Abrégé

A hyaluronidase fusion protein, a yeast engineered strain, and a construction method therefor and a use thereof are provided, belonging to the technical field of genetic engineering. Specifically, a hyaluronidase fusion protein, the amino acid sequence being as shown in SEQ ID NO. 1; a yeast engineered strain, which is obtained by introducing an encoding gene of a hyaluronidase fusion protein into yeast, the nucleotide sequence of the encoding gene being as shown in SEQ ID NO.5 ; the encoding gene sequence of the enzyme of the fusion protein is recombined into the genome of Pichia pastoris to obtain a Pichia pastoris engineered strain. It has been found that the Pichia pastoris engineered strain can effectively express the described fusion protein, and the expressed fusion protein has high enzyme activity and exhibits the activities of two enzymes.

Classes IPC  ?

• C12N 9/26 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons alpha-glucosidiques-1, 4, p. ex. hyaluronidase, invertase, amylase
• C07K 19/00 - Peptides hybrides
• C12N 15/56 - Hydrolases (3) agissant sur les composés glycosyliques (3.2), p. ex. amylase, galactosidase, lysozyme
• C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12N 15/66 - Méthodes générales pour insérer un gène dans un vecteur pour former un vecteur recombinant, utilisant le clivage et la ligatureUtilisation de linkers non fonctionnels ou d'adaptateurs, p. ex. linkers contenant la séquence pour une endonucléase de restriction
• C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 19/26 - Préparation d'hydrates de carbone contenant de l'azote
• C12R 1/84 - Pichia

Produits et services

Eye-washes, not for medical purposes; dentifrices; hand soap; soap; facial cleansers; bath lotion; cosmetics; laundry detergent; essential oils; shoe polish; air fragrancing preparations.

Produits et services

Dentifrices; hand soap; soap; facial cleansers; cosmetics; laundry detergent; essential oils; shoe polish; air fragrancing preparations; bath lotion; eye-washes, not for medical purposes.

Produits et services

Eye-washes, not for medical purposes; dentifrices; non-medicated hand soap; non-medicated beauty soap; facial cleansers; bath lotion; cosmetics; laundry detergent; essential oils; shoe polish; air fragrancing preparations

Abrégé

A joint cavity injection preparation is provided. The active ingredient of the joint cavity injection preparation is deacetylated xanthan gum (XG). The deacetylated XG has a molecular weight of 500,000 to 20,000,000. The joint cavity injection preparation is prepared from deacetylated xanthan gum (XG), which has higher biocompatibility and safe wide-dosage range than existing joint cavity injection preparations prepared from XG.

Classes IPC  ?

• A61K 31/723 - Xanthanes
• A61P 19/02 - Médicaments pour le traitement des troubles du squelette des troubles articulaires, p. ex. arthrites, arthroses
• A61K 31/722 - ChitineChitosane
• A61K 31/728 - Acide hyaluronique
• A61K 31/737 - Polysaccharides sulfatés, p. ex. sulfate de chondroïtine, sulfate de dermatane
• A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier
• A61K 31/7016 - Disaccharides, p. ex. lactose, lactulose

Produits et services

Medicines for human purposes; solutions for contact lenses; biochemical medicines; dietetic substances adapted for medical use; depuratives; medicines for veterinary purposes; preparations for destroying noxious animals; disinfectant wipes; dressings, medical; dental lacquer.

Abrégé

Disclosed is a joint cavity injection preparation and a use thereof. The joint cavity injection preparation uses deacetylated xanthan gum, and has better biocompatibility and a higher safe dosage range compared with existing xanthan gum joint cavity injection preparations.

Classes IPC  ?

• A61K 31/723 - Xanthanes
• A61K 31/728 - Acide hyaluronique
• A61K 9/08 - Solutions
• C08B 37/02 - DextraneSes dérivés
• A61P 19/02 - Médicaments pour le traitement des troubles du squelette des troubles articulaires, p. ex. arthrites, arthroses