Disclosed are methods for isolating an analyte from a sample. In some aspects, the methods are for selectively isolating a microbial cell analyte, such as a nucleic acid, from a sample containing or suspected of containing mammalian cells. The selective isolation method includes selective lysis of the mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analyte. The released analyte is then eluted from the filter. In other aspects, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes may be analyzed using a suitable assay depending on the type of analyte molecule. Also disclosed are fluidic systems and lysis solutions that may be used for isolating an analyte according to the disclosed methods.
C12N 9/36 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons bêta-1, 4 de l'acide N-acétylmuramique avec l'acétylamino-2 déoxy-2-D-glucose, p. ex. lysozyme
3.
DETECTING BABESIA SPECIES NUCLEIC ACID IN A SAMPLE
A combination of at least two oligomers is for determining the presence or absence of Babesia in a sample. The combination includes first and second amplification oligomers for amplifying a target region of Babesia target nucleic acid. The first amplification oligomer includes a first target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:97 and includes SEQ ID NO: 101 or its analogue. The second amplification oligomer includes a second target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length. The second amplification oligomer can also be contained in SEQ ID NO:68 and include SEQ ID NO:85 or its analogue, or contained in SEQ ID NO:70 and include SEQ ID NO:48, or include SEQ ID NO:84.
C12Q 1/6893 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les protozoaires
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
A cam-driven actuator for actuating valves of a microfluidic cartridge includes a plunger associated with each valve, an actuating ball associated with each plunger and in contact with the associated plunger, and a cam including a first surface and a cam recess. Each actuating ball is in contact with the cam and the cam is movable with respect to each actuating ball between (i) an engaged position at which the actuating ball is in contact with the first surface and the associated plunger is in an engaged position to close the associated valve, and (ii) a disengaged position at which the actuating ball is disposed within the cam recess and the associated plunger is in a disengaged position to open the associated valve.
A receptacle clamping mechanism of an instrument includes a carriage configured to move between a first location and a second location of the instrument. The carriage includes one or more support members configured to removably support a receptacle therebetween and a pair of opposed support pads configured to apply a clamping force to a receptacle supported by the carriage as the carriage moves from the first location to the second location and release the clamping force from the receptacle as the carriage moves from the second location to the first location. The clamping mechanism further includes a sensing system configured to determine whether a receptacle is supported by the carriage.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
7.
APPARATUS FOR APPLYING THERMAL ENERGY TO A RECEPTACLE AND DETECTING AN EMISSION SIGNAL FROM THE RECEPTACLE
A method for determining the presence of a target nucleic acid in a sample includes transporting multiple receptacles to each of a plurality of corresponding receptacle wells of a thermally-conductive receptacle holder, each of the receptacles containing a reaction mixture, wherein the receptacle holder is in thermal communication with a support, and wherein the support is in thermal communication with a heat sink, pre-heating the heat sink to a temperature above ambient temperature, cycling the temperature of the receptacle holder, establishing optical communication between each of the receptacle wells and an excitation signal source and an emission signal detector, and determining whether an emission signal is emitted from any of the receptacles as an indication of the presence of a target nucleic acid.
A method for analyzing samples is performed within a housing. Each sample is contacted in a separate receptacle with a magnetically-responsive solid support for binding and immobilizing a nucleic acid of interest that may be present in one or more of the samples. Each sample is processed to isolate and purify any nucleic acid of interest that is present. An elution buffer is provided to each separate receptacle, thereby forming an eluate containing the nucleic acid of interest, if present in the associated sample. A unit-dose reagent for performing a nucleic acid amplification reaction is reconstituted for each sample, and the reconstituted reagent is combined with the eluate in one of a plurality of processing vials. Each of the processing vials containing reconstituted reagent and eluate is closed with a cap and transferred to an incubator and the contents are subjected to nucleic acid amplification conditions.
A container comprises a top wall and a fluid vessel depending from a top wall aperture formed in the top wall to a bottom end of the fluid vessel. A first wall segment depends from a first perimeter segment of the top wall and extends from the first perimeter segment to a bottom edge of the first wall segment and at least partially surrounds the fluid vessel. At least a portion of the bottom edge of the first wall segment is disposed below the bottom end of the fluid vessel and includes an alignment notch extending upwardly from the bottom edge of the first wall segment. A top wall fluid retainer barrier projects above a top surface of the top wall and extends continuously around the top wall aperture and is spaced from a perimeter of the top wall aperture.
After disposable pipette tips are used by an automated pipettor, they are released by the pipettor and fall into a waste container. When the waste container is removed to be emptied, the pipette tips are temporarily sequestered in a pipette tip holding station so that the automated pipettor may operate uninterrupted. After the waste container is replaced, the sequestered pipette tips are released by the holding station into the waste container.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
An apparatus includes vertically spaced holding shelves, each including first and second shelf portions separated by an open area. A carrier configured to hold consumables is longer than the open area so that ends of the carrier can be supported on the first and second shelf portions. A transporter includes a support chassis laterally spaced from the holding shelves, a lift platform for carrying the carrier and having a length that is less than the length of the open area, a scissors actuator connecting the lift platform to the support chassis and configured to translate the lift platform laterally between a first position aligned with the support chassis and a second position laterally displaced from the support chassis and aligned with the open area of one of the holding shelves, and a transport elevator coupled to the support chassis for moving the support chassis in a vertical direction between the plurality of holding shelves.
After disposable pipette tips are used by an automated pipettor, they are released by the pipettor and fall into a waste container. When the waste container is removed to be emptied, the pipette tips are temporarily sequestered in a pipette tip holding station so that the automated pipettor may operate uninterrupted. After the waste container is replaced, the sequestered pipette tips are released by the holding station into the waste container.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
After disposable pipette tips are used by an automated pipettor, they are released by the pipettor and fall into a waste container. When the waste container is removed to be emptied, the pipette tips are temporarily sequestered in a pipette tip holding station so that the automated pipettor may operate uninterrupted. After the waste container is replaced, the sequestered pipette tips are released by the holding station into the waste container.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
A system for specifying user-defined assay parameters of a user-defined assay protocol for processing a sample includes a first graphical user interface for defining an analyte extraction parameter for an extraction process to extract a targeted analyte from the sample, a second graphical user interface for defining a target parameter specifying one or more channels of a multi-channel signal detector for detecting the targeted analyte, a third graphical user interface for defining parameters of a thermal profile specifying thermal conditions to which a reaction mixture is to be exposed to amplify the targeted analyte, and system-defined assay protocols pre-programmed into storage media and performed in accordance with system-defined assay parameters that are not changeable by a user and wherein at least one of the system-defined assay parameters is combined with the user-defined assay parameters to form the user-defined assay protocol.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Disclosed are methods for diagnosing Bacterial Vaginosis in a subject comprising performing an assay for the detection of any one or more of Lactobacillus sp., Atopobium vaginae, and Gardneralla vaginalis in a subject sample. Also disclosed are compositions and methods for detecting Lactobacillus sp., Atopobium vaginae, and/or Gardneralla vaginalis nucleic acid in a sample.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
Populations of target capture probes are provided that are useful for nucleic acid separation and purification. The probes of the population comprise a first region that is at least about 12 residues in length and comprises a poly(r) sequence comprising (i) a randomized sequence comprising G and A nucleotides, or (ii) a non-randomized repeating (A and G) sequence; and a second region comprising a first specific binding partner (SBP), wherein the SBP is capable of specifically binding a second specific binding partner (SBP2). Related combinations, methods, uses, kits, and reaction mixtures are also provided.
Performing multiple nucleic acid amplification assays in an automated analyzer comprises loading the analyzer with a sample-containing receptacles, producing a purified form of a first sample to isolate a first analyte, producing a purified form of a second sample to isolate a second analyte, forming first and second amplification reaction mixtures with the purified first and second samples, the first mixture containing first amplification oligomers for amplifying a first region of the first analyte and the second mixture containing second amplification oligomers for amplifying a second region of the second analyte, exposing the second mixture to thermal conditions for amplifying the second region, exposing the first mixture to thermal conditions for amplifying the first region, determining the presence or absence of the second analyte in the second mixture, and determining the presence or absence of the first analyte in the first mixture.
A method of processing a sample in a receptacle having one or more assay order states can include automatically determining assay order states of the discrete areas and performing an assay on the sample, using the automatic sample processing instruments, based on the determined assay order states of the discrete areas. Each discrete area has a known association with a different assay. Each discrete area can be altered from a first assay order state to a second assay order state. The method includes a step of automatically determining assay order states of the plurality of discrete areas by determining whether each discrete area has been altered from the first assay order state to the second assay order state.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 10/65 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients stockées sur des supports d’enregistrement portables, p. ex. des cartes à puce, des étiquettes d’identification radio-fréquence [RFID] ou des CD
19.
LABELS, RECEPTACLES, SYSTEMS, AND METHODS FOR AUTOMATIC SAMPLE PROCESSING
A sample receptacle can be used for ordering assays to be performed by an automatic sample processing instrument. The sample receptacle can include a body defining a chamber for containing a sample and a label. The label can include discrete areas configured to be altered from a first assay order state to a second assay order state. Each discrete area has a known association with a different assay. The label can also include assay-identifying indicia indicating the respective assay associated with a respective discrete area.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 10/65 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients stockées sur des supports d’enregistrement portables, p. ex. des cartes à puce, des étiquettes d’identification radio-fréquence [RFID] ou des CD
A computer-implemented method for determining the amount of an analyte in a sample comprises associating a nucleic acid amplification assay defined at least partly by user-defined assay parameters to the sample. The assay is performed by dissolving a unit-dose reagent with a solvent, wherein the solvent includes one or more amplification oligomers adapted to amplify a region of the analyte and the reagent does not include an amplification oligomer for performing the assay. A reaction mixture, including the dissolved reagent and the sample, is exposed to a temperature condition and measurements of fluorescence indicative of an amount of amplification products formed during the exposing are collected. The measurements are assessed with a computer using data analysis parameters provided by the user to compute results indicative of the amount of the analyte in the sample.
A system comprising an analyzer for performing nucleic acid amplification assays includes a controller configured to send instructions to purify a first sample by immobilizing a first analyte, purify a second sample by immobilizing a second analyte, form a first reaction mixture by combining a dissolved second reagent with the purified second sample, wherein a second solvent for dissolving the second reagent contains second amplification oligomers for amplifying the second analyte and the second reagent contains a polymerase but does not contain amplification oligomers for amplifying the second analyte, and form a second reaction mixture by combining a dissolved first reagent with the purified first sample, wherein the first reagent contains a polymerase and first amplification oligomers for amplifying the first analyte and a first solvent for dissolving the first reagent does not contain an amplification oligomer or a polymerase for amplifying the first analyte.
Analyzing samples using an automated analyzer comprises retaining a first container containing a first solvent lacking an amplification oligomer at a first location of the analyzer, retaining a second container having a different structure than the first container and supporting solvent-containing vials at a second location of the analyzer, the solvent in each vial including an amplification oligomer, combining a first non-liquid reagent including at least one amplification oligomer with the first solvent and a first sample to form a first reaction mixture, combining a second non-liquid reagent lacking an amplification oligomer with the solvent included in a vial of the second container and a second sample to form a second reaction mixture, performing first and second amplification reactions with the first and second reaction mixtures, and determining the presence or absence of one or more analytes in the first and second reaction mixtures.
This disclosure provides oligomers, compositions, and kits for detecting and quantifying Hepatitis B virus (HBV), including different genotypes and variants thereof, and related methods and uses. In some embodiments, oligomers target the P and/or S open reading frames of HBV and are configured to provide substantially equivalent quantification of different genotypes and variants of HBV.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Disclosed are methods utilizing specific amplification of Candida sp. target nucleic acid for detecting the presence or absence of Candida sp. in a sample. Also disclosed are corresponding oligomers, including amplification oligomers, capture probes and detection probes, and combinations thereof, as well as corresponding reaction mixtures and kits.
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
25.
Method of Quantifying Polynucleotide Analytes from Dried Samples
Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.
C12Q 1/6865 - Amplification à base de promoteurs, p. ex. amplification de séquence d’acide nucléique [NASBA], système de réplication de séquence auto-entretenue [3SR] ou d’amplification à base de transcription [TAS]
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
26.
EVAPORATION-LIMITING INSERTS FOR REAGENT CONTAINERS AND RELATED METHODS OF USE
An insert for a liquid-holding container includes a body comprising a wall, open first and second ends, and a lumen extending from the open first end to the open second end. A plurality of first openings are formed in the wall, each of the first openings defining an area, and one or more second openings are formed in the wall, each of the one or more second openings defining an area that is greater than the area defined by any of the first openings. The first openings are arranged in a first row of at least three first openings in axial alignment and a second row of at least three first openings in axial alignment. At least one of the one or more second openings is situated closer to the first end than any of the first openings, and each of the first and second openings is sized to permit the passage of a liquid.
B01F 23/00 - Mélange, p. ex. dispersion ou émulsion, selon les phases à mélanger
B01F 29/30 - Mélange du contenu d'emballages ou de récipients individuels, p. ex. en faisant tourner des boîtes de conserve ou des bouteilles
B01F 31/22 - Mélange du contenu de récipients indépendants, p. ex. de tubes à essai avec des moyens de support se déplaçant dans un plan horizontal, p. ex. en décrivant un trajet orbital pour déplacer les récipients autour d'un axe qui coupe l'axe du récipient selon un certain angle
B01F 35/511 - Récipients de mélange munis de doublures, p. ex. de doublures résistantes à l'usure ou flexibles
B01F 35/53 - Récipients de mélange caractérisés par la configuration de l'intérieur, p. ex. chicanes pour faciliter le mélange des composants
B01F 101/23 - Mélange d'échantillons de laboratoire, p. ex. en vue d'analyser ou de tester les propriétés des matières
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
27.
Compositions and Methods for Detecting Nucleic Acids of Epstein-Barr Virus
Disclosed are compositions, methods, and kits that can be used to Epstein-Barr virus (EBV) in a sample undergoing testing. Nucleic acids of EBV can be isolated, amplified and detected with specificity, and without interference from non-EBV organisms. In some embodiments, nucleic acids used for amplification are isolated from human blood, or blood products. Nucleic acid isolation, amplification and detection steps can all be carried out using an automated instrument.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. A biological sample is combined with an alkaline composition that lyses cells and denatures DNA to create a first liquid composition. The first liquid composition is then mixed with a buffer, a detergent, and a solid support that captures DNA to create a second liquid composition. The buffer, detergent, and solid support can be delivered as components of a single reagent. Captured DNA strands can be used as templates in subsequently performed nucleic acid amplification and detection reactions with improved sensitivity.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
29.
SYSTEM FOR READING MACHINE-READABLE LABELS ON SAMPLE RECEPTACLES
A system including a housing, a sample rack for holding a plurality of sample receptacles, and a reader for reading two-dimensional machine-readable labels associated with the plurality of sample receptacles as the sample rack moves between first and second positions within the housing. The system further includes a processing and control unit adapted to decode the two-dimensional machine-readable labels read by the reader and to associate each of the decoded two-dimensional machine-readable labels with an associated sample receptacle based on a measured position of the sample rack when the two-dimensional machine-readable label was read.
G06K 7/10 - Méthodes ou dispositions pour la lecture de supports d'enregistrement par radiation électromagnétique, p. ex. lecture optiqueMéthodes ou dispositions pour la lecture de supports d'enregistrement par radiation corpusculaire
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
G06K 7/14 - Méthodes ou dispositions pour la lecture de supports d'enregistrement par radiation électromagnétique, p. ex. lecture optiqueMéthodes ou dispositions pour la lecture de supports d'enregistrement par radiation corpusculaire utilisant la lumière sans sélection des longueurs d'onde, p. ex. lecture de la lumière blanche réfléchie
G06K 19/06 - Supports d'enregistrement pour utilisation avec des machines et avec au moins une partie prévue pour supporter des marques numériques caractérisés par le genre de marque numérique, p. ex. forme, nature, code
30.
RECEPTACLE TRANSPORT SYSTEM FOR AN ANALYTICAL SYSTEM
A receptacle delivery system for an instrument of a plurality of instruments comprises a carriage supporting a puck. The carriage is configured to move with the puck from a first location to a second location within the instrument. The first location is a location where a receptacle supported by a carrier is positioned to be transferred to the puck supported by the carriage, and the second location being a location where the receptacle seated in the puck is positioned so that fluid from the receptacle can be drawn into a tip associated with a fluid extraction device of the instrument.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
The present disclosure relates to compositions and methods, and related systems, products and kits, for performing temperature-dependent multiplex invasive cleavage assays, where a plurality of target nucleic acids are detected and distinguished from each other in a procedure using only a single fluorescent moiety as the reporter, and single channel fluorescence detection. In some embodiments, at least one of the plurality of target nucleic acids is an amplified nucleic acid, where progress in a thermal cycling amplification reaction is monitored as a function of time (i.e., real-time amplification).
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Specimen collection kit comprised of a solution for
collecting and transporting a sample for scientific research
purposes. Specimen collection kit comprised of a swab and solution for
collecting and transporting a sample for medical purposes. Scientific research specimen collection devices in the
nature of a swab and collection tube for the collection of
respiratory specimens. Medical specimen collection devices in the nature of a
collection tube for the collection of respiratory specimens.
A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., “SNPs”) in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.
A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., “SNPs”) in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.
A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., “SNPs”) in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.
Provided herein are compositions, kits, and methods for detecting at least one of a C. difficile tcdA, tcdB, tcdC, cdtA, or cdtB nucleic acid in a sample. In some embodiments, one or more alleles of tcdC such as 117del tcdC or 184T tcdC are detected.
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
37.
LABORATORY AUTOMATED SYSTEMS FOR TRANSPORTING SAMPLE RECEPTACLE CARRIERS
An apparatus is directed to transporting a sample receptacle carrier coupled to a sample containing receptacle. The apparatus includes a movable dock to support the sample receptacle carrier. The apparatus includes one or more magnets. Each magnet is coupled to the movable dock to magnetically couple with a magnetic portion of the sample receptacle carrier. The one or more magnets secure the sample receptacle carrier to the movable dock.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 1/14 - Dispositifs d'aspiration, p. ex. pompesDispositifs d'éjection
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
38.
COMPOSITIONS FOR DETECTING BORDETELLA PARAPERTUSSIS NUCLEIC ACID
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Bordetella pertussis and Bordetella parapertussis nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
39.
COMPOSITIONS FOR DETECTING BORDETELLA PERTUSSIS NUCLEIC ACID
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Bordetella pertussis and Bordetella parapertussis nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
40.
AUTOMATED PROCESSING OF SAMPLES CARRIED IN SAMPLE CONTAINERS AND GROUPING SAMPLE CONTAINERS ACCORDING TO ASSAYS TO BE PERFORMED ON SAMPLES CONTAINED THEREIN
A system for processing samples includes a sample database storing identification information correlated with one or more open assays associated with each sample. A conveyance transports sample containers, an input module holds sample containers, and a transfer robot transfers containers from the input module to the conveyance. An input scanner detects the machine-readable identification information on each sample container. At least one analyzer is configured to perform one or more functional assays on sample extracted from a sample container. A system controller is programmed to cause the transfer robot to transfer containers from the input module to the conveyance before scanning the machine-readable identification information and before identifying the one or more open assays associated with the sample container. The controller activates the input scanner to scan the machine-readable identification information as the sample container passes the input scanner and to access the sample database and identify one or more open assays for each sample container based on the identification information detected by the input scanner.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
41.
JIG FOR POSITIONING AND SECURING A FIRST CONSOLE WITH RESPECT TO A SECOND CONSOLE
A jig assembly for connecting first and second consoles with predefined relative positioning includes first and second positioning pins attached or attachable to the first console and a positioning connection bracket attached or attachable to the second console and including a longitudinal span and first and second transverse legs including first and second pin recesses at respective free ends thereof. The first and second pin recesses receive the first and second positioning pins, respectively, when the first and second consoles are moved laterally toward each other. The first and second transverse legs may be attached to the first console with a first and second leg attachment brackets, respectively. The first and second positing pins and the first and second leg attachment brackets may be fixed with respect to the first console by attachment to a mounting rail that is fixed to the first console.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
Disclosed are methods for selectively isolating a microbial cell analyte (e.g., a nucleic acid) from a sample containing or suspected of containing mammalian cells. The selective isolation method includes selective lysis of the mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by release of the retained microbial cells from the filter. The microbial cells are then collected from the filter using reverse-flow elution or aspiration, and the collected microbial cells are lysed to release the analyte from the intact cells. The isolated analytes may be analyzed using a suitable assay depending on the type of analyte molecule.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
43.
AUTOMATED FLUID SAMPLE HANDLING SYSTEMS AND METHODS
Systems and corresponding methods transfer liquid sample from a first container having a cap with a sample collection swab coupled thereto to a second container having a cap without such sample collection swap coupled thereto. Other systems and corresponding methods remove a cap having no sample collection swab coupled thereto from a first container and remove a cap having a sample collection swab coupled thereto from a second container containing a liquid sample. The cap with the sample collection swab coupled thereto is then secured to the first container and the cap having no sample collection swap coupled thereto is then secured to the second container.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
A tray for transferring a plurality of solid reagents to a multi-well cartridge, the tray including a top surface having a plurality of depressions formed therein, each depression being configured to receive and hold a single one of the solid reagents, and a barrier wall projecting above and substantially surrounding the top surface, where the barrier wall is discontinuous at a first end of the tray.
A sample receptacle transporting carrier includes a base defining a recess for receiving a bottom end of the sample receptacle. The carrier also includes a plurality of resilient wire fingers affixed to and extending upward from the annular support. The fingers are configured to retain the bottom end of the sample receptacle within the recess of the base and to maintain the sample receptacle in an upright orientation. Each of the wire fingers includes a first segment adjacent to the base, a linear second segment joined to the first segment by a first angled portion, and a linear third segment joined to the second linear segment by a second angled portion. The third segment length is greater than the second segment length. The third segment of each finger is configured to contact the bottom end of the receptacle as the receptacle is inserted into the carrier. And the third segment and the vertical axis of the carrier form an angle between about 40° and about 50°.
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
B01L 9/00 - Dispositifs de supportDispositifs de serrage
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
A system for disposing of spent containers comprises a retainer cage disposed over a waste opening. The retainer cage comprises opposed, vertically-oriented first and second sides, an upper retainer bar and a lower retainer bar extending laterally from the first side of the retainer cage toward the second side, and a container gripper. The retainer bars are vertically spaced from one another and extend across a portion of the width of the retainer cage leaving a gap between the second side and terminal ends of the retainer bars, and the gap between the retainer bars and the second side is configured to permit a container to be inserted through the gap. The gripper is configured to hold the container, insert the container through the gap to a position between the first and second sides, and move to a position whereby the gripper is positioned between the vertically-spaced retainer bars and the container is located behind the retainer bars.
B65G 29/00 - Transporteurs rotatifs, p. ex. disques, bras, croisillons ou cônes rotatifs
B65G 47/84 - Roues en forme d'étoiles ou dispositifs à courroies ou chaînes sans fin, les roues ou dispositifs étant dotés d'éléments venant en prise avec les objets
G01F 23/263 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p. ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p. ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques en mesurant les variations de capacité de condensateurs
47.
METHOD AND SYSTEM FOR IMPROVING SPECIFICITY OF ANALYTE DETECTION USING REAL-TIME NUCLEIC ACID AMPLIFICATION
Methods and systems including programmed computers that can be used for improving nucleic acid analyte detection using real-time amplification and monitoring, where closely related nucleic acid sequences can be resolved from each other. In one embodiment, detection of target sequences differing from each other by a single nucleotide were resolved by applying a mathematical transformation. In another embodiment, two target sequences differing from each other by a single nucleotide were detected and resolved from each other using a labeled probe specific for only one of the two target sequences.
Kits for detecting analyte polynucleotides and an internal control in a sample. Included in the kit are an internal control polynucleotide and amplification reagents to co-amplify a first analyte polynucleotide and the internal control. Also included are first and second hybridization probes, each having a label indistinguishable from the other. The probes are respectively capable of hybridizing with a first analyte amplicon and an internal control amplicon. The first and second labels are indistinguishable homogeneous labels.
Systems and methods for loading reagent pellets into wells of a cartridge include a pellet transfer head including vacuum nozzles corresponding to the number and arrangement of the wells of the cartridge, a pellet reservoir holding reagent pellets, a vision sensor system for performing various system vision checks, and vertical and horizontal carriage assemblies to effect relative movement between the transfer head, pellet reservoir, cartridge, and vision sensor system. The pellet transfer head may include downwardly-facing pressure ports to supply gas flow into the pellet supply reservoir to fluidize the supply of pellets and a static eliminator fan to eliminate or reduce static buildup among pellets in the reservoir. A pellet is drawn from the reservoir to each vacuum nozzle, and the vision sensor system confirms that a single, unbroken and undeformed pellet is positioned at each nozzle and then confirms transfer of a single pellet to each well.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
51.
Compositions and Methods for Detecting C1orf43 Nucleic Acid
This disclosure provides oligomers, combinations of oligomers, compositions, kits, uses, and methods for detecting a C1orf43 nucleic acid, such as C1orf43 mRNA, such as human C1orf43 mRNA, in a sample.
A cam-driven actuator for actuating valves of a microfluidic cartridge includes a plunger associated with each valve, a plunger guide plate having a plunger guide hole for each plunger, an actuating ball associated with each plunger and in contact with the associated plunger, a ball guide plate having a ball guide hole for each actuating ball; and a cam including a first surface and a cam recess. Each actuating ball is in contact with the cam and the cam is movable with respect to each actuating ball between (i) an engaged position at which the actuating ball is in contact with the first surface and the associated plunger is in an engaged position to close the associated valve, and (ii) a disengaged position at which the actuating ball is disposed within the cam recess and the associated plunger is in a disengaged position to open the associated valve.
Methods, systems, reaction mixtures, kits, apparatuses, and systems for monitoring nucleic acid amplification reaction mixtures for abnormalities. Exemplary methods include determining fluorescence-based values from fluorophore-containing nucleic acid amplification reaction mixtures and determining whether those values satisfy predetermined values or indicate abnormalities.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
G01N 21/01 - Dispositions ou appareils pour faciliter la recherche optique
Methods, systems, and computer readable media for monitoring fluorescence signals from a nucleic acid amplification reaction for abnormal fluorescence detection. Exemplary methods include determining fluorescence-based values from fluorophore-containing nucleic acid amplification reaction mixtures and determining whether those values satisfy predetermined thresholds and/or ranges or otherwise indicate abnormalities.
Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Methods, systems, computer readable media of monitoring for the introduction of one or more liquids into a reaction chamber. Exemplary methods include determining fluorescencebased values from fluorophore-containing nucleic acid amplification reaction mixtures and determining whether those values satisfy predetermined thresholds and/or ranges or otherwise indicate abnormalities.
G01N 21/01 - Dispositions ou appareils pour faciliter la recherche optique
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
(1) Specimen collection kit comprised of a solution for collecting and transporting a sample for scientific research purposes.
(2) Specimen collection kit comprised of a swab and solution for collecting and transporting a sample for medical purposes.
(3) Scientific research specimen collection devices in the nature of a swab and collection tube for the collection of respiratory specimens.
(4) Medical specimen collection devices in the nature of a collection tube for the collection of respiratory specimens.
58.
LABORATORY AUTOMATED SYSTEMS FOR TRANSPORTING SAMPLE RECEPTACLE CARRIERS
A laboratory automated system includes a conveyor assembly, a dock that that is movable between first and second positions, a drive assembly for moving the dock between the first and second positons, and a diverter for diverting a sample receptacle carrier from the conveyor assembly to the dock. The dock includes a first carrier support portion having a planar surface for supporting the sample receptacle carrier as the dock moves between the first and second positions. The dock includes an upwardly extending second carrier support portion situated adjacent the first carrier support portion. The second carrier support portion has a contoured side surface. The dock includes one or more magnets fixedly contained within or disposed on the second carrier support portion to magnetically couple with a magnetic portion of the sample receptacle carrier.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 1/14 - Dispositifs d'aspiration, p. ex. pompesDispositifs d'éjection
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
An automated method for performing an amplification reaction includes the automated steps of combining a fluid sample together with one or more amplification reaction reagents in a reaction receptacle using a first automated pipettor, thereby forming a reaction mixture, transporting the reaction receptacle to a first location of a centrifuge, subjecting the reaction mixture to centrifugation, after subjecting the reaction mixture to centrifugation, removing the reaction receptacle from a second location of the centrifuge different from the first location and placing the reaction receptacle in a thermal cycler, and in the thermal cycler, subjecting the reaction mixture to one or more temperature cycles.
Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Sample receptacles are automatically transferred from first and second storage areas of a storage module to a conveyor without passing over any other sample receptacle stored in the storage module. Receptacle holding positions in the first and second storage areas are arranged in rows and columns, and sample containers are transferred row-by-row or column-by-column, starting with sample container held closest to a first side of the first or second storage area. Receptacles transferred from the first storage area are transferred across the first side to the conveyor. Receptacles transferred from the second storage area are transferred across the first side into a first receptacle transport path between the first and second storage areas, and then to a second receptacle transport path across the first storage area, and then to the conveyor. To avoid passing a receptacle over another receptacle, receptacles are first removed from intervening holding positions between a respective holding position from which the receptacle is being transferred and the first side of the first or second storage area.
B65G 1/137 - Dispositifs d'emmagasinage mécaniques avec des aménagements ou des moyens de commande automatique pour choisir les objets qui doivent être enlevés
A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., “SNPs”) in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.
Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
65.
Compositions and methods for detecting or quantifying Human Parainfluenza Virus 4
Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
66.
Method of quantifying polynucleotide analytes from dried samples
Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.
G06F 30/20 - Optimisation, vérification ou simulation de l’objet conçu
C12Q 1/6865 - Amplification à base de promoteurs, p. ex. amplification de séquence d’acide nucléique [NASBA], système de réplication de séquence auto-entretenue [3SR] ou d’amplification à base de transcription [TAS]
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
67.
COMPOSITIONS AND METHODS FOR DETECTING OR QUANTIFYING HUMAN PARAINFLUENZA VIRUS 1
Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
68.
RECEPTACLE TRANSPORT SYSTEM FOR AN ANALYTICAL SYSTEM
Providing a fluid to an instrument comprises supporting a sample receptacle on a first carrier, transporting the first carrier on a conveyor adjacent a plurality of modules including at least one instrument, stopping the first carrier at the instrument, transporting the sample receptacle to a pick-up position of the instrument, transporting the sample receptacle from the pick-up position to a pipetting station within the instrument, aspirating fluid within the sample receptacle and transferring the aspirated fluid to a reaction receptacle, after aspirating fluid from the sample receptacle, transporting the sample receptacle to the pick-up position, transporting the sample receptacle from the pick-up position to a second carrier the conveyor, in the instrument, performing an assay to determine the presence or absence of an analyte in the aspirated fluid, and transporting the second carrier on the conveyor to one or more of the plurality of modules other than the analytical instrument.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
69.
COMPOSITIONS AND METHODS FOR DETECTING GASTROINTESTINAL PARASITES
Cryptosporidium Entamoeba histolyticaGiardia lambliaCyclospora cayetanensisCyclospora cayetanensis target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.
C12Q 1/6893 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les protozoaires
70.
RECEPTACLE TRANSPORT SYSTEM FOR AN ANALYTICAL SYSTEM
Delivering a receptacle to an instrument comprises supporting a receptacle in a carriage when the carriage is positioned at a first location of the instrument, activating a sensing system coupled to the carriage to confirm that the receptacle is supported by the carriage, moving the carriage, and the receptacle supported therein, to a second location of the instrument, applying a clamping force to the receptacle as the carriage moves from the first location to the second location, at the second location, extracting at least a portion of a fluid contained in the receptacle using a fluid extraction device of the instrument, moving the carriage, and the receptacle supported therein, from the second location to the first location, and releasing the clamping force from the receptacle as the carriage moves from the second location to the first location.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
71.
Method and System For Performing a Cumulative Nucleic Acid Amplification Reaction
The present disclosure is directed to a nucleic acid amplification based method for determining the presence or absence of a target polynucleotide in a sample, wherein a portion of the amplified reaction mix is replaced with a further portion of the sample between pre-amplification steps to obtain a mix of an amplified reaction and an unamplified sample. The present disclosure thus provides a cumulative amplification reaction for highly sensitive detection of target polynucleotides. The present disclosure is also directed to a nucleic acid amplification reaction system for performing such a cumulative amplification reaction.
A mechanism for grasping a container having grooves formed on opposed sides thereof includes a chassis that rotates about a chassis axis of rotation and a gripper carriage supported for rotation with the chassis and configured for movement in a radial direction with respect to the chassis axis of rotation. The gripper carriage includes a container gripper with a first gripper element that includes a first hook; and a second gripper element that includes a second hook spaced. The first hook and the second hook are bent toward each other, and the first gripper element and the second gripper element are coupled to one another for coordinated pivoting movement toward each other or away from each other. The container gripper is configured to grasp a container by pivoting the first and second gripper elements toward each other until the respective first and second hooks each engage one of the grooves of the container.
B65G 29/00 - Transporteurs rotatifs, p. ex. disques, bras, croisillons ou cônes rotatifs
B65G 47/84 - Roues en forme d'étoiles ou dispositifs à courroies ou chaînes sans fin, les roues ou dispositifs étant dotés d'éléments venant en prise avec les objets
G01F 23/263 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p. ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p. ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques en mesurant les variations de capacité de condensateurs
A liquid container system having a liquid container, where the liquid container includes (i) an intermediate top wall, (ii) a liquid inlet tower extending above the intermediate top wall, (iii) a liquid inlet fluidly connected to the liquid inlet tower at a position above the intermediate top wall and through which the liquid container receives liquid from a liquid source, and (iv) a vacuum tower extending above the intermediate top wall and to which a pressure differential source can be fluidly connected at a position above the intermediate top wall to draw liquid into the liquid container through the liquid inlet.
F04B 49/025 - Commande d'arrêt, de démarrage, de décharge ou de ralenti par flotteurs
F04B 37/14 - Pompes spécialement adaptées aux fluides compressibles et ayant des caractéristiques pertinentes non prévues dans les groupes ou présentant un intérêt autre que celui visé par ces groupes pour utilisation particulière pour obtenir un vide élevé
F04B 45/02 - Pompes ou installations de pompage, ayant des organes de travail flexibles, spécialement adaptées pour fluides compressibles ayant des soufflets
F04B 49/03 - Commande d'arrêt, de démarrage, de décharge ou de ralenti par clapets
A liquid container system including a transfer container for receiving liquid transferred to the transfer container by a liquid transfer pump, where the transfer container includes (i) a main body, (ii) a connector shelf extending laterally from the main body and including a horizontal portion and defining a bottom wall, and (iii) a liquid transfer connector fitting extending downwardly from the bottom wall of the horizontal portion of the connector shelf and configured for fluidly connecting the transfer container to the liquid transfer pump.
F04B 49/025 - Commande d'arrêt, de démarrage, de décharge ou de ralenti par flotteurs
F04B 37/14 - Pompes spécialement adaptées aux fluides compressibles et ayant des caractéristiques pertinentes non prévues dans les groupes ou présentant un intérêt autre que celui visé par ces groupes pour utilisation particulière pour obtenir un vide élevé
F04B 45/02 - Pompes ou installations de pompage, ayant des organes de travail flexibles, spécialement adaptées pour fluides compressibles ayant des soufflets
F04B 49/03 - Commande d'arrêt, de démarrage, de décharge ou de ralenti par clapets
Disclosed are formulations and reaction mixtures comprising at least one polymerase, α-cyclodextrin, and polysorbate 20. Also disclosed are related methods for preparing the disclosed formulations and reaction mixtures, as well as related kits and methods for nucleic acid amplification. The formulations and reaction mixtures are useful, for example, in mitigating the inhibitory effect of sample extraction detergents on nucleic acid amplification reactions.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
77.
COMPOSITIONS AND METHODS FOR DETECTION OF VIRAL PATHOGENS IN SAMPLES
This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
78.
COMPOSITIONS AND METHODS FOR DETECTION OF VIRAL PATHOGENS IN SAMPLES
This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
79.
COMPOSITIONS AND METHODS FOR DETECTION OF VIRAL PATHOGENS IN SAMPLES
This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
80.
COMPOSITIONS AND METHODS FOR DETECTION OF VIRAL PATHOGENS IN SAMPLES
This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
A microfluidic cartridge for detecting one nucleic acid of a sample, including a plurality of functional volumes split into functional areas and a fluidic network of microchannels. At least three functional areas are fluidly connected to a central distribution hub of fluids by one or more hub-connected microchannels, the central distribution hub being capable of pumping and injecting fluids from a first functional area to a second functional area by passing through the central distribution hub; and at least three valves of hub-connected microchannels are arranged so that the at least three valves are adapted to be actuated mechanically by a single external cam-driven actuator.
A method of loading consumables into a sample processing instrument. In the method, a tray storing sample processing consumables is aligned with a support cavity defined by a support of the sample processing instrument. A flange is contacted with a portion of the support, where the flange extends outward from a wall of a cover coupled to the tray. The cover is decoupled from the tray by pressing a panel of the cover, and the tray is seated in the support cavity.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Nucleic acid oligomers, including amplification oligomers and detection probes, for detection of human adenovirus nucleic acid by binding to the penton gene. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits and related methods for preparing aqueous reaction mixtures from dried formulations. Nucleic acid oligomers and methods for nucleic acid amplification and detection of one or more adenovirus subgroups A, B, C, D, E and F are also disclosed.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
A jig assembly for connecting first and second consoles with predefined relative positioning includes first and second positioning pins attached or attachable to the first console and a positioning connection bracket attached or attachable to the second console and including a longitudinal span and first and second transverse legs including first and second pin recesses at respective free ends thereof. The first and second pin recesses receive the first and second positioning pins, respectively, when the first and second consoles are moved laterally toward each other. The first and second transverse legs may be attached to the first console with a first and second leg attachment brackets, respectively. The first and second positing pins and the first and second leg attachment brackets may be fixed with respect to the first console by attachment to a mounting rail that is fixed to the first console.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
There is disclosed a composition of an aqueous solution comprising, consisting or consisting essentially of a flap endonuclease, a bulking agent and an organic buffer, wherein the aqueous solution has an inorganic salt concentration of 5 mM or less and wherein the composition is substantially free of glycerol.
Yersinia enterocoliticaVibrioE. coliPlesiomonas shigelloidesPlesiomonas shigelloides target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
87.
RECEPTACLE TRANSPORT SYSTEM FOR AN ANALYTICAL SYSTEM
A receptacle delivery system for an instrument includes a carriage, a puck rotatably supported by the carriage, a first electric motor, and a second electric motor. The puck comprises a plurality of spring-loaded fingers arranged around a vertical axis and configured to removably support a receptacle therebetween. The first electric motor is configured to move the carriage between a first location and a second location of the instrument. The second electric motor is configured to rotate the puck about the vertical axis.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
88.
RECEPTACLE TRANSPORT SYSTEM FOR AN ANALYTICAL SYSTEM
A receptacle delivery system for an instrument includes a carriage, a puck supported by the carriage, and a first shelf. The carriage is configured to move from a first location of the instrument to a second location of the instrument. The puck is configured to removably support a receptacle such that a longitudinal axis of the receptacle is substantially coincident with a vertical axis of the puck. The first shelf is positioned at the second location of the instrument and comprises (a) a base extending substantially transverse to the vertical axis of the puck and (b) a first opening defined by the base. When the carriage is positioned at the second location, the longitudinal axis of a receptacle seated in the puck extends through the first opening.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
89.
Receptacle transport system for an analytical system
A method of delivering a receptacle to an instrument includes the steps of supporting a receptacle in a carriage, activating an electric motor to move the carriage between a first location and a second location of the instrument while the receptacle is supported by the carriage, applying a clamping force to the receptacle as the carriage moves from the first location to the second location, and releasing the clamping force from the receptacle as the carriage moves from the second location to the first location.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
90.
COMPOSITIONS AND KITS FOR DETECTING THE PRESENCE OF A HYPERVIRULENT CLOSTRIDIUM DIFFICILE STRAIN
The present invention provides a nucleic acid amplification based method for detecting a hypervirulent Clostridium difficile strain in a biological sample. The present invention is based on the use of oligonucleotide primers and probes specific to negative and positive markers in hypervirulent Clostridium difficile genome.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Automated in vitro specimen analyzer, handling and/or
processing instruments for laboratory use. Automated in vitro specimen analyzer, handling and/or
processing instruments for medical use.
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Automated in vitro specimen analyzer, handling and/or
processing instruments for laboratory use. Automated in vitro specimen analyzer, handling and/or
processing instruments for medical use.
93.
Scissors actuator configured to translate a support platform in either of opposed lateral directions
A scissors actuator is configured to translate a support platform in either of opposed lateral directions with respect to a base frame and includes a first arm, a second arm, wherein the first and second arms are rotatably connected at intermediate positions between their respective first and second ends, the first arm is pivotably attached at its first end to the base frame, and the second arm and is pivotably attached at its second end to the support platform. A first slide is disposed within a first linear track formed in the support platform and includes a bearing protruding from a side of the first slide for rolling contact with a side of the first linear track formed in the support platform. The first arm is pivotably and translatably attached at its second end to the support platform by the first slide. A second slide is disposed within a second linear track formed in the base frame and includes a bearing protruding from a side of the second slide for rolling contact with a side of the second linear track formed in the base frame. The second arm is pivotably and translatably attached at its first end to the base frame by the second slide.
A receptacle delivery system for an instrument includes a carriage, a puck supported by the carriage, and a first shelf. The carriage is configured to move from a first location of the instrument to a second location of the instrument. The puck is configured to removably support a receptacle such that a longitudinal axis of the receptacle is substantially coincident with a vertical axis of the puck. The first shelf comprises (a) a base extending substantially transverse to the vertical axis of the puck and (b) a first opening defined by the base, and when the carriage is positioned at the second location, the longitudinal axis of a receptacle seated in the puck extends through the first opening.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
95.
Receptacle transport system for an analytical system
A method of delivering a receptacle to an instrument includes the steps of supporting a receptacle containing a fluid on a carrier, transporting the carrier supporting the receptacle on a conveyor extending adjacent to each of a plurality of instruments, transferring the receptacle from the carrier to a puck supported on a carriage when the carriage is positioned at a first location, moving the carriage with the receptacle seated in the puck from the first location to a second location within an instrument of the plurality of instruments, and drawing at least a portion of the fluid from the receptacle seated in the puck into a tip associated with a fluid extraction device of the instrument when the carriage is positioned at the second location.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/06 - Supports de tubes à essaiPorte-tubes à essai
B65G 43/08 - Dispositifs de commande actionnés par l'alimentation, le déplacement ou le déchargement des objets ou matériaux
B65G 47/90 - Dispositifs pour saisir et déposer les articles ou les matériaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
96.
Methods and compositions for the selection and optimization of oligonucleotide tag sequences
Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.
An apparatus for incubating the contents of a plurality of receptacles and for detecting a signal emitted by the contents of each of the receptacle includes a plurality of receptacle holders configured to incubate the contents of each a plurality of receptacles held by each of the receptacle holders. A controller is coupled to each of the receptacle holders and is configured to independently control an incubation temperature of each of the receptacle holders to independently control a temperature at which the receptacles held by each of the receptacle holders is incubated. At least one signal detector is configured to detect a signal emitted by the contents of each of the receptacles held in each of the receptacle holders, and a signal detector indexer is configured to successively optically couple each signal detector with each of the receptacles held in each of the receptacle holders to detect optical emissions from each successively coupled receptacle.
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G02B 6/06 - Guides de lumièreDétails de structure de dispositions comprenant des guides de lumière et d'autres éléments optiques, p. ex. des moyens de couplage formés par des faisceaux de fibres la position relative des fibres étant la même aux deux extrémités, p. ex. pour transporter des images
G02B 6/08 - Guides de lumièreDétails de structure de dispositions comprenant des guides de lumière et d'autres éléments optiques, p. ex. des moyens de couplage formés par des faisceaux de fibres la position relative des fibres étant la même aux deux extrémités, p. ex. pour transporter des images le faisceau de fibres ayant la forme d'une plaque
G02B 6/42 - Couplage de guides de lumière avec des éléments opto-électroniques
98.
COMPOSITIONS AND METHODS FOR BIOLOGICAL SAMPLE PROCESSING
Methods, apparatuses, and systems are provided for processing a biological sample. Exemplary methods comprise transferring a swab associated with an aliquot of the biological sample into a sample transport media. The methods can provide efficient transfer of a target material such as cells or nucleic acid into the sample transport media for extraction, amplification, and detection of the target material.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12N 9/54 - Protéinases provenant de bactéries les bactéries étant du genre Bacillus
C12Q 1/37 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase faisant intervenir une peptidase ou une protéinase
99.
APPARATUS FOR APPLYING THERMAL ENERGY TO A RECEPTACLE AND DETECTING AN EMISSION SIGNAL FROM THE RECEPTACLE
An apparatus for incubating a plurality of receptacles includes one or more thermally-conductive receptacle holders, each comprising a plurality of receptacle wells. A thermally-conductive support is associated with each of the receptacle holders, and a thermal element is positioned between the associated support and the receptacle holder. A heat sink is in thermal communication with the support and comprises a plurality of spaced-apart fins. An optical fiber associated with each receptacle well has a first end positioned adjacent to or within a through-hole formed in the receptacle well and extends through through-holes formed in the support and the heat sink aligned with the through-hole of the receptacle well. Each of the optical fibers is positioned between two adjacent fins of the plurality of fins.
An apparatus comprises a thermally conductive receptacle holder including a plurality of receptacle wells configured to receive receptacles, a plurality of optical fibers having first and second ends, wherein each first end is in optical communication with one of the receptacle wells and each second end is in optical communication with an excitation signal source and/or an emission signal detector, one or more thermal elements positioned proximal to the receptacle holder for altering the temperature of the receptacle holder, a heat sink in thermal communication with the receptacle holder, and one or more thermal devices disposed within the heat sink for pre-heating the heat sink.
