A method for manufacturing a multipole assembly of a mass spectrometer, the method comprising: machining a body to form an opening extending along a predetermined axis through the body, wherein the opening has a profile that defines a pair of opposed seats, each seat being configured to guide a rod of the multipole assembly, cutting the body into a plurality of pieces to form a plurality of identical rod supports, seating a first pair of rods in the seats of a first pair of the rod supports, and seating a second pair of rods in the seats of a second pair of the rod supports, wherein the second pair of rod supports are rotated relative to the first pair of rod supports.
A gas delivery system for a gas analysis system, comprising a pressurised gas source, a pressure regulator for regulating the pressure of the gas flow from the gas source, an input line in communication with a reservoir for supplying a regulated gas flow from the pressure regulator to the reservoir at a pressure of at least 101 kPa, wherein the reservoir contains a material to be entrained in the gas flow, and an outlet is provided for the entrained gas flowing from the reservoir.
A mass or mobility spectrometer comprising: a first vacuum chamber; and a collision cell arranged in the first vacuum chamber, wherein the collision cell comprises: an ion entrance at an upstream end thereof; an ion exit at a downstream end thereof; a main body portion between the ion entrance and ion exit; and an elongated tube between the main body portion and the ion exit. The collision cell comprises one or more ion guide extending through the main body portion, and/or through the elongated tube, for radially confining ions within the one or more ion guide. The spectrometer is configured to maintain the main body portion of the collision cell at a higher pressure than the first vacuum chamber; and the elongated tube is configured to limit the gas conductance out of the downstream end of the collision cell.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A method of mass spectrometry comprising: a) separating a sample in a separator device and then mass analysing the sample so as to obtain a list of sample peaks in which a mass to charge ratio for each peak is associated with a retention time in the separator device; b) providing a list of library peaks in which a mass to charge ratio for each peak is associated with a retention time in a separator device; c) dividing the list of sample peaks, according to retention time, into multiple sample peak sub-lists, and dividing the list of library peaks, according to retention time, into multiple library peak sub-lists; d) peak- matching at least a first peak in a first of the sample peak sub-lists with at least a first respective peak in a first of the library peak sub-lists so as to obtain at least a first pair of matched peaks; e) determining an error in the mass to charge ratio of the first peak in the first sample peak sub-list based on the mass to charge ratios of the peaks in the first pair of matched peaks; and f) adjusting the mass to charge ratio of at least the first peak in the first sample peak sub-list using said error in mass to charge ratio so as to provide an adjusted first sample peak sub-list.
Improved multi-pass time-of-flight mass spectrometers MPTOF, either multi-reflecting (MR) or multi-turn (MT) TOF are proposed with elongated pulsed converters—either orthogonal accelerator or radially ejecting ion trap. The converter 35 is displaced from the MPTOF s-surface of isochronous ion motion in the orthogonal Y-direction. Long ion packets 38 are pulsed deflected in the transverse Y-direction and brought onto said isochronous trajectory s-surface, this way bypassing said converter. Ion packets are isochronously focused in the drift Z-direction within or immediately after the accelerator, either by isochronous trans-axial lens/wedge 68 or Fresnel lens. The accelerator is improved by the ion beam confinement within an RF quadrupolar field or within spatially alternated DC quadrupolar field. The accelerator improves the duty cycle and/or space charge capacity of MPTOF by an order of magnitude.
A method of separating ions comprising: rotating a housing about an axis such that gas therein is rotated around the axis; and then transmitting ions into the gas such that the ions are driven around the axis by the gas and thus experience a centrifugal force that separates the ions.
A method of mass spectrometry is disclosed comprising: a step 10 of analysing a reference compound in a first mass spectrometer and outputting mass spectral data in response thereto; a step 20 of analysing the reference compound in a second, different mass spectrometer and outputting mass spectral data in response thereto; and a step 30 of automatically adjusting an operational parameter, duty cycle (e.g. duty cycle of data acquisition), or acquired spectral data of at least one mass spectrometer such that, for a given consumption of reference compound by the spectrometer, the statistical precision of quantification and/or mass measurement by the mass spectrometer is substantially the same as that of another mass spectrometer (for the same given consumption of the analyte).
A mass and/or mobility spectrometer comprising: an ion source enclosure for housing an ion source: a vacuum chamber; a pumping block between the ion source enclosure and vacuum chamber; and a shield for protecting a surface of the ion source enclosure; wherein the shield has a first portion configured to mount to a upstream side of the pumping block so as to secure the shield at a location in which a second portion of the shield covers an internal surface of the ion source enclosure.
A method of determining the enantiomeric purity of an analyte, comprising: ionising the analyte to form dimer ions; separating ions of different dimers of the analyte by ion mobility; detecting a first ion mobility peak corresponding to ions of one or more first dimers and detecting a second ion mobility peak corresponding to ions of one or more second dimers; and determining the enantiomeric purity of said analyte from the ratio of the peak area of the first ion mobility peak to the peak area of the second ion mobility peak.
A method of mass spectrometry comprising: a) providing a mass spectrometer having a time of flight (TOP) mass analyser: b) performing a survey scan comprising separating a packet of precursor ion species and mass analysing ions so as to obtain first mass spectral data: c) determining a time window over which one of the precursor ion species. or fragment or product ions derived therefrom, were mass analysed: d) separating another packet of precursor ion species and mass analysing ions so as to obtain second mass spectral data. wherein the pusher of the TOP mass analyser is pulsed according to a plurality of consecutive pulse sequences during a plurality of respective pulse sequence time periods. wherein each pulse sequence consists of consecutive pushes that are arranged such that the duration between any pair of pushes in the pulse sequence is different to the duration between any other pair of pushes within the pulse sequence: f) selecting mass spectral data, from the second mass spectral data, that was obtained during a time period corresponding to said time window of the survey scan, so as to obtain selected data; g) repeating steps d) to f) at least one further time such that multiple sets of said selected data are obtained; combining said multiple sets of selected data and decoding the combined data to obtain mass spectral data representative of the mass to charge ratios of the ions detected by the TOP mass analyser.
A method of mass spectrometry comprising: a) accumulating precursor ions in a first ion accumulator; b) performing a separation cycle comprising pulsing a packet of the precursor ions out of the first ion accumulator and into an ion separator, and separating the precursor ions such that precursor ions having different values of a first physicochemical property elute from the ion separator at different times; c) mass filtering the precursor ions that elute from the ion separator so as to transmit a selected precursor ion species; d) fragmenting or reacting the selected precursor ion species so as to generate a set of fragment or product ion species therefrom; e) accumulating the set of fragment or product ion species in a second ion accumulator; f) releasing the set of fragment or product ion species from the second ion accumulator into a TOP mass analyser, wherein operation of the TOP mass analyser is synchronised with the release of ions from the second ion accumulator such that multiple different species of the set of fragment or product ion species are simultaneously pulsed into a time of flight region of the TOP mass analyser by a pusher electrode; and g) repeating steps c) to f) at least once during said separation cycle, wherein said selected precursor ion species is different each time steps c) to f) are performed.
A method of mass spectrometry comprising: performing a first MSMS analysis in which ions having a first mass to charge ratio detected in a survey mode are fragmented or reacted and then mass analysed so as to obtain first MSMS mass spectral data; adding a mass to charge ratio detected in the first MSMS mass spectral data to an exclusion list; performing a second MSMS analysis in which ions having a second, different mass to charge ratio detected in the survey mode are fragmented or reacted, wherein the method comprises checking that the second mass to charge is not present on the exclusion list prior to performing the second MSMS analysis.
A method of analysing ions is disclosed comprising: (i) subjecting ions of an analyte molecule to different activation levels at different times so as to cause the ions to have different mobilities at said different times, wherein the activation level is varied in a plurality of cycles, and wherein the activation level is varied between said different levels during each of the cycles. The method uses an ion mobility separator or scanned ion mobility filter to determine the mobilities of the ions for said different activation levels; and correlates the determined mobilities with their respective activation levels so as to thereby obtain a fingerprint for the analyte molecule.
A Time of Flight mass analyser comprising: an ion detector; a pusher (3) configured to perform a sequence of pushes for pushing packets of ions towards the ion detector; and an ion attenuator (5) configured to alternate between a high transmission mode in which it allows ions to reach the ion detector and a low transmission mode in which it prevents ions from reaching the ion detector. The mass analyser is configured to synchronise the operation of the ion attenuator (5) in the high and low transmission modes with the timings of the pushes such that a first plurality of the pushes result in ions being received at the ion detector and a second different plurality of the pushes do not result in ions being received at the detector.
A method of separating ions according to their ion mobility using an ion mobility separation device including a first section and a second section is disclosed, comprising: urging ions through the first section against a first opposing electric field using a first driving force provided by a first set of time varying voltage(s) or voltage waveform(s); progressively reducing the magnitude of the first opposing electric field and/or progressively increasing the magnitude of the first driving force; and driving ions through the second section against a second opposing electric field using a second drive force provided by a second set of time-varying voltage(s) or voltage waveform(s); wherein the magnitude of the second opposing electric field is progressively reduced and/or the magnitude of the second driving force is progressively increased in tandem with reducing the magnitude of the first opposing electric field and/or increasing the magnitude of the first driving force.
Disclosed are methods of mass spectrometry that use charge reduction techniques to reduce the average charge of the ions to increase the mass to charge ratio spacings between different charge states for the ion species prior to mass analysis. In embodiments there is disclosed a method for determining a collision cross section for an ion species, wherein an ion mobility is determined for an ion species in a first state without charge reduction and the mass to charge ratios for the ion species are then measured in a second, charge-reduced state. The average charge determined for the ion species in the first state is then used together with the average mass determined from the ion species in the second state and the ion mobility determined for the ion species in the first state to determine a collision cross section for the ion species in the first state.
A nebuliser outlet comprises an inlet end and an outlet end, a first channel and one or more second channels arranged between the inlet end and the outlet end. The first channel is configured to receive a capillary, and the one or more second channels are configured to pass gas to the outlet end. The nebuliser outlet is a single integrated component.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
B33Y 80/00 - Produits obtenus par fabrication additive
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
An ion-electron reaction device comprises a first tubular magnet, a second tubular magnet arranged coaxially with the first tubular magnet, and a shim located between the first and second tubular magnets. The shim is arranged and configured such that the first and second tubular magnets provide a substantially homogenous magnetic field along a central axis of the device.
A mass and/or mobility spectrometer comprising: an ion attenuation device comprising an ion guide 6 and an ion attenuator 10 arranged within the ion guide, wherein the ion attenuator is configured to repeatedly switch between a high attenuation mode in which it attenuates ions at a first rate and a low attenuation mode in which it attenuates ions at a lower rate. The spectrometer is also configured to maintain the ion attenuation device at a pressure such that different groups of ions that are transmitted by the ion attenuator in different respective low attenuation modes merge with each other downstream of the ion attenuator.
A method of detecting one or more PFAS in a sample of animal tissue, comprising: spraying electrically charged solvent droplets onto the sample of animal tissue so as to generate analyte ions from analyte in the animal tissue; passing the analyte ions into a mass spectrometer; and mass analysing the analyte ions so as to determine if the one or more PFAS is in the animal tissue.
G01N 33/84 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des composés inorganiques ou le pH
Disclosed herein are various methods and apparatus for performing charge detection mass spectrometry (CDMS). In particular, techniques are disclosed for monitoring a detector signal from a CDMS device to determine how many ions are present in the ion trap (10) of the CDMS device. For example, if no ions are present the measurement can then be terminated early. Similarly, if more than one ion is present, the measurement can be terminated early, or ions can be removed from the trap (10) until only a single ion remains. Techniques are also provided for increasing the probability of there being a single ion in the trap (10). A technique for attenuating an ion beam is also provided.
A method of mass spectrometry comprising: mass analysing ions with a mass analyser so as to obtain first mass spectral data: summing the first mass spectral data obtained during a first integration period: obtaining a transmission profile indicative of how the transmission level of first ions to said mass analyser would vary with time during the first integration period; and determining an ion arrival rate of said first ions at the mass analyser during the first integration period based on said transmission profile.
There is provided a method of operating an electrostatic ion trap, the electrostatic ion trap including a plurality of electrodes, the method including setting the voltage of the plurality of electrodes to a first voltage map, wherein the voltage map is configured such that: at a first ion kinetic energy per unit charge (KE) range, more than a first percentage of ions are stable, at a second ion KE range, less than a second percentage of ions are stable, and at a third ion KE range, more than the first percentage of ions are stable, wherein the first percentage is at least 4 times greater than the second percentage, and wherein the second ion KE range is between the first ion KE range and the third ion KE range.
A method of correcting mass spectral data comprises making calibration measurements at one or more calibration times using calibrants which have known mass to charge ratio (m/z) values or previously mass measured mass to charge ratio (m/z) values, making a list of intrinsic components which are present during more than one acquisition periods, wherein the components have mass to charge ratio (m/z) values that were not present or observed during or close to the one or more calibration times, and utilising the list to calculate a mass or mass to charge ratio (m/z) correction factor for one or more acquisition periods which are not close or adjacent in time to an acquisition period containing a directly calibrated mass to charge ratio (m/z) value.
A temperature monitoring system (16) for use with a thermocouple (21) and a heater controller (24) to control a heater (1). The system (16) comprises a differential amplifier (17) which comprises a first input terminal (18) which is configured to connect to a first terminal (20) of the thermocouple (21) and a second input terminal (19) which is configured to connect to a second terminal (22) of the thermocouple (21). The differential amplifier (17) comprises an output terminal (23) which is configured to provide a temperature sense signal to the heater controller to control the heater (1). The system (16) further comprises a protection circuit (24) which is connected to the first input terminal (18) and the second input terminal (19). The protection circuit (24) is configured to operate in a first mode in the event that a leakage current flows into the first input terminal (18) or the second input terminal (19); and a second mode in the event that a leakage current flows out from the first input terminal (18) or the second input terminal (19), such that the protection circuit (24) controls the differential amplifier (17) to output a temperature sense signal which controls the heater (1) to operate at a temperature at or below a predetermined temperature to reduce the risk of damage being caused by the heater (1).
H05B 1/02 - Dispositions de commutation automatique spécialement adaptées aux appareils de chauffage
G01K 7/02 - Mesure de la température basée sur l'utilisation d'éléments électriques ou magnétiques directement sensibles à la chaleur utilisant des éléments thermo-électriques, p. ex. des thermocouples
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A nebuliser outlet (114) comprises an outer wall (304) and a liquid capillary support conduit (314) arranged radially inward of the outer wall (304) for radially confining a liquid capillary (106) therein. A radial support structure (318) radially connects the liquid capillary support conduit (314) to the outer wall (304) in a manner such that one or more gas channels (320) are provided radially between the outer wall (304) and the liquid capillary support conduit (314). A first annular channel (322) surrounds the liquid capillary support conduit (314) at a first position that is axially upstream of the radial support structure (318). A second annular channel (324) surrounds the liquid capillary support conduit (314) at a second position that is axially downstream of the radial support structure (318). The one or more gas channels (320) connect the first and second annular channels (322, 324).
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
An electrospray apparatus (130) comprises a capillary (102) having an outlet orifice (108) for electrospraying a sample therefrom and a conduit (112) extending into the capillary (102) in a manner such that it is able to deliver said sample into the capillary (102). The apparatus (130) may be configured to supply a pressurising gas into the capillary (102) so as to force said sample within the capillary (102) towards the outlet orifice (108). The apparatus may comprise a sheath member (203) surrounding at least a portion of the capillary (102), wherein the sheath member (102) has one or more apertures (213) located therein for allowing the capillary (102) to be viewed, and the apparatus may comprise a connector element (205) for connecting the sheath member (203) to, in order to position the capillary within the electrospray apparatus (130).
A method of mass spectrometry is disclosed comprising: a) providing temporally separated precursor ions; b) mass analyzing separated precursor ions, and/or product ions derived therefrom, during a plurality of sequential acquisition periods, wherein the value of an operational parameter of the spectrometer is varied during the different acquisition periods; c) storing the spectral data obtained in each acquisition period along with its respective value of the operational parameter; d) interrogating the stored spectral data and determining which of the spectral data for a precursor ion or product ions meets a predetermined criterion, and determining the value of the operational parameter that provides this mass spectral data as a target operational parameter value; and e) mass analyzing again the precursor or product ions whilst the operational parameter is set to the target operational parameter value.
An ion detector for a mass and/or ion mobility spectrometer is disclosed. The ion detector comprises a dynode arranged and configured such that primary ions to be detected by the ion detector impact upon the dynode and generate first electrons and secondary positive ions, an electron detector arranged and configured to attract and detect said first electrons, and an apertured electrode. The apertured electrode comprises a plurality of apertures and is arranged and configured such that at least some of said secondary positive ions pass through the apertures of the electrode.
A lens assembly for an outer source of a mass spectrometer, the assembly comprising: a housing; a plurality of lens elements, each lens element comprising a radially extending electrically conductive protrusion, the lens elements being linearly arranged in the housing such that the protrusions are aligned with one another, and an interface connector having a plurality of sockets to receive the protrusions therein and create an electrical connection between the protrusions and sockets. A housing for a resistance temperature detector is also disclosed.
An ion source assembly comprising: a target plate for holding a sample to be analysed; a laser for ionising the sample on the target plate so as to form analyte ions; one or more optical elements; an ion guide for guiding the analyte ions; a first gas port arranged to supply a first gas stream so as to urge material generated at the target plate away from the one or more optical element; and a second, different gas port arranged to supply a second gas stream that urges ions from the target plate, towards and into the ion guide.
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/14 - Sources d'ionsCanons à ions utilisant un bombardement de particules, p. ex. chambres d'ionisation
A method of mass filtering ions is disclosed comprising: providing a first, AC-only, mass filter 2; providing a second mass filter 4 downstream of the first mass filter; applying a first AC voltage 8 to electrodes of the first mass filter so as to radially confine ions between the electrodes, and applying a second AC voltage 10 between electrodes of the first mass filter 2 so as to radially excite some of said ions such that these ions are not transmitted; and using the second mass filter 4 to mass filter ions; wherein at any given time the second mass filter 4 only transmits ions having a first range of mass to charge ratios and filters out all other ions; and wherein the step of applying the at least one second AC voltage 10 to electrodes of the first mass filter 2 radially excites ions such that at least some ions having mass to charge ratios above said first range are not transmitted into the second mass filter.
A method is disclosed comprising: trapping ions in an ion trap (40); applying a first force on the ions within the ion trap in a first direction, said force having a magnitude that is dependent upon the value of a physicochemical property of the ions; applying a second force on these ions in the opposite direction so that the ions separate according to the physicochemical property value as a result of the first and second forces; and then pulsing or driving ions out of one or more regions of the ion trap.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
G01N 27/62 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'ionisation des gaz, p. ex. des aérosolsRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant les décharges électriques, p. ex. l'émission cathodique
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
H01J 49/42 - Spectromètres à stabilité de trajectoire, p. ex. monopôles, quadripôles, multipôles, farvitrons
A method of mass and/or mobility spectrometry comprising: providing an ion separation device comprising a plurality of electrodes; providing a first force that urges ions in a first direction along an axis of the ion separation device, whilst also providing a second force that urges ions in a second opposite direction such that ions of different mobility or mass to charge ratio reach different equilibrium positions at different locations along the axis; and varying the first and/or second force during a single elution cycle of the ion separation device so as to sequentially release ions from said ion separation device in increasing order of ion mobility or mass to charge ratio, or in decreasing order of ion mobility or mass to charge ratio; wherein said varying step is performed such that ions in a first range of mobilities or mass to charge ratios elute from the ion separation device at a first rate, and ions in a second separate range of mobilities or mass to charge ratios elute from the ion separation device at a second different rate during said elution cycle.
A connector comprising a connector body configured for connection to an identical connector body, the connector body having a male part and a female part, which have profiles that conform, at least partially, to one another.
H01R 24/84 - Dispositifs de couplage hermaphrodite
H01R 13/41 - Fixation d'une manière non démontable, p. ex. par moulage, rivetage par engagement à frottement dans une rondelle isolante, un panneau ou une base
H01R 13/66 - Association structurelle avec des composants électriques incorporés
H01R 24/20 - Pièces de couplage portant des douilles, des pinces ou des contacts analogues, assujetties uniquement à un fil ou un câble
H01R 24/28 - Pièces de couplage portant des broches, des lames ou des contacts analogues, assujetties uniquement à un fil ou un câble
A nebuliser outlet assembly comprises an inlet end and an outlet end, and a first channel (24) arranged between the inlet end and the outlet end, wherein the first channel is configured to receive a capillary. The nebuliser outlet assembly comprises a first part (40) and one or more second parts (50), wherein the one or more second parts are removably attachable to the first part. The nebuliser outlet assembly is configured such that the first channel is formed from the first part and the one or more second parts when the one or more second parts are attached to the first part. The first part comprises an open-sided channel that corresponds to the first channel for at least some of its length. The nebuliser outlet assembly is configured such that when the one or more second parts are removed from the first part, said open-sided channel is exposed.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
37.
MASS SPECTROMETER HAVING PARALLEL PRECURSOR ION ISOLATION
A method of mass spectrometry comprising: providing a mass spectrometer comprising a mass filter (2), an ion mobility separator (IMS) device (3), a fragmentation or reaction device (4), and a mass analyser (5); applying voltages to the mass filter such that it simultaneously has multiple mass transmission windows that simultaneously transmit multiple respective precursor ion species to the IMS device whilst filtering out other precursor ion species; separating, in the IMS device, the precursor ion species that are simultaneously transmitted by the mass filter so as to provide different ones of the precursor ion species to the fragmentation or reaction device at different times; sequentially fragmenting or reacting the different precursor ion species in the fragmentation or reaction device so as to form fragment or product ions; detecting the fragment or product ions in the mass analyser; and associating detected fragment or product ions with their respective precursor ion species based on the times of detection of the fragment or product ions in the mass analyser.
A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; and mass analysing ions.
A method of introducing and ejecting ions from an ion entry/exit device 4 is disclosed. The ion entry/exit device 4 has at least two arrays of electrodes 20,22. The device is operated in a first mode wherein DC potentials are successively applied to successive electrodes of at least one of the electrode arrays 20,22 in a first direction such that a potential barrier moves along the at least one array in the first direction and drives ions into and/or out of the device in the first direction. The device is also operated in a second mode, wherein DC potentials are successively applied to successive electrodes of at least one of the electrode arrays 20,22 in a second, different direction such that a potential barrier moves along the array in the second direction and drives ions into and/or out of the device in the second direction. The device provides a single, relatively simple device for manipulating ions in multiple directions. For example, the device may be used to load ions into or eject ions from an ion mobility separator in a first direction, and may then be used to cause ions to move through the ion mobility separator in the second direction so as to cause the ions to separate.
A multi-reflecting time of flight mass spectrometer (8) comprising: a mass filter or mass separator (6); an ion accelerator (14) for pulsing packets of ions; ion mirrors (10) arranged to receive the ions from the ion accelerator (14) and reflect them in a first dimension (x-dimension) between the ion mirrors (10) as the ions travel in a second dimension (z-dimension); first (20) and second (22) ion reflectors arranged such that when they are both activated they reflect the ions back and forth in the second dimension; an ion detector (16) arranged to receive the ions, when a first of the ion reflectors (20,22) is deactivated; and control circuitry configured to: control the ion accelerator (14) to perform a first pulse sequence that pulses a first plurality of packets of ions into the ion mirrors (10); control the reflectors (20,22) such that ions in said plurality of packets are reflected back and forth in the second dimension by the reflectors (20,22) at the same time; and control the range of mass to charge ratios that is able to be transmitted by the mass filter or mass separator (6) to the ion accelerator (14), and the timings at which the first reflector (20) is activated and deactivated, such that substantially all of the ions from said plurality of packets of ions undergo the same number of reflections in the second dimension before being received at the detector (16).
The present disclosure relates to methods of quantifying a target protein e.g., a tumor biomarker, in a sample by using a mass spectroscopy coupled with a chromatography method. The methods presented herein include incubating the sample with a composition comprising an amidase and a protease for a predetermined amount of time. Incubating the sample with a composition comprising an amidase and a protease in a single step improves the digestion efficiency of the target protein, leading to enhanced detectability e.g., signal to noise ratio, in mass spectroscopy.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
Attorney Docket No.: M-4495-WO01 14 ABSTRACT The present disclosure relates to methods of quantifying a target protein e.g., a tumor biomarker, in a sample by using a mass spectroscopy coupled with a chromatography method. The methods presented herein include incubating the sample with a composition comprising an amidase and a protease for a predetermined amount of time. Incubating the sample with a composition comprising an amidase and a protease in a single step improves the digestion efficiency of the target protein, leading to enhanced detectability e.g., signal to noise ratio, in mass spectroscopy.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
A vial carrier assembly comprising: a base having an opening, the opening having a width and a length; and a holder comprising a least one row of wells, the at least one row of wells of the holder being sized so as to be receivable into the opening across the width of the opening but not receivable into the opening across the length of the opening.
A method of mass and/or ion mobility spectrometry comprising: trapping electrons or reactant ions (3) within a reaction region with DC electric fields; conveying analyte ions (16) into a first side of the reaction region and through the reaction region such that they react with the electrons or reactant ions (3), and allowing the resulting ions (20) to exit the reaction region through a second side of the reaction region that is opposite the first side.
A method of analysing a sample is disclosed in which a solvent emitting capillary is brought into contact with, or proximate to, a sample such that analyte from the sample is absorbed by solvent emitted from the capillary. A voltage is applied to the such that charged droplets of the solvent comprising the analyte from the sample are emitted from the capillary. The charged droplets are caused to be drawn into one or more sampling conduits connected to an atmospheric interface of an analytical instrument.
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
47.
MASS AND/OR MOBILITY SPECTROMETER VACUUM PUMPING LINE
There is provided a mass and/or mobility spectrometer (200) comprising: a vacuum housing (210) having a first vacuum chamber (212) therein; and a first vacuum pump connected to said first vacuum chamber (212) by a conduit (216); wherein at least a part of the conduit (216) extends along the vacuum housing (210) within a wall of the vacuum housing (210).
A method of self-calibrating a mass spectrometer or mass spectral data is disclosed. At least some first observed mass to charge ratios are matched with or against a comprehensive reference set of possible or predicted elemental compositions having known precise mass to charge ratios. One or more calibration parameters of a calibration routine are then adjusted so as to optimise the match between one or more of the first observed mass to charge ratios and the corresponding known precise mass to charge ratios of one or more possible or predicted elemental compositions contained within the reference set.
A start-up routine for a mass spectrometer is performed automatically upon switching ON the mass spectrometer. The mass spectrometer comprises a plurality of functional modules connected thereto, each module operable to perform a predetermined function of the mass spectrometer in use. The start-up routine comprises detecting which functional modules are present in the set of a plurality of functional modules connected to the mass spectrometer, and performing one or more steps of the start-up routine based upon the results of the detection. The mass spectrometer automatically determines whether configuration information is stored locally in respect of each one of the detected functional modules, and, for the or each one of the detected functional modules for which such information is found to be stored locally, automatically uses the information in configuring the mass spectrometer, and, for any detected functional module(s) for which such information is not found to be stored locally, automatically obtains configuration information for the detected functional module(s) from a remote server, and uses the information in configuring the mass spectrometer.
A mass and/or ion mobility spectrometer comprising: an ion source enclosure; a first vacuum chamber in fluid communication with said ion source enclosure via a first orifice; an isolation valve for at least partially closing said first orifice; a first pump for evacuating the ion source enclosure when the isolation valve is closed; and control circuitry configured to operate the spectrometer in a first mode in which the first pump evacuates the ion source enclosure through a conduit having a gas passage therethrough that is relatively restricted, and to then subsequently operate in a second mode in which the first pump evacuates the ion source enclosure through a conduit having a gas passage therethrough that is less restricted.
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A mass filter comprising a plurality of mass filtering electrodes (26a,26b) for mass filtering ions passing therethrough, wherein at least a first of these electrodes (26a) comprises a first gap (34) therethrough. Voltage supplies are arranged and configured to apply voltages to the mass filtering electrodes (26a,26b) such that ions having mass to charge ratios within a mass transmission window are confined by the electrodes and are transmitted through the mass filter, whereas at least some ions having mass to charge ratios outside of said mass transmission window are unstable and pass through the first gap (34) such that they are filtered out by the mass filter. A first collector electrode (36) is arranged so as to receive the ions that are transmitted through the first gap (34), and the voltage supplies apply a DC potential difference between the first mass filtering electrode and the first collector electrode so as to urge the ions that are transmitted through the first gap onto the first collector electrode.
A mass spectrometer comprising: an ion accumulation device (14) for accumulating and pulsing out ions (8); a time of flight mass analyser comprising: ion mirrors (2a, 2b) for reflecting ions; an ion accelerator (4) arranged and configured to receive ions from the ion accumulation device and pulse them into one of the ion mirrors such that ions are reflected back and forth between the mirrors in a first dimension as the ions drift in a drift dimension; reflectors (12a, 12b) for reflecting the ions in the drift dimension; an ion detector (6) for detecting ions; and control circuitry configured to activate the reflectors so as to cause the ions to make multiple passes along the drift dimension and then deactivate one of the reflectors so as to allow ions to pass to the ion detector and be detected, wherein the timings at which the reflectors are activated and deactivated is such that only ions within a first range of mass to charge ratios are capable of having undergone the same number of passes in the drift dimension at the time that they are detected by the ion detector; and wherein control circuitry of the mass spectrometer synchronises the time that ions are pulsed out of the ion accumulation device with the time that ions are pulsed by the ion accelerator such that only ions having mass to charge ratios within said first range are pulsed by the ion accelerator into said one of the ion mirrors.
A nebuliser comprises an outlet aperture and a liquid capillary. A method of operating the nebuliser comprises supplying a gas to the outlet aperture, measuring a flow rate of the gas supplied to the outlet aperture, and determining a position of the liquid capillary relative to the outlet aperture based on the measured flow rate.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
A method of mass analysing a single analytical sample is disclosed comprising: i) transmitting different species of ions through a mass spectrometer; ii) sequentially mass analysing, or otherwise detecting, said different species of ions in a particular sequential order; and then iii) repeating steps i) and ii), wherein the sequential order in which said different species of ions are mass analysed, or otherwise detected, is different when step ii) is repeated. The sensitivity with which the mass spectrometer is able to detect ions varies for a period of time, and step ii) and iii) are performed during that period of time.
A method of mass spectrometry comprising: supplying ions having an initial range of axial kinetic energies towards a mass filter; and mass filtering the ions by applying different voltages to electrodes of the mass filter during different respective dwell times so as to provide different mass transmission windows during the different dwell times; wherein the method comprises increasing the range of axial kinetic energies that the ions have, as they pass towards and/or through the mass filter, during at least one of the dwell times.
A nebuliser outlet (20) comprises one or more first channels (24), wherein the nebuliser outlet (20) is configured such that liquid received by the nebuliser outlet (20) can pass to one or more nebulisation regions via the one or more first channels (24), and one or more second channels (25), wherein the nebuliser outlet (20) is configured such that gas received by the nebuliser outlet (20) can pass to the one or more nebulisation regions via the one or more second channels (25). The one or more first channels (24) comprise a first portion (24a) and a second portion (24b), wherein the first portion (24a) comprises a tubular channel, and wherein the second portion (24b) comprises multiple channels, an annular channel, or a segmented annular channel. The one or more second channels (25) converge with the second portion (24b) at the one or more nebulisation regions.
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A bandpass mass filter comprising: a multipole ion guide; a first RF voltage supply configured to apply a first RF voltage to electrodes of the multipole ion guide for generating an electric field for radially confining ions within the multipole ion guide; and a second RF voltage supply configured to apply a second, different RF voltage to electrodes of the bandpass mass filter, wherein a first phase of the second RF voltage is applied to a first pair of opposing electrodes and a second, different phase of the second RF voltage is applied to a second pair of opposing electrodes; wherein the frequency of the second RF voltage is at least 20 times lower than the frequency of the first RF voltage; and wherein the bandpass mass filter is configured to be maintained at a pressure of ≥ 10-4 mbar.
Power supply systems and methods A power supply system (1) for a mass spectrometer. The system (1) comprises first and second optocouplers (19, 20) which are controlled by a controller (3). The controller (3) is configured to operate in at least one of three control modes to control the optocouplers (19, 20) to deliver a system output signal to a component (7) of a mass spectrometer.
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
H02M 1/00 - Détails d'appareils pour transformation
H03K 17/042 - Modifications pour accélérer la commutation par réaction du circuit de sortie vers le circuit de commande
H03K 17/78 - Commutation ou ouverture de porte électronique, c.-à-d. par d'autres moyens que la fermeture et l'ouverture de contacts caractérisée par l'utilisation de composants spécifiés par l'utilisation, comme éléments actifs, de dispositifs opto-électroniques, c.-à-d. des dispositifs émetteurs de lumière et des dispositifs photo-électriques couplés électriquement ou optiquement
An apparatus is disclosed comprising a first device for generating aerosol, smoke or vapour from one or more regions of a target, an inlet conduit to an ion analyser or mass spectrometer, the inlet conduit having an inlet through which the aerosol, smoke or vapour passes, and a Venturi pump arrangement arranged and adapted to direct the aerosol, smoke or vapour towards the inlet.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A61B 1/04 - Instruments pour procéder à l'examen médical de l'intérieur des cavités ou des conduits du corps par inspection visuelle ou photographique, p. ex. endoscopesDispositions pour l'éclairage dans ces instruments combinés avec des dispositifs photographiques ou de télévision
A61B 1/273 - Instruments pour procéder à l'examen médical de l'intérieur des cavités ou des conduits du corps par inspection visuelle ou photographique, p. ex. endoscopesDispositions pour l'éclairage dans ces instruments pour l'appareil digestif supérieur, p. ex. œsophagoscopes, gastroscopes
A61B 5/00 - Mesure servant à établir un diagnostic Identification des individus
A61B 5/01 - Mesure de la température de parties du corps
A61B 5/0507 - Détection, mesure ou enregistrement pour établir un diagnostic au moyen de courants électriques ou de champs magnétiquesMesure utilisant des micro-ondes ou des ondes radio utilisant des micro-ondes ou des ondes térahertz
A61B 5/055 - Détection, mesure ou enregistrement pour établir un diagnostic au moyen de courants électriques ou de champs magnétiquesMesure utilisant des micro-ondes ou des ondes radio faisant intervenir la résonance magnétique nucléaire [RMN] ou électronique [RME], p. ex. formation d'images par résonance magnétique
A61B 10/00 - Instruments pour le prélèvement d'échantillons corporels à des fins de diagnostic Autres procédés ou instruments pour le diagnostic, p. ex. pour le diagnostic de vaccination ou la détermination du sexe ou de la période d'ovulationInstruments pour gratter la gorge
A61B 10/02 - Instruments pour prélever des échantillons cellulaires ou pour la biopsie
A61B 17/00 - Instruments, dispositifs ou procédés chirurgicaux
A61B 18/00 - Instruments, dispositifs ou procédés chirurgicaux pour transférer des formes non mécaniques d'énergie vers le corps ou à partir de celui-ci
A61B 18/04 - Instruments, dispositifs ou procédés chirurgicaux pour transférer des formes non mécaniques d'énergie vers le corps ou à partir de celui-ci par chauffage
A61B 18/18 - Instruments, dispositifs ou procédés chirurgicaux pour transférer des formes non mécaniques d'énergie vers le corps ou à partir de celui-ci par application de radiations électromagnétiques, p. ex. de micro-ondes
A61B 18/20 - Instruments, dispositifs ou procédés chirurgicaux pour transférer des formes non mécaniques d'énergie vers le corps ou à partir de celui-ci par application de radiations électromagnétiques, p. ex. de micro-ondes en utilisant des lasers
A61B 90/13 - Instruments, outillage ou accessoires spécialement adaptés à la chirurgie ou au diagnostic non couverts par l'un des groupes , p. ex. pour le traitement de la luxation ou pour la protection de bords de blessures pour la chirurgie stéréotaxique, p. ex. système stéréotaxique à cadre avec des guides pour aiguilles ou instruments, p. ex. des glissières courbes ou des articulations à rotule guidés par la lumière, p. ex. pointeurs lasers
A61F 13/38 - Tampons comportant une poignée en forme de bâtonnet, p. ex. bâtonnets ouatés
C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
C12Q 1/04 - Détermination de la présence ou du type de micro-organismeEmploi de milieux sélectifs pour tester des antibiotiques ou des bactéricidesCompositions à cet effet contenant un indicateur chimique
C12Q 1/18 - Test de l'activité antimicrobienne d'un matériau
C12Q 1/24 - Méthodes d'échantillonnage, d'inoculation ou de développement d'un échantillonMéthodes pour isoler physiquement un micro-organisme intact
G01N 1/22 - Dispositifs pour prélever des échantillons à l'état gazeux
G01N 3/00 - Recherche des propriétés mécaniques des matériaux solides par application d'une contrainte mécanique
G01N 9/00 - Recherche du poids spécifique ou de la densité des matériauxAnalyse des matériaux en déterminant le poids spécifique ou la densité
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
G01N 27/624 - Spectrométrie de mobilité ionique différentielle [DMS]Spectrométrie de mobilité ionique à haut champ asymétrique [FAIMS]
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 33/92 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des lipides, p. ex. le cholestérol
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
H01J 49/02 - Spectromètres pour particules ou tubes séparateurs de particules Détails
H01J 49/06 - Dispositifs électronoptiques ou ionoptiques
A rod support for a multipole rod assembly of a mass spectrometer, the rod support comprising a body with an opening extending along a predetermined axis through the body, and two or more rods, which are unitarily formed with the body and extend substantially parallel to the predetermined axis.
There is provided a method of analysis of mass spectrometry data comprising obtaining raw experimental mass spectrometry data; performing a first deconvolution of the raw experimental mass spectrometry data using a deconvolution algorithm, a wide first input parameter set, and a wide first output parameter set to obtain a deconvolved output; obtaining discrete peak data from the deconvolved output; simulating raw data for a first peak of the discrete peak data to obtain reference simulated raw discrete data; simulating raw data for a second peak of the discrete peak data to obtain suspect simulated raw discrete data; and determining whether the second peak is likely an artefact or indicative of a mass by comparing the suspect simulated raw discrete data with the reference simulated raw discrete data.
A mass spectrometer comprising: a vacuum housing comprising a first vacuum chamber having a first gas exhaust port; a gas pump (1700) having a first gas inlet port connected to the first gas exhaust port (H1) by a first gas conduit for evacuating the first vacuum chamber, and a first apertured cover (2010) arranged over the first gas exhaust port (H1) or first gas inlet port, or in the first gas conduit therebetween.
Disclosed herein are various methods and apparatus for performing charge detection mass spectrometry (CDMS). In particular, techniques are disclosed for monitoring a detector signal from a CDMS device to determine how many ions are present in the ion trap (10) of the CDMS device. For example, if no ions are present the measurement can then be terminated early. Similarly, if more than one ion is present, the measurement can be terminated early, or ions can be removed from the trap (10) until only a single ion remains. Techniques are also provided for increasing the probability of there being a single ion in the trap (10). A technique for attenuating an ion beam is also provided.
A method of analysing high-mass (>1 MDa) particles comprises ionising particles to as to produce ions, separating the ions according to mass to charge ratio by passing the ions through an ion separation device in which one or more time-varying electric fields is used to urge ions through a gas such that ions are separated according to mass to charge ratio, measuring the transit time of the ions through the ion separation device, and determining a drift time or mass to charge ratio distribution of the ions therefrom. The method further comprises identifying one or more charge envelopes in the drift time or mass to charge ratio distribution, and using the one or more charge envelopes to characterise the particles.
A method of analysing ions comprises separating ions according to a first physico-chemical property by passing the ions through an ion separation device, and measuring the transit time of an ion through the ion separation device. The method further comprises detecting the ion using a charge-resolving ion detector so as to determine the charge of the ion, and using the transit time and the charge of the ion to determine a second physico-chemical property of the ion.
A nebuliser outlet comprises an inlet end and an outlet end, a first channel and one or more second channels arranged between the inlet end and the outlet end. The first channel is configured to receive a capillary, and the one or more second channels are configured to pass gas to the outlet end. The nebuliser outlet is a single integrated component.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
B33Y 80/00 - Produits obtenus par fabrication additive
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
An electrospray device (8) comprising: a first end (9) for fitting as a cap in, or over, an opening (5) of a tube (2); a second end (10) for electrospraying a liquid sample therefrom; a bore (11) having an entrance opening in the first end and an exit opening in the second end, for allowing liquid to pass into the first end and out of the second end; and an electrode extending from an external surface of the device to a wall of the bore and/or to a surface surrounding the entrance opening. There is also provided a sealed extraction tube containing a liquid solvent for extracting analyte from a swab and electrospraying the analyte.
A charge detection mass spectrometry (CDMS) device comprising: an ion trap for receiving an ion flux and configured for selectively trapping and analysing one or more ions of interest from the ion flux, and at least one primary charge detector, positioned upstream of the ion trap in the path of the ion flux, to analyse the ion flux, wherein the device is configured such that the analysis by the at least one primary charge detector is used to selectively initiate an ion trapping event in the ion trap.
Improved ion mirrors 30 (FIG. 3) are proposed for multi-reflecting TOF MS and electrostatic traps. Minor and controlled variation by means of arranging a localized wedge field structure 35 at the ion retarding region was found to produce major tilt of ion packets time fronts 39. Combining wedge reflecting fields with compensated deflectors is proposed for electrically controlled compensation of local and global misalignments, for improved ion injection and for reversing ion motion in the drift direction. Fine ion optical properties of methods and embodiments are verified in ion optical simulations.
A method for manufacturing a multipole assembly of a mass spectrometer, the method comprising: machining a body to form an opening extending along a predetermined axis through the body, wherein the opening has a profile that defines a pair of opposed seats, each seat being configured to guide a rod of the multipole assembly, cutting the body into a plurality of pieces to form a plurality of identical rod supports, seating a first pair of rods in the seats of a first pair of the rod supports, and seating a second pair of rods in the seats of a second pair of the rod supports, wherein the second pair of rod supports are rotated relative to the first pair of rod supports.
A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; and mass analysing ions.
Improved multi-pass time-of-flight mass spectrometers MPTOF, either multi-reflecting (MR) or multi-turn (MT) TOF are proposed with elongated pulsed converters—either orthogonal accelerator or radially ejecting ion trap. The converter 35 is displaced from the MPTOF s-surface of isochronous ion motion in the orthogonal Y-direction. Long ion packets 38 are pulsed deflected in the transverse Y-direction and brought onto said isochronous trajectory s-surface, this way bypassing said converter. Ion packets are isochronously focused in the drift Z-direction within or immediately after the accelerator, either by isochronous trans-axial lens/wedge 68 or Fresnel lens. The accelerator is improved by the ion beam confinement within an RF quadrupolar field or within spatially alternated DC quadrupolar field. The accelerator improves the duty cycle and/or space charge capacity of MPTOF by an order of magnitude.
A method of mass spectrometry is disclosed comprising: a) providing temporally separated precursor ions; b) mass analyzing separated precursor ions, and/or product ions derived therefrom, during a plurality of sequential acquisition periods, wherein the value of an operational parameter of the spectrometer is varied during the different acquisition periods; c) storing the spectral data obtained in each acquisition period along with its respective value of the operational parameter; d) interrogating the stored spectral data and determining which of the spectral data for a precursor ion or product ions meets a predetermined criterion, and determining the value of the operational parameter that provides this mass spectral data as a target operational parameter value; and e) mass analyzing again the precursor or product ions whilst the operational parameter is set to the target operational parameter value.
An ion mobility separation apparatus comprising: a plurality of ion mobility separator (IMS) devices (12,13) arranged in parallel; an entrance gate (25) configured to direct ions into one or more of said IMS devices at any given time; and control circuitry configured to operate each of the IMS devices in a separation mode in which first voltages are applied to electrodes of the IMS device so as to provide a static DC electric field that urges ions along the IMS device in one direction, and to also apply second voltages to electrodes of the IMS device so as to provide a DC potential that repeatedly travels along the IMS device in the opposite direction such that ions separate according to their mobility within the IMS device.
An instrument for analysing ions is disclosed comprising: a first device (4) configured to onwardly transmit ions having a restricted range of physicochemical property values at any given time, and to change said range with time such that the first device (4) is capable of transmitting ions having different physicochemical property values at different times; and an ion mobility separator (6) arranged to receive ions transmitted by the first device (4); wherein the instrument is configured such that the time that any given ion enters the ion mobility separator (6) and begins to be separated from other ions is defined by its time of transmission by the first device.
A method of separating ions is disclosed comprising: providing an ion separation device comprising a plurality of electrodes; providing a gas flow (5) so as to urge ions in a first direction along the device; applying voltages to said electrodes so that a plurality of travelling potentials (4) urge the ions in a second opposite direction; and varying at least one operational parameter of the travelling potentials (4) as a function of position along the device such that ions of different mobility or mass to charge ratio become trapped at different locations along the device.
A mass spectrometer is disclosed comprising: an ion detector; ion optics for guiding ions to the ion detector; one or more voltage supply for supplying voltages to said ion optics; control circuitry for controlling the one or more voltage supply so as to switch the ion optics between operating in a first mode in which the ion optics are unable to transmit ions having a first mass to charge ratio or first polarity to the ion detector and a second mode in which the ion optics are able to transmit ions having said first mass to charge ratio or first polarity to the ion detector for a time period; and to repeatedly switch between the first and second modes a plurality of times; and a processor and circuitry configured to: (i) determine the intensity of an ion signal detected by the detector at a first time in each of the time periods that the ion optics are in the second mode; and (ii) determine the intensity of the ion signal detected by the detector at a second, later time in each of the time periods that the ion optics are in the second mode.
A method of mass spectrometry comprising: a) accumulating precursor ions in a first ion accumulator; b) performing a separation cycle comprising pulsing a packet of the precursor ions out of the first ion accumulator and into an ion separator, and separating the precursor ions such that precursor ions having different values of a first physicochemical property elute from the ion separator at different times; c) mass filtering the precursor ions that elute from the ion separator so as to transmit a selected precursor ion species; d) fragmenting or reacting the selected precursor ion species so as to generate a set of fragment or product ion species therefrom; e) accumulating the set of fragment or product ion species in a second ion accumulator; f) releasing the set of fragment or product ion species from the second ion accumulator into a TOP mass analyser, wherein operation of the TOP mass analyser is synchronised with the release of ions from the second ion accumulator such that multiple different species of the set of fragment or product ion species are simultaneously pulsed into a time of flight region of the TOP mass analyser by a pusher electrode; and g) repeating steps c) to f) at least once during said separation cycle, wherein said selected precursor ion species is different each time steps c) to f) are performed.
A method of mass spectrometry comprising: a) providing a mass spectrometer having a time of flight (TOP) mass analyser; b) performing a survey scan comprising separating a packet of precursor ion species and mass analysing ions so as to obtain first mass spectral data; c) determining a time window over which one of the precursor ion species, or fragment or product ions derived therefrom, were mass analysed; d) separating another packet of precursor ion species and mass analysing ions so as to obtain second mass spectral data, wherein the pusher of the TOP mass analyser is pulsed according to a plurality of consecutive pulse sequences during a plurality of respective pulse sequence time periods, wherein each pulse sequence consists of consecutive pushes that are arranged such that the duration between any pair of pushes in the pulse sequence is different to the duration between any other pair of pushes within the pulse sequence; f) selecting mass spectral data, from the second mass spectral data, that was obtained during a time period corresponding to said time window of the survey scan, so as to obtain selected data; g) repeating steps d) to f) at least one further time such that multiple sets of said selected data are obtained; combining said multiple sets of selected data and decoding the combined data to obtain mass spectral data representative of the mass to charge ratios of the ions detected by the TOP mass analyser.
A method of analysing ions is disclosed comprising: (i) subjecting ions of an analyte molecule to different activation levels at different times so as to cause the ions to have different mobilities at said different times, wherein the activation level is varied in a plurality of cycles, and wherein the activation level is varied between said different levels during each of the cycles. The method uses an ion mobility separator or scanned ion mobility filter to determine the mobilities of the ions for said different activation levels; and correlates the determined mobilities with their respective activation levels so as to thereby obtain a fingerprint for the analyte molecule.
G01N 27/62 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'ionisation des gaz, p. ex. des aérosolsRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant les décharges électriques, p. ex. l'émission cathodique
A method of determining the enantiomeric purity of an analyte, comprising: ionising the analyte to form dimer ions; separating ions of different dimers of the analyte by ion mobility; detecting a first ion mobility peak corresponding to ions of one or more first dimers and detecting a second ion mobility peak corresponding to ions of one or more second dimers; and determining the enantiomeric purity of said analyte from the ratio of the peak area of the first ion mobility peak to the peak area of the second ion mobility peak.
Provided herein are methods and systems directed to stable, isotopically labeled internal calibrators for use in mass spectrometry analysis for quantifying a target analyte in a sample. The present disclosure relates more particularly to mass spectrometry analysis where a single sample includes at least three isotopically labeled internal calibrators and the target analyte. The methods and systems described herein allows accommodation of isotope interferences arising from the use of isotopically labeled internal calibrators in quantification a target analyte. As a result, smaller quantities (e.g., lesser concentration) of isotopically labeled internal calibrators are utilized in the present technology in the generation of a calibration curve to quantify a target analyte.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
A mass and/or mobility spectrometer comprising: an ion source enclosure for housing an ion source; a vacuum chamber; a pumping block between the ion source enclosure and vacuum chamber; and a shield for protecting a surface of the ion source enclosure; wherein the shield has a first portion configured to mount to a upstream side of the pumping block so as to secure the shield at a location in which a second portion of the shield covers an internal surface of the ion source enclosure.
Provided herein are methods and systems directed to stable, isotopically labeled internal calibrators for use in mass spectrometry analysis for quantifying a target analyte in a sample. The present disclosure relates more particularly to mass spectrometry analysis where a single sample includes at least three isotopically labeled internal calibrators and the target analyte. The methods and systems described herein allows accommodation of isotope interferences arising from the use of isotopically labeled internal calibrators in quantification a target analyte. As a result, smaller quantities (e.g., lesser concentration) of isotopically labeled internal calibrators are utilized in the present technology in the generation of a calibration curve to quantify a target analyte.
Disclosed are methods of mass spectrometry that use charge reduction techniques to reduce the average charge of the ions to increase the mass to charge ratio spacings between different charge states for the ion species prior to mass analysis. In embodiments there is disclosed a method for determining a collision cross section for an ion species, wherein an ion mobility is determined for an ion species in a first state without charge reduction and the mass to charge ratios for the ion species are then measured in a second, charge-reduced state. The average charge determined for the ion species in the first state is then used together with the average mass determined from the ion species in the second state and the ion mobility determined for the ion species in the first state to determine a collision cross section for the ion species in the first state.
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
G01N 27/623 - Spectrométrie de mobilité ionique combinée à la spectrométrie de masse
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
There is provided a method of operating an electrostatic ion trap, the electrostatic ion trap including a plurality of electrodes, the method including setting the voltage of the plurality of electrodes to a first voltage map, wherein the voltage map is configured such that: at a first ion kinetic energy per unit charge (KE) range, more than a first percentage of ions are stable, at a second ion KE range, less than a second percentage of ions are stable, and at a third ion KE range, more than the first percentage of ions are stable, wherein the first percentage is at least 4 times greater than the second percentage, and wherein the second ion KE range is between the first ion KE range and the third ion KE range.
A method comprises measuring a first physico-chemical property of analyte ions so as to produce a data set, and identifying a first group of analyte ions within the data set. Analyte ions within the first group each have a value of an attribute that corresponds to a first value or that is within a first range of the attribute. The method further comprises selecting, from a plurality of different calibrations, a first calibration associated with the first value or first range of the attribute, and calibrating the measured first physico-chemical property of the first group of analyte ions using the first calibration.
An atmospheric pressure ionisation source comprising: an ionisation chamber, comprising an aperture for receiving at least the distal end of a capillary into the ionisation chamber in use, the aperture having a capillary axis; a desolvation heater having a nozzle, for directing a stream of heated gas onto the distal end of the capillary in use, the nozzle having a nozzle axis; a corona discharge device including a corona pin having a corona axis, the corona pin for ionizing a sample in the ionisation chamber in use; and an inlet cone of a mass spectrometer arranged in the ionisation chamber, the inlet cone defining a cone entrance having a cone axis, wherein the cone axis is substantially coaxial with the corona axis and the capillary axis is substantially perpendicular to and intersects with the nozzle axis.
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A temperature monitoring system (16) for use with a thermocouple (21) and a heater controller (24) to control a heater (1). The system (16) comprises a differential amplifier (17) which comprises a first input terminal (18) which is configured to connect to a first terminal (20) of the thermocouple (21) and a second input terminal (19) which is configured to connect to a second terminal (22) of the thermocouple (21). The differential amplifier (17) comprises an output terminal (23) which is configured to provide a temperature sense signal to the heater controller to control the heater (1). The system (16) further comprises a protection circuit (24) which is connected to the first input terminal (18) and the second input terminal (19). The protection circuit (24) is configured to operate in a first mode in the event that a leakage current flows into the first input terminal (18) or the second input terminal (19); and a second mode in the event that a leakage current flows out from the first input terminal (18) or the second input terminal (19), such that the protection circuit (24) controls the differential amplifier (17) to output a temperature sense signal which controls the heater (1) to operate at a temperature at or below a predetermined temperature to reduce the risk of damage being caused by the heater (1).
G05D 23/19 - Commande de la température caractérisée par l'utilisation de moyens électriques
G05D 23/22 - Commande de la température caractérisée par l'utilisation de moyens électriques avec un élément sensible présentant une variation de ses propriétés électriques ou magnétiques avec les changements de température l'élément sensible étant un thermocouple
A guide arrangement for a capillary holder A guide arrangement for a capillary holder of the type having a housing and a capillary protruding from the housing, the guide arrangement comprising: a bracket; and a carriage translatably arranged with respect to the bracket, the carriage having a bed to receive the housing of the capillary holder.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
An atmospheric pressure ionisation source comprising: an ionisation chamber, comprising an inlet for receiving at least the distal end of a capillary into the ionisation chamber in use; a desolvation heater including a heating element, for directing a stream of heated gas onto the distal end of the capillary in use; a corona discharge device arranged in the ionisation chamber; and a control system configured to operate the source in a selected one of: an analytical mode, in which the heating element is heated to a first temperature within a first temperature range, and in which a first current within a first current range is supplied to the corona discharge device; and a capillary priming mode, in which the heating element is heated to a second temperature within a second temperature range, and in which a second current within a second current range is supplied to the corona discharge device, wherein the lower limit of the second temperature range is higher than the lower limit of the first temperature range, and the second current range is higher than the first current range.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
95.
Holder for a capillary triggered to hold when a capillary is inserted
A holder for a capillary, comprising: a housing having a passage to receive a capillary; and a clamping mechanism configurable between: an engaged state in which the clamping mechanism is configured to retain a capillary receivable in the passage in use; and a disengaged state in which the clamping mechanism is configured to allow insertion/removal of a capillary in the passage in use; and a release mechanism operatively associated with the clamping mechanism and movable between a first position, in which the clamping mechanism is configured in said engaged state; and a second position, in which the clamping mechanism is configured in aid disengaged state, wherein the release mechanism is configured to bias the clamping mechanism towards the engaged state, wherein the passage in the housing comprises an end stop, configured to abut the end of a capillary inserted in the passage in use.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A nozzle for directing heated gas onto the distal end of a capillary arrangable adjacent the nozzle, the nozzle comprising: a housing defining a plenum for heated gas; and at outlet comprising at least one aperture fluidly connected to the plenum, the outlet configured to direct a curtain of the heated gas onto the distal end of a capillary in use, such that the curtain of heated gas is substantially aligned with the longitudinal axis of the capillary.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
A method of introducing and ejecting ions from an ion entry/exit device (4) is disclosed. The ion entry/exit device (4) has at least two arrays of electrodes (20,22). The device is operated in a first mode wherein DC potentials are successively applied to successive electrodes of at least one of the electrode arrays ((20,22) in a first direction such that a potential barrier moves along the at least one array in the first direction and drives ions into and/or out of the device in the first direction. The device is also operated in a second mode, wherein DC potentials are successively applied to successive electrodes of at least one of the electrode arrays (20,22) in a second, different direction such that a potential barrier moves along the array in the second direction and drives ions into and/or out of the device in the second direction. The device provides a single, relatively simple device for manipulating ions in multiple directions. For example, the device may be used to load ions into or eject ions from an ion mobility separator in a first direction, and may then be used to cause ions to move through the ion mobility separator in the second direction so as to cause the ions to separate.
Improved pulsed ion sources and pulsed converters are proposed for multi-pass time-of-flight mass spectrometer, either multi-reflecting (MR) or multi-turn (MT) TOF. A wedge electrostatic field 45 is arranged within a region of small ion energy for electronically controlled tilting of ion packets 54 time front. Tilt angle γ of time front 54 is strongly amplified by a post-acceleration in a flat field 48. Electrostatic deflector 30 downstream of the post-acceleration 48 allows denser folding of ion trajectories, whereas the injection mechanism allows for electronically adjustable mutual compensation of the time front tilt angle, i.e. γ=0 for ion packet in location 55, for curvature of ion packets, and for the angular energy dispersion. The arrangement helps bypassing accelerator 40 rims, adjusting ion packets inclination angles α2, and what is most important, compensating for mechanical misalignments of the optical components.
An ion guide assembly (2) is disclosed comprising: two planar mounting components (4); and first and second ion guides (6,8) mounted on the two planar mounting components such that the ion guides are spaced apart from each other, wherein at least one of the planar mounting components has an aperture (14) therethrough that is located between the positions on said at least one mounting component at which the first and second ion guides are mounted; and an ion optical device sized and configured to be inserted through the aperture in the planar mounting component and into the space between the first and second ion guides.
A method of mass and/or ion mobility spectrometry comprising: providing an ion guide comprising a plurality of electrodes and having a background gas therein; applying an RF voltage to electrodes of the ion guide for radially confining ions therein; transmitting clusters of analyte ions and adduct species into the ion guide; applying, in a first mode, one or more AC voltage to the ion guide so as to oscillate the clusters such that they collide with molecules of the background gas and cause adduct species in the clusters to detach from the analyte ions, wherein the one or more AC voltage has a different amplitude and/or frequency to that of said RF voltage; and (i) varying the speed with which the clusters are urged along the ion guide during the first mode; and/or (ii) varying he amplitude and/or frequency of the one or more AC voltage as the clusters travel along the ion guide.
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