A bioprocessing system includes a process vessel, a plurality of filter housings, a feed pump for moving fluid between the process vessel and the filtration assembly, and a retentate valve within a retentate line. The retentate valve includes a fluid passageway to selectively direct movement of the fluid to at least one of the plurality of filter housings. A permeate valve is provided in a permeate line between the filter housings and a permeate pump. A pressure sensor is provided in the permeate line, and a controller is coupled to the pressure sensor, the retentate valve, and the permeate valve. The controller actuates the retentate and permeate valves to direct flow between the process vessel one or more of the filter housings, and to direct permeate flow from the filter housings to the permeate pump based on information received from the flow sensor and the pressure sensor.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
Embodiments of the present disclosure include a device for stirring that includes a magnetic material. The magnetic material is disposed within a housing (210), and the housing includes a cup and a cover. The housing encapsulates the magnetic material, and the magnetic material is non-rotatable within the housing. The device also includes an agitator (230) connected to at least a portion of the housing, the agitator including a sleeve and a plurality of vanes. The housing, magnetic material, and agitator are configured to selectively rotate about an axis of rotation. Embodiments of the present disclosure include a method for manufacturing a device for stirring. The method includes encapsulating a magnetic material in a housing, the housing including a cup and a cover. The method includes joining the cup and the cover with a mating connection. The method also includes overmolding an agitator over at least a portion of the housing, where the agitator covers the mating connection.
Provided herein are affinity agents comprising ligands that specifically bind target molecules. The affinity agents are useful for binding, isolation, and/or purifications.
C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
The technology provides systems and methods to isolate a biologic product from a process fluid by performing tangential flow filtration or a tangential flow depth filtration. The technology performs the filtration by drawing or pumping the fluid downward through the filter and controlling the flow rate such that a consistent pressure is maintained along the length of the filter.
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
C07K 14/005 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de virus
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
A61K 38/00 - Préparations médicinales contenant des peptides
Provided are kappa light chain (KLC)-binding proteins and related compositions and methods. The KLC-binding proteins have a structure of Formula I: LN1231nn-LC22 fragment, or a diabody or triabody.
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
7.
SYSTEMS AND METHODS FOR TANGENTIAL FLOW DEPTH FILTRATION
The present disclosure relates to hollow fiber tangential flow filter units, including hollow fiber tangential flow depth filter units, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.
B01D 63/04 - Modules à fibres creuses comprenant plusieurs ensembles à fibres creuses
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
A serpentine co-flow fluid filtration system includes two or more filter housings including filter elements, a pump to transfer fluid from a vessel to the filter housings, and a permeate recirculation pump to transfer permeate back to each filter housing. The system includes a control system programmed to transfer the fluid from the vessel to the first filter housing, selectively remove permeate from the first filter housing, and selectively recirculate permeate to a second filter housing. The permeate in each filter housing is provided at a flow rate and pressure such that a difference between a pressure of the fluid and the permeate at an entrance to the filter housing is equal to a difference between a pressure of the fluid and the permeate at an exit of the filter housing. The system allows for additional filters to be connected in series.
Provided are affinity ligands that bind to Hepatitis B surface antigen (HBsAg), affinity resins comprising the HBsAg-binding ligands, and related compositions and methods for the isolation and/or purification of HBsAg.
A method includes determining whether a variation in probe radiation intensity meets a stability criterion; directing the probe radiation through a probe, when the probe is disposed at a first position, defining a first path length L1 of the probe radiation through the fluid sample; measuring a transmitted intensity I1 of the probe radiation after passing through the fluid sample when the probe is disposed at the first position; directing the probe radiation through the probe when the probe is disposed at a second position, defining a second path length L2 of the probe radiation through the fluid sample; measuring a transmitted intensity I2 of the probe radiation after passing through the fluid sample when the probe is disposed at the second position; and determining a concentration C of a material in the fluid sample based upon L1, I1, L2, and I2, when the stability criterion is met.
G01J 3/42 - Spectrométrie d'absorptionSpectrométrie à double faisceauSpectrométrie par scintillementSpectrométrie par réflexion
G01J 3/10 - Aménagements de sources lumineuses spécialement adaptées à la spectrométrie ou à la colorimétrie
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. A permeate recirculation loop is provided to recirculate permeate flow within the filter housing in the same direction as the fluid being filtered.
A device (200) for measuring light absorbance of a sample (208) includes a light guiding hollow fiber (202) having proximal and distal ends (202a, 202b) and an axial bore (202c), and a solid optical fiber (204) having proximal and distal ends (204a, 204b), where the solid optical fiber (204) is axially movable within the axial bore (202c) of the light guiding hollow fiber (202). A light source (102) is operably coupled to the solid optical fiber probe (204). A sample holder (206) has a bottom surface, and the distal end (202b) of the light guiding hollow fiber (202) is engaged with the bottom surface. An optical detector (210) is aligned with the distal end (202b) of the light guiding hollow fiber (202) for receiving light therefrom. A method of using the device.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
G01N 21/85 - Analyse des fluides ou solides granulés en mouvement
14.
FILTRATION SYSTEM WITH OPTIMIZED RETENTATE/PERMEATE FLOW SEQUENCING
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a valve disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the valve is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction. A controller controls the pump and valve to achieve a predetermined stroke profile during each of the first and second modes of operation.
A system may include a light source, to generate a probe signal, comprising incident radiation; an optical probe, to direct the incident radiation through a fluid sample; a motor, to move the optical probe along a probe axis, to change a path length of the incident radiation through the fluid sample; and a detector, to receive the incident radiation as attenuated radiation after passing through the fluid sample. The system may include a control system, arranged to: initiate an absorbance measurement by directing movement of the optical probe along the probe axis; direct the light source to emit the incident radiation; and automatically adjust at least one measurement parameter of a set of measurement parameters for the sample measurement, based upon a slope parameter m, wherein m is derived from a rate of change in an intensity of the attenuated radiation with a change in the path length.
G01N 21/33 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière ultraviolette
Provided are compositions, systems and methods for removal of double stranded RNA from a mixture utilizing affinity agents having high binding affinity and selectivity for double stranded RNA over single stranded RNA.
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale
F16K 27/02 - Structures des logementsMatériaux utilisés à cet effet des soupapes de levage
F16L 57/00 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes
F16L 57/02 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes contre la fissuration ou le flambement
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Laboratory consumables kits for use with cell culture media analyzers comprised primarily of chemicals in the nature of standard solutions used in analytical chemistry; Chemical products for laboratory, biopharmaceutical processing, and research purposes, namely reference standards for use in spectrometry Optical fibers for use in spectroscopy; Downloadable and recorded and embedded system control software and data analysis software for use in connection with spectrometry for laboratory, biopharmaceutical processing, and research purposes; Measuring apparatus for spectroscopy, namely, a variable path length apparatus and apparatus for measurement of real time solution concentrations, not for medical purposes; Analytical instruments not for medical use for analysis of the compositional analysis of solids, liquids and gases employed in bioprocess development and production using chemical sensors and downloadable computer software and computer hardware with embedded software for operating the foregoing analytical instruments and communicating results and control signals with other systems; Detection and identification instruments using light sources and light detectors for detecting, identifying, and quantifying chemical and biological substances not for medical purposes; Scientific apparatus, namely, spectrometers and parts, fittings, probes, fiberoptic cables thereof; Microfluidic separation plates used for non-medical purposes featuring microscopic grooves used to separate chemical and biological substances; Scientific analytical instruments, namely, bioprocess analyzers as laboratory bench top instruments using mass spectrometry (MS) and microfluidic technology for analyzing chemical and biological substances in order to detect for the presence, absence or quantity of target substances in the nature of chemicals, biologics and contaminants, for purposes of biopharmaceutical research; Laboratory consumables kits for use with cell culture media analyzers comprised primarily of microfluidic chips for upstream and downstream bioprocess analysis, laboratory microplates, and covers for laboratory microplates, and also consisting of background electrolytes for upstream and downstream bioprocess analysis, and diluent for cell culture analysis.
A tubing assembly includes first and second tubing segments, and a pressure cup portion formed between the first and second tubing segments. The pressure cup portion has a first open lateral side, and a second closed lateral side including a flat membrane member configured to engage a diaphragm of a pressure transmitter to transmit a pressure of a fluid disposed within the pressure cup portion to said diaphragm. The flat membrane member is integrally formed with the pressure cup portion. A mold for forming the tubing assembly, and a pressure sensing system includes the tubing assembly, a housing portion, and a pressure sensor receivable within the housing to position a diaphragm of the pressure sensor directly adjacent to the flat membrane member of the tubing assembly.
B29C 45/00 - Moulage par injection, c.-à-d. en forçant un volume déterminé de matière à mouler par une buse d'injection dans un moule ferméAppareils à cet effet
F16L 41/00 - Tuyaux de branchementRaccordements des tuyaux aux parois
F16L 41/02 - Ensembles de branchements, p. ex. d'une seule pièce, soudés à l'autogène, rivetés
G01L 19/00 - Détails ou accessoires des appareils pour la mesure de la pression permanente ou quasi permanente d'un milieu fluent dans la mesure où ces détails ou accessoires ne sont pas particuliers à des types particuliers de manomètres
A tubing assembly includes first and second tubing segments, and a pressure cup portion formed between the first and second tubing segments. The pressure cup portion has a first open lateral side, and a second closed lateral side including a flat membrane member configured to engage a diaphragm of a pressure transmitter to transmit a pressure of a fluid disposed within the pressure cup portion to said diaphragm. The flat membrane member is integrally formed with the pressure cup portion. A mold for forming the tubing assembly, and a pressure sensing system includes the tubing assembly, a housing portion, and a pressure sensor receivable within the housing to position a diaphragm of the pressure sensor directly adjacent to the flat membrane member of the tubing assembly.
G01L 19/00 - Détails ou accessoires des appareils pour la mesure de la pression permanente ou quasi permanente d'un milieu fluent dans la mesure où ces détails ou accessoires ne sont pas particuliers à des types particuliers de manomètres
21.
UNIVERSAL MODULAR PRESSURE SENSORS AND FITTINGS THEREFOR
A system for monitoring pressure in a bioprocessing system includes a binder connector or industrial connector including a plurality of electrically conductive pins disposed therethrough. A sensor cap has an opening for receiving a distal portion of the binder connector. A sensor spacer has an upper end engageable with a surface of the sensor cap. The sensor spacer has an opening for receiving wire leads coupled to the electrically conductive pina. A sensor has a printed circuit board (PCB) disposed thereon. The PCB includes a plurality of wire solder points for electrically coupling to the wire leads. A sensor insert having a sealing portion receives the sensor therein. A sensor housing has a seat portion for receiving the sealing portion thereon and an upper flange portion for coupling to the sensor cap.
G01L 19/00 - Détails ou accessoires des appareils pour la mesure de la pression permanente ou quasi permanente d'un milieu fluent dans la mesure où ces détails ou accessoires ne sont pas particuliers à des types particuliers de manomètres
The disclosure is directed to pneumatic actuator assemblies for reduced dead leg. An assembly includes a valve body (104), and a first actuator (101) and a second actuator (102) each coupled to the valve body by a bonnet assembly (112). The bonnet assembly includes a bonnet body (125) having a first end (126) opposite a second end (128), wherein the first end is connected with the valve body, and a nut (130) threadedly connected to the second end of the bonnet body. The bonnet assembly further includes an actuator bonnet (134), wherein the nut partially surrounds the actuator bonnet, and wherein the actuator bonnet is rotatable about a lengthwise axis (LA1, LA2) extending through a center of a stem (124) of a piston (118).
F16K 7/06 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par tige filetée, came ou autre moyen mécanique
F16K 27/00 - Structures des logementsMatériaux utilisés à cet effet
F16K 27/02 - Structures des logementsMatériaux utilisés à cet effet des soupapes de levage
F16K 31/122 - Moyens de fonctionnementDispositifs de retour à la position de repos actionnés par un fluide le fluide agissant sur un piston
F16K 7/07 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par pression d'un fluide
F16K 11/22 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont plusieurs éléments de fermeture ne se déplacent pas comme un tout actionnés par des organes de commande distincts chacun étant propre à une soupape, p. ex. conjugués pour former clapet à voies multiples
The disclosure is directed to pneumatic actuator assemblies for reduced dead leg. In one approach, an assembly may include a valve body, and a first actuator and a second actuator each coupled to the valve body by a bonnet assembly. The bonnet assembly may include a bonnet body having a first end opposite a second end, wherein the first end is connected with the valve body, and a nut threadedly connected to the second end of the bonnet body. The bonnet assembly may further include an actuator bonnet, wherein the nut partially surrounds the actuator bonnet, and wherein the actuator bonnet is rotatable about a lengthwise axis extending through a center of a stem of a piston.
F16K 31/122 - Moyens de fonctionnementDispositifs de retour à la position de repos actionnés par un fluide le fluide agissant sur un piston
F16K 7/07 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par pression d'un fluide
09 - Appareils et instruments scientifiques et électriques
Produits et services
Downloadable or embedded software used in connection with measuring and analyzing chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants for scientific and industrial research and use; all the aforesaid goods to be used in connection programing and controlling automated production systems used in the pharmaceutical and biopharmaceutical industry.
The present invention relates to Fc binding proteins comprising one or more Fc binding domains wherein at least one domain comprises of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-6 or 21. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
C07K 14/31 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Micrococcaceae (F) provenant de Staphylococcus (G)
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
C07K 16/06 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux provenant de sérum
C07K 17/00 - Peptides fixés sur un support ou immobilisésLeur préparation
The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.
B01D 65/08 - Prévention de l'encrassement de la membrane ou de la polarisation par concentration
B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.
A pinch valve including a main body having a jaw defining a tube cradle for holding a tube, a clamp pivotably coupled to the jaw and rotatable between an open position and a closed position, the clamp having a shank extending therefrom, wherein when the clamp is in the open position the shank extends into the tube cradle such that when the tube is placed into the tube cradle the tube engages the shank and rotates the clamp toward the closed position, and wherein when the clamp is rotated from the closed position to the open position the shank pushes the tube at least partially out of the tube cradle, and a linear actuator coupled to a rear of the main body and including a plunger that can be controllably driven into the tube cradle for compressing the tube against the clamp to restrict fluid flow through the tube.
A61M 39/28 - Moyens de serrage pour l'écrasement des tubes flexibles, p. ex. brides de serrage à rouleaux
F16K 7/04 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale
F16K 7/06 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par tige filetée, came ou autre moyen mécanique
09 - Appareils et instruments scientifiques et électriques
Produits et services
Downloadable software used in connection programing and controlling automated production systems used in the pharmaceutical and biopharmaceutical industry to measure and analyze chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants for scientific and industrial research and use; embedded software used in connection programing and controlling automated production systems used in the pharmaceutical and biopharmaceutical industry to measure and analyze chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants for scientific and industrial research and use
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
B01D 29/11 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants en forme de sac, de cage, de tuyau, de tube, de manchon ou analogue
B01D 29/66 - Rinçage par chasse, p. ex. rafale ascendante d'air à contre-courant
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
B01D 65/02 - Nettoyage ou stérilisation de membranes
Viral vectors for gene therapy can be produced using transfection in cell culture systems. The present disclosures describe cell culture systems that include cell retention devices and other components so that transfection occurs at high values of viable cell density (VCD). The present disclosures also include methods of operating cell culture systems at high values of viable cell density for optimized production of viral vectors.
B01D 65/08 - Prévention de l'encrassement de la membrane ou de la polarisation par concentration
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.
A bioprocessing system includes a process vessel, a plurality of filter housings, a feed pump for moving fluid between the process vessel and the filtration assembly, and a retentate valve within a retentate line. The retentate valve includes a fluid passageway to selectively direct movement of the fluid to at least one of the plurality of filter housings. A permeate valve is provided in a permeate line between the filter housings and a permeate pump. A pressure sensor is provided in the permeate line, and a controller is coupled to the pressure sensor, the retentate valve, and the permeate valve. The controller actuates the retentate and permeate valves to direct flow between the process vessel one or more of the filter housings, and to direct permeate flow from the filter housings to the permeate pump based on information received from the flow sensor and the pressure sensor.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chemical analysis instruments, none of them for medical use, for the analysis of biochemical processes namely for the compositional analysis of liquids employed in bioprocess development and production using enzymatic sensors, electrochemical sensors or a combination thereof; computing devices, data-loggers, data acquisition devices, data storage devices, programmable logic control devices, networking devices and associated embedded software for data management and data analysis in the field of chemical analysis process instrumentation and control, such data management and analysis for operating chemical analytical instruments and communicating results and control signals with other network systems.
39.
GENDERLESS CLAMP AND CLAMP HALF FOR SECURING HYGIENIC FITTINGS
A genderless clamp includes a first clamp portion and a second clamp portion. The first clamp portion has a first end and a second end. The second clamp portion has a first end and a second end. For each clamp portion, the first end has ramped barbs and the second end also has ramped barbs. The first clamp portion mates with the second clamp portion around a junction through which liquid is to flow.
Presented herein are methods and systems for monitoring and/or control of production units used at various stages in development and manufacture of biological products. In particular, in certain embodiments, bio-production monitoring and control technologies described herein utilize mid-infrared (mid-IR) analyzers capable of obtaining mid-IR spectral data from aqueous samples in substantially real-time. Technologies described herein may leverage this mid-IR spectral data to measure sample quality metrics, such as protein content, titer, secondary structure, aggregation, etc. as well as viral and/or nucleic acid properties. Sample quality metrics may, accordingly, be measured in substantially real-time and/or on a continuous basis to assess production quality for, e.g., protein therapeutics and gene-therapy agents, and may be used by control systems for adjustment and/or control of process parameters, thereby improving, among other things, product quality.
The present disclosure relates to diaphragm pumps with one or more filters, e.g., tangential flow filters, membranes, and ultrafiltration membranes, for various applications, including, e.g., bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same. In an aspect, a pump for alternating tangential flow filtration may include a diaphragm. A housing may contain the diaphragm. The housing may have a first end open to a retentate channel of a first membrane. A magnetic member may be coupled to the diaphragm. An electromagnetic field generator may be disposed about the magnetic member.
F04B 43/02 - "Machines", pompes ou installations de pompage ayant des organes de travail flexibles ayant des organes flexibles du genre plat, p. ex. des diaphragmes
F04B 45/04 - Pompes ou installations de pompage, ayant des organes de travail flexibles, spécialement adaptées pour fluides compressibles ayant des organes flexibles du genre plat, p. ex. des diaphragmes
A valve assembly with a multidirectional flow path includes a valve body that includes respective hinged portions defining respective passageways extending through the valve body when in a closed state. A flexible conduit or tubing having a loop portion and a plurality of branches extending from the loop portion is at least partially positioned within the valve body. Portions of the loop that extend beyond the valve body are encapsulated by respective rigid jackets. Multiple valves are situated on the valve body and are used to selectively pinch the loop portion in different pinching configurations to direct fluid to or from the particular branches. The valve assembly may be integrated into a manufacturing or other production process with optional additional jackets used to encapsulate the flexible conduit branches.
F16K 11/22 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont plusieurs éléments de fermeture ne se déplacent pas comme un tout actionnés par des organes de commande distincts chacun étant propre à une soupape, p. ex. conjugués pour former clapet à voies multiples
B29C 45/14 - Moulage par injection, c.-à-d. en forçant un volume déterminé de matière à mouler par une buse d'injection dans un moule ferméAppareils à cet effet en incorporant des parties ou des couches préformées, p. ex. moulage par injection autour d'inserts ou sur des objets à recouvrir
B29C 65/00 - Assemblage d'éléments préformésAppareils à cet effet
F16K 7/06 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par tige filetée, came ou autre moyen mécanique
F16K 7/07 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par pression d'un fluide
F16K 27/00 - Structures des logementsMatériaux utilisés à cet effet
F16K 27/02 - Structures des logementsMatériaux utilisés à cet effet des soupapes de levage
F16B 13/00 - Chevilles ou autres dispositifs fixés à des parois ou autres emplacements par insertion dans des trous préparés à cet effet
A fluid filtration system includes a fluid storage vessel such as a bioreactor, one or more filters housings, and at least one pump coupled between the fluid storage vessel and the filter housing(s). A plurality of valves are used to control flow between the pump and the filter housing. The at least one pump is configured to move fluid from the fluid storage vessel through the filter housing(s), and the valves are configured for selectively directing fluid received from the at least one pump to the first or second end of the filter housing(s). A plunger pump can be used to selectively move flow between first and second ends of a filter housing.
B01D 65/08 - Prévention de l'encrassement de la membrane ou de la polarisation par concentration
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
45.
Fc binding proteins with cysteine in the c-terminal helical region
The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
C07K 14/31 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Micrococcaceae (F) provenant de Staphylococcus (G)
A multilayer polymer film comprising an inner biomaterial contact layer and an outer layer, the outer layer comprising a polymer selected from a polyester elastomer copolymer, polyethylene terephthalate (PET), a nylon, polyethylene, polypropylene, a thermoplastic elastomer, and copolymers and mixtures thereof, wherein the melting temperature of the inner contact layer and the outer layer differ by 30-120 °C.
A compact light source for coupling to a movable optic probe may include a light emitting diode (LED), to generate a probe signal at a first bandwidth, an optic coupler, disposed adjacent to the LED, and arranged to couple the probe signal into the movable probe, and a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth.
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01J 3/10 - Aménagements de sources lumineuses spécialement adaptées à la spectrométrie ou à la colorimétrie
G01J 3/42 - Spectrométrie d'absorptionSpectrométrie à double faisceauSpectrométrie par scintillementSpectrométrie par réflexion
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
Provided herein are affinity ligands that specifically interact with AAV8 capsid and/or AAV8 variant capsid, affinity agents comprising the affinity ligands, and methods of their use for binding, isolation, and/or purification of AAV8 and variants thereof.
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
50.
RECOVERY OF PERFUSION BLEED PRODUCT VIA ALTERNATING TANGENTIAL FLOW FILTRATION IN A SEDIMENTATION REACTOR
A bleed recovery system for use in a bioreactor system is disclosed. The bleed recovery system being configured to recover product of interest from the bleed material of the bioreactor system. In one embodiment, the bioreactor system includes a bleed recovery system for removing cell cultures from the bioreactor. The bleed recovery system includes a bleed pump coupled to the bioreactor so that cell bleed material can be removed from the bioreactor, a bleed vessel for receiving the cell bleed material, a cell retention device coupled to the bleed vessel, a harvest pump coupled to the cell retention device to deposit the product of interest into a second harvest tank, and a bleed pump connected to the bleed vessel to deposit the spent bleed material into a waste tank. Thus arranged, some or all of the product of interest can be separated from the spent medium.
A bleed recovery system for use in a bioreactor system is disclosed. The bleed recovery system being configured to recover product of interest from the bleed material of the bioreactor system. In one embodiment, the bioreactor system includes a bleed recovery system for removing cell cultures from the bioreactor. The bleed recovery system includes a bleed pump coupled to the bioreactor so that cell bleed material can be removed from the bioreactor, a bleed vessel for receiving the cell bleed material, a cell retention device coupled to the bleed vessel, a harvest pump coupled to the cell retention device to deposit the product of interest into a second harvest tank, and a bleed pump connected to the bleed vessel to deposit the spent bleed material into a waste tank. Thus arranged, some or all of the product of interest can be separated from the spent medium.
A system may include a light source, to generate a probe signal, comprising incident radiation; an optical probe, to direct the incident radiation through a fluid sample; a motor, to move the optical probe along a probe axis, to change a path length of the incident radiation through the fluid sample; and a detector, to receive the incident radiation as attenuated radiation after passing through the fluid sample. The system may include a control system, arranged to: initiate an absorbance measurement by directing movement of the optical probe along the probe axis; direct the light source to emit the incident radiation; and automatically adjust at least one measurement parameter of a set of measurement parameters for the sample measurement, based upon a slope parameter m, wherein m is derived from a rate of change in an intensity of the attenuated radiation with a change in the path length.
G01N 21/33 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière ultraviolette
54.
COMPACT HIGH RESOLUTION MONOCHROMATIC LIGHT SOURCE FOR FLUID SAMPLE CONCENTRATION MEASUREMENT
A compact light source for coupling to a movable optic probe may include a light emitting diode (LED), to generate a probe signal at a first bandwidth, an optic coupler, disposed adjacent to the LED, and arranged to couple the probe signal into the movable probe, and a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
G01N 21/85 - Analyse des fluides ou solides granulés en mouvement
G01N 21/33 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière ultraviolette
G01J 3/42 - Spectrométrie d'absorptionSpectrométrie à double faisceauSpectrométrie par scintillementSpectrométrie par réflexion
G01J 3/10 - Aménagements de sources lumineuses spécialement adaptées à la spectrométrie ou à la colorimétrie
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
56.
MULTI-MONOCHROMATIC LIGHT SOURCE SYSTEM FOR SLOPE SPECTROSCOPY
An apparatus may include a light emitting diode (LED) assembly, comprising a plurality of LEDs that output radiation at a plurality of different wavelengths, respectively. The LED assembly may be arranged to output a composite probe signal at a plurality of instances, wherein the composite probe signal comprises a plurality of probe signals, generated from the plurality of LEDs, respectively. The apparatus may also include a measurement module that includes an optic probe to direct the composite probe signal through a fluid sample, while moving between a plurality of probe positions at the plurality of instances. The measurement module may include a detector, disposed to detect, at the plurality of instances, a transmitted intensity of the composite probe signal at the plurality of different wavelengths after the composite probe signal passes through the fluid sample.
G01N 21/33 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière ultraviolette
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
G01N 21/85 - Analyse des fluides ou solides granulés en mouvement
58.
VARIABLE PATH LENGTH ABSORPTION SPECTROMETER HAVING AUTOMATED CONTINUOUS SLOPE MEASUREMENT
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
G01N 21/85 - Analyse des fluides ou solides granulés en mouvement
59.
MULTIMONOCHROMATIC LIGHT SOURCE SYSTEM FOR SLOPE SPECTROSCOPY
An apparatus may include a light emitting diode (LED) assembly, comprising a plurality of LEDs that output radiation at a plurality of different wavelengths, respectively. The LED assembly may be arranged to output a composite probe signal at a plurality of instances, wherein the composite probe signal comprises a plurality of probe signals, generated from the plurality of LEDs, respectively. The apparatus may also include a measurement module that includes an optic probe to direct the composite probe signal through a fluid sample, while moving between a plurality of probe positions at the plurality of instances. The measurement module may include a detector, disposed to detect, at the plurality of instances, a transmitted intensity of the composite probe signal at the plurality of different wavelengths after the composite probe signal passes through the fluid sample.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
G01N 21/85 - Analyse des fluides ou solides granulés en mouvement
60.
Encapsulated system for pressurized fluid processes
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale
F16K 27/02 - Structures des logementsMatériaux utilisés à cet effet des soupapes de levage
F16L 57/00 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes
F16L 57/02 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes contre la fissuration ou le flambement
If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
A61L 2/08 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des phénomènes physiques des radiations
62.
Fc binding proteins with cysteine in the C-terminal helical region
The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
A system for TFF filtration of a feedstock includes a feed vessel containing a solution comprising a target biomolecule, a filter membrane for filtering the solution, a feed pump for moving the solution from the feed vessel to the filter membrane, a buffer vessel coupled to the feed vessel by a buffer supply line, and a diafiltration pump disposed in the buffer supply line for delivering a buffer solution to the feed vessel. A variable path-length instrument is coupled to the feed line for determining, in real time, a concentration of the target biomolecule in the solution. A controller is coupled to the variable path-length instrument, the feed pump, and the diafiltration pump to control operation of the system based on the received information.
A system for TFF filtration of a feedstock includes a feed vessel containing a solution comprising a target biomolecule, a filter membrane for filtering the solution, a feed pump for moving the solution from the feed vessel to the filter membrane, a buffer vessel coupled to the feed vessel by a buffer supply line, and a diafiltration pump disposed in the buffer supply line for delivering a buffer solution to the feed vessel. A variable path-length instrument is coupled to the feed line for determining, in real time, a concentration of the target biomolecule in the solution. A controller is coupled to the variable path-length instrument, the feed pump, and the diafiltration pump to control operation of the system based on the received information.
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
G01N 15/06 - Recherche de la concentration des suspensions de particules
65.
FILTRATION UNITS AND ASSEMBLY, AND METHODS OF MAKING AND USING SAME
A self-contained filtration assembly with a self-contained filtration cassette therein. The cassette may be formed from one or more layers membranes and spacers (spacers, screens, or combinations thereof) all having the same general shape. The cassette may have a feed inlet path and a retentate outlet path extending through the layers of the cassette within the perimeter of the cassette, and a permeate outlet path between the cassette exterior and the interior of the cassette housing. The housing may have a generally circular or elliptical cross-section. A clamping assembly may hold together the components of the housing, and may be elongated in a direction along the flow path of the feedstream through the cassette. Various port-defining zones defined in the layers of the cassette may be sealed in a manner facilitating assembly of the cassette as well as isolation of the flow paths therein.
B01D 65/00 - Accessoires ou opérations auxiliaires, en général, pour les procédés ou les appareils de séparation utilisant des membranes semi-perméables
A self-contained filtration assembly with a self-contained filtration cassette therein. The cassette may be formed from one or more layers membranes and spacers (spacers, screens, or combinations thereof) all having the same general shape. The cassette may have a feed inlet path and a retentate outlet path extending through the layers of the cassette within the perimeter of the cassette, and a permeate outlet path between the cassette exterior and the interior of the cassette housing. The housing may have a generally circular or elliptical cross-section. A clamping assembly may hold together the components of the housing, and may be elongated in a direction along the flow path of the feedstream through the cassette. Various port-defining zones defined in the layers of the cassette may be sealed in a manner facilitating assembly of the cassette as well as isolation of the flow paths therein.
A method of improving volumetric productivity from a cell culture includes filtering a cell culture through an ultrafilter or a microfilter operating in tangential flow filtration mode or alternating flow filtration mode, and filtering the cell culture concurrently or intermittently through a tangential flow depth filtration system to remove cellular debris and/or to harvest cell product. A system for filtering biological materials includes a primary filtration system, and a secondary filtration system, where the secondary filtration system comprises a tangential flow depth filtration filter.
A method of improving volumetric productivity from a cell culture includes filtering a cell culture through an ultrafilter or a microfilter operating in tangential flow filtration mode or alternating flow filtration mode, and filtering the cell culture concurrently or intermittently through a tangential flow depth filtration system to remove cellular debris and/or to harvest cell product. A system for filtering biological materials includes a primary filtration system, and a secondary filtration system, where the secondary filtration system comprises a tangential flow depth filtration filter.
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
Vessel configured to facilitate concentration of a product in a solution. The vessel may include outlet ports to deliver the solution to a processing system (such as a filtration system), and inlet ports. The inlet ports may be at progressively lower levels for return of fluid thereto reduced in volume as a result of filtration thereof. A smaller volume sump may be provided below a main tank of the vessel to facilitate concentration of the solution. The sump may include ridges facilitating collection of the product of interest from the solution, and/or reinforcing the sump walls, and optionally forming a vortex breaker. A spray ball may be provided to spray materials, such as buffer solution, into the vessel. The spray ball may direct materials to facilitate retrieval of product from the vessel. One or more components of the vessel may be advantageously formed of disposable material for single use thereof.
Vessel configured to facilitate concentration of a product in a solution. The vessel may include outlet ports to deliver the solution to a processing system (such as a filtration system), and inlet ports. The inlet ports may be at progressively lower levels for return of fluid thereto reduced in volume as a result of filtration thereof. A smaller volume sump may be provided below a main tank of the vessel to facilitate concentration of the solution. The sump may include ridges facilitating collection of the product of interest from the solution, and/or reinforcing the sump walls, and optionally forming a vortex breaker. A spray ball may be provided to spray materials, such as buffer solution, into the vessel. The spray ball may direct materials to facilitate retrieval of product from the vessel. One or more components of the vessel may be advantageously formed of disposable material for single use thereof.
B01F 27/85 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec plusieurs agitateurs sur des arbres séparés
72.
TUBING COUPLER MOLDINGS AND SYSTEMS, AND ASSOCIATED METHODS
A tubing system with a coupler join positioned between and joining tubing segments. The coupler join is formed to reduce dead legs and hold up volumes within, and/or to reduce materials used to form, and/or to simplify the manufacturing process of the tubing system. The coupler join may be sized, shaped, configured, and/or dimensioned to join tubing segments, such as pre-formed tubing segments, in a streamlined configuration, such as to substantially correspond with the outer and inner diameters of the tubing segments. The tubing system and coupler join may be formed with a mold configured to form a coupler join securely coupling spaced apart ends of tubing segments to be joined together in a configuration which reduces dead legs and hold up volumes within the resulting tubing system.
B29C 45/14 - Moulage par injection, c.-à-d. en forçant un volume déterminé de matière à mouler par une buse d'injection dans un moule ferméAppareils à cet effet en incorporant des parties ou des couches préformées, p. ex. moulage par injection autour d'inserts ou sur des objets à recouvrir
B29C 65/70 - Assemblage d'éléments préformésAppareils à cet effet par moulage
B29C 65/00 - Assemblage d'éléments préformésAppareils à cet effet
B29K 83/00 - Utilisation de polymères contenant dans la chaîne principale uniquement du silicium avec ou sans soufre, azote, oxygène ou carbone comme matière de moulage
73.
Tubing coupler moldings and systems, and associated methods
A tubing system with a coupler join positioned between and joining tubing segments. The coupler join is formed to reduce dead legs and hold up volumes within, and/or to reduce materials used to form, and/or to simplify the manufacturing process of the tubing system. The coupler join may be sized, shaped, configured, and/or dimensioned to join tubing segments, such as pre-formed tubing segments, in a streamlined configuration, such as to substantially correspond with the outer and inner diameters of the tubing segments. The tubing system and coupler join may be formed with a mold configured to form a coupler join securely coupling spaced apart ends of tubing segments to be joined together in a configuration which reduces dead legs and hold up volumes within the resulting tubing system.
B29C 45/14 - Moulage par injection, c.-à-d. en forçant un volume déterminé de matière à mouler par une buse d'injection dans un moule ferméAppareils à cet effet en incorporant des parties ou des couches préformées, p. ex. moulage par injection autour d'inserts ou sur des objets à recouvrir
09 - Appareils et instruments scientifiques et électriques
Produits et services
Laboratory consumables kits for use with cell culture media analyzers comprised primarily of microfluidic chips for cell culture analysis, laboratory microplates, and covers for laboratory microplates, and also consisting of background electrolytes for cell culture analysis, and diluent for cell culture analysis
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Laboratory consumables kits for use with cell culture media analyzers comprised primarily of chemicals in the nature of standard solutions used in analytical chemistry
A filter screening tool, system, and method allowing high throughput screening of filters (such as hollow fiber filters), such as for use in bioprocessing workflows. Feed material may be fed through different filter modules to assess, measure, analyze, determine, evaluate, teste, compare, screen, etc., properties or characteristics, etc., of filters within the filter modules. Each filter module may house a different type (e.g., material or configuration) of filter so that different filters may be compared and screened, such as for a selected use.
The present disclosure is related to bioprocessing devices, systems, and methods, and particularly to sanitary connections for bioprocessing. The devices, systems, and methods described herein can relate to the sanitary connection of flow fittings, such as reducers, with considerations for ease of assembly and sealing. In some embodiments, the system may include a flow reducer, a ferrule and a gasket. The flow reducer may include a gasket channel, an overmold channel within the gasket channel, and one or more cavities within the overmold channel. The gasket may be overmolded within the gasket channel and extending within the one or more cavities.
F16L 25/14 - Raccords pour tuyaux de diamètre ou de section transversale différents
F16L 21/03 - Raccords avec manchon ou douille avec segments d'étanchéité élastiques entre le tuyau et le manchon ou entre le tuyau et la douille, p. ex. avec des segments roulants ou autres segments profilés préfabriqués placés dans la partie femelle avant la fixation
09 - Appareils et instruments scientifiques et électriques
Produits et services
Detection and identification instruments using light sources and light detectors for detecting, identifying, and quantifying chemical and biological substances not for medical purposes; Scientific apparatus, namely, spectrometers and parts, fittings, probes, fiberoptic cables thereof.
80.
ALTERNATING TANGENTIAL FLOW BIOREACTOR WITH HOLLOW FIBER SYSTEM AND METHOD OF USE
Embodiments of the present disclosure relate generally to systems and methods for perfusion cell culture involving alternating fluid flows between first and second flexible vessels. For example, a hollow fiber filter module may be attached to first and second culture vessels which each include inner and outer vessels. A pressure source may cause a pressure differential between the outer vessels, which may cause a responsive fluid flow between the inner vessels across a hollow fiber filtration unit.
A pressure gauge and/or relief valve (100) which may be used in a single-use fluid system, such as a sterilizable fluid system. The pressure gauge and/or pressure relief valve may be formed of a disposable and/or sterilizable material, such as a non-metallic (e.g., polymeric) material. The material of the pressure gauge and/or pressure relief valve may be compatible with the material of a fluid-containing structure of the fluid system, such as to be bonded (e.g., welded) therewith. The fluid-containing structure may be retrofitted to integrate the pressure gauge and/or pressure relief valve therewith. The pressure gauge and/or pressure relief valve may have a simple configuration, such as a housing (120) with a movable element (130) therein. The movable element moves with respect to the housing to indicate pressure and/or to relieve pressure from within the housing and/or fluid-containing structure.
F16K 17/04 - Soupapes ou clapets de sûretéSoupapes ou clapets d'équilibrage ouvrant sur excès de pression d'un côtéSoupapes ou clapets de sûretéSoupapes ou clapets d'équilibrage fermant sur insuffisance de pression d'un côté actionnés par ressort
F16K 37/00 - Moyens particuliers portés par ou sur les soupapes ou autres dispositifs d'obturation pour repérer ou enregistrer leur fonctionnement ou pour permettre de donner l'alarme
A61M 5/168 - Moyens pour commander l'écoulement des agents vers le corps ou pour doser les agents à introduire dans le corps, p. ex. compteurs de goutte-à-goutte
G01L 19/06 - Moyens pour empêcher la surcharge ou l'influence délétère du milieu à mesurer sur le dispositif de mesure ou vice versa
82.
Pressure gauge relief valve for sterilizable fluid system
A pressure gauge and/or relief valve which may be used in a single-use fluid system, such as a sterilizable fluid system. The pressure gauge and/or pressure relief valve may be configured to measure and to relieve fluid within a fluid-containing structure, the pressure gauge relief valve including a housing having a first end, a second end, and an inner surface defining a passage between the first end and the second end, a movable element movable within the passage, wherein the housing first end is configured to be coupled with a fluid-containing structure, the housing second end is spaced from the housing first end; and the movable element is movable with respect to the housing to indicate pressure therein and to allow fluid to pass between the movable element and the inner surface of the passage at a selected pressure.
G01L 19/00 - Détails ou accessoires des appareils pour la mesure de la pression permanente ou quasi permanente d'un milieu fluent dans la mesure où ces détails ou accessoires ne sont pas particuliers à des types particuliers de manomètres
G01L 19/06 - Moyens pour empêcher la surcharge ou l'influence délétère du milieu à mesurer sur le dispositif de mesure ou vice versa
F16K 17/04 - Soupapes ou clapets de sûretéSoupapes ou clapets d'équilibrage ouvrant sur excès de pression d'un côtéSoupapes ou clapets de sûretéSoupapes ou clapets d'équilibrage fermant sur insuffisance de pression d'un côté actionnés par ressort
A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
B01D 29/11 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants en forme de sac, de cage, de tuyau, de tube, de manchon ou analogue
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
An encapsulated valve system includes a first housing portion having a first facing surface, the first facing surface comprising a plurality of branch pathways formed as a recess within the first facing surface. The valve system further includes a second housing portion having a second facing surface, the second facing surface comprising a plurality of branch pathways formed as a recess within the second facing surface. A disposable conduit is configured to be interposed between the first and second housing portions and disposed within the recess of the first facing surface and the recess of the second facing surface. The disposable conduit is thus sandwiched between the first and second facing surfaces. A plurality of pinch valve actuators are mounted on one or both of the first housing portion and the second housing portion, the plurality of pinch valve actuators configured to pinch the disposable conduit at selective branch pathways.
F16K 7/04 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale
F16K 7/07 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par pression d'un fluide
F16K 27/00 - Structures des logementsMatériaux utilisés à cet effet
F16K 7/00 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture
F16K 31/06 - Moyens de fonctionnementDispositifs de retour à la position de repos électriquesMoyens de fonctionnementDispositifs de retour à la position de repos magnétiques utilisant un aimant
F16K 7/06 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale par tige filetée, came ou autre moyen mécanique
F16K 11/02 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout
F16K 11/10 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont plusieurs éléments de fermeture ne se déplacent pas comme un tout
F16K 3/30 - Robinets-vannes ou tiroirs, c.-à-d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture Détails
86.
Encapsulated system for pressurized fluid processes
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale
F16L 57/00 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes
F16L 57/02 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes contre la fissuration ou le flambement
F16K 27/02 - Structures des logementsMatériaux utilisés à cet effet des soupapes de levage
87.
Plunger pumping arrangement for a hollow fiber filter
A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion of the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.
B01D 29/11 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants en forme de sac, de cage, de tuyau, de tube, de manchon ou analogue
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
F04B 43/00 - "Machines", pompes ou installations de pompage ayant des organes de travail flexibles
F04B 43/06 - Pompes ayant un entraînement par fluide
09 - Appareils et instruments scientifiques et électriques
Produits et services
Scientific instruments, namely, electronic monitors comprised of software and hardware for measuring and analyzing in real time chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants.
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Scientific instruments, namely, electronic analyzers comprised of computer software and computer hardware for measuring and analyzing in real time chemical and biological substances for the presence, absence, and quantity of target chemicals, biologics, and contaminants.
C12N 15/64 - Méthodes générales pour la préparation du vecteur, pour son introduction dans la cellule ou pour la sélection de l'hôte contenant le vecteur
B01D 61/00 - Procédés de séparation utilisant des membranes semi-perméables, p. ex. dialyse, osmose ou ultrafiltrationAppareils, accessoires ou opérations auxiliaires, spécialement adaptés à cet effet
B01D 29/60 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants combinés dans une même structure à des dispositifs de commande de la filtration
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
B01D 29/11 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants en forme de sac, de cage, de tuyau, de tube, de manchon ou analogue
B01D 29/66 - Rinçage par chasse, p. ex. rafale ascendante d'air à contre-courant
B01D 65/02 - Nettoyage ou stérilisation de membranes
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
93.
Filtration system and method for synchronizing permeate and retentate flow
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
B01D 29/11 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants en forme de sac, de cage, de tuyau, de tube, de manchon ou analogue
B01D 29/66 - Rinçage par chasse, p. ex. rafale ascendante d'air à contre-courant
B01D 29/90 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants comportant des dispositifs d'alimentation ou d'évacuation d'alimentation
B01D 65/02 - Nettoyage ou stérilisation de membranes
The present disclosure relates to hollow fiber tangential flow filter units, including hollow fiber tangential flow depth filter units, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.
B01D 29/00 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants
B01D 29/11 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants en forme de sac, de cage, de tuyau, de tube, de manchon ou analogue
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.
F04B 49/20 - Commande des "machines", pompes ou installations de pompage ou mesures de sécurité les concernant non prévues dans les groupes ou présentant un intérêt autre que celui visé par ces groupes en modifiant la vitesse d'entraînement
F04B 43/00 - "Machines", pompes ou installations de pompage ayant des organes de travail flexibles
F04B 23/02 - Installations ou systèmes de pompage comportant des réservoirs
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
The present disclosure relates to hollow fiber tangential flow filter units, including hollow fiber tangential flow depth filter units, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
B01D 63/04 - Modules à fibres creuses comprenant plusieurs ensembles à fibres creuses
Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
99.
Encapsulated system for pressurized fluid processes
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Dispositifs d'obturation à diaphragme, p. ex. dont un élément est déformé, sans être déplacé entièrement, pour fermer l'ouverture à diaphragme tubulaire dont l'étranglement est assuré par une force extérieure radiale
F16L 57/02 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes contre la fissuration ou le flambement
F16K 27/02 - Structures des logementsMatériaux utilisés à cet effet des soupapes de levage
F16L 57/00 - Protection des tuyaux ou d'objets de forme similaire contre les dommages ou les usures internes ou externes
100.
Methods and systems for removing pressure and air from chromatography columns
Methods and systems for removing gases and/or pressure formed during the sterilization, e.g., the gamma irradiation, of prepacked chromatography systems (column plus attached tube and valve set) are described. The methods include purging the gas and/or pressure through specially designed tube and valve sets without breaching the sterility of the prepacked sterile chromatography system. The systems include a sterile or aseptic pre-packed chromatography column including a column having an inlet and an outlet, a tubing and valve set attached to the inlet and the outlet, and a pump configured to pump sterile or aseptic liquid from the fluid source along the tubing and valve set, into the column tube inlet and out of the column tube outlet along a first flow path, thereby removing any entrapped gas and/or pressure from the chamber.
B01D 15/10 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement
A61L 2/08 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des phénomènes physiques des radiations
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01J 39/04 - Procédés utilisant des échangeurs organiques
B01J 39/19 - Composés macromoléculaires obtenus autrement que par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 39/26 - Échangeurs de cations pour procédés chromatographiques
G01N 30/50 - Conditionnement de l'absorbant ou de l'adsorbant ou de la phase liquide stationnaire
