A recombinant glucose dehydrogenase as well as a preparation method therefor and the use thereof. Specifically, the present invention comprises using genetically engineered Escherichia coli to express the recombinant glucose dehydrogenase. Soluble expression of the recombinant glucose dehydrogenase in the Escherichia coli system enables an expressed target protein to have high yield and high catalytic activity. Therefore, the present invention has the advantages of short production period, easy purification of expression products, low production cost and the like, and can realize industrial production of the recombinant glucose dehydrogenase.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
Provided is a method for preparing formamidopyrimidine DNA glycosylase. Specifically, recombinant formamidopyrimidine DNA glycosylase is expressed by means of genetically engineered Escherichia coli. The recombinant formamidopyrimidine DNA glycosylase achieves high-level soluble expression in the Escherichia coli system, and has strong catalytic activity. Thus, the present invention has the advantages of short production cycle, easy purification of expression products, low cost and the like, and can achieve industrial production of recombinant formamidopyrimidine DNA glycosylase.
A recombinant DNA polymerase for sequencing is provided. The recombinant DNA polymerase is a mutant of a 9N° DNA polymerase, the 9N° DNA polymerase mutant can recognize a 3-O-azido-modified reversible terminator and keep high incorporation activity, so that said mutant can be used for second-generation sequencing. The present invention uses a genetically engineered Escherichia coli strain for recombinant expression of the 9N° DNA polymerase mutant, and the expressed mutant enzyme realizes soluble expression in an Escherichia coli system, and therefore has high catalytic activity.
Provided are a preparation method and use for a truncated body of a Bst DNA polymerase mutant. The provided truncated body of the Bst DNA polymerase mutant has good heat resistance and can be stably expressed in an E. coli system. The provided preparation method for the truncated body of the Bst DNA polymerase mutant can express a large amount of soluble Bst DNA polymerase.
The present application belongs to the technical field of chemiluminescent immunoassays and relates to a single-person reagent strip. The single-person reagent strip comprises a bottom plate and reagent cup sets; magnetic rod sleeving hole positions, a magnetic bead accommodation recess, at least two sets of reagent holes, and at least two mounting slots are provided on the bottom plate; and the reagent cup sets are detachably mounted in the mounting slots, each reagent cup set comprising a fixing plate and at least two reagent cups, the reagent cups being provided on the fixing plate, and the number of reagent cups corresponding to the number of reagent holes. In the technical solution provided in the present application, combined testing of a plurality of items can be carried out on one reagent strip according to medical testing requirements; testing efficiency is improved, and there is no interference between test items.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
An implantable biosensor (100) and a preparation method therefor. The implantable biosensor (100) comprises a flexible carrier (1), a sensing element (2), a signal output end (3), and a transmitter (4). The flexible carrier (1) extends in a first direction and comprises a sensing section (11) and an output section (12) connected to each other in the first direction; the sensing element (2) is located on the surface of the sensing section (11) and is operable to react with a target biomolecule and generate a signal; the signal output end (3) is connected to the output section (12) and is electrically connected to the sensing element (2); the transmitter (4) is provided with a signal receiving end (45); the signal receiving end (45) is electrically connected to the signal output end (3) by means of a flexible connecting assembly (5); and the flexible connecting assembly (5) has elasticity.
Provided are a primer-probe set, a test kit and a method for detecting influenza A H1N1 virus, which relate to the technical field of molecular detection. The primer-probe set comprises: an HA forward primer, having a nucleotide sequence as shown in SEQ ID NO. 1; an HA reverse primer, having a nucleotide sequence as shown in SEQ ID NO. 2; an HA probe, having a nucleotide sequence as shown in SEQ ID NO. 3; an NA forward primer, having a nucleotide sequence as shown in SEQ ID NO. 4; an NA reverse primer, having a nucleotide sequence as shown in SEQ ID NO. 5; an NA probe, having a nucleotide sequence as shown in SEQ ID NO. 6; an internal control forward primer, having a nucleotide sequence as shown in SEQ ID NO. 7; an internal control reverse primer, having a nucleotide sequence as shown in SEQ ID NO. 8; and an internal control probe, having a nucleotide sequence as shown in SEQ ID NO. 9. Detection results are accurate, and have high sensitivity.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
The present application belongs to the technical field of medical testing and relates to a microfluidic testing device and a nucleic acid testing apparatus. The microfluidic testing device comprises a housing, which is internally provided with a functional chamber, a first sample injection assembly, a second sample injection assembly, fluid quantification pools, capillary adsorption members and a result display assembly, wherein the first sample injection assembly and the second sample injection assembly are both in communication with the functional chamber; the functional chamber is in communication with the fluid quantification pools; the fluid quantification pools are in communication with the result display assembly by means of the capillary adsorption members; and the result display assembly is configured to display a test result. The water absorption amount of the result display assembly is controlled by means of the fluid quantification pools, and liquids in the fluid quantification pools are slowly chromatographed to the result display assembly by means of the capillary adsorption members, thereby achieving the effect of accurately controlling the water absorption amount on a test strip.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
9.
PRIMER PROBE GROUP, KIT AND METHOD FOR DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA
The application belongs to the technical field of mycobacterium tuberculosis and nontuberculous mycobacteria nucleic acid detection, and relates to a primer probe group, kit and method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria. The primer probe group comprises: an upstream primer for a mycobacterium tuberculosis complex group specific gene conserved region; a downstream primer for the mycobacterium tuberculosis complex group specific gene conserved region; a upstream primer for a nontuberculous mycobacteria specific gene conserved region; a downstream primer for the nontuberculous mycobacteria specific gene conserved region; an upstream primer for an internal standard gene; a downstream primer for the internal standard gene; a probe for the mycobacterium tuberculosis complex group specific gene conserved region; a probe for the nontuberculous mycobacteria specific gene conserved region; and a probe for the internal standard gene. The present solution has a high specificity, mycobacterium tuberculosis and nontuberculous mycobacteria mixed infection can be detected, and mycobacterium tuberculosis and nontuberculous mycobacteria can be distinguished and identified.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
Provided are primer-probe sets, a kit and a method for detecting Y chromosome microdeletion sites. The primer-probe sets comprise an SY88 primer-probe set, an SY10645 primer-probe set, an SY1065 primer-probe set, an SY105 primer-probe set, an SY153 primer-probe set, an SY81 primer-probe set, an SY121 primer-probe set, an SY145 primer-probe set, an SY154 primer-probe set, an SY182 primer-probe set, an SY1245 primer-probe set, an SY82 primer-probe set, an SY84 primer-probe set, an SY86 primer-probe set, an SY127 primer-probe set, an SY128 primer-probe set, an SY143 primer-probe set, an SY239 primer-probe set, an SY255 primer-probe set, an SY254 primer-probe set, an SY133 primer-probe set, an SY152 primer-probe set, an SY134 primer-probe set, an SY242 primer-probe set, an SRY primer-probe set, and an internal standard gene GAPDH primer-probe set, wherein each set of the primer-probe sets comprises its corresponding primer and probe.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
An isothermal amplification detection device, comprising a temperature control platform (100), a reaction platform (200), a plurality of first heat conduction members (300), and a plurality of second heat conduction members (400). The temperature control platform (100) is provided with a phase change temperature control cavity (110); the phase change temperature control cavity (110) is used for containing a phase change material; the reaction platform (200) is connected to the temperature control platform (100); the reaction platform (200) is provided with at least one reaction cavity (210) used for accommodating a liquid for reaction; the first heat conduction members (300) and the second heat conduction members (400) are all provided in the phase change temperature control cavity (110); the first heat conduction members (300) are in heat conduction connection with a heat source, so as to conduct heat of the heat source into the phase change temperature control cavity (110); and the second heat conduction members (400) are connected to the reaction platform (200), so as to conduct heat of the phase change material in the phase change temperature control cavity (110) into the reaction cavity (210). The device can effectively improve the heat conduction efficiency.
The present invention relates to the technical field of nucleic acid detection. Disclosed is a microfluidic chip detection card box, comprising a housing (1), the housing (1) being provided with a functional chamber (2), a first flow channel assembly (3), a second flow channel assembly (4), and a result display assembly (5); both the first flow channel assembly (3) and the second flow channel assembly (4) are in communication with the functional chamber (2), the functional chamber (2) is in communication with the result display assembly (5), and the result display assembly (5) is used for displaying a detection result. According to the microfluidic chip detection card box, nucleic acid amplification sample adding and diluent sample adding are carried out separately in different flow channels, so that reagent consumption is low, and reagent waste can be reduced; by means of the first flow channel assembly (3) and the second flow channel assembly (4), the nucleic acid amplification sample adding and reaction liquid dilution are carried out separately, thereby realizing accurate control of sample adding and detection, and having the advantages of simple operation, safety, reliability, high detection efficiency, high sensitivity, and low sample consumption; the nucleic acid amplification sample adding can be used to detect many different biological samples, thereby having a very wide application range.
A handheld nucleic acid testing device having a micro-fluidic chip, and a use method therefor, relating to the technical field of micro-fluidic chips. The handheld nucleic acid testing device having a micro-fluidic chip comprises: a sample collection tube (1); a chip connector (2) provided with a puncture tube (21) used for puncturing the sample collection tube (1); and a testing chip (3) connected to the chip connector (2) and provided with a plurality of chambers (33) and a plurality of flow channels (34), the flow channels (34) being communicated with the puncture tube (21) and the chambers (33). A reaction reagent and all reaction processes are concentrated and completed on one chip, operations such as liquid injection and liquid transfer are performed without using an external instrument, and nucleic acid testing can be performed without professional experimental places and special conditions. The handheld nucleic acid testing device having a micro-fluidic chip can be popularized and applied in community hospitals, family self-testing, public places and the like, and achieves real-time screening and testing in the epidemic prevention and control period and self-testing of personal health conditions.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
14.
TAQ ENZYME REVERSIBLE MODIFIER, CHEMICALLY MODIFIED TAQ ENZYME, AND PREPARATION METHOD THEREFOR AND PCR KIT THEREOF
A Taq enzyme reversible modifier, a chemically modified Taq enzyme, and a preparation method therefor and a PCR kit thereof. The structure of the Taq enzyme reversible modifier is as shown in formula (I). The activity of the Taq enzyme modified with the Taq enzyme reversible modifier is better than the activity of the Taq enzyme modified with N-ethoxycarbonyl-2,3-disubstituted maleimide.
A method for improving the activity of a chemically modified Taq enzyme and a Taq enzyme treated using same. The method comprises the steps of performing high performance liquid chromatography on a prepared crude product solution of a chemical modifier and carrying out a modification reaction with a Taq enzyme to be modified. The method for improving the activity of a chemically modified Taq enzyme can greatly improve the activity of a chemically modified Taq enzyme, and in particular the activity of a Taq enzyme modified with the compound represented by formula (I) in a low-cost, simple and convenient way.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12N 9/99 - Inactivation des enzymes par traitement chimique
Provided are a Taq polymerase mutant, and a preparation method therefor and the use thereof. The Taq polymerase mutant includes an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO: 2, wherein the amino acid sequence has mutations occurring at one or more positions selected from the following groups: P40, L125, G200, D335, G499, E634, F769 or a combination thereof. The provided Taq polymerase mutant has high amplification activity, can obtain more amplification products compared with wild-type Taq polymerase under the same number of PCR cycles, and moreover, is resistant to whole blood and high salt and has low 5'-3' exonuclease activity.
Provided are a Taq enzyme mutant, a preparation method, and an application thereof. The Taq enzyme mutant comprises D355, G499, E634, and F769. The Taq enzyme mutant has high amplification activity. Under the same PCR cycle times, the Taq enzyme mutant can obtain more amplification products as compared with wild-type Taq enzymes. Furthermore, the Taq enzyme mutant is resistant to whole blood and salts, and does not lose 5'-3'exonuclease activity.
The present invention relates to the technical field of high-throughput sequencing, and relates to a high-throughput sequencing method based on an internal reference of a known tag, comprising: generating a random DNA sequence, adding a single-ended linker DNA sequence containing the known tag at the two ends of the DNA sequence so as to obtain an internal reference sequence, and on the basis of the internal reference sequence, synthesizing a sequencing quality control sequence; by means of the sequencing quality control sequence, performing high-throughput sequencing on a sample library to be detected, so as to obtain sequencing data; and performing result analysis on the sequencing data to obtain a sample error allocation rate of the sample library to be detected, and ending the high-throughput sequencing. The present application can monitor a tag hopping condition in a sequencing process by means of the internal reference.
Provided in the present invention is a method for preparing a constant-temperature amplification mixed enzyme system. Specifically, disclosed in the present invention is a method comprising: removing the vast majority of glycerol from a prepared glycerol-containing RPA enzyme system by means of dialysis, and then concentrating the enzyme system containing trace glycerol by using an ultrafiltration method. Experiments show that the amplification performance of an RPA mixed enzyme system prepared by means of such method is significantly improved.
Provided are a reverse-transcription amplification system and method based on recombinase polymerase amplification (RPA) technology. Specifically, provided are that an RNA template in a sample can be effectively amplified after a reverse transcriptase and RNasin are added to an RPA system, and on this basis, the nucleic acid amplification efficiency of a reverse-transcription real-time fluorescent RPA system (RT-qRPA) can be effectively improved by means of adding an inorganic pyrophosphatase (IPP).
A kit for detecting a SARS-CoV-2 antigen and a detection method therefor. The kit comprises: a detection card (10), wherein a binding pad (12) and a reaction pad (13) are arranged on the detection card (10), labelling antibody Ab1 and labeling antibody Ab3 of a SARS-CoV-2 antigen are provided on the binding pad (12), a quality control line (16) and a detection line (17) are provided at intervals in parallel on the reaction pad (13), coating antibody Ab2 is provided on the detection line (17), and coating antibody Ab4 is provided on the quality control line (16). In the kit, an AIE fluorescent immunochromatography technology is used, an AIE fluorescent microsphere is used as a marker, and detection results of the detection card (10) are directly read using a fluorescent flashlight, or the detection results are read using a fluorescence detector. This kit has the advantages of high sensitivity, high specificity, simple and fast operation, etc.
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
G01N 33/558 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant la diffusion ou la migration de l'anticorps ou de l'antigène
G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
22.
PROTEIN STABILIZER, REAGENT TEST KIT AND PROTEIN PROTECTION METHOD
Provided are a protein stabilizer, a reagent test kit, and a protein protection method. The protein stabilizer comprises: 1-10 parts by weight of bovine serum albumin (BSA); 0.1-5 parts by weight of a nonionic surfactant; 5-40 parts by weight of polyol; 1-10 parts by weight of other additives; and 35-92.9 parts by weight of water; wherein, the other additives are selected from at least one of: a saccharide additive, polyvinylpyrrolidone and carbamide. The provided protein stabilizer has a simple formula, safe components, low manufacturing cost and a wide range of applications; and protein to which has been added the protein stabilizer of the present invention can be stored at room temperature, thus avoiding repeated freezing/thawing and sub-packaging, and is thus convenient to use.
C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
The present invention relates to the technical field of medical examination and determination, and relates to an in-vitro diagnostic reagent preservative, comprising a composition of a sulfadoxine solution and a diaveridine solution, wherein the molar ratio of sulfadoxine to diaveridine in the diaveridine solution is 0.002-1. The present application further relates to a use of the in-vitro diagnostic reagent preservative.
C12Q 1/52 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase une transaminase
C12Q 1/32 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une déshydrogénase
C12Q 1/61 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des triglycérides
C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
C12Q 1/28 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une peroxydase
24.
LIQUID-BASED CELL PRESERVATION SOLUTION AND PREPARATION METHOD THEREFOR
The present application discloses a liquid-based cell preservation solution and a preparation method therefor. The preservation solution comprises according to the percentage by mass: 15-20% of alcohol, 0.4-0.7% of buffer component, 0.2-0.5% of ionic strength regulator, 0.05-2% of anticoagulant, 0.05-0.2% of mucus dissociation agent, 0.5-0.9% of preservative, and the remainder being purified water. The cell preservation solution is reasonable in component setting, and can preserve cells at room temperature for a long time and maintain cell morphology; the addition of the mucus dissociation agent can effectively soften cell mucus, such that cells adhered by the mucus in a sample can be separated out, and cell accumulation can be avoided; and the addition of the preservative can effectively inhibit the proliferation of mould, bacteria, or other pathogens, and is beneficial to cell preservation.
The present application relates to the field of biomedical treatment. Disclosed is a sub-packaging device for material balls. The sub-packaging device for the material balls of the present application comprises: a feeding pipeline which is arranged vertically, wherein the feeding pipeline is provided with a feeding pipeline inlet at the top and a feeding pipeline outlet at the bottom; a sub-packaging portion including a housing and a drum rotatably arranged in the housing, wherein an outer peripheral surface of the drum is provided with a plurality of sub-packaging accommodation portions which are spaced apart circumferentially, the housing is provided with a top opening and a bottom opening, and the top opening is in communication with the feeding pipeline outlet; and a drying portion, wherein the feeding pipeline and the sub-packaging portion are arranged in a drying cavity of the drying portion. The sub-packaging device for the material balls of the present application realizes automatic sub-packaging of material balls, solves the defect of same being separately sub-packaged in a traditional manual manner, and has the advantages of being easy to operate, highly efficient and capable of achieving automation; and at the same time, the drying cavity is arranged in the sub-packaging device, such that the problems that during a sub-packaging process, shrinkage and wall adhesion caused by moisture absorption due to the fact that the material balls have been in contact with the air for a long time are avoided.
The present application relates to the field of biomedical treatment. Disclosed is an automated packaging system, comprising: a first screening module, comprising a feeding part and a screening pipeline, the screening pipeline being obliquely arranged, the top of the screening pipeline being communicated with a feeding outlet of the feeding part, and a screen being arranged on the lower side of the screening pipeline; a second screening module, comprising a storage part and a screen cylinder, an inlet of the storage part being communicated with the bottom of the screening pipeline; a dispensing and packaging module, comprising a dispensing and packaging pipeline and a dispensing and packaging cylinder, an inlet of the dispensing and packaging pipeline being communicated with the bottom of the screen cylinder, the dispensing and packaging cylinder comprising a housing and a rotating cylinder in the housing, a plurality of circumferentially spaced dispensing and packaging slots being provided on the periphery of the rotating cylinder, and the top opening of the housing being communicated with an outlet of the dispensing and packaging pipeline; and a film sealing module, comprising a film sealing machine and a carrying device, a hot pressing device being arranged above an internal channel of the film sealing machine. The hot pressing device moves up and down in the internal channel to perform hot pressing of film sealing, and the carrying device is located below the bottom opening of the housing and can pass through the internal channel. The carrying device is provided with a temperature control part. The present application realizes automated material screening, dispensing, packaging and film sealing.
Provided is a method for constructing a DNA next-generation sequencing library, the method comprising the steps of: enzyme cleavage, end repair and A tailing of genomic DNA; joint connection of DNA fragments; magnetic bead purification treatment of a product after joint connection; and PCR amplification of DNA fragments, and selection and purification treatment of fragments of a PCR product. In the method for constructing a DNA next-generation sequencing library, by using a dual enzyme cleavage and end repair one-step reaction process, fragments are fragmented using VVN and T7, a 5' protruding terminus is subjected to blunting via polymerization with Taq DNA polymerase, and A (adenine) tailing is then performed on a 3' terminus, thus realizing the integration of a one-step reaction for constructing a DNA next-generation sequencing library. At the end of the first-step reaction, magnetic bead purification is not required; and in the preparation process, two restriction endonucleases have no preference, and the sequencing of target fragments can be realized.
Provided are a method for constructing an RNA and DNA next-generation sequencing library, and a next-generation sequencing kit. The method for constructing the next-generation sequencing library comprises the following steps: performing first strand synthesis on RNA and DNA to obtain first strand cDNA; performing second strand synthesis on the first strand cDNA to obtain a second strand cDNA; performing fragmentation, end repair, phosphorylation and A addition treatment on the second strand cDNA to obtain an A-added product; performing linker linking and first purification treatment on the A-added product to obtain a target RNA fragment and a DNA fragment, and recovering same; and performing a PCR amplification reaction on the target RNA fragment and the DNA fragment so as to construct and obtain the RNA and DNA next-generation sequencing library. In the method, the next-generation sequencing library is constructed, the overall library building procedure is used, and process steps are simplified, such that operation procedure and experiment time are greatly shortened, and the loss of nucleic acid samples can be reduced to the maximum extent; meanwhile, the construction of two types of libraries is not required, such that the costs of manpower, reagents, time, etc. can be greatly saved.
A material ball screening device, comprising: a feeding portion, wherein the feeding portion is provided with a feeding cavity, and the feeding cavity is provided with a feeding port at the top and a discharging port at the bottom; a screening pipeline which is obliquely arranged, wherein the top of the screening pipeline is in communication with the discharging port of the feeding portion, and at least part of a lower side of the screening pipeline is provided with a screening member; and a drying portion, wherein the feeding portion and the screening pipeline are arranged in a drying cavity of the drying portion. The material ball screening device can achieve the purpose of automatic screening, and can avoid the problems of shrinkage and wall adhesion caused by the moisture absorption of material balls.
The present application relates to the biomedical field, and discloses a material screening apparatus. The material screening apparatus of the present application comprises: a screening column portion, wherein a screening column cavity is formed in the screening column portion, and the screening column cavity is provided with a screening column inlet at the top and a screening column outlet at the bottom; a feeding pipeline, wherein the feeding pipeline is vertically arranged, and the top of the feeding pipeline is communicated with the screening column outlet of the screening column portion; a drying portion, wherein the screening column portion and the feeding pipeline are arranged in a drying cavity of the drying portion; and at least one air injection apparatus, wherein the at least one air injection apparatus is provided with at least one air injection port located in the screening column cavity. The material screening apparatus of the present application can achieve the purpose of automatic screening, and avoid materials from shrinking or adhering to a wall due to moisture adsorption.
The present application relates to the field of biomedical treatment, and discloses a film sealing device. In the present application, the film sealing device comprise: a film sealing machine provided with a conveying passage, a film pressing device being arranged above the conveying passage, and the film pressing device being used for moving up and down in the conveying passage to perform hot pressing for film sealing; a conveying device configured to be movable through the conveying passage; and a loading unit arranged on the conveying device, wherein at least a part of the conveying device is provided with a constant temperature portion. In the present application, the film sealing device avoids damage to a film sealing sample caused by temperature rise after the film sealing machine works for a long time.
A purification method for fluorescein-labeled nucleoside triphosphate, specifically comprising: purifying a fluorescein-labeled nucleoside triphosphate crude product by means of liquid chromatography, and collecting a target fraction, wherein a mobile phase comprises a first component and a second component, the first component is an aqueous n-hexylamine acetate solution, and the second component is selected from acetonitrile or methanol. According to the purification method, the nucleoside diphosphate in a fluorescein-labeled nucleoside triphosphate crude product which has a similar structure to nucleoside triphosphate and is difficult to separate can be separated from a target product, thereby obtaining fluorescein-labeled nucleoside triphosphate having a purity of greater than 99%. The purified fluorescein-labeled nucleoside triphosphate has good fluorescence intensity and high sensitivity, and meets the requirements of fluorescence in-situ hybridization experiments.
A method and a kit for constructing a fluorescent oligonucleotide standard curve. The method comprises: step 1, using an aqueous solution of ammonia to formulate a standard solution of fluorescent oligonucleotide. The fluorescent oligonucleotide standard curve constructed by the method has good linearity and can be used for measuring the background fluorescence intensity of a TaqMan probe with an extremely low response value.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
34.
BACTERIA DETECTION SYSTEM AND BACTERIA DETECTION METHOD
GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES (Chine)
Inventeur(s)
Jiang, Xiwen
Zhang, Tianyu
Liu, Yue
Lu, Zhili
Cai, Xiaoyin
Abrégé
Disclosed in the present invention are a bacteria detection system and a bacteria detection method. The bacteria detection system comprises a control module, a mobile module, a drug susceptibility plate fixing apparatus and a detection module, the drug susceptibility plate fixing apparatus being used to fix a drug susceptibility plate holding bacteria to be detected; the detection module comprises a detection apparatus and a light shielding apparatus, the detection apparatus being disposed above the drug susceptibility plate fixing apparatus and used to detect luminescence of bacteria contained in the drug susceptibility plate, and the light shielding apparatus being disposed at a bottom of the detection apparatus and preventing external light from entering the detection apparatus; the mobile module is connected to the detection apparatus and drives the detection apparatus to move; and the control module is electrically connected to the mobile module and the detection apparatus to control actions of the detection apparatus and the mobile module. The present invention targets deficiencies of bacteria detection apparatuses in the prior art, adding a light shielding apparatus to reduce interference of an external light source toward a detection process, thereby ensuring more accurate mycobacterial detection results.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12Q 1/18 - Test de l'activité antimicrobienne d'un matériau
C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
35.
METHOD AND KIT FOR MULTIPLE DETECTION OF RESPIRATORY VIRUS NUCLEIC ACIDS
Provided in the present invention are a method and a kit for multiple detection of respiratory virus nucleic acids, and in particular, disclosed are a method, a primer, a probe and a kit for detecting a plurality of influenza A viruses such as H1N1(2019), H3N2, H5N1, H1N1 and H7N9, influenza B viruses such as Yamagata and Victoria, 2019-nCoV OFR1ab and N genes, and human internal standard gene GAPDH on the basis of a real-time fluorescent quantitative PCR technical platform.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
Disclosed are a swab sample nucleic acid releaser and an application thereof. The sample releaser comprises 1-20 mol/L of metal ion chelating agent, 5-80 mmol/L of buffer solution with a pH value of 6-9, 5%-40% (v/v) of polar organic solvent, 0.2%-10% (v/v) of surfactant and 0.1-1 mol/L of nuclease inhibitor. The sample releaser may integrate three steps of sampling preservation, deactivation and nucleic acid extraction into one step, and the whole operation process from sampling to PCR detection is free from heating and midway lid opening, and operation is therefore simple.
The present invention provides a novel coronavirus rapid detection kit based on thermal convection PCR and a method for multiple detection of novel coronavirus nucleic acid. The kit comprises a combination of primers and probes for detecting N genes and ORF1 ab genes of a novel coronavirus genome.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
Disclosed is a preservation tube for a sampling swab, the preservation tube comprising a tube body (1), wherein the tube body (1) is internally provided with a first cavity (11) for storing a sample releasing agent and a second cavity (12) for having a sampling swab placed therein, the first cavity (11) being arranged in communication with the second cavity (12), and the central axis of the second cavity (12) deviating from the central axis of the tube body (1). When in use, the sampling swab is placed in the second cavity (12) and is moved up and down and rotated in the second cavity (12) for elution, and a nucleic acid sample of the sampling swab is uniformly distributed in the sample releasing agent. The sample releasing agent in the first cavity (11) can be drawn for testing, thereby omitting the process of extracting the nucleic acid sample by means of oscillating elution. The swab is vertically placed in the second cavity (12) deviating from the center of the tube body (1), thereby providing an operation space for a sampling operation, simplifying a nucleic acid testing operation process, and facilitating a clinical test operation.
A61B 10/00 - Instruments pour le prélèvement d'échantillons corporels à des fins de diagnostic Autres procédés ou instruments pour le diagnostic, p. ex. pour le diagnostic de vaccination ou la détermination du sexe ou de la période d'ovulationInstruments pour gratter la gorge
39.
HEAT-RESISTANT DNA POLYMERASE MUTANT HAVING HIGH AMPLIFICATION ACTIVITY
A heat-resistant DNA polymerase mutant having high amplification activity. By using a protein directed evolution technology, a random mutation library is constructed for a polymerase activity structural domain of Taq enzyme. By gradually adding screening pressure, unsuitable mutations are naturally eliminated, whereas mutations having dominant traits are gradually accumulated. Finally, a series of amino acid sites which have key effects on Taq enzyme amplification performance and polymerization performance, as well as mutations thereof are obtained by means of screening, and further Taq enzyme mutants having high amplification performance are obtained.
The present invention provides an oligonucleotide conjugate having high hybridization performance and an application thereof. The oligonucleotide conjugate of the present invention can be used as a probe of nucleic acid, and by using the oligonucleotide conjugate of the present invention, the probe can be more flexibly designed, and a conserved region is more easily obtained. The specificity of the probe is better, a fluorescence value of the probe can be increased, the fluorescence background is reduced, and the sensitivity of the probe is improved.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
41.
RT-PCR DETECTION METHOD AND KIT FOR NOVEL CORONAVIRUS
A real-time fluorescence RT-PCR detection method and a kit for a novel coronavirus 2019-nCoV. Specifically, the present invention relates to a kit and a method for detecting the nucleic acid of an E gene of the novel coronavirus 2019-nCoV. The kit and the method have extremely high sensitivity and specificity, and can significantly improve the accuracy of virus identification.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
A kit and method for multiplex detection of 2019 novel corona virus nucleic acid, capable of simultaneously detecting two nucleic acid targets of the novel coronavirus, having extremely high sensitivity and specificity, and significantly improving the accuracy of virus identification.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Provided is a nucleic acid detection kit for novel coronavirus 2019-nCoV, and in particular, provided are a kit and a method for multiple detection of the nucleic acid of the novel coronavirus 2019-nCoV. Three nucleic acid targets of the novel coronavirus 2019-nCoV can be detected at the same time. False positives can be prevented by means of mutual authentication among different targets, and detection omissions, which may be caused by mutations, are confirmed, such that the accuracy of virus identification is significantly improved.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
DAAN GENE CO., LTD. OF SUN YAT-SEN UNIVERSITY (Chine)
Inventeur(s)
Jiang, Xiwen
Liu, Aishan
Wang, Zhouquan
Zheng, Sangsang
Zhao, Jiguang
Lian, Xianlan
Xie, Xiaocheng
Lu, Xuelan
Zhang, Wei
Xu, Haojian
Fang, Zhikeng
Wu, Zhijian
Abrégé
Provided are a thermostable reverse transcriptase mutant and the use thereof. A reverse transcriptase (M-MLV) mutation library is constructed, and a mutant with improved thermal stability and higher amplification efficiency is screened out finally through stepwise screening.
DAAN GENE CO., LTD. OF SUN YAT-SEN UNIVERSITY (Chine)
Inventeur(s)
Yang, Cui
Gao, Xiujie
Xi, Zhiyong
Zhu, Jian
Luo, Yongping
Abrégé
A fluorescence detection primer, detection method, and detection kit for Culex Wolbachia. The fluorescence detection primer is: wPipF: 5'-GTTTGTGCAGCTAATAG-3'; wPipR: 5'-GTCTGCAAGGCCTATTTCTACTG-3'; probe: 5'-CTTTCAATTGAAAAGATTCGATCAAC-3'; the two ends of the probe are respectively combined with a fluorescence generation group and a fluorescence quenching group. Culex Wolbachia can be detected by utilizing the fluorescence detection primer and the probe of the present invention on the basis of the detection method. In the method, the lowest detection limit is 100 copies/ml, and no amplification of Aedes Wolbachia occurs.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
46.
FLUORESCENCE DETECTION PRIMERS OF THREE TYPES OF WOLBACHIA AND DETECTION METHOD AND DETECTION KIT THEREOF
DAAN GENE CO., LTD. OF SUN YAT-SEN UNIVERSITY (Chine)
Inventeur(s)
Gao, Xiujie
Yang, Cui
Zhou, Qiwei
Li, Limei
Xi, Zhiyong
Zhu, Jian
Abrégé
Disclosed are fluorescence detection primers of three types of Wolbachia and a detection method and detection kit thereof. Using the fluorescence detection primers of the present invention, Aedes type A, Aedes type B and Culex Wolbachia can be detected according to the detection method of the present invention, and only Aedes Wolbachia is detected in the Aedes, while only Culex Wolbachia is detected in the Culex, and the lowest detection limit is 100 copies/ml.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
47.
FLUORESCENCE DETECTION PRIMER, DETECTION METHOD, AND DETECTION KIT FOR TYPE-A AND TYPE-B WOLBACHIA
DAAN GENE CO., LTD. OF SUN YAT-SEN UNIVERSITY (Chine)
Inventeur(s)
Yang, Cui
Gao, Xiujie
Xi, Zhiyong
Zhu, Jian
Luo, Yongping
Abrégé
A fluorescence detection primer, detection method, and detection kit for type-A and type-B Wolbachia. By using the fluorescence detection primer of the present invention, the detection method may be used to detect Aedes Wolbachia, and further to distinguish between type-A Wolbachia, type-B Wolbachia, and crosses of type-A and type-B Wolbachia. In the method, the lowest detection limit is 100 copies/ml, and no amplification of Culex Wolbachia occurs.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Diagnostic preparations for medical purposes; medicines for the treatment of gastrointestinal diseases for human purposes; bouillons, namely, bacteriological nutritive media, for bacteriological cultures; radioactive substances for medical purposes; dietetic foods adapted for medical purposes; depuratives; biocides; wadding, namely, cotton wool, for medical purposes; dental lacquer; veterinary preparations, namely, pain relief medication Computers; recorded computer software for spreadsheet, word processing; electrical heat sealing machines for sealing plastics, namely, packaging; weighing apparatus and instruments; fire extinguishers; tape measures; mechanical signs; optical apparatus and instruments, namely, optical mirrors; surveying apparatus and instruments; X-ray tubes not for medical purposes Medical x-ray apparatus and instruments; ice bags for medical purposes; gloves for medical purposes; dental apparatus, namely, X-ray appliances for dental and medical use; diagnostic apparatus for medical purposes, namely, ultrasonic medical diagnostic apparatus; apparatus and installations, namely, X-ray appliances for dental and medical use for the production of X-rays, for medical purposes; orthopedic joint implants; suture materials; furniture especially made for medical purposes, namely, dental chairs; surgical implants comprising artificial materials
