Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
2.
ACOUSTIC SEPARATION FOR HIGH-SPECIFICITY PURIFICATION
A method for separating cells in a biofluid includes pretreating the biofluid by introducing a predetermined amount of a cocktail of antibodies, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the pretreated biofluid within the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of an additive including the cocktail of antibodies, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation is also disclosed. A method of facilitating separation of cells is also disclosed.
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SE (USA)
THE CHARLES STARK DRAPER LABORATORY, INC. (USA)
Inventeur(s)
Glaven, Sarah
Onderko, Elizabeth
Maygar, Andrew
Yates, Matthew
Abrégé
Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.
C02F 3/34 - Traitement biologique de l'eau, des eaux résiduaires ou des eaux d'égout caractérisé par les micro-organismes utilisés
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
A system for cell bioprocessing and cell therapy manufacturing can include a series of microfluidic modules to enable continuous-flow end-to-end cell bioprocessing. Each module can implement a different technology, and the modules can be coupled to one another to perform various unit operations in the cell bioprocessing or cell-therapy manufacturing chain to enable direct processing of a blood or blood product sample. The system can automatically and continuously process the sample into genetically-modified lymphocytes or T cells for cellular therapy. The technologies implemented by each module in the system can include any combination of microfluidic acoustophoresis, microfluidic acoustophoretic media exchange or cell washing, and continuous-flow microfluidic electrotransfection. Modules implementing these microfluidic technologies can be interconnected with plastic tubing or with a custom manifold.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
5.
HAND TOOL WITH INTEGRATED MICROPUMP AND DRUG RESERVOIR FOR INTRACOCHLEAR DRUG DELIVERY
The present disclosure provides a handpiece (100) for trans-canal delivery of a therapeutic substance to the inner ear. The handpiece can be inserted into the middle ear via a surgical tympanotomy approach. The handpiece can be integrated with a micropump (1104) and a fluid reservoir (1106). The handpiece can enable a controlled injection of a therapeutic substance directly through the round window membrane and into the inner ear. The direct delivery of the therapeutic substance to the inner ear can enable the delivery of a precise amount of therapeutic substance into the inner ear. The micropump can include a self-contained fluid reservoir that can provide predetermined volumes of fluid to precise areas of the patient.
A61F 11/00 - Procédés ou dispositifs pour le traitement des oreilles ou de l'ouie Prothèses auditives non électriquesProcédés ou dispositifs permettant au patient d’obtenir une perception auditive par des sens physiologiques autres que l’ouïeDispositifs de protection pour les oreilles, portés sur le corps ou dans la main
6.
HAND TOOL FOR AIDING IN INSERTION OF A TRANS-ROUND WINDOW MEMBRANE CATHETER FOR MICROPUMP-MEDIATED ACUTE AND CHRONIC INNER-EAR DRUG DELIVERY
The present solution provides systems and methods for trans-round window membrane drug delivery. As an overview, a system can include a micropump that is connected to a flexible cannula. The cannula can be threaded through a handpiece that can be used to pierce the round window membrane of a patient. Using the handpiece, the cannula can be inserted through the round window membrane to improve the distribution of the delivered drug throughout the inner ear. The present solution can function as a small implantable or wearable device that can be used for both chronic and acute trans-round window membrane drug delivery. With this configuration, the micropump can constantly or intermittently deliver, over a period of days to months, small volumes of drugs from an internal reservoir.
A61F 11/00 - Procédés ou dispositifs pour le traitement des oreilles ou de l'ouie Prothèses auditives non électriquesProcédés ou dispositifs permettant au patient d’obtenir une perception auditive par des sens physiologiques autres que l’ouïeDispositifs de protection pour les oreilles, portés sur le corps ou dans la main
7.
SYSTEMS AND METHODS FOR MANUFACTURING CLOSED MICROFLUIDIC DEVICES
A method for manufacturing a microfluidic device can include providing a base component to define a first portion of the microfluidic device. A cap component of the microfluidic device can be fabricated with a sealing lip extending a first distance from a first side of the cap component and a support portion extending a second distance, less than the first distance, from the first side of the cap component. The method can include positioning the cap component and the base component within a mold to bring the sealing lip of the cap component in contact with the base component. The base component, the support portion of the cap component, and the sealing lip of the cap component together can define a cavity. The method can include injecting a polymer material into the mold to cause the polymer material to fill the cavity.
MEMS-actuated optical switches can be implemented on photonic chips. These switches are compact, essentially planar, simple to implement and include only one moving MEMS component per switch. The switches exhibit low optical loss, require low power to operate, and are simple to control and easy to integrate with other optical devices. Each switch has two optical waveguides that are optically coupled in an ON switch state and not coupled in an OFF switch state. An end or a medial section of one of the two waveguides may translate between the ON and OFF states to affect the coupling. Alternatively, a coupling frustrator may translate between the ON and OFF states to affect the coupling.
G02B 6/12 - Guides de lumièreDétails de structure de dispositions comprenant des guides de lumière et d'autres éléments optiques, p. ex. des moyens de couplage du type guide d'ondes optiques du genre à circuit intégré
G02B 6/35 - Moyens de couplage optique comportant des moyens de commutation
9.
METHOD AND APPARATUS FOR HIGH THROUGHPUT HIGH EFFICIENCY TRANSFECTION OF CELLS
Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
10.
INTEGRATED MEMS SWITCHES FOR SELECTIVELY COUPLING LIGHT IN AND OUT OF A WAVEGUIDE
A steerable optical transmit and receive terminal includes a MEMS- based Nx1 optical switch network. Each optical switch (1218 - 1226) in the optical switch network uses an electrostatic MEMS structure to selectively position a translatable optical grating (1402) close to or far from an optical waveguide (1410). In the close ("ON") position, light couples between the translatable optical grating (1402) and the optical waveguide (1410), whereas in the far ("OFF") position, no appreciable light couples between the translatable optical grating (1402) and the optical waveguide (1410). The translatable optical grating (1402) is disposed at or near a surface of the optical switch network. Thus, the translatable optical grating emits light into, or receives light from, free space. The steerable optical transmit and receive terminal also includes a lens (2312) and can steer a free space optical beam in a direction determined by which port (2306) of the Nx1 optical switch network is ON.
A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
The present disclosure discussed a handpiece for trans-canal delivery of a therapeutic substance to the inner ear. The handpiece can be inserted into the middle ear via a surgical tympanotomy approach. The handpiece can enable a controlled injection of a therapeutic substance directly through the round window membrane and into the inner ear. The direct delivery of the therapeutic substance to the inner ear can enable the delivery of a precise amount of therapeutic substance into the inner ear. Because the therapeutic substance is delivery directly to the inner ear the delivery of the therapeutic substance is not subject to limitations on molecule size and inconsistent diffusion rates that are present when therapeutic substances are diffused across the round window membrane.
A61M 5/142 - Perfusion sous pression, p. ex. utilisant des pompes
A61M 31/00 - Dispositifs pour l'introduction ou la rétention d'agents, p. ex. de remèdes, dans les cavités du corps
13.
METHOD AND PROCESS FOR QUANTITATIVE EVALUATION OF PHARMACOKINETICS, THERAPEUTIC EFFECTS AND SAFETY OF DELIVERY OF DRUG COMPOUNDS TO THE INNER EAR FOR TREATMENT OF AUDITORY DISEASES
The present disclosure provides compositions and methods for treating an auditory disease in a subject in need thereof comprising administering an effective amount of a gel-based precursor that includes an inner ear-specific therapeutic compound directly into the cochlea of the subject. More particularly, provided is a first pack composition and a second pack composition for use in treating an inner ear auditory disease in a subject in need thereof, wherein the first pack composition comprises about 10 wt.% to about 50 wt.% by weight of a PEG thiol, a PEG thiol¬ ester, or a mixture thereof; and water; and the second pack composition comprises about 10 wt.% to about 50 wt.% by weight of a PEG Michael acceptor, wherein the PEG Michael acceptor is selected from the group consisting of PEG maleimide, PEG vinyl sulfone, PEG acrylate, PEG arylamide, and PEG methacrylate; and water; and the compositions are for administration through a cannula configured to penetrate the round window membrane or for accessing a cochleostomy site or a canalostomy site in the inner ear of the subject.
The present disclosure discusses a system and method that includes a microfluidic device that can be used in either an extracorporeal or implantable configuration. The device supports efficient and safe removal of carbon dioxide from the blood of patients suffering from respiratory disease or injury. The microfluidic device can be a multilayer device that includes gas channels and fluid channels. Distensible membranes within the device can affect a cross-sectional area of the blood channels.
Defects in ferromagnetic materials are detected and characterized by analyzing the items' magnetic fields to find portions of the magnetic fields that differ in characteristic ways from residual magnetic fields generated by non-defective portions of the items. The portions of the magnetic fields that differ in the characteristicways correspond to locations of the defects. The residual magnetic fields correspond to portions of the items distant from the defects. The defect characterizationmay include volume of material lost due to each defect and/or width and/or depth of each defect.
G01N 27/82 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables magnétiques pour rechercher la présence des criques
16.
METHOD FOR REDUCING THE BLOOD PRIMING VOLUME AND MEMBRANE SURFACE AREA IN MICROFLUIDIC LUNG ASSIST DEVICES
A device and method for oxygenating blood is disclosed herein. The device includes a plurality of passive mixing elements that causes a fluid to mix as it flows through the device. The passive mixing elements continually expose new red blood cells to the portion of the flow channel where oxygenation can occur. Accordingly, in some implementations, the device and method uses less blood to prime the device and allows for the oxygenation of blood with a substantial shorter flow channel when compared to conventional oxygenation methods and devices.
ABSTRACTDisclosed herein are systems and devices for culturing cells in a biomimetic environrnent of a cellularized nephron unit, and methods for fabricating and using the cellularized nephron unit. A bioartificial kidney is disclosed that includes a microfluidic flow channel comprising at least one topographical surface, an inlet in fluid connection with the flow channel for allowing fluid to flow into the flow channel; and renal cells seeded on the topographical surface. The topography of the surface of the flow channel may include ridges having a width less than about 5 gm.CA 2839435 2018-11-16
The invention provides systems and methods for exchanging gas in an oxygenator device, and methods for preparing and using such oxygenator devices. The systems and methods can be used to transfer oxygen to blood to assist lung function in a patient.
The invention provides systems and methods for exchanging gas in a microfluidic device, and methods for preparing such microfluidic devices. The systems and methods can be used to transfer oxygen to blood to assist lung function in a patient.
An adhesive article includes a biocompatible and at least partially biodegradable substrate having a surface; anda plurality of protrusions extending from the surface. The protrusions include a biocompatible and at least partially biodegradablematerial, and have an average height of less than approximately 1,000 micrometers.
A61B 17/03 - Instruments, dispositifs ou procédés chirurgicaux pour refermer les plaies ou les maintenir ferméesAccessoires utilisés en liaison avec ces opérations
A61L 15/64 - Utilisation de matériaux caractérisés par leur fonction ou leurs propriétés physiques spécialement adaptés pour être résorbables à l'intérieur du corps
A61L 24/00 - Adhésifs ou ciments chirurgicauxAdhésifs pour dispositifs de colostomie
A61M 37/00 - Autres appareils pour introduire des agents dans le corpsPercutanisation, c.-à-d. introduction de médicaments dans le corps par diffusion à travers la peau
brevets
