-1,6-DIAMINE OR PENTANE-1,5-DIAMINE
The invention relates to a method for the processing and transport of hexane-1,6-diamine or pentane-1,5-diamine comprising the steps of: a) Providing a first composition including 60 to 98 wt.-% water and 2 to 40 wt.-% hexane-1,6-diamine or pentane-1,5-diamine, b) partially removing water from the first composition by distillation at a first production location, whereby a second composition is generated, said second composition including the hexane-1,6-diamine or pentane-1,5-diamine and 1 wt.-% to 35 wt.-% water, and c) transporting said second composition during a span of time t from the first production location to a second production location, wherein the span of time t including times for optional temporary storage is at least 6 h.
The invention relates to a method for obtaining an amine from a composition by means of distillation and a composition obtainable by that method. The invention also relates to a method for the production of an isocyanate from the composition obtainable.
22 groups per molecule, each of them bound to a primary or secondary carbon atom, b) removing water at least partially from said first composition by distillation at a first production location in a distillation apparatus comprising at least two distillation columns operating at different head pressures, thus generating a second composition comprising the diamine and ≤ 55 wt.-% water, wherein the vapours emerging from the distillation column operating at the higher head pressure are used to evaporate the liquid in the bottoms and/or the feed of a distillation column operating at the lower head pressure at least partially, c) transporting said second composition during a span of time t from the first production location to a second production location, wherein the span of time t including times for optional temporary storage is at least 6 h.
C07C 211/36 - Composés contenant des groupes amino liés à un squelette carboné ayant des groupes amino liés à des atomes de carbone de cycles autres que des cycles aromatiques à six chaînons d'un squelette carboné saturé contenant au moins deux groupes amino liés au squelette carboné
C07C 211/27 - Composés contenant des groupes amino liés à un squelette carboné ayant des groupes amino liés à des atomes de carbone acycliques d'un squelette carboné non saturé contenant au moins un cycle aromatique à six chaînons ayant des groupes amino reliés au cycle aromatique à six chaînons par l'intermédiaire de chaînes carbonées saturées
C07C 211/18 - Composés contenant des groupes amino liés à un squelette carboné ayant des groupes amino liés à des atomes de carbone acycliques d'un squelette carboné saturé contenant des cycles autres que des cycles aromatiques à six chaînons contenant au moins deux groupes amino liés au squelette carboné
C07C 265/14 - Dérivés d'acide isocyanique contenant au moins deux groupes isocyanate liés au même squelette carboné
C08G 69/26 - Polyamides dérivés, soit des acides amino-carboxyliques, soit de polyamines et d'acides polycarboxyliques dérivés de polyamines et d'acides polycarboxyliques
88.
FLAVIN-DEPENDENT OXIDASES HAVING CANNABINOID SYNTHASE ACTIVITY
The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; methods of making cannabinoids; and compositions comprising a cannabinoid and/or the non-natural flavin-dependent oxidase.
The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; methods of making cannabinoids; and compositions comprising a cannabinoid and/or the non-natural flavin-dependent oxidase.
The disclosure provides polypeptides and encoding nucleic acids of engineered formate dehydrogenases. The disclosure also provides cells expressing an engineered formate dehydrogenase. The disclosure further provides methods for producing a bioderived compound comprising culturing cells expressing an engineered formate dehydrogenase. Where the engineered formate dehydrogenase is capable of catalyzing a conversion.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
17 - Produits en caoutchouc ou en matières plastiques; matières à calfeutrer et à isoler
18 - Cuir et imitations du cuir
22 - Cordes; filets; tentes, auvents, voiles et sacs; matières de rembourrage
23 - Fils à usage textile
24 - Tissus et produits textiles
25 - Vêtements; chaussures; chapellerie
Produits et services
Polymer compositions used in the manufacture of commercial,
industrial and domestic goods; polymer precursors used in
the manufacture of commercial, industrial and domestic
goods; polymers, biopolymers, polymeric additives and
biopolymeric additives for use in the manufacture of
industrial products and consumer and household products;
chemicals for use in industry; chemicals used in the
manufacture of fabric or textiles; and polyamide. Resins in bars, blocks, pellets, rods, sheets and tubes for
general industrial use; resins in extruded form for general
industrial use; and semi-processed synthetic resins. Bio-nylon sold as a component of tote bags, all-purpose
sports bags, all-purpose carrying bags, carry-on bags,
weekend bags, sack packs, namely, drawstring bags used as
backpacks, beach bags, toiletry bags sold empty, cosmetic
bags sold empty, shopping bags, wheeled shopping bags,
reusable shopping bags, sling bags for carrying infants,
knitted bags not of precious metals, travel bags, school
book bags, duffle bags, wheeled duffle bags, messenger bags,
diaper bags, gym bags, purses, handbags, cross-body bags,
drawstring bags, shoulder bags, waist bags, wristlet bags,
backpacks, rucksacks, wallets, not of precious metal,
briefcases, attaché cases, clutch bags, hip bags, umbrellas,
credit card cases, business card cases, key cases, pocket
wallets, haversacks, suitcases, daypacks, suit bags, garment
bags for travel, and luggage. Textile fibers; yarn fibers; synthetic fibers for textile
use; raw textile fibers; woven fibers of bio-nylon for
textile use; non-woven polymeric fibers for conversion into
a variety of industrial and consumer goods, namely,
non-woven polymeric fibers for textile use and industrial
use; woven polymeric fibers for conversion into a variety of
industrial and consumer goods, namely woven polymeric fibers
for textile use and industrial use; and synthetic fibers and
filaments for use in the manufacture of fabrics, textiles,
yarns, and carpets. Thread; yarn; synthetic thread; synthetic yarn; thread for
textile use; yarn for textile use; synthetic fiber thread
and yarn; and yarn and thread for textile purposes. Bio-nylon fabric for use as a textile in the manufacture of
clothing; textile fabrics for use in the manufacture of
garments, bags, jackets, gloves, and apparel; fabrics made
of bio-nylon for textile use; fabrics for textile use. Bio-nylon sold as a component of clothing, headwear,
footwear and outerwear for men, women and children, namely,
shirts, t-shirts, sweat shirts, sweat pants, dresses,
eveningwear, blouses, neckties, sweaters, cardigans,
jerseys, overalls, suits, skirts, shorts, trousers, pants,
yoga pants, jeans, bodysuits, leggings, leotards, tights,
hosiery, swimwear, bathing trunks, bathing suits, pajamas,
nightwear, robes, bras, underwear, panties, girdles, thermal
underwear, socks, base layer tops and bottoms, coats,
raincoats, jackets, vests, shawls, gloves, slippers, shoes,
sneakers, athletic footwear, overshoes, sandals, boots, hats
and caps being headwear, headbands being clothing,
suspenders, scarves, knee warmers, neck tubes, hand warmers,
gaiters, and clothing belts; and finished textile linings
for garments.
92.
ENGINEERED CELLS FOR PRODUCTION OF CANNABINOIDS AND OTHER MALONYL-CoA-DERIVED PRODUCTS
The invention relates to engineered microorganisms (e.g., E. coli) and associated improvements for increasing the production cannabinoids (e.g. CBGA) or precursors or derivatives thereof.
C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
93.
Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid
The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.
C12P 17/10 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'azote comme unique hétéro-atome du cycle
C12N 1/38 - Stimulation chimique de la croissance ou de l'activité par addition de composés chimiques qui ne sont pas des facteurs essentiels de croissanceStimulation de la croissance par élimination d'un composé chimique
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
C12P 17/12 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'azote comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemicals for use in the manufacture of cosmetics,
nutraceuticals, plastics, polymers, solvents, rubber,
fiber-based products, fabrics, textiles, apparel, automotive
parts, electronics, agricultural products, cleaning
products, perfumes, lubricants, fuels, home care products,
personal and beauty care products, and dietary and
nutritional supplements and beverages; polymer precursors
and polymer compositions used in the manufacture of
commercial, industrial and domestic goods; chemicals for use
in industry. Providing technical information in the field of chemical
manufacturing; biomanufacturing, namely, manufacturing of
chemicals and bio-nylon using biological organisms in the
manufacturing process. Scientific research and engineering in the fields of
chemicals, organisms, and bioprocesses; consulting services
in the field of design and development of bioengineering
processes; consulting services in the fields of
bioengineering and biotechnology; research and development
in the fields of bioengineering and biotechnology; product
development consultation about products derived from plants
or other renewable or maintainable resources; research and
development in the field of bio-based chemicals and
plant-derived products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemicals for use in the manufacture of cosmetics,
nutraceuticals, plastics, polymers, solvents, rubber,
fiber-based products, fabrics, textiles, apparel, automotive
parts, electronics, agricultural products, cleaning
products, perfumes, lubricants, fuels, home care products,
personal and beauty care products, and dietary and
nutritional supplements and beverages; polymer precursors
and polymer compositions used in the manufacture of
commercial, industrial and domestic goods; chemicals for use
in industry. Providing technical information in the field of chemical
manufacturing; biomanufacturing, namely, manufacturing of
chemicals and bio-nylon using biological organisms in the
manufacturing process. Scientific research and engineering in the fields of
chemicals, organisms, and bioprocesses; consulting services
in the field of design and development of bioengineering
processes; consulting services in the fields of
bioengineering and biotechnology; research and development
in the fields of bioengineering and biotechnology; product
development consultation about products derived from plants
or other renewable or sustainable resources; research and
development in the field of bio-based chemicals and
plant-derived products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemicals for use in the manufacture of cosmetics,
nutraceuticals, plastics, polymers, solvents, rubber,
fiber-based products, fabrics, textiles, apparel, automotive
parts, electronics, agricultural products, cleaning
products, perfumes, lubricants, fuels, home care products,
personal and beauty care products, and dietary and
nutritional supplements and beverages; polymer precursors
and polymer compositions used in the manufacture of
commercial, industrial and domestic goods; chemicals for use
in industry. Providing technical information in the field of chemical
manufacturing; biomanufacturing, namely, manufacturing of
chemicals and bio-nylon using biological organisms in the
manufacturing process. Scientific research and engineering in the fields of
chemicals, organisms, and bioprocesses; consulting services
in the field of design and development of bioengineering
processes; consulting services in the fields of
bioengineering and biotechnology; research and development
in the fields of bioengineering and biotechnology; product
development consultation about products derived from plants
or other renewable or sustainable resources; research and
development in the field of bio-based chemicals and
plant-derived products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Chemicals for use in the manufacture of cosmetics,
nutraceuticals, plastics, polymers, solvents, rubber,
fiber-based products, apparel, automotive parts and
electronics; industrial solvents; polymer precursors used in
the manufacture of commercial, industrial and domestic
goods.
98.
Microorganisms and methods for increasing co-factors
The present disclosure provides microbial organisms having increased availability of co-factors, such as NADPH, for increasing production of various products, including 1,3-BDO, MMA, (3R)-hydroxybutyl (3R)-hydroxybutyrate, amino acids, 3HB-CoA, adipate, caprolactam, 6-ACA, HMD A, or MAA, and products made from any of these. Also provided are one or more exogenous nucleic acids encoding an enzyme expressed in a sufficient amount to increase availability of NADPH, where the exogenous nucleic acid includes one or more of ATP-NADH kinase, pntAB, nadK, and gapN. Also provided are one or more gene attenuations occurring in genes, such as NDH-2, that result in an increased ratio of NADPH to NADH. Various combinations of the exogenous nucleic acids and gene deletions are also provided in the present disclosure. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of the various products.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
99.
1,3-BUTYLENE GLYCOL COMPOSITIONS AND METHODS OF USE THEREOF
The disclosure provides embodiments directed to a composition comprising a 1,3 -butylene glycol (1,3-BG; such as but not limited to, bioBG) and one or more fragrance ingredients, which form a fragrance component of the composition. 1,3-BG demonstrates exemplary qualities and characteristics of being natural, sustainable, and substantially non-odorous, for use in fragrance compositions. Methods of using or applying such 1,3-BG fragrance compositions provide a cost- efficient, sustainable composition, since 1,3-BG does not mask or alter the fragrance notes of the fragrance ingredients allowing for the use of smaller quantities of fragrance ingredients. The 1,3-BG fragrance compositions of the disclosure also improve fragrance performance and reduces greenhouse gases, while avoiding any irritation, sensitization, or allergic reaction in humans.
The present disclosure provides a polynucleotide comprising: (a) a nucleic acid sequence encoding an olivetol synthase (OLS); and (b) a heterologous regulatory element operably linked to the nucleic acid sequence. The present disclosure further relates to an engineered cell comprising an olivetol synthase (OLS). Also provided are a cell extract or cell culture medium or a composition comprising 3,5,7- trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof, and a method of making 3,5,7-trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof. Also provided is a cannabinoid produced by the engineered cell, isolated from the cell extract or cell culture medium, and/or made by the method described herein. The present disclosure also provides a non-natural olivetol synthase (OLS).
