Provided herein are systems, compositions, and methods for single cell analysis. In particular, provided herein are systems, compositions, and methods for scalable, high-throughput isolation and sequencing of nucleic acids from rare and/or fragile single cells.
Provided herein are compositions and methods for amplifying long nucleic acid molecules. In particular, provided herein are compositions and methods for amplifying long nucleic acid molecules that are mixed in complex samples, for example, complex biological samples.
Provided herein are compositions and methods for amplifying long nucleic acid molecules. In particular, provided herein are compositions and methods for amplifying long nucleic acid molecules that are mixed in complex samples, for example, complex biological samples.
Provided herein systems, compositions, and methods for nucleic acid quantification that combines the sensitivity and accuracy of digital PCR with the capability for high-throughput multiplexing. The methods integrate digital amplification in compartments with capillary electrophoresis or other methodologies as a readout mechanism.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C40B 70/00 - Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or barcodes
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
6.
CORE-SHELL GEL PARTICLES FOR SEPARATING AND ANALYZING RNA AND DNA FROM A CELL
Provided herein are compositions, systems, kits, and methods for separating and analyzing RNA/cDNA from genomic DNA molecules using core-shell gel particles (e.g., such that separate barcoded sequencing libraries can be generated from each). In certain embodiments, the core-shell gel particles comprise an outer gel shell, and an interior region surrounded by said shell that comprises: A) an interior solution that is at least partially aqueous, B) optionally an inner gel core; C) at least one cell, and D) a plurality of capture beads comprising capture probes configured to hybridize to RNA from the cell, and optionally configured to hybridize to genomic DNA and/or bind target proteins.
Provided herein are devices, assemblies, systems, kits, and methods for generating an emulsion with a microfluidic chamber composed of impediments (e.g., posts) regularly or irregularly spaced apart to create pores (e.g., 10-1000 microns in diameter). In certain embodiments, a non-emulsion mixture composed of sample fluid (e.g., containing cells and/or beads) and an immiscible carrier fluid are flown through the microfluidic chamber once or repeatedly such that an emulsion is generated comprising sample microdroplets (e.g., about the same size as the pores, or of irregular shape). In some embodiments, the microfluidic chamber is housed in a pipette tip and a micropipette is used to push or draw the non-emulsion mixture through the microfluidic chamber.
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
11.
SYSTEMS, COMPOSITIONS, AND METHODS FOR SINGLE CELL ANALYSIS
Provided herein are systems, compositions, and methods for single cell analysis. In particular, provided herein are systems, compositions, and methods for scalable, high-throughput isolation and sequencing of nucleic acids from rare and/or fragile single cells.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
patents
