Thermothelomyces heterothallica (formerly Myceliophthora thermophila) genetically modified to produce glycoproteins with N-glycans of mammalian proteins (particularly human, companion animal and other animal proteins) are provided, comprising deletion or disruption of the alg3 gene, expression of ER-targeted Mannosidase 1 (alpha-1.2-Mannosidase), and expression of ER-targeted Glucosidase 2 alpha-subunit. The Th. heterothallica may also further comprise heterologous GlcNAc transferase 1 (GNT1), GlcNAc transferase 2 (GNT2), STT3 subunit of a heterologous oligosaccharyltransferase and galactosyltransferase.
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, capable of producing multiple immune-active molecules, in particular viral antigens, with antigens from various coronavirus variants being a specific example.
The present disclosure concerns using transgenic fungus to express recombinant viral antigens. The composition, production, and administration of vaccines comprising those viral antigens also are disclosed. In some embodiments, these viral antigens can be used to formulate a vaccine against Newcastle Disease. These vaccines can be administered, for example, via intramuscular injection.
VTT TECHNICAL RESEARCH CENTRE OF FINLAND LTD. (Finlande)
DYADIC INTERNATIONAL (USA), INC. (USA)
Inventeur(s)
Zrachya, Avi
Finger, Avner
Valbuena, Noelia
Tchelet, Ronen
Markku, Saloheimo
Abrégé
The present disclosure concerns using transgenic fungus to express recombinant viral antigens. The composition, production, and administration of vaccines comprising those viral antigens also are disclosed. In some embodiments, these viral antigens can be used to formulate a vaccine against Newcastle Disease. These vaccines can be administered, for example, via intramuscular injection.
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, having reduced activity or expression of KEX2 and/or ALP7, said filamentous fungi is capable of producing elevated amounts and stability of an exogenous protein.
Thermothelomyces heterothallicaMyceliophthora thermophilaalg3Th. heterothallicaTh. heterothallica may also further comprise heterologous GlcNAc transferase 1 (GNT1), GlcNAc transferase 2 (GNT2), STT3 subunit of a heterologous oligosaccharyltransferase and galactosyltransferase.
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, capable of producing multiple immune-active molecules, in particular viral antigens, with antigens from various coronavirus variants being a specific example.
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons
C07K 16/08 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus
C07K 14/165 - Coronaviridae, p. ex. virus de la bronchite infectieuse aviaire
9.
GENETICALLY-MODIFIED FILAMENTOUS FUNGI FOR PRODUCTION OF EXOGENOUS PROTEINS HAVING REDUCED OR NO N-LINKED GLYCOSYLATION
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica capable of producing cannabinoids and precursors thereof, particularly of producing cannabigerolic acid (CBGA) and/or cannabigerovarinic acid (CBGVA) and products thereof, including tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and cannabidivarinic acid (CBDVA), and use thereof for producing said precursors and cannabinoids.
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, having reduced activity or expression of KEX2 and/or ALP7, said filamentous fungi is capable of producing elevated amounts and stability of an exogenous protein.
Thermothelomyces heterothallicaThermothelomyces heterothallica, having reduced activity or expression of KEX2 and/or ALP7, said filamentous fungi is capable of producing elevated amounts and stability of an exogenous protein.
Thermothelomyces heterothallica Myceliophthora thermophilaalg3alg11Th.heterothallicaheterothallica may also further comprise heterologous GlcNAc transferase 1 (GNT1), GlcNAc transferase 2 (GNT2), STT3 subunit of a heterologous oligosaccharyltransferase and galactosyltransferase.
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica capable of producing cannabinoids and precursors thereof, particularly of producing cannabigerolic acid (CBGA) and/or cannabigerovarinic acid (CBGVA) and products thereof, including tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and cannabidivarinic acid (CBDVA), and use thereof for producing said precursors and cannabinoids.
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes to improve the digestability of animal feed and in a variety of other processes, including food and beverage processing, baking, washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to enzymes of fungal origin classified as oxidoreductases and produced by fungi. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including disinfection food, deinking and biobleaching of flour, cotton, paper and pulp, and the treatment of pollution. The invention also relates to a method to aid in the conversion of lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to the degradation of protein and novel combinations of enzymes, including those that provide a synergistic release of amino acids or lower molecular weight proteins. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing or treating of clothing or fabrics, detergent processes, cleaning of carpets, leather treatment, animal feed, food processing, baking, increasing the protein yield of food, modification of food taste, processing of proteins in protein production, processing of beverages, medical treatment, and cosmetic treatment, and bio fuels.
This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes to improve digestability of animal feed and in a variety of other processes, including food and beverage processing, baking, washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib, CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.
brevets
