The invention provides methods for the detection of molecular targets by digital PCR (dPCR) using a set of universal probes and target-specific tailed primers. Each target is amplified by a unique mixture of primers. The tailed amplicons anneal to a universal set of probes to detect the associated targets. Some targets are amplified using more than one tailed primer. Some targets are amplified using the same multiple tailed primers. In these embodiments, the primers are concentrated to produce a different number of amplicons for each tailed primer, resulting in a different probe-amplicon balance for each target. Two colors of fluorescence intensity are read and plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a universal set of probes in multiple assays provides for greater flexibility and throughput.
The invention provides methods for the detection of molecular targets by digital PCR (dPCR) using a set of universal probes and target-specific tailed primers. Each target is amplified by a unique mixture of primers. The tailed amplicons anneal to a universal set of probes to detect the associated targets. Some targets are amplified using more than one tailed primer. Some targets are amplified using the same multiple tailed primers. In these embodiments, the primers are concentrated to produce a different number of amplicons for each tailed primer, resulting in a different probe-amplicon balance for each target. Two colors of fluorescence intensity are read and plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a universal set of probes in multiple assays provides for greater flexibility and throughput.
The present invention relates to novel methods, compositions, kits and systems for the sensitive detection of biomarkers in immunoassays. Disclosed herein are reference materials and methods for monitoring the precision and accuracy of various immunodiagnostic testing methodologies in clinical laboratories.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 33/564 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour complexes immunologiques préexistants ou maladies auto-immunes
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
B01J 41/14 - Composés macromoléculaires obtenus par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 41/07 - Procédés utilisant des échangeurs organiques sous forme faiblement basique
B01J 47/014 - Procédés d'échange d'ions en généralAppareillage à cet effet dans lesquels les propriétés d’adsorption de l’échangeur d’ions sont utilisées, p. ex. récupération de protéines ou de composés macromoléculaires
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
Produits et services
Medical laboratory research instruments for testing autoimmune, infectious disease, and other specialty testing; Diagnostic apparatus for research laboratory use for testing autoimmune, infectious disease, and other specialty testing; Laboratory equipment, namely, trays and racks; Scientific laboratory instruments, namely, scientific laboratory workstations and associated computer software used for protein or immunoassay analysis in the fields of drug discovery, in-vitro diagnostics, and biomedical diagnosis; Downloadable software for use in the fields of clinical diagnostics Medical devices for use in diagnosing autoimmune, infectious disease, and other specialty testing; Diagnostic apparatus for medical purposes used in medical laboratories for testing autoimmune, infectious disease, and other specialty testing; Medical diagnostic apparatus for detecting autoimmune, infectious disease, and other specialty testing; Immunochemical testing apparatus for medical use
7.
METHODS, SYSTEMS, AND APPARATUSES FOR GENERATING AND DISPENSING EMULSION DROPLETS
Disclosed herein are novel methods, systems, and apparatuses for generating and dispensing emulsion droplets, which beneficially eliminates the use of microfluidics chips typically used in emulsion droplet generation, thereby reducing the cost and complexity of emulsion droplet generation; and improving the ease of performance, efficacy, and reliability of droplet-based assays. Disclosed herein are high-throughput methods of contacting liquid droplets with an oil to form emulsion droplets, and simultaneously transferring said emulsion droplets to an amplification vessel. Systems and apparatuses suitable to perform the methods of the invention are also provided. Droplets generated accordingly are suitable for a reaction e.g. an amplification reaction, and detection of one or more reaction characteristics or outputs. The method may be particularly useful for the formation of droplets required for amplification reactions such as dPCR.
Compositions for the detection of contaminant enzymes are provided. In particular, oligonucleotide primers are provided, wherein the primer is conjugated to a blocking moiety at its 3'end, wherein the blocking moiety comprises a cleavage site for an enzyme, and wherein cleavage at the cleavage site releases the blocking moiety and produces a 3' hydroxyl group on the oligonucleotide primer. Also provided is an oligonucleotide primer comprising a cleavable moiety at its 5' end, wherein the cleavable moiety is also conjugated to an oligonucleotide tail. Also provided are methods for making the disclosed oligonucleotide primers and methods for using the oligonucleotide primers to detect a contaminant enzyme in a sample.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
A61K 47/54 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique
10.
SINGLE-CELL SEQUENCING USING MULTIPLE TRANSPOSASE ADAPTERS
Methods and compositions are provided for generating cDNA reads from cDNA fragments comprising tagmentation with at least three transposases carrying different homoadaptor oligonucleotides with different end sequences.
Disclosed herein are novel target genomic regions and corresponding primers and probes for performing prenatal testing assays, and methods for performing prenatal testing assays which comprise estimation of foetal fraction, aneuploidy assays, and 22q microdeletion assays, to obtain aneuploidy scores and 22q microdeletion scores, and suitably foetal fraction adjusted aneuploidy scores, and foetal fraction adjusted 22q microdeletion scores. The methods may be particularly useful for NIPT or IPT applications.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical preparations in the nature of ion-exchange resins; Chemical preparations in the nature of ion-exchange resin membranes; Chromatography chemicals; Chromatography separation media for separation of a mixture into components Chromatography columns for laboratory use; Liquid chromatography apparatus for laboratory use; Scientific apparatus and instruments, namely, chromatography columns for use in purification in the laboratory and parts and fittings therefor; Chromatography columns for use in purification in the laboratory; Automatic ion-exchange chromatography apparatus for laboratory use
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/541 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec séparation du complexe immunologique de l'antigène ou de l'anticorps non liés faisant intervenir un réactif de précipitation faisant intervenir un double ou un deuxième anticorps
14.
METHODS AND SYSTEMS FOR TRAINING CLINICAL DIAGNOSTIC MODELS
This disclosure provides a novel approach to training clinical diagnostic models using synthetic data generated by an artificial intelligence (Al) model. The disclosed models demonstrate several advantages, including: reducing time, cost, and complexity associated with traditional data collection and labeling. Additionally, the disclosed models address privacy and data management issues and allow for the inclusion of rare or novel cases in the training set.
G01N 33/80 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir les groupes ou les types sanguins
A61B 5/145 - Mesure des caractéristiques du sang in vivo, p. ex. de la concentration des gaz dans le sang ou de la valeur du pH du sang
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
G16H 10/60 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients
Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
Methods and compositions are provided for detecting multiple barcodes in a partition for sequencing applications. Bead-specific barcoding oligonucleotides and/or detection oligonucleotides are used to introduce a bead-specific barcode sequence and a universal adapter sequence to target sequences from a single cell or nucleus. Also provided are beads comprising one or more bead-specific barcoding nucleic acids. In addition, the disclosure provides a plurality of partitions that include a single cell or nucleus or nucleic acids from a single cell or nucleus, beads comprising one or more bead-specific barcoding oligonucleotides, and a DNA polymerase.
Disclosed herein are cfDNA-like DNA fragments and broadly applicable methods to prepare cfDNA-like DNA fragments which produce a high yield and/or large quantities of cfDNA-like DNA fragments. The cfDNA-like DNA fragments produced according to the methods of the disclosure accurately capture the size distribution and diagnostic assay performance of natural cfDNA, and may therefore be used for assay methodological validation, internal assay quality control and external assay quality evaluation with high reproducibility and consistency.
Method of analysis. In the method, a microfluidic device defining a flow path extending from an inlet to an outlet may be selected. A sample-containing fluid may be introduced into the flow path via the inlet. Volumes of the sample-containing fluid may be isolated from one another on the flow path. A two-dimensional monolayer of the volumes may be imaged. The two-dimensional monolayer may be formed along the flow path between the inlet and the outlet.
B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
B29C 45/00 - Moulage par injection, c.-à-d. en forçant un volume déterminé de matière à mouler par une buse d'injection dans un moule ferméAppareils à cet effet
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
G01N 21/3563 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière infrarouge pour l'analyse de solidesPréparation des échantillons à cet effet
G01N 21/49 - Dispersion, c.-à-d. réflexion diffuse dans un corps ou dans un fluide
The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
B01L 9/00 - Dispositifs de supportDispositifs de serrage
B03C 5/00 - Séparation de particules des liquides dans lesquels elles sont dispersées, par effet électrostatique
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
C07K 14/20 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Spirochaetales (O), p. ex. Tréponème, Leptospira
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C40B 50/08 - Synthèse en phase liquide, c.-à-d. dans laquelle tous les blocs servant à créer la bibliothèque sont en phase liquide ou en solution au cours de la création de la bibliothèqueProcédés particuliers de clivage à partir du support liquide
G01N 33/532 - Production de composés immunochimiques marqués
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
B01J 20/289 - Phases reliées chimiquement à un substrat, p. ex. à de la silice ou à des polymères reliées par l'intermédiaire d'un espaceur
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
Disclosed is a system and method for dynamically adjusting analytical precision of a clinical diagnostic process. The system and method employ a clinical diagnostic analyzer that evaluates a sample and determines whether the resultant value, or mean value, is within a predetermined window or within a threshold of a predetermined value of a precision profile. If so, the analyzer automatically and dynamically determines a desired analytical precision and conducts additional testing of replicate samples to achieve a desired precision and reports the results to a user.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 15/00 - TIC spécialement adaptées aux rapports médicaux, p. ex. leur création ou leur transmission
A wide-spectrum analysis system. The system may comprise various components, including a stage, a detection module, and an optical relay structure. The stage may be configured to support a sample holder—a gel or blot, a PCR plate or microplate, sample chips, or a microfluidic device, among others—at an examination region. The detection module may be configured to detect light originating from one or more samples positioned in the sample holder. The detection module may be configured to detect light having wavelengths between about 200 nm and about 2000 nm, or subsets thereof. The optical relay structure may be configured to direct the output light from the examination region to the detection module. The system may further comprise an illumination module. Embodiments of the analyzer may be suitable for use with one or more of the following interrogation formats, among others: chemiluminescence, fluorescence, colorimetry, and spectrometry.
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical reagents for scientific and medical research purposes; diagnostic reagents for clinical or medical laboratory use; Polymerase Chain Reaction (PCR) and digital Polymerase Chain Reaction (dPCR) reagents for scientific and medical research purposes; reagents for amplification and detection of nucleic acids for scientific and medical research purposes; Kits comprising chemicals, reagents, enzymes, and buffer solutions for amplifying and labeling nucleic acids for scientific and research use; Reagent kits comprising biological protein, DNA primers, polymerase or buffers for scientific and medical research use; Kits comprising chemicals, reagents, enzymes, and buffer solutions for amplifying and labeling nucleic acids for scientific or research use; Kits consisting primarily of reagents and magnetic, polymeric, or gel beads for scientific research use purposes. Apparatus, namely, medical laboratory research instruments and scientific laboratory research instruments for preparation, handling, amplification, and analysis of samples containing nucleic acids; accessories in the nature of laboratory apparatus, namely, sample preparation containers and kits consisting primarily of sample preparation containers and reagents, and instruction manuals sold as a unit therewith, used to prepare laboratory samples for use in scientific and medical research, all in connection with the preparation, handling, amplification, and analysis of samples containing nucleic acids; scientific apparatus, instruments and equipment for scientific research, non-medical diagnostic analysis, chemical analysis, clinical analysis and industrial use, namely, DNA and RNA testing apparatus; scientific laboratory equipment and apparatus, namely, digital polymerase chain reaction (dPCR) apparatus, electrophoresis apparatus, gel electrophoresis apparatus, and thermal cyclers, for use in amplifying, labeling, and analyzing nucleic acids, and parts and consumable parts therefor, namely, tubes, pipette tips, plates, cartridges, holders, and containers for liquids and gels; Laboratory apparatus and instruments, namely, digital Polymerase Chain Reaction (dPCR) instruments for amplifying DNA using digital Polymerase Chain Reaction (dPCR) for use in scientific research; Scientific apparatus and instruments, namely, droplet generators for sample preparation of nucleic acids and droplet reader for amplifying and analyzing samples containing nucleic acids; Laboratory equipment and supplies, namely, polymerase chain reaction (PCR) systems comprised of computer hardware, downloaded and recorded computer software for operating polymerase chain reaction (PCR) instruments and analyzing and tabulating test results, thermocycler apparatus, polymerase chain reaction (PCR) kits comprising DNA microarrays, DNA probes and DNA assays.
Systems and methods for automating hands-free workflow of laboratory procedures. The system includes a wearable device with a display and a workflow controller in communication with the wearable device. The workflow controller can be configured to extract a workflow for a specified laboratory procedure from a workflow repository. The workflow can include a plurality of instructions arranged in a specified sequence. One or more instructions of the plurality of instructions can be sequential delivered to the wearable device for an operator to follow. The one or more instructions can include a visual indicator configured to be displayed within the display of the wearable device. The wearable device can be configured to receive voice commands and the workflow controller can be configured to navigate the workflow based on the voice commands. The wearable device can be used to scan codes and display additional information during workflow implementation.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 40/67 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement à distance
29.
OPTICAL BLACK PIXEL REFERENCE TO REMOVE IMAGE BIAS NOISE FOR WESTERN BLOT IMAGING
An imaging system dynamically computes an offset for an image based on an active region and a reference region of an image sensor used to generate the image. The imaging system applies the offset to pixels of the image. The imaging system further processes the image by performing flat-fielding, binning, or both.
H04N 25/633 - Traitement du bruit, p. ex. détection, correction, réduction ou élimination du bruit appliqué au courant d'obscurité en utilisant des pixels noirs optiques
H04N 25/46 - Extraction de données de pixels provenant d'un capteur d'images en agissant sur les circuits de balayage, p. ex. en modifiant le nombre de pixels ayant été échantillonnés ou à échantillonner en combinant ou en groupant les pixels
30.
OPTICAL BLACK PIXEL REFERENCE TO REMOVE IMAGE BIAS NOISE FOR WESTERN BLOT IMAGING
An imaging system dynamically computes an offset for an image based on an active region and a reference region of an image sensor used to generate the image. The imaging system applies the offset to pixels of the image. The imaging system further processes the image by performing flat-fielding, binning, or both.
H04N 25/633 - Traitement du bruit, p. ex. détection, correction, réduction ou élimination du bruit appliqué au courant d'obscurité en utilisant des pixels noirs optiques
H04N 25/59 - Commande de la gamme dynamique en commandant la quantité de charge stockable dans le pixel, p. ex. en modifiant le rapport de conversion de charge de la capacité du nœud flottant
H04N 25/76 - Capteurs adressés, p. ex. capteurs MOS ou CMOS
31.
CHROMATOGRAPHY RESIN HAVING AN ANIONIC EXCHANGE-HYDROPHOBIC MIXED MODE LIGAND
B01D 15/32 - Chromatographie en phase liée, p. ex. avec une phase normale liée, une phase inverse ou une interaction hydrophobe
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
B01J 20/289 - Phases reliées chimiquement à un substrat, p. ex. à de la silice ou à des polymères reliées par l'intermédiaire d'un espaceur
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Chemical reagents for scientific and medical research purposes; diagnostic reagents for clinical or medical laboratory use; reagents for amplification and detection of nucleic acids for scientific and medical research purposes; Assays and reagents for use in genetic research; diagnostic preparations for scientific or research use; diagnostic preparations for clinical or medical laboratory use; kits comprising chemicals, reagents, enzymes, and buffer solutions for amplifying and labeling nucleic acids for scientific and research use; Reagent kits comprising biological protein, DNA primers, polymerase or buffers for scientific and medical research use; Chemical, biochemical and biotechnological products for industrial and scientific purposes, namely, diagnostic preparations except for human or veterinary medical purposes, non-medical reagents, enzymes, oligonucleotides, and buffer solutions for scientific or research use for amplifying, labeling, and analyzing biopolymers and nucleic acids; Kits comprising chemicals, reagents, enzymes, and buffer solutions for amplifying and labeling nucleic acids for scientific or research use; Kits comprising chemicals for sample preparation, modification and manipulation of cells and for marking, separating, isolating, purifying, duplicating, sequencing and for the analysis of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular nucleic acids or biological or biochemical sample material, in particular chemicals for reagents, enzymes and buffer solutions for nucleic acid purification, enrichment, amplification, sequencing and analysis
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Reagents, and kits consisting primarily of reagents or of reagents and sample preparation containers, for use in scientific and medical research; reagents for scientific and research use for preparation, handling, amplification, and analysis of samples containing nucleic acids for use in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology, all in connection with the preparation, handling amplification, and analysis of samples containing nucleic acids.
35.
METHODS AND COMPOSITIONS FOR DECONVOLUTING PARTITION BARCODES
Methods and compositions for method of generating a population of captured target nucleic acids are provided. Exemplary methods can include contacting the plurality of oligonucleotides individually linked to a solid support with a sample comprising target nucleic acids having 3' ends and 5' ends, wherein target nucleic acids anneal to some of the free 3' ends of the oligonucleotides, and wherein there is an excess of oligonucleotides such that at least some oligonucleotides remain with free 3' ends; and then extending the target nucleic acid 3' end with a polymerase using the oligonucleotide as a template to form double-stranded portions that comprise a restriction enzyme recognition sequence; and then cleaving the restriction enzyme recognition sequence with a restriction enzyme to form released target nucleic acids having a single-stranded 5' end and a double-stranded 3' end and leaving oligonucleotides remain with free 3' ends uncleaved from the solid support; and optionally then separating the solid support and uncleaved oligonucleotides from the released target nucleic acids to form a solution of target nucleic acids.
Apparatus, namely, nucleic acid, including DNA and RNA, testing apparatus for medical diagnostic analysis and determination of medical conditions; Medical diagnostic apparatus and instruments, namely, Polymerase Chain Reaction (PCR) instrument to perform amplification and analyzation of nucleic acids; Medical apparatus and instruments, namely, droplet generators for sample preparation of nucleic acids and droplet readers analyzing samples containing nucleic acids; Medical devices and instruments, namely, a digital Polymerase Chain Reaction (dPCR) platform for use in measuring and quantifying DNA for medical diagnostic purposes; Medical diagnostic apparatus for the analysis of bodily fluids, tissues, biomolecules, DNA, antibodies, proteins, molecular material or cellular material; well plates for use as medical apparatus; cartridges for use as medical apparatus; Medical diagnostic kits consisting primarily of probes, buffers and reagents for use in analyzing samples containing nucleic acids.
Apparatus, namely, nucleic acid, including DNA and RNA, testing apparatus for medical diagnostic analysis and determination of medical conditions; Medical diagnostic apparatus and instruments, namely, Polymerase Chain Reaction (PCR) instrument to perform amplification and analyzation of nucleic acids; Medical apparatus and instruments, namely, droplet generators for sample preparation of nucleic acids and droplet readers analyzing samples containing nucleic acids; Medical devices and instruments, namely, a digital Polymerase Chain Reaction (dPCR) platform for use in measuring and quantifying DNA for medical diagnostic purposes; Medical diagnostic apparatus for the analysis of bodily fluids, tissues, biomolecules, DNA, antibodies, proteins, molecular material or cellular material; well plates for use as medical apparatus; cartridges for use as medical apparatus; Medical diagnostic kits consisting primarily of probes, buffers and reagents for use in analyzing samples containing nucleic acids
A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
Hybrid reverse transcriptases are provided that comprise a non-retroviral retrotransposon, or a fragment of the non-retroviral retrotransposon having reverse transcriptase activity, joined to a nucleic acid binding protein. Also provided are methods of using the hybrid reverse transcriptases to prepare a cDNA molecule library.
This disclosure provides methods of synthesizing fluorosurfactants and fluorosurfactants produced by the disclosed methods. These fluorosurfactants can be used in various protocols, such as PCR protocols.
The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
B01F 33/302 - Micromixeurs les matières à mélanger s'écoulant sous forme de gouttelettes
B01F 33/81 - Combinaisons de mélangeurs similaires, p. ex. avec des dispositifs d'agitation rotatifs dans plusieurs récipients
B01L 9/00 - Dispositifs de supportDispositifs de serrage
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
Methods and compositions are provided for preparing RNAs, from a single cell, for sequencing applications. Bead-specific barcoding oligonucleotides are used to introduce a beadspecific barcode sequence, a universal adapter sequence, and optionally one or more tail sequences to cDNA from the single cell. Also provided are plurality of partitions that include a single cell or nucleus or nucleic acids from a single cell or nucleus, beads comprising beadspecific barcoding oligonucleotides, a detection oligonucleotide, a reverse transcriptase, and a DNA polymerase.
A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.
The invention provides methods for the detection of molecular targets by digital PCR (dPCR) with a blocker for non-target molecules. Each target is provided with a unique mixture of probes. The blocker binds to a non-target molecule and blocks it from contributing to fluorescence from partitions. Two or more colors of fluorescence intensity are read, and two colors of fluorescence intensity are plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a blocker selects against the detection of non-target molecules and improves the resolution of radial multiplexing, allowing multiple targets to be detected.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The invention provides methods for the detection of molecular targets by digital PCR (dPCR) with a blocker for non-target molecules. Each target is provided with a unique mixture of probes. The blocker binds to a non-target molecule and blocks it from contributing to fluorescence from partitions. Two or more colors of fluorescence intensity are read, and two colors of fluorescence intensity are plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a blocker selects against the detection of non-target molecules and improves the resolution of radial multiplexing, allowing multiple targets to be detected.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.
The subject invention pertains to the detection of modifications to a polynucleotide by binding an affinity binding element to the nucleotide sequence. The affinity binding element can comprise a compound that binds to the modified nucleotide base and a nucleotide sequence that is complementary to the target nucleotide sequence, an oligonucleotide primer that is complementary to the target nucleotide sequence, or a nucleotide sequence complementary to a connector oligonucleotide. The invention provides methods of detecting one or more modifications to a nucleotide sequence. The invention further provides affinity binding elements and, optionally, primer oligonucleotides and/or probe oligonucleotides, and methods of using said affinity binding elements in assays to a detect modified base. The methods of the invention detect a modified nucleotide that are implicated in cancer, psychiatric disorders, or metabolic diseases.
Sample racks and removable partitions removably coupled to the body of the sample rack are provided to stably support sample containers received for sample analysis while maintaining proper alignment of each sample container. A partition can comprise at least two stackable segments, each having gripping flexures disposed to hold a sample container in the desired orientation during use. The sample rack can further include a retaining cap disposed over the partitions to hold them in place during use.
Systems including thermocyclers with dual vapor champers and methods of use of the same are described. The system can be used in performing automated Polymerase Chain Reaction (PCR). The system can include a thermocycler that can thermocycle samples in a PCR cartridge. The thermocycler can include a thermal element, and a first vapor chamber thermally coupled to the thermal element. The first vapor chamber can thermally couple to the PCR cartridge during thermocycling of the PCR cartridge. The system can include an imager and a processor communicatively coupled with each of the thermocycler and the imager. The processor can control the operation of each of the thermocycler and the imager to perform digital PCR. The processor can thermocycle the sample in the PCR cartridge with the thermocycler, and image the sample in the PCR cartridge with the imager.
Methods and compositions for deconvoluting partitions comprising multiple bead-specific barcodes are provided. The methods can involve for example in vitro transcription of detection oligonucleotides within partitions, which can be linked to bead-specific barcodes and sequenced.
Provided herein are methods of detecting fragmentation of an RNA transcript in a sample where the method includes using a first primer pair to amplify a first RNA sequence of the RNA transcript that is at most partially fragmented and a second primer pair to amplify a second RNA sequence of the RNA transcript where all or a portion of the second RNA sequence is fragmented into at least a first RNA fragment and a optionally second RNA fragment.
C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction
A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
G01N 15/01 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux spécialement adaptée aux cellules biologiques, p. ex. aux cellules sanguines
G01N 15/10 - Recherche de particules individuelles
G01N 15/1433 - Traitement du signal utilisant la reconnaissance d’image
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
A method of analysis for alleles of a target includes forming plural fluid volumes. Each of those volumes includes first and second primer pairs to amplify corresponding first and second alleles of a target. Each of those volumes also includes first and second reporters with corresponding first and second photoluminophores and providing corresponding primers of the corresponding first and second primer pairs. Each of the first and second reporters may include an oligomer that has a quencher and is configured to base-pair with the primer of the corresponding first and second primer pair. The first and second alleles may be amplified using the corresponding first and second primer pairs. The method detects photoluminescence and determines a level of each allele.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
This disclosure pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands are also disclosed. Similarly, methods of purifying proteins from a biological sample, source solution, or source liquid using the disclosed chromatography matrices are also provided.
B01J 41/08 - Utilisation d'une substance comme échangeur d'anionsTraitement d'une substance en vue d'améliorer ses propriétés d'échangeur d'anions
B01J 45/00 - Échange d'ions dans lequel se forme un complexe ou un chélateUtilisation d'une substance comme échangeur d'ions formant des complexes ou des chélatesTraitement d'une substance en vue d'améliorer ses propriétés d'échange d'ions formant des complexes ou des chélates
The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. The microscope is adjustable, with a structure that can move along range(s) of motion and degree(s) of freedom to allow for ease of access to samples, shielding of samples, and manipulation of a display apparatus.
The subject invention pertains to multimodal chromatography resins comprising anionic and cationic ligands on each resin particle and methods of synthesizing the multimodal chromatography resin by contacting a base with quaternary amines and/or tertiary amines on the surface of a substrate. The multimodal resins can be tuned by changing the ratios of cation to anions. The multimodal chromatography resins can be used for purifying proteins.
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01J 20/286 - Phases reliées chimiquement à un substrat, p. ex. à de la silice ou à des polymères
Provided herein is a chromatography media composed of a particulate substrate (such as beads or other particulate substrates) that is coated with hydroxyapatite (HA) and in which the particulate substrate used as the base upon which calcium phosphate (CaP) is formed is treated with heat to remove the substrate, convert the CaP to HA, and form the desired HA microstructure so as to form a templated porous HA substrate (TPHA substrate). Also provided is a chromatography media (optionally contained within a column) comprising a TPHA substrate (e.g., a plurality of TPHA particles). The TPHA particles are porous or macroporous and uses thereof.
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
Provided herein is a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with hydroxyapatite (IA). Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Thus this disclosure provides a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with I-IA. Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Methods of preparing the HA-coated substrate are also provided.
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
B01D 15/08 - Adsorption sélective, p. ex. chromatographie
B01J 20/28 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation caractérisées par leur forme ou leurs propriétés physiques
The subject invention pertains to multimodal chromatography resins comprising anionic and cationic ligands on each resin particle and methods of synthesizing the multimodal chromatography resin by contacting a base with quaternary amines and/or tertiary amines on the surface of a substrate. The multimodal resins can be tuned by changing the ratios of cation to anions. The multimodal chromatography resins can be used for purifying proteins.
B01J 43/00 - Échange d'ions amphotère, c.-à-d. utilisant des échangeurs d'ions comportant des groupes anioniques et cationiquesUtilisation d'une substance comme échangeur d'ions amphotèreTraitement d'une substance en vue d'améliorer ses propriétés amphotères d'échange d'ions
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
Provided herein is a chromatography media composed of a particulate substrate (such as beads or other particulate substrates) that is coated with hydroxypatite (HA) and in which the particulate substrate used as the base upon which calcium phosphate (CaP) is formed is treated with heat to remove the substrate, convert the CaP to HA, and form the desired HA microstructure so as to form a templated porous HA substrate (TPHA substrate). Also provided is a choromatography media (optionally contained within a column) comprising a TPHA substrate (e.g., a plurality of TPHA particles). The TPHA particles are porous or macroporous and uses thereof..
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
B01J 20/06 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des oxydes ou des hydroxydes des métaux non prévus dans le groupe
This disclosure relates to particulate hydroxyapatite (HA)-coated substrates, methods of making particulate HA-coated substrates, and methods of using particulate HA-coated substrates. Thus, the disclosure provides chromatography media composed of a particulate substrate (such as polymer beads or other particulate substrates) that is coated with HA. Also provided is a chromatography media (optionally contained within a column) comprising a HA-coated substrate (e.g., a plurality of beads or another particulate substrate that is coated with HA). The beads or particulate substrate can be a porous or non-porous substrate.
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
B01D 15/42 - Adsorption sélective, p. ex. chromatographie caractérisée par le mode de développement, p. ex. par déplacement ou par élution
This disclosure relates to particulate hydroxyapatite (HA)-coated substrates, methods of making particulate HA-coated substrates, and methods of using particulate HA-coated substrates. Thus, the disclosure provides chromatography media composed of a particulate substrate (such as polymer beads or other particulate substrates) that is coated with HA. Also provided is a chromatography media, (optionally contained within a column) comprising a HA-coated substrate (e.g., a plurality of beads or another particulate substrate that is coated with HA). The beads or particulate substrate can be a porous or non-porous substrate.
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
C01B 25/32 - Phosphates de magnésium, de calcium, de strontium ou de baryum
C04B 28/34 - Compositions pour mortiers, béton ou pierre artificielle, contenant des liants inorganiques ou contenant le produit de réaction d'un liant inorganique et d'un liant organique, p. ex. contenant des ciments de polycarboxylates contenant des liants phosphate froids
Provided herein is a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with hydroxyapatite ( HA ) Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Thus this disclosure provides a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with HA. Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Methods of preparing the HA-coated substrate are also provided.
Compositions and methods for fixing cells or nuclei or extracellular vesicles with dithiobismaleimidoethane (DTME) and subsequent reverse transcription in the cells or nuclei or extracellular vesicles are provided.
C40B 40/02 - Bibliothèques contenues ou présentées dans des micro-organismes, p. ex. des bactéries ou des cellules animalesBibliothèques contenues ou présentées dans des vecteurs, p. ex. des plasmidesBibliothèques contenant uniquement des micro-organismes ou des vecteurs
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
Compositions and methods for fixing cells or nuclei or extracellular vesicles with dithiobismaleimidoethane (DTME) and subsequent reverse transcription in the cells or nuclei or extracellular vesicles are provided.
An electronic component assembly having thermal pads with thermal vias coupling an image sensor and a camera board fab is provided for heat dissipation. The electronic component assembly can include: a circuit board having at least one thermal pad disposed on a top surface of the circuit board; and an image sensor disposed on the top surface of the circuit board, having at least one conductive pad disposed at at least one corner of the image sensor. The at least one thermal pad is coupled to the at least one conductive pad of the image sensor and the at least one thermal pad is formed with a plurality of first thermal vias penetrating the thermal pad and the circuit board for transfer of heat of the image sensor.
H04N 5/335 - Transformation d'informations lumineuses ou analogues en informations électriques utilisant des capteurs d'images à l'état solide [capteurs SSIS]
H04N 23/52 - Éléments optimisant le fonctionnement du capteur d'images, p. ex. pour la protection contre les interférences électromagnétiques [EMI] ou la commande de la température par des éléments de transfert de chaleur ou de refroidissement
Methods and compositions for nucleotide sequencing are provided. In some embodiments, click chemistry is used to link barcoding oligonucleotides to DNA fragments comprising adapters introduced by a transposase.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/26 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase
C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
A probe cleaning system and method including multiple pressurized sources and a valve that are configured for cleaning a probe. The valve includes multiple inlet channels, an outlet channel, and a selector. Each inlet channel can be connected to a specified pressurized cleaning source of the multiple pressurized cleaning sources. The plurality of inlet channels are isolated from each other to avoid cross-contamination. The selector can be configured to open a specified inlet channel to pass one specified pressurized cleaning source to the outlet channel at a specified time. The probe can be coupled to the outlet channel of the valve to receive the specified pressurized cleaning source when the specified inlet channel of the valve is opened during a cleaning cycle.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
A61B 1/00 - Instruments pour procéder à l'examen médical de l'intérieur des cavités ou des conduits du corps par inspection visuelle ou photographique, p. ex. endoscopesDispositions pour l'éclairage dans ces instruments
A61B 1/12 - Instruments pour procéder à l'examen médical de l'intérieur des cavités ou des conduits du corps par inspection visuelle ou photographique, p. ex. endoscopesDispositions pour l'éclairage dans ces instruments avec système de refroidissement ou de rinçage
A61B 90/70 - Dispositifs de nettoyage spécialement adaptés aux instruments chirurgicaux
A61L 2/00 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet
A61L 2/16 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des substances chimiques
A61L 2/18 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des substances chimiques des substances liquides
A61L 2/20 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des substances chimiques des substances gazeuses, p. ex. des vapeurs
Methods and compositions for nucleotide sequencing are provided. In some embodiments, click chemistry is used to link barcoding oligonucleotides to DNA fragments comprising adapters introduced by a transposase.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
The present relates to chromatic focusing via filter thickness tuning. Chromatic focusing can be achieved via an imaging system that includes a sample holding plane, a light source that illuminates the sample on the plane, and an imager that captures an image of the sample on the plane. The imager includes a sensor, a lens, and a filter wheel defining a plurality of filter apertures including a first aperture containing a first filter passing light of a first color and having a first focal point shift, and a second aperture containing a second filter passing light of a second color and having a second focal point shift. The focal point shifts cause both light of the first color passing through the first filter and through the lens and light of the second color passing through the second filter and through the lens to focus on the sensor.
Systems, methods, and devices for imaging of stain-free fluorescence on western blot membranes with excitation by epi illumination with UV LEDs are disclosed herein. A method can include collecting and preparing a sample, separating the sample via gel electrophoresis in a gel block, transferring the separated sample from the gel block to an analysis block, generating an image of the sample with an imager, and evaluating the image of the sample. The imager can include a plane to receive and hold a block containing a sample and including a first side and a second side, a camera to image a sample on the plane and positioned above the first side of the plane, and an LED light source positioned above the first side of the plane. The LED light source can illuminate the sample on the plane via epi-illumination, and emits light having a wavelength in a range from approximately 325 nm to approximately 400 nm.
The present relates to chromatic focusing via filter thickness tuning. Chromatic focusing can be achieved via an imaging system that includes a sample holding plane, a light source that illuminates the sample on the plane, and an imager that captures an image of the sample on the plane. The imager includes a sensor, a lens, and a filter wheel defining a plurality of filter apertures including a first aperture containing a first filter passing light of a first color and having a first focal point shift, and a second aperture containing a second filter passing light of a second color and having a second focal point shift. The focal point shifts cause both light of the first color passing through the first filter and through the lens and light of the second color passing through the second filter and through the lens to focus on the sensor.
Systems, methods, and devices for imaging of stain-free fluorescence on western blot membranes with excitation by epi illumination with UV LEDs are disclosed herein. A method can include collecting and preparing a sample, separating the sample via gel electrophoresis in a gel block, transferring the separated sample from the gel block to an analysis block, generating an image of the sample with an imager, and evaluating the image of the sample. The imager can include a plane to receive and hold a block containing a sample and including a first side and a second side, a camera to image a sample on the plane and positioned above the first side of the plane, and an LED light source positioned above the first side of the plane. The LED light source can illuminate the sample on the plane via epi-illumination, and emits light having a wavelength in a range from approximately 325 nm to approximately 400 nm.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Educational kits comprised primarily of assays for non-medical purposes in scientific labs, chemical reagents for non-medical purposes in scientific labs, cell growth media for growing cells for non-medical purposes in scientific labs, microbes being cells for non-medical purposes in scientific labs, and also containing laboratory equipment in the nature of plastic consumables being petri dishes, inoculation loops, microtubes and culture tubes for scientific laboratory use; chemical reagents for scientific laboratory educational purposes; biochemical reagents for scientific laboratory educational purposes; microbial preparations for scientific laboratory use, namely microbial strains being cells for scientific and research use, namely, for generating signals for scientific educational purposes; microbial preparations for scientific laboratory use, namely microbial strains being cells for non-medical purposes in scientific labs, namely, for generating signals for scientific educational purposes; none of the foregoing consisting of computer hardware
Disclosed herein are a system and method for determining a liquid level in a container based on data extracted from a digital image of the container. An image capture apparatus captures an image of a container used to store liquid reagents used by analytical instruments to test patient samples. A controller receives the captured image and extracts an analysis region based on a container type and applies a gradient filter to the analysis region. A location of a minimum gradient value (i.e., a row index) is determined based on the air-to-liquid boundary of the liquid in the extracted analysis region. A volume module calculates the liquid level in the analysis region by dividing the row index by a length of a gradient column vector and compares the determined liquid level to a threshold analysis region fraction for the container type to determine whether to output an alert.
G06T 7/62 - Analyse des attributs géométriques de la superficie, du périmètre, du diamètre ou du volume
G06T 7/73 - Détermination de la position ou de l'orientation des objets ou des caméras utilisant des procédés basés sur les caractéristiques
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 40/40 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion d’équipement ou de dispositifs médicaux, p. ex. pour planifier la maintenance ou les mises à jour
Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
B01F 33/302 - Micromixeurs les matières à mélanger s'écoulant sous forme de gouttelettes
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C40B 30/04 - Procédés de criblage des bibliothèques en mesurant l'aptitude spécifique à se lier à une molécule cible, p. ex. liaison anticorps-antigène, liaison récepteur-ligand
C40B 40/00 - Bibliothèques en soi, p. ex. matrices, mélanges
C40B 40/04 - Bibliothèques comprenant uniquement des composés organiques
C40B 70/00 - Étiquettes ["tags"] ou marqueurs ["labels"] spécialement adaptés à la chimie combinatoire ou aux chimiothèques, p. ex. "tags" fluorescents ou codes-barres
G01N 33/532 - Production de composés immunochimiques marqués
83.
METHODS AND COMPOSITIONS FOR NUCLEIC ACID ANALYSIS
Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
B01J 41/14 - Composés macromoléculaires obtenus par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 41/07 - Procédés utilisant des échangeurs organiques sous forme faiblement basique
B01J 47/014 - Procédés d'échange d'ions en généralAppareillage à cet effet dans lesquels les propriétés d’adsorption de l’échangeur d’ions sont utilisées, p. ex. récupération de protéines ou de composés macromoléculaires
A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
G01N 15/1433 - Traitement du signal utilisant la reconnaissance d’image
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 15/01 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux spécialement adaptée aux cellules biologiques, p. ex. aux cellules sanguines
G01N 15/10 - Recherche de particules individuelles
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
86.
SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
A system and method, wherein the method includes: (a) receiving a fluid comprising a process reagent for an assay and nucleic acid material from a sample into a fluid pathway coupled to an array of wells, wherein the array of wells is defined within a substrate and each well in the array of wells extends vertically into a planar surface of the substrate; (b) distributing the fluid along the fluid pathway and into wells of the array of wells; and (c) distributing a liquid immiscible with the fluid comprising the process reagent and nucleic acid material along the fluid pathway upon positive pressure generation by a pump of a flow control system in communication with an inlet to the fluid pathway, thereby displacing said fluid along the fluid pathway and into wells of the array of wells and preventing contents of each well from transferring to adjacent wells.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
G01N 15/10 - Recherche de particules individuelles
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.
G01N 33/571 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus pour maladies vénériennes, p. ex. syphilis, gonorrhée, herpès
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
89.
SYNTHESIZING BARCODING SEQUENCES UTILIZING PHASE-SHIFT BLOCKS AND USES THEREOF
Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.
Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.
Provided is a heterohybridoma-based method of generating recombinant rabbit monoclonal antibodies, recombinant anti-IL-6 receptor-alpha (IL-6Rα) antibodies generated using such methods, and immune assay methods kits employing such antibodies.
C07K 16/24 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des cytokines, des lymphokines ou des interférons
Methods and kits for purifying protein-nanoparticle conjugates are provided. In some embodiments, a multimodal medium having a size exclusion mode and a capture mode is used to purify the protein-nanoparticle conjugates.
A61K 47/69 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament le conjugué étant caractérisé par sa forme physique ou sa forme galénique, p. ex. émulsion, particule, complexe d’inclusion, stent ou kit
B01D 15/34 - Séparation par sélection en fonction de la taille, p. ex. chromatographie d'exclusion de tailleFiltration sur gelPerméation
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
C07K 1/16 - ExtractionSéparationPurification par chromatographie
C07K 16/00 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux
In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.
The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
B01F 33/3011 - Micromixeurs utilisant des moyens spécifiques pour disposer les écoulements à mélanger, p. ex. des géométries ou des dispositions de canaux utilisant un courant de gainage d'un fluide entourant un courant central d'un autre fluide, p. ex. pour réduire la section transversale du courant central ou pour produire des gouttelettes à partir du courant central
B01F 35/221 - Commande ou régulation des paramètres de fonctionnement, p. ex. du niveau de matière dans le mélangeur, de la température ou de la pression
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B05B 1/02 - Buses, têtes de pulvérisation ou autres dispositifs de sortie, avec ou sans dispositifs auxiliaires tels que valves, moyens de chauffage agencés pour produire un jet, un pulvérisat ou tout autre écoulement de forme ou de nature particulière, p. ex. sous forme de gouttes individuelles
B05B 1/08 - Buses, têtes de pulvérisation ou autres dispositifs de sortie, avec ou sans dispositifs auxiliaires tels que valves, moyens de chauffage agencés pour produire un jet, un pulvérisat ou tout autre écoulement de forme ou de nature particulière, p. ex. sous forme de gouttes individuelles de nature pulsatoire, p. ex. débitant un liquide en quantités successives séparées
B05B 1/26 - Buses, têtes de pulvérisation ou autres dispositifs de sortie, avec ou sans dispositifs auxiliaires tels que valves, moyens de chauffage avec des moyens pour briser ou dévier mécaniquement le jet à sa sortie, p. ex. des déflecteurs fixesDispersion du liquide ou d'autre matériau fluide sortant à l'aide de jets d'impact
B05B 7/00 - Appareillages de pulvérisation pour débiter des liquides ou d'autres matériaux fluides provenant de plusieurs sources, p. ex. un liquide et de l'air, une poudre et un gaz
98.
Dynamic axial compression for preparative columns using external compression
A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
100.
METHODS AND COMPOSITIONS FOR TRACKING BARCODES IN PARTITIONS
Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.
B01J 8/08 - Procédés chimiques ou physiques en général, conduits en présence de fluides et de particules solidesAppareillage pour de tels procédés avec des particules mobiles
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C40B 20/04 - Identification des éléments d'une bibliothèque au moyen d'une étiquette, d'un marqueur ou d'un autre identificateur lisible ou détectable, p. ex. procédés de décodage
C40B 40/08 - Bibliothèques comprenant de l'ARN ou de l'ADN codant des protéines, p. ex. bibliothèques de gènes
