Provided are a gene sequencing data compression method and apparatus, and a gene sequencing data decompression method and apparatus. The gene sequencing data compression method comprises: on the basis of a sequence prediction model obtained by jointly training a sample base sequence and a corresponding sample quality value sequence, performing sequence prediction on at least one of a base sequence and a quality value sequence in gene sequencing data to be compressed, and performing entropy encoding on at least one of the base sequence and the quality value sequence on the basis of a sequence prediction result and a corresponding ground truth label to obtain compressed data of said gene sequencing data. The gene sequencing data decompression method comprises: on the basis of a sequence prediction model obtained by jointly training a sample base sequence and a corresponding sample quality value sequence, performing sequence prediction on gene sequencing data to be decompressed, and on the basis of a prediction result, obtaining decompressed data corresponding to said gene sequencing data.
The present invention relates to the technical field of biology, and in particular to a polynucleotide kinase mutant and a use thereof. The polynucleotide kinase mutant comprises, compared with a wild type polynucleotide kinase, at least one amino acid mutation in the following sites or functional equivalent sites: the 47th site and the 129th site; and the wild type polynucleotide kinase has an amino acid sequence as shown in SEQ ID NO: 1.
The present application relates to the field of biomedicine, and provides a base recognition method, a base recognition model training method, and an electronic device. The base recognition method comprises: inputting sequencing data into a pre-trained base recognition model, using a feature encoder of the base recognition model to acquire multiple encoded feature vectors of the sequencing data, and using a context network of the base recognition model to extract temporal information and contextual relationships of the multiple encoded feature vectors, thereby obtaining multiple contextual feature vectors having contextual association features, and then using a decoder to decode the contextual feature vectors, which can improve the accuracy of recognizing a base sequence from sequencing data. The base recognition model training method achieves algorithm development of the base recognition model by means of a pre-training + fine-tuning training approach. The use of the aforementioned method can reduce the training costs of the base recognition model and improve the accuracy of prediction of the model, thereby enhancing the accuracy of base recognition.
A liquid injection device, a liquid injection method, and a micro-fluidic system. The liquid injection device (100) has a liquid injection mode and a liquid supplement mode; in the liquid injection mode, a transmission liquid is injected into a first cavity (111), and then the transmission liquid acts on a first surface (311) and drives, by means of a first connecting part (33), a second sliding part (32) to slide, so that a reaction liquid in a fourth cavity (212) or the downstream section of the fourth cavity (212) is injected into a reaction device (200); and in the liquid supplementing mode, the transmission liquid is injected into a second cavity (112), and then the transmission liquid acts on a second surface (312) and drives, by means of the first connecting part (33), the second sliding part (32) to slide, so that the fourth cavity (212) or the downstream section of the fourth cavity (212) accommodates the injected reaction liquid. The area of the transmission liquid acting on the first surface (311) is greater than the area of the transmission liquid acting on the second surface (312), so that slow liquid injection and rapid liquid supplementation can be realized, and the slow liquid injection is beneficial to accurately and slightly controlling the liquid injection amount.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
5.
NUCLEIC ACID MOLECULE SEQUENCING METHOD AND RELATED DEVICE
A nucleic acid molecule sequencing method of the present disclosure, comprising: first acquiring a sequencing image of a target nucleic acid sample; performing image processing on the sequencing image by means of an image processing model, so as to obtain a target image, the image processing model being a model obtained by training constructed nucleic acid molecule true value images and corresponding simulation nucleic acid images; and performing nucleic acid molecule sequencing on the basis of the target image, so as to obtain a sequencing result corresponding to the target nucleic acid sample.
Provided are a DNA polymerase, and a preparation method therefor and the use thereof. The DNA polymerase has less than 42% sequence similarity to Taq DNA polymerase, and has polymerase activity, higher thermal stability, 5'-3' exonuclease activity and inhibition resistance.
Disclosed are a terminal deoxyribonucleoside transferase mutant, a preparation method therefor and a use thereof. A wild-type terminal deoxyribonucleoside transferase is subjected to molecular modification, to improve modified nucleic acid monomer polymerization activity and modified nucleic acid monomer catalysis efficiency, and to realize efficient single-base addition efficiency. Multiple 3'-O-blocking modified dNTPs substrates can be added to a 3'-OH end of an oligonucleotide single chain without a template, so that a novel and effective tool enzyme can be provided for the enzymatic method from de novo synthesis of nucleic acid.
The present application provides an isolated polypeptide, a preparation method therefor, and use thereof. The polypeptide has an amino acid sequence that is at least 99% homologous with SEQ ID NO:1, and that is preferably 100% homologous therewith.
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
9.
METHOD FOR DETERMINING CONSENSUS SEQUENCE OF NUCLEIC ACID MOLECULES AND USE
Disclosed in the present application are a method for determining a consensus sequence of nucleic acid molecules and the use. The method comprises the following steps: acquiring sequencing information of the nucleic acid molecules, wherein the sequencing information comprises multi-copy sequences of library molecules, and the library molecules comprise single-stranded circular molecules formed by ligation of the nucleic acid molecules and feature fragments; identifying feature sequences of the feature fragments in the sequencing information on the basis of a Levenshtein distance algorithm and by means of known sequences of the feature fragments; dividing the sequencing information according to the feature sequences, and determining repetitive nucleic acid sequences of the nucleic acid molecules; and generating the consensus sequence according to a set of the repetitive nucleic acid sequences of the nucleic acid molecules.
Embodiments of the present application belong to the field of genetic variation detection, and provide a genetic variation detection method and apparatus, a storage medium, and a computer device. The method comprises: by means of a high-quality single-base variation site measured by a model for performing genetic variation detection on the basis of gene accumulation, determining, from a reference haplotype set, a haplotype of a gene segment under test, and, on the basis of the haplotype, determining an insertion/deletion site of the gene segment under test.
The present application provides a nucleic acid array for detecting spatial information of nucleic acids in a sample, a method for detecting spatial information of nucleic acids in a sample on the basis of the array, and a method for producing the nucleic acid array.
Provided are a protein sequencing library construction method and a protein sequencing method. The protein sequencing library construction method comprises: using an endonuclease to carry out enzymatic cleavage on a target protein to obtain two or more polypeptide fragments each containing a free amino group at the N-terminus and the same functional group at the C-terminus, the functional group comprising an amino, ε-carboxyl or phenolic hydroxyl group; using the functional group at the C-terminus and the free amino group at the N-terminus to conjugate each polypeptide fragment to a nucleic acid to obtain a protein sequencing library, wherein the nucleic acid is conjugated to the functional group at the C-terminus and the free amino group at the N-terminus of the polypeptide fragment, respectively. The present invention can solve the problem in the prior art of the difficulty in sequencing of native proteins (i.e., proteins with unknown sequences), and is applicable to the field of protein sequencing.
Provided in the present application are an isolated polypeptide, a preparation method, and a use. The polypeptide has an amino acid sequence which is at least 91% homologous or 92% homologous or 93% homologous or 94% homologous or 95% homologous or 96% homologous or 97% homologous or 98% homologous or 99% homologous to SEQ ID NO: 1.
Provided are a single-cell chromatin accessibility and transcriptome joint sequencing method and a use thereof. The method comprises: using a transposase to process a nucleus so as to obtain gDNA of a chromatin open region linked to a first specific tag sequence; using a transcriptome capture sequence to process the nucleus so as to obtain cDNA linked to a second specific tag sequence; generating a droplet on the basis of droplet microfluidics to encapsulate the nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and one or more nuclei are encapsulated in the droplet; and sequencing the gDNA and the cDNA, and obtaining single-cell chromatin accessibility and transcriptome joint information on the basis of the first specific tag sequence and the second specific tag sequence.
The present invention provides a male infertility classification method and device and a method and device for constructing a related prediction model. The classification method comprises: using a contribution proportion of testis-related single-cell types in seminal plasma cell-free RNA as a feature to construct a male infertility prediction model; and inputting the contribution proportion of the testis-related single-cell types in the seminal plasma cell-free RNA of a sample to be tested into the male infertility prediction model to obtain a classification prediction result of the sample to be tested. The classification method can accurately predict male azoospermia and subtypes thereof of an unknown sample, and is more convenient to operate compared with a previous method. In addition, the method allows for prediction of the subtypes of azoospermia prior to clinical testicular biopsy, thereby achieving the objectives of non-invasive detection and diagnosis.
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
Provided are a T4 DNA ligase mutant and the use thereof. The T4 DNA ligase mutant: a) has an amino acid sequence having at least 80% identity to SEQ ID NO: 1; b) comprises a substitution of an amino acid at the position corresponding to position 28 of SEQ ID NO: 1; and c) has a T4 DNA ligase activity. The T4 DNA ligase mutant has improved double-end linker connection efficiency and single-chain cyclization performance, and is compatible with different reaction systems. The T4 DNA ligase mutant can be widely used in library construction for various high-throughput sequencing, single-chain cyclization and ligation of DNA fragments in molecular cloning.
Provided are a Ski2-like helicase and a use thereof. The Ski2-like helicase comprises: 1) a protein having an amino acid sequence as shown in any one of SEQ ID NO: 1 to 4; or 2) a protein in which at least one site in the 2A domain and/or the Ratchet domain of the amino acid sequence in 1) is replaced by cysteine or an unnatural amino acid, and/or at least one cysteine in any one or more domains of the 2A domain, WH domain, and Ratchet domain is deleted or replaced by a natural amino acid other than cysteine, and which has helicase activity for unwinding nucleic acids in the 3' to 5' direction. The described helicase can form stable channels, has the function of unwinding nucleic acids in the 3' to 5' direction, and can broaden the application scenarios for nanopore sequencing.
The present invention provides a polypeptide and protein sequencing method, a kit, and a use. The polypeptide sequencing method comprises: S1, attaching a target polypeptide to a nanopore; and S2, introducing a recognition molecule, which specifically recognizes a terminal amino acid of the target polypeptide, into a nanopore sequencing system, and under the action of an electric field, using an electric signal change generated during binding/disassociation of the recognition molecule and the target polypeptide to detect the terminal amino acid of the target polypeptide. The detection of a single amino acid is achieved by using a unique electric signal change generated during binding/dissociation of the recognition molecule and the recognized terminal amino acid of the target polypeptide.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
19.
METHOD FOR IMPROVING H&E-STAINED IMAGE QUALITY OF SPATIO-TEMPORAL CHIP, REAGENT COMBINATION, KIT, AND DEVICE
The present invention relates to the technical field of biology, and in particular to a method for improving the H&E-stained image quality of a spatio-temporal chip, a reagent combination, a kit, and a device. The present invention provides a new mounting medium, which uses a simple reagent and corresponding process design, achieving the same effects of mRNA in-situ capture and comprehensive enhancement of mRNA diffusion as a glycerol mounting medium, and effectively solving the problems of fading and color diffusion after tissue staining. Registration of an H&E image and a spatio-temporal expression matrix of a BGI Stereo-seq chip provides possibility for high-precision subcellular-level omics analysis.
Provided are a nucleic acid-polypeptide-nucleic acid ternary complex, and a use thereof in polypeptide nanopore sequencing. The ternary complex has the following structural formula: single-stranded nucleic acid 1-polypeptide-single-stranded nucleic acid 2, the single-stranded nucleic acid 1, the polypeptide and the single-stranded nucleic acid 2 being covalently linked in sequence, the single-stranded nucleic acid 1 being used to connect with a sequencing adapter, the single-stranded nucleic acid 1 and/or the single-stranded nucleic acid 2 each independently further comprising an extension chain, the extension chain being disposed at either end or in the middle of the single-stranded nucleic acid 1 and/or the single-stranded nucleic acid 2, the extension chain comprising a chain formed from one or more of the following molecules in random order: nucleosides, nucleotides or organic linkers, and the extension chain not being a sequence of multiple nucleotides when it is located on the single-stranded nucleic acid 2. Extending the length of the single-stranded nucleic acid at at least one end of the polypeptide is beneficial for reducing the phenomenon of stutter generated during a polypeptide nanopore sequencing process.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 27/00 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques
G01N 33/483 - Analyse physique de matériau biologique
C40B 40/10 - Bibliothèques comprenant des peptides ou des polypeptides ou leurs dérivés
C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C07K 7/06 - Peptides linéaires ne contenant que des liaisons peptidiques normales ayant de 5 à 11 amino-acides
21.
IMAGING SYSTEM AND METHOD, IDENTIFICATION APPARATUS AND METHOD, SEQUENCER, AND SEQUENCING METHOD
An imaging system and method, an identification apparatus and method, a sequencer, and a sequencing method. The imaging system comprises: a laser light source and a structured light modulation element, the structured light modulation element being used for modulating an excitation light, so as to generate and emit a first light and a second light having separate optical paths; a light guide element, configured to, in a first angle state, guide the first light and the second light such that interference occurs on the surface of a test sample to form a fringe structured light, and in a second angle state, guide the first light or the second light to form a wide-field light on the surface of the test sample, wherein the fringe structured light excites and generates a structured light fluorescence signal, and when the light guide element emits the first light or the second light at the second angle, the first light or the second light forms a wide-field light spot on the surface of the test sample, so as to excite and generate a wide-field fluorescence signal; and an imaging module, used for acquiring the structured light fluorescence signal to generate a structured light image, and acquiring the wide-field fluorescence signal to generate a wide-field image.
The present invention provides a DNA polymerase mutant and a use thereof. The DNA polymerase mutant has DNA polymerization activity, and comprises an amino acid sequence having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% homology with the amino acid sequence shown in SEQ ID NO: 1. The invention can solve the problem of low polymerization activity of wild-type DNA polymerases in the prior art, and is applicable in the field of DNA polymerases.
Provided in the present invention are a DNA polymerase and the use thereof. The above-mentioned DNA polymerase comprises: (a) a protein having a sequence as shown in SEQ ID NO: 1; (b) a protein having substitution, deletion, and/or addition of one or more amino acids at at least one of the following sites in the sequence as shown in SEQ ID NO: 1: D147, E149, L424, Y425, P426 and A503; or (c) a protein having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% or more homology to the sequence in (a) or (b), and having a DNA polymerase activity. The present invention can solve the problem of the limited types of DNA polymerases in the prior art, and is suitable for the field of DNA polymerases.
Provided are a transcriptome library construction method based on multiple displacement amplification, a kit used for said method, and a transcriptome sequencing method and a spatial transcriptome sequencing method based on this method. The method comprises: reverse-transcribing an mRNA molecule to obtain a cDNA chain; using the cDNA chain as a template, performing multiple displacement amplification under the actions of MDA primers and a DNA polymerase having chain displacement activity, to obtain multiple cDNA amplified fragments of different lengths, the cDNA amplified fragments constituting a transcriptome library, there being multiple MDA primers, and the MDA primers containing a first sequence used for sequencing and a second sequence used to bind to the cDNA chain, the second sequence being a random sequence, the second sequence randomly binding to the cDNA chain, and, optionally, the first sequence being a first linker, and the second sequence also containing degenerate bases.
The present application provides a multi-modal feature fusion method, a model training method, an electronic device, and a storage medium. The multi-modal feature fusion method comprises: inputting the obtained transcriptome information and imaging information into a preset multi-modal fusion model to obtain a multi-modal fusion feature, comprising: inputting the transcriptome information into a first single-modal feature extractor to obtain a transcriptome feature, and inputting the imaging information into a second single-modal feature extractor to obtain an imaging feature; performing attention mechanism-based information fusion on the transcriptome feature and the imaging feature, and concatenating the features into a feature vector; and encoding the feature vector and a spatial position map into a first latent variable on the basis of an auto-encoder of a graph generation model. The present application can improve the fusion performance of multi-modal features.
G06V 10/80 - Fusion, c.-à-d. combinaison des données de diverses sources au niveau du capteur, du prétraitement, de l’extraction des caractéristiques ou de la classification
Provided are a method for constructing an RNA array, a kit for preparing an RNA array, a method and device for detecting RNA-protein interaction, and a kit. The method comprises: on the basis of a first nucleic acid fixed on a first chip, transcribing the first nucleic acid to obtain a target RNA chain; and on the basis of a second nucleic acid fixed on a second chip, capturing the target RNA chain by using the second nucleic acid, so as to obtain the RNA array.
Provided in the present invention are the construction and application of a polypeptide classifier. Specifically, provided in the present invention is a polypeptide classification method. The method comprises: a sequencing step, which comprises: sequencing a polypeptide to be tested or a linker containing said polypeptide, acquiring a sequencing signal of said polypeptide, and obtaining a spectrum signal of said polypeptide from the sequencing signal; a feature extraction step, which comprises: by means of a feature set function, respectively extracting a plurality of feature values from the sequencing signal and the spectrum signal, so as to form a feature set; and a prediction step, which comprises: inputting the feature set into a polypeptide classification model for classification, wherein a category with the highest frequency of occurrence among output categories is the category of said polypeptide. By means of the method of the present invention, nano-sequencing signals of more types of polypeptides can be more accurately classified, and the accuracy rate can reach 95.52%.
Provided in the present disclosure is an object detection model-based polypeptide signal extraction method. Provided in the present disclosure is a polypeptide signal extraction method, which comprises: obtaining from a sequencing sample an image containing a sequencing signal, the sequencing sample comprising a molecule under test having a polypeptide portion and a non-polypeptide portion (NPP), and the sequencing signal comprising a mixed sequencing signal having a polypeptide signal and a non-polypeptide signal (NPS); using a neural network model to identify the coordinates of a bounding box of the non-polypeptide signal in the image, and converting the coordinates into time index points in an original sequencing signal; and, by means of the time index points, extracting the polypeptide signal from the mixed sequencing signal.
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
30.
FLUID PATH AND USE METHOD THEREFOR, FLUID PATH SYSTEM AND USE METHOD THEREFOR, AND SEQUENCER
Provided are a fluid path and a use method therefor, a fluid path system and a use method therefor, and a sequencer. The fluid path comprises a first surface and a second surface; the first surface at least comprises an exposed chip surface; the first surface and the second surface are arranged at a preset distance; a preset position of the second surface is provided with a reagent fluid; and the second surface can move in a preset direction, and drive the reagent fluid at the preset position to be at least injected into a gap between the chip surface and the second surface. Movement of the second surface drives the reagent fluid to move, thus avoiding using pressure to drive the reagent fluid; the chip surface is in a static state, such that the average flow rate of the fluid path is half of the movement speed of the second surface, and the thickness of the fluid entering the gap will be at least twice the original thickness, thereby reducing the reagent thickness of fluid intake; and the fluid path is an open fluid path, and the first surface and the second surface do not need to be fixed by adhesive bonding, thus overcoming the technical challenge of sealing fluid paths under the condition of small gaps.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B05C 9/00 - Appareillages ou installations pour appliquer des liquides ou d'autres matériaux fluides aux surfaces par des moyens non prévus dans l'un des groupes , ou dans lesquels le moyen pour déposer le liquide ou autre matériau fluide n'est pas important
31.
METHOD FOR MODIFYING SOLID-PHASE SUPPORT AND METHOD FOR CELL CAPTURE
Provided in the present invention is a method for modifying a solid-phase support. The solid-phase support is suitable for adhering to cells. The method comprises: bringing a solid-phase support into contact with a polylysine–protein factor mixed solution, wherein the polylysine–protein factor mixed solution comprises polylysine and a protein factor, and the protein factor comprises at least one of an extracellular matrix protein and a cellular integrin. In the method, by modifying the solid-phase support, a large number of cells can be captured by the solid-phase support, and the cells captured by the solid-phase support have clear outlines, such that the cell capture efficiency of the solid-phase support is improved.
The present application relates to a feature extraction method and apparatus for cell grouping, and a cell grouping method and apparatus. The feature extraction method for cell grouping comprises: performing disturbance on a cell feature matrix of a sample cell, so as to obtain a first feature matrix and a second feature matrix; performing disturbance on an adjacency matrix of the sample cell, so as to obtain a first adjacency matrix and a second adjacency matrix; by means of a feature encoder to be trained, performing encoding on a first image to obtain a first embedding matrix, and performing encoding on a second image to obtain a second embedding matrix; fusing the first embedding matrix with the second embedding matrix, so as to obtain a target embedding matrix; performing decoding on the target embedding matrix, so as to obtain at least one of a reconstructed cell feature matrix and a reconstructed adjacency matrix; and on the basis of at least one of a first difference and a second difference, iteratively optimizing parameters of said feature encoder until an iteration stop condition is met, so as to obtain a trained feature encoder, wherein the feature encoder is used for extracting features required for cell grouping.
G06V 10/44 - Extraction de caractéristiques locales par analyse des parties du motif, p. ex. par détection d’arêtes, de contours, de boucles, d’angles, de barres ou d’intersectionsAnalyse de connectivité, p. ex. de composantes connectées
33.
SINGLE MOLECULE DETECTION SYSTEM AND SINGLE MOLECULE DETECTION METHOD
A single molecule detection system and a single molecule detection method. The single molecule detection system comprises: a surface plasmon resonance (SPR) sensor (100), comprising a first surface (111) and a second surface (112), wherein the first surface (111) is configured to bind to a first molecule (610), and the first molecule (610) is configured to bind to a second molecule (620) marked with a fluorescent substance (621); a light source (200), configured to provide incident light to the second surface (112) to excite surface plasmon polaritons (SPP), so as to generate SPR; a first detection portion (300), configured to detect optical parameters of the SPR; and a second detection portion (400), configured to detect a fluorescence signal generated by the second molecule (620). On the basis of the resonance and fluctuation characteristics of the SPPs, a marked signal feature and an unmarked signal feature are synchronously obtained on two sides of a surface of the SPR sensor (100), so that qualitative or quantitative measurement of features such as loading amount, positioning, affinity to a substrate, and a molecular docking rate of the unmarked first molecule (610) is realized, and dynamic interaction processes between molecules such as binding and dissociation are synchronously measured.
G01N 33/533 - Production de composés immunochimiques marqués avec un marqueur fluorescent
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
Provided is a new reverse transcriptase or a biologically active fragment thereof. The reverse transcriptase or biologically active fragment thereof comprises a mutated sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% identity to SEQ ID NO: 1, wherein the mutation comprises at least one of substitution, deletion and insertion.
Disclosed in the present invention is a method for constructing a sequencing library. The method comprises: (1) performing reverse transcription treatment on a mRNA to obtain a reverse transcription product without the need of adding a template switch oligo, the 3' end of the mRNA containing a poly-A sequence, the mRNA being connected to a chip, the chip being connected to a probe containing a poly-T sequence, and the connection being realized by means of the complementary pairing of the Poly-A sequence at the 3' end of the mRNA and the poly-T sequence on the chip; and (2) performing fragmentation on the reverse transcription product and ligating a first adapter to same so as to obtain a fragmentated and adapter-ligated product, the fragmentated and adapter-ligated product being connected to the chip; and (3) releasing the fragmentated and adapter-ligated product from the chip, so as to obtain a sequencing library.
A novel Cas12a nuclease BEST5 and a use thereof in gene editing and nucleic acid detection. The novel Cas12a protein, namely, BEST5, can be used in a nucleic acid detection system and can be used as a genome editing tool enzyme, providing more tools and effective site selection for in-vivo gene editing application.
Disclosed is a use of Lactobacillus gasseri TF08-1 in the prevention and treatment of Helicobacter pylori infection. The strain has the beneficial effect of inhibiting the proliferation of Helicobacter pylori and urease activity in vitro; in animal experiments, probiotics (live bacteria preparations) and postbiotics (inactivated bacteria preparations) thereof were able to effectively prevent and treat Helicobacter pylori infection, inhibit the proliferation of Helicobacter pylori in the body, and improve gastric mucosal damage and systemic inflammatory response caused by Helicobacter pylori infection.
A61K 35/747 - Lactobacilles, p. ex. L. acidophilus ou L. brevis
A61P 1/04 - Médicaments pour le traitement des troubles du tractus alimentaire ou de l'appareil digestif des ulcères, des gastrites ou des œsophagites par reflux, p. ex. antiacides, antisécrétoires, protecteurs de la muqueuse
A packaging device, a preparation method therefor and an electronic apparatus. The packaging device comprises a circuit board, a heat conduction block and an electronic element. The circuit board comprises a first surface and a second surface which are oppositely arranged in a first direction, and the circuit board is provided with a through slot passing through the first surface and the second surface. The heat conduction block is fixed on the circuit board and is at least partially located in the through slot. The heat conduction block comprises a third surface and a fourth surface which are oppositely arranged in the first direction, and the third surface is exposed out of the opening of the through slot at the first surface. The electronic element is fixed on the third surface. The electronic element comprises a central region and an edge region arranged around the central region. Viewed from the first direction, the third surface overlaps with the central region. The present application can improve the heat dissipation efficiency and the temperature uniformity of the surface of the electronic element.
A method for embedding a nanopore protein into a biomimetic membrane and the use thereof. The method comprises: preparing a biomimetic membrane of a multi-block copolymer, wherein the multi-block copolymer comprises a hydrophilic segment and a hydrophobic segment; and embedding a nanopore protein into the biomimetic membrane, wherein the multi-block copolymer comprises a copolymer of two or more blocks, the biomimetic membrane comprises an aromatic ring structure, the aromatic ring structure is linked between the hydrophilic segment and the hydrophobic segment of the multi-block copolymer, and/or the nanopore protein comprises an aromatic ring structure, and the aromatic ring structure is located on an amino acid in the transmembrane region of the nanopore protein facing the biomimetic membrane. By means of the method, the number of single pores and/or the single-pore retention rate of a biomimetic membrane during embedding can be improved.
B01D 71/82 - Matériaux macromoléculaires non prévus spécifiquement dans un seul des groupes caractérisés par la présence de groupes déterminés, p. ex. introduits par un post-traitement chimique
40.
GENE EXPRESSION MATRIX OPTIMIZATION METHOD, ELECTRONIC DEVICE, AND STORAGE MEDIUM
Provided are a gene expression matrix optimization method, an electronic device, and a storage medium. The method comprises: acquiring cell data of a plurality of cells, wherein the cell data comprises an initial gene expression matrix of each of the plurality of cells; determining a preset number of nearest neighbor cells of a target cell among the plurality of cells on the basis of the cell data; determining a gene expression distance between the target cell and each nearest neighbor cell, determining a distance parameter on the basis of a target non-zero value among the gene expression distances, and determining, on the basis of the distance parameter, a weight parameter corresponding to each nearest neighbor cell; and on the basis of the weight parameter corresponding to each nearest neighbor cell and the initial gene expression matrix of each nearest neighbor cell, obtaining a target gene expression matrix obtained by smoothing of the target cell. By using the described method, the noise in a gene expression matrix can be reduced to improve the accuracy of performing downstream analysis on the basis of the gene expression matrix.
A biochip, a preparation method therefor and the use thereof. The biochip is a double-sided porous nano-biochip, which has an anodized nanostructure on the surface thereof; and the surface of the chip can be chemically modified to provide, for RNA synthesis, linking molecules required for a synthesis reaction, thus enabling the attachment of monomers to the surface of the biochip. The biochip can be prepared by using an anodization method which is easily operated and is low cost, thereby lowering the difficulty of the preparation process, and providing synthetic products having a higher loading capacity at a lower price.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
42.
METHOD AND APPARATUS FOR TISSUE TRACKING CELL FREE RNA
Provided are a method and apparatus for performing tissue tracking of cell free RNA, a device, a readable storage medium, a computer program product, and a computer program. The method for performing tissue tracking of cell free RNA comprises: using one or more transcriptome data sets to construct a tissue characteristic gene matrix; on the basis of a cell free RNA expression profile of a sample and the tissue characteristic gene matrix, calculating a tissue contribution score; and, on the basis of the tissue contribution score, performing tissue tracking of the cell free RNA.
A structured-light illumination system, a structured-light illumination method, and a super-resolution optical system. The structured-light illumination system comprises: at least two light sources (1), wherein the light sources (1) are used to generate light beams of a preset wavelength; at least two gratings (2) respectively located on the optical paths of the at least two light sources (1), wherein the gratings (2) are used to generate interference fringes; displacement platforms (3), wherein the gratings (2) are disposed on the displacement platforms (3), and the displacement platforms (3) are used to move the gratings (2) so as to perform phase switching; and a light beam converging and guiding unit (4), wherein the optical paths intersect at the light beam converging and guiding unit (4), and the light beam converging and guiding unit (4) is used to guide the light beams to converge to an object space. The structured-light illumination system can perform rapid angle and phase switching on structured-light fringes and increases imaging speed, thus increasing the throughput of the optical system, and greatly reducing imaging costs while improving the resolution of the optical system.
An automatic liquid replenishing device and method for a sequencer. In the automatic liquid replenishing device, a first connector is arranged above a sequencer and is communicated with a liquid inlet of a sequencing chip; a second connector is communicated with a first liquid storage device; a third connector is communicated with a second liquid storage device; a fourth connector is communicated with a third liquid storage device; two ends of a first connecting pipe are respectively communicated with the first connector and the second connector; two ends of a second connecting pipe are respectively communicated with the first connector and the third connector; two ends of a third connecting pipe are respectively communicated with the third connector and the fourth connector; a fourth connecting pipe is communicated with the fourth connector; a first peristaltic pump is arranged on the path of the first connecting pipe; and a second peristaltic pump is arranged on the path of the fourth connecting pipe.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 1/14 - Dispositifs d'aspiration, p. ex. pompesDispositifs d'éjection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
45.
EMBRYO-LIKE MODEL, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES (Chine)
Inventeur(s)
Esteban, Miguel
Lai, Yiwei
Mazid, Md·abdul
Li, Jinxiu
Jia, Wenqi
Fu, Lixin
Zuo, Jing
Li, Wenjuan
Wu, Liang
Abrégé
Provided is an embryo-like model construction method. The method comprises: culturing primordial stem cells in vitro or injecting primordial stem cells into an immunodeficient animal for differentiation, so as to obtain an embryo-like model. By means of the method, during the preparation of the embryo-like model, there is no need to add cytokines and small molecule pathway interference factors, and there is no need to perform gene editing and only a single initiating cell type is used. The method has the advantages of simplicity and high repeatability, and is beneficial to large-scale preparation and application. Moreover, the prepared embryo-like model has all extraembryonic cell lineages and all embryonic cell lineages other than trophectoderm, can simulate the development process of a post-implantation embryo of Carnegie stage 3 to Carnegie stage 9 neurula, and then can be used for preparation of a variety of progenitor cell lineages, disease modeling, drug screening, and scientific researches.
Provided in the present invention are a porin-membrane fusion method, a porin insertion buffer solution and the use. Said fusion method comprises: providing a first solution at a first side of a membrane and providing a second solution at a second side of the membrane; applying a voltage to both sides of the membrane to perform porin-membrane fusion; measuring a current in the solution; and when the current increases which indicates a porin has been inserted into the membrane, then adjusting the voltage to 0 V, thereby completing the porin-membrane fusion. The first solution comprises a first buffer solution containing the porin, and the second solution comprises a second buffer solution which does not contain the porin, the osmotic pressure of the first solution being greater than the osmotic pressure of the second solution. The present invention can resolve the issue of the single-pore ratio during porin-membrane fusion in the prior art, and is suitable for the field of biological analysis and detection.
G01N 27/26 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables électrochimiquesRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en utilisant l'électrolyse ou l'électrophorèse
Disclosed in the present invention is an immune repertoire sequencing method, comprising the following steps: 1) constructing a cDNA library of a sample, cDNA in the cDNA library being linked with position information of the cDNA on the sample; 2) carrying out targeted enrichment of cDNA of a TCR gene and/or a BCR gene in the cDNA library to obtain an immune repertoire of the sample; and 3) sequencing the immune repertoire. According to the method of the present invention, targeted enrichment and high-throughput sequencing of a TCR sequence and/or a BCR sequence are innovatively achieved, and at the same time, gene expression information and spatial position information of each cell in the space are detected.
A microscopic imaging apparatus, a sequencing device, and a microscopic imaging method. The microscopic imaging apparatus comprises a light source (10), an illumination light path assembly, an imaging light path assembly, and an imaging processing apparatus (14); the illumination light path assembly and the imaging light path assembly are arranged opposite to each other, and an illumination chip (12) is arranged between the illumination light path assembly and the imaging light path assembly; the light source (10) irradiates, by means of the illumination light path assembly, light having different irradiation angles to the illumination chip (12), so that the illumination chip (12) generates plasma structured light, wherein a substrate of the illumination chip (12) is made of a transparent material; and the imaging processing apparatus (14) captures, by means of the imaging light path assembly, an original image of a fluorescence signal of the illumination chip (12) under the irradiation of light having different irradiation angles.
Disclosed in the present invention are a liquid transfer device, a reaction device and an analysis device, the liquid transfer device being used for guiding a reaction liquid to a reaction surface of a sample carrier. A fixing portion comprises a fixing structure, the fixing structure being used for fixing a sample carrier. A first substrate comprises a first wall surface, the first substrate having a first position and a second position which are relative to the fixing portion. When the first substrate is located at the first position, the first wall surface is used for obtaining a reaction liquid, and when the first substrate is located at the second position, the first wall surface is used for guiding the reaction liquid to a reaction surface. A first driving portion is configured to drive the first substrate to perform position switching between the first position and the second position. The Mohs hardness of the first substrate is greater than or equal to 5H. The present invention can effectively control the distance between the first wall surface and the reaction surface of the sample carrier, thereby ensuring the quality of reaction liquid transfer, and avoiding damage to the reaction surface caused when the first wall surface is in contact with the reaction surface, or failure to completely transfer the reaction liquid to the reaction surface caused by an excessively large distance between the first wall surface and the reaction surface.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
50.
LIQUID TRANSFER DEVICE, REACTION DEVICE, ANALYSIS DEVICE, AND LIQUID TRANSFER METHOD
Disclosed in the present invention are a liquid transfer device, a reaction device, an analysis device, and a liquid transfer method. The liquid transfer device is used for transferring a liquid carried by a first substrate to a sample carrier. The liquid transfer device comprises a fixing part and a first supporting part. The fixing part comprises a fixing structure, and the fixing structure is used for fixing the sample carrier. The first supporting part and the fixing structure are spaced oppositely in a first direction. The first supporting part has a first supporting surface. The first supporting surface is configured to at least partially face a reaction surface of the sample carrier after the sample carrier is fixed by the fixing structure. The first supporting surface is used for supporting the first substrate, so that the liquid carried by the first substrate supported by the first supporting surface is transferred to the reaction surface of the sample carrier fixed by the fixing structure. According to the liquid transfer device, the reaction device, the analysis device, and the liquid transfer method of the present application, shaking of the first substrate can be effectively inhibited, thereby preventing the first substrate from scraping the sample carrier and improving the stability of liquid transfer.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
51.
ELECTROCHEMICAL BUFFER SOLUTION FOR BIOLOGICAL NANOPORES AND PREPARATION METHOD THEREFOR
The present invention relates to the field of biochemical detection, and specifically relates to an electrochemical buffer solution for biological nanopores and a preparation method therefor. Provided is an electrochemical buffer solution which has high cost performance and is capable of reducing the loss of biological nanopores. The buffer solution comprises potassium ferricyanide, potassium ferrocyanide, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, EDTA and mannitol. When the electrochemical buffer solution is used for biological nanometer detection, the loss rate of biological nanopores can be significantly reduced, the stability of biological nanopores is improved, the flux of detection is increased, and the problem of biological nanopore loss is effectively overcome. Moreover, the electrochemical buffer solution can also be used in monomolecular sensing technology and/or ultra-trace element detection technology, and can be used as a buffer reagent for improving the stability of detection.
G01N 27/26 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables électrochimiquesRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en utilisant l'électrolyse ou l'électrophorèse
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
A DNA polymerase and the use thereof. The DNA polymerase is: 1) a protein having an amino acid sequence as shown in SEQ ID NO: 1 or 2; or 2) a protein having an amino acid sequence that has 80% or more, more preferably 90% or more, and further preferably 95% or more homology to the amino acid sequence as shown in SEQ ID NO: 1 or 2, and having a DNA polymerase activity. The DNA polymerase has a relatively high amplification rate, a relatively good thermal stability, and 5'-3' polymerization activity and 3'-5' exonuclease activity. During DNA amplification, the DNA polymerase can be used to perform DNA amplification at a relatively high PCR extension rate, thus being able to better meet market demand, and having a great application potential.
Disclosed in the present disclosure are a focusing method and apparatus based on linear array scanning, a storage medium, and an electronic device. The method is applied to a biological sample scanning system, and the biological sample scanning system at least comprises: a camera and an objective lens. The method comprises: acquiring spatial position information of an anchor point corresponding to a sample to be scanned, wherein the spatial position information of the anchor point represents the position of a focused target objective lens; on the basis of the spatial position information of the anchor point, determining curved surface information corresponding to the anchor point, wherein the curved surface information corresponding to the anchor point at least comprises target objective lens positions of a plurality of sampling points obtained by sampling the spatial position information of the anchor point; acquiring the current objective lens position corresponding to the current scanning point in the sample to be scanned; and on the basis of the current objective lens position and the curved surface information corresponding to the anchor point, adjusting the current objective lens position to carry out focusing operation on the objective lens. By means of the described solution, the present application improves the focusing efficiency of the objective lens.
H04N 3/14 - Détails des dispositifs de balayage des systèmes de télévisionLeur combinaison avec la production des tensions d'alimentation par des moyens non exclusivement optiques-mécaniques au moyen de dispositifs à l'état solide à balayage électronique
54.
NUCLEIC ACID POLYPEPTIDE COMPLEX AND USE THEREOF IN PEPTIDE SEQUENCING
Provided are a nucleic acid polypeptide complex and the use thereof in peptide sequencing. The nucleic acid polypeptide complex comprises a first nucleic acid fragment, a polypeptide to be tested and a composite nucleic acid fragment which are connected in sequence, wherein the composite nucleic acid fragment comprises a nucleic acid sequence capable of forming a hairpin structure; after the hairpin structure is formed, the composite nucleic acid fragment comprises a double-stranded nucleic acid and a single-stranded nucleic acid, one end of the single-stranded nucleic acid is connected to the double-stranded nucleic acid, and the other end of the single-stranded nucleic acid is connected to the polypeptide to be tested. The detection of any polypeptide sequence can be achieved by means of guiding the polypeptide with different charges by the nucleic acid sequence to pass through the nanopore.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
55.
COMBINED ELEMENT FOR SCREENING APTAMER AND USE THEREOF, AND HIGH-THROUGHPUT SCREENING METHOD FOR APTAMER
A high-throughput screening method for an aptamer, and a use. An implementation route is divided into three parts, a first part is construction of a library of a pre-enriched aptamer and sequencing same, a second part is generation of an aptamer cluster on a slide, and a third part is combination of a target protein and the aptamer cluster. Provided is a method for high-throughput rapid detection of an aptamer. The method has the beneficial effects: sequencing time, sequence synthesis and affinity re-verification time are saved, the affinity verification costs are greatly saved, rapid screening of aptamers can be promoted, and the actual use of aptamers is promoted along with the increase in the types and numbers of aptamers. The existing platform BGISEQ 500 of Beijing Genomics Institute can be used to grow an aptamer cluster on a chip by using DNB as a template, and the use of sequencers is expanded by integrating sequencing, sequence synthesis and affinity identification.
An engineered biological containment system, comprising: a first nucleic acid, the first nucleic acid comprising at least a fragment portion of a first essential gene; a second nucleic acid, the second nucleic acid comprising at least a fragment portion of a second essential gene; a third nucleic acid, the third nucleic acid activating the transcription of the first nucleic acid; and a fourth nucleic acid, the fourth nucleic acid encoding a non-natural amino acid encoding tool. The second nucleic acid comprises one or more nucleotide sequences encoding a first termination codon and optionally a nucleotide sequence located at the end position of a second-essential-gene encoding region and encoding a second termination codon.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
C12N 15/65 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression utilisant des marqueurs
Provided in the present invention are a method for preparing a strand-specific library for the rapid detection of various types of RNAs, and a high-throughput sequencing method. A polyadenylic acid (poly A) tail is artificially added at the 3' terminal of various RNAs by using a poly A polymerase, and a single-stranded cDNA is synthesized using a poly-deoxythymidine ribonucleotide primer with deoxyuridine under the action of a reverse transcriptase. The obtained single-stranded cDNA molecule is subjected to a series of reactions, and the reaction product is finally amplified by means of PCR to obtain a strand-specific library of various types of RNAs. The library sample can be subjected to on-machine sequencing.
The present invention provides an underwater sequencer. The underwater sequencer comprises: a sample extraction device, used to extract an underwater microbial sample; a library construction device, an input port of the library construction device being connected to an output port of the sample extraction device, the library construction device being used to construct a gene library of the microbial sample; and a sequencing device, an input port of the sequencing device being connected to an output port of the library construction device, the sequencing device being used to perform gene sequencing on the gene library. Aiming at the relevant needs of underwater sequencing, the present invention integrates an extraction device, a library construction device, and a sequencing device, can automatically perform nucleic acid extraction, library construction, and sequencing without human intervention, and is easy to operate. The integrated underwater sequencer also has the advantages of small size and low power consumption.
Disclosed in the present invention is a method for repeated utilization of a microarray chip, the method comprising the following steps: 1) using a strong alkali or protease to treat a microarray chip which fails in quality control; and 2) re-preparing a new microarray chip from the microarray chip treated in step 1), so as to realize repeated utilization of the microarray chip. In the present invention, a strong alkali or protease is used to treat an SC which fails in quality control, and an FC is prepared from the SC; and protease is used to treat an SC which fails in quality control, and a new SC is re-prepared from the SC. By means of the present invention, a microarray chip which fails in quality control is re-utilized to reduce the cost of raw materials and the sequencing cost of the chip.
Provided in the present invention is a nanopore sequencing method using a closer. The method of the present invention enables a sequencing library to only bind within the nanopore capture range, ensuring that the library is efficiently captured by the nanopores during sequencing, thus improving the utilization efficiency of the library.
Disclosed in the present disclosure are a biochip scanning method and apparatus, and a storage medium and an electronic device. The method comprises: in response to a chip selection instruction, determining, from among a plurality of biochips, a chip to be scanned; parsing a chip scanning instruction to determine a target focusing mode; determining a scanning parameter corresponding to the target focusing mode; on the basis of the scanning parameter, performing a scanning operation on said chip, so as to obtain scanning data; and in response to a scanning end instruction, acquiring scanning data that is generated after the scanning operation, and generating a target scanning image on the basis of the scanning data.
Disclosed in the present invention are a sub-sequence search method for a gene sequence, a comparison method, a system, and a device. The sub-sequence search method comprises: acquiring a preset number of pieces of sequencing data of a gene sequence to be searched; sequentially acquiring, from a reference gene sequence, base data of a search interval corresponding to each piece of sequencing data, and performing a search on the basis of an acquisition result, wherein the search interval corresponding to each piece of sequencing data is determined on the basis of a base sequence to be searched of each piece of sequencing data; and repeatedly acquiring base data of a search interval of the next search, and performing a search on the basis of an acquisition result, so as to obtain a target sub-sequence, wherein the search interval of the next search is determined according to the next base to be searched of each piece of sequencing data and a search interval of the previous search. In the search method in the present invention, base data of a search interval is pre-fetched, and by means of batch processing of sequencing data, the calculation of the other sequencing data can be processed when waiting for a pre-fetching result, thereby improving the efficiency of a gene search.
Disclosed in the present invention is a chip substrate preparation method based on a glass sheet. The chip substrate preparation method comprises the following steps: making the surface of a glass sheet carry an amino, and reacting a coupling reagent having an azide group with the amino on the surface of the glass sheet, so as to form an amide bond. Further disclosed in the present invention is a chip preparation method. On the basis of the chip substrate preparation method, the chip preparation method comprises the following steps: reacting a 5'-end-modified probe with the azide group of the coupling reagent, and combining the probe on the surface of the glass sheet. In the present invention, the glass sheet is used to replace a silicon wafer, thereby reducing the cost of the chip raw material, also ensuring the number and quality of the probes of the chip, and improving the use stability of the chip.
A scanning imaging system (100) for a biochip, comprising a damping module (1), an objective table (2) and an optical engine module (3), wherein the damping module (1) comprises a damping bottom plate (11) and optical engine supporting frames (12) located on opposite sides of the damping bottom plate (11); in a first direction (z), the height of the optical engine supporting frames (12) is greater than the thickness of the damping bottom plate (11); the first direction (z) is perpendicular to a plane where the damping bottom plate (11) is located; the objective table (2) is located on the damping bottom plate (11) and is configured to carry a biochip (7); the objective table (2) can move in a second direction (x) and a third direction (y); in the second direction (x) and the third direction (y), the maximum moving distance of the objective table (2) is at least 20 cm; the second direction (x) and the third direction (y) are orthogonal to each other and are parallel to the plane where the damping bottom plate (11) is located; the optical engine module (3) is supported by two optical engine supporting frames (12); the optical engine module (3) covers an area where the damping bottom plate (11) is located, and is not in contact with the objective table (2); and the optical engine module (3) comprises an objective (32) and an optical imaging unit, and is configured to perform scanning imaging on the biochip (7).
G01Q 30/18 - Moyens pour protéger ou isoler l'intérieur d'une enceinte d'échantillonnage contre les conditions ou les facteurs environnementaux externes, p. ex. les vibrations ou les champs électromagnétiques
65.
ORGANIC SILICON COMPOSITE STRUCTURE, PREPARATION METHOD, AND MICROFLUIDIC CHANNEL STRUCTURE OF ORGANIC SILICON COMPOSITE STRUCTURE
The present invention provides an organic silicon composite structure, a preparation method, and a microfluidic channel structure of the organic silicon composite structure. The organic silicon composite structure comprises a non-silicon substrate layer, an intermediate layer, and an organic silicon layer; two surfaces of the intermediate layer are respectively bonded with the non-silicon substrate layer and the organic silicon layer; and the intermediate layer is an inorganic silicon layer. The intermediate layer is respectively bonded with the non-silicon substrate layer and the organic silicon layer, so that bonding between the non-silicon substrate layer and the organic silicon layer is achieved; moreover, due to a high-temperature organic reagent being not used to soak the non-silicon substrate layer during bonding, the material mechanical properties and the stability of the surface properties of the non-silicon substrate layer are kept, and the added intermediate layer basically does not affect the material mechanical properties of a substrate. An apparatus used during manufacturing is a common apparatus in a clean room for producing a semiconductor device, the operation is simple and direct, and the success rate is high.
B32B 5/00 - Produits stratifiés caractérisés par l'hétérogénéité ou la structure physique d'une des couches
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
G01N 37/00 - Détails non couverts par les autres groupes de la présente sous-classe
66.
PROTEIN FUNCTIONAL ANNOTATION METHOD AND APPARATUS, ELECTRONIC DEVICE, AND STORAGE MEDIUM
The present disclosure provides a protein functional annotation method and apparatus, an electronic device, and a storage medium. The method comprises: separately acquiring a sequence feature, a pre-trained sequence feature, and a preset annotation feature on the basis of a protein sequence; performing feature extraction on the preset annotation feature to obtain a first learning feature; performing feature extraction on the sequence feature and the pre-trained sequence feature to obtain a second learning feature; fusing the first learning feature and the second learning feature to obtain a first fused feature; and processing the first fused feature to obtain protein functional annotation information. Compared with the related art, the present disclosure relates to acquiring a sequence feature, a pre-trained sequence feature, and a preset annotation feature on the basis of a protein sequence, and fusing the three different aspects of features, such that protein functional annotation information obtained is more accurate.
Disclosed in the present invention are a DNA sequence library for continuous data storage, a method for continuous data storage, and a corresponding reading method and reading system. The reading method comprises: 1) capturing a DNA sequence in a DNA sequence library using a capture localization region, wherein the capture localization region corresponds to spatial information of continuous data; and 2) performing sequencing on a data region of the DNA sequence, wherein the data region corresponds to continuous data content, and restoring the continuous data content as the sequencing proceeds. The present invention innovates a reading mode for DNA data storage to realize instantaneous decoding, and solves the problem of low information reading efficiency in DNA data storage, thereby increasing the possibility of large-scale application of DNA data storage.
Disclosed are a method and apparatus for DNA storage encoding/decoding and rules thereof. The method comprises: executing single-molecule sequencing on a reference sequence, and acquiring actual sequencing data of the single-molecule sequencing; comparing the actual sequencing data with reference data of the reference sequence, counting the frequency, in the actual sequencing data, of sequencing errors of each sequence segment having a length of k, and calculating the proportion, in the actual sequencing data, of sequencing errors of each sequence segment having a length of k, that is, the error rate; and performing removal by using a sequence segment, the error rate of which exceeds a threshold, as a limiting condition. In the method of the present invention, the steps of DNA storage encoding/decoding are simplified, the time sequence of the steps is eliminated by using a threshold, and the complexity of data processing is reduced.
Provided in the present application are a cell type annotation method and apparatus, and a device and a storage medium. The method comprises: clustering cell sequencing data to obtain a plurality of cell populations; analyzing a differential high-expression component list of each cell population, wherein the differential high-expression component list includes differential high-expression components (the components refer to genes or proteins) in the cell population which are sequenced according to specificity scores in descending order; and for each cell population, according to the sequence of preset marker components corresponding to cell types in the differential high-expression component list of the cell population, determining the probability of the cell population belonging to each cell type, and determining a corresponding cell type with the highest probability to be the cell type to which the cell population belongs, wherein the sequence of the marker components corresponding to the cell types in the differential high-expression component list of the cell population is positively correlated with the probability of the cell population belonging to the cell type. In the present solution, the effect of a marker component on a cell type to which a cell population belongs is correspondingly enhanced or reduced according to the magnitude of the specificity of the marker component in the cell population, thereby improving the accuracy of an annotation result.
The present invention provides a preparation method for an extrachromosomal circular DNA library, a sequencing method, a kit, and a use thereof. The preparation method comprises: extracting total DNA in a sample and digesting linear DNA in the total DNA to obtain a digestion product, the digestion product comprising extrachromosomal circular DNA; directly carrying out rolling circle amplification on the digestion product without purification, to obtain an amplified product; and directly carrying out library preparation on the amplified product without purification, to obtain an extrachromosomal circular DNA library.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
71.
METHOD, APPARATUS, AND SYSTEM FOR DATA ACQUISITION
Embodiments of the present disclosure provide a method, apparatus, and system for data acquisition, which relate to the technical field of data processing. The method comprises: sectioning a biological tissue sample, and performing time-and-space omics sequencing on the sections to obtain expression level data; performing image acquisition on the sectioned biological tissue sample to obtain image data; performing registration on the expression level data and/or the image data to generate an outer-layer contour and/or a partition contour of the biological tissue sample; and generating three-dimensional display data for the biological tissue sample according to the outer-layer contour and/or the partition contour. The present disclosure provides a whole set of apparatus and method for data acquisition, and a registration method for expression level data and tissue section image data of time-and-space omics sequencing. Aiming at in situ capture time-and-space omics technology, combined with optical imaging information and assistance of rigid and elastic registration of continuous tissue sections, three-dimensional reconstruction is achieved; therefore, auxiliary visual information positioning of inner and outer tissue contours is obtained, and time-and-space omics data with large size and high resolution is effectively processed and displayed.
G06T 7/33 - Détermination des paramètres de transformation pour l'alignement des images, c.-à-d. recalage des images utilisant des procédés basés sur les caractéristiques
72.
DATA PROCESSING METHOD AND APPARATUS FOR IMAGE REGISTRATION, AND ELECTRONIC DEVICE
The present application relates to a data processing method and apparatus for image registration, and an electronic device, and relates to the technical field of data processing. The method comprises: firstly acquiring a first image and a second image to be registered; then registering the first image and the second image; then inputting the registered first image and second image into a machine learning model for calculation to obtain deformation field information, wherein the machine learning model is obtained by performing model training in advance according to image registration data of a sample image, and the image registration data comprises: the sample image and a deformed and corrected sample image; and determining corresponding coordinate information after image registration according to the deformation field information. The technical solutions of the present application solve the problem of elastic registration of multi-modal images, are easy to implement, and can improve the accuracy of image registration.
The present disclosure provides a spatiotemporal transcriptomics slice alignment method and apparatus. The main technical solution comprises: obtaining two adjacent slices, wherein each slice comprises at least one type of expression level matrixes, and each expression level matrix comprises spots of a plurality of pieces of spatiotemporal transcriptomics expression level data; respectively determining weights corresponding to the same type of spots in each slice, wherein the same weight is assigned to the same type of spots, and weights assigned to different types of spots are different (102); and according to the weight corresponding to each type of spots and a preset regularization coefficient, respectively calculating probability transition matrixes to obtain an alignment score of each spot in the expression level matrix corresponding to each category on the two slices (103). Compared with the related art, the same weight is assigned to the same type of spots in each slice, and the weights assigned to different types of spots are different, so that the accuracy of the probability transition matrixes obtained on the basis of weight calculation is improved, and the accuracy of alignment realized on the basis of the probability transition matrixes is thus improved.
G16B 20/30 - Détection de sites de liaison ou de motifs
G06T 7/33 - Détermination des paramètres de transformation pour l'alignement des images, c.-à-d. recalage des images utilisant des procédés basés sur les caractéristiques
74.
CELL CONTOUR RENDERING METHOD, APPARATUS AND SYSTEM
A cell contour rendering method, comprising: acquiring slice cell data, and performing segmentation processing on the slice cell data, so as to obtain segmented data; on the basis of the slice cell data, performing subdivision processing and/or area-reduction processing on contour points of cells in a slice, so as to generate multi-layer fineness vertex index data; writing the multi-layer fineness vertex index data into corresponding segmented data, so as to generate multi-layer fineness data to be subjected to rendering; and performing cell contour rendering according to the data to be subjected to rendering.
Disclosed are a registration method and apparatus for spatio-temporal transcriptome slices, an electronic device, and a storage medium. The method comprises: acquiring each target spot of a target slice, and respectively acquiring, in a probabilistic transfer matrix corresponding to an adjacent slice, an index vector of a spot having the maximum probability transfer value; performing rigid registration on the target slice on the basis of the probabilistic transfer matrix to obtain a registered target slice; calculating a deformation field of the registered target slice on the adjacent slice according to coordinates of the spot on the adjacent slice corresponding to the index vector and coordinates of the target spot; and respectively finding change values of coordinates in deformation fields of two adjacent slices, and registering the coordinates of each spot of the target slice by using the change values. After rigid registration is performed on the target slice, the deformation of the slice is registered to restore the real shapes of a plurality of slices in an organism, thereby further improving the accuracy of a registration result.
A deep reactive ion etching method of organic silicon, comprising: S110, providing an organic silicon substrate, and forming a patterned mask layer on the surface of the substrate; S120, placing into an etching system the organic silicon substrate on which the mask layer is formed, setting etching parameters, and introducing etching gas to etch the organic silicon substrate; and S130, removing the mask layer to obtain an organic silicon material subjected to ion etching. By selecting the combination of etching gases and accurately controlling the flow of the etching gases, an etching precision, an etching depth-to-width ratio, and an etching speed which are far higher than those obtained by using existing means can be obtained.
A property restoration method for a flexible microstructure having a high aspect ratio. The method comprises: placing a flexible microstructure substrate (2) in a first property restoration solvent for ultrasonic treatment, wherein the first property restoration solvent is used for immersing the flexible microstructure substrate (2); taking the flexible microstructure substrate (2) out of the first property restoration solvent, and transferring the flexible microstructure substrate (2) into a chamber containing a second property restoration solvent (3), wherein the second property restoration solvent (3) is used for immersing the flexible microstructure substrate (2); introducing a supercritical fluid to replace the second property restoration solvent (3) in the chamber; and totally replacing the second property restoration solvent (3) and then discharging the supercritical fluid. The flexible microstructure substrate (2) soaked in the first property restoration solvent is ultrasonically cleaned, so that the flexible microstructure substrate (2) that has agglomerated, deformed or collapsed can be separated. The second property restoration solvent (3) for soaking the flexible microstructure substrate (2) is then replaced with the supercritical fluid (6), and the stress accumulated on the flexible microstructure substrate (2) is released, so that the flexible microstructure substrate (2) is dried and restored in property. Thus, it is possible to prepare a flexible microstructure substrate (2) having a high aspect ratio in a mold forming mode.
A biosensor chip packaging structure (100), a PCB (201), and a micro-fluidic detection apparatus. The biosensor chip packaging structure comprises: a sensor chip (1), an integrated circuit chip (2) and a substrate (3), wherein the sensor chip (1) is used for being in contact with an external substance and converting a measured parameter into a measurement signal; the integrated circuit chip (2) is used for processing the measurement signal; and the sensor chip (1) and the integrated circuit chip (2) are independently provided on the substrate (3), and the sensor chip (1) and the integrated circuit chip (2) are electrically connected by means of the substrate (3). In the structure of biosensor chip packaging, the scrapping of a sensor chip does not affect an integrated circuit chip, thereby decreasing a scrap rate, and reducing the process difficulty; and the sensor chip can be subjected to measurement or maintenance independently, thereby improving the economical efficiency of maintenance.
B81B 7/02 - Systèmes à microstructure comportant des dispositifs électriques ou optiques distincts dont la fonction a une importance particulière, p. ex. systèmes micro-électromécaniques [SMEM, MEMS]
B81C 1/00 - Fabrication ou traitement de dispositifs ou de systèmes dans ou sur un substrat
A focusing method and system. The focusing method comprises: acquiring, captured by a photodetector (8), an electrical signal corresponding to a light spot (300) reflected by a surface of an object (7) to be measured; wherein the photodetector (8) comprises first pixels (100) and second pixels (200), an electrical signal corresponding to a first pixel (100) being a first electrical signal, and an electrical signal corresponding to a second pixel (200) being a second electrical signal; calculating the sum of a first electrical signal and a second electrical signal, to act as a sum signal; determining whether the sum signal is greater than or equal to a preset threshold; and in response to the sum signal being greater than or equal to the preset threshold, using a first preset relationship to determine a current amount of defocus, and using a second preset relationship to determine a speed of an objective lens (6) corresponding to the current amount of defocus, so as to control the objective lens (6) to move and focus at said speed.
Provided are a method for preparing a nucleic acid-polypeptide complex, a nucleic acid-polypeptide complex prepared by the method, and a use of the method. The method for preparing the nucleic acid-polypeptide complex can couple one or more polypeptides having different lengths and sequences with one or more nucleic acids, and the method has a wide application range, and has the advantages of high yield and easy purification of products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Chemical reagents, other than for medical or veterinary
purposes; chemical preparations for scientific purposes,
other than for medical or veterinary use; biochemical
preparations for scientific purposes; biological
preparations, other than for medical or veterinary purposes;
test paper, chemical; biological preparations for use in
cell cultures, other than for medical or veterinary
purposes; chemical substances for analyses in laboratories,
other than for medical or veterinary purposes; chemical
preparations for analyses in laboratories, other than for
medical or veterinary purposes; diagnostic reagents and
preparations, except for medical or veterinary use;
biological tissue cultures, other than for medical or
veterinary purposes. Biological preparations for medical purposes; medical
diagnostic reagents for the analysis of immune system;
preparations for detecting genetic predispositions for
medical purposes; reagents for use in medical genetic
testing; reagent paper for medical purposes; diagnostic
preparations for medical purposes; chemical preparations for
medical purposes; enzyme preparations for medical purposes;
preparations for detecting mutation in prion genes for
medical purposes; diagnostic biomarker reagents for medical
purposes. Computer software, recorded; computer software for database
management; data processing apparatus; laboratory devices
for detecting genetic sequences; laboratory equipment,
namely, protein sequence analysis apparatus; DNA
microarrays; nucleic acid sequencers used for analyzing
nucleic acids in scientific research; laboratory apparatus
and instruments, namely, gene analyzers for genome
information; laboratory chemical reactors; laboratory
instrument for the detection of pathogens and toxins in a
biological sample for research use; biochips; DNA chips;
testing apparatus not for medical purposes. Testing apparatus for medical purposes; diagnostic apparatus
for medical purposes; apparatus for use in medical analysis;
apparatus used in implementing diagnosis tests designed to
detect the abnormal prion protein; apparatus for blood
analysis; apparatus for DNA and RNA testing for medical
purposes; medical apparatus and instruments; probes for
medical purposes; genetic testing apparatus for medical
purposes; gas chromatography apparatus for medical purposes.
82.
SAMPLE SORTING STRUCTURE, SAMPLE SORTING METHOD AND RELATED DEVICE
A sample sorting structure (100), comprising: a centrifugal chamber assembly (101), the centrifugal chamber assembly (101) having a centrifugal inner cavity (101a); a rotation driving assembly (102) for driving the centrifugal chamber assembly (101) to rotate; a spindle (103), the spindle (103) being synchronously rotatably connected to the centrifugal chamber assembly (101), and one end of the spindle (103) extending to the centrifugal inner cavity (101a) and being movable relative to the centrifugal inner cavity (101a); an extrusion platform (104) fixed at the end of the spindle (103) extending to the centrifugal inner cavity (101a), the extrusion platform (104) being used for loading a sample bag to be sorted, and is driven by the spindle (103) to move close to or away from the end of the centrifugal inner cavity (101a) used for extruding the sample bag to be sorted; and a horizontal pushing driving assembly (105) for driving the spindle (103) to move in the centrifugal inner cavity (101a), the horizontal pushing driving assembly (105) being rotatably connected to the spindle (103). The sample sorting structure uses an automatic mode of extruding and sorting while centrifuging, improving sample sorting efficiency, making it easier to completely discharge samples, improving sample utilization, and reducing costs. Further disclosed are a sample sorting method based on the sample sorting structure, and a related device.
A sorting fluidic system, a cell sorting system, a sorting instrument, a fluid sorting method and a cell sorting method. The sorting fluidic system comprises a centrifugal mechanism, a pipeline consumable and a pipeline timing control assembly; the centrifugal mechanism is used for holding a sample holding member, a rotary connecting member separately communicated with the sample holding member and the pipeline consumable is mounted on the centrifugal mechanism, and the centrifugal mechanism drives liquid in the sample holding member to achieve centrifugal layering and presses liquid of a corresponding layer into the pipeline consumable; and the pipeline timing control assembly is mounted on the pipeline consumable and used for controlling the liquid entering the pipeline consumable to flow according to preset timing and a preset path. According to the provided sorting fluidic system, a target cell is directly obtained, and a cell contamination risk is reduced. In addition, the sample holding member is pressed by means of the centrifugal machine to press the layers into the pipeline consumable, so that all the layers in the sample holding member are pumped out without independently providing a pump, thereby simplifying the structure of the system.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A micro-droplet screening device, and a system. The micro-droplet screening device comprises an upper computer (1), an optical signal detection apparatus (2), a signal processor (3), and an electric screener (4). The optical signal detection apparatus (2) is used to acquire an optical signal of a droplet to be measured, and convert the optical signal into an electrical signal of the droplet to be measured. The signal processor (3) is electrically connected to the optical signal detection apparatus (2), the signal processor (3) being used to receive and process the electrical signal of the droplet to be measured, and send a screening instruction to the electrical screener (4) according to the strength of the electrical signal. The electric screener (4) is electrically connected to the signal processor (3), the electric screener (4) controlling the droplet to be measured in a microfluidic sorting chip (5) to deflect to the a corresponding flow channel according to the screening instruction, so as to complete screening of the micro-droplet to be measured. The upper computer (1) is electrically connected to the signal processor (3), the upper computer (1) being used to issue a screening instruction and configure a related screening parameter. Provided is a highly integrated and automated micro-droplet screening device, which greatly improves the convenience of microdroplet screening.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A flexible neural probe (100). The flexible neural probe (100) comprises a flexible probe body and a monitoring electrode mechanism. The flexible probe body comprises an air pressure control layer (200) and an electrode integration layer (300), which are arranged in a stacked manner, wherein at least one air chamber (400) is formed in the air pressure control layer (200). The flexible probe body has a tip; and the monitoring electrode mechanism is arranged on the electrode integration layer (300) and the monitoring electrode mechanism comprises at least one electrode (700), which is located on the tip of the flexible probe body. The air pressure in the air chamber (400) is configured to be adjustable, such that the flexible probe body deforms and changes the monitoring position of the electrode (700).
Provided are a method for constructing a sequencing library, a sequencing library, a sequencing method, and a kit for constructing a sequencing library. The method for constructing a sequencing library comprises: performing, under the action of an exonuclease, a digestion treatment on a sample to be tested which is linked to a linker, so as to obtain a target sequencing library.
Provided in the present invention are a porin monomer, a porin, a mutant thereof and the use thereof. The porin monomer comprises: (a) a protein composed of an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein mutant, wherein the amino acid sequence of the protein mutant is subjected to substitution, deletion and/or addition of one or several amino acids at at least one of the following positions in the amino acid sequence as shown in SEQ ID NO: 1: position 63, position 64, etc., and the protein mutant has the function of forming a pore channel structure via polymerization; or (c) a porin monomer having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% identity to the protein of (a) or (b), and having the function of forming a pore channel structure via polymerization. By means of the present invention, the problem in the prior art of the poor stability of a pore channel of the porin can be solved; and the present invention is applicable to the field of single-molecule sequencing.
The present disclosure relates to the technical field of spatiotemporal transcriptome data. Disclosed are a batch effect removal model training method and apparatus, and a batch effect removal method and apparatus. The batch effect removal model training method comprises: inputting a gene expression matrix for training into a neural network feature coding module to obtain a first processing result for training; inputting a cell space neighbor graph matrix for training and the first processing result for training into a graph network feature coding module to enhance neighbor center expression, so as to obtain a second processing result for training; splicing the first processing result for training and the second processing result for training to obtain a target gene expression matrix for training; and according to the target gene expression matrix for training, calculating a loss function of a batch effect removal model, and optimizing the batch effect removal model on the basis of the loss function. By comprehensively analyzing gene data of cells and cell space neighbor data, a batch effect between cell data is removed, and distribution of cell data is unified as much as possible, thereby realizing batch effect removal of spatiotemporal transcriptome data.
G06F 30/27 - Optimisation, vérification ou simulation de l’objet conçu utilisant l’apprentissage automatique, p. ex. l’intelligence artificielle, les réseaux neuronaux, les machines à support de vecteur [MSV] ou l’apprentissage d’un modèle
89.
POLYPEPTIDE HAVING DNA POLYMERASE ACTIVITY AND USE THEREOF
Provided are a polypeptide having DNA polymerase activity, the polypeptide comprising an amino acid sequence having at least 80% identity with SEQ ID NO: 1 or a polymerase active fragment or domain thereof, as well as a corresponding isolated nucleic acid, a vector and a host cell comprising the nucleic acid, a method for preparing the polypeptide, a composition or kit comprising the polypeptide or nucleic acid, and a method for nucleic acid amplification and sequencing by means of the polypeptide.
The present disclosure provides a chip quality inspection method and apparatus, an electronic device, and a storage medium. The method comprises: performing numbering processing on at least one field of view in each chip, and obtaining identification information of each field of view; on the basis of the identification information, separately acquiring a first channel image and a second channel image of the at least one field of view; performing image processing analysis on the at least one field of view on the basis of image features of the first channel image and the second channel image; and determining the quality of the chip according to a preset threshold combination and an image processing analysis result. According to embodiments of the present disclosure, a chip is divided into at least one field of view and then processed, and different channel images of each field of view are analyzed, so as to inspect the quality of the chip. In the prior art, parameters of a chip image are recorded mainly by means of manual sampling and observation, while in the present disclosure, a chip flaw can be automatically detected, so that detection accuracy can be improved and standardized detection can be achieved.
The present application relates to the technical field of data processing, and relates to a genotype imputation processing method and apparatus and an electronic device. The method comprises: first acquiring a sequencing file and a graph reference genome set, wherein the graph reference genome set is constructed in advance according to graph genomes, and the graph reference genome set comprises: reference sequences of the genomes and haplotype sequence information for multiple individuals; then calculating a likelihood value of a sequencing read length for each haplotype on the basis of the graph reference genome set; and performing, according to the likelihood value and the graph reference genome set, genotype imputation on the sequencing file that has undergone variant calling. By using the technical solution of the present application, the accuracy and imputation effect of genotype imputation can be improved.
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
Provided in the present invention are a nanopore sensor and the use thereof in sequencing. The nanopore sensor comprises: a membrane layer and a porin inserted into the middle of the membrane layer to form a pore channel, wherein when an electric field force is applied across the membrane layer, electrical conduction occurs in the pore channel. The porin comprises a porin monomer, and the porin monomer comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 2; or (b) a protein mutant, the amino acid sequence of the protein mutant being subjected to substitution, deletion and/or addition of one or more amino acids at at least one of the following sites of SEQ ID NO: 2: such as 80, 82 and 83, and the mutant having a function of forming a pore channel structure by means of polymerization; or (c) a protein which has at least 70% or above identity to the above-mentioned proteins, the mutant having the function of forming a pore channel structure by means of polymerization. Provided is the novel nanopore sensor capable of being used for nanopore sequencing, which is applicable to the field of single-molecule sequencing.
G01N 27/403 - Ensembles de cellules et d'électrodes
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
Provided in the present invention are a porin monomer, a porin, a mutant thereof and the use of same. The porin monomer comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein mutant the amino acid sequence of which is obtained by means of substitution, deletion and/or addition of one or several amino acids at at least one of the following sites in SEQ ID NO: 1: the 91st site, the 98th site, the 99th site, etc., the protein mutant having the function of forming pore structures by means of polymerization; or (c) a protein having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 99% identity to the protein of (a) or (b), and having the function of forming pore structures by means of polymerization. Provided is a novel nanopore sensor capable of being used for nanopore sequencing, which is applicable to the field of single molecule sequencing.
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
A nanopore sequencing method using a single-stranded specific nuclease, relating to the field of sequencing. The method can improve the sequencing flux, improve the capture efficiency of a target sequencing object, increase the number of holes (channels), increase an average read length of the target sequencing object, and/or improve the porin gating frequency.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
95.
LIBRARY ADAPTER DESIGN FOR IMPROVING SEQUENCING THROUGHPUT
Provided are a method for establishing a sequencing library, a sequencing library, a sequencing method, and a kit for constructing a sequencing library or for sequencing. The method for establishing a sequencing library comprises: digesting, under the action of exonuclease, a sample under test connected with an adapter to obtain a sequencing library. The 5' end of the adapter does not have phosphorylation modification, and the 3' end of the adapter is connected to the 5' end of said sample.
The present invention relates to the field of nanopore sequencing, and in particular to a novel nanopore protein BCP52, and a mutant and use thereof. According to the present invention, a novel nanopore protein BCP52 is found in a deep-sea metagenome, protein preparation and nanopore sequencing verification show that the nanopore protein BCP52 has the capability of being applied to nanopore sequencing, and optimization of the mutant of the nanopore protein BCP52 can improve the accuracy of nanopore sequencing.
Disclosed in the present invention are a helicase and a use thereof. The helicase has two tower domains and a PIN domain, and the two tower domains are located on the same side of a helicase three-dimensional structure. According to the technical solution of the present invention, a brand-new helicase BCH3X having a special helix characteristic domain is provided, and the helicase has good salt tolerance and stability, can have high unwinding activity in the case of a high salt content, can be used for nucleic acid control and characterization, and is applied to nanopore sequencing.
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
Disclosed are a helicase ToPif 1, and a preparation method therefor and a use thereof in high-throughput sequencing. The amino acid sequence of a ToPif 1 helicase mutant is as shown in SEQ ID NO. 1. The helicase can be used for controlling and characterizing a nucleic acid and is applied to nanopore sequencing.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C07K 14/35 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Mycobacteriaceae (F)
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
Disclosed in the present invention are a mutant of a Dda helicase, and a preparation method therefor and a use thereof in sequencing. The mutant undergoes, on an amino acid sequence as shown in SEQ ID NO: 4, one or more mutations selected from the following: (1) substituting a positively charged amino acid in the amino acid sequence with a neutral amino acid; (2) substituting a negatively charged amino acid in the amino acid sequence with a neutral amino acid; (3) truncating an amino acid in an N-terminal region in the amino acid sequence or substituting the amino acid in the N-terminal region in the amino acid sequence with a short-side-chain neutral amino acid; and (4) substituting, with a short-side-chain amino acid, an amino acid having large steric hindrance in a side-chain amino acid in the amino acid sequence. The mutant greatly improves the sequencing capability, and further promotion of nanopore sequencing work is facilitated.
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
100.
HELICASE AND PREPARATION METHOD THEREFOR AND USE THEREOF IN HIGH-THROUGHPUT SEQUENCING
Provided are a helicase and a preparation method therefor and a use thereof in sequencing. The amino acid sequence of the helicase is as shown by SEQ ID NO:1 or has at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the amino acid sequence as shown by SEQ ID NO:1. The helicase has high thermal stability, ATP hydrolysis activity and DNA unwinding activity, the DNA unwinding activity increases with increasing salt concentration, and the helicase can be used for control and characterization of nucleic acids, and can be applied to nanopore sequencing.
