An illustrative method of performing data-dependent tandem mass spectrometry includes a mass spectrometer acquiring an MS1 mass spectrum of ions produced from a sample, determining, based on the MS1 mass spectrum, a set of observed precursor ions, determining expected fragment ions for the set of observed precursor ions, and acquiring one or more MS2 mass spectra for select ions observed in the MS1 mass spectrum, wherein the expected fragment ions are excluded from being selected for MS2 analysis.
An illustrative method comprises determining, based on a mass analysis performed by an electron multiplier-based mass analyzer on a first ion population produced from a sample, a mass spectrum comprising one or more peaks representing intensity as a function of mass-to-charge ratio (m/z) of the first ion population across a range of m/z values; determining, based on the mass spectrum, a total ion count of the first ion population and a peak ion count associated with a peak located at a particular m/z value; determining, based on the total ion count of the first ion population and the peak ion count, a total ion count of a second ion population produced from the sample and injected into an image current-based mass analyzer for mass analysis; and setting, based on the total ion count of the second ion population, a calibration parameter for the image current-based mass analyzer.
A method of operating an analytical instrument comprises ionising a sample to produce sample ions; (i) performing a first ion separation scan by separating sample ions according to a first physico-chemical property, and analysing the separated sample ions by performing one or more MS1 mass analysis scans; and (ii) performing a second ion separation scan by separating sample ions according to the first physico-chemical property, and analysing the separated sample ions by performing a plurality of MS2 mass analysis scans. Each MS2 scan of the plurality of MS2 mass analysis scans uses one MS2 isolation window of a plurality of MS2 isolation windows. The method further comprises analysing MS1 data acquired from the one or more MS1 mass analysis scan(s), and configuring the plurality of MS2 isolation windows based on the analysis of the MS1 data.
A system for charge detection mass spectrometry (CDMS) performs a process including subdividing an m/z range of interest into a plurality of m/z windows; determining an ion population control parameter for each m/z window; accumulating, in the ion store by one or more accumulation events, a population of ions derived from a sample; transferring the population of ions to a mass analyzer; and mass analyzing the population of ions to acquire a CDMS spectrum of the population of ions. Each accumulation event corresponds to a distinct m/z window of the plurality of m/z windows. The ion population control parameter for each m/z window regulates a quantity of ions accumulated in an ion store during an accumulation event. During each accumulation event, ions within the m/z window corresponding to the accumulation event are accumulated in the ion store based on the ion population control parameter for the corresponding m/z window.
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
A method of performing automatic ion control for mass spectrometry includes acquiring, by charge detection mass spectrometry, a mass spectrum comprising a plurality of peaks representing intensity as a function of mass-to-charge ratio (m/z) of a population of ions analyzed by a mass analyzer during an acquisition event. Based on the mass spectrum, a measured signal density of a selected m/z range of the mass spectrum is determined. An ion population control parameter for a subsequent acquisition event is set based on the measured signal density and a target signal density. The ion population control parameter regulates a population of ions analyzed by the mass analyzer during the subsequent acquisition event.
A vacuum system for a mass spectrometer includes a first vacuum region, a second vacuum region, a vacuum interlock fluidly connected to the vacuum chamber by a first valve, a first pump fluidly connected to the first and second vacuum regions, a second pump fluidly connected to the second vacuum region, and a second valve. The second valve includes a housing, a piston movable within the housing between an evacuation position and an operation position, a first channel connected to a third valve, a second channel connected to the vacuum interlock, a third channel connected to the first pump, and a fourth channel connected to the second vacuum region. In response to the third valve adjusting to a first position, the piston moves to the evacuation position. In response to the third valve adjusting to the second position, the piston moved to the opened position.
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
A vacuum system for a mass spectrometer includes a first vacuum region, a second vacuum region, a vacuum interlock connected to the first vacuum region by a first valve and the second vacuum region by a second valve, a first pump connected to the first and second vacuum regions, a second pump connected to the second vacuum region, a pressure sensor configured to determine the pressure within the second vacuum region, and a controller configured to adjust a position of the second valve in response to the pressure within the second vacuum region. The vacuum interlock is configured to receive a sample. The first pump is configured to decrease a pressure within the first vacuum region and exhaust air to the second vacuum region. The second pump is configured to decrease a pressure within the vacuum interlock. The controller is configured to prevent pressure fluctuations in the second vacuum region.
A system for gas chromatography includes an inlet configured to receive a sample by injection, a column having a stationary phase, a flow control system, and an injection monitoring system. The flow control system is configured to regulate, based on a flow control parameter, flow of a mobile phase through the inlet and the column. The injection monitoring system is configured to obtain flow control data representative of a measure of the flow control parameter over time during a time period encompassing an injection of the sample into the inlet; determine, based on the flow control data, that the injection was unsuccessful; and perform, based on the determination that the injection was unsuccessful, a mitigation operation to mitigate the unsuccessful injection of the sample.
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
B01D 53/30 - Commande par appareil d'analyse des gaz
Systems, methods, algorithms, and instructions for segmenting data processing workflows are provided. In a first aspect, a computer-implemented method for segmenting data processing workflows includes determining a configuration of an instrument system. The instrument system can include an analytical instrument coupled with an instrument PC (IPC). The IPC can be configured to receive raw data from the analytical instrument, to process the raw data, and to communicate with a client computing device coupled with the instrument system. The method can include segmenting a data process workflow based at least in part on the configuration, attributing at least a subset of constituent operations of the data process workflow to the client computing device or the IPC. The method can also include generating an updated configuration of the instrument system, generating an updated latency parameter using the updated configuration, and modifying the segmented data process workflow using the updated latency parameter.
Systems, methods, and cartridges taught herein improve chromatographic performance in electrospray systems that feature chromatographic columns having a conductive or semiconductive stationary phase by electrically connecting a fluid junction located upstream of the chromatographic column to a fluid union located downstream of the chromatographic column using an electrical conductor. The electrical conductor creates a voltage equipotential between a first end of the chromatographic column and a second end of the chromatographic column that neutralizes current flow through the chromatographic column. Accurate electrospray current measurements are enabled while post-column peak dispersion and repeatable retention time are achieved.
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
A method for reducing peak tailing and reactions when using a carrier gas other than helium for gas chromatography-mass spectrometry (GC-MS) includes separating compounds using a gas chromatography column using a carrier gas other than helium; providing the column output to an ionization chamber of a mass spectrometer; providing a scavenger gas to the ionization chamber of the mass spectrometer; ionizing the compounds in the presence of the carrier gas and the scavenger gas; analyzing the ionized compounds using the mass spectrometer.
Embodiments herein relate to neural network control of mass spectrometry processes. A system can comprise a memory that stores, and a processor that executes, computer executable components. The computer executable components can comprise an acquisition component that acquires data for a compound, the data defining a first mass spectrometry spectrum for the compound, an evaluation component that, based on the data, and employing a neural network that is trained on an input dataset comprising an acquisition metric, and employing an associated score that is associated with the acquisition metric, generates a recommendation to perform a mass spectrometry action for the compound, and an execution component that, based on the recommendation, directs execution of the mass spectrometry action at a mass spectrometer and obtaining a mass spectrum result.
G05B 13/02 - Systèmes de commande adaptatifs, c.-à-d. systèmes se réglant eux-mêmes automatiquement pour obtenir un rendement optimal suivant un critère prédéterminé électriques
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
An example system includes a set of multipole electrodes and an RF generator configured to selectively output a signal voltage at a first frequency or at a second frequency for driving the set of multipole electrodes. The system further includes a low-frequency transformer assembly and a high-frequency transformer assembly. The system further includes a switching circuit configured to connect the RF generator to: the low-frequency transformer assembly when the RF generator outputs the RF voltage at the first resonant frequency, the low-frequency transformer assembly configured to supply a first voltage based on the RF voltage to an output coupled to the set of multipole electrodes, or the high-frequency transformer assembly when the RF generator outputs the RF voltage at the second resonant frequency, the high-frequency transformer assembly configured to supply a second voltage based on the RF voltage to the output coupled to the set of multipole electrodes.
A system may control a mass spectrometer to acquire, during a plurality of acquisitions constituting an acquisition cycle, a set of mass spectra of product ions derived from precursor ions isolated based on a parallel isolation window successively positioned throughout a precursor mass-to-charge ratio (m/z) range. The precursor m/z range is divided into a plurality of isolation window units. The parallel isolation window includes, for each acquisition of the acquisition cycle, a set of isolation sub-windows corresponding to a distinct set of isolation window units of the precursor m/z range. At least two adjacent isolation sub-windows of the parallel isolation window are non-contiguous. Each isolation window unit of the precursor m/z range is analyzed at least twice during the acquisition cycle. A mass spectrum for the precursor m/z range may be generated based on the set of mass spectra acquired during the acquisition cycle.
A method of manufacturing an ion optical device includes printing the ion optical device in a printing direction from a first end to a second end. The ion optical device includes a first end support at the first end, a first set of rods extending from the first end support and formed with a first set of rings, a second set of rods extending from the first end support and formed with a second set of rings, and a second end support at the second end to which the first and second sets of rods extend. Cutting the first and second end supports away from the ion optical device defines a first electrical circuit through the first set of rods and the first set of rings and a second electrical circuit through the second set of rods and the second set of rings that are electrically isolated from each other.
It is proposed to improve transmission of ions along the curved path of an ion guide by changing the separation between adjacent multipole rods in the plane of curvature. As the separation increases along the length of the device, in some embodiments, the ion confinement in the plane of curvature decreases. At the same time, in some embodiments, the external field penetration from the electrodes outside of the main ion guide increases, which can be used to create additional DC field gradients to facilitate ion confinement and motion through the ion guide.
A pulse control system may obtain, for each ion pulse included in a plurality of ion pulses, a signal acquired by a mass analyzer for ions ejected from a distinct set of channels of an ion mobility separator having a plurality of channels and determine, based on the signals for the plurality of ion pulses and a Hadamard algorithm, a signal associated with the ions ejected from a channel of the ion mobility separator.
A deuterium gas generator system. The deuterium gas generator system comprises an electrolysis cell and a palladium alloy purifier membrane having a surface area of not more than 10 square centimeters. The present disclosure relates to uses and methods of employing deuterium gas as carrier gas for gas chromatography and mas spectrometry applications.
C01B 5/02 - Eau lourdePréparation par réaction chimique des isotopes de l'hydrogène ou de leurs composés, p. ex. 4ND3 + 7O2→ 4NO2 + 6D2O, 2D2 + O2→ 2D2O
B01D 53/32 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par effets électriques autres que ceux prévus au groupe
B01D 59/40 - Séparation par méthodes électrochimiques par électrolyse
19.
DEUTERIUM GAS GENERATOR AND DEVICES FOR CONSERVATION THEREOF
An analytical instrument. The analytical instrument includes of one or both of an ion trap or a collision cell. One or both of the ion trap or collision cell is filled with deuterium gas.
An analytical instrument. The analytical instrument includes of one or both of an ion trap or a collision cell. One or both of the ion trap or collision cell is filled with deuterium gas.
A deuterium gas generator system. The deuterium gas generator system comprises an electrolysis cell and a palladium alloy purifier membrane having a surface area of not more than 10 square centimeters.
B01D 15/16 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement du fluide vecteur
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
Real-time search (RTS) for mass spectrometry is described. In one aspect, a mass spectrometer can identify a candidate ion for a product ion spectrum by searching a mass spectral database. While executing the search of the mass spectral database, the elapsed search time can be monitored. If the elapsed search time reaches a maximum search time value prior to an identification of a matching candidate ion, then the mass spectrometer can terminate the search. If the elapsed search time of the identification of the candidate ion is completed before reaching a maximum value, then the mass spectrometer can perform further actions.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/42 - Spectromètres à stabilité de trajectoire, p. ex. monopôles, quadripôles, multipôles, farvitrons
23.
SYSTEMS AND METHODS OF ION POPULATION REGULATION IN MASS SPECTROMETRY
A computer-implemented method of training a machine learning model comprises: accessing an elution profile comprising a plurality of detection points representing intensity of ions derived from components eluting from a chromatography column and detected by a mass analyzer as a function of time; generating, based on the elution profile, training data comprising a plurality of training examples, a training example of the plurality of training examples comprising a set of detection points and a target next detection point, the target next detection point comprising a detection point of the plurality of detection points following the set of detection points; and training, using the training data, the machine learning model to determine a predicted next detection point, the predicted next detection point following the set of detection points.
A method of operating an ion optical component comprising a series of electrodes between first and second ends comprises: applying a set of RF voltage waveforms to electrodes of the series that generate a plurality of moving pseudopotential wells that exert forces on ions within the ion optic that urge the ions to migrate from the first end to the second end of the ion optic; and applying, simultaneously with the application of the set of RF voltage waveforms, a set of DC electrical potentials to electrodes of the series that generate a DC field that exerts a force on the ions within the ion optic that urges the ions to migrate from the second end to the first end, whereby there is caused one or more of spatial separation, differential migration or filtering of ions within the ion optical component in accordance with their respective mass-to-charge (m/z) ratios.
Systems or techniques are provided for facilitating idle-triggered diagnostics for scientific instruments. In various embodiments, a scientific instrument can comprise a mass spectrometer coupled to a chromatograph. In various aspects, the scientific instrument can determine, via a temperature sensor of the chromatograph or via a clock of the mass spectrometer, whether the scientific instrument is in an idle-time period. In various instances, the scientific instrument can, in response to a determination that the scientific instrument is in the idle-time period, apply one or more electronic control signals to the mass spectrometer or to the chromatograph, measure one or more resultant ion spectra via an ion detector of the mass spectrometer, and determine whether the mass spectrometer or the chromatograph is operating correctly based on the one or more resultant ion spectra.
Systems or techniques are provided for facilitating intelligent maintenance for scientific instruments. In various embodiments, a scientific instrument can comprise a chromatograph-equipped mass spectrometer. In various aspects, the scientific instrument can determine, based on an electronic counter or readback sensor associated with the chromatograph-equipped mass spectrometer failing to satisfy a threshold, whether performance of a maintenance task on the chromatograph-equipped mass spectrometer is warranted. In various instances, the scientific instrument can schedule, in response to a determination that the performance of the maintenance task is warranted, a time or date for the performance of the maintenance task, wherein the time or date can be predicted by a machine learning model based on an operational history of the chromatograph-equipped mass spectrometer. In various cases, the scientific instrument can prepare for the performance of the maintenance task, by adjusting, prior to the time or date, actuatable hardware of the chromatograph-equipped mass spectrometer.
Gas is flowed through a gas chromatography column at a first flow rate to cause a first change in pressure defining a first pressure differential. The first pressure differential and/or a first time duration for the first change in pressure is measured. At least one closed exhaust path is opened and a second flow rate through each of the at least one open exhaust paths is set. Gas is flowed through the column at a third flow rate and each of the at least one open exhaust paths at the second flow rate, causing a second change in pressure defining a second pressure differential. The second pressure differential and/or a second time duration for the second change in pressure is measured. A leak is determined based on one or more of measured first pressure differential and/or first time duration and measured second pressure differential and/or second time duration.
G01N 30/32 - Contrôle des paramètres physiques du fluide vecteur de la pression ou de la vitesse
G01M 3/26 - Examen de l'étanchéité des structures ou ouvrages vis-à-vis d'un fluide par utilisation d'un fluide ou en faisant le vide par mesure du taux de perte ou de gain d'un fluide, p. ex. avec des dispositifs réagissant à la pression, avec des indicateurs de débit
A mass spectrometer ion transport system comprises: (a) an ion transfer tube for receiving ions from an atmospheric pressure ionization ion source and comprising an ion outlet end; (b) an apparatus comprising: a first electrode section comprising a first ion transport volume and configured to receive the ions from the ion outlet end of the ion transfer tube; an ion funnel comprising an ion inlet aperture configured to receive the ions from the first electrode section, a second ion transport volume, and an ion outlet aperture configured to transfer the ions from the second ion transport volume to a mass analyzer, and (c) an auxiliary tube for delivering an auxiliary flow of gas into the first electrode section; wherein the ion funnel ion inlet aperture is offset from a linear axis defined between the ion transfer tube ion outlet end and the ion funnel ion outlet aperture.
Systems and methods taught herein generate a non-uniform magnetic field in the ionization region of an ion source to improve robustness in electrical and chemical ionization processes, particularly negative chemical ionization (CI) processes. The non-uniform magnetic field within the ionization volume spatially separates electrons and anions such that anions primarily pass through an ion exit aperture in the ionization chamber while electrons are directed to strike side walls or end walls of the ionization chamber away from the ion exit aperture. As a result, greater numbers of ions exit from the ion source towards a mass analyzer. Systems and methods taught herein also increase the longevity of instrumentation by avoiding damage that can be caused by electrons striking surfaces around apertures.
A method of processing mass spectral data is provided. The mass spectral data includes a plurality of MS1 mass spectra and a plurality of MSN mass spectra each having a respective associated retention time. A group of features is detected in the plurality of MS1 mass spectra, each feature of the group having a respective mass, and the features of the group having corresponding retention times. The method includes, for each of one or more features of the group: submitting a corresponding MSN mass spectrum to a mass spectral search engine in order to obtain an identification result for that feature, and determining a candidate ion type for the feature based on a mass difference between the mass associated with the feature and an expected mass from the identification result. The method also includes identifying one or more compounds based on the group of features and the candidate ion type(s).
A scientific instrument support apparatus includes memory hardware configured to store instructions and processing hardware configured to execute the instructions. The instructions include processing metadata to identify extended attributes present in the metadata, loading one or more semantic models based on media type annotations in the metadata, querying the loaded one or more semantic models for corresponding extended attribute properties that match extended attribute properties in the metadata, and, in response to finding corresponding extended attribute properties in a selected semantic model, matching each extended attribute property in the metadata to one of the corresponding data properties in the selected semantic model, aligning each corresponding data property to a node on an output graph, generating relationships between nodes on the output graph based on object properties, testing relationships between nodes, removing erroneous relationships from the output graph, and transforming a graphical user interface to display the output graph.
Methods and systems for ion trap type resonant collision induced dissociation (CID) on labile compounds to drive selective dissociation along desired dissociation pathways are disclosed. Specifically, the present disclosure includes, systems, methods, and computer readable media (CRM) configured to introduce ions of at least one labile compound with multiple dissociation pathways into a trapping area of a dissociation cell. The dissociation cell is configured to generate a substantially quadrupolar radio frequency electrostatic confinement potential in the ion trapping region. The kinetic energy of selected precursor ions is increased while a selective dissociation parameter is maintained to cause the precursor ions to selectively dissociate primarily to first-generation products without being ejected from the trapping area. The selective dissociation parameter is determined in part by the Mathieu q value and controls the maximum kinetic energy or velocity of the ions.
A position control system may acquire a set of mass spectra by directing an automated positioning system to sequentially position an ionization emitter at a plurality of positions relative to an inlet of a mass spectrometer and directing the mass spectrometer to acquire, while the ionization emitter is positioned at each position of the plurality of positions, a mass spectrum of ions introduced into the inlet. The ions introduced into the inlet include ions emitted from the ionization emitter. The position control system may generate, based on the set of mass spectra, an ion intensity map representing intensity of ions introduced into the inlet of the mass spectrometer as a function of position of the ionization emitter. Based on the ion intensity map, the position control system may identify an optimum position for the ionization emitter.
THERMO ELECTRON MANUFACTURING LIMITED (Royaume‑Uni)
DIONEX CORPORATION (USA)
Inventeur(s)
Hadfield, Maximillian Spencer
Schroeder, Tara L.
Lin, Linda Xueying
Rehman, Misbah
Cao, Yu
Cardasis, Helene L.
Zhou, Yu
Kenny, Patrick
Yuen, Waiming
Schauer, Kevin
Abrégé
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a support system is provided for a scientific instrument. The support system is configured to acquire, for each of a plurality of scientific instrument groups, asset information associated with the scientific instrument group. The scientific instrument group includes at least one scientific instrument. The support system is also configured to acquire, for each of the plurality of scientific instrument groups, reservation information associated with the scientific instrument group. The support system is also configured to generate, for each of the plurality of scientific instrument groups, a status card including status information associated with the scientific instrument group. The status information is at least based on the asset information and the reservation information. The support system is configured to provide a first user interface for displaying the status cards.
A method for analyzing mRNA capping including hybridizing mRNA to a probe, the probe including a 3' ribonuclease segment, an interior deoxyribonuclease segment, and a 5' ribonuclease segment; cleaving the mRNA with a RNA:DNA specific ribonuclease; and analyzing the cleaved mRNA oligomer using liquid chromatography-mass spectrometry (LC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS).
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
THERMO ELECTRON MANUFACTURING LIMITED (Royaume‑Uni)
THERMO FINNIGAN LLC (USA)
DIONEX CORPORATION (USA)
Inventeur(s)
Hadfield, Maximillian
Schroeder, Tara
Lin, Linda
Rehman, Misbah
Cao, Yu
Cardasis, Helene
Zhou, Yu
Yuen, Waiming
Kenny, Patrick
Schauer, Kevin
Abrégé
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a support system is provided for a scientific instrument. The support system is configured to acquire, for each of a plurality of scientific instrument groups, asset information associated with the scientific instrument group. The scientific instrument group includes at least one scientific instrument. The support system is also configured to acquire, for each of the plurality of scientific instrument groups, reservation information associated with the scientific instrument group. The support system is also configured to generate, for each of the plurality of scientific instrument groups, a status card including status information associated with the scientific instrument group. The status information is at least based on the asset information and the reservation information. The support system is configured to provide a first user interface for displaying the status cards.
A position control system may obtain image data representative of one or more images that depict an inlet of a mass spectrometer and an emitter positioned near the inlet and adjust, based on the image data, a position of the emitter relative to the inlet to an optimum position that is at or near a reference position relative to the inlet.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
G06T 7/73 - Détermination de la position ou de l'orientation des objets ou des caméras utilisant des procédés basés sur les caractéristiques
G06V 10/12 - Détails des dispositions d’acquisitionLeurs détails structurels
G06V 20/52 - Activités de surveillance ou de suivi, p. ex. pour la reconnaissance d’objets suspects
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
A system for collecting multiple compensation voltage fractions includes a field asymmetric-waveform ion-mobility spectrometry (FAIMS) device which receives ions generated from a sample, such as a biological sample. A mass spectrometer injection gate is configured to inject, when in an open state, ions transmitted by the FAIMS device into a mass analyzer of the mass spectrometer. A controller circuit is configured to open the injection gate and apply a dynamic compensation voltage (CV) to an electrode of the FAIMS device while the injection gate is open. The dynamic CV can have a waveform in the shape of a ramp, triangle, wavelet, sinusoid, or another shape. The dynamic CV includes CVs associated with multiple adjacent CV fractions, and the FAIMS device is configured to transmit the multiple adjacent CV fractions into the mass analyzer via the open injection gate to be scanned together by the mass analyzer in a single experiment.
A computing device for mass spectrometry generates an acquisition schedule that schedules acquisition, by a mass spectrometer, of a set of mass spectra for each target analyte included in a plurality of target analytes included in a sample as the plurality of target analytes elute from a separation system. The acquisition schedule specifies a dynamic acquisition cycle period that varies over time. The computing device further directs the mass spectrometer to acquire the mass spectra in accordance with the acquisition schedule. In some examples, an acquisition schedule is generated by identifying an analyte group corresponding to each analyte included in a list of analytes estimated to be present in the sample and selecting, from the list of analytes, the set of target analytes based on selection criteria and the analyte group corresponding to each respective analyte included in the list of analytes.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
B01D 15/08 - Adsorption sélective, p. ex. chromatographie
Disclosed herein are instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, an instrument support apparatus may include: first logic to first logic to receive a configuration comprising identifying data related to an instrument that provides telemetry data, and a plurality of settings for the instrument regarding a set of the telemetry data provided by the instrument; second logic to configure a control layer of the instrument to provide the set of the telemetry data according to the plurality of settings; third logic to receive, from the control layer, telemetry data from the set of the telemetry data; fourth logic to format the received telemetry data; and fifth logic to provide the formatted telemetry data for further processing.
H04Q 9/00 - Dispositions dans les systèmes de commande à distance ou de télémétrie pour appeler sélectivement une sous-station à partir d'une station principale, sous-station dans laquelle un appareil recherché est choisi pour appliquer un signal de commande ou pour obtenir des valeurs mesurées
A method of determining a calibration for an analytical instrument comprises ionising a calibrant to produce calibrant ions, performing a sequence of ion separation scans, performing a plurality of mass analysis scans by performing one or more mass analysis scan(s) during each ion separation scan of the sequence, and using data obtained from the plurality of mass analysis scans to determine a calibration for the analytical instrument. Each ion separation scan has a duration TIMS, and each mass analysis scan has a duration TMA. Each ion separation scan has a respective start time ti0, and each mass analysis scan has a respective start time tijMA defined relative to the start time ti0 of the ion separation scan during which the mass analysis scan is performed. The start times tijMA of the plurality of mass analysis scans include start times separated by a delay time Δt, wherein Δt
Apparatus and methods for charge detection mass spectrometry cause an ion of interest to undergo harmonic oscillatory movement in the trapping field of an electrostatic trap, such that an image current detector generates a time-varying signal representative of the ion's oscillatory movement. This time-varying signal (transient) is processed (e.g., via a Fourier transform) to derive the ion's frequency and consequently determine the ion's mass-to-charge ratio (m/z). Ion charge is determined by construction of a Selective Temporal Overview of Resonant Ion (STORI) plot, which tracks the temporal evolution of signals attributable to the ion of interest, and where the slope of the STORI plot is related to the charge. The STORI plot may also be employed to identify ion decay events during transient acquisition and/or the presence of multiple ions of the same mass or non-resolvable ions.
Disclosed herein are instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, an instrument support apparatus may include: first logic to first logic to receive a configuration comprising identifying data related to an instrument that provides telemetry data, and a plurality of settings for the instrument regarding a set of the telemetry data provided by the instrument; second logic to configure a control layer of the instrument to provide the set of the telemetry data according to the plurality of settings; third logic to receive, from the control layer, telemetry data from the set of the telemetry data; fourth logic to format the received telemetry data; and fifth logic to provide the formatted telemetry data for further processing.
Disclosed herein are systems, methods, computing devices, and computer-readable media. For example, in some embodiments, a method for extract, transform and load (ETL) processing executed by a processing device, the method comprising: receiving, from a data catalog, field mapping between application data and a target schema; receiving from an ETL processing queue, signal comprising metadata and indicating that a record or a data file related to the application data is ready for processing; determining source data by processing the metadata to identify a location of the record or the data file and retrieving the record or the data file from the identified location; and providing source data to a table defined according to the target schema.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus may include: first logic to receive, from a mass spectrometer, injections data for each of a plurality of injections associated with a sample; second logic to determine peak data for each of the plurality of injections by executing, in parallel on a node, a peak detection algorithm for each of the plurality of injections, third logic to collate the peak data for each of the plurality of injections; and fourth logic to provide the collated peak data for further processing.
A process for charge detection mass spectrometry includes acquiring a time-varying signal representative of a current induced on a detector by oscillatory motion of an ion within a trapping region; processing the time-varying signal to derive a frequency of the oscillatory motion; generating, based on the amplitude of the time-varying signal and the derived frequency of the oscillatory motion, Selective Temporal Overview of Resonant Ion (STORI) data representing STORIreal values versus time and STORIimag values versus time; regenerating the STORI data based on a variation of the frequency of the oscillatory motion over time; and determining a charge state of the ion based on the regenerated STORI data.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus may include: first logic to receive, from a mass spectrometer, injections data for each of a plurality of injections associated with a sample; second logic to determine peak data for each of the plurality of injections by executing, in parallel on a node, a peak detection algorithm for each of the plurality of injections, third logic to collate the peak data for each of the plurality of injections; and fourth logic to provide the collated peak data for further processing.
realimagimag values versus time; regenerating the STORI data based on a variation of the frequency of the oscillatory motion over time; and determining a charge state of the ion based on the regenerated STORI data.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. In one example, an automated method performed via a computing device for providing scientific instrument support comprises: transferring portions of a data structure acquired with detectors of a scientific instrument to a client device, the data structure being stored in a data storage connected via a network to the computing device; identifying parts of the data structure based on a data-access pattern in a sequence of data requests; downloading the parts from the data storage to the storage device locally connected to the computing device; and switching a data transfer path for the client device from being end-connected to the data storage to being end-connected to the storage device when a requested portion of the data structure is in the parts downloaded to the storage device via the computing device.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. In one example, an automated method performed via a computing device for providing scientific instrument support comprises: transferring portions of a data structure acquired with detectors of a scientific instrument to a client device, the data structure being stored in a data storage connected via a network to the computing device; identifying parts of the data structure based on a data-access pattern in a sequence of data requests; downloading the parts from the data storage to the storage device locally connected to the computing device; and switching a data transfer path for the client device from being end-connected to the data storage to being end-connected to the storage device when a requested portion of the data structure is in the parts downloaded to the storage device via the computing device.
A mass spectrometer support apparatus includes a peak shape logic to determine one or more peak shapes using a calibration mass spectrum and known peak locations; and a tuning logic to adjust instrument parameters to achieve a selected peak width. A method for tuning a quadrupole-based mass spectrometer includes determining one or more peak shapes using a calibration mass spectrum and known peak locations; and adjusting instrument parameters to achieve a selected peak width.
A data dependent acquisition method of mass spectrometry using a dual analyser mass spectrometer for analysing a sample comprises the steps of: ionising the sample to produce a plurality of precursor ions; performing, by a first mass analyser, an MS1 scan of the precursor ions from the sample and identifying precursor ions of interest; selecting and fragmenting precursor ions of interest to produce first fragmented ions, and performing, by a second mass analyser, MS2 scans of the first fragmented ions; selecting and fragmenting further precursor ions of interest to produce second fragmented ions, and performing, by the first mass analyser, MS2 scans of the second fragmented ions, wherein the second mass analyser operates in an opposite polarity to the first mass analyser so as to generate MS2 scans of fragmented ions having an opposite polarity to the fragmented ions of the MS2 scans generated by the first mass analyser.
Aspects of systems, methods, algorithms, and non-transitory media storing computer-readable instructions for segmenting data processing workflows are provided. In a first aspect, a computer-implemented method for segmenting data processing workflows includes determining a configuration of an instrument system. The instrument system can include an analytical instrument coupled with an instrument PC (IPC). The IPC can be configured to receive raw data from the analytical instrument, to process the raw data, and to communicate with a client computing device coupled with the instrument system. The method can also include segmenting a data process workflow based at least in part on the configuration, attributing at least a subset of constituent operations of the data process workflow to the client computing device or the IPC.
Aspects of systems, methods, algorithms, and non-transitory media storing computer-readable instructions for segmenting data processing workflows are provided. In a first aspect, a computer-implemented method for segmenting data processing workflows includes determining a configuration of an instrument system. The instrument system can include an analytical instrument coupled with an instrument PC (IPC). The IPC can be configured to receive raw data from the analytical instrument, to process the raw data, and to communicate with a client computing device coupled with the instrument system. The method can also include segmenting a data process workflow based at least in part on the configuration, attributing at least a subset of constituent operations of the data process workflow to the client computing device or the IPC.
G06Q 10/0631 - Planification, affectation, distribution ou ordonnancement de ressources d’entreprises ou d’organisations
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
G16B 40/10 - Traitement du signal, p. ex. de spectrométrie de masse ou de réaction en chaîne par polymérase
G06F 9/30 - Dispositions pour exécuter des instructions machines, p. ex. décodage d'instructions
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
55.
TRAINED NEURAL NETWORK MODEL TO DETERMINE CARTRIDGE AND SYSTEM HEALTH
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a chromatography instrument support apparatus may include: first logic to receive, from an imaging device, image data regarding chromatography instrumentation; second logic to determine a state of the chromatography instrumentation by processing the imaging device through a machine-learning computational model; and third logic to display the state of the chromatography instrumentation.
G06V 10/82 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant les réseaux neuronaux
56.
INSTRUMENT OPTIMIZATION USING ANALYTE BASED MASS SPECTROMETER AND ALGORITHM PARAMETERS
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, an apparatus, includes a sample introduction device and mass spectrometer. The mass spectrometer includes first logic to receive a sample from a queue, the sample comprising an analyte; second logic to determine instrument methods and data analysis parameters based on a nature of the analyte; and third logic to process the sample by applying the instrument methods and data analysis parameters.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
A method for separation of analyzing oligonucleotides or nucleic acid biopolymers includes loading a sample containing a plurality of oligonucleotide species onto a column; flowing a mobile phase consisting of a combination of a first solution and a second solution through the column to elute the plurality of oligonucleotide species; and analyzing at least one of the first oligonucleotide species and the second oligonucleotide species using a mass spectrometer. The plurality of oligonucleotide species includes a first oligonucleotide species and a second oligonucleotide species. The first solution includes an ion pairing reagent. The ion pairing reagent includes a primary amine or a secondary amine. The proportion of the first solution and the second solution in the mobile phase is varied over time to separate the first oligonucleotide species from the second oligonucleotide species.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
Systems and methods under the present disclosure can provide communication between instruments or resources at multiple locations. One example is a system of interconnection for various types of assets at multiple laboratories in different locations. Assets, devices, resources, or services at a location can multicast a beacon identifying itself. Verification of the asset, etc., can be done by communicating with a URI associated with that asset. Authentication of assets can be token-based to enable communication. Proxy servers at all or some of the locations can manage authentication and communication between locations and between assets. Tunnels can be implemented between different locations to help ensure secure communication.
An ion mobility analyser is disclosed having a gas flow directed along the ion travel axis and a set of electrodes to which DC voltages are applied to establish a DC field. The opposing forces of the gas flow and DC field cause ions to be trapped within a separation region in axial regions determined by their ion mobilities. A gas recirculator, having inlet and outlet ends respectively located downstream and upstream of the separation region, supplies at least fifty percent of the gas flow within the separation region, thereby reducing vacuum pumping requirements.
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/06 - Dispositifs électronoptiques ou ionoptiques
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a mass spectrometry instrument support apparatus may include: first logic to receive a plurality of sample data from a queue; second logic to generate, based on the sample data, a gel-lane plot comprising a stacked column of gel spots where the y-axis represents is the mass, and the x-axis is discretized into a single sample or run; third logic to regularize the y-axis to the sample data by employing a clustering technique (e.g., including a high-resolution clustering algorithm and/or a low-resolution version) to mass cluster the sample data; and fourth logic to provide the gel-lane plot for display showing the deconvoluted mass spectra results for samples.
A method of performing mass spectrometry includes obtaining, based on a series of mass spectra acquired over time with a first sampling rate as analytes elute from a separation system during an experiment, a first mass chromatogram dataset. The first mass chromatogram dataset represents a detected intensity of ions derived from the analytes and having a selected m/z as a function of time over a time period. The method further includes generating, based on the first mass chromatogram dataset and an upsampling model trained to upsample mass chromatogram data, a second mass chromatogram dataset representing an estimated intensity of the ions as a function of time over the time period. The second mass chromatogram dataset has a second sampling rate that is greater than the first sampling rate.
A method for quantifying mRNA capping efficiency includes combining a sample, an enzyme mixture, and an isotopic standard solution in a buffer solution to create an incubation mixture, the enzyme mixture including a non-specific, single-stranded nuclease and an acid phosphatase and the isotopic standard including isotopically labeled m7G and isotopically labeled 2′-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of a capping efficiency and a 2-O-methyltransferase efficiency.
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
A mass spectrometer includes a vacuum chamber, a pump for maintaining a vacuum chamber at an operating vacuum pressure, an ion source, a first mass filter configured to select precursor ions, a collision/reaction cell pressurized with a collision or reaction gas and configured to generate a plurality of product ions from the precursor ions by colliding or reacting the precursor ions with one or more gas particles, and a second mass filter configured to select target ions from the product ions. Also provided are entrance and exit lenses between the collision/reaction cell and the first and second mass filters, respectively, each of which include axially-spaced ion lenses and evacuation chambers between adjacent ion lenses. A plenum fluidly connects the evacuation chambers to the pump inlet to facilitate evacuation of collision or reaction gas escaping the collision/reaction cell to the pump away from the first and second mass filters.
A mass spectrometry method comprises: identifying groups of charge state distributions (CSDs) within deconvoluted mass spectrometric data, wherein CSDs of each group comprise at least one common mass spectral peak that is assigned a different respective charge state within each CSD of each group; assigning, within each group, a respective weighting factor to each CSD; calculating, within each group and using the weighting factors, a score-weighted average molecular weight for each compound CSD; locating, within each group, a single target CSD that that corresponds to a molecular weight that is closest to the calculated average; for each common peak of each identified group, summing the intensities of the respective common assigned mass spectral peak across the group and assigning the summed intensity to the single target CSD of the group; discarding all CSDs other than the target CSDs; and calculating an abundance of each component compound using the summed intensities.
G06F 18/2415 - Techniques de classification relatives au modèle de classification, p. ex. approches paramétriques ou non paramétriques basées sur des modèles paramétriques ou probabilistes, p. ex. basées sur un rapport de vraisemblance ou un taux de faux positifs par rapport à un taux de faux négatifs
Real-time search (RTS) for mass spectrometry is described. In one aspect, a mass spectrometer can identify a candidate peptide for a product ion spectrum by searching a mass spectral database. While executing the search of the mass spectral database, the elapsed search time can be monitored. If the elapsed search time of the identification of the candidate peptide is completed before reaching a maximum value, then the mass spectrometer can perform further actions.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/42 - Spectromètres à stabilité de trajectoire, p. ex. monopôles, quadripôles, multipôles, farvitrons
66.
METHODS FOR RESOLVING CHARGE-STATE AMBIGUITIES IN HIGH AND ULTRA-HIGH MASS RANGE MASS SPECTRA
A mass spectrometry method comprises: identifying groups of charge state distributions (CSDs) within deconvoluted mass spectrometric data, wherein CSDs of each group comprise at least one common mass spectral peak that is assigned a different respective charge state within each CSD of each group; assigning, within each group, a respective weighting factor to each CSD; calculating, within each group and using the weighting factors, a score-weighted average molecular weight for each compound CSD; locating, within each group, a single target CSD that that corresponds to a molecular weight that is closest to the calculated average; for each common peak of each identified group, summing the intensities of the respective common assigned mass spectral peak across the group and assigning the summed intensity to the single target CSD of the group; discarding all CSDs other than the target CSDs; and calculating an abundance of each component compound using the summed intensities.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
Disclosed herein are systems and methods for sorting ions including a group of multipole electrodes configured to form an ion trap, and an ion guide adjacent to, and operably coupled to the group of multipole electrodes. Using a radio frequency (RF) or Direct Current (CD) power supply device the system can apply an RF voltage to the group of multipole electrodes thereby creating a pseudo-potential barrier. A DC gradient voltage may then be applied creating an axial field in opposition to the pseudo-potential barrier. As the DC voltage is raised and/or the RF voltage is lowered, one or more ions will be eluted through the barrier.
Systems, devices, and methods for in-source ion separation are provided. An ion separator includes an ion transfer conduit fluidically upstream of and coupled with one or more components of an analytical instrument. The ion separator includes a gas conduit, fluidically upstream of and coupled with the ion transfer conduit, the gas conduit defining an internal volume. The ion separator also includes electronic circuitry defining an active surface exposed to the internal volume, the electronic circuitry being configured to energize the active surface. Embodiments of the present disclosure provide improved analysis of material samples based at least in part on in-source separation of relatively light ions from relatively heavy ions entrained in a gas flow.
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
69.
DATA STORAGE FOR SCALABLE PROCESSING OF LARGE FILES GENERATED BY SCIENTIFIC INSTRUMENTS
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a support apparatus for a scientific instrument comprises first, second, and third logics. The first logic is configured to acquire a first data file via detectors of the instrument. The second logic is configured to convert the first data file into a plurality of second data files stored in an object storage. Each of the second files is named using a suitable file naming convention. The third logic is configured to process a request for a data portion of the first data file and to provide the data portion by accessing one or more of a first memory cache, a second memory cache, and the object storage to obtain a corresponding portion of a corresponding one of the second data files identified based on the file naming convention.
G06F 12/0802 - Adressage d’un niveau de mémoire dans lequel l’accès aux données ou aux blocs de données désirés nécessite des moyens d’adressage associatif, p. ex. mémoires cache
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a support apparatus for a scientific instrument comprises first, second, and third logics. The first logic is configured to acquire a first data file via detectors of the instrument. The second logic is configured to convert the first data file into a plurality of second data files stored in an object storage. Each of the second files is named using a suitable file naming convention. The third logic is configured to process a request for a data portion of the first data file and to provide the data portion by accessing one or more of a first memory cache, a second memory cache, and the object storage to obtain a corresponding portion of a corresponding one of the second data files identified based on the file naming convention.
The present invention relates to hydrogen generation for use in gas chromatography applications. In particular, the present invention relates to hydrogen generators producing less than 100 standard cubic centimeters per minute of hydrogen and the use of said hydrogen generators in gas chromatography systems and applications.
C25B 15/08 - Alimentation ou vidange des réactifs ou des électrolytesRégénération des électrolytes
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
B01D 53/22 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par diffusion
Disclosed herein are scientific instrument support systems, related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus includes sample analysis logic to cause a sample to be injected into a gas chromatograph and to obtain a mass spectrum of the sample using a mass spectrometer coupled to the output of the gas chromatograph, the mass spectrometer utilizing a mass calibration or a lock mass correction while obtaining the mass spectrum of the sample; calibration gas logic to control a supply of a calibration gas to the mass spectrometer during the void time of the gas chromatograph; and lock mass calibration logic to obtain a mass spectrum of the calibration gas using the mass spectrometer during the void time of the gas chromatograph and to calculate the mass calibration or the lock mass correction to be used by the sample analysis logic.
A method of performing mass spectrometry includes obtaining, based on a series of mass spectra of detected ions derived from components eluting from a chromatography column, a plurality of extracted ion chromatograms (XICs), each XIC comprising a plurality of detection points representing detected intensity for a distinct selected m/z as a function of time; detecting, based on the series of mass spectra, precursor ions of each distinct selected m/z of the plurality of XICs; and determining, for each XIC based on a set of detection points of the XIC, a predicted next detection point to be obtained based on a next mass spectrum to be acquired.
A mass spectrometer system includes a vacuum manifold; an ion source positioned within the vacuum manifold for ionizing a sample; a mass analyzer for analyzing sample ions; a high vacuum pump connected to the vacuum manifold operable to maintain the pressure within the vacuum manifold at an operating pressure; and a controller configured to raise the pressure in the ion source to a sputtering pressure by supplying a flow of a sputtering gas and either reducing a speed of a high vacuum pump or isolating the ion source from the high vacuum pump; cause a conducting material to be sputtered on a surface of the ion source; and reduce the pressure in the ion source to an operating pressure by reducing the flow of the sputtering gas and either increasing the speed of the high vacuum pump or restoring connectivity between the ion source and the high vacuum pump.
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
C23C 14/06 - Revêtement par évaporation sous vide, pulvérisation cathodique ou implantation d'ions du matériau composant le revêtement caractérisé par le matériau de revêtement
C23C 14/14 - Matériau métallique, bore ou silicium
A system for performing dynamic DIA directs a mass spectrometer to acquire, based on an acquisition schedule that schedules a plurality of acquisition cycles, MS2 spectra of product ions derived from analytes included in a sample as the analytes elute from a separation system. The acquisition schedule specifies, for each acquisition cycle included in the plurality of acquisition cycles, a dynamic precursor m/z range based on an expected elution time of the analytes. The product ions are produced from precursor ions isolated using an isolation window successively positioned throughout the dynamic precursor m/z range during each acquisition cycle. The system detects an elution time shift in the elution time of the analytes as the analytes elute and adjusts the acquisition schedule based on the detected elution time shift.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus, includes timing logic to determine a maximum time without wetting for a syringe; and rinsing logic to control an autosampler to rinse the syringe when the maximum time without wetting is reached.
Methods are described for the automatic determination and correction of retention time shift of a MS data set relative to a control data set, to correct for retention time drifts endemic to targeted LCMS analyses. In an embodiment, a 2D grid of periodic MS spectra versus time is collected for a control experiment, and RT windows are determined with an additional set of unscheduled mass spectral analyses. During successive experiments, spectra from periodic MS scans are used to determine the correspondence between the current time and the time in the control experiment. The active set of MSn scans to be acquired by the instrument is then determined as the scans with adjusted retention time windows that bracket the corrected retention time.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a method is provided that includes receiving a first plurality of experiment parameters for a first experiment to be performed by a scientific instrument on a first sample, the first plurality of experiment parameters including a list, and storing the list. The method further includes receiving a second plurality of experiment parameters for a second experiment to be performed by the scientific instrument, the second plurality of experiment parameters including a selection, within a graphical user interface, of the list from the first experiment to reuse for the second experiment. The method further includes receiving experiment data relating to the second experiment and analyzing the experiment data based on the list from the first experiment to determine a result of the second experiment.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a method is provided that includes receiving a first plurality of experiment parameters for a first experiment to be performed by a scientific instrument on a first sample, the first plurality of experiment parameters including a list, and storing the list. The method further includes receiving a second plurality of experiment parameters for a second experiment to be performed by the scientific instrument, the second plurality of experiment parameters including a selection, within a graphical user interface, of the list from the first experiment to reuse for the second experiment. The method further includes receiving experiment data relating to the second experiment and analyzing the experiment data based on the list from the first experiment to determine a result of the second experiment.
G06F 3/0484 - Techniques d’interaction fondées sur les interfaces utilisateur graphiques [GUI] pour la commande de fonctions ou d’opérations spécifiques, p. ex. sélection ou transformation d’un objet, d’une image ou d’un élément de texte affiché, détermination d’une valeur de paramètre ou sélection d’une plage de valeurs
G06F 3/0482 - Interaction avec des listes d’éléments sélectionnables, p. ex. des menus
80.
ION-TO-ELECTRON CONVERSION DYNODE FOR ION IMAGING APPLICATIONS
A metal-channel conversion dynode comprises: a wafer comprising a first face and a second face parallel to the first face and having a thickness less than 1000 μm; and a plurality of channels passing through the wafer from the first face to the second face at an angle to a plane of the first face and a plane of the second face. In some embodiments, each inter-channel distance may be substantially the same as the wafer thickness. In some embodiments, the wafer is fabricated from tungsten. In some other embodiments, the wafer comprises a non-electrically conductive material that is fabricated by three-dimensional (3D) printing or other means and that is coated, on its faces and within its channels, with a metal or suitably conductive coating that produces secondary electrons upon impact by either positive or negative ions.
An embodiment of the present invention provides an RF ion guide having four elongated electrodes arranged in parallel around the axial centerline. Each electrode is generally L-shaped in cross section, having first and second inner surfaces directed toward the interior of the ion guide. The first and second surfaces extend along axis that are transverse and preferably approximately perpendicular to one another. RF voltages of equal amplitude but opposite phases are applied to opposed pairs of electrodes, in the manner known in the art, to generate an RF field to radially confine ions and focus them to the centerline. Because the resultant RF field more closely approximates a quadrupolar field, relative to the field generated within a flatapole, better performance may be achieved in terms of improved transmission efficiencies and/or less mass discrimination.
This system and method disclosed herein are configured to improve high mass range ion trap performance by use of a multi-directional segmented scan approach. In some embodiments of the system and method disclosed herein, the mass range of conventional ion trap technology may be extended/increased without changing the hardware or compromising lower range mass/charge efficiency. Specifically, the system and methods disclosed herein use a segmented, bi-directional scan that increases the mass range of an ion trap mass spectrometer and circumvents the problem of mass discrimination during mass analysis in the high Thompson value range.
Disclosed herein are charge state deconvolution systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a charge state deconvolution apparatus includes first logic to identify peaks in a mass spectrum; second logic to deconvolve the masses of the identified peaks and identify clusters of deconvolved mases that have contiguous charge states; and third logic to calculate a Bayesian fitness measure and perform an iterative decremental procedure to perform charge state deconvolution.
G06F 18/2415 - Techniques de classification relatives au modèle de classification, p. ex. approches paramétriques ou non paramétriques basées sur des modèles paramétriques ou probabilistes, p. ex. basées sur un rapport de vraisemblance ou un taux de faux positifs par rapport à un taux de faux négatifs
85.
Ion guide electrode configurations for polarity-independent ion containment
An ion guide includes a first arrangement of electrodes on a first surface, a second arrangement of electrodes on a second surface, and an ion containment space in a gap therebetween. The first arrangement includes first electrodes and second electrodes. Each first electrode includes a first main portion and a first edge portion. The first edge portion is wider than the first main portion. The second arrangement includes third electrodes and fourth electrodes. Each fourth electrode includes a fourth main portion and a fourth edge portion. The fourth edge portion is wider than the fourth main portion. The first edge portions are positioned opposite the fourth edge portions. The first electrodes and the third electrodes are configured to receive first RF voltages and the second electrodes and the fourth electrodes are configured to receive second RF voltages that are phase-shifted with respect to the first RF voltages.
H01J 3/40 - Pièges pour supprimer ou détourner des particules indésirables, p. ex. des ions négatifs, des électrons en margeDispositifs sélecteurs de vitesse ou de masse
H01J 49/06 - Dispositifs électronoptiques ou ionoptiques
H01J 49/42 - Spectromètres à stabilité de trajectoire, p. ex. monopôles, quadripôles, multipôles, farvitrons
86.
Removable Ion Source Capable Of Axial Or Cross Beam Ionization
An ion source including an ionization assembly, first and second electron sources, and a magnet assembly. The ionization assembly includes an ionization chamber and at least one ion lens. The ionization assembly has a primary axis defined by the direction of an ion beam exiting the ionization assembly and the ionization chamber and the at least one ion lens are arranged along the primary axis. The first electron source is aligned along the primary axis of the ionization assembly and is configured to provide an electron beam parallel to the primary axis. The second electron source is adjacent to the ionization assembly and is configured to provide an electron beam orthogonal to the primary axis. The magnet assembly includes a magnet. The magnet assembly is movable between a first position in which the magnet is aligned with the first electron source and a second position in which the magnet is aligned with the second electron source.
A system including a gas source coupled to a mass spectrometer with a supply line to provide reagent gas for chemical ionization; a bypass line connecting the supply line to a foreline of a vacuum pump, the bypass line including a valve and a bypass restrictor; and a cycle timer operable to open the valve for a first period of time and close the valve for a second period of time.
A Liquid Chromatography Mass Spectrometry system comprises: a chromatograph; a mass spectrometer configured to ionize separated fractions of a sample received from the chromatograph; and a programmable processor operable to repeatedly execute the steps of: (i) causing the mass spectrometer to perform a data-independent analysis of the precursor ion species using a mass analyzer of the mass spectrometer; (ii) calculating one or more degree-of-matching scores that relate to detection of an internal standard; and (iii) if each of the degree-of-matching scores meets a respective degree-of-matching condition, performing a quantitative tandem mass spectrometric analyses of both the internal standard and the analyte; the programmable processor further operable to calculate a quantity of the analyte in the sample by comparison between intensities of one or more mass spectral signals generated by the quantitative tandem mass spectrometric analyses of the analyte and the internal standard.
G01N 30/00 - Recherche ou analyse de matériaux par séparation en constituants utilisant l'adsorption, l'absorption ou des phénomènes similaires ou utilisant l'échange d'ions, p. ex. la chromatographie
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/42 - Spectromètres à stabilité de trajectoire, p. ex. monopôles, quadripôles, multipôles, farvitrons
A mass spectrometer includes an ionization assembly including an ionization chamber and at least one ion lens. The removable ionization assembly has a primary axis defined by the direction of an ion beam exiting the ionization assembly, and the ionization chamber and the at least one ion lens arranged along the primary axis. The mass spectrometer further includes an electron source aligned along the primary axis of the ionization assembly and a magnet assembly including a magnet. The electron source configured to provide an electron beam parallel to the primary axis. The magnet assembly movable between a first position in which the magnet is positioned to allow removal of the ion source and a second position in which the magnet is aligned with the electron source.
A mass spectrometry method comprises: choosing an RF drive frequency that best optimizes isolation of a precursor ion species of interest by a quadrupole mass filter (QMF); isolating the precursor ion species by passing ions through the QMF while the chosen RF drive frequency is applied thereto; fragmenting the precursor ion species, thereby generating a plurality of first-generation fragment ion species; returning the plurality of first-generation fragment ion species to an inlet end of the QMF; choosing a second quadrupole RF drive frequency that best optimizes isolation of a first-generation fragment ion species of interest by the QMF; isolating the first-generation fragment ion species of interest by passing the fragment ions through the QMF while the second chosen RF drive frequency is applied thereto; fragmenting the chosen first-generation fragment ion species of interest, thereby generating a plurality of second-generation fragment ion species; and mass analyzing the second-generation fragment ion species.
A method of performing mass spectrometry includes accessing a series of mass spectra of detected ions derived from components eluting from a chromatography column; obtaining, based on the series of mass spectra, an elution profile including a plurality of detection points representing intensity of at least a set of the detected ions as a function of time; and determining, based on a set of detection points included in the plurality of detection points, a predicted next detection point of the elution profile to be obtained based on a next mass spectrum to be acquired subsequent to acquisition of the series of mass spectra.
A multipole ion guide includes a plurality of electrodes disposed about a longitudinal axis of the device so as to define an ion transmission volume for transmitting ions along a length of the device between opposite inlet and outlet ends. An electronic controller is operably connected to an RF power source and to at least some of the electrodes and is configured to apply at least an RF potential to the electrodes. During use the electrodes generate an RF-only field along a first portion of the device and an axial DC field along a second portion of the device. Ions are focused radially inward toward the longitudinal axis of the device by the RF-only field within the first portion of the device prior to and/or subsequent to experiencing the axial DC field within the second portion of the device.
Disclosed herein are systems and methods for a mass spectrometer having a multipole configured to pass an ion stream, and a detector configured to detect the properties of the abundance of ions represented by data points. The mass spectrometer also includes a processing system that is configured to obtain a plurality of paired data points (e.g., detector data points and RF amplitude data points), and identify, based on centroiding a portion of the plurality of paired data points, at least one characteristic of a peak and determine, based on the at least one characteristic of the peak, a preferred peak shape.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a scientific instrument support apparatus includes evaluation logic to identify a first syringe has a bent needle, a bent plunger, a blocked needle, or a stuck plunger; syringe logic to switch from the first syringe to a second syringe; and reporting logic to notify a user of a syringe problem.
A61M 5/168 - Moyens pour commander l'écoulement des agents vers le corps ou pour doser les agents à introduire dans le corps, p. ex. compteurs de goutte-à-goutte
G16H 20/17 - TIC spécialement adaptées aux thérapies ou aux plans d’amélioration de la santé, p. ex. pour manier les prescriptions, orienter la thérapie ou surveiller l’observance par les patients concernant des médicaments ou des médications, p. ex. pour s’assurer de l’administration correcte aux patients administrés par perfusion ou injection
A61M 5/20 - Seringues automatiques, p. ex. avec tige de piston actionnée automatiquement, avec injection automatique de l'aiguille, à remplissage automatique
95.
METHOD FOR QUANTITATIVE MONITORING OF MRNA CAPPING EFFICIENCY
A method for quantifying mRNA capping efficiency includes combining a sample, an enzyme mixture, and an isotopic standard solution in a buffer solution to create an incubation mixture, the enzyme mixture including a non-specific, single-stranded nuclease and an acid phosphatase and the isotopic standard including isotopically labeled m7G and isotopically labeled 2'-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of a capping efficiency and a 2-O-methyltransferase efficiency.
A system for analyzing a sample includes a source configured to generate ions from constituent components of the sample; a mobility separator configured to separate ions received from the source based on the mobility in a gas; a ion storage array configured to store ions from the mobility separator as a plurality of mobility fractions; a mass filter configured to select ions within a mass-to-charge window; a mass analyzer configured to determine the mass-to-charge ratio of the ions; and a controller. The controller is configured to identify an ion mobility fraction and a mass-to-charge window to select for a charge state or ion class; utilize the mass filter to select ions from the ion storage array within the mass-to-charge window corresponding to a charge state or ion class; and analyze the selected ions with the mass analyzer.
It is proposed to improve transmission of ions along the curved path of an ion guide by changing the separation between adjacent multipole rods in the plane of curvature. As the separation increases along the length of the device, the ion confinement in the plane of curvature decreases. At the same time, the external field penetration from the electrodes outside of the main ion guide increases which can be used to create additional DC field gradients to facilitate ion confinement and motion through the ion guide.
It is proposed to improve transmission of ions along the curved path of an ion guide by changing the separation between adjacent multipole rods in the plane of curvature. As the separation increases along the length of the device, the ion confinement in the plane of curvature decreases. At the same time, the external field penetration from the electrodes outside of the main ion guide increases which can be used to create additional DC field gradients to facilitate ion confinement and motion through the ion guide.
A ferrule includes a deformable portion and a rigid sleeve surrounding at least a portion of the deformable portion. The deformable portion includes between about 5% and about 50% graphite. The deformable portion further includes between about 50% and about 95% polyimide. The rigid sleeve can include a metal, such as stainless steel.
A mass selective ion trapping device includes a linear ion trap and a RF control circuitry. The ion trap includes a plurality of trap electrodes configured for generating a quadrupolar trapping field in a trap interior and for mass selective ejection of ions from the trap interior. The RF control circuitry is configured to apply a balanced AC voltage to the trap electrodes during a first period of time such that an AC voltage applied to a first pair of trap electrodes is of the same magnitude and of opposite sign to an AC voltage applied to a second pair of trap electrodes; apply unbalanced RF voltage to the second pair of trap electrodes during a second period of time; ramp the balanced AC voltage down and the unbalanced RF voltage up during a transition period; and eject ions from the linear ion trap after the second period of time.
