42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Data analysis services in the field of agriculture and biofuels; collection, compilation, analysis and management of data pertaining to agriculture, crops, and biofuels.
(2) Diagnostic services in the field of agriculture, crops, and biofuels; agricultural services, namely, crop data collection, crop data sampling, and crop observation for analysis purposes.
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business data analysis services in the field of agriculture and biofuels; collection, compilation, analysis and management of business data pertaining to agriculture, crops, and biofuels Diagnostic services in the nature of scientific research services in the field of agriculture, crops, and biofuels; agricultural services, namely, crop data collection, crop data sampling, and crop observation for scientific analysis purposes
3.
METHODS FOR QUANTIFYING STARCH AND CELLULOSE IN CORN SAMPLES
Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.
C12Q 1/54 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le glucose ou le galactose
G01N 1/28 - Préparation d'échantillons pour l'analyse
Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.
C12Q 1/54 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir le glucose ou le galactose
G01N 1/28 - Préparation d'échantillons pour l'analyse
6.
Cellulosic enzyme recycling from separation of saccharified biomass
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
9.
Cellulosic enzyme recycling from separation of saccharified biomass
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
C13K 1/02 - GlucoseSirops glucosés obtenus par saccharification de matières cellulosiques
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Engineering services, namely, engineering in the field of cellulosic ethanol; scientific and technological services in the field of ethanol production, namely, scientific research, analysis and consultation in the field of ethanol production; performance of chemical analyses
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Engineering services, namely, engineering in the field of cellulosic ethanol; scientific and technological services in the field of ethanol production, namely, scientific research, analysis and consultation in the field of ethanol production; performance of chemical analyses
12.
High-solids biomass slurry generation for enhanced efficiency hydrolysis processing and equipment design to yield the same
This disclosure provides a high solids biomass slurry that is readily pumpable and transportable to downstream processing units, such as chemical and/or biochemical processing units. The slurry is amenable to saccharification efficiencies of >70 % in processing times of <36 hours. Also provided are devices for processing materials, such as the high solids biomass slurry.
Described herein are beta-glucosidase enzymes that have improved beta-glucosidase activity compared to a control beta-glucosidase enzyme. The improved beta-glucosidase enzymes are useful for converting a cellulosic biomass to fermentable sugars such as glucose. Also described are isolated polynucleotides that encode polypeptides having improved beta-glucosidase activity, expression cassettes for expressing the improved beta-glucosidase polypeptides, and cells, such as yeast cells, transformed with the expression cassettes.
C12P 19/14 - Préparation de composés contenant des radicaux saccharide préparés par action d'une carbohydrase, p. ex. par action de l'alpha-amylase
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
14.
Cellulosic enzyme recycling from separation of saccharified biomass
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
C13K 1/02 - GlucoseSirops glucosés obtenus par saccharification de matières cellulosiques
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
C13K 13/00 - Sucres non prévus ailleurs dans la présente classe
15.
Advanced auger and filtration system for the saccharification of biomass
The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C13K 1/02 - GlucoseSirops glucosés obtenus par saccharification de matières cellulosiques
C07H 1/08 - SéparationPurification à partir de produits naturels
C12P 19/14 - Préparation de composés contenant des radicaux saccharide préparés par action d'une carbohydrase, p. ex. par action de l'alpha-amylase
16.
Recovery of desired co-products from fermentation stillage streams
Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
C11B 1/10 - Production des graisses ou huiles à partir de matières premières par extraction
C11B 13/00 - Obtention de graisses, huiles ou acides gras à partir de résidus
17.
Acetate resistance in yeast based on introduction of a mutant HAA1 allele
Improved haa1 transcriptional regulatory proteins, polynucleotides encoding improved haa1 transcriptional regulatory proteins and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved haa1 transcriptional regulatory proteins and cells expressing heterologous haa1 transcriptional regulatory proteins as disclosed herein.
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
C12P 7/10 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique préparé comme sous-produit, ou préparé à partir d'un substrat constitué par des déchets ou par des matières cellulosiques d'un substrat constitué par des matières cellulosiques
C07K 14/395 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons provenant de levures provenant de Saccharomyces
C12N 1/22 - Procédés utilisant de la cellulose ou ses hydrolysats ou milieux de culture en contenant
18.
Recovery of desired co-products from fermentation stillage streams
Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.
Improved manganese peroxidases, polynucleotides encoding improved manganese peroxidase and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved manganese peroxidases and cells expressing a heterologous manganese peroxidase as disclosed herein.
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
C12P 7/14 - Fermentation en plusieurs étapesFermentation avec différents types de micro-organismes ou avec réemploi de micro-organismes
C12N 9/08 - Oxydoréductases (1.), p. ex. luciférase agissant sur le peroxyde d'hydrogène comme accepteur (1.11)
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
Produits et services
[ Fermentation of corn to produce ethanol for others; production of bio-fuels and alternative fuels for others; ] consulting in the field of bio-fuel and alternative fuel production
22.
Recovery of desired co-products from fermentation stillage streams
Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.
The present invention provides for combinations of enzymes and other proteins that result in improved saccharification of plant material. The invention provides for saccharification in the presence of and optional fermentation by, yeast cells expressing the enzymes and other proteins.
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 9/24 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2)
C12N 9/42 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons bêta-glucosidiques-1, 4, p. ex. cellulase
C12N 9/44 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons alpha-glucosidiques-1, 6, p. ex. iso-amylase, pullulanase
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
24.
Materials and methods for converting biomass to biofuel
This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
Produits et services
(1) Fermentation of corn to produce ethanol for others; development and production of bio-fuels and alternative fuels; consulting in the field of bio-fuels and alternative fuels.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
Produits et services
(1) Fermentation of corn to produce ethanol for others; development and production of bio-fuels and alternative fuels; consulting in the field of bio-fuels and alternative fuels.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
Produits et services
[ Fermentation of corn to produce ethanol for others; production of bio-fuels and alternative fuels for others; ] consulting in the field of bio-fuel and alternative fuel production