Abrégé

The invention provides a latex agglutination method-mediated target substance measurement method, and a reagent therefor, allowing for an accurate measurement in both a low concentration region and a high concentration region. The latex agglutination method-mediated target substance measurement method comprises reacting a sensitized latex particle suspension with a target substance, and then measuring, from the amounts of optical changes, the agglutination of the sensitized latex particles. In the method, the volume-based mean particle diameter of the sensitized latex particles prior to sensitization is 80 nm to 335 nm, the final concentration of the sensitized latex particles in the reaction system is 0.005 to 0.10 w/v%, the final concentration, in the reaction system, of particles having a particle diameter of 80 nm or smaller for the sensitized latex particles prior to sensitization, is 0.09 w/v% or lower, and the amounts of optical changes comprise the amount of change in absorbance and the amount of change in scattered ray.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

The present invention aims to provide: an immunochromatographic test kit for controlling the development of analytes on an immunochromatographic test piece and for appropriately controlling processing using acidic reagents, nitrite, and neutralizing reagents; and an immunochromatography method using said kit.　The immunochromatographic test kit includes an immunochromatographic test piece that includes: a sample pad to which is added an analyte that has mixed therein (i) a nitrite solution or an acidic solution and (ii) nitrite or an acidic solution; a marker region that includes a marker antibody marking an antibody for sugar chain antigens; and a detection region in which the antibody for sugar chain antigens is immobilized. The immunochromatographic test piece forms an antibody–sugar chain antigen–marker antibody complex in the detection region and measures the sugar chain antigens. The immunochromatographic test piece is for extracting and measuring sugar chain antigens in the analyte and has: a region impregnated with a neutralizing reagent, upstream from the marker region; and, upstream from the region impregnated with the neutralizing reagent, a region impregnated with a solid acidic reagent, when an analyte mixed with nitrite is used, or a region impregnated with nitrite, when an analyte mixed with acidic solution is used. The nitrite or acidic solution include at least one selected from the group consisting of polyoxyethylene octyl phenyl ether, a quaternary ammonium compound, phosphoric acid, sodium hydroxide, N-acetyl-L-cysteine, NaCl, PVA, BSA, and NaBr.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

The purpose of the present invention is to provide an immunochromatographic test piece for controlling the development of a specimen on the immunochromatographic test piece so as to appropriately control a treatment with an acidic reagent, a nitrite and a neutralizing reagent, and an immunochromatographic method in which the test piece is used.　An immunochromatographic test piece for extracting and measuring a carbohydrate antigen in a specimen, said immunochromatographic test piece comprising: a sample pad to which the specimen mixed with a nitrite or an acidic solution is added; a label region containing a labeled antibody which is prepared by labeling an antibody to the carbohydrate antigen; and a detection region in which the antibody for the carbohydrate antigen is immobilized, wherein an antibody–carbohydrate antigen–labeled antibody complex is formed in the detection region to thereby measure the carbohydrate antigen. The immunochromatographic test piece, which has a region impregnated with a neutralizing reagent upstream of the label region and, further upstream of the region impregnated with the neutralizing reagent, a region impregnated with a solid acidic reagent in the case of using the specimen as a mixture with the nitrite, or a region impregnated with a nitrite in the case of using the specimen as a mixture with the acidic solution, is characterized in that a hydrophobic material is used in the region impregnated with the nitrite or the solid acidic reagent.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

A purpose of the present invention is to provide an immunochromatography test piece for controlling the spread of an analyte on said immunochromatography test piece and appropriately controlling a process that uses an acidic reagent, a nitrite, and a neutralizing reagent, as well as to provide an immunochromatography method that uses said test piece.　This immunochromatography test piece for extracting and measuring a carbohydrate antigen in an analyte includes a sample pad that adds the analyte, said analyte having been mixed with a nitrite or an acidic solution, a marker region that includes a marker antibody resulting from marking an antibody of the carbohydrate antigen, and a detection region where an antibody of the carbohydrate antigen has been immobilized, and the immunochromatography test piece measures the carbohydrate antigen by causing an antibody-carbohydrate antigen-marker antibody complex to form in the detection region. The immunochromatography test piece also has a region that is upstream of the marker region and has been impregnated with a neutralizing reagent, and further upstream of this region impregnated with the neutralizing reagent, the immunochromatography test piece has a region that has been impregnated with a solid acidic reagent in the case when an analyte that has been mixed with a nitrite is used, or has a region that has been impregnated with a nitrite in the case when an analyte that has been mixed with an acidic solution is used. This immunochromatography test piece is characterized in that a hydrophilic material is used in the region that has been impregnated with the nitrite or the solid acidic reagent.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

The present invention makes it possible to improve the usefulness of device-based testing and assessment. The testing-assessment device according to the present invention is a testing-assessment device for immunochromatography in which a liquid specimen that potentially contains an analyte is expanded in an analyte detection area via a labeling-substance-containing area of a test strip, and an assessment of negative or positive is made on the basis of the coloration exhibited by the analyte detection area. The device comprises a measuring part for obtaining, at least once, data on a coloration index constituting an index associated with coloration state for at least part of the analyte detection area, and a processing part that performs the assessment on the basis of the coloration index data. The processing part delivers a negative assessment if the coloration state of the analyte detection area is in a negative state in at least one image out of a maximum of N (wherein N is greater than 1) obtained images, and delivers a positive assessment if the coloration state of the analyte detection area is in a positive state in all N images.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is a technology whereby determination in an appropriate detection region is made possible in automatic determination of an immunochromatographic assay. An immunochromatographic assay determination device in which a liquid sample that potentially includes a substance to be detected spreads into a detection region for the substance to be detected via a labeled-substance-containing region of a test strip, and a determination of negative or positive is made from the coloration state of the detection region for the substance to be detected, wherein an observation unit acquires coloration index data as an index relating to the coloration state for a range including the position of at least a portion of a sample spreading detection region for indicating, by the coloration state thereof, that a liquid sample has reached the sample spreading detection region, the sample spreading detection region being downstream in the spreading direction from the detection region for the substance to be detected in the test strip, and a processing unit specifies the sample spreading detection region from the coloration index data, specifies the detection region for the substance to be detected in a position a predetermined inter-region distance upstream from the sample spreading detection region in the data of the coloration index data, and makes a determination of negative or positive on the basis of the coloration state of the detection region for the substance to be detected.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is an inactivated whole-virus influenza vaccine of which the antibody induction ability is maintained or enhanced and which causes reduced side reactions. A method for preparing an inactivated whole-virus influenza vaccine using an embryonated chick assay, comprising the step of subjecting a virus solution containing influenza virus whole particles collected from an embryonated chicken egg to a hypotonic treatment.

Classes IPC  ?

• A61K 39/145 - Orthomyxoviridae, p. ex. virus de l'influenza
• A61K 47/46 - Ingrédients de constitution indéterminée ou leurs produits de réaction, p. ex. peau, os, lait, fibre de coton, coquille d’œuf, fiel de bœuf ou extraits de plante
• A61P 31/16 - Antiviraux pour le traitement des virus ARN de la grippe ou des rhinovirus

Abrégé

Provided are: a mucosal adjuvant which is useful for the preparation of a mucosal vaccine having high mucosal immunogenicity and high safety; and a mucosal vaccine composition containing the mucosal adjuvant. A mucosal adjuvant comprising a TGDK.

Classes IPC  ?

• A61K 39/39 - Préparations médicinales contenant des antigènes ou des anticorps caractérisées par les additifs immunostimulants, p. ex. par les adjuvants chimiques
• A61K 38/06 - Tripeptides
• A61K 39/145 - Orthomyxoviridae, p. ex. virus de l'influenza
• A61P 31/16 - Antiviraux pour le traitement des virus ARN de la grippe ou des rhinovirus
• A61P 37/04 - Immunostimulants
• A61P 43/00 - Médicaments pour des utilisations spécifiques, non prévus dans les groupes
• C07K 5/033 - Peptides ayant jusqu'à quatre amino-acides dans une séquence entièrement déterminéeLeurs dérivés contenant au moins une liaison peptidique anormale dans laquelle au moins un epsilon- ou un zêta-amino-acide est impliqué

Abrégé

Disclosed is a method for easily aiding the detection of primary biliary cholangitis by means of a blood test. This method for aiding the detection of primary biliary cholangitis involves measuring, in a test blood sample isolated from an organism, at least one amount selected from the group consisting of LDL-TG, RLP-C, LDL-TG/LDL-C, sd LDL-C, LDL-C/sd LDL-C, LDL-TG/sd LDL-C, total TG, and total TG/total CHO. When the LDL-TG level is used as an indicator, a higher LDL-TG level than that of a healthy individual indicates a high likelihood of primary biliary cholangitis.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Provided is immunochromatography in which surface plasmon resonance is amplified without complicated production steps or inspection procedures being required. In this immunochromatography, a complex is formed in which resonant particles for producing surface plasmon resonance have been caused to accumulate on separate holding particles via a substance under test, and the substance under test is detected through the capturing of the complex on an immunochromatography test piece and the detection of the complex. This immunochromatography is more sensitive than immunochromatography only using particles for producing surface plasmon resonance.

Classes IPC  ?

• G01N 21/41 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

Provided are: a method which makes it possible to aid the detection of nonalcoholic steatohepatitis (NASH) by means of a simple operation which is not dependent on the abilities of a technician and which is minimally invasive compared to liver biopsies; and a method which makes it possible to aid the determination of the degree of advancement of pathological conditions relating to NASH.　The present invention provides a method for aiding the detection of nonalcoholic steatohepatitis, the method involving the measurement of the amount of LDL-TG and/or ApoE rich HDL-C present in a test blood sample isolated from an organism. The present invention also provides a method for aiding the determination of the degree of advancement of at least one pathological condition relating to nonalcoholic steatohepatitis and selected from the group consisting of fatty degeneration, inflammation, ballooning degeneration, and fibrosis, the method involving the measurement of the amount of LDL-TG and/or ApoE rich HDL-C present in a test blood sample isolated from an organism.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Provided is a method that, in a method for quantifying lipoprotein cholesterol in a test sample, even when the test sample has been diluted, minimizes reductions in measurement values for lipoprotein cholesterol and improves calibration curve linearity at low measurement values for cholesterol. Also provided is a quantification kit that is used for the method. The present invention provides: a method that, in quantification of lipoprotein cholesterol in a diluted test sample, minimizes reductions in measurement values for the lipoprotein cholesterol that is a measurement target, by executing, in the presence of peroxidase, a step of transferring out of a reaction system lipoprotein cholesterol that is not the measurement target and is present in the diluted test sample; and a method for quantifying lipoprotein cholesterol. The present invention also provides a quantification kit for lipoprotein cholesterol that is used for the method according to the present invention.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Provided are: a method for quantifying a lipoprotein cholesterol in a sample of interest more accurately; and a quantification reagent and a quantification kit which can be used in the method. The present invention is a lipoprotein cholesterol quantification method for quantifying a lipoprotein cholesterol in a lipoprotein-containing sample of interest optionally using a quantification reagent, the method including adding a phospholipid to the sample or the quantification reagent. The present invention is also a reagent for use in the quantification of cholesterol in a lipoprotein, which can be used in the method of the present invention and contains a phospholipid. The present invention is also a kit for quantifying a lipoprotein cholesterol, which can be used for the method of the present invention and includes a phospholipid.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase

Abrégé

Disclosed are: a method for assisting the determination of the risk of a cardiovascular disease, a coronary heart disease or stroke; and a method for assisting the diagnosis of stroke. The method for assisting the determination of the risk of a cardiovascular disease, a coronary heart disease or stroke comprises measuring the LDL-TG level in blood isolated from a living body, wherein, when the measured LDL-TG level is high, it is determined that the risk of a cardiovascular disease, a coronary heart disease or stroke is high. The method for assisting the diagnosis of stroke comprises measuring the LDL-TG level in blood isolated from a living body, wherein, when the measured LDL-TG level is high, it is determined that the possibility of the development of stroke is high.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Disclosed are: a method for quantifying cholesterol in a HDL, whereby it becomes possible to selectively, simply and correctly quantify HDL cholesterol in a sample of interest containing an HDL as well as another lipoprotein such as a LDL without requiring any complicated fractionation/separation procedure; and a method for selectively eliminating cholesterol, which can be employed in the aforementioned method. A method for eliminating cholesterol in a lipoprotein other than a high-density lipoprotein in a sample of interest out of a reaction system includes allowing (i) phospholipase and/or sphingomyelinase and (ii) a nonionic surfactant to act on the sample.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
• C12Q 1/42 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une phosphatase
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Provided is a method for detecting, with high sensitivity and high specificity, neurodegenerative diseases selected from the group consisting of human Alzheimer's disease (AD), frontotemporal lobar degeneration (FTLD), and amyotrophic lateral schlerosis (ALS). A method for detecting neurodegenerative diseases selected from the group consisting of human Alzeimer's disease (AD), frontotemporal lobar degeneration (FTLD), and amyotrophic lateral schlerosis (ALS), the method including the following steps: (i) a step for measuring MARCKS protein phosphorylated on serine 46 in a test specimen collected from a subject, and also measuring non-phosphorylated MARCKS protein; (ii) a step for calculating the DO value represented by Formula 1 from measurement values obtained in step (i) (in the formula, "pSer46-MARCKS" indicates the quantity of MARCKS protein phosphorylated on serine 46, and "non-phosphorylated-MARCKS" indicates the quantity of non-phosphorylated MARCKS protein); and (iii) a step for detecting a neurodegenerative disease using the DO value as an index.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 27/62 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'ionisation des gaz, p. ex. des aérosolsRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant les décharges électriques, p. ex. l'émission cathodique

Abrégé

The purpose of the present invention is to provide a testing kit that makes it possible to reliably determine whether a substance to be detected is present. This testing kit 11 is provided with a test strip 21 in which a sample dripping pad 31, a labeling substance holding pad 41, and a fixing membrane 51 are connected, in that order, and as a dripped liquid sample progressively spreads toward the fixing membrane 51, the substance to be detected is detected at the fixing membrane 51. The test strip 21 has a region where the labeling substance holding pad 41 and fixing membrane 51 overlap at least partially and is provided with a pressing part 910 for pressing a portion in this region in a direction orthogonal to the direction in which the liquid sample spreads. In a flow area P where the liquid sample spreads in the fixing membrane 51, which is outside the flow area R where the liquid sample spreads directly below the region pressed by the pressing part 910, it is possible to confirm, from outside, whether the substance to be detected has been detected.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is a biomarker for detecting colorectal cancer at an earlier stage. A colorectal cancer biomarker for detecting colorectal cancer, which includes at least one protein selected from 22 proteins, i.e., proteins 1 to 22, or at least one peptide selected from partial peptides of proteins 1 to 22: 1. annexin A11; 2. annexin A3; 3. annexin A4; 4. tenascin-N; 5. transferrin receptor protein 1; 6. glucose transporter 1; 7. complement component C9; 8. CD88 antigen; 9. 78-kDa glucose-regulated protein; 10. α-1-acid glycoprotein; 11. matrix metalloprotease 9; 12. angiopoietin-1; 13. CD67 antigen; 14. mucin-5B; 15. adapter protein GRB2; 16. annexin A5; 17. olfactomedin-4; 18. neutral amino acid transporter B(0); 19. tripeptidyl peptidase 1; 20. heat shock-related 70-kDa protein 2; 21. proteasome subunit α type-5; and 22. neutrophil gelatinase-associated lipocalin.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
• C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
• C12N 9/64 - Protéinases provenant de tissu animal, p. ex. rennine
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer

Abrégé

Provided is an immunochromatography test piece that is for immunochromatography in which a carbohydrate antigen is extracted on the immunochromatography test piece by nitrous acid extraction and then measured. The immunochromatography test piece prevents non-specific reactions by efficiently and continuously bringing a development liquid that contains nitrous acid into contact with a neutralizing reagent such that the development liquid is neutralized. An immunochromatography test piece that includes a sample pad to which is added a sample that has been mixed with a nitrite or with an acidic solution, a label area that includes a labeled antibody that is formed by labeling an antibody for a carbohydrate antigen, and a detection area that has an antibody for the carbohydrate antigen solidified thereon. The immunochromatography test piece makes antibody-carbohydrate antigen-labeled antibody composites form in the detection area and measures the carbohydrate antigen. The immunochromatography test piece has an area that is upstream of the label area and is impregnated with a neutralizing reagent. The immunochromatography test piece also has an area that is upstream of the area that is impregnated with the neutralizing reagent. When a sample that has been mixed with a nitrite is to be used, the area upstream of the area impregnated with the neutralizing agent is impregnated with a solid, acidic reagent, and when a sample that has been mixed with an acidic solution is to be used, the area upstream of the area impregnated with the neutralizing agent is impregnated with a nitrite. The immunochromatography test piece is for extracting the carbohydrate antigen from the sample and measuring the carbohydrate antigen. The area that is impregnated with the neutralizing agent comprises filter paper or glass filter paper that is highly absorbent, has high water-retention, and has low or continuous discharge. The high water-absorption and high water-retention of the area that is impregnated with the neutralizing agent make it possible to fully neutralize an acidic solution that includes the carbohydrate antigen, and the low or continuous discharge of the area that is impregnated with the neutralizing agent makes it possible to keep any remaining acidic solution from reaching the detection area or to maintain continuous development of a fully neutralized test liquid in the detection area and thereby suppress non-specific reactions.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

The purpose of the present invention is to provide a method or an immunochromatographic test piece, whereby the speed or direction of development of a specimen upon the immunochromatographic test piece is controlled and treatment by an acidic reagent, a nitrite, and a neutralizing reagent is appropriately controlled. An immunochromatographic test piece including a sample pad to which a specimen is added that has nitrite or an acidic solution mixed therein, a label region including a labeled antibody being an antibody for carbohydrate antigens that has been labeled, and a detection region having the antibody for carbohydrate antigens immobilized therein. The immunochromatographic test piece forms an antibody–carbohydrate antigen–labeled antibody complex in the detection region and measures carbohydrate antigens. The immunochromatographic test piece is for extracting and measuring carbohydrate antigens in a specimen and has: a region having the neutralizing reagent impregnated therein, upstream from the label region; a region having a solid acidic reagent impregnated therein when using a specimen having nitrite mixed therein, further upstream from the region having the neutralizing reagent impregnated therein; and a region having nitrite impregnated therein, when using a specimen having an acidic solution mixed therein. The immunochromatographic test piece has a resin sheet interposed between the region having the solid acidic reagent or nitrite impregnated therein and the region having the neutralizing reagent impregnated therein, same being interposed so as to suppress the movement of reagent or movement of specimen solution between the two regions.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

Provided are a method and an immunochromatographic device, whereby sufficiently sensitive measurement can be performed by conducting nitrite extraction over a sufficient period, in an immunochromatographic method that extracts and measures carbohydrate antigens by using nitrite extraction upon an immunochromatography test piece. This immunochromatographic device has a specimen addition port in a test piece sample pad and comprises an immunochromatography test piece and a container housing the test piece. The immunochromatography test piece is for extracting and measuring carbohydrate antigens in the specimen and: includes the sample pad to which a specimen having a nitrite or an acidic solution mixed therein is added, a label region including a labeled antibody being a labeled antibody for carbohydrate antigens, and a detection region having the antibody for carbohydrate antigens immobilized therein; forms an antibody–carbohydrate antigen–labeled antibody complex in the detection region; measures the carbohydrate antigens; has a region impregnated with a neutralizing reagent, upstream of the label region; has a region impregnated with a solid acidic reagent when using a specimen mixed with nitrite, further upstream of said region impregnated with the neutralizing reagent; and has a region impregnated with nitrite when using a specimen mixed with an acidic solution. The immunochromatographic device: (i) has a wide specimen addition port for promoting extraction of carbohydrate antigens by the nitrite and the solid acidic reagent, by holding an added specimen sample solution and supplying the specimen sample solution for a short period to the region impregnated with the solid acidic reagent or the nitrite; and (ii) does not have a gap between the addition port and the sample pad, so as to ensure that the sample does leak or get spilled from the addition port.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

Provided are: a method for agglutinating erythrocytes and a method for separating erythrocytes, wherein erythrocytes can be instantaneously agglutinated into a sufficient size from a blood sample, and the erythrocytes can be completely separated from the blood sample; and a hemagglutination reagent. The method for agglutinating erythrocytes comprises adding a solution, containing a cholic acid-based surfactant and an acid, to a blood sample. The method for separating erythrocytes comprises separating the erythrocytes agglutinated by the method for agglutinating erythrocytes. The hemagglutination reagent contains a cholic acid-based surfactant and an acid.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

The purpose of the present invention is to provide an immunochromatography test piece that enables the specific measurement of a sugar chain antigen. The present invention is an immunochromatography test piece for measuring a sugar chain antigen, and includes a sample pad to which a specimen is added, a marker area including a marker antibody obtained by labeling an antibody with a sugar chain antigen, a detection area in which an antibody to the sugar chain antigen is immobilized, and in which an antibody-sugar chain antigen-marker antibody complex is formed in the detection area. The method for measuring a sugar chain antigen in a sample by immunochromatography uses an immunochromatography test piece having an area impregnated with a neutralizing agent upstream of the marker area, and has an area impregnated with tartaric acid further upstream than said area impregnated with neutralizing reagent. The method for measuring a sugar chain antigen in a specimen by immunochromatography includes mixing the specimen with a nitrite solution and adding to a sample pad of the immunochromatography test piece. In the area impregnated with tartaric acid, a sugar chain antigen is extracted from the specimen by the action of nitrous acid generated by the reaction between nitrite and tartaric acid, an acidic solution including the sugar chain antigen is neutralized in the area including a neutralizing reagent, and an antigen-sugar chain antigen-marker antibody is formed in the detection area.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• C07K 14/005 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de virus
• C07K 14/195 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries
• C07K 14/29 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Richettsiales (O)
• C07K 14/295 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Chlamydiales (O)
• C07K 14/30 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Mycoplasmatales, p. ex. organismes analogues aux Pleuropneumonia [PPLO]
• C07K 14/44 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de protozoaires
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

The purpose of the present invention is to provide an immunochromatography test piece and specimen adding device that make it possible to specifically measure a sugar chain antigen, and an immunochromatography method using same. This immunochromatography test method for measuring sugar chain antigen entails mixing a specimen and a nitrite solution, and subsequently bringing the mixture into contact with tartaric acid, and performing a step, in which the sugar chain antigen in the specimen is extracted, in a filtration step.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 1/10 - Dispositifs pour prélever des échantillons à l'état liquide ou fluide
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

Disclosed are a reagent and a method which are for more specifically quantifying cholesterol (TRL-C) in triglyceride-rich lipoprotein in a test sample, and do not require complex manipulation. The method for quantifying cholesterol in triglyceride-rich lipoprotein comprises: a step (1) for selectively removing cholesterol in lipoproteins other than triglyceride-rich lipoprotein (TRL) by activating a surfactant and a cholesterol esterase having a molecular weight of more than 50 kDa; and a step (2) for quantifying the remaining cholesterol (TRL-C) in the TRL.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Disclosed are: an immunochromatographic test device by which the presence of an object to be detected can be detected or the amount thereof can be determined more quickly and sensitively than by conventional measurement methods; and a method for forming a sample addition part of the immunochromatographic test device. The method for forming a sample addition part of the immunochromatographic test device comprises applying a surfactant such as sodium deoxycholate, said surfactant being in the form of a white powder in ordinary state, to the sample addition part and then drying. The immunochromatographic test device comprises the sample addition part that is formed by the aforesaid method.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Disclosed are: an immunoassay method, including an immunological agglutination method, which makes it possible to measure an antigen in an immunoassay accurately and with high sensitivity; and a reagent kit for use in the immunoassay method. The immunoassay method involves performing an antigen-antibody reaction and/or a measurement in the presence of a polyoxyethylene alkyl ether surfactant having a C8-14 alkyl group. The immunoassay reagent kit includes a polyoxyethylene alkyl ether surfactant having a C8-14 alkyl group and an immunoassay reagent.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is a novel method for detecting a test object by immunoassay, the detection method using monoclonal antibodies in the immunoassay yet having improved low sensitivity through use of the monoclonal antibodies. The test object detection method detects a test object in which two or more types of antigens are present for enabling detection of the test object, and is performed by an immunoassay method such as a sandwich method using monoclonal antibodies or antigen-binding fragments thereof for recognizing the two or more types of antigens.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• C07K 16/12 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de bactéries
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• C12N 15/02 - Préparation de cellules hybrides par fusion de plusieurs cellules, p. ex. fusion de protoplastes
• C12P 21/08 - Anticorps monoclonaux

Abrégé

Disclosed is an immunochromatographic test piece and an immunochromatography method using the same, whereby the effect of a substance interfering with immunochromatography method included in a test sample is suppressed, and a substance to be detected in the test sample can be measured accurately and with specificity irrespective of the amount of the test sample subjected to testing. The immunochromatographic test piece according to the present invention is provided with, from an upstream side, a sample pad, a marker region, a detection region, and an absorption band, the immunochromatographic test piece being impregnated upstream from the marker region with a polymer in which a hydrophobic cyclic monomer having an ionic functional group is polymerized.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is a method for easily collecting an antigen contained in a microorganism without requiring the use of a special instrument. A method for collecting an antigen contained in a microorganism, said method comprising passing a sample containing the microorganism through a filter membrane having such pore diameters that the microorganism cannot pass to thereby trap the microorganism in the sample on the filter membrane, then passing a microorganism disruption reagent capable of disrupting the membrane of the microorganism through the filter membrane on which the microorganism has been trapped to thereby disrupt the trapped microorganism on the filter membrane, and then collecting the antigen from a filtrate.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
• C12N 1/06 - Lyse des micro-organismes
• C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

The purpose of the present invention is to reduce background noises in an immunochromatography device to improve the visibility in the immunochromatography device for the purpose of clearly detecting a signal coming from a material to be detected. An immunochromatography device equipped with a membrane that has a detection zone on which an antibody or antigen is immobilized as a capturing substance capable of capturing a substance of interest. In the immunochromatography device, an antigen or antibody labeled with a labeling carrier that is composed of colored particles is used, and a (capturing substance)-(substance of interest)-(labeled antigen or antibody) complex is formed on the capturing substance-immobilized detection zone on the device to detect the substance of interest by the color of the labeling carrier. The device is characterized in that a dye having a color complementary to the color of the labeling carrier is allowed to be contained in a dried state in a constituent member of the device so that the dye can be developed on the device together with a sample when the sample is developed on the device.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

A method for estimating the number of podocytes in urine, said method being characterized by comprising detecting podocalyxin in a urine sediment sample solution. More particularly, the method for estimating the number of podocytes in urine comprises steps (1) to (3). (1) A step for separating urine sediment from urine collected from a subject and solubilizing podocalyxin in the urine sediment to thereby prepare a urine sediment sample solution. (2) A step for detecting podocalyxin in the urine sediment sample solution and calculating the amount of excreted podocalyxin in the urine sediment sample solution. (3) A step for dividing the amount of excreted podocalyxin in the urine sediment sample solution by podocalyxin content per podocyte to thereby calculate the number of podocytes in urine.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

A sample extraction kit is provided with: a flexible cylindrical container (10) which is so configured as to accommodate an extraction liquid therein; two grasping parts (21) which face each other with the cylindrical container (10) sandwiched therebetween; a connection part (25) which connects the two grasping parts (21) that face each other; and folding ribs (23) which are respectively arranged in the grasping parts (21) that face each other and which enables the folding of the cylindrical container (10) and a member to be extracted (EM).

Classes IPC  ?

• G01N 1/10 - Dispositifs pour prélever des échantillons à l'état liquide ou fluide
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

The purpose of this invention is to provide a testing method that uses stomach fluid to allow non-invasive, fast, selective detection of Helicobacter pylori. In this invention, which is a method for detecting Helicobacter pylori infecting the stomach, a sample consisting of stomach contents is collected and Helicobacter pylori present in said stomach contents is detected without the use of a concentration procedure or an incubation procedure.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

[Abstract] Disclosed is a method for measuring a type A influenza virus through an immunoassay employing an anti-type A influenza virus monoclonal antibody having high reactivity to a wide range of subtypes. This method for measuring a type A influenza virus includes a step for measuring a type A influenza virus through an immunoassay utilizing an antigen-antibody reaction of a monoclonal antibody, or an antigen-binding fragment thereof, that specifically reacts with a matrix protein (M1) of a type A influenza virus, and a type A influenza virus in a specimen.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN

Abrégé

Disclosed are: a method for measuring influenza B virus by immunoassay, said method enabling the influenza B virus to be detected specifically and with higher sensitivity than methods of the prior art; and an instrument or kit for the abovementioned method. The method for measuring the influenza B virus involves performing an immunoassay on the influenza B virus by a sandwich method in which two types of monoclonal antibodies or antigen-binding fragments of said monoclonal antibodies are used. The two types of monoclonal antibodies specifically react with the 125th to 248th amino acid regions of the matrix protein (M1) of the influenza B virus, and are simultaneously able to bind with the 125th to 248th amino acid regions of M1.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12N 15/02 - Préparation de cellules hybrides par fusion de plusieurs cellules, p. ex. fusion de protoplastes
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• C12P 21/08 - Anticorps monoclonaux

Abrégé

Provided is a means for enabling immunoassay with sufficient sensitivity, wherein said immunoassay uses the M1 protein of an influenza virus as an antigen to measure the influenza virus in a sample. This sample processing method for an influenza virus immunoassay includes, when performing an immunoassay of an influenza virus using an antibody that binds in an antigen-antibody reaction with the matrix 1 protein of the influenza virus, or using an antigen-binding fragment of said antibody, placing a sample that includes the influenza virus in contact with a sample processing solution that includes a surfactant having at least one group selected from the group consisting of a palmityl group, a stearyl group, and an oleyl group.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

　The object of the present invention is to provide an immunochromatographic test piece that enables both highly sensitive detection of substances to be detected, and a test piece having a simple structure to be achieved, these two attributes normally being incompatible with one another.　This immunochromatographic test piece includes a membrane in which a capture substance that is a ligand that bonds to the subject to be detected is immobilized. Said insoluble carrier particles are accumulated by being captured by a capture substance immobilized on the membrane using insoluble carrier particles to which is bonded a ligand that bonds to the substance to be detected, the membrane is irradiated with light, and the light generated in the region in which said insoluble carrier particles are accumulated and the region in the peripheral area aside from region in which insoluble carrier particles are accumulated is detected, thereby measuring the substance to be detected. Therein, a light-reflecting material is provided on the reverse side of the membrane to the side which is irradiated with light.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 21/64 - FluorescencePhosphorescence
• G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur

Abrégé

Disclosed are: a method and a reagent for quantifying high density lipoprotein 3 (HDL3) in a sample of interest without requiring the employment of complicated operations. A method for quantifying cholesterol in high density lipoprotein 3 comprises reacting a sample of interest with at least one surfactant capable of reacting specifically with high density lipoprotein 3 and quantifying cholesterol. When only one surfactant is used, the surfactant is selected from the group consisting of polyoxyethylene polycyclic phenyl ethers each having a HLB value of 12.5 to 15. When two or more surfactants are used, at least one of the surfactants is selected from the group consisting of polyoxyethylene polycyclic phenyl ethers, and the two or more surfactants are so combined as to have a HLB value of 12.5 to 15 as a whole.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

　The objective of the present invention is to provide non-soluble carrier particles which are for use in a highly rapid and highly sensitive immunoassay, and which enable visual evaluation and highly sensitive device measurement.　These non-soluble carrier particles for visible region coloring, which are labeled using a fluorescent pigment and are used in an immunoassay, absorb little of the fluorescence of the wavelength emitted by the fluorescent pigment.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/545 - Résine synthétique

Abrégé

Provided is an examination kit with which detection can be performed swiftly and with high sensitivity. The present invention is provided with: an instillation area including a section in which a liquid sample is instilled; a labelled-substance holding area which has at least a portion thereof connected downstream in a development direction with respect to the instillation area, and in which a labelled substance is held, said labelled substance being obtained by fixing a label to a substance which specifically binds to a substance to be detected; a development area which is provided with a detection zone for collecting the labelled substance via the substance to be detected, which has at least a portion thereof connected downstream in the development direction with respect to the labelled-substance holding area, and in which the liquid sample is used to develop, in the detection zone, the labelled substance flowing out from the labelled-substance holding area; and a stagnation inhibition means for inhibiting stagnation of the labelled substance in the labelled-substance holding area when the liquid sample is to be developed downstream.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is an examination kit with which detection can be performed swiftly and with high sensitivity. This examination kit is provided with: a first member including an area in which a labelled substance is held, said labelled substance being obtained by fixing a label to a substance which specifically binds to a substance to be detected; and a second member which is provided with a detection zone for collecting the labelled substance via the substance to be detected, which is connected downstream with respect to the first member in a development direction, and in which the labelled substance included in a liquid sample flowing in from the first member is developed in the detection zone. The first member is provided with: an instillation area which is positioned furthest upstream, and which includes a section in which the liquid sample is instilled; a labelled-substance holding area provided with an inclusion section which is connected to the second member, and which includes the labelled substance, and a non-inclusion section which does not include the labelled substance, and which is positioned upstream with respect to the inclusion section; and a back-flow inhibition area which is connected between the instillation area and the non-inclusion section of the labelled-substance holding area, and in which water-absorption capacity is set so as to be higher than that in the instillation region.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

The present invention addresses the problem of providing a method for simply and non-invasively estimating glomerular renal function. Accordingly, the inventors achieved the present invention upon discovering that: there is a strong correlation between estimated glomerular filtration rate (eGFR) and the excretion amount of megalin in urine of patients with renal disease; and GFR can be non-invasively estimated with high probability by measuring megalin in urine.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 33/493 - Analyse physique de matériau biologique de matériau biologique liquide d'urine

Abrégé

The present invention addresses the problem of regulating the action of an immunoassay inhibitory substance contained in a biomembrane-derived specimen and thus accurately and specifically detecting a target substance. An immunoassay method for assaying a target substance in a biomembrane-derived specimen, wherein false negatives can be reduced by treating the specimen with a specimen-treating solution which contains a compound having two or more sulfate groups.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

A method for detecting podocytes in the urine under an optical microscope with the use of an insoluble carrier-bound antibody capable of recognizing a protein that is specifically expressed on podocyte surface. More specifically, the method for detecting podocytes in the urine comprises: (1) a step for preparing an antibody capable of recognizing a protein that is specifically expressed on podocyte surface, to said antibody an insoluble carrier being bound; (2) a step for contacting the insoluble carrier-bound antibody with a urine specimen of a subject; and (3) a step for observing the urine specimen, which has been contacted with the insoluble carrier-bound antibody, under an optical microscope and counting podocytes.

Classes IPC  ?

• G01N 33/493 - Analyse physique de matériau biologique de matériau biologique liquide d'urine
• G01N 33/483 - Analyse physique de matériau biologique
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

The goal of the present invention is to provide a method for boosting sensitivity in an immunoassay system. The inventors perfected the present invention upon discovering that the aforementioned goal of boosting sensitivity in an immunoassay system can be achieved through pretreatment of the urine.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

Provided is a method which enables the highly efficient reverse transcription of a double-stranded RNA virus and also enables the amplification of the resultant reverse transcript. A method for synthesizing complementary DNA using double-stranded RNA as a template, said method being characterized in that a mixed solution of double-stranded RNA extracted from a sample and a primer is subjected to steps (1) and (2) as mentioned below in this order, and subsequently single-stranded DNA is synthesized from the mixed solution using a reverse transcriptase: (1) a step of heating the mixed solution at a temperature equal to or higher than 95˚C and then rapidly cooling the mixed solution; and (2) a step of heating the mixed solution at a temperature lower than 90˚C and then rapidly cooling the mixed solution.

Classes IPC  ?

• C12N 15/09 - Technologie d'ADN recombinant
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

Disclosed is a novel method for measuring hemagglutinin from an influenza virus, which enables the construction of a measurement system within a shorter period than a sandwich immunoassay method using two kinds of anti-hemagglutinin antibodies. The method for measuring hemagglutinin from an influenza virus is achieved by a sandwich immunoassay method comprising sandwiching the hemagglutinin with a lectin capable of binding to hemagglutinin but incapable of binding to an antibody and an anti-hemagglutinin antibody capable of undergoing an antigen-antibody reaction with the hemagglutinin.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

Disclosed is a method for the selective removal of triglycerides in lipoproteins other than low-density lipoproteins, in order to enable the provision of a method having excellent simplicity, specificity, and precision, and which is for direct LDL-TG fractional quantification and measurement of a sample, using an automatic analysis device, etc. without any complicated preprocessing such as centrifugation or electrophoresis etc. The method for the removal of triglycerides in lipoproteins other than low-density lipoproteins includes a step in which: lipoprotein lipase, cholesterol esterase, glycerol kinase, and glycerol-3-phosphate oxidase are caused to act on the sample, in the presence of a surfactant that acts on lipoproteins other than low-density lipoproteins or a surfactant having an LDL-protection action; and the generated hydrogen peroxide is removed.

Classes IPC  ?

• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
• C12Q 1/61 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des triglycérides
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Provided is a method for quantifying cholesterol in the whole HDL-C and HDL subfractions, i.e., ApoE-containing HDL-C and ApoE-deficient HDL-C, separately or simultaneously. A method for enzymatically quantifying cholesterol in an ApoE-deficient HDL in a selective manner by adding a surfactant comprising a polyoxyethylene benzyl phenyl ether derivative to a sample to be tested at a final concentration of 0.05 to 0.10%, allowing cholesterol esterase and cholesterol oxidase to act on the sample, and then quantifying generated hydrogen peroxide; and a method for enzymatically quantifying cholesterol in an ApoE-containing HDL in a selective manner by adding a surfactant comprising a polyoxyethylene benzyl phenyl ether derivative to a sample to be tested at a final concentration of 0.15 to 0.75%, allowing cholesterol esterase and cholesterol oxidase to act on the sample, and then quantifying generated hydrogen peroxide.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase

Abrégé

Disclosed are: an immunological analysis method which can measure an antigen with high sensitivity and high accuracy; and a reagent for use in the method. In the immunological analysis method, an antigen-antibody reaction and/or a measurement is carried out in the presence of a polycarboxylic acid-type surfactant. The immunological analysis reagent for use in the method is characterized by comprising a polycarboxylic acid-type surfactant. By employing such a simple means that a polycarboxylic acid-type surfactant is allowed to exist in a reaction and/or measurement system, it becomes possible to inhibit a non-specific reaction effectively in a high-sensitivity immunological analysis, whereby an antigen can be measured accurately and specificity can be improved in an immunological analysis.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Disclosed is the provision of a method for quantifying high density lipoprotein 3 (HDL3) in a test sample without requiring a troublesome procedure. A method for quantifying cholesterol in HDL3, said method comprising reacting a test sample with a surfactant that is capable of specifically reacting with HDL3 and thus quantifying cholesterol, wherein said surfactant is at least one substance selected from the group consisting of polyoxyethylene polycyclic phenyl ether and a polyoxyethylene styrene phenyl ether.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12Q 1/42 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une phosphatase
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase

Abrégé

Provided is a means for improving measurement accuracy in an immunoassay using a dry plastic cell. A method for preventing the adsorption of bubbles onto the side surface of a dry plastic cell in an immunoassay in which an antigen-antibody reaction is carried out in the cell using an immunoassay reagent capable of responding immunologically to an analyte of interest in a sample and the resultant reaction product is measured optically, said method being characterized in that the reaction and/or measurement is carried out in the presence of a surfactant.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is an anti-human-norovirus GII antibody capable of responding to substantially all genotypes of human norovirus belonging to GII and capable of comprehensively detecting human norovirus GII. An anti-human-norovirus GII antibody for binding to an epitope included in an amino acid region represented by formula (1) or (2) present in a P-domain of a capsid structural protein of human norovirus GII, and to one or more epitopes comprising the 483rd amino acid of an amino acid sequence represented by SEQ ID NO: 1 or an amino acid equivalent thereto. (1): P-X1-X2-P-G-E; (2): X3-X4-X5-F-Y-X6-L-X7-P-X8 (In the formulas, X1 represents L, V, N, T, S, M, or R; X2 represents F, Y, or M; X3 represents V or G; X4 represents N or S; X5 represents Q, P, or S; X6 represents S, T, or I; X7 represents A or S; and X8 represents M or V.)

Classes IPC  ?

• C07K 16/08 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

Disclosed is a method for quantifying HDL2 cholesterol in a sample of interest without requiring any complicated operation. A method for quantifying a cholesterol involves reacting an HDL with a phospholipase and quantifying the cholesterol. The method comprises a first step of allowing any cholesterol other than high-density lipoproteins contained in a sample of interest to transfer to the outside of a reaction system and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system. By performing the two steps in this manner, it becomes possible to quantify high-density lipoprotein 2 cholesterol in a sample of interest.

Classes IPC  ?

• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol

Abrégé

Provided are a method for simply and accurately quantifying a remnant-like lipoprotein in a sample, without requiring separation, and a kit for same. The method for quantifying a remnant-like lipoprotein in a sample containing a plurality of lipoprotein types, including a remnant-like lipoprotein, includes a step (1) for eliminating cholesterol in lipoproteins other than the remnant-like lipoprotein and a step (2) for quantifying the cholesterol in the remaining remnant-like lipoprotein. In the step (1), the sample is acted upon by a cholesterol esterase with a molecular weight of more than 40 kDa and which does not contain a subunit with a molecular weight of 40 kDa or less. In the step (2), the sample is acted upon by a cholesterol esterase with a molecular weight 40 kDa or less or a cholesterol esterase with a subunit with a molecular weight of 40 kDa or less.

Classes IPC  ?

• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/28 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une peroxydase
• C12Q 1/30 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une catalase
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Disclosed is a method for quantifying the amount of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring cumbersome procedures. Said method includes: a first step in which a test sample is acted on by a phospholipase and/or a sphingomyelinase and cholesterol is moved outside of the reaction system; and a second step in which the amount of cholesterol remaining in the reaction system is quantified. This makes it possible to use an automated analyzer to specifically quantify the amount of HDL3 cholesterol in a test sample without requiring cumbersome work such as ultracentrifuging and pretreatment. It is also possible to quantify the amount of HDL2 cholesterol by subtracting out the HDL3 cholesterol amount from a total HDL cholesterol amount obtained via an existing method for quantifying the total HDL cholesterol in a sample.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12Q 1/42 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une phosphatase
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Disclosed is a method for quantifying the amount of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring cumbersome procedures. Said method includes: a first step in which a test sample is reacted with a surfactant that reacts with lipoproteins other than high-density lipoprotein 3 and cholesterol is moved outside of the reaction system; and a second step in which the amount of cholesterol remaining in the reaction system is quantified. This makes it possible to use an automated analyzer to specifically quantify the amount of HDL3 cholesterol in a test sample without requiring cumbersome work such as ultracentrifuging and pretreatment. It is also possible to quantify the amount of HDL2 cholesterol by subtracting out the HDL3 cholesterol amount from a total HDL cholesterol amount obtained via an existing method for quantifying the total HDL cholesterol in a sample.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol

Abrégé

Disclosed is a method for quantifying the amount of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring cumbersome procedures. Said method includes: reacting a test sample with a surfactant that specifically reacts with high-density lipoprotein 3; and quantifying the amount of cholesterol. This makes it possible to use an automated analyzer to specifically quantify the amount of HDL3 cholesterol in a test sample without requiring cumbersome work such as ultracentrifuging and pretreatment. It is also possible to quantify the amount of HDL2 cholesterol by subtracting out the HDL3 cholesterol amount from a total HDL cholesterol amount obtained via an existing method for quantifying the total HDL cholesterol in a sample.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol

Abrégé

Disclosed is a method of preparing an immunogen and a method for producing an antibody that uses the immunogen, whereby an antibody can be produced even if an antigen is a hydrophobic small molecule such as cereulide. The method of preparing an immunogen comprises: a step for dissolving an antigen such as cereulide in a water-soluble organic solvent such as methanol; a step for mixing the antigen solution obtained with a carrier having antigenicity itself, such as dead Salmonella cells; and a step for vaporizing the water-soluble organic solvent. The method of preparing an immunogen is the first to provide an anti-cereulide antibody.

Classes IPC  ?

• C07K 11/02 - Depsipeptides ayant jusqu'à 20 amino-acides dans une séquence entièrement déterminéeLeurs dérivés cycliques, p. ex. valinomycines
• C07K 16/12 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de bactéries
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

Disclosed is a method for examining acute renal disorder characterized by comprising detecting podocalyxin in the urine. According to this examination method, a subject showing a urine podocalyxin level higher than a standard can be diagnosed as having acute renal disorder. Compared with the existing methods, the aforesaid examination method enables accurate and non-invasive diagnosis of acute renal disorder and, therefore, can reduce the physical burden on patients, which makes the method highly useful.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/70 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir la créatine ou la créatinine

Abrégé

Disclosed is a method wherein the cholesterol in the HDL subfractions of ApoE-containing HDL-C and ApoE-deficient HDL-C, or in all HDL-C can be either separately or simultaneously quantified. A surfactant selected from the following group is added to a test sample: a surfactant having an ApoE-containing HDL reaction rate/ApoE-deficient HDL reaction rate ratio which is at least 0.7 but less than 1.3, a surfactant having an ApoE-containing HDL reaction rate/ApoE-deficient HDL reaction rate ratio of less than 0.7, and a surfactant having an ApoE-containing HDL reaction rate/ApoE-deficient HDL reaction rate ratio of 1.3 or higher. Cholesterol esterase and cholesterol oxidase are allowed to act upon the resulting test sample, and by quantifying the hydrogen peroxide generated, the cholesterol in the ApoE-containing HDL and the cholesterol in the ApoE-deficient HDL are fractionally quantified using enzymes.

Classes IPC  ?

• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase

Abrégé

Disclosed is a test method for determining the necessity for renal biopsy in a subject who is suspected to have a renal disease. Specifically disclosed are: a test method on a renal disease, which is characterized by using a combination of podocalyxin contained in urine and at least one another marker; a test reagent for use in the test method; and a test reagent kit for use in the test method. The method, the reagent and the kit enable the distinction of a prognosis-poor group among from cases that are unprominent in poor prognosis in conventional test methods, and also enable the determination of occurrence of a renal disease, the determination of the necessity for renal biopsy, the prediction of prognosis or the like to be achieved accurately.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/493 - Analyse physique de matériau biologique de matériau biologique liquide d'urine
• G01N 33/70 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir la créatine ou la créatinine

Abrégé

Disclosed is a test method for detecting diabetic nephropathy in an earlier stage than conventional methods. Specifically disclosed are: a method for testing on diabetic nephropathy, which is characterized by measuring the urine podocalyxin level: the test method in which diabetic nephropathy is detected in at least the first stage; a test reagent for use in the test method; and a test reagent kit for use in the test method. The method, the test reagent and the test reagent kit rely on a fact that the urine podocalyxin level reflects the occurrence and condition of diabetic nephropathy highly sensitively in an earlier stage than the urine albumin level.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/493 - Analyse physique de matériau biologique de matériau biologique liquide d'urine
• G01N 33/70 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir la créatine ou la créatinine

Abrégé

Provided are a diagnostic kit and diagnostic marker for diagnosing kidney disease. This method for detecting kidney disease includes measurement of at least one of the following forms of human megalin present in urine: (i) full-length human megalin; (ii) intracellular domain fragments of human megalin missing the extracellular domains thereof; and (iii) extracellular domain fragments of human megalin missing the intracellular domains thereof.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères

Abrégé

Disclosed are: a convenient detection means for a renal disorder; a marker for diagnosing a renal disorder, in which megalin in urine observed in associated with a renal disorder and capable of being used as the detection means is measured to predict the prognosis of a renal disorder (e.g., diabetic renal nephropathy, IgA nephropathy) or evaluate the level of the severity of the disorder at the early stage of nephropathy; and use of the marker. Specifically disclosed is use of human megalin as a marker for detecting a renal disorder in urine collected from a subject.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

Disclosed is a simple device for membrane assay using the lateral flow immunoassay method, whereby a component to be detected can be detected at a high sensitivity, provided with, as a label-drying pad, a base material which has a higher tensile strength than glass fiber and can well release a label. Specifically disclosed is a simple membrane assay device, comprising: a supporting board; a sample-supply part; a label part containing a labeling component for labeling a subject to be detected; a development part provided with a detection part formed therein that comprises a trapping reagent for detecting or quantifying said subject to be detected; and an absorption part, characterized in that a non-woven fabric consisting of fibers having a fiber diameter of 0.05-10 μm is used in said label part.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Disclosed is a novel method whereby the measurement range in an immunoassay method can be broadened. An immunoassay method wherein a polyoxyethylene alkyl ether sulfate and a polyoxyethylene sorbitan fatty acid ester coexist in a reaction system. According to this method, scattering in, in particular, blank measurement values (standard deviation) can be controlled and thus the measurement values can be stabilized. Thus, a significant difference can be obtained even in measurement values in a low-concentration range wherein measurement can be hardly performed by the existing immunoassay methods and, therefore, the measurement accuracy in the low-concentration range can be improved.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

Described are a urine pretreatment agent, a urine pretreatment method, and a urinary protein quantitation method which reduce or cancel the influences of urine pH variations, cancel the influences of precipitates of urinary inorganic salts, and solubilize membrane proteins. The urine pretreatment agent for urinary protein quantitation, includes a buffer, a chelating agent, and a surfactant; the urine pretreatment method includes a step of mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and the urinary protein quantitation method includes steps of: mixing 10 to 1000 parts by mass of the urine pretreatment agent with 100 parts by mass of urine; and then measuring the protein concentration.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

Disclosed is a test reagent for an analyte in a test sample. The test reagent employs, as an indicator, the occurrence of the aggregation of particles in a particle-floating solution in which particles comprising insoluble carrier particles and an analyte-capturing substance supported on the insoluble carrier particles are floating, does not undergo self-aggregation during storage, and rarely undergoes non-specific aggregation during measurement. Also disclosed is a method for measuring an analyte in a test sample using the test reagent. Specifically disclosed is a test reagent for measuring an analyte, which comprises at least a solution (A) and a solution (B), wherein the solution (A) is a buffer solution having an electrical conductivity of 30 ms/cm or more and the solution (B) is a particle-floating solution in which particles comprising insoluble carrier particles and an analyte-capturing substance supported on the insoluble carrier particles are floating and has an electrical conductivity of 6.5 ms/cm or less.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

Disclosed is a means for separating/purifying an influenza virus antigen by removing any contaminant such as a host protein from an influenza virus culture solution with high efficiency in a simple manner. Specifically disclosed is a method for producing a purified influenza virus antigen, which comprises the following steps: treating a sample containing an influenza virus with a surfactant; contacting the sample produced after the proceeding treatment step with hydroxyapatite in the co-presence of the surfactant; and colleting a fraction that is not adsorbed on hydroxyapatite.

Classes IPC  ?

• C12N 7/02 - Isolement ou purification
• A61K 39/145 - Orthomyxoviridae, p. ex. virus de l'influenza
• A61P 31/16 - Antiviraux pour le traitement des virus ARN de la grippe ou des rhinovirus
• C07K 1/14 - ExtractionSéparationPurification
• C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
• C07K 14/11 - Orthomyxoviridae, p. ex. virus de l'influenza
• C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion

Abrégé

Disclosed is a method for inhibiting the adsorption of cystatin C onto a container in a simple manner to improve the accuracy of the measurement of cystatin C.  Specifically disclosed are: a cystatin C adsorption inhibitor comprising a nonionic surfactant; a cystatin C measurement reagent comprising the adsorption inhibitor; a cystatin C measurement kit; and a method for inhibiting the adsorption of cystatin C, which comprises contacting a sample containing cystatin C with a measurement instrument in the presence of a nonionic surfactant.  The nonionic surfactant is preferably a polyoxyethylene-type surfactant.  Alternatively, the nonionic surfactant preferably has a phenoxy structure, more preferably a benzylphenoxy structure.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

Disclosed is a test apparatus which displays quickly and clearly, is correct and stable, and is equipped with a reference display section that displays the correct accomplishment of the test. Specifically disclosed is a test apparatus for a membrane assay, which is characterized by utilizing the specific binding reaction among a capture reagent immobilized on a membrane carrier, a substance to be detected and a labeled reagent labeled with a labeling substance.  The test apparatus is also characterized by being equipped with a reference display section which has a cationic polymer immobilized thereon for capturing the labeled reagent and can display the correct accomplishment of the test.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Disclosed are a method and a reagent for the fractional measurement of a small, dense LDL, which enables the rapid, simple and highly-specific analysis without the need of any pre-treatment of a sample and which is applicable to an automatic analysis apparatus. Specifically disclosed is a method for quantifying a small, dense LDL cholesterol in a sample, which comprises the following steps (1) and (2): (1) scavenging any cholesterol in LDL other than the small, dense LDL in the presence of a phospholipase; and (2) measuring the quantity of a cholesterol in a lipoprotein which remains after the step (1).

Classes IPC  ?

• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/30 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une catalase
• C12Q 1/42 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une phosphatase
• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase

Abrégé

It is intended to develop a urine pretreatment agent which is capable of reducing or eliminating an effect of pH variation in urine, eliminating an effect of a deposit resulting from deposition of an inorganic salt in the urine, and solubilizing a membrane protein; a urine pretreatment method; and a urinary protein determination method. A urine pretreatment agent for urinary protein determination characterized by containing a buffer, a chelator, and a surfactant; a urine pretreatment method characterized by mixing the urine pretreatment agent in an amount of 10 to 1000 parts by mass based on 100 parts by mass of urine; and a urinary proteindetermination method characterized by mixing the urine pretreatment agent in an amount of 10 to 1000 parts by mass based on 100 parts by mass of urine and thereafter measuring a protein concentration are provided.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• G01N 33/493 - Analyse physique de matériau biologique de matériau biologique liquide d'urine
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

A method reducing a measurement error caused by the inhibition of a catalase by an azide that is to be used in a method of quantifying a target component comprising decomposing hydrogen peroxide originating in components other than the target component with a catalase. The method reducing a measurement error caused by the inhibition of a catalase by an azide comprises decomposing hydrogen peroxide originating in components other than the target component with a catalase, and then, in quantifying the target component by quantifying the hydrogen peroxide originating in the target component, using a catalase originating in a microorganism which has a subunit of 75 kDa or more as the catalase as described above.

Classes IPC  ?

• C12Q 1/30 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une catalase
• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol

Abrégé

It is intended to provide a safe and efficient method of producing a virus which is free from animal-origin components in the whole process from culturing adhesive cells to producing the virus on an industrial scale by the cell culture. Namely, a method of producing a virus comprising: adhering adhesive cells to a support which has a polypeptide (P) having at least one cell-adhesive minimum amino acid sequence (X) per molecule, and is free from animal-origin components; culturing the adhesive cells in a medium free from animal-origin components; subculturing the cultured adhesive cells using a cell dispersing agent: free from animal-origin components; and then inoculating and proliferating a virus in the cells obtained by culturing the adhesive cells.

Classes IPC  ?

• C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
• C07K 5/00 - Peptides ayant jusqu'à quatre amino-acides dans une séquence entièrement déterminéeLeurs dérivés

Abrégé

Disclosed is a reagent for the fractional determination of a small dense LDL, which does not need any pretreatment of a sample, is rapid and simple, and is applicable to an automated analyzer. Also disclosed is a method for the determination of the quantity of cholesterol contained in a small dense low-density lipoprotein in a sample, which comprises: a first step for scavenging a lipoprotein other than the small dense low-density lipoprotein in the sample in the presence of cholesterol esterase and a surfactant capable of acting on a lipoprotein other than the small dense low-density lipoprotein at a concentration of 0.05 to 1.0 g/L; and a second step for determining the quantity of cholesterol in the small dense low-density lipoprotein remaining in the first step.

Classes IPC  ?

• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/30 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une catalase
• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Disclosed are: a precipitated/inactivated whole virus influenza vaccine which can effectively act on a new-type influenza virus, particularly a new-type virus having low immunogenicity, at a low amount of antigen and which has little adverse side-effects; and a method for producing the vaccine. Specifically disclosed are: a precipitated/inactivated influenza vaccine comprising an aluminum hydroxide gel prepared from sodium carbonate and aluminum potassium sulfate and an isolated whole influenza virus particle as active ingredients; and a method for producing the precipitated/inactivated influenza vaccine, which is characterized by conducting each step by using a solvent containing no surfactant or no ether.

Classes IPC  ?

• A61K 39/145 - Orthomyxoviridae, p. ex. virus de l'influenza
• A61K 39/39 - Préparations médicinales contenant des antigènes ou des anticorps caractérisées par les additifs immunostimulants, p. ex. par les adjuvants chimiques
• A61P 31/16 - Antiviraux pour le traitement des virus ARN de la grippe ou des rhinovirus

Abrégé

Disclosed are: an antibody directed against Panton-Valentine leukocidin (PVL) toxin derived from Staphylococcus aureus; a method and a kit for the detection of the toxin using the antibody; and a pharmaceutical composition for the treatment of PVL-carrying Staphylococcus aureus, which comprises an antibody directed against Panton-Valentine leukocidin toxin. Specifically, disclosed are: an antibody capable of binding to Panton-Valentine leukocidin F and having no cross-reactivity with LukD and/or HlgB; and an antibody capable of binding to Panton-Valentine leukocidin S and having no cross-reactivity with at least one member selected from LukE, HlgC and HlgA.

Classes IPC  ?

• C07K 16/12 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de bactéries
• A61K 39/09 - Streptococcus
• A61K 39/395 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire
• A61P 31/04 - Agents antibactériens
• A61P 37/04 - Immunostimulants
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

It is intended to disclose a latex composition for immunoassay whereby the spontaneous agglutination of latex particles to which an antibody is bound is prevented so as to enable highly sensitive immunoassay; and an immunoassay method, an immunochromatography apparatus and a flow-through immunoassay apparatus with the use of the latex composition as described above. This latex composition for immunoassay comprises latex particles to which an antibody or a fragment thereof capable of binding to an antigen is bound, a medium in which the latex particles are suspended, at least one agglutination inhibitor selected from the group consisting of a saccharide and a polyhydric alcohol, a protein and a buffering agent and has a pH value of from 9.0 to 9.8.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

The object is to provide an excellent method for detection/diagnosis of familial combined hyperlipemia. Disclosed is a method for detection of familial combined hyperlipemia, which comprises determining the small, dense LDL cholesterol level in a sample collected from a subject.

Classes IPC  ?

• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

The object is to provide: a human megalin determination method which is simpler and can be achieved within a shorter time compared to a conventional method, and can also determine human megalin quantitatively; and a diagnosis method which can diagnose a cell-, tissue- or organ-specific functional disease directly in an affected site at an early stage of the disease. Disclosed is a method for detection of an organ disease in which the expression of megalin is characteristically observed, by means of determining human megalin.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
• C12N 15/09 - Technologie d'ADN recombinant

Abrégé

It is intended to provide an immunochromatographic detection device which detects PBP2’ specifically produced by a multidrug-resistant Staphylococcus bacterium by an immunochromatographic detection method sensitively, simply and rapidly and is capable of determining infection with a multidrug-resistant Staphylococcus, a diagnostic method using the detection device, and a diagnostic kit including the detection device. The invention is directed to the immunochromatographic detection device for detecting a multidrug-resistant Staphylococcus which includes a sample supply unit which supplies a sample solution which is assumed to contain a multidrug-resistant Staphylococcus or a solution which is assumed to contain PBP2’ released from a cell wall by a pretreatment of a sample onto a sheet-shaped solid-phase support, a labeling reagent unit which retains a labeling reagent in which an antibody specifically binding to PBP2’ is labeled spreadable on the solid-phase support, and a trapping reagent unit in which a trapping reagent capable of trapping a complex of PBP2’ and the labeling reagent by specifically binding to the complex is immobilized, and in which an amphoteric surfactant, an anionic surfactant and/or a nonionic surfactant is contained in the trapping reagent unit or in the solid-phase support upstream of the trapping reagent unit.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/531 - Production de matériaux de tests immunochimiques

Abrégé

Disclosed is a method for determining a serotype rapidly in a simple manner. An immunoagglutination assay comprises mixing a bacterial cell with an antibody in an aqueous solvent and irradiating the mixed solution with light to optically determine the agglutination of the cell caused by antigen-antibody reaction.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

Disclosed is a selective quantification method which can simultaneously quantify two substances by one measurement run and comprises two steps composed of a first step and a second step, wherein one reagent is used in each of the steps. The method can quantify two substances in a biological sample simultaneously by using two reagents, wherein a reaction product of a first substance is detected in the initial stage of the second step and a reaction product of a second substance is detected subsequently.

Classes IPC  ?

• C12Q 1/60 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le cholestérol
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/61 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des triglycérides
• G01N 21/78 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique produisant un changement de couleur
• G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur
• G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol

Abrégé

Disclosed is a method for performing separation/determination of a small, dense LDL rapidly in a simple manner without the need of any pre-treatment of a sample, which is applicable to an automatic analyzer. A method for quantification of a small, dense LDL cholesterol comprising the steps of mixing an enzyme for determination of cholesterol with a sample in the presence of a polyoxyethylene-polyoxypropylene copolymer or a derivative thereof to allow the copolymer or derivative to react with the small, dense LDL selectively, among other lipoproteins, and determining the amount of cholesterol produced by the reaction.

Classes IPC  ?

• C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
• C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
• C12Q 1/32 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une déshydrogénase