Abrégé

This non-specific reaction inhibitor used for biochemical measurement comprises as active ingredients: (a) an antibody or a fragment thereof; and (b) a copolymer containing (1) a first repeating unit having a linear or branched alkyl group having 12-40 carbon atoms and (2) a second repeating unit having a cationic moiety and an anionic moiety.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/531 - Production de matériaux de tests immunochimiques

Abrégé

Provided is an ultrasensitive method for measuring an anti-drug antibody that is simpler and less expensive than conventional methods. Provided is an ultrasensitive method for measuring an analyte using a capture probe and an assist probe and adopting an improved PALSAR method. By using the capture probe and the assist probe and adopting the improved PALSAR method in a double antigen bridging immunoassay, ultrasensitive measurement of an anti-drug antibody can be performed simply and inexpensively.

Classes IPC  ?

• C12Q 1/682 - Amplification du signal
• C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes

Abrégé

An information presentation device comprises: one or more imaging units that are provided to image a container disposed at a predetermined collection position; an image acquisition unit that acquires captured images that obtained by the imaging unit performing imaging at one or more predetermined timings in a unit collection period from when the container containing the sample is disposed at the collection position to when a sample probe that has collected the sample is positioned outside the container; a determination unit that makes a determination regarding at least one of an abnormality that occurs in response to the collection of the sample in the unit collection period or an abnormality in a measurement result for the collected sample; and an information presentation unit that, when presenting abnormality-related information related to the abnormality determined by the determination unit, includes the captured images, acquired by the image acquisition unit, in the abnormality-related information in accordance with the unit collection period in which abnormalities of an object occurred.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

This immunoassay method comprises: a step for detecting a signal corresponding to the concentration or titer of an antigen contained in a biological sample; and a step for calculating the concentration or titer of the antigen in the biological sample from the signal by using a calibration curve indicating the correlation between the signal and the concentration or titer of the antigen. The calibration curve is created using a standard sample containing a fusion protein. The fusion protein has an amino acid sequence of at least a portion of the antigen and an amino acid sequence of at least a portion of the constant domain of immunoglobulin.

Classes IPC  ?

• G01N 33/532 - Production de composés immunochimiques marqués
• C07K 14/78 - Peptides du tissu connectif, p. ex. collagène, élastine, laminine, fibronectine, vitronectine ou globuline insoluble à froid [CIG]
• C07K 16/00 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux
• C07K 19/00 - Peptides hybrides
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

This immunoassay reagent includes a labeled antibody, an insoluble carrier, and a non-specific adsorption inhibitor. The labeled antibody includes a first antibody, a hydrophobic first linker, and a labeling substance. The labeling substance is bound to the first antibody via the first linker. The non-specific adsorption inhibitor includes a hydrophobic second linker and a hydrophilic part bonded to the second linker.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

This nonspecific reaction inhibitor is a copolymer containing a first repeating unit represented by formula (1) and a second repeating unit represented by formula (2). In formula (1), R1, R2, and R333 -or COO-, and Yn and m are each independently an integer from 1 to 5. In formula (2), R4 is a hydrogen atom or a methyl group, Z is NH or an oxygen atom, and A is a linear or branched alkyl group with 12 to 40 carbon atoms.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• C08F 220/10 - Esters
• C08F 220/60 - Amides contenant de l'azote en plus de l'azote de la fonction carbonamide
• G01N 33/531 - Production de matériaux de tests immunochimiques

Abrégé

Provided are: a reagent composition for measuring activated partial thromboplastin time, the reagent composition containing an antibody that binds to a blood coagulation factor as an antigen or an antigen-binding fragment thereof; and an inspection method including a step for mixing the reagent composition and a plasma specimen to be inspected at a prescribed ratio and measuring if the blood coagulation time thereof is T, and a step for determining that the plasma specimen is abnormal when the blood coagulation time T is longer than a designated reference.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

This determination device comprises: an information acquisition unit that acquires information used for making a determination indicating a prescribed condition of a target piercer on the basis of a captured image obtained as a result of imaging by an imaging unit provided so as to image the target piercer that is provided to a measurement unit and is in a prescribed position condition; and a determination unit that makes a determination pertaining to wear of the target piercer on the basis of the information used for making a determination acquired by the information acquisition unit.

Classes IPC  ?

• G01N 21/84 - Systèmes spécialement adaptés à des applications particulières
• G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection

Abrégé

Provided is a blood collection container which can suppress the release of exosomes from platelets, and therefore, can suppress the contamination of plasma by exosomes derived from platelets.　A blood collection container according to the present invention is configured so as to collect a predetermined amount of blood, and comprises: a blood collection container body; a plasma separation material stored in the blood collection container body; and an aqueous solution stored in the blood collection container body. The aqueous solution contains an anticoagulant. When a specific pH is measured, the pH of a specific mixed liquid (X) is 3.5-5.5.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

Provided is a blood collection container capable of reducing the amount of white blood cells mixed into plasma even when blood is centrifuged several days after collection of the blood.　The blood collection container according to the present invention comprises: a blood collection container body; a plasma separation material stored in the blood collection container body; and an aqueous solution stored in the blood collection container body, wherein the aqueous solution contains an anticoagulant and contains a lithium salt different from the anticoagulant.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• G01N 1/10 - Dispositifs pour prélever des échantillons à l'état liquide ou fluide

Abrégé

This information presentation device is constituted by including a display control unit that, on the basis of measurement results obtained by measuring a plurality of measurement items for each of one or more specimens, displays one or more representational bodies in association with each specimen that represent information based on the measurement results, together with a measurement value indicating a measurement result for each of the plurality of measurement items.

Classes IPC  ?

• G06F 3/0482 - Interaction avec des listes d’éléments sélectionnables, p. ex. des menus
• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang
• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

A qualitative or quantitative test method to detect a substance to be detected contained in a specimen, the test method including a preparation step of preparing a test sample from the specimen, and a test step of testing at least part of the test sample with a reagent to detect each of a first substance to be detected and a second substance to be detected, in which the first substance to be detected has an isoelectric point of 10.0 or more, and in any one or both of the preparation step and the test step, the first substance to be detected is brought into contact with any one or both of a glass-made member and a nitrocellulose-made member in the presence of a cationic substance. In this test method, two or more substances to be detected including a substance having an isoelectric point of 10.0 or more can be detected with high sensitivity using the same specimen while suppressing a decrease in measurement sensitivity.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is an immunological measurement method for measuring a target substance in a sample, wherein an antigen-antibody reaction is performed at least once in the presence of an enzyme that specifically decomposes immunoglobulin A, abbreviated as IgA.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

A method for measuring an oligonucleotide which is simpler and more sensitive and has excellent specificity and quantitative capability compared to the conventional measurement method is provided. Moreover, a method for measuring an oligonucleotide having excellent specificity which can distinguish the intact target oligonucleotide (unchanged form) and a metabolite thereof and detect the unchanged form only is provided. In a hybridization method using a capture probe and an assist probe, by using a capture probe having a short nucleotide length in a certain range and the assist probe and causing hybridization under a specific positional relationship between the nucleotide-lacking-site in a metabolite of a nucleic acid drug and the capture probe, it becomes possible not only to detect the target oligonucleotide in a sample but also to distinguish from a metabolite of the nucleic acid drug.

Classes IPC  ?

• C12Q 1/682 - Amplification du signal
• C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes

Abrégé

An immunological analysis method, including a process of binding an antibody to an antigen, the antibody being included in a complex, and the complex including a labeling substance and having a configuration in which plural antibodies are bound to each other via a scaffold compound.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/532 - Production de composés immunochimiques marqués

Abrégé

Provided is a coagulation time extension factor estimation method, comprising: 1) obtaining Tm(X) and Tn(X); and 2) estimating a coagulation time extension factor for a test blood sample on the basis of Tm(X), wherein Tm(X) represents a measurement point or a time at which a coagulation reaction curve of sample M reaches X % of coagulation reaction end point Em, Tn(X) represents a measurement point or a time at which a coagulation reaction curve of sample N reaches X % of coagulation reaction end point En, Em is a coagulation reaction end point in the coagulation reaction curve of sample M, En is a coagulation reaction end point in the coagulation reaction curve of sample N, the sample S is a test blood sample having an extended coagulation time, the sample N is a normal blood sample, the sample M is a sample mixture of the sample S and the sample N, and X is larger than 0 and 100 or smaller.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

An objective of the present invention is to provide a technique for increasing sensitivity of latex immunoagglutination reagents while inhibiting sedimentation of particles in a latex particle dispersion liquid. In particular, it is to provide the latex particle dispersion liquid which can be applied to the latex immunoagglutination reagents with adequate sensitivity while inhibiting occurrence of agglutination over time in the latex particle dispersion liquid having a large particle diameter. That is, it is to provide the latex particle dispersion liquid using an aqueous medium as a dispersion medium, and including one or more compounds selected from the group consisting of polyacrylic acid, sodium polyacrylate, polyethylene oxide, and sodium poly-γ-glutamate. It is also to provide immunoagglutination reagents to which the latex particle dispersion liquid is applied.

Classes IPC  ?

• G01N 33/547 - Résine synthétique avec un antigène ou un anticorps liés au support par l'intermédiaire d'un agent de pontage
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

In a specimen processing system, plural pieces of accuracy control data that is time series data of an accuracy control value obtained by analyzing an accuracy control sample by an analysis unit is stored in an accuracy control measurement information database. Then, the display control unit simultaneously displays the accuracy control data of plural accuracy control samples of the same type and different production lot numbers on a touch display based on the accuracy control data stored in the accuracy control measurement information database.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

Provided are: a recording unit that records one or more measurement items performed on a specimen put in at least one specimen container and a measurement order of each of the measurement items; a specimen dispensing mechanism that dispenses the specimen selected as a measurement target based on the measurement order into at least one of a first reaction container or a second reaction container; a first measurement unit that performs coagulation measurement, which is measurement of a coagulation item, on the specimen dispensed into the first reaction container after completion of a first preparation process determined in advance; and a second measurement unit that performs specific measurement, which is the measurement item different from the measurement of the coagulation item, on the specimen dispensed into the second reaction container after completion of a second preparation process determined in advance.

Classes IPC  ?

• G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang
• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
• G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
• G01N 35/04 - Détails du transporteur

Abrégé

An immunoassay reagent, including: a substance that exhibits an immune reaction; and an insoluble carrier having a function to bind to the substance, the insoluble carrier including an insoluble carrier that is bound to the substance and an insoluble carrier that is not bound to the substance.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

In the specimen processing system, reagent information regarding a reagent including position information of the reagent is stored for each of the reagents in the reagent information database. Then, based on the position information of the reagent included in the reagent information, the display control unit causes a reagent information display screen to be displayed on a touch display of a terminal, the reagent information display screen including a reagent recognition mark display region that displays a reagent recognition mark corresponding to the reagent arranged in the reagent cooling storage so as to correspond to an arrangement position of the reagent.

Classes IPC  ?

• G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

Provided is a circulating tumor cell isolation kit capable of increasing a recovery rate of circulating tumor cells not only when a specimen for which a long time has not elapsed since blood collection is used but also when a specimen stored for several days after blood collection is used. The circulating tumor cell isolation kit according to the present invention is a kit used for isolation of circulating tumor cells in blood, the kit including: a blood collection container accommodating an aqueous solution and collecting a predetermined amount of blood; and a cell isolation container accommodating a cell isolation material having a specific gravity at 25° C. of 1.065 to 1.080, the aqueous solution containing an anticoagulant and containing a low molecular weight compound having a molecular weight of 75 to 500 or a high molecular weight compound having a number average molecular weight of 2,000 or more and less than 200,000, and when physiological saline in an amount equivalent to the predetermined amount of blood collected in the blood collection container is collected in the blood collection container to obtain a mixed liquid in which the physiological saline and the aqueous solution are mixed, an osmotic pressure of the mixed liquid being 270 to 350 mOsm/L.

Classes IPC  ?

• C12N 5/09 - Cellules tumorales
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet

Abrégé

A method for measuring an oligonucleotide which is simpler and more sensitive and has excellent specificity and quantitative capability compared to the conventional measurement method is provided. Moreover, a method for measuring an oligonucleotide having excellent specificity which can distinguish the intact target oligonucleotide (unchanged form) and a metabolite thereof and detect the unchanged form only is provided. In a hybridization method using a capture probe and an assist probe, by inserting a spacer between a solid phase and a nucleic acid probe contained in the capture probe, it becomes possible not only to detect the target oligonucleotide in a sample but also to distinguish from a metabolite of a nucleic acid drug.

Classes IPC  ?

• C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes

Abrégé

An immunoassay method for cross-linked N-telopeptide of type I collagen, comprising a step of contacting a biological sample with an antibody or antibody fragment thereof that binds to a peptide fragment having an amino acid sequence represented by JYDGKGVG (SEQ ID NO: 1). This method is easy in operation and enable NTx measurement with higher accuracy.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

The present invention provides an immunological detection method for trimeric type I collagen N-terminal propeptide in a biological sample, which uses a first antibody that binds to a first specific portion of a pro-al chain in the trimeric type I collagen N-terminal propeptide and a second antibody that binds to a second specific portion of a pro-al chain in the trimeric type I collagen N-terminal propeptide. The immunological detection method is easy to handle and can specifically (selectively) measure the trimer in biological samples containing the trimer and monomer of the α-chain of PINP.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées

Abrégé

The present invention provides an immunological detection method for trimeric type I collagen N-terminal propeptide in a biological sample, which uses a first antibody that binds to a specific portion of a pro-α1 chain in the trimeric type I collagen N-terminal propeptide and a second antibody that binds to a specific portion of a pro-α2 chain in the trimeric type I collagen N-terminal propeptide. The immunological detection method is easy to handle and can specifically (selectively) measure the trimer in biological samples containing the trimer and monomer of the α-chain of PINP.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées

Abrégé

Provided is a method for estimating a factor causing an abnormality in coagulation of a blood sample. In the method, 1) a parameter Ps relating to a coagulation reaction in a sample S and a parameter Pm relating to a coagulation reaction in a sample M are calculated, 2) an estimation as to whether the sample S is coagulation-factor-inhibitor-positive is carried out on the basis of Ps, and 3) a sample S that is not estimated to be coagulation-factor-inhibitor-positive in 2) is estimated to be lupus-anticoagulant-positive or coagulation-factor-deficient on the basis of Pm or on the basis of Ps and Pm. The sample S is a test blood sample having an extended coagulation time, the sample M is a mixed sample of the sample S and a sample N, and the sample N is a normal blood sample. Ps is calculated on the basis of a measurement point or time at which a coagulation reaction curve or a coagulation speed curve of the sample S reaches a prescribed value, and Pm is calculated on the basis of a measurement point or time at which a coagulation reaction curve or a coagulation speed curve of the sample M reaches a prescribed value.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

The present invention relates to a latex particle dispersion containing latex particles and an aqueous medium, the latex particle dispersion being characterized by further containing at least one type of compound selected from the group consisting of hexametaphosphoric acid and salts thereof.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/531 - Production de matériaux de tests immunochimiques

Abrégé

Provided is a blood collection container capable of suppressing contamination of plasma with leukocyte-derived DNA during specimen storage. A blood collection container according to the present invention includes a blood collection container main body, a plasma separation material contained in the blood collection container main body, and an aqueous solution contained in the blood collection container main body, wherein the plasma separation material has a specific gravity at 25° C. of 1.027 or more and 1.060 or less, and the aqueous solution contains an anticoagulant, as well as contains a water-soluble polymer compound having a number average molecular weight of 500 or more and 180,000 or less or ammonium sulfate.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• G01N 1/10 - Dispositifs pour prélever des échantillons à l'état liquide ou fluide
• G01N 1/40 - Concentration des échantillons
• G01N 33/49 - Analyse physique de matériau biologique de matériau biologique liquide de sang

Abrégé

Provided is an immunological measurement method for measuring a substance to be measured in a sample, wherein an antigen-antibody reaction is performed at least once in the presence of an enzyme specifically decomposing immunoglobulin M, abbreviated as IgM.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

It is an object of the present invention to provide a method for suppressing non-specific reactions using a highly versatile method without affecting specific reactions. The present invention provides a detection method for detecting a target antigen in a sample using a specific antibody that specifically binds to the antigen, the method comprising: a) a step of bringing the sample into contact with a modified antibody; and b) a step of bringing the sample into contact with the specific antibody simultaneously with or after the step a), which is a highly versatile method that can suppress non-specific reactions.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/536 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide

Abrégé

The present invention provides an immunochromatography test strip for detecting a detection target substance, comprising: a sample supply section to which a sample potentially containing the detection target substance is supplied; a conjugate section containing a conjugate in which a label is bound to an antibody or antigen that immunologically reacts with the detection target substance; and a detector section that captures a complex containing the detection target substance and the conjugate, wherein the detection target substance is a protein with an isoelectric point of 9.5 or higher, and either or both of the sample supply section and the conjugate section are formed on a polyester fiber pad or a polyolefin fiber pad.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 1/40 - Concentration des échantillons
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

A method for analyzing a blood coagulation reaction comprises, by a blood coagulation analysis device, measuring a coagulation reaction of a sample solution including a subject blood specimen, determining whether or not the coagulation reaction of the sample solution is completed, and when it is determined that the coagulation reaction is completed, terminating measurement of the coagulation reaction of the sample solution. The blood coagulation analysis device includes a reaction table which is rotated in one direction, and the reaction table includes a measurement port to which a cell can be attached. The coagulation reaction of the sample solution in the cell supplied to the measurement port is measured, and time series data of the measured coagulation reaction is accumulated. Whether or not the coagulation reaction is completed is determined from the accumulated time series data of the coagulation reaction, and when it is determined that the coagulation reaction is completed, measurement of the coagulation reaction of the sample solution is terminated, and the cell containing the sample solution is removed from the measurement port.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

Provided is a method for estimating a cause of a coagulation anomaly in a blood specimen, said method comprising calculating a deviation curve D(i) of a subject blood specimen, calculating a parameter which reflects the shape of said D(i), and estimating a cause of a coagulation anomaly in said subject blood specimen on the basis of said parameter, wherein D(i)=P(i)-Pn(i) where: i represents the number of measured points or time; P(i) is a curve obtained by converting measurement data of a coagulation reaction of said subject blood specimen into a relative value so that the minimum value is 0, and the maximum value is A; Pn(i) is a curve obtained by shifting P'(i) so that a rise point thereof is aligned with a prescribed point X, where said P'(i) is a curve obtained by converting measurement data of a coagulation reaction of a reference specimen into a relative value so that the minimum value is 0, and the maximum value is A; A>0; and X is 5-15 seconds or the number of measured points corresponding thereto.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

A blood coagulation test reagent containing a recombinant tissue factor produced using Gram-positive bacteria.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

The present invention provides an immunoassay method for detecting a protein with an isoelectric point of 9.5 or higher, comprising a step of bringing a sample containing the protein with an isoelectric point of 9.5 or higher into contact with a glass material in the presence of a cationic substance, and an immunochromatography kit for detecting a protein with an isoelectric point of 9.5 or higher, comprising a specimen diluent and an immunochromatography test strip.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées

Abrégé

A measurement reagent for a cross-linked N-telopeptide of type I collagen containing uric acid or its salt. This reagent is easier to handle and enables NTx measurement with higher accuracy.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

A method for detecting an anomaly in a blood coagulation reaction, including: 1) detecting a coagulation reaction end point Pe in a coagulation reaction curve of a subject blood specimen; 2) calculating T(X), where T(X) represents a measurement point or time at which the coagulation reaction curve reaches X % of Pe and X denotes a variable larger than 0 and equal to or smaller than 100; and 3) detecting the anomaly in the blood coagulation reaction of the subject blood specimen based on T(X).

Classes IPC  ?

• G01N 33/49 - Analyse physique de matériau biologique de matériau biologique liquide de sang

Abrégé

Provided is a storage solution for a cell-containing solution capable of suppressing leakage of genomic DNA to the outside of cells when a mixed solution of a cell-containing solution and a storage solution is stored under refrigeration. A storage solution for a cell-containing solution according to the present invention contains a formaldehyde donor compound (A) and a compound (B) that is an inorganic salt, an organic acid, or an organic salt, an electrical conductivity of the storage solution being 3 ms/cm or more and 20 ms/cm or less.

Classes IPC  ?

• A01N 1/02 - Conservation de parties vivantes
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]

Abrégé

A microchannel device has a first channel system including a liquid holder and a second channel system including a dry reagent holder, with the first channel system and the second channel system being shut off from each other in a chip body. The lid member has a first groove in a contact surface to face the chip body. The lid member, when attached to the chip body, allows the first channel system and the second channel system to communicate with each other via the first groove.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

Provided is a blood collection container with which the collection amount of mononuclear cells can be increased.　The blood collection container according to the present invention comprises a blood collection container body, a baric solution accommodated in the blood collection container body, and a blood separation material accommodated in the blood collection container body, wherein the baric solution contains an amino acid.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

Provided are an automatic analysis method and automatic analysis device that improve the accuracy of measurement data by correcting various measurement errors including errors caused by individual differences among automatic analysis devices.　The present invention improves measurement accuracy by means of an automatic analysis method in which an examination target and a reagent containing a labeling substance are received, the amount of light obtained from the labeling substance is measured, and the examination target is qualitatively or quantitatively measured, wherein: a light calibration curve is created for each of a plurality of automatic analysis devices on the basis of data obtained by digitally measuring a plurality of samples using each of the automatic analysis devices and data obtained by separate analog measurement of said samples; the relevant light calibration curve is used to convert a digital measurement value obtained by measuring the examination target serving as the subject of analysis into a digital estimation value; and a measurement target in the examination target is measured on the basis of the digital estimation value.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
• G01N 21/76 - ChimioluminescenceBioluminescence

Abrégé

Provided are an analysis method for an automated analysis device, and the automated analysis device, with which it is possible to construct a highly accurate calibration curve that can be used over a wide range up to a high concentration region, using a small number of calibration points.　An analysis method for an automated analysis device according to the present invention uses a calibration curve created by calibration performed using a calibrator adjusted in advance to a known concentration, to convert a measurement result, obtained from the analysis device by reacting a reagent corresponding to an analysis item with a specimen, into a concentration value, in order to analyze a component to be measured contained in the specimen. The calibration curve is created using two or more calibrator measurement values C0, C1 located in a linear region S and one or more unique calibrator measurement values CP located in a convergence region P, and a concentration value. In this case, the unique calibrator measurement value Cp located in the convergence region P is one that is held in advance by the analysis device.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
• G01N 27/416 - Systèmes

Abrégé

The present disclosure provides: a filler for ion exchange chromatography, the filler having an extremely high level of separation performance even when the column pressure is reduced, and being useful particularly for the measurement of a hemoglobin component such as hemoglobin A1c; and a production method which makes it possible to produce the filler with high efficiency and in a simple manner. The present disclosure also provides a method for measuring a hemoglobin component, the method making it possible to measure a hemoglobin component with extremely high accuracy. The filler according to the present disclosure is intended to be used in ion exchange chromatography. The filler comprises core-shell-type particles each having a core part and a shell part that covers at least a portion of the core part. Each of the core-shell-type particles has a cation exchange group on the surface of the shell part. With respect to the core-shell-type particles, a CV value expressed by formula (1): a CV value = ((standard deviation of particle diameter)/(average particle diameter))×100 is 18% or less.

Classes IPC  ?

• B01J 20/281 - Absorbants ou adsorbants spécialement adaptés pour la chromatographie préparative, analytique ou de recherche
• G01N 30/02 - Chromatographie sur colonne
• G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes

Abrégé

The automatic analysis device of the invention includes a history information storage unit configured to store history information related to a previously conducted analysis, a number-of-analyses calculation unit configured to calculate a number of analyses per unit for each analysis item from the history information, a classification/storage unit configured to classify and store the number of analyses per unit by day of the week, a reagent remaining amount detection unit configured to detect a reagent remaining amount, a conversion unit configured to read each number of analyses per unit over a predetermined period corresponding to a day of a week of an analysis implementation date from the classification/storage unit and convert the read each number of analyses per unit into a reagent usage amount, and a display device configured to arrange and display information associated with the reagent usage amount and the reagent remaining amount for each reagent.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

Provided is a blood collection container that can suppress miRNA leakage and the release of extracellular vesicles during storage after blood collection.　The blood collection container according to the present invention comprises: a blood collection container body; a plasma separation material accommodated in the blood collection container body; and an aqueous solution accommodated in the blood collection container body. The aqueous solution contains an anticoagulant and an antioxidant.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres

Abrégé

The present invention is a shape evaluation method for the first derivative curve F(i) of the blood coagulation reaction. This method comprises the following steps (1) and (2): (1) determining Ts(Xk) and Te(Xk) (k = 0), where Ts(Xk) is the maximum value that satisfies Ts(Xk) < VmaxT, F(Ts(Xk)-d) ≤ B(Xk), and F(Ts(Xk)) ≥ B(Xk); Te(Xk) is the minimum value that satisfies Te(Xk) > VmaxT, F(Te(Xk)) ≥ B(Xk), and F(Te(Xk) + d) ≤ B(Xk), where Xk is 0.5-20; and (2) determining Ts(Xk) and Te(Xk) (k = 1), where Ts(Xk) is the minimum value that satisfies Ts(Xk-1) ≤ Ts(Xk) < VmaxT, F(Ts(Xk) - d) ≤ B(Xk), and F(Ts(Xk)) ≥ B(Xk); Te(Xk) is the maximum value that satisfies VmaxT < Te(Xk) ≤ Te(Xk-1), F(Te(Xk)) ≥ B(Xk), and F(Te(Xk) + d) ≤ B(Xk), where 1 ≤ Xk < 100, and Xk-1 < Xk, B(Xk) = Vmax × Xk %, Vmax is the maximum value of F(i), F(i) = Vmax = F(VmaxT), and d is 0.01-30.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

To provide a means capable of simultaneously and accurately analyzing a component derived from a carrier for liquid phase peptide synthesis with, for example, a target peptide. To provide a means capable of simultaneously and accurately analyzing a component derived from a carrier for liquid phase peptide synthesis with, for example, a target peptide. A method for simultaneously analyzing a carrier for liquid phase peptide synthesis, a component derived from the carrier for liquid phase peptide synthesis, an amino acid to which the carrier for liquid phase peptide synthesis is bound, and a peptide compound to which the carrier for liquid phase peptide synthesis is bound, with a target peptide or final target peptide, the analysis method using high-performance liquid chromatography or supercritical fluid chromatography using an alcohol as an eluent.

Classes IPC  ?

• G01N 30/26 - Conditionnement du fluide vecteurModèles d'écoulement
• B01D 15/32 - Chromatographie en phase liée, p. ex. avec une phase normale liée, une phase inverse ou une interaction hydrophobe
• B01D 15/40 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation utilisant un fluide supercritique comme phase mobile ou comme éluant
• B01D 15/42 - Adsorption sélective, p. ex. chromatographie caractérisée par le mode de développement, p. ex. par déplacement ou par élution
• C07K 1/20 - Chromatographie de partage, de phase inverse ou d'interaction hydrophobe
• G01N 30/02 - Chromatographie sur colonne
• G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes

Abrégé

An object is to provide a diluent for a calibration sample and a sample that is less likely to cause a carryover of the TARC antigen. The object can be solved by a composition including (A) TARC (Thymus and activation-regulated chemokine) and (B) one or more components selected from the group consisting of acidic amino acids, basic amino acids, acidic polymers having an average molecular weight of 4,000 to 1,200,000, and salts thereof, and being in liquid form.

Classes IPC  ?

• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

This blood analysis device (1) comprises: an introduction part (11) into which a blood sample is introduced; a first reagent holder (12) that stores a first reagent for measuring a first measurement item of the blood sample; a first pump (18) that controls feeding of the first reagent; a second reagent holder (13) that stores a second reagent for measuring a second measurement item of the blood sample; a liquid feeding control mechanism (14) that is connected to the introduction part (11), the first pump (18), and the second reagent holder (13), and that controls feeding of the blood sample and the second reagent; a first pipe (15) which is connected to the liquid feeding control mechanism (14) and into which at least a portion of the blood sample is introduced; a liquid chromatography column (16) that is connected to the first pipe (15); and a flow cell type optical detector (17) that is connected to the liquid chromatography column (16) and that measures the first and second measurement items.

Classes IPC  ?

• G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
• C12Q 1/28 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une peroxydase
• G01N 30/02 - Chromatographie sur colonne
• G01N 30/06 - Préparation
• G01N 30/26 - Conditionnement du fluide vecteurModèles d'écoulement
• G01N 30/34 - Contrôle des paramètres physiques du fluide vecteur de la composition du fluide, p. ex. du gradient
• G01N 30/74 - Détecteurs optiques
• G01N 33/66 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir les sucres du sang, p. ex. le galactose
• G01N 33/72 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir les pigments du sang, p. ex. l'hémoglobine, la bilirubine

Abrégé

A reagent for measuring an anti-galactose-deficienct IgG antibodies contains an alkaline phosphatase labeled lectin.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C12Q 1/42 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une phosphatase
• G01N 33/535 - Production de composés immunochimiques marqués avec un marqueur enzymatique

Abrégé

A method for measuring pulmonary surfactant protein D, comprising: a step in which a sample that contains pulmonary surfactant protein D is brought into contact with an insoluble carrier on which an anti-pulmonary-surfactant-protein-D monoclonal antibody is supported; and a step in which a complex of the pulmonary surfactant protein D and at least two anti-pulmonary-surfactant-protein-D monoclonal antibodies is detected. The insoluble carrier supports only one antibody as the anti-pulmonary-surfactant-protein-D antibody.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
• C07K 17/00 - Peptides fixés sur un support ou immobilisésLeur préparation
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12N 5/20 - Cellules murines, p. ex. cellules de souris un des partenaires de la fusion étant un lymphocyte B
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

Provided is a treatment method for optically detecting, by a heterogeneous method, a lectin-binding analyte in a sample. The treatment method comprises, in order: a step for dispensing a pretreatment solution containing a nonionic surfactant into a liquid retention tank serving as both a reaction tank and a detection tank of a container; a step for dispensing a sample into the liquid retention tank; and a step for dispensing a labeled solution containing labeled lectin into the liquid retention tank.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• C12Q 1/28 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une peroxydase
• C12Q 1/42 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase une phosphatase
• G01N 33/483 - Analyse physique de matériau biologique
• G01N 33/536 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide

Abrégé

An object of the invention is to suppress non-specific reaction in an immunoreaction measurement method. An immunoassay method characterized by conducting immunoreaction in the presence of an anti-C3 antibody in a method for measuring an analyte in a sample immunologically is provided. Moreover, a method for suppressing non-specific reaction in a method for immunologically measuring an analyte in a sample which is characterized by conducting immunoreaction in the presence of an anti-C3 antibody is provided.

Classes IPC  ?

• G01N 33/564 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour complexes immunologiques préexistants ou maladies auto-immunes
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

The present invention provides an immunoassay method for assaying SARS-CoV-2 in a biological sample, including using two types of monoclonal antibodies or antibody fragments thereof that bind to a peptide fragment having 30 or less consecutive amino acids in a nucleocapsid protein of SARS-CoV-2, wherein the two types of monoclonal antibodies or antibody fragments thereof recognize different epitopes

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• C07K 14/005 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de virus
• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN

Abrégé

Provided is a blood collection container capable of suppressing contamination of plasma by white blood cells and components in white blood cells. A blood collection container according to the present invention includes a blood collection container main body, a plasma separation material stored in the blood collection container main body, and an aqueous solution stored in the blood collection container main body, in which a solute contained in the aqueous solution contains an anticoagulant, and a total concentration of the solute in the aqueous solution is 100 mM or more and 450 mM or less, or 1200 mM or more.

Classes IPC  ?

• A61M 1/36 - Autre traitement du sang dans une dérivation du système circulatoire naturel, p. ex. adaptation de la température, irradiation
• A61J 1/05 - Récipients spécialement adaptés à des fins médicales ou pharmaceutiques pour recueillir, stocker ou administrer du sang, du plasma ou des liquides à usage médical

Abrégé

This method for analyzing a blood coagulation reaction includes: (1) acquiring a blood coagulation reaction P(i) of a blood sample, where i is a variable representing a measurement point number or time; (2) acquiring t1 and t2 from P(i), where t1 and t2 both represent a measurement point number or a time, t1=i and t2

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

The present invention provides an immunoassay method for assaying SARS-CoV-2, including a step of contacting a biological sample with a monoclonal antibody or an antibody fragment thereof that binds to a nucleocapsid protein of SARS-COV-2, wherein the monoclonal antibody or the antibody fragment thereof binds to the SARS-CoV-2 treated with a serine protease.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN

Abrégé

kkkkkkkk≤100; b) determining a maximum value of V(i) when converted to a relative value.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

Provided is a method for analyzing the blood coagulation capability of a blood specimen. This method comprises: (1) detecting a coagulation reaction end point Pe in a coagulation reaction curve of a blood specimen that is needed to be detected with respect to the presence of a substance having an activity alternative to a coagulation factor VIII; (2) calculating T(X), in which T(X) represents a measurement point or a time at which the coagulation reaction curve reaches X% of Pe; and X represents a variable greater than 0 and 100 or less; and (3) detecting a blood specimen containing a substance having an activity alternative to coagulation factor VIII on the basis of the T(X).

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

The present invention provides a nucleic acid measurement method for measuring a target nucleic acid separately from a metabolite of the target nucleic acid, the nucleic acid measurement method including: a step for hybridizing a fluorescently labeled probe containing a photocrosslinkable artificial nucleic acid with the target nucleic acid to form a target nucleic acid-fluorescently labeled probe complex; a step for irradiating the target nucleic acid-fluorescently labeled probe complex with ultraviolet light to form a covalent bond between the target nucleic acid and the fluorescently labeled probe, thereby forming a target nucleic acid-fluorescently labeled probe bond body; and a step for analyzing the target nucleic acid-fluorescently labeled probe bond body by high-performance liquid chromatography to detect the peak of the target nucleic acid-fluorescently labeled probe bond body from the fluorescence wavelength emitted by the fluorescently labeled probe. This nucleic acid measurement method makes it possible to accurately measure the target nucleic acid separately from the metabolite thereof with the use of high-performance liquid chromatography.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
• G01N 21/64 - FluorescencePhosphorescence
• G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

An object of the present invention is to increase the reactivity of poly A polymerase with an oligonucleotide or a DNA in which the nucleic acid base at the 3′-end is chemically modified, and then to improve the sensitivity of detection through a PALSAR method or the like. The present invention relates to increasing the reactivity of poly A polymerase with an oligonucleotide or a DNA used for the nucleic acid medicine, in which the nucleic acid base at the 3′-end is chemically modified, and then adjusting the Mn2+ concentration during a poly A polymerase reaction, in a method of detecting the oligonucleotide, for a detection target that is an oligonucleotide or a DNA in which the nucleic acid base at the 3′-end is chemically modified, through steps of addition of poly A to the 3′-end of the oligonucleotide with poly A polymerase, capturing with a capture probe, and amplification by a PALSAR method or the like. Thus, the problem of the present invention is solved.

Classes IPC  ?

• C12Q 1/682 - Amplification du signal
• C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]

Abrégé

Provided is a blood separation composition with which a partition wall can be satisfactorily formed even when the blood separation composition is stored for a long time. A blood separation composition according to the present invention contains an organic component having fluidity at 25° C. and fine powder silica, the fine powder silica including hydrophobic silica having a hydrophobicity of 60% or more as measured by a methanol wettability method.

Classes IPC  ?

• A61B 5/15 - Dispositifs de prélèvement d'échantillons de sang
• B01D 21/26 - Séparation du sédiment avec emploi de la force centrifuge
• G01N 1/40 - Concentration des échantillons

Abrégé

Provided is a method for measuring an anti-drug antibody that can be performed more simply and inexpensively than conventional methods. Provided is a double antigen bridging immunoassay using a capture nucleic acid and a tracer nucleic acid. By using the capture nucleic acid and the tracer nucleic acid in the double antigen bridging immunoassay, an anti-drug antibody can be measured simply and inexpensively. Furthermore, by using the tracer nucleic acid, it becomes possible to adopt a high sensitivity detection method utilizing a nucleic acid.

Classes IPC  ?

• C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

The purpose of the present invention is to provide a method for synthesizing a peptide or a long-chain peptide that uses amino acids including an amino acid containing a certain number of hydrophobic amino acids and an amino acid with a functional group on a side chain, the method not including the addition of additional solvents and not reducing the efficiency of Fmoc amino acid condensation reactions or Fmoc removal reactions.　Provided is a method for synthesizing a peptide, the peptide containing one or more amino acids with a functional group on a side chain, 50% or more of a hydrophobic amino acid, and 10% or less of an amino acid in which an α amino group is a secondary amino group; or a peptide with 21 or more residues and containing one or more amino acids with a functional group on a side chain. The method comprises introducing a carrier for peptide synthesis expressed by the general formula (1) below into the side chain of the amino acid with the functional group on a side chain.

Classes IPC  ?

• C07K 1/06 - Procédés généraux de préparation de peptides utilisant des groupes protecteurs ou des agents d'activation

Abrégé

The problem addressed by the present invention is to provide an immunological assay method using a monoclonal antibody and a kit including the monoclonal antibody, which can be used for assaying ICTP without requiring special facilities. This problem can be solved by a method for immunological assay of type I collagen C-terminal telopeptide in a biological sample, including contacting type I collagen C-terminal telopeptide with a monoclonal antibody that recognizes an amino acid sequence represented by GFDFSFLP (SEQ ID NO: 1) as an epitope.

Classes IPC  ?

• G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

This blood coagulation time shortening agent for a blood specimen deficient in coagulation factor XII for activated partial thromboplastin time measurement uses, as an active ingredient, a polymer having 2-methacryloyloxyethyl phosphorylcholine as a structural unit.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang
• A61K 31/198 - Alpha-amino-acides, p. ex. alanine ou acide édétique [EDTA]
• A61K 31/785 - Polymères contenant de l'azote
• A61K 33/00 - Préparations médicinales contenant des ingrédients actifs inorganiques
• A61K 33/06 - Aluminium, calcium ou magnésiumLeurs composés
• A61K 33/26 - FerSes composés
• A61K 33/34 - CuivreSes composés
• A61P 7/04 - AntihémorragiquesProfacteurs de coagulationAgents hémostatiquesAgents antifibrinolytiques
• A61P 43/00 - Médicaments pour des utilisations spécifiques, non prévus dans les groupes
• C08F 20/36 - Esters contenant de l'azote contenant de l'oxygène en plus de l'oxygène de la fonction carboxyle

Abrégé

This blood coagulation time regulator for a blood specimen deficient in coagulation factor VIII, factor IX or factor XI in activated partial thromboplastin time measurement uses, as an active ingredient, a polymer having 2-methacryloyloxyethyl phosphorylcholine as a structural unit. This reagent for activated partial thromboplastin time measurement contains an amino acid and a polymer having 2-methacryloyloxyethyl phosphorylcholine as a structural unit.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

This reagent for measuring activated partial thromboplastin time contains a polymer having 2-methacryloyloxyethyl phosphorylcholine as a structural unit. This blood coagulation time regulator for a lupus anticoagulant-positive blood specimen or a heparin-containing blood specimen in activated partial thromboplastin time measurement uses, as an active ingredient, a polymer having 2-methacryloyloxyethyl phosphorylcholine as a structural unit.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang
• A61P 7/00 - Médicaments pour le traitement des troubles du sang ou du fluide extracellulaire
• A61K 31/80 - Polymères contenant des hétéro-atomes non prévus par les groupes

Abrégé

Provided is a non-specific reaction inhibitor which comprises a copolymer having a constituent unit A derived from 2-methacryroyloxyethyl phosphorylcholine and exhibiting a specific property X measured by Fourier transform infrared spectroscopy employing an attenuated total reflection (ATR) method, and which is used for inhibiting the occurrence of a non-specific reaction when an antibody or an antigen contained in a biological sample is measured. The copolymer is mixed with the biological sample upon use.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques
• C08F 220/10 - Esters
• C08F 230/02 - Copolymères de composés contenant un ou plusieurs radicaux aliphatiques non saturés, chaque radical ne contenant qu'une seule liaison double carbone-carbone et contenant du phosphore, du sélénium, du tellure ou un métal contenant du phosphore
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/542 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec inhibition stérique ou modification du signal, p. ex. extinction de fluorescence
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

Provided is a blood storage composition which can prevent the contamination of a plasma layer with DNA derived from blood cells, and can prevent, compared to the amount of a plasma before storage, a decrease in the amount of a plasma after storage.　A blood storage composition according to the present invention comprises: an anticoagulant (A); a compound (B) that is a disaccharide, a disaccharide derivative, a polysaccharide or a polysaccharide derivative; and a polyether compound (C) that is a polyethylene oxide or polyethylene glycol.

Classes IPC  ?

• A61K 35/14 - SangSang artificiel
• A61P 7/02 - Agents antithrombotiquesAnticoagulantsAnti-agrégants plaquettaires
• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN

Abrégé

Provided is an industrially useful method for producing an alkylsilyloxy-substituted benzylamine compound, in which impurities are easily removed and the method does not involve an alkylsilyloxy-substituted benzyl compound that is an intermediate compound that is unstable to acids.　The present invention relates to a method for producing a ketimine compound represented by general formula (3) (in the formula, R1bto R5bBB are as defined below), the method being characterized by reacting a silazane compound with a benzoyl compound represented by general formula (2) (in the formula, 1 to 5 of R1bto R5bBB is a hydrogen atom, a hydroxyl group, an alkoxy group having 1-6 carbon atoms, or the like).

Classes IPC  ?

• C07C 249/02 - Préparation de composés contenant des atomes d'azote, liés par des liaisons doubles à un squelette carboné de composés contenant des groupes imino
• C07C 251/16 - Composés contenant des atomes d'azote, liés par des liaisons doubles à un squelette carboné contenant des groupes imino ayant des atomes de carbone de groupes imino liés à des atomes d'hydrogène ou à des atomes de carbone acycliques à des atomes de carbone d'un squelette carboné non saturé contenant des cycles aromatiques à six chaînons
• C07C 269/04 - Préparation de dérivés d'acide carbamique, c.-à-d. de composés contenant l'un des groupes l'atome d'azote ne faisant pas partie de groupes nitro ou nitroso à partir d'amines avec formation de groupes carbamate
• C07C 271/12 - Esters des acides carbamiques ayant des atomes d'oxygène de groupes carbamate liés à des atomes de carbone acycliques avec les atomes d'azote des groupes carbamate liés à des atomes d'hydrogène ou à des atomes de carbone acycliques à des atomes d'hydrogène ou à des atomes de carbone de radicaux hydrocarbonés non substitués
• C07B 61/00 - Autres procédés généraux
• C07F 7/18 - Composés comportant une ou plusieurs liaisons C—Si ainsi qu'une ou plusieurs liaisons C—O—Si

Abrégé

Provided is an oligonucleotide measurement method that is simpler and has higher sensitivity and superior specificity and quantitative properties compared with conventional measurement methods. Also provided is an oligonucleotide measurement method having superior specificity which makes it possible to distinguish an intact target oligonucleotide (unmodified form) from a metabolite thereof so as to detect only the unmodified form.　In a hybridization method using a capture probe and an assist probe, a capture probe and an assist probe each having a short base length within a certain range, and in particular, an assist probe having a short base length within a certain range that is not generally considered, are used, and a defective nucleotide site in a metabolite of a nucleic acid drug and the assist probe are hybridized in a specific positional relationship. As a result, a target oligonucleotide in a sample can be detected, and the target oligonucleotide and a metabolite of the nucleic acid drug can be distinguished from each other.

Classes IPC  ?

• C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
• C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes

Abrégé

A method for estimating a cause of coagulation time prolongation includes 1) detecting a coagulation reaction end point Pe in a coagulation reaction curve of a subject blood specimen having a prolonged coagulation time; 2) calculating T(X), wherein T(X) represents a measurement point or time at which the coagulation reaction curve reaches X % of Pe, and X is a variable of greater than 0 and equal to or less than 100; and 3) estimating a cause of coagulation time prolongation of the subject blood specimen based on a form of T(X).

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

An immunoassay method, which is for immunologically assaying a target substance to be measured in a sample, characterized by comprising performing an immunoreaction in the presence of anti-immunoglobulin L-chain lambda monoclonal antibody and anti-immunoglobulin L-chain kappa monoclonal antibody, or in the presence of anti-immunoglobulin L-chain lambda monoclonal antibody, or in the presence of anti-immunoglobulin L-chain kappa monoclonal antibody. This immunoassay method enables suppression of a non-specific reaction that cannot be resolved by conventional non-specific reaction suppressing agents.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

Provided is a blood coagulation reaction analysis method. The method includes measuring a blood coagulation reaction of a subject specimen and acquiring first data for calculating a blood coagulation time of the subject specimen and second data for estimating a blood coagulation abnormality factor of the subject specimen, wherein the acquiring of the second data includes: obtaining a first derivative V(i) of a coagulation reaction curve R(i); and determining a point pk where V(i) assumes Xk before reaching a maximum value of V(i), Vmax, and a point qk where V(i) assumes Xk after reaching Vmax.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

A temperature adjustment system includes: a temperature adjustment unit for adjusting a temperature of a liquid required for measurement to a desired temperature; a measurement unit for obtaining measurement information of a measurement object-containing liquid; a connection flow path connecting the temperature adjustment unit and the measurement unit; temperature detection units and to detect temperatures of the liquids in the temperature adjustment unit and the measurement unit; and a control unit to perform liquid temperature control to control a temperature of the temperature adjustment unit such that the temperature of the liquid in the measurement unit becomes a target temperature while considering a temperature change associated with a flow of the liquids from the temperature adjustment unit to the measurement unit through the connection flow path, based on the temperature of the liquid in the measurement unit and the temperature of the liquid in the temperature adjustment unit.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

Provided is a nucleic acid purification method capable of efficiently purifying a nucleic acid. The nucleic acid purification method including the steps of: mixing a nucleic acid with an antifoaming agent and a coprecipitation agent; and purifying the nucleic acid, in which the antifoaming agent is at least one of a nonionic surfactant and a silicone antifoaming agent.

Classes IPC  ?

• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN

Abrégé

The present invention addresses the problem of suppressing non-specific reactions in an immunological assay method.　Provided is an immunological assay method for immunologically measuring a measurement substance in a sample, the immunological assay method being characterized in that an immunoreaction is carried out in the presence of an anti-C1 antibody or an anti-C2 antibody. Also provided is a method for suppressing non-specific reactions in a method for immunologically measuring a measurement substance in a sample, the method for suppressing non-specific reactions being characterized in that an immunoreaction is carried out in the presence of an anti-C1 antibody or an anti-C2 antibody.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Provided is a qualitative or quantitative test method for detection target substances contained in a specimen, said test method comprising a preparation step for preparing a test sample from the specimen and a test step for testing at least part of the test sample with a reagent for detecting each of a first detection target substance and a second detection target substance, wherein: the first detection target substance has an isoelectric point of not less than 10.0; and, in the preparation step and/or the test step, the first detection target substance is brought into contact with a member made of glass and/or a member made of nitrocellulose, in the presence of a cationic substance. This test method makes it possible to use the same specimen to detect, with high sensitivity, two or more detection target substances, which include a substance with an isoelectric point of not less than 10.0, while suppressing a reduction in measurement sensitivity.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

A method for measuring human cells, wherein the method includes a preparation step that prepares a sample containing human cells and non-human animal components derived from non-human animal cells, a lysis step that lyses the cell membranes in the sample, and an assay step that performs hybridization assay to a nucleic acid sequence specific to the human cells. The method for measuring human cells does not require correction of the recovery rate by genomic DNA extraction or correction of the amount of genomic DNA by quantification of an internal standard, etc.

Classes IPC  ?

• C12Q 1/6813 - Tests d’hybridation
• C12Q 1/06 - Détermination quantitative
• C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées

Abrégé

A method for detecting a blood coagulation reaction, comprising: 1) measuring a blood coagulation reaction of a subject blood specimen and acquiring a first derivative V(i) of a coagulation reaction up to a latest measurement point; 2) calculating areas under the curve (AUCs) before the peak and after the peak, wherein a top of the peak of V(i) is cVmax(k) which is a maximum value of V(i); and 3) detecting cVmax(k) as Vmax which is a true maximum value of V(i) when the pre-peak AUC and the post-peak AUC are both equal to or higher than a first threshold AUCth1 and a period L during which the pre-peak AUC and the post-peak AUC have continued to be a constant value reaches a predetermined length.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

An object of the present invention is to provide a method for measuring an object to be measured in a specimen by an enzymatic method, the measurement method being able to suppress the positive influence of peroxide derived from the specimen. More specifically, an object of the present invention is to provide a measurement method and a measurement reagent that can suppress elevation in value regardless of whether or not the specimen is a catalase-free specimen. Provided is a measurement method that can accurately quantify hydrogen peroxide derived from an object to be measured, without influence derived from a specimen, by contacting the specimen with an enzyme in the presence of at least one compound selected from the group consisting of a compound represented by the following general formula (I), a benzimidazole derivative having an electron-donating substituent at position 2, and histidine, wherein R1 and R2 are the same or different and each represent hydrogen, a linear or branched alkyl group having 1 to 6 carbon atoms and optionally having a substituent, an aryl group optionally having a substituent, or an alkyloxy group having 1 to 6 carbon atoms. An object of the present invention is to provide a method for measuring an object to be measured in a specimen by an enzymatic method, the measurement method being able to suppress the positive influence of peroxide derived from the specimen. More specifically, an object of the present invention is to provide a measurement method and a measurement reagent that can suppress elevation in value regardless of whether or not the specimen is a catalase-free specimen. Provided is a measurement method that can accurately quantify hydrogen peroxide derived from an object to be measured, without influence derived from a specimen, by contacting the specimen with an enzyme in the presence of at least one compound selected from the group consisting of a compound represented by the following general formula (I), a benzimidazole derivative having an electron-donating substituent at position 2, and histidine, wherein R1 and R2 are the same or different and each represent hydrogen, a linear or branched alkyl group having 1 to 6 carbon atoms and optionally having a substituent, an aryl group optionally having a substituent, or an alkyloxy group having 1 to 6 carbon atoms.

Classes IPC  ?

• G01N 33/72 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir les pigments du sang, p. ex. l'hémoglobine, la bilirubine
• C12P 3/00 - Préparation d'éléments ou de composés inorganiques à l'exception du dioxyde de carbone
• C07C 49/86 - Cétones comportant un groupe cétone lié à un cycle aromatique à six chaînons contenant des groupes —CHO

Abrégé

Provided is a blood analysis method including: acquiring coagulation reaction data on a blood specimen; calculating a parameter related to a centroid point from a differential curve of the coagulation reaction data; and evaluating coagulation properties of the blood specimen using the parameter related to the centroid point.

Classes IPC  ?

• G01N 33/49 - Analyse physique de matériau biologique de matériau biologique liquide de sang

Abrégé

Provided is a method for measuring an anti-drug antibody with ultra-high sensitivity, easily and at low cost compared to conventional methods.　Provided is a method for measuring an analyte with ultra-high sensitivity, by using a capture probe and an assist probe and employing an improved PALSAR method. According to the present invention, by using a capture probe and an assist probe in the double antigen bridging immunoassay and employing an improved PALSAR method, an anti-drug antibody can be measured with ultra-high sensitivity, easily and at low cost.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet

Abrégé

Provided is a blood coagulation time measurement method. In the method, reaction X(i) is acquired through smoothing and zero-point adjustment of a measured value P(i) for coagulation reaction of a blood specimen, and then an integration ratio Z(i) of the reaction X(i) is acquired. These values are used to calculate an index for coagulation time Tc calculation, and it is determined whether or not the index satisfies the criteria. The procedure is sequentially repeated until an index that satisfies the criteria is obtained.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang
• G01N 21/77 - Systèmes dans lesquels le matériau est soumis à une réaction chimique, le progrès ou le résultat de la réaction étant analysé en observant l'effet sur un réactif chimique

Abrégé

An object of the present invention is to provide a more sensitive detection method and quantification method in a method for detecting a target nucleic acid using the PALSAR method. The present invention provides a method for recovering a target nucleic acid in a sample, comprising: (i) a step of bringing a target nucleic acid in a sample, a capture probe, and an assist probe into contact with each other for hybridization, the capture probe containing (A) a nucleic acid probe, and (B) a solid phase or an adapter or linker flanking to a 3′-end or 5′-end nucleotide of the nucleic acid probe, the nucleic acid probe containing: a first base sequence; and a second base sequence complementary to a complete or partial sequence of the target nucleic acid, the assist probe containing a 6- to 9-mer sequence capable of flanking to the target nucleic acid and complementary to a complete or partial sequence of the first base sequence of the nucleic acid probe; and (ii) a step of recovering a hybridization product contained in the sample.

Classes IPC  ?

• C12Q 1/682 - Amplification du signal

Abrégé

Provided is an inspection chip with simplified channel switching structure, which is generally complicated. An inspection chip 1 including a chip main body 2 having a specimen introduction channel, an adsorption channel including an adsorption unit, a first waste liquid channel, a recovery liquid introduction channel, and a detection channel including a detection unit; and a rotary valve 3 attached to the chip main body 2 so as to be rotatable about a rotation axis, the rotary valve 3 having a plurality of connection channels, the plurality of connection channels being arranged so that the rotary valve 3 is capable of taking at least a first state and a second state when the rotary valve 3 rotates about the rotation axis, the first state being a state in which the specimen introduction channel, the adsorption channel, and the first waste liquid channel are connected so as to be provided in this order from an upstream side, and the second state being a state in which the recovery liquid introduction channel, the adsorption channel, and the detection channel are connected so as to be provided in this order from the upstream side.

Classes IPC  ?

• G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
• G01N 30/26 - Conditionnement du fluide vecteurModèles d'écoulement

Abrégé

In a sample treatment system (10), reagent information pertaining to a reagent, which includes position information pertaining to the reagent, is stored for each reagent in a reagent information database (72). Then, on the basis of the position information pertaining to the reagent which is included in the reagent information, a display control unit (90) causes a touch display (82) of a terminal (14) to display a reagent information display screen (91) that includes a reagent recognition mark display area (91A) which displays a reagent recognition mark (92) corresponding to the reagent so as to correspond to the placement position of the reagent placed in a reagent cooling box (60).

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
• G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse

Abrégé

[Problem] To obtain an automatic analysis device that can efficiently perform a coagulation measurement and a measurement differing from the coagulation measurement. [Solution] The present invention comprises: a recording part in which are recorded one or more measurement items that are performed with respect to a sample placed in at least one sample container, and the measurement order for each measurement item; a sample dispensation mechanism 50 which dispenses, into a first reaction container and/or a second reaction container, a sample that has been selected for measurement on the basis of the measurement order; a first measurement part 25 which, after a prescribed first preparation process has been completed, performs measurement for a coagulation item with respect the sample that has been dispensed into the first reaction container; and a second measurement part 35 that, after a prescribed second preparation process has been completed, performs a specific measurement that is a measurement item differing from the measurement for the coagulation item, with respect to the sample that has been dispensed into the second reaction container. When the first preparation process has been completed, the first measurement part is capable of performing the measurement for the coagulation item, regardless of whether or not the second preparation process has been completed.

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

According to the present invention, a coagulation time extension factor estimation method involves 1) acquiring Tm(X) and Tn(X) and 2) estimating a coagulation time extension factor for a tested blood sample on the basis of Tm(X). Tm(X) represents a measurement point or time at which a coagulation reaction curve for sample M reaches X% of a coagulation reaction end point Em, and Tn(X) represents a measurement point or time at which a coagulation reaction curve for sample N reaches X% of a coagulation reaction end point En. Em is the coagulation reaction end point for the coagulation reaction curve for sample M, and En is the coagulation reaction end point for the coagulation reaction curve for sample N. Sample S is a tested blood sample that has an extended coagulation time, sample N is a normal blood sample, and sample M is a mixed sample of sample S and sample N. X is greater than 0 but no greater than 100.

Classes IPC  ?

• G01N 33/86 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir le temps de coagulation du sang

Abrégé

The present invention addresses the problem of providing a technology for increasing the sensitivity of a latex immunoagglutination reagent while suppressing sedimentation of particles in a latex particle dispersion liquid. In particular, the present invention addresses the problem of providing a latex particle dispersion liquid that can be applied to a latex immunoagglutination reagent having sufficient sensitivity while suppressing temporal generation of aggregates of large-size particles in the latex particle dispersion liquid.　Provided is a latex particle dispersion liquid in which an aqueous medium is used as a dispersion medium, and which contains at least one compound selected from the group consisting of polyacrylic acid, sodium polyacrylate, polyoxyethylene oxide, and sodium poly-γ-glutamate. The present invention also provides an immunoagglutination reagent to which the latex particle dispersion liquid is applied.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/545 - Résine synthétique

Abrégé

A specimen processing system (10) in which a quality control measurement information database (74) stores a plurality of quality control data, which constitute time series data of quality control values obtained by analyzing a quality control sample by using an analysis unit (22). A display control unit (88) simultaneously displays quality control data for a plurality of quality control samples which are of the same type but different manufacturing lot numbers on a touchscreen display (80), on the basis of the quality control data stored in the quality control measurement information database (74).

Classes IPC  ?

• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet

Abrégé

Provided is a kit for isolating circulating tumor cells, the kit being capable of increasing the recovery rate of circulating tumor cells, not only when a specimen, which has been stored for not a long period of time after collection, is used, but also when a specimen, which has been stored for several days after the collection of blood, is used.　The kit for isolating circulating tumor cells according to the present invention is used for isolating circulating tumor cells in the blood, and comprises a blood collection container in which an aqueous solution is contained and a predetermined amount of blood is collected, and a cell isolation container in which a cell isolation material having a specific gravity of 1.065-1.080 at 25 °C is contained, wherein: the aqueous solution contains an anticoagulant, and a low-molecular compound having a molecular weight of 75-500 or a high-molecular compound having a number-average molecular weight of 2,000-200,000 (exclusive of 200,000); and when a physiological saline, of an equal amount as a predetermined amount of the blood collected in the blood collection container, is collected into the blood collection container and a mixed solution, in which the physiological saline and the aqueous solution are mixed, is obtained, the osmotic pressure of the mixed solution is 270-350 mOsm/L.

Classes IPC  ?

• G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12N 1/02 - Séparation des micro-organismes de leurs milieux de culture
• C12N 5/09 - Cellules tumorales

Abrégé

The present invention provides an immunological detection method for a trimeric type-I collagen N-terminal propeptide in a biological sample. The immunological detection method uses a first antibody that binds to a specific portion of a pro-α1-chain in the trimeric type-I collagen N-terminal propeptide, and a second antibody that binds to a specific portion of a pro-α2-chain in the trimeric type-I collagen N-terminal propeptide. The immunological detection method can be performed easily, and can specifically (selectively) measure, in a biological sample containing monomers and trimers of an α-chain of PINP, the trimers.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains

Abrégé

The present invention provides a method for immunologically detecting a trimeric type 1 collagen N-terminal propeptide in a biological sample, said immunological detection method comprising using a first antibody that binds to a first specific portion of the pro-α1 chain in the trimeric type I collagen N-terminal propeptide and a second antibody that binds to a second specific portion of the pro-α1 chain in the trimeric type I collagen N-terminal propeptide. The immunological detection method is easy to handle and enables specific (selective) measurement of the trimer in a biological sample containing the trimer and monomer of the α-chain of PINP.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains

Abrégé

Provided is an immunological analysis method comprising a step for binding an antibody to an antigen, wherein the antibody is included in a complex which includes a target substance and a structure that is obtained by binding a plurality of antibodies together with a scaffold compound therebetween.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/483 - Analyse physique de matériau biologique

Abrégé

An immunoassay reagent that contains a substance exhibiting an immunoreaction and insoluble carriers capable of binding to the aforesaid substance, wherein the insoluble carriers include an insoluble carrier to which the substance is bound and an insoluble carrier to which the substance is not bound.

Classes IPC  ?

• G01N 33/531 - Production de matériaux de tests immunochimiques

Abrégé

Provided is an oligonucleotide measurement method, which is simpler and has higher sensitivity and superior specificity and quantitatively compared with the conventional measurement methods. Also provided is an oligonucleotide measurement method having excellent specificity, whereby it becomes possible to distinguish an intact target oligonucleotide (a non-changed form) and a metabolite thereof from each other and detect only the non-changed form.　In a hybridization method using a capture probe and an assist probe, a capture probe and an assist probe each having a short nucleotide length falling within a specified range are used and a defective nucleotide site in a metabolite of an oligonucleotide therapeutic and the capture probe are hybridized with each other in a specific positional relationship. As a result, a target oligonucleotide in a sample can be detected, and the target oligonucleotide and a metabolite of the oligonucleotide therapeutic can also be distinguished from each other.

Classes IPC  ?

• C12Q 1/6813 - Tests d’hybridation
• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes

Abrégé

Provided is an oligonucleotide measurement method performed in a simple manner, with higher sensitivity and superior specificity and quantitativity, as compared with conventional measurement methods. Also provided is an oligonucleotide measurement method with superior specificity, which makes it possible to distinguish an intact target oligonucleotide (unmodified form) from a metabolite thereof so as to detect only the unmodified form.　In a hybridization method using a capture probe and an assist probe, as a result of inserting a spacer between a solid phase and a nucleic acid probe, which are included in the capture probe, it becomes possible to detect a target oligonucleotide in a sample as a matter of course, but also to distinguish said oligonucleotide from a metabolite of a nucleic acid drug.

Classes IPC  ?

• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12Q 1/6813 - Tests d’hybridation
• C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
• C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes