The subject disclosure presents, in some embodiments, is systems, devices and methods for multi-sample analyte analysis. In some such embodiments, a method is presented which includes labeling at least one target analyte in each of a plurality of individual samples with at least one unique label probe. The method further includes combining the plurality of individual samples into a mixture, and flowing the mixture within at least one fluidic channel/lane having arranged thereon or in fluid communication with at least one set of a plurality of spatially distinct capture zones. Embodiments of the present disclosure additionally relate to, among other things, systems, devices, and related methods for operating the same, to analyze and rapidly identify and quantify microbial pathogens in environmental, food, and clinical settings. Specifically, a rapid one-step FISH method where fixation, permeabilization and addition of fluorescent labeled probe all occur in one step on a ferrofluidic system.
Methods and systems for determining a microbial risk assessment for an entity are provided. Microbial data and non-microbial data are received and identified as corresponding to the entity and one or more biological processes associated with the entity. The microbial data and non-microbial data are mapped to one or more risk vectors impacting a state of the one or more biological processes. One or more risk vector ratings are determined for the one or more risk vectors based on the microbial and non-microbial data. A microbial risk assessment including a microbial risk rating for the entity is determined based on the one or more risk vector ratings, where the microbial risk assessment is indicative of a risk of the one or more biological processes to the entity. An expected loss value for the one or more biological processes associated with the entity is determined based on the microbial risk assessment.
Embodiments of the present disclosure are directed to ferrofluid-based assay methods, systems and device for detecting one or more parasite oocyst or egg, and more specifically, to detecting parasite oocysts or eggs in fecal matter of livestock, so as to determine parasitic infections within such livestock. Such embodiments are configured to help in the ability to maintain healthy livestock for human consumption.
in situDD-amino acid analogs, such as fluorescent D-amino acids or D-amino acids with a biorthogonal tag to enable chemical conjugation of a fluorophore (e.g. by click chemistry). The disclosure further provides methods for quantifying the TVB by cell manipulation using ferrofluid within a microfluidic device.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
5.
SYSTEMS, DEVICES AND METHODS FOR DETERMING MOST PROBABLE NUMBER IN BIOLOGICAL SAMPLE ANALYSIS
Embodiments of the present disclosure include systems, devices and methods for increasing the accuracy of an MPN, using assay instrumentation. For example, such embodiments can be accomplished by pre-loading the assays system with standard curves generated from measurements made with dilutions of known levels of pathogens. When such an approach is used, for each sample, the value from the last positive dilution and a calibrated assay count can both be used to provide a more accurate CFU per sample value than would be determined from just the last positive dilution alone.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Assays for research purposes. Diagnostic assays and reagents for detecting microbial presence in fluid and biological samples and assessing microbial risk; diagnostic assays and reagents for identifying pathogenic and non-pathogenic microbes present in livestock. Scientific apparatus and software for detecting microbial presence in fluid and biological samples and assessing microbial risk; Scientific apparatus and software for diagnosing and monitoring particles, pathogens and toxins for purposes of laboratory research, food safety research, and agricultural research; Scientific apparatus and operating software for cell manipulation and sorting for purposes of laboratory research in the fields of food safety and agriculture; Scientific apparatus and software for cell transport for purposes of laboratory research in the fields of food safety and agriculture, namely, cell culture chambers and computer software for tracking biological samples for purposes of transport; downloadable software for use in collecting, tracking and analyzing data relating to microbes, bio-markers, morphology and molecular signals; downloadable software for microbial risk assessment and data analytics in the fields of food safety and agriculture, namely, software for collection and assessment of quantitative microbial data on pathogenic and non-pathogenic microorganisms; downloadable software for data collection and assessment relating to intervention management in the fields of food safety and agriculture. Agricultural safety testing and consultation related thereto; livestock safety testing and consultation related thereto; food safety testing and consultation related thereto; Scientific research, namely, scientific data analysis and risk assessment relating to pathogens, toxins and contaminants; Providing temporary use of non-downloadable platform and software for use in collecting, tracking and analyzing data relating to microbes, bio-markers, morphology and molecular signals; Providing temporary use of non-downloadable platform and software for microbial risk assessment and data analytics in the fields of food safety and agriculture, namely, software for collection and assessment of quantitative microbial data on pathogenic and non-pathogenic microorganisms; Providing temporary use of non-downloadable software for data collection and assessment relating to intervention management in the fields of food safety and agriculture; Custom design and development of chemical reagents and biochemical assays; Data analysis for the purpose of intervention management in the fields of food safety, agriculture, and livestock production.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
assays for research purposes diagnostic assays and reagents for detecting microbial presence in fluid and biological samples and assessing microbial risk for medical or veterinary use; diagnostic assays and reagents for identifying pathogenic and non-pathogenic microbes present in livestock for medical or veterinary use scientific apparatus and downloadable software for detecting microbial presence in fluid and biological samples and assessing microbial risk; Scientific apparatus and downloadable software for diagnosing and monitoring particles, pathogens and toxins for purposes of laboratory research, food safety research, and agricultural research; Scientific apparatus and downloadable operating software for cell manipulation and sorting for purposes of laboratory research in the fields of food safety and agriculture; Scientific apparatus and downloadable software for cell transport for purposes of laboratory research in the fields of food safety and agriculture, namely, cell culture chambers and downloadable computer software for tracking biological samples for purposes of transport; downloadable software for use in collecting, tracking and analyzing data relating to microbes, bio-markers, morphology and molecular signals; downloadable software for microbial risk assessment and data analytics in the fields of food safety and agriculture, namely, downloadable software for collection and assessment of quantitative microbial data on pathogenic and non-pathogenic microorganisms; downloadable software for data collection and assessment relating to intervention management in the fields of food safety and agriculture Agricultural product safety testing and consultation related thereto; livestock safety testing and consultation related thereto; food safety testing and consultation related thereto; Scientific research, namely, scientific data analysis and risk assessment relating to pathogens, toxins and contaminants; Providing temporary use of non-downloadable computer software platforms and software for use in collecting, tracking and analyzing data relating to microbes, bio-markers, morphology and molecular signals; Providing temporary use of non-downloadable computer software platforms and software for microbial risk assessment and data analytics in the fields of food safety and agriculture, namely, software for collection and assessment of quantitative microbial data on pathogenic and non-pathogenic microorganisms; Providing temporary use of non-downloadable software for data collection and assessment relating to intervention management in the fields of food safety and agriculture; Custom design and development of chemical reagents and biochemical assays; Data analysis for the purpose of intervention management in the fields of food safety, agriculture, and livestock production
8.
Background defocusing and clearing in ferrofluid-based capture assays
Devices, methods, and systems are provided for extracting particles from a ferrofluid. Such methods may comprise receiving a flow of ferrofluid comprising target particles and background particles and generating a first, focusing magnetic field to focus the target particles towards a capture region. The capture region may capture the target particles and a plurality of background particles. A second, defocusing magnetic field may be configured to remove background particles from the capture region. A detector may be used to detect the target particles bound to the target region.
B03C 1/253 - Magnetic separation acting directly on the substance being separated with material carried by oscillating fieldsMagnetic separation acting directly on the substance being separated with material carried by travelling fields, e.g. generated by stationary magnetic coilsEddy-current separators, e.g. sliding ramp with material carried by travelling fields obtained by a linear motor
B03C 1/023 - Separation using Lorentz force, i.e. deflection of electrically charged particles in a magnetic field
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
9.
SYSTEMS AND METHODS FOR BEAD-BASED ASSAYS IN FERROFLUIDS
Some embodiments of the present disclosure are directed to systems and methods for separating, directing, and/or extracting a target molecule from a mix of molecules and may comprise a plurality of non-magnetic beads suspended in a ferro fluid, where the non-magnetic beads may be functionalized with at least one predetermined first molecule configured to bind with a target particle. A microfluidic device may be included which may comprise at least one microfluidic channel, the device configured to dynamically and/or statically receive an amount of the mix. Magnetic field means may be included and may be configured to apply a magnetic field to at least a portion of the at least one channel to exert an indirect force on the non-magnetic heads in the ferro fluid mix, and separate the non-magnetic beads from the ferrofluid. The beads may then be directed to at least one receptor region. At least one outlet may be provided which is arranged to be in communication with the at least one microfluidic channel, the at least one outlet may be configured to receive and extract the separated non-magnetic beads from the ferrofluid.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
10.
Systems, devices and methods for cartridge securement
In certain embodiments, the disclosure provides an inflatable bladder lid that configures with a cartridge configured for assay testing. The inflatable bladder provides substantially uniform pressure to the cartridge. The pressure is substantially distributed across the one or more regions of the cartridge to extend pressure over a wide cartridge surface. At least a portion of the bladder lid may comprise a flexible membrane material that inflates and stretches over at least a portion of the cartridge to conformally contact its first/top surface.
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
B07B 1/46 - Constructional details of screens in generalCleaning or heating of screens
Devices, methods, and systems are provided for extracting particles from a ferrofluid and for rapid affinity measurements. Such systems may comprise a fluidic channel or chamber configured to include a ferrofluid having a plurality of target particles and background particles. The systems may include a capture region configured to capture at least a portion of the plurality of target particles. In addition, the systems include a first magnetic field generator and a second magnetic field generator. The first magnetic field generator may be arranged proximate to the fluidic channel, the first magnetic field generator being configured to generate a first magnetic field configured to direct the plurality of target particles towards the capture region. The second magnetic field generator can be arranged to be proximate to the capture region, and is further configured to generate an affinity thresholding magnetic field configured to remove background particles from the capture region.
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
12.
Systems and methods for active particle separation
A device and method for extracting particles contained in a ferrofluid medium are provided. Such methods may comprise suspending particles of different sizes in a ferrofluid medium and containing the ferrofluid medium in a cylindrical reservoir, and applying a first magnetic field to at least a portion of the reservoir. The first magnetic field is configured to indirectly exert a force on at least a portion of the particles of a predetermined size, and direct the portion of particles in a desired direction.
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
C02F 1/44 - Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
C02F 1/48 - Treatment of water, waste water, or sewage with magnetic or electric fields
B03C 1/023 - Separation using Lorentz force, i.e. deflection of electrically charged particles in a magnetic field
B03C 1/033 - Component partsAuxiliary operations characterised by the magnetic circuit
B03C 1/253 - Magnetic separation acting directly on the substance being separated with material carried by oscillating fieldsMagnetic separation acting directly on the substance being separated with material carried by travelling fields, e.g. generated by stationary magnetic coilsEddy-current separators, e.g. sliding ramp with material carried by travelling fields obtained by a linear motor
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
13.
SYSTEMS AND METHODS FOR THREE-DIMENSIONAL EXTRACTION OF TARGET PARTICLES FERROFLUIDS
Systems, methods and devices are presented for extracting target particles within a ferrofluid medium. In some embodiments, a fluidic channel receives a flow of a mix of one or more types of target particles, where at least one magnetic field source is configured to react with the flow such that a force (indirect or direct) is placed on the particles of the mix, across the width and/or the height of the fluidic channel. An extraction opening placed on one wall is provided and configured to extract at least one type of target particle.
Devices, methods, and systems are provided for extracting particles from a ferrofluid and for rapid affinity measurements. Such systems may comprise a fluidic channel or chamber configured to include a ferrofluid having a plurality of target particles and background particles. The systems may include a capture region configured to capture at least a portion of the plurality of target particles. In addition, the systems include a first magnetic field generator and a second magnetic field generator. The first magnetic field generator may be arranged proximate to the fluidic channel, the first magnetic field generator being configured to generate a first magnetic field configured to direct the plurality of target particles towards the capture region. The second magnetic field generator can be arranged to be proximate to the capture region, and is further configured to generate an affinity thresholding magnetic field configured to remove background particles from the capture region.
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
B03C 1/023 - Separation using Lorentz force, i.e. deflection of electrically charged particles in a magnetic field
B03C 1/033 - Component partsAuxiliary operations characterised by the magnetic circuit
In certain embodiments, the disclosure provides an inflatable bladder lid that configures with a cartridge configured for assay testing. The inflatable bladder provides substantially uniform pressure to the cartridge. The pressure is substantially distributed across the one or more regions of the cartridge to extend pressure over a wide cartridge surface. At least a portion of the bladder lid may comprise a flexible membrane material that inflates and stretches over at least a portion of the cartridge to conformally contact its first/top surface.
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
B07B 1/46 - Constructional details of screens in generalCleaning or heating of screens
Some embodiments of the present disclosure are directed to systems and methods for separating, directing, and/or extracting a target molecule from a mix of molecules and may comprise a plurality of non-magnetic beads suspended in a ferro fluid, where the non-magnetic beads may be functionalized with at least one predetermined first molecule configured to bind with a target particle. A microfluidic device may be included which may comprise at least one microfluidic channel, the device configured to dynamically and/or statically receive an amount of the mix. Magnetic field means may be included and may be configured to apply a magnetic field to at least a portion of the at least one channel to exert an indirect force on the non-magnetic heads in the ferro fluid mix, and separate the non-magnetic beads from the ferrofluid. The beads may then be directed to at least one receptor region. At least one outlet may be provided which is arranged to be in communication with the at least one microfluidic channel, the at least one outlet may be configured to receive and extract the separated non-magnetic beads from the ferrofluid.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
Devices, methods, and systems are provided for extracting particles from a ferrofluid and for rapid affinity measurements. Such systems may comprise a fluidic channel or chamber configured to include a ferrofluid having a plurality of target particles and background particles. The systems may include a capture region configured to capture at least a portion of the plurality of target particles. In addition, the systems include a first magnetic field generator and a second magnetic field generator. The first magnetic field generator may be arranged proximate to the fluidic channel, the first magnetic field generator being configured to generate a first magnetic field configured to direct the plurality of target particles towards the capture region. The second magnetic field generator can be arranged to be proximate to the capture region, and is further configured to generate an affinity thresholding magnetic field configured to remove background particles from the capture region.
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
Devices, methods, and systems are provided for extracting particles from a ferrofluid. Such methods may comprise receiving a flow of ferrofluid comprising target particles and background particles and generating a first, focusing magnetic field to focus the target particles towards a capture region. The capture region may capture the target particles and a plurality of background particles. A second, defocusing magnetic field may be configured to remove background particles from the capture region. A detector may be used to detect the target particles bound to the target region.
A system for determining drug effectiveness on a plurality of cells is described. The system includes flowing a ferrofluid mixed with a plurality of biological cells through an inlet portion of a cartridge, the cartridge comprising a plurality of microfluidic channels, the inlet is in communication with a portion of each of the plurality of channels, applying a magnetic field proximate at least one of the inlet portion and the plurality of micro-channels, wherein the magnetic field is configured to apply an indirect force on the mix, separating biologic cells according to at least a first type as the mix flows in a first direction; and directing at least the first type of cells toward a first sensor functionalized with receptors via at least one of the micro-channels, the sensor arranged proximate to a second portion of at least one of the micro-channels downstream from the first inlet portion.
A device for determining the identity and concentration of target particles within a biocompatible ferrofluid medium is described. The system includes a fluidic channel that a sample of particles flow through; at least one magnetic field source configured to react repulsively with the particles; a channel wall with at least one receptor regions placed serially along the flow direction; and at least one thin electrode placed between the receptor regions to track changes in local impedance every time a target particle passes through the fluidic channel in close proximity.
Systems, methods and devices are presented for extracting target particles within a ferrofluid medium. In some embodiments, a fluidic channel receives a flow of a mix of one or more types of target particles, where at least one magnetic field source is configured to react with the flow such that a force (indirect or direct) is placed on the particles of the mix, across the width and/or the height of the fluidic channel. An extraction opening placed on one wall is provided and configured to extract at least one type of target particle.
B01D 35/06 - Filters making use of electricity or magnetism
B01D 59/48 - Separation by mass spectrography using electrostatic and magnetic fields
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
22.
SYSTEMS AND METHODS FOR ACTIVE PARTICLE SEPARATION
A device and method for extracting particles contained in a ferrofluid medium are provided. Such methods may comprise suspending particles of different sizes in a ferrofluid medium and containing the ferrofluid medium in a cylindrical reservoir, and applying a first magnetic field to at least a portion of the reservoir. The first magnetic field is configured to indirectly exert a force on at least a portion of the particles of a predetermined size, and direct the portion of particles in a desired direction.
Some embodiments of the present disclosure are directed to systems and methods for separating, directing, and/or extracting a target molecule from a mix of molecules and may comprise a plurality of non-magnetic beads suspended in a ferro fluid, where the non-magnetic beads may be functionalized with at least one predetermined first molecule configured to bind with a target particle. A microfluidic device may be included which may comprise at least one microfluidic channel, the device configured to dynamically and/or statically receive an amount of the mix. Magnetic field means may be included and may be configured to apply a magnetic field to at least a portion of the at least one channel to exert an indirect force on the non-magnetic beads in the ferro fluid mix, and separate the non-magnetic beads from the ferrofluid. The beads may then be directed to at least one receptor region. At least one outlet may be provided which is arranged to be in communication with the at least one microfluidic channel, the at least one outlet may be configured to receive and extract the separated non-magnetic beads from the ferrofluid.
B03C 1/32 - Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
G01N 33/49 - Physical analysis of biological material of liquid biological material blood
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
