Goods & Services

Diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection Point-of-care medical device, namely, automated molecular diagnostic testing device; medical diagnostic apparatus, namely, molecular diagnostic tests Polymerase chain reaction (pcr) testing for medical purposes; molecular diagnostic testing, monitoring and reporting services to aid in the diagnosis and resolution of diseases

Goods & Services

Providing access to medical data and information via the internet Providing temporary use of non-downloadable software for use in polymerase chain reaction (pcr) testing for medical and scientific research purposes; providing temporary use of non-downloadable cloud-based computer software for clinical diagnostics; providing temporary use of non-downloadable cloud-based software for delivering laboratory results; providing medical testing services and information in the field of disease detection; providing temporary use of non-downloadable cloud-based secure database software that allows patients to access their laboratory results and share their results with medical and healthcare providers; scientific and medical research in the field of disease detection; platform as a service (paas) featuring cloud-based digital healthcare computer software platform that allows users to manage their health and healthcare; providing temporary use of non-downloadable software for use for use with medical testing apparatus and instruments, namely, for use in data analysis; providing temporary use of non-downloadable software for remote monitoring, maintenance, and troubleshooting of medical testing apparatus and instruments; providing temporary use of non-downloadable software for reviewing the status of medical testing apparatus and instruments Molecular diagnostic reporting services to aid in the diagnosis and resolution of diseases; medical diagnostic reporting services; medical testing, screening, and assessment services using a cloud-based portal

Goods & Services

Diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection Providing access to medical data and information via the internet Point-of-care medical device, namely, automated molecular diagnostic testing device; medical diagnostic apparatus, namely, molecular diagnostic tests Providing temporary use of non-downloadable software for use in polymerase chain reaction (pcr) testing for medical and scientific research purposes; providing temporary use of non-downloadable cloud-based computer software for clinical diagnostics; providing temporary use of non-downloadable cloud-based software for delivering laboratory results; providing medical testing services and information in the field of disease detection; providing temporary use of non-downloadable cloud-based secure database software that allows patients to access their laboratory results and share their results with medical and healthcare providers; scientific and medical research in the field of disease detection; platform as a service (paas) featuring cloud-based digital healthcare computer software platform that allows users to manage their health and healthcare; providing temporary use of non-downloadable software for use for use with medical testing apparatus and instruments, namely, for use in data analysis; providing temporary use of non-downloadable software for remote monitoring, maintenance, and troubleshooting of medical testing apparatus and instruments; providing temporary use of non-downloadable software for reviewing the status of medical testing apparatus and instruments Polymerase chain reaction (pcr) testing for medical purposes; molecular diagnostic testing, monitoring and reporting services to aid in the diagnosis and resolution of diseases; medical diagnostic testing, monitoring, and reporting services; medical testing, screening, and assessment services using a cloud-based portal

Abstract

Provided herein are methods for preparing a sample comprising a nucleic acid. In some embodiments, the method comprises heating the sample above its boiling point to rapidly extract nucleic acid from biological entities present in the sample.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Disclosed herein are methods, compositions, and devices for the detection of neutralizing antibodies for a pathogen of interest. In some embodiments, a covalent coupling between a viral protein and a receptor protein is utilized to reduce dissociation of the viral protein/receptor complex.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present invention describes a method and device enabling sample processing, fast polymerase chain reaction amplification, and real-time reading of a large number of targets simultaneously while using a volume of reaction compatible with molecular assays. The present invention can be part of a cartridge allowing sample to answer nucleic acid analytic system (NAAT) or can be used as a stand-alone device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Provided herein are methods for preparing a sample comprising a nucleic acid. In some embodiments, the method comprises heating the sample above its boiling point to rapidly extract nucleic acid from biological entities present in the sample.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed herein are methods, compositions, and devices for the detection of neutralizing antibodies for a pathogen of interest. In some embodiments, a covalent coupling between a viral protein and a receptor protein is utilized to reduce dissociation of the viral protein/receptor complex.

IPC Classes  ?

• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• C07K 16/08 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses

Goods & Services

Clinical diagnostic preparations for clinical use; diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection; medical diagnostic reagents and assays for testing of body fluids. Downloadable computer software; Downloadable computer software for clinical diagnostics; downloadable software for delivering laboratory results; downloadable software that allows patients to access their laboratory results and share their results with medical and healthcare providers. Medical apparatus and instruments; Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing; medical apparatus and instrument for running assay disks for COVID-19 diagnostic tests; medical diagnostic apparatus for testing for diseases; diagnostic apparatus for medical use, namely, a diagnostics medical apparatus for disease detection; medical apparatus and instruments, namely, automated assay systems for medical diagnostic purposes; portable diagnostic medical testing instrument for use in point-of-case diagnostics for use in disease detection. Providing temporary use of non-downloadable software; software as a service [SaaS]; providing non-downloadable cloud-based computer software for clinical diagnostics; providing temporary use of nondownloadable cloud-based software for delivering laboratory results; providing temporary use of nondownloadable cloud-based secure database software that allows patients to access their laboratory results and share their results with medical and healthcare providers; scientific and medical research in the field of disease detection; providing a cloud-based digital healthcare platform that allows users to manage their health and healthcare. Medical services; Medical testing, monitoring, and reporting services; medical clinical diagnostic testing, monitoring, and reporting services; medical testing for diagnostic or treatment purposes; medical testing, screening, and assessment services using a cloud-based portal; healthcare management services in the field of disease detection; providing medical testing services and information in the field of disease detection.

Goods & Services

Clinical diagnostic preparations for clinical use; diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection; medical diagnostic reagents and assays for testing of body fluids. Downloadable computer software; Downloadable computer software for clinical diagnostics. Medical apparatus and instruments; Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing; medical apparatus and instrument for running assay disks for COVID-19 diagnostic tests; medical diagnostic apparatus for testing for diseases; medical apparatus and instruments, namely, automated assay systems for medical diagnostic purposes; portable diagnostic medical testing instrument for use in point-of-case diagnostics for use in disease detection. Providing temporary use of non-downloadable software; software as a service [SaaS]; Providing nondownloadable cloud-based computer software for clinical diagnostics; providing temporary use of nondownloadable cloud-based software for delivering laboratory results; providing use of downloadable software for delivering laboratory results.

Goods & Services

(1) Clinical diagnostic reagents; diagnostic preparations for clinical laboratory use; diagnostic preparations for medical laboratory use; diagnostic reagents for clinical laboratory use; diagnostic reagents for medical diagnostic use; diagnostic reagents for medical laboratory use; diagnostic reagents for medical purposes; medical diagnostic reagents; medical diagnostic reagents and assays for testing of body fluids (2) Clinical diagnostic preparations for clinical use; portable diagnostic medical testing instrument for use in point-of-case diagnostics for use in disease detection; diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection; medical diagnostic reagents and assays for testing of body fluids (3) Downloadable computer software for clinical diagnostics; providing use of downloadable software for delivering laboratory results; providing use of downloadable software that allows patients to access their laboratory results and share their results with medical and healthcare providers (4) Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing; medical apparatus and instrument for running assay disks for COVID-19 diagnostic tests; medical diagnostic apparatus for testing for diseases; diagnostic apparatus for medical use, namely, a diagnostics platform for disease detection; medical apparatus and instruments, namely, automated assay systems for medical diagnostic purposes (1) Nondownloadable cloud-based computer software for clinical diagnostics; providing temporary use of nondownloadable cloud-based software for delivering laboratory results; providing medical testing services and information in the field of disease detection; providing temporary use of nondownloadable cloud-based secure database software that allows patients to access their laboratory results and share their results with medical and healthcare providers; scientific and medical research in the field of disease detection; providing a cloud-based digital healthcare platform that allows users to manage their health and healthcare (2) Medical diagnostic services; medical diagnostic testing, monitoring and reporting services (3) Medical testing, monitoring, and reporting services; medical clinical diagnostic testing, monitoring, and reporting services; medical testing for diagnostic or treatment purposes; medical testing, screening, and assessment services using a cloud-based portal; healthcare management services in the field of disease detection

Goods & Services

(1) Clinical diagnostic reagents; diagnostic preparations for clinical laboratory use; diagnostic preparations for medical laboratory use; diagnostic reagents for clinical laboratory use; diagnostic reagents for medical diagnostic use; diagnostic reagents for medical laboratory use; diagnostic reagents for medical purposes; medical diagnostic reagents; medical diagnostic reagents and assays for testing of body fluids (2) Clinical diagnostic preparations for clinical use; portable diagnostic medical testing instrument for use in point-of-case diagnostics for use in disease detection; diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection; medical diagnostic reagents and assays for testing of body fluids (3) Downloadable computer software for clinical diagnostics; providing use of downloadable software for delivering laboratory results (4) Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing; medical apparatus and instrument for running assay disks for COVID-19 diagnostic tests; medical diagnostic apparatus for testing for diseases; medical apparatus and instruments, namely, automated assay systems for medical diagnostic purposes (1) Nondownloadable cloud-based computer software for clinical diagnostics; providing temporary use of nondownloadable cloud-based software for delivering laboratory results (2) Medical diagnostic services; medical diagnostic testing, monitoring and reporting services (3) Medical testing, monitoring, and reporting services; medical clinical diagnostic testing, monitoring, and reporting services; medical testing for diagnostic or treatment purposes; medical testing, screening, and assessment services using a cloud-based portal

Goods & Services

Diagnostic preparations for clinical laboratory use diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection, namely, respiratory and gastrointestinal infectious diseases, women's health, sexually transmitted diseases, lyme disease, sepsis, bacteremia, antibiotic resistance, hospital acquired diseases; medical diagnostic reagents and assays for testing of body fluids Downloadable computer software for clinical diagnostics; downloadable software for delivering laboratory results Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing in the fields of infectious diseases, cancer detection, tissue-based diagnostics testing and molecular diagnostics; medical apparatus and instrument for running assay disks for COVID-19 diagnostic tests; medical diagnostic apparatus for testing for diseases; medical apparatus and instruments, namely, automated assay systems for medical diagnostic purposes; portable diagnostic medical testing instrument for use in point-of-case diagnostics for use in disease detection Providing temporary use of non-downloadable cloud-based computer software for clinical diagnostics; providing temporary use of nondownloadable cloud-based software for delivering laboratory results Medical diagnostic testing, monitoring, and reporting services; medical clinical diagnostic testing, monitoring, and reporting services; medical diagnostic testing for diagnostic or treatment purposes; medical testing, screening, and assessment services using a cloud-based portal

Goods & Services

Diagnostic preparations for clinical laboratory use diagnostic kits comprised of medical diagnostic reagents and assays for testing of bodily fluids for use in disease detection, namely, respiratory and gastrointestinal infectious diseases, women's health, sexually transmitted diseases, lyme disease, sepsis, bacteremia, antibiotic resistance, hospital acquired diseases; medical diagnostic reagents and assays for testing of body fluids Downloadable computer software for clinical diagnostics; downloadable software for delivering laboratory results; downloadable software that allows patients to access their laboratory results and share their results with medical and healthcare providers Medical apparatus and instrument for diagnostic use, namely, apparatus for medical diagnostic testing in the fields of infectious diseases, cancer detection, tissue-based diagnostics testing and molecular diagnostics; medical apparatus and instrument for running assay disks for COVID-19 diagnostic tests; medical diagnostic apparatus for testing for diseases; diagnostic apparatus for medical use, namely, a diagnostics platform for disease detection; medical apparatus and instruments, namely, automated assay systems for medical diagnostic purposes; portable diagnostic medical testing instrument for use in point-of-case diagnostics for use in disease detection Providing temporary use of non-downloadable cloud-based computer software for clinical diagnostics; providing temporary use of nondownloadable cloud-based software for delivering laboratory results; providing medical testing services and information in the field of disease detection; providing temporary use of nondownloadable cloud-based secure database software that allows patients to access their laboratory results and share their results with medical and healthcare providers; scientific and medical research in the field of disease detection; platform as a service (PAAS) featuring cloud-based digital healthcare computer softare platform that allows users to manage their health and healthcare Medical diagnostic testing, monitoring, and reporting services; medical clinical diagnostic testing, monitoring, and reporting services; medical diagnostic testing for diagnostic or treatment purposes; medical testing, screening, and assessment services using a cloud-based portal; healthcare management services in the field of disease detection, namely, disease management programs

Abstract

A field portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.

IPC Classes  ?

• G01N 19/02 - Measuring coefficient of friction between materials
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 29/02 - Analysing fluids
• G01N 29/036 - Analysing fluids by measuring frequency or resonance of acoustic waves
• H03H 9/02 - Networks comprising electromechanical or electro-acoustic elementsElectromechanical resonators Details
• H03H 9/145 - Driving means, e.g. electrodes, coils for networks using surface acoustic waves

Abstract

A field portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

An automated system communicated to a remote server for diagnostically field testing a sample taken from a patient using an automated portable handheld instrument to determine the presence of Covid-19 and/or antibodies thereto includes microfluidic circuits defined in a rotatable disk for performing a bioassay using a microarray to generate an electrical signal indicative of a bioassay measurement; the microarray operationally positioned in the microfluidic circuit; one or more lasers; one or more positionable valves in the microfluidic circuit; and a backbone unit for rotating the disk according to a protocol to perform the bioassay, for controlling the lasers to selectively open the positionable valves in the microfluidic disk, for operating the microarray to generate a digital image as a bioassay measurement; for communicating the bioassay measurement to the remote server, and for associating the performed bioassay and its corresponding bioassay measurement to the patient.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G16B 50/30 - Data warehousingComputing architectures

Abstract

The disclosure relates to antibody conjugates or aptamer conjugates comprising an antibody linked to a lipid vesicle comprising a detectable label, methods for detecting a marker or several markers in a sample by using said antibody and aptamer conjugates. The present disclosure further relates to microfluidics devices to detect one or more markers in a sample by using said antibody and aptamer conjugates.

IPC Classes  ?

• G01N 33/532 - Production of labelled immunochemicals
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

The disclosure relates to antibody conjugates comprising an antibody linked to a polynucleotide, aptamer conjugates comprising an aptamer linked to a polynucleotide, and methods for detecting a marker or several markers in a sample by using said antibody and aptamer conjugates. The present disclosure also relates to microfluidics devices for detecting markers in a sample. The present disclosure further relates to methods for detecting a microorganism or several microorganisms in a sample and a microfluidics device to be used to detect such microorganisms.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

A field effect transistor comprising a chelator or derivatized chelator, a device for detecting a marker in a sample comprising said sensor, a method for detecting metal ions in a sample comprising said sensor or device and a sensor and method for detecting a marker in a sample.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 27/414 - Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS

Abstract

The invention includes a method of assaying an analyte in a sample in a portable, handheld microfluidic reader. The method includes the steps of: inserting the sample in the reader; capturing the analyte with a first antibody having a DNA tag attached thereto; capturing the analyte in the sample with a second antibody attached to a surface or having a magnetic nanoparticle (MNP) attached thereto; where a sandwich including the magnetic nanoparticle, first and second antibodies, the analyte and the DNA tag is formed; replicating the DNA tag using isothermal amplification to a predetermined amount of DNA tags detectable by a detector sufficient to overcome the minimal mass sensitivity limitations of the detector; and measuring the amount of replicated DNA tags using the detector. The invention also includes an apparatus or handheld portable field microfluidic reader in which the method is performed.

IPC Classes  ?

• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/541 - Double or second antibody
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

The illustrated embodiments of the invention include an automated method of assaying a viral and antibody analyte in a sample in a portable, handheld microfluidic reader having a SAW detector with a minimal mass sensitivity limitation. The automated method includes the steps of automatically performing the assay with the SAW detector with enhanced sensitivity as in Optikus I, but also includes the steps of automatically disposing a second portion of the sample on a microarray, selectively automatically probing the second portion of the sample for antibodies corresponding to the at least one selected virus using the microarray, and automatically reading the microarray using a fluorescent camera to identify antibodies in the second portion of the sample.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection
• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
• G01N 21/64 - FluorescencePhosphorescence
• G11C 13/00 - Digital stores characterised by the use of storage elements not covered by groups , , or

Abstract

The illustrated embodiments include a method of operating a SAW sensor to detect a sample in a fluid which includes the steps of: providing a SAW sensor with a functionalized detection lane in a handheld, portable assay device and sensor system; maintaining the functionalized detection lane of the SAW sensor dry until the sample is fluidically disposed in the detection lane; fluidically disposing the sample in the functionalized detection lane; removing fluid the functionalized detection lane to concentrate the sample in the functionalized detection lane to increase the probability of a specific antibody-antigen interaction; washing the functionalized detection lane so that substantially only the specific antigen-antibody interaction remains in the functionalized detection lane; removing fluid from the functionalized detection lane again; and measuring concentration of the sample while the functionalized detection lane is fluid-free.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 29/02 - Analysing fluids
• G01N 33/536 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase
• A61B 5/318 - Heart-related electrical modalities, e.g. electrocardiography [ECG]

Abstract

A field portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 29/02 - Analysing fluids
• H03H 9/02 - Networks comprising electromechanical or electro-acoustic elementsElectromechanical resonators Details
• H03H 9/145 - Driving means, e.g. electrodes, coils for networks using surface acoustic waves
• G01N 29/036 - Analysing fluids by measuring frequency or resonance of acoustic waves

Abstract

A system and method for performing a portable, fast, field assay of a small sample biological analyte includes a microfluidic cartridge and a reader with which the microfluidic cartridge is selectively communicated. A closed microfluidic circuit mixes and recirculates the analyte with a buffer. A shear horizontal surface acoustic wave (SAW) detector communicates with the microfluidic circuit and has a plurality of channels including at least one functionalized sensing channel in which the mixed analyte and buffer is recirculated and sensed. Capture of the analyte is amplified by recirculation of the analyte and buffer, and detection is amplified by use of an all-purpose endospore display mass amplification.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 29/02 - Analysing fluids
• G01N 29/22 - Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic wavesVisualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object Details

Abstract

A surface acoustic wave (SAW) performs a rapid, label-free detection of biological species. Biosensing and detection of multiple analytes multiplexed by an array of sensing lanes is configured to enable bio-amplification using engineered DNA encoded libraries as the probe through a phage display procedure to enhance specificity, capture statistics for the detection, screening and analyzing of the analyte in vitro. A biochemical formulation minimizes the limit of detection (LOD) at a threshold magnitude on the order of a femtomolar concentration. Additional enhancement of the apparatus is achieved by use of an analog front end to amplify biochemical events.

IPC Classes  ?

• G01N 15/06 - Investigating concentration of particle suspensions
• G01N 29/22 - Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic wavesVisualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object Details
• G01N 29/34 - Generating the ultrasonic, sonic or infrasonic waves
• B81C 1/00 - Manufacture or treatment of devices or systems in or on a substrate
• G01N 29/036 - Analysing fluids by measuring frequency or resonance of acoustic waves
• G01N 29/02 - Analysing fluids
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials

Abstract

An cartridge is combined with a smart device which is capable of communicating with a network to perform a portable, fast, field assay of a small sample biological analyte. A closed microfluidic circuit for mixes the analyte with a buffer with functionalized magnetic beads capable of being specifically combined with the analyte. A detector communicates with the microfluidic circuit in which the mixed analyte, buffer and combined functionalized magnetic beads are sensed. A microcontroller is coupled to detector for controlling the detector and for data processing an output assay signal from the detector. A user interface communicates with the microcontroller for providing user input and for providing user output through the smart device to the network.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 29/02 - Analysing fluids
• G01N 29/036 - Analysing fluids by measuring frequency or resonance of acoustic waves
• H03H 9/145 - Driving means, e.g. electrodes, coils for networks using surface acoustic waves
• G01N 29/24 - Probes
• G01N 29/024 - Analysing fluids by measuring propagation velocity or propagation time of acoustic waves
• G01N 29/22 - Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic wavesVisualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object Details
• H03H 9/05 - Holders or supports
• H03H 9/10 - Mounting in enclosures
• H03H 9/02 - Networks comprising electromechanical or electro-acoustic elementsElectromechanical resonators Details

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

An automated method of assaying a viral and antibody analyte in a sample in a portable, handheld microfluidic reader having a SAW detector with a minimal mass sensitivity limitation includes the steps of automatically performing the assay with the SAW detector with enhanced sensitivity, but also includes the steps of automatically disposing a second portion of the sample on a microarray, selectively automatically probing the second portion of the sample for antibodies corresponding to the at least one selected virus using the microarray, and automatically reading the microarray using a fluorescent camera to identify antibodies in the second portion of the sample.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• G01N 29/02 - Analysing fluids
• G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
• G01N 33/483 - Physical analysis of biological material
• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• G16B 99/00 - Subject matter not provided for in other groups of this subclass

Abstract

Provided herein are methods for preparing a sample comprising a nucleic acid. In some embodiments, the method comprises heating the sample above its boiling point to rapidly extract nucleic acid from biological entities present in the sample.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay