Sugarcane Research Institute Yunnan Academy Of Agricultural Sciences (China)
Inventor
Deng, Jun
Zhang, Yuebin
Yuan, Xin
Mao, Jun
Zhao, Yong
Li, Rudan
Dao, Jingmei
Yang, Shaolin
Wang, Yutong
Ai, Jing
Abstract
A method for tapping a yield potential of Saccharum officinarum by controlling a time of plastic mulching is provided. The method includes: (1) plastic mulching: mulching a Saccharum officinarum furrow in a Saccharum officinarum field fully with a wide plastic film in a direction parallel to the Saccharum officinarum furrow, and covering the plastic film directly above the Saccharum officinarum furrow with soil with a certain thickness along the Saccharum officinarum furrow, such that the plastic film is tightly attached to soil in the Saccharum officinarum furrow; (2) control of the time of plastic mulching, and (3) plastic film recycling treatment. The method of the present disclosure is advanced, practical, easy to operate, and easy to promote.
SUGARCANE RESEARCH INSTITUTE, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Zhang, Yuebin
Deng, Jun
Guo, Jiawen
Li, Rudan
Yang, Shaolin
Dao, Jingmei
Fan, Xian
Abstract
A mechanized planting method for sugarcane in hilly and mountainous areas, comprising the following steps: soil preparation, variety selection, ditching and harvesting. In this way, large-scale, intensive, standardized, mechanized, and simplified production of sugarcane in hilly and mountainous areas has been achieved, labor intensity of sugarcane production has been reduced, labor costs have been saved, and soil fertility and utilization rate have been improved at the same time, promoting the simple and mechanized production of sugarcane in hilly and mountainous areas and increasing the income of sugarcane farmers.
SUGARCANE RESEARCH INSTITUTE, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Mao, Jun
Zhang, Yuebin
Deng, Jun
Dao, Jingmei
Li, Rudan
Abstract
The present invention relates to a sugarcane planting zoning method based on a comprehensive suitability index, comprising the following steps: natural climate condition estimation, soil moisture and nutrient condition estimation, sugarcane production potential estimation, and sugarcane planting zoning based on a comprehensive suitability index. On the basis of deep investigation and estimation on natural environment conditions of a sugarcane zone, the present invention performs classified estimation on climate, soil, and sugarcane production potential elements of a sugarcane planting zone by combining technical methods in meteorology, agrology, sugarcane cultivation science, and crop modeling science, and facilitates rational production layout planning and scientific planting of sugarcane by fully using natural conditions, thereby improving the utilization rate of climate and soil resources, reducing costs and improving effectiveness, and promoting the sugarcane industry to stride forward for an overall target of "high production, high sugar content, high efficiency, intensification, ecology, and safety".
G06Q 10/04 - Forecasting or optimisation specially adapted for administrative or management purposes, e.g. linear programming or "cutting stock problem"
SUGARCANE RESEARCH INSTITUTE, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Zhang, Rong-Yue
Huang, Ying-Kun
Li, Wen-Feng
Wang, Xiao-Yan
Li, Jie
Shan, Hong-Li
Cang, Xiao-Yan
Yin, Jiong
Luo, Zhi-Ming
Abstract
Disclosed are a double PCR detection method for sugarcane white leaf disease phytoplasma and Xanthomonas albilineans and a primer set thereof. The present invention designs a pair of specific primers for the tuf gene of sugarcane white leaf disease phytoplasma and the rpoD gene of Xanthomonas albilineans, respectively, and by means of optimization, establishes a double PCR reaction system that is capable of simultaneously detecting the sugarcane white leaf disease phytoplasma and the Xanthomonas albilineans.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
5.
PCR-RFLP IDENTIFICATION METHOD FOR DIFFERENT SUBGROUPS OF SUGARCANE WHITE LEAF DISEASE PHYTOPLASMA
SUGARCANE RESEARCH INSTITUTE, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Zhang, Rongyue
Huang, Yingkun
Li, Wenfeng
Li, Jie
Shan, Hongli
Wang, Xiaoyan
Cang, Xiaoyan
Luo, Zhiming
Yin, Jiong
Abstract
Disclosed is a PCR-RFLP identification method for different subgroups of sugarcane white leaf disease phytoplasma, wherein a tuf gene of a sugarcane white leaf disease phytoplasma is taken as an object for designing specific primers, a tuf gene fragment is obtained by PCR amplification, and a restriction endonuclease Msp I is then used to digest the tuf gene fragment obtained by amplification, and different subgroups of the sugarcane white leaf disease phytoplasma are distinguished by a differentiated RFLP restriction map. Therefore, a sugarcane white leaf disease phytoplasma subgroup 16SrXI-B and a sugarcane white leaf disease phytoplasma subgroup 16SrXI-D can be identified without sequencing.
C12Q 1/683 - Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
6.
METHOD FOR CONSTRUCTION OF SUGARCANE BROWN RUST RESISTANCE GENE MAPPING GENETIC SEGREGATION COLONY
SUGARCANE RESEARCH INSTITUTE, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Huang, Ying-Kun
Li, Wen-Feng
Wang, Xiao-Yan
Shan, Hong-Li
Zhang, Rong-Yue
Li, Jie
Cang, Xiao-Yan
Yin, Jiong
Luo, Zhi-Ming
Abstract
Disclosed is a method for construction of a sugarcane brown rust resistance gene mapping genetic segregation colony. The method comprises: obtaining F1 generation seeds by means of hybridization between sugarcane varieties of different sources with high susceptibility to brown rust as female parents and sugarcane varieties of different sources with high resistance to brown rust and not containing Bru1 genes as father parents; screening true F1 hybrid seeds by means of seeding; planting true F1 hybrid single plants; inoculating brown rust germs; surveying morbidity; evaluating resistance on the basis of 9-level standards; analyzing the inheritance of disease resistance of true F1 hybrid colonies; selecting a true F1 hybrid colony with a segregation ratio of about 3 to 1 as the sugarcane brown rust resistant gene mapping genetic segregation colony. The method according to the present invention has simplified procedure and good operability, the segregation ratio for the constructed true F1 hybrid colony is about 3 to 1, the disease resistance gene is controlled by dominant monogene and can be used for brown rust resistance gene mapping.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
7.
Method for promoting germination of underground buds and growth of new-born root systems of ratoon sugarcane
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences (China)
Inventor
Deng, Jun
Zhang, Yuebin
Yang, Shaolin
Fan, Xian
Dao, Jingmei
Li, Rudan
Quan, Yiji
Abstract
A method for promoting germination of underground buds and growth of new-born root systems of ratoon sugarcane is to cut stubbles based on a depth survey of the stubbles of the ratoon sugarcane, particularly including steps of: for a ratoon sugarcane field after mechanical harvesting, cutting the stubbles with a sugarcane stubble cutting machine, and remaining underground stubbles of 5-7 cm after cutting; for a ratoon sugarcane field with manual harvesting, cutting the sugarcane with a hoe, and remaining underground stubbles of 10-12 cm after cutting, so as to realize sugarcane cutting and stubble cutting in one step; thereafter, applying fertilizers and pesticides, and hilling up, wherein the underground buds of the stubbles are controlled to be distributed 10-15 cm below ground after hilling up; and finally conducting whole film mulching. The present invention is advanced and practical, and easily used and promoted.
SUGARCANE RESEARCH INSTITUTE OF YUNNAN ACADEMY OF AGRICULTURAL SCIENCES (China)
LINCANG NANHUA SUGAR INDUSTRY CO., LTD. (China)
Inventor
Deng, Jun
Zhang, Yuebin
Dai, Guangwei
Bai, Shengwei
Huang, Pizhong
Fan, Xian
Chen, Guowei
Dao, Jingmei
Li, Rudan
Wu, Jinyu
Abstract
A green, sustainable and high-yield planting method for sugarcane, comprising seven steps, as follows: selecting healthy and high-quality sugarcane seeds, digging furrows for sugarcane, scientifically applying fertilizers, planting seeds and covering with soil, covering with film and covering with soil , harvesting the sugarcane and managing perennial roots. Scientific planting and harvesting are strengthened during two key periods of the sugarcane planting and harvesting. During the period of sugarcane planting, the usage amount of chemical fertilizers is reduced, special slow-release fertilizers for the sugarcane are applied and bio-organic fertilizers are supplied, fully-biodegradable mulching films are used to completely cover, and promote the growth of the sugarcane. Concerning the period of sugarcane harvesting, the traditional harvesting method is altered to cut deep into the soil; fertilizers required by the perennial roots of the sugarcane are then directly applied into the furrows, and the soil is dressed and completely covered with a film, so the maintenance of the perennial roots of the sugarcane can be finished. The method realizes the green production of sugarcane, increasing the yield and the sucrose content, reducing the usage amount of chemical fertilizers, increasing the soil fertility and utilization rate, improving the ecological environment of a sugarcane field, promoting the growth of the sugarcane and increasing the income of sugarcane farmers, and is easily popularized and applied.