A method for separating X and Y sperm cells within a sample sperm cell population, the method including (I) differentiating between and (II) separating sperm cells that have undergone a cellular process and sperm cells that have not undergone the cellular process, whereby a majority of the sperm cells that have undergone the cellular process can comprise one of X or Y sperm cells, and a majority of the sperm cells that have not undergone the cellular process can comprise the other of X or Y sperm cells.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
A61D 19/00 - Instruments ou procédés pour la reproduction ou la fertilisation
A61B 17/43 - Instruments ou procédés de gynécologie ou d'obstétrique pour la reproduction ou la fertilisation pour l'insémination artificielle
A broad object of a the instant invention can be to provide a method for separating X and Y sperm cells within a sample sperm cell population, the method including (i) differentiating between and (ii) separating sperm cells that have undergone a cellular process and sperm cells that have not undergone the cellular process, whereby a majority of the sperm cells that have undergone the cellular process can comprise one of X or Y sperm cells, and a majority of the sperm cells that have not undergone the cellular process can comprise the other of X or Y sperm cells. As to particular embodiments, the cellular process can be a maturational step. As to particular embodiments, the maturational step can be capacitation. As to particular embodiments, the maturational step can be the acrosome reaction. As to particular embodiments, non-viable and viable sperm cells can also be (i) differentiated between and (ii) separated.
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
C12Q 1/24 - Méthodes d'échantillonnage, d'inoculation ou de développement d'un échantillonMéthodes pour isoler physiquement un micro-organisme intact
A broad object of a the instant invention can be to provide a method for separating X and Y sperm cells within a sample sperm cell population, the method including (i) differentiating between and (ii) separating sperm cells that have undergone a cellular process and sperm cells that have not undergone the cellular process, whereby a majority of the sperm cells that have undergone the cellular process can comprise one of X or Y sperm cells, and a majority of the sperm cells that have not undergone the cellular process can comprise the other of X or Y sperm cells. As to particular embodiments, the cellular process can be a maturational step. As to particular embodiments, the maturational step can be capacitation. As to particular embodiments, the maturational step can be the acrosome reaction. As to particular embodiments, non-viable and viable sperm cells can also be (i) differentiated between and (ii) separated.
B03C 1/01 - Prétraitement spécialement adapté à la séparation magnétique par addition d'agents magnétiques
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A process to bulk sex-select sperm which can involve such steps as: obtaining a subject sperm sample such as a collection of cells (1); inducing a sex-based differential alteration process for sperm in the sperm sample; presenting associationally active elements near the sperm within at least some of the sperm sample perhaps such as a fluid combination (4); causing such elements to differentially associate with at least portions of the elements based upon a sperm sex-based differential alteration state; acting on the elements together with their associated sperm through a separation modality (5) to bulk separate the sperm according to their differential sex-based properties. One type of associationally active element is potentially magnetic particles or differentially associatable particles (3) which may even be magnetic particles with inherent zeta potential charges that may associationally act and perhaps bind to an opposite zeta potential or other charges that the sperm may differentially acquire or achieve. This may present a method for magnetic separation of X-bearing and Y-bearing sperm perhaps such as having different charged membranes and perhaps such as after sialic acid activation.
A process to bulk sex-select sperm which can involve such steps as: obtaining a subject sperm sample such as a collection of cells (1); inducing a sex-based differential alteration process for sperm in the sperm sample; presenting associationally active elements near the sperm within at least some of the sperm sample perhaps such as a fluid combination (4); causing such elements to differentially associate with at least portions of the elements based upon a sperm sex-based differential alteration state; acting on the elements together with their associated sperm through a separation modality (5) to bulk separate the sperm according to their differential sex-based properties. One type of associationally active element is potentially magnetic particles or differentially associatable particles (3) which may even be magnetic particles with inherent zeta potential charges that may associationally act and perhaps bind to an opposite zeta potential or other charges that the sperm may differentially acquire or achieve. This may present a method for magnetic separation of X-bearing and Y-bearing sperm perhaps such as having different charged membranes and perhaps such as after sialic acid activation.
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
A61D 19/00 - Instruments ou procédés pour la reproduction ou la fertilisation
A61D 19/02 - Instruments ou procédés pour la reproduction ou la fertilisation pour l'insémination artificielle
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6879 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détermination du sexe
A61K 35/52 - SpermeProstateLiquide séminalCellules de Leydig des testicules
A process to bulk sex-select sperm which can involve such steps as: obtaining a subject sperm sample such as a collection of cells (1); inducing a sex-based differential alteration process for sperm in the sperm sample; presenting associationally active elements near the sperm within at least some of the sperm sample perhaps such as a fluid combination (4); causing such elements to differentially associate with at least portions of the elements based upon a sperm sex-based differential alteration state; acting on the elements together with their associated sperm through a separation modality (5) to bulk separate the sperm according to their differential sex-based properties. One type of associationally active element is potentially magnetic particles or differentially associatable particles (3) which may even be magnetic particles with inherent zeta potential charges that may associationally act and perhaps bind to an opposite zeta potential or other charges that the sperm may differentially acquire or achieve. This may present a method for magnetic separation of X-bearing and Y-bearing sperm perhaps such as having different charged membranes and perhaps such as after sialic acid activation.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6879 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détermination du sexe
7.
METHOD FOR SEPARATING X CHROMOSOME-BEARING SPERM CELLS AND Y CHROMOSOME-BEARING SPERM CELLS
A method for separating X and Y sperm cells within a sample sperm cell population, the method including (I) differentiating between and (II) separating sperm cells that have undergone a cellular process and sperm cells that have not undergone the cellular process, whereby a majority of the sperm cells that have undergone the cellular process can comprise one of X or Y sperm cells, and a majority of the sperm cells that have not undergone the cellular process can comprise the other of X or Y sperm cells.
A61B 17/43 - Instruments ou procédés de gynécologie ou d'obstétrique pour la reproduction ou la fertilisation pour l'insémination artificielle
A61D 19/00 - Instruments ou procédés pour la reproduction ou la fertilisation
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
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