System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals. The silicone surfactants of the disclosure are heterografted surfactant block copolymers comprising a polysiloxane backbone, and a plurality of hydrophilic and hydrophobic side chains grafted to said backbone.
Cell-binding agents linked to beads are used to attach the beads to cells that are subsequently partitions. Methods, reaction mixtures, and kits as well as other aspects are provided.
C12N 11/06 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique attachées au support au moyen d'un agent de pontage
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
Systems, methods, and components for automated processing and analyzing a biological sample are disclosed herein. Such components include pre-filled gel cards, gel card cassettes and blotting cartridges for use in automated electrophoresis and protein transfer. Systems includes automated electrophoresis and transfer modules that automatically separate proteins from the biological sample into a plurality of bands and transfer the plurality of bands to a membrane using the gel card cassettes and blotting cartridges herein. Compared to conventional methods, the disclosed system and method provide improvements in reduced experiment time, reduced error, improved precision, and reduced consumption of consumables and chemicals.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
4.
CREATING A VIBRIO-BASED EDUCATIONAL TOOLSET FOR PROTEIN SIGNALING
Described herein are recombinant, attenuated bacteria with an altered quorum sensing pathway. The recombinant bacteria are used for visualizing quorum sensing or immunizing marine organisms against infections by Vibrio spp.
A system and method are provided for predicting a clinical diagnostic laboratory's readiness to meet regulatory proficiency testing (PT) standards, such as the CLIA 2024 regulatory standards. The system obtains laboratory performance data including Lab Mean, Lab Standard Deviation (SD), and Peer Mean for a set of analytes, and models expected result distributions. Using regulatory acceptance limits, the system computes the probability of individual result failure, PT event failure, and regulatory sanction based on binomial models. The system may generate graphical outputs such as distribution overlays and risk contour plots, enabling laboratories to proactively evaluate and improve PT performance. The system may be deployed as a standalone tool or integrated into a laboratory quality management environment.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 10/60 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients
G16H 40/20 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion ou l’administration de ressources ou d’établissements de soins de santé, p. ex. pour la gestion du personnel hospitalier ou de salles d’opération
6.
MIXED MODE CHARGE INDUCTION ION EXCHANGE (IEX) CHROMATOGRAPHY (MMCIIC) LIGANDS AND METHODS OF USE
The subject invention pertains to mixed mode charge induction IEX chromatography (MMCIIC) ligands and chromatography resins suitable for the purification of proteins from biological sources or biological samples. Methods of making mixed mode charge induction IEX chromatography (MMCIIC) matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.
B01D 15/08 - Adsorption sélective, p. ex. chromatographie
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
The invention provides methods for the detection of molecular targets by digital PCR (dPCR) using a set of universal probes and target-specific tailed primers. Each target is amplified by a unique mixture of primers. The tailed amplicons anneal to a universal set of probes to detect the associated targets. Some targets are amplified using more than one tailed primer. Some targets are amplified using the same multiple tailed primers. In these embodiments, the primers are concentrated to produce a different number of amplicons for each tailed primer, resulting in a different probe-amplicon balance for each target. Two colors of fluorescence intensity are read and plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a universal set of probes in multiple assays provides for greater flexibility and throughput.
The present invention relates to novel methods, compositions, kits and systems for the sensitive detection of biomarkers in immunoassays. Disclosed herein are reference materials and methods for monitoring the precision and accuracy of various immunodiagnostic testing methodologies in clinical laboratories.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 33/564 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour complexes immunologiques préexistants ou maladies auto-immunes
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
9.
METHODS, SYSTEMS, AND APPARATUSES FOR GENERATING AND DISPENSING EMULSION DROPLETS
Disclosed herein are novel methods, systems, and apparatuses for generating and dispensing emulsion droplets, which beneficially eliminates the use of microfluidics chips typically used in emulsion droplet generation, thereby reducing the cost and complexity of emulsion droplet generation; and improving the ease of performance, efficacy, and reliability of droplet-based assays. Disclosed herein are high-throughput methods of contacting liquid droplets with an oil to form emulsion droplets, and simultaneously transferring said emulsion droplets to an amplification vessel. Systems and apparatuses suitable to perform the methods of the invention are also provided. Droplets generated accordingly are suitable for a reaction e.g. an amplification reaction, and detection of one or more reaction characteristics or outputs. The method may be particularly useful for the formation of droplets required for amplification reactions such as dPCR.
Compositions for the detection of contaminant enzymes are provided. In particular, oligonucleotide primers are provided, wherein the primer is conjugated to a blocking moiety at its 3'end, wherein the blocking moiety comprises a cleavage site for an enzyme, and wherein cleavage at the cleavage site releases the blocking moiety and produces a 3' hydroxyl group on the oligonucleotide primer. Also provided is an oligonucleotide primer comprising a cleavable moiety at its 5' end, wherein the cleavable moiety is also conjugated to an oligonucleotide tail. Also provided are methods for making the disclosed oligonucleotide primers and methods for using the oligonucleotide primers to detect a contaminant enzyme in a sample.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
A61K 47/54 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique
11.
SINGLE-CELL SEQUENCING USING MULTIPLE TRANSPOSASE ADAPTERS
Methods and compositions are provided for generating cDNA reads from cDNA fragments comprising tagmentation with at least three transposases carrying different homoadaptor oligonucleotides with different end sequences.
Disclosed herein are novel target genomic regions and corresponding primers and probes for performing prenatal testing assays, and methods for performing prenatal testing assays which comprise estimation of foetal fraction, aneuploidy assays, and 22q microdeletion assays, to obtain aneuploidy scores and 22q microdeletion scores, and suitably foetal fraction adjusted aneuploidy scores, and foetal fraction adjusted 22q microdeletion scores. The methods may be particularly useful for NIPT or IPT applications.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/6881 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour le typage de tissu ou de cellule, p. ex. sondes d’antigène leucocytaire humain [HLA]
13.
METHODS AND SYSTEMS FOR TRAINING CLINICAL DIAGNOSTIC MODELS
This disclosure provides a novel approach to training clinical diagnostic models using synthetic data generated by an artificial intelligence (Al) model. The disclosed models demonstrate several advantages, including: reducing time, cost, and complexity associated with traditional data collection and labeling. Additionally, the disclosed models address privacy and data management issues and allow for the inclusion of rare or novel cases in the training set.
G01N 33/80 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir les groupes ou les types sanguins
A61B 5/145 - Mesure des caractéristiques du sang in vivo, p. ex. de la concentration des gaz dans le sang ou de la valeur du pH du sang
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
G16H 10/60 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données spécifiques de patients, p. ex. pour des dossiers électroniques de patients
Methods and compositions are provided for detecting multiple barcodes in a partition for sequencing applications. Bead-specific barcoding oligonucleotides and/or detection oligonucleotides are used to introduce a bead-specific barcode sequence and a universal adapter sequence to target sequences from a single cell or nucleus. Also provided are beads comprising one or more bead-specific barcoding nucleic acids. In addition, the disclosure provides a plurality of partitions that include a single cell or nucleus or nucleic acids from a single cell or nucleus, beads comprising one or more bead-specific barcoding oligonucleotides, and a DNA polymerase.
Disclosed herein are cfDNA-like DNA fragments and broadly applicable methods to prepare cfDNA-like DNA fragments which produce a high yield and/or large quantities of cfDNA-like DNA fragments. The cfDNA-like DNA fragments produced according to the methods of the disclosure accurately capture the size distribution and diagnostic assay performance of natural cfDNA, and may therefore be used for assay methodological validation, internal assay quality control and external assay quality evaluation with high reproducibility and consistency.
An imaging system dynamically computes an offset for an image based on an active region and a reference region of an image sensor used to generate the image. The imaging system applies the offset to pixels of the image. The imaging system further processes the image by performing flat-fielding, binning, or both.
H04N 25/633 - Traitement du bruit, p. ex. détection, correction, réduction ou élimination du bruit appliqué au courant d'obscurité en utilisant des pixels noirs optiques
H04N 25/59 - Commande de la gamme dynamique en commandant la quantité de charge stockable dans le pixel, p. ex. en modifiant le rapport de conversion de charge de la capacité du nœud flottant
H04N 25/76 - Capteurs adressés, p. ex. capteurs MOS ou CMOS
Methods and compositions for method of generating a population of captured target nucleic acids are provided. Exemplary methods can include contacting the plurality of oligonucleotides individually linked to a solid support with a sample comprising target nucleic acids having 3' ends and 5' ends, wherein target nucleic acids anneal to some of the free 3' ends of the oligonucleotides, and wherein there is an excess of oligonucleotides such that at least some oligonucleotides remain with free 3' ends; and then extending the target nucleic acid 3' end with a polymerase using the oligonucleotide as a template to form double-stranded portions that comprise a restriction enzyme recognition sequence; and then cleaving the restriction enzyme recognition sequence with a restriction enzyme to form released target nucleic acids having a single-stranded 5' end and a double-stranded 3' end and leaving oligonucleotides remain with free 3' ends uncleaved from the solid support; and optionally then separating the solid support and uncleaved oligonucleotides from the released target nucleic acids to form a solution of target nucleic acids.
This disclosure provides methods of synthesizing fluorosurfactants and fluorosurfactants produced by the disclosed methods. These fluorosurfactants can be used in various protocols, such as PCR protocols.
Methods and compositions are provided for preparing RNAs, from a single cell, for sequencing applications. Bead-specific barcoding oligonucleotides are used to introduce a beadspecific barcode sequence, a universal adapter sequence, and optionally one or more tail sequences to cDNA from the single cell. Also provided are plurality of partitions that include a single cell or nucleus or nucleic acids from a single cell or nucleus, beads comprising beadspecific barcoding oligonucleotides, a detection oligonucleotide, a reverse transcriptase, and a DNA polymerase.
The invention provides methods for the detection of molecular targets by digital PCR (dPCR) with a blocker for non-target molecules. Each target is provided with a unique mixture of probes. The blocker binds to a non-target molecule and blocks it from contributing to fluorescence from partitions. Two or more colors of fluorescence intensity are read, and two colors of fluorescence intensity are plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a blocker selects against the detection of non-target molecules and improves the resolution of radial multiplexing, allowing multiple targets to be detected.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
21.
DETECTION OF A MODIFICATION TO A POLYNUCLEOTIDE THROUGH PROXIMITY ASSAYS
The subject invention pertains to the detection of modifications to a polynucleotide by binding an affinity binding element to the nucleotide sequence. The affinity binding element can comprise a compound that binds to the modified nucleotide base and a nucleotide sequence that is complementary to the target nucleotide sequence, an oligonucleotide primer that is complementary to the target nucleotide sequence, or a nucleotide sequence complementary to a connector oligonucleotide. The invention provides methods of detecting one or more modifications to a nucleotide sequence. The invention further provides affinity binding elements and, optionally, primer oligonucleotides and/or probe oligonucleotides, and methods of using said affinity binding elements in assays to a detect modified base. The methods of the invention detect a modified nucleotide that are implicated in cancer, psychiatric disorders, or metabolic diseases.
Sample racks and removable partitions removably coupled to the body of the sample rack are provided to stably support sample containers received for sample analysis while maintaining proper alignment of each sample container. A partition can comprise at least two stackable segments, each having gripping flexures disposed to hold a sample container in the desired orientation during use. The sample rack can further include a retaining cap disposed over the partitions to hold them in place during use.
Systems including thermocyclers with dual vapor champers and methods of use of the same are described. The system can be used in performing automated Polymerase Chain Reaction (PCR). The system can include a thermocycler that can thermocycle samples in a PCR cartridge. The thermocycler can include a thermal element, and a first vapor chamber thermally coupled to the thermal element. The first vapor chamber can thermally couple to the PCR cartridge during thermocycling of the PCR cartridge. The system can include an imager and a processor communicatively coupled with each of the thermocycler and the imager. The processor can control the operation of each of the thermocycler and the imager to perform digital PCR. The processor can thermocycle the sample in the PCR cartridge with the thermocycler, and image the sample in the PCR cartridge with the imager.
Methods and compositions for deconvoluting partitions comprising multiple bead-specific barcodes are provided. The methods can involve for example in vitro transcription of detection oligonucleotides within partitions, which can be linked to bead-specific barcodes and sequenced.
Provided herein are methods of detecting fragmentation of an RNA transcript in a sample where the method includes using a first primer pair to amplify a first RNA sequence of the RNA transcript that is at most partially fragmented and a second primer pair to amplify a second RNA sequence of the RNA transcript where all or a portion of the second RNA sequence is fragmented into at least a first RNA fragment and a optionally second RNA fragment.
C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction
This disclosure pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands are also disclosed. Similarly, methods of purifying proteins from a biological sample, source solution, or source liquid using the disclosed chromatography matrices are also provided.
B01J 41/08 - Utilisation d'une substance comme échangeur d'anionsTraitement d'une substance en vue d'améliorer ses propriétés d'échangeur d'anions
B01J 45/00 - Échange d'ions dans lequel se forme un complexe ou un chélateUtilisation d'une substance comme échangeur d'ions formant des complexes ou des chélatesTraitement d'une substance en vue d'améliorer ses propriétés d'échange d'ions formant des complexes ou des chélates
The subject invention pertains to multimodal chromatography resins comprising anionic and cationic ligands on each resin particle and methods of synthesizing the multimodal chromatography resin by contacting a base with quaternary amines and/or tertiary amines on the surface of a substrate. The multimodal resins can be tuned by changing the ratios of cation to anions. The multimodal chromatography resins can be used for purifying proteins.
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01J 20/286 - Phases reliées chimiquement à un substrat, p. ex. à de la silice ou à des polymères
Provided herein is a chromatography media composed of a particulate substrate (such as beads or other particulate substrates) that is coated with hydroxypatite (HA) and in which the particulate substrate used as the base upon which calcium phosphate (CaP) is formed is treated with heat to remove the substrate, convert the CaP to HA, and form the desired HA microstructure so as to form a templated porous HA substrate (TPHA substrate). Also provided is a choromatography media (optionally contained within a column) comprising a TPHA substrate (e.g., a plurality of TPHA particles). The TPHA particles are porous or macroporous and uses thereof..
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
B01J 20/06 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des oxydes ou des hydroxydes des métaux non prévus dans le groupe
This disclosure relates to particulate hydroxyapatite (HA)-coated substrates, methods of making particulate HA-coated substrates, and methods of using particulate HA-coated substrates. Thus, the disclosure provides chromatography media composed of a particulate substrate (such as polymer beads or other particulate substrates) that is coated with HA. Also provided is a chromatography media, (optionally contained within a column) comprising a HA-coated substrate (e.g., a plurality of beads or another particulate substrate that is coated with HA). The beads or particulate substrate can be a porous or non-porous substrate.
B01J 20/04 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant des composés des métaux alcalins, des métaux alcalino-terreux ou du magnésium
A61L 27/32 - Matériaux contenant du phosphore, p. ex. apatite
C01B 25/32 - Phosphates de magnésium, de calcium, de strontium ou de baryum
C04B 28/34 - Compositions pour mortiers, béton ou pierre artificielle, contenant des liants inorganiques ou contenant le produit de réaction d'un liant inorganique et d'un liant organique, p. ex. contenant des ciments de polycarboxylates contenant des liants phosphate froids
Provided herein is a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with hydroxyapatite ( HA ) Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Thus this disclosure provides a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with HA. Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Methods of preparing the HA-coated substrate are also provided.
Compositions and methods for fixing cells or nuclei or extracellular vesicles with dithiobismaleimidoethane (DTME) and subsequent reverse transcription in the cells or nuclei or extracellular vesicles are provided.
C40B 40/02 - Bibliothèques contenues ou présentées dans des micro-organismes, p. ex. des bactéries ou des cellules animalesBibliothèques contenues ou présentées dans des vecteurs, p. ex. des plasmidesBibliothèques contenant uniquement des micro-organismes ou des vecteurs
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
A probe cleaning system and method including multiple pressurized sources and a valve that are configured for cleaning a probe. The valve includes multiple inlet channels, an outlet channel, and a selector. Each inlet channel can be connected to a specified pressurized cleaning source of the multiple pressurized cleaning sources. The plurality of inlet channels are isolated from each other to avoid cross-contamination. The selector can be configured to open a specified inlet channel to pass one specified pressurized cleaning source to the outlet channel at a specified time. The probe can be coupled to the outlet channel of the valve to receive the specified pressurized cleaning source when the specified inlet channel of the valve is opened during a cleaning cycle.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
A61B 1/00 - Instruments pour procéder à l'examen médical de l'intérieur des cavités ou des conduits du corps par inspection visuelle ou photographique, p. ex. endoscopesDispositions pour l'éclairage dans ces instruments
A61B 1/12 - Instruments pour procéder à l'examen médical de l'intérieur des cavités ou des conduits du corps par inspection visuelle ou photographique, p. ex. endoscopesDispositions pour l'éclairage dans ces instruments avec système de refroidissement ou de rinçage
A61B 90/70 - Dispositifs de nettoyage spécialement adaptés aux instruments chirurgicaux
A61L 2/00 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet
A61L 2/16 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des substances chimiques
A61L 2/18 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des substances chimiques des substances liquides
A61L 2/20 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des substances chimiques des substances gazeuses, p. ex. des vapeurs
Methods and compositions for nucleotide sequencing are provided. In some embodiments, click chemistry is used to link barcoding oligonucleotides to DNA fragments comprising adapters introduced by a transposase.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
The present relates to chromatic focusing via filter thickness tuning. Chromatic focusing can be achieved via an imaging system that includes a sample holding plane, a light source that illuminates the sample on the plane, and an imager that captures an image of the sample on the plane. The imager includes a sensor, a lens, and a filter wheel defining a plurality of filter apertures including a first aperture containing a first filter passing light of a first color and having a first focal point shift, and a second aperture containing a second filter passing light of a second color and having a second focal point shift. The focal point shifts cause both light of the first color passing through the first filter and through the lens and light of the second color passing through the second filter and through the lens to focus on the sensor.
Systems, methods, and devices for imaging of stain-free fluorescence on western blot membranes with excitation by epi illumination with UV LEDs are disclosed herein. A method can include collecting and preparing a sample, separating the sample via gel electrophoresis in a gel block, transferring the separated sample from the gel block to an analysis block, generating an image of the sample with an imager, and evaluating the image of the sample. The imager can include a plane to receive and hold a block containing a sample and including a first side and a second side, a camera to image a sample on the plane and positioned above the first side of the plane, and an LED light source positioned above the first side of the plane. The LED light source can illuminate the sample on the plane via epi-illumination, and emits light having a wavelength in a range from approximately 325 nm to approximately 400 nm.
Provided is a heterohybridoma-based method of generating recombinant rabbit monoclonal antibodies, recombinant anti-IL-6 receptor-alpha (IL-6Rα) antibodies generated using such methods, and immune assay methods kits employing such antibodies.
C07K 16/24 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des cytokines, des lymphokines ou des interférons
39.
METHODS AND COMPOSITIONS FOR TRACKING BARCODES IN PARTITIONS
Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.
B01J 8/08 - Procédés chimiques ou physiques en général, conduits en présence de fluides et de particules solidesAppareillage pour de tels procédés avec des particules mobiles
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C40B 20/04 - Identification des éléments d'une bibliothèque au moyen d'une étiquette, d'un marqueur ou d'un autre identificateur lisible ou détectable, p. ex. procédés de décodage
C40B 40/08 - Bibliothèques comprenant de l'ARN ou de l'ADN codant des protéines, p. ex. bibliothèques de gènes
40.
UNIVERSAL FLUORESCENT PROBES ACTIVATED WITH RNASEH2
Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/6853 - Réactions d’amplification d’acides nucléiques utilisant des amorces ou des matrices modifiées
C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
41.
METHOD FOR ESTIMATION OF FETAL FRACTION IN CELL-FREE DNA FROM MATERNAL SAMPLE
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/6881 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour le typage de tissu ou de cellule, p. ex. sondes d’antigène leucocytaire humain [HLA]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
42.
VARIANT CLASSIFICATION THROUGH HIGH-CONFIDENCE MUTATION DETECTION FROM FLUORESCENCE SIGNALS MEASURED WITH A MULTIPLE MUTATION ASSAY
A system and method for SARS-CoV-2 variant classification through mutation detection from qPCR fluorescence signals. The system receives fluorescence signals, wherein a first fluorescence signal indicates quantitative presence of a first genomic region as a control for SARS-CoV-2, and a second fluorescence signal indicates quantitative presence of a second genomic region of a first mutation present in a subset of variants of SARS-CoV- 2. The system measures a first Cq for the first fluorescence signal and a second Cq for the second fluorescence signal as the signals cross a threshold RFU. The system calculates a delta. Cq as a difference between the two. Further, the system identifies a first peak RFU for the first fluorescence signal and a second peak RFU for the second fluorescence signal, and calculates a RFU ratio of the two. The system detects presence of the first mutation based on the delta Cq and/or the RFU ratio.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
An instrument (e.g., a thermal cycler) includes a consumable compartment configured to receive multiple height consumables, and a lid for opening or closing the consumable compartment. The lid includes a motor, a pressure plate, a compression bar, and a cam and a follower. The compression bar has a first side (e.g., bottom) coupled to the pressure plate and a second side (e.g., top) at which the cam and the follower is disposed. The cam is drivably coupled to the motor and the follower is coupled to the compression bar to induce translation of the compression bar when engaged with the cam. The cam includes a cam profile configured to adjust a height of the pressure plate between different height consumables to apply a pre-determined pressure. The motor can also operate a latch assembly to latch the lid before activating the cam and follower assembly.
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
44.
SYSTEM AND METHOD FOR DESIGNING QUALITY CONTROL (QC) RANGES FOR MULTIPLE CLINICAL DIAGNOSTIC INSTRUMENTS TESTING THE SAME ANALYTE
Systems and methods for performing testing of a single analyte on a group of multiple clinical diagnostic analyzers, or a single clinical diagnostic analyzer having multiple analytic units, or combinations thereof, are disclosed. A mean and SD for each individual instrument are input to at least one of the instruments along with a QC rule to be used, the probability of false rejection function for the QC rule, and a desired false rejection rate. A group mean and a group SD are calculated to satisfy the desired false rejection rate and QC rule and loaded into each individual instrument for use in testing the single analyte at each individual instrument.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G16H 40/40 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion d’équipement ou de dispositifs médicaux, p. ex. pour planifier la maintenance ou les mises à jour
G16H 40/63 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement local
45.
SYSTEM AND METHOD FOR CUSTOMIZED AUTOMATED CLINICAL DIAGNOSTIC CROSSOVER STUDIES
Systems and methods for conducting customized automated crossover studies for clinical diagnostic analyzers are disclosed. In disclosed embodiments, an optimal number of samples to run on a new QC material is determined based on historical information of the analyzer instrument and a peer group of similar instruments. Using the determined optimal number of samples, the crossover study is conducted, and the results are reported.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G16H 40/63 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement local
Systems, including methods and apparatus, for analyzing partitioned samples, such as droplets, using recirculated fluid. The systems may be used to analyze a plurality of partitioned samples, with fluid used with initial samples reused with later samples. This reuse, or recirculation, may reduce the amount of fluid required for performing multiple analyses, with concomitant reductions in costs. Moreover, in some embodiments, it may simplify operation by increasing the number of analyses that may be performed before fluid must be replenished or replaced.
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
Methods of decreasing bead aggregation and improving specificity of nucleic acid hybridization using non-specific nucleic acid binding proteins is described.
One or more instruments generate test result data. The test result data include patient data and QC data. The test result data is provided to a QC data flow system via a local network, which filters the test result data (e.g., using a set of rules) to extract the QC data. The QC data is provided to a cloud-based QC data management platform via an external network. The cloud-based QC data management platform analyzes the QC data and provides a result back to the QC data flow system. The QC data flow system forwards the result to middleware or the instrument, which triggers a corrective action based on the result as appropriate.
G16H 40/40 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion d’équipement ou de dispositifs médicaux, p. ex. pour planifier la maintenance ou les mises à jour
G06Q 10/06 - Ressources, gestion de tâches, des ressources humaines ou de projetsPlanification d’entreprise ou d’organisationModélisation d’entreprise ou d’organisation
G06F 15/16 - Associations de plusieurs calculateurs numériques comportant chacun au moins une unité arithmétique, une unité programme et un registre, p. ex. pour le traitement simultané de plusieurs programmes
49.
METHOD AND SYSTEM FOR DETECTING REVERSE TRANSCRIPTASE ACTIVITY BY DIGITAL ASSAY
Method and system for detecting reverse transcriptase activity of a sample by digital assay. In an exemplary method, a reaction mixture including the sample, an RNA polymer, and reagents for reverse transcription may be prepared. Complementary DNA (cDNA) may be synthesized in the reaction mixture using the RNA polymer as a template. An amount of the cDNA synthesized may be proportional to the reverse transcriptase activity of the sample. Partitions may be formed after synthesizing the cDNA. The partitions may contain copies of the cDNA at partial occupancy. Each partition may include a portion of the reaction mixture. A target representing the cDNA may be amplified in the partitions. Amplification data for the target may be collected from the partitions.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
50.
METHODS AND HYDROGEL COMPOSITIONS FOR PARTITIONING BIOLOGICAL SAMPLES
Methods of making and forming partitions in nanovials are provided. Wherein linking oligonucleotides to cell nucleic acids in hollow nanovials, the method comprising, loading cells into openings in hollow nanovials; inserting one or more hydrogel bead into the openings, thereby occluding the openings and preventing diffusion the cells from the openings is disclosed.
Systems and methods for automated digital polymerase chain reaction are described herein. A method of performing automated digital Polymerase Chain Reaction (PCR) can include receiving a sample in a sample tube within a sample tube holder of an automated digital PCR system. The automated digital PCR system can include a multichannel pipettor, a heater that can thermocycle samples in a PCR cartridge, and an imager. The method can include performing pipetting operations with the multi-channel pipettor to transfer a portion of the sample to a PCR cartridge, thermocycling the sample in the PCR cartridge with the heater, and imaging the sample in the PCR cartridge with the imager.
Methods and systems for detecting particles. In an exemplary method, a sample fluid including the particles may be driven from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis. Focusing fluid may be introduced into the confluence region from at least two focusing channels along respective introduction axes. Introducing may be rotationally asymmetrical about the longitudinal axis. The introduction axes and the longitudinal axis may collectively extend in three dimensions. The particles may be passed through an interrogation zone of the sample outlet channel. The interrogation zone may be irradiated with light. Optical radiation may be detected from the interrogation zone.
G01N 33/557 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant des mesures cinétiques, c.-à-d. mesure de l'évolution en fonction du temps de l'interaction antigène-anticorps
G01N 33/536 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
Methods, compositions, and kits for analyzing viral particles. In an exemplary method of analyzing viral particles, capsids of the viral particles may be tagged with a tag. Subsamples of a sample containing the viral particles may be formed. Each subsample of only a subset of the subsamples may include at least one of the viral particles. One or more targets may be amplified from a genome of the viral particles. Tag-related data, and amplification data for the one or more targets, may be collected from the subsamples. In some examples, a capsid occupancy of the viral particles may be determined in a calibration-free approach using the collected data without quantification of the viral particles or capsids thereof.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12N 7/00 - Virus, p. ex. bactériophagesCompositions les contenantLeur préparation ou purification
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
55.
METHOD AND SYSTEM FOR SIZING A POPULATION OF NUCLEIC ACID FRAGMENTS USING A DIGITAL ASSAY
Methods, systems, and computer-readable media for sizing a population of nucleic acid fragments. In an exemplary method of sizing a population of nucleic acid fragments provided by a sample, partitions may be formed, each containing a portion of the sample. Each target of at least two targets may be present in only a fraction of the nucleic acid fragments. Amplification reactions may be performed for each of the at least two targets in the partitions. Amplification data may be collected for the at least two targets from the partitions. Target-defined subsets of the partitions may be enumerated using the amplification data. At least one length characteristic of the population of nucleic acid fragments may be predicted using results of enumerating.
The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.
Disclosed is a system and method for dynamically adjusting analytical precision of a clinical diagnostic process. The system and method employ a clinical diagnostic analyzer that evaluates a sample and determines whether the resultant value, or mean value, is within a predetermined window or within a threshold of a predetermined value of a precision profile. If so, the analyzer automatically and dynamically determines a desired analytical precision and conducts additional testing of replicate samples to achieve a desired precision and reports the results to a user.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
G16H 15/00 - TIC spécialement adaptées aux rapports médicaux, p. ex. leur création ou leur transmission
G16H 40/40 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion d’équipement ou de dispositifs médicaux, p. ex. pour planifier la maintenance ou les mises à jour
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
A wide-spectrum analysis system. The system may comprise various components, including a stage, a detection module, and an optical relay structure. The stage may be configured to support a sample holder—a gel or blot, a PCR plate or microplate, sample chips, or a microfluidic device, among others—at an examination region. The detection module may be configured to detect light originating from one or more samples positioned in the sample holder. The detection module may be configured to detect light having wavelengths between about 200 nm and about 2000 nm, or subsets thereof. The optical relay structure may be configured to direct the output light from the examination region to the detection module. The system may further comprise an illumination module. Embodiments of the analyzer may be suitable for use with one or more of the following interrogation formats, among others: chemiluminescence, fluorescence, colorimetry, and spectrometry.
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
G02B 21/26 - PlatinesMoyens de réglage pour celles-ci
G01N 33/72 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir les pigments du sang, p. ex. l'hémoglobine, la bilirubine
G01J 3/10 - Aménagements de sources lumineuses spécialement adaptées à la spectrométrie ou à la colorimétrie
59.
COMPUTER VISION FOR REAL-TIME SEGMENTATION OF FLUORESCENT BIOLOGICAL IMAGES
A segmentation system processes images of a sample that includes fluorescent entities. The segmentation system applies a threshold image that represents background illumination distinct from fluorescence of target entities to pre-process the images. The segmentation system determines numbers of target entities in pre-processed images and determines whether an estimated number of target entities in the sample meets a threshold certainty. The segmentation system continues to analyze one or more images until the threshold certainty is determined. When the threshold certainty is met, the estimated number of target entities may be used to generate a user interface output (e.g., displaying the pre-processed images and visual indicators of the locations of target entities).
Systems and methods for contact imaging of stands with illumination are disclosed herein. A contact imaging system can include a contact imager. The contact imager can include a housing having a base and a lid. The lid can have a closed position against the base and an open position. The contact imager can include a contact area image sensor. The lid can shield the contact area image sensor from ambient light when the lid is in the closed position. The contact imager can include an illuminator that can illuminate at least a portion of a blot on the contact area image sensor when the lid is in the closed position.
Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6832 - Amélioration de la réaction d’hybridation
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction
62.
COMPOSITIONS, METHODS, AND SYSTEMS FOR SAMPLE PROCESSING WITH MORPHOLOGY-ADJUSTABLE FUNCTIONALIZED PARTICLES
Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.
B01J 8/00 - Procédés chimiques ou physiques en général, conduits en présence de fluides et de particules solidesAppareillage pour de tels procédés
B01J 8/02 - Procédés chimiques ou physiques en général, conduits en présence de fluides et de particules solidesAppareillage pour de tels procédés avec des particules immobiles, p. ex. dans des lits fixes
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
64.
METHODS AND COMPOSITIONS FOR GENOTYPING AND PHENOTYPING CELLS
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12N 5/02 - Propagation de cellules individuelles ou de cellules en suspensionLeur conservationMilieux de culture à cet effet
A fluorescence detection system, including apparatus and methods, suitable for qPCR and other fluorescence-based analyses. The system may comprise various components, including a stage, an illumination module, a detection module, and an optical relay structure. The stage may be configured to support a sample holder. The illumination module may include one or more discrete light sources configured to produce excitation light. The detection module may be configured to detect fluorescence emission light produced, in response to the excitation light, by a fluorescent sample positioned in the sample holder. The optical relay structure may include a beamsplitter assembly configured to direct the excitation light from the illumination module along an illumination path to the sample holder and to direct the fluorescence emission light from the sample holder along a response path to the imaging module. The system may enhance the quality of excitation light hitting samples in the sample holder, for example, by collimating and/or homogenizing the light.
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
G01N 21/25 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
The subject invention pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.
C07K 1/16 - ExtractionSéparationPurification par chromatographie
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
This disclosure pertains to a consumable product (microplate) suitable for use in assays utilizing single-molecule recognition through equilibrium Poisson sampling (SiMREPS) and in other assays employing total internal reflection fluorescence (TIRF) or HiLo microscopy. The disclosed microplate is also suitable for use in other high throughput assay systems, such as single-molecule FRET, ligand-receptor binding studies, membrane biology assays, cell-based TIRF and near- TIRF assays. The disclosure further pertains to the use of the microfluidic microplate for the detection of analytes, including nucleic acids, polypeptides, carbohydrates, lipids, post-translational modifications, amino acids, metabolites, and small molecules.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
Tagging biological substrates with molecular barcodes in partitions can provide novel biological insight of the substrates that co-localize to discrete partitions, through the sequencing of the molecular barcodes and analysis, thereof. Methods and compositions for determining the proximity of two barcoding oligonucleotides (e.g., in a single partition or adjacent on a tissue section) using a determination of the presence of a 9 bp sequence resulting from tagmentation in different nucleic acid fragments linked to different barcoding oligonucleotides is provided.
Aspects of the present disclosure relate to systems and methods for generating a composite image. This can include a western blot imager with a real time camera. One aspect of the present disclosure relates to an imaging system. The imaging system includes a sample plane that can receive and hold a sample, a photon resolving camera, and a lens attached to the photon resolving camera, the photon resolving camera and the lens positioned to image the sample plane.
H04N 23/10 - Caméras ou modules de caméras comprenant des capteurs d'images électroniquesLeur commande pour générer des signaux d'image à partir de différentes longueurs d'onde
G16B 20/00 - TIC spécialement adaptées à la génomique ou protéomique fonctionnelle, p. ex. corrélations génotype-phénotype
70.
CIRCUIT FOR SHARING CURRENT BETWEEN PARALLEL LEDS OR PARALLEL STRINGS OF LEDS
A circuit for sharing current between parallel LEDs or parallel strings of LEDs, and a method of use of the same, are disclosed herein. The circuit for sharing current between parallel LED pathways can include a first LED pathway, a first transistor coupled to the first set of LEDs and that can control a first current through the first set of LEDs, and a first measurement node having a first sensed voltage. The circuit can include a second LED pathway, a second transistor coupled to the second set of LEDs and that can control a second current through the second set of LEDs, and a second measurement node having a second sensed voltage. The circuit includes a first differential amplifier and a second differential amplifier, each of which can compare sensed voltage and can apply a voltage to a gate of one of the first and second transistors.
The subject invention pertains to the detection and differentiation of genetic variations by nucleic acid amplification. The invention provides methods of detecting one or more genetic variations in a nucleic acid that are in close proximity simultaneously. The invention further provides primer and probe oligonucleotides and methods of using said primers and probes in assays to detect genetic variants of concern of SARS-CoV-2. The methods of the invention detect genetic variants of other pathogens, including influenza, or genetic variants involved in inheritable diseases or cancer.
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
A61K 39/42 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire viraux
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
The present disclosure relates to the development of novel immunoassays for the detection of SARS-CoV-2 or secreted spike protein (or fragments thereof) in saliva, nasal mucosal sample, throat samples, or nasopharyngeal samples.
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
73.
DYNAMIC AXIAL COMPRESSION FOR PREPARATIVE COLUMNS USING EXTERNAL COMPRESSION
A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
B01D 15/08 - Adsorption sélective, p. ex. chromatographie
The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic l,3-droxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.
C07D 241/12 - Composés hétérocycliques contenant des cycles diazine-1,4 ou diazine-1,4 hydrogéné non condensés avec d'autres cycles comportant trois liaisons doubles entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec uniquement des atomes d'hydrogène, des radicaux hydrocarbonés ou des radicaux hydrocarbonés substitués, liés directement aux atomes de carbone du cycle
Methods and compositions for characterizing a biological sample (e.g., comprising an infectious agent) from a subject are provided. Methods can include detecting linkage of nucleic acids that are linked in a viable cell or organism but that become degraded and thus unlinked in inviable cells or organisms and then characterizing the subject based on the quantity of linked and unlinked sequences.
In one implementation, a microfluidic probe has a non-planar processing surface and an inlet aperture. The shape of the surface may be selected to produce a specific velocity gradient profile across a surface onto which fluid is deposited using the microfluidic probe, for example a constant velocity gradient or a velocity gradient that decreases linearly with distance from the inlet aperture. The microfluidic probe may define and overflow notch in a perimeter edge of the processing surface.
A method comprises providing a microfluidic device including a reservoir; an injection nozzle at the bottom of the reservoir, the injection nozzle being wider at its top than at its bottom; an injection channel below the injection nozzle; and a microfluidic channel below the injection channel. The method further comprises placing fluid in the reservoir, and allowing the fluid to passively flow through the injection nozzle and the injection channel.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 99/00 - Matière non prévue dans les autres groupes de la présente sous-classe
G01N 1/28 - Préparation d'échantillons pour l'analyse
G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
78.
SYSTEM AND METHOD FOR CONDUCTING AUTOMATED CLINICAL DIAGNOSTIC CROSSOVER STUDIES
A clinical diagnostic analyzer for performing an automated crossover study on quality control (QC) material includes a processor, memory, measurement hardware, and an input panel/display. The analyzer prompts a user to load a QC specimen, and to instigate testing and analysis to determine a mean and a standard deviation for the new material. Associated methods for using one or more clinical diagnostic analyzers to calculate a new mean and standard deviation for a new QC material, reduce error in the calculated mean value, and to reduce the total number of days to complete a crossover study are also disclosed.
A clinical diagnostic analyzer for performing a virtual crossover study on quality control (QC) material includes a processor, memory, measurement hardware, and an input panel/display. The analyzer acquires data from a peer group relating to a new lot of quality control material to calculate a predicted mean and standard deviation based on that peer group data and adjusted for bias in the laboratory process. As new analyses are run on the new lot of QC material, the predicted mean and standard deviation are updated to incorporate the actual data on a weighted basis.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicalesTIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p. ex. basé sur des systèmes experts médicaux
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 1/00 - ÉchantillonnagePréparation des éprouvettes pour la recherche
80.
METHOD AND COMPOSITION FOR QUANTIFYING PROTEIN USING TAGGED STANDARDS
Methods and reference compositions for quantifying protein using tagged standards. In an exemplary method, a reference composition and a protein may be electrophoresed in respective lanes of a gel. The reference composition may include quantitation standards of different size and each including a tag present at a different concentration. The quantitation standards and the protein may be transferred from the gel to a solid support to create a blot. Luminescence may be detected from the blot to obtain respective luminescence values separately representing an abundance of the tag of each quantitation standard and an abundance of the protein. A quantity of the protein may be determined using the respective luminescence values.
G01N 33/542 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec inhibition stérique ou modification du signal, p. ex. extinction de fluorescence
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
82.
SYSTEM AND METHOD FOR RAPID MULTIPLEXED SAMPLE PROCESSING WITH APPLICATIONS FOR NUCLEIC ACID AMPLIFICATION ASSAYS
The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.
A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.
The subject invention pertains to compositions of novel analogs of red blood cells that are distinguishable from white blood cells in a hematological instrument and processes for manufacturing such analogs. The processes for creating the compositions comprise washing, shrinking, and fixing cells at temperatures at or below room temperature.
G01N 33/48 - Matériau biologique, p. ex. sang, urineHémocytomètres
G01N 33/96 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir un étalon de contrôle du sang ou du sérum
A computer system detects that an external update device (e.g., a USB drive) is connected and adds an update script to its startup event. When the computer system is starting up, it checks to confirm that the external update device is still connected and, if so, runs the update script. The update script executes an update installer on the external update device. The update installer generates a list of software updates to install and installs them using a system account of the computer system. The update installer may restart the computer system if needed to complete installation of the updates.
G06F 21/57 - Certification ou préservation de plates-formes informatiques fiables, p. ex. démarrages ou arrêts sécurisés, suivis de version, contrôles de logiciel système, mises à jour sécurisées ou évaluation de vulnérabilité
86.
ELECTROPHORESIS APPARATUS WITH MINIMAL AUTOFLUORESCENCE TO ENABLE GEL PROCESSING IN SITU
An electrophoresis apparatus with minimal autofluorescence to enable gel processing in situ, and methods of making and using the electrophoresis apparatus. An exemplary electrophoresis apparatus may comprise a cassette defining a cavity between a first pane and a second pane of a double-paned viewing window, and also may comprise a slab gel located in the cavity. The viewing window may be transparent to ultraviolet light that drives a derivatization reaction in the slab gel, and, absent the slab gel, may define a window autofluorescence inducible by irradiation with the ultraviolet light. The window autofluorescence, per unit area, may be less than five-fold a gel autofluorescence of the slab gel, per the same unit area and under the same irradiation with the ultraviolet light.
The present invention relates to the development of novel immunoassays for the detection of neutralizing antibodies and/or high avidity neutralizing antibodies to SARS- CoV -2 spike protein variants or fragments thereof and. optionally, one or more cytokine in patient samples. Novel multiplex and singieplex immunoassays for the detection of neutralizing antibodies and/or high avidity neutralizing antibodies to SARS-CoV-2 spike protein variants or fragments thereof and, optionally, one or more cytokine in patient samples are also provided.
Barcoding composition and methods involving solid supports having sets of different oligonucleotides that can be decoded to the same identification sequence.
C12Q 1/34 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase
C40B 30/04 - Procédés de criblage des bibliothèques en mesurant l'aptitude spécifique à se lier à une molécule cible, p. ex. liaison anticorps-antigène, liaison récepteur-ligand
A sample plate for a thermal cycler suitable for performing a polymerase chain reaction (PCR) procedure includes a base plate and a number of reaction vessels extending upward from the base plate. The sample plate further includes a vertical wall surrounding an outer perimeter defined by the reaction vessels. The vertical wall can be a continuation vertical wall, an intermittent vertical wall, or a perforated vertical wall. The intermittent vertical wall can include a plurality of wall portions, each of which plurality of wall portions is separated from other wall portions via a plurality of gaps.
An instrument for detecting signal from a biological sample includes a pipettor module configured to hold a plurality of pipettes in respective pipette positions, to hold liquid in one or more pipette tips, and to pipette liquid in and out of the one or more pipette tips. Each of the one or more pipette tips has a pipette tip point. The instrument further includes one or more magnets positioned such that each of the one or more pipette tips is adjacent one of the one or more magnets.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/564 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour complexes immunologiques préexistants ou maladies auto-immunes
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
Methods and systems for sample target molecules are provided. In some embodiments, a method of detecting a target nucleic acid in multiple samples is provided.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
92.
ILLUMINATOR SYSTEMS HAVING LIGHT EMITTING DIODES WITH UV ACTIVATION
An imaging system including an illuminator apparatus or an epi-illumination apparatus that has LEDs for illuminations is provided for stain-free gel activation and fluorescent sample visualization. The illuminator apparatus includes a housing, a light source array disposed on at least one side surface of the housing and including at least one plurality of LEDs having each LED individually operable to output light of a predetermined color within a range of wavelengths, and a controller for controlling ranges of operational parameters of the at least one plurality of LEDs. The light emitted from the light source array incidents upon a sample having a gel that includes a product of UV light induced reaction between tryptophan and a haloalkane and the light emitted from the light source array includes ultraviolet (UV) light to excite a fluorescent response of the sample.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 21/00 - Recherche ou analyse des matériaux par l'utilisation de moyens optiques, c.-à-d. en utilisant des ondes submillimétriques, de la lumière infrarouge, visible ou ultraviolette
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 21/33 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique en utilisant la lumière ultraviolette
The present disclosure relates to the development of novel immunoassays for the detection of SARS-CoV-2 antigens and/or SARS-CoV-2 specific antibodies and, optionally, one or more cytokine in patient samples.
A61P 31/14 - Antiviraux pour le traitement des virus ARN
C07K 16/24 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des cytokines, des lymphokines ou des interférons
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/564 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour complexes immunologiques préexistants ou maladies auto-immunes
A reagent sleeve system such as can be used with various analyzers using chemical phenomena, analyzers and methods of using the reagent sleeve system and analyzer are described. The reagent sleeve system can include a reagent sleeve; and a plurality of removable reagent reservoirs located within the reagent sleeve, wherein each of the plurality of removable reagent reservoirs comprises a respective peripheral wall, and first and second end walls, whereby each of the plurality of removable reagent reservoirs contains a reagent located therein, and the first end wall is pierceable by a reagent extractor, and the reagent sleeve comprises a peripheral wall that encompasses the plurality of removable reagent reservoirs and holds the plurality of reagent reservoirs for sequential use.
Methods and systems for thermally controlling a chemical reaction in droplets. In an exemplary method, a first thermal zone and a second thermal zone having different temperatures from one another may be created in a reaction chamber. An emulsion including droplets encapsulated by a carrier fluid may be held in the reaction chamber. The droplets may have a density mismatch with the carrier fluid, and each droplet may include one or more reactants for the chemical reaction. An orientation of the reaction chamber may be changed to move the droplets from the first thermal zone to the second thermal zone, such that a rate of the chemical reaction changes in at least a subset of the droplets.
Provided herein is technology relating to the detection of analytes and particularly, but not exclusively, to methods, systems, compositions, and kits for detecting analytes such as nucleic acids, proteins, small molecules, metabolites, and other molecules using a technology based on the transient binding of detection probes in combination with a microfluidic device and/or a nanoparticle.
C12Q 1/6837 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide utilisant des réseaux de sondes ou des puces à sondes
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12Q 1/6811 - Méthodes de sélection pour la production ou l’élaboration d’oligonucléotides spécifiques cibles ou de molécules de liaison
C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
G01N 33/566 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant un support spécifique ou des protéines réceptrices comme réactifs pour la formation de liaisons par ligand
97.
METHOD OF PREPARING POLYMER-FILLED CHROMATOGRAPHY RESIN
This invention describes a method for preparation of stable hematology reference materials by producing synthetic hydrogel blood cell surrogates, which mimic human blood components in size, morphology, performance and functionality when analyzed using an automated hematology analyzer employing multiple detection technologies. Different hydrogel particles can be combined and mixed to prepare multi-parameter and multi-level hematology reference materials, which could be used for calibration, linearity verification, proficiency evaluation, and routine performance monitoring of modern automated hematology analyzers. These hydrogel particles can also be combined with processed and stabilized human blood components to prepare the reference materials of this invention.
G06F 17/30 - Recherche documentaire; Structures de bases de données à cet effet
G06F 3/0484 - Techniques d’interaction fondées sur les interfaces utilisateur graphiques [GUI] pour la commande de fonctions ou d’opérations spécifiques, p. ex. sélection ou transformation d’un objet, d’une image ou d’un élément de texte affiché, détermination d’une valeur de paramètre ou sélection d’une plage de valeurs
G06K 9/00 - Méthodes ou dispositions pour la lecture ou la reconnaissance de caractères imprimés ou écrits ou pour la reconnaissance de formes, p.ex. d'empreintes digitales
99.
AUTOMATED CHROMATOGRAM ANALYSIS FOR BLOOD TEST EVALUATION
A chromatogram analysis tool receives blood test data for a sample and divides the data into regions and determines a best-fit match template for each region. The chromatogram analysis tool determines a best-fit match for each region by comparing the blood test data to a set of templates associated with archetypical shapes of the region. The template with the highest r-squared value for the blood test data is the best-fit template. The chromatogram analysis tool generates a report based on the best-fit match templates for each region, which can indicate medical conditions or recommendations for additional testing.
An electronic component assembly having thermal pads with thermal vias coupling an image sensor and a camera board fab is provided for heat dissipation. The electronic component assembly can include: a circuit board having at least one thermal pad disposed on a top surface of the circuit board; and an image sensor disposed on the top surface of the circuit board, having at least one conductive pad disposed at at least one corner of the image sensor. The at least one thermal pad is coupled to the at least one conductive pad of the image sensor and the at least one thermal pad is formed with a plurality of first thermal vias penetrating the thermal pad and the circuit board for transfer of heat of the image sensor.
brevets
