A method of growing isolated mitochondria includes selecting a source of mitochondria and stem cells, extracting the stem cells and the mitochondria from the source and segregating the stem cells and the mitochondria into first and second pools respectively, incubating/transferring the extracted mitochondria into the extracted stem cells to produce packed stem cells, expanding the packed stem cells in a bioreactor, adjusting conditions of an environment of the bioreactor to favor growth of the mitochondria of the packed stem cells, converting the packed stem cells into megakaryocytes, isolating the mitochondria from the megakaryocytes, and applying a coating to the mitochondria following their isolation from the megakaryocytes.
Platelet-derived extracellular vesicles that include mitochondria (PEVs) are used in the treatment of an ocular disorder, or a symptom of the ocular disorder. The PEVs are collected by obtaining blood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mix, separating the mix into supernatant and platelet rich plasma (PRP), collecting the PRP, stimulating the collected PRP, thereby expelling extracellular vesicles from platelets in the PRP, and collecting the extracellular vesicles as PEVs.
A subject can be treated for cardiogenic shock and/or sepsis, or their symptoms using platelet-derived extracellular vesicles (PEVs) or mitlets that include PEVS. The PEVs include mitochondria. The PEVs can be collected by obtaining blood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mix, separating the mix into supernatant and platelet rich plasma (PRP), collecting the PRP and stimulating the collected PRP, thereby expelling extracellular vesicles from platelets in the PRP, and collecting the extracellular vesicles as the PEVs. PEVs can also be isolated from source cells grown in a bioreactor and suspended in a buffer to preserve the PEVs.
Energetics of chimeric antigen receptor (CAR)-containing cells can be enhanced by obtaining platelets from blood, obtaining mitlets from the platelets, and co-incubating the mitlets with the CAR-containing cells. Uptake of the mitlets into the CAR-containing cells yields enhanced CAR-containing cells having increased metabolic activity. The enhanced CAR-containing cells can be for treatment of an indication treatable by the CAR-containing cells. The mitlets can include coated mitochondria or vesicle-enclosed mitochondria.
A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
A method of growing isolated mitochondria includes selecting a source of mitochondria and stem cells, extracting the stem cells and the mitochondria from the source and segregating the stem cells and the mitochondria into first and second pools respectively, incubating/transferring the extracted mitochondria into the extracted stem cells to produce packed stem cells, expanding the packed stem cells in a bioreactor, adjusting conditions of an environment of the bioreactor to favor growth of the mitochondria of the packed stem cells, converting the packed stem cells into megakaryocytes, isolating the mitochondria from the megakaryocytes, and applying a coating to the mitochondria following their isolation from the megakaryocytes.
A subject can be treated for cardiogenic shock and/or sepsis, or their symptoms using platelet-derived extracellular vesicles (PEVs) or mitlets that include PEVS. The PEVs include mitochondria. The PEVs canbe collected by obtainingblood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mix, separating the mix into supernatant and platelet rich plasma (PRP), collecting the PRP and stimulating the collected PRP, thereby expelling extracellular vesicles from platelets in the PRP, and collecting the extracellular vesicles as the PEVs. PEVs can also be isolated from source cells grown in a bioreactor and suspended in a buffer to preserve the PEVs.
A subject can be treated for cardiogenic shock and/or sepsis, or their symptoms using platelet-derived extracellular vesicles (PEVs) or mitlets that include PEVS. The PEVs include mitochondria. The PEVs can be collected by obtaining blood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mix, separating the mix into supernatant and platelet rich plasma (PRP), collecting the PRP and stimulating the collected PRP, thereby expelling extracellular vesicles from platelets in the PRP, and collecting the extracellular vesicles as the PEVs. PEVs can also be isolated from source cells grown in a bioreactor and suspended in a buffer to preserve the PEVs.
Platelet-derived extracellular vesicles that include mitochondria (PEVs) are used in the treatment ofan ocular disorder, or a symptom ofthe ocular disorder. The PEVs are collected by obtaining blood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mix, separating the mix into supernatant and platelet rich plasma (PRP), collecting the PRP, stimulating the collected PRP, thereby expelling extracellular vesicles from platelets in the PRP, and collecting the extracellular vesicles as PEVs.
Platelet-derived extracellular vesicles that include mitochondria (PEVs) are used in the treatment of an ocular disorder, or a symptom of the ocular disorder. The PEVs are collected by obtaining blood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mix, separating the mix into supernatant and platelet rich plasma (PRP), collecting the PRP, stimulating the collected PRP, thereby expelling extracellular vesicles from platelets in the PRP, and collecting the extracellular vesicles as PEVs.
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