The purpose of the present invention is to provide a highly versatile production method with which it is possible to use any hydrophobic peptide as the object of purification regardless of the type of amino acid residue at the N-terminal and to flexibly select a hydrophilic unit in accordance with the type of hydrophobic peptide when conducting HPLC purification by hydrophilizing a solid-phase-synthesized hydrophobic peptide using a hydrophilic unit. The present invention is a method for obtaining a purified peptide from a supported crude peptide configured from a support and a first peptide chain bonded on the C-terminal side to this support, wherein the method is characterized by having: a hydrophilization step A for obtaining a support-free hydrophilized peptide by introducing a linker and a hydrophilic unit in the stated order stepwise or in a single step to an amino group of a supported crude peptide; a support-cutting step B for cleaving the bond between the first peptide chain and the support at any step from before bonding the linker to the supported crude peptide until obtaining the hydrophilized peptide, or after having obtained a supported hydrophilized peptide; a chromatographic purification step for dissolving the support-free hydrophilized peptide obtained through the hydrophilization step A and the support-cutting step B in water, and treating the dissolved support-free hydrophilized peptide by liquid chromatography; and a linker-cutting step for cleaving the bond between the linker and the first peptide chain contained in the chromatographically purified support-free hydrophilized peptide by chemical treatment.
The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
C07D 209/02 - Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
The present invention is generally directed to biological assays. More specifically it is directed to optimal FRET pairs and methods related to their use. In a composition aspect, the present invention is directed to FRET-based protease substrates selected from the list of substrates shown in the Detailed Description. In a method aspect, the present invention is directed to a method of performing a FRET-based assay, where the assay includes the following steps: adding a solution or suspension containing one or more different proteins to a reaction vessel; adding a liquid comprising a FRET-substrate to the reaction vessel, wherein the FRET substrate is selected from the list of substrates shown in the Detailed Description. In a kit aspect, the present invention is directed to a kit for performing a FRET-based assay, wherein the kit comprises a protease substrate. The protease substrate is selected from the list of substrates shown in the Detailed Description.
C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
Methods for performing FRET-based biological assays using optimized FRET pairs, said methods comprise adding a solution or suspension containing one or more different proteins to a reaction vessel, adding a liquid comprising a FRET-based protease substrate to the reaction vessel Kit for performing a FRET-based assay, wherein the kit comprises a protease substrate.
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C07K 2/00 - Peptides of undefined number of amino acidsDerivatives thereof
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C07D 225/00 - Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom
C07D 245/00 - Heterocyclic compounds containing rings of more than seven members having two nitrogen atoms as the only ring hetero atoms
C07D 209/02 - Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and the 3-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivate compounds having one or more benzo nitrogens.
C07D 209/02 - Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
01 - Chemical and biological materials for industrial, scientific and agricultural use
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals; namely, peptide and immunochemistry reagents used
in the bio-technology and pharmaceutical industries. Manufacture of peptides and antibodies to the specification
of others for academic and industrial research. Chemical and bio-chemical analysis, chemical laboratories
and chemical research.
01 - Chemical and biological materials for industrial, scientific and agricultural use
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biomedical compounds, namely, monoclonal antibodies used in analyzing and detecting certain enzymes, receptors, oligonucleotides and proteins for in vitro scientific laboratory or research use in the biotechnology and pharmaceutical industries; biomedical compounds in the nature of amino acids, namely, peptides used in analyzing and detecting certain enzymes, receptors, oligonucleotides and proteins for laboratory or research use in the biotechnology and pharmaceutical industries; chemical reagents for non-medical purposes in the biotechnology and pharmaceutical industries Manufacture of peptides and antibodies, namely, synthetic peptides for pharmaceutical purposes and monoclonal antibodies for in vitro scientific or research use, to the specification of others for academic and industrial research Chemical and bio-chemical analysis, chemical laboratories and chemical research
15.
Cyanine dyes and their applications as luminescence quenching compounds
The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.
C07D 209/46 - Iso-indolesHydrogenated iso-indoles with an oxygen atom in position 1
C07D 209/02 - Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
