The invention relates to a biological indicator for determining the efficacy of a steam or heat sterilization process, and its method. The biological indicator comprises microbial spores (a), at least one sensor protein exogenous to the microbial spores (b), one fluorophore (c), and a culture medium (d). The invention also refers to the method of use of this biological indicator. This method consists of (a) placing the biological indicator along with a target material to be steam or heat sterilized within a steam or heat sterilizer, (b) carrying out a steam or heat sterilization process, (c) placing the biological indicator in a an incubator, (d) screening the biological indicator for immediate detectable changes in fluorescence intensity, while incubating the biological indicator in the incubator, (e) determining the efficacy of the steam or heat sterilization process based on the screening carried out during step d), (f) extending the incubation of the biological indicator obtained in step d), (g) screening the incubated biological indicators obtained in step e) for an optically detectable color change, and (h) determining the efficacy of the steam or heat sterilization process, according to optically detectable changes obtained in step g).
A biological indicator for determining the efficacy of a sterilization process, and its methods of use. The biological indicator comprises a set of microbial spores, at least one sensor molecule, and a culture medium, the sensor molecule being capable of yielding an optically detectable signal when the sensor molecule is not in a inactivated state due to the sterilization process, and a different optically detectable signal when the sensor molecule is in a inactivated state after the sterilization process.
The invention relates to a biological indicator for determining the efficacy of a steam or heat sterilization process, and its method. The biological indicator comprises microbial spores (a), at least one sensor protein exogenous to the microbial spores (b), one fluorophore (c), and a culture medium (d). The invention also refers to the method of use of this biological indicator. This method consists of (a) placing the biological indicator along with a target material to be steam or heat sterilized within a steam or heat sterilizer, (b) carrying out a steam or heat sterilization process, (c) placing the biological indicator in a an incubator, (d) screening the biological indicator for immediate detectable changes in fluorescence intensity, while incubating the biological indicator in the incubator, (e) determining the efficacy of the steam or heat sterilization process based on the screening carried out during step d), (f) extending the incubation of the biological indicator obtained in step d), (g) screening the incubated biological indicators obtained in step e) for an optically detectable color change, and (h) determining the efficacy of the steam or heat sterilization process, according to optically detectable changes obtained in step g).
A biological indicator for determining the efficacy of an oxidative sterilization process, and its methods of use. The biological indicator comprises a set of microbial spores, at least one fluorescent sensor protein, and a culture medium, the fluorescent sensor protein being capable of yielding an optically detectable signal when the fluorescent sensor protein is not in a denatured state due to the oxidative sterilization process, and a different optically detectable signal when the fluorescent sensor protein is in a denatured state after the oxidative sterilization process.
A61L 2/16 - Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lensesAccessories therefor using chemical substances
A61L 2/28 - Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using chemical indicators
5.
BIOLOGICAL INDICATOR FOR DETERMINING THE EFFICACY OF AN OXIDATIVE STERILIZATION PROCESS AND METHODS OF USE
A biological indicator for determining the efficacy of an oxidative sterilization process, and its methods of use. The biological indicator comprises a set of microbial spores, at least one fluorescent sensor protein, and a culture medium, the fluorescent sensor protein being capable of yielding an optically detectable signal when the fluorescent sensor protein is not in a denatured state due to the oxidative sterilization process, and a different optically detectable signal when the fluorescent sensor protein is in a denatured state after the oxidative sterilization process.
C12N 11/00 - Carrier-bound or immobilised enzymesCarrier-bound or immobilised microbial cellsPreparation thereof
A61L 2/28 - Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
G01N 21/77 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
A biological indicator for determining the efficacy of an oxidative sterilization process, and its methods of use. The biological indicator comprises a set of microbial spores, at least one fluorescent sensor protein, and a culture medium, the fluorescent sensor protein being capable of yielding an optically detectable signal when the fluorescent sensor protein is not in a denatured state due to the oxidative sterilization process, and a different optically detectable signal when the fluorescent sensor protein is in a denatured state after the oxidative sterilization process.
A reactive ink composition comprising a UV-C radiation sensitive polymer, an acid scavenger, a photoinitiator, and a pH-sensitive dye. A method to print a composition onto a UV-C radiation dosimeter using a “layer-by-layer” deposition technique, as well as a dosimeter comprising the reactive ink composition, useful in monitoring the efficiency of a UV-C radiation sterilization or disinfection process.
C09D 11/037 - Printing inks characterised by features other than the chemical nature of the binder characterised by the pigment
C09D 11/106 - Printing inks based on artificial resins containing macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds
8.
COMPOSITION SENSITIVE TO UV-C RADIATION AND UV-C STERILIZATION OR DISINFECTION DOSIMETER
A reactive ink composition comprising a UV-C radiation sensitive polymer, an acid scavenger, a photoinitiator, and a pH-sensitive dye. A method to print a composition onto a UV-C radiation dosimeter using a "layer-by-layer" deposition technique, as well as a dosimeter comprising the reactive ink composition, useful in monitoring the efficiency of a UV-C radiation sterilization or disinfection process.
A biological indicator for testing the efficacy of sterilization or disinfection processes of closed environments or confined spaces, the biological indicator comprising a carrier; biological material supported on or embedded in the carrier; and a carrier container comprising a body and a cap. The carrier is fixed within an inner space of the cap, the cap being able to be placed in an open position completely exposing the carrier and the biological material to the closed environment or confined space to be disinfected. A method for determine the efficacy of a process of sterilization or disinfection of closed environments or confined spaces, preferably by means of an enzyme and a fluorescence compound, using said biological indicator.
A biological indicator for determining the efficacy of a steam or heat sterilization process. The biological indicator comprises a set of microbial spores, a sensor protein exogenous to the microbial spores, a fluorophore, and a culture medium. The method of use of this biological indicator for determining the efficacy of a steam or heat sterilization process.
A biological indicator for determining the efficacy of a steam or heat sterilization process. The biological indicator comprises a set of microbial spores, a sensor protein exogenous to the microbial spores, a fluorophore, and a culture medium. The method of use of this biological indicator for determining the efficacy of a steam or heat sterilization process.
The invention relates to a biological indicator for determining the efficacy of a steam or heat sterilization process, and its method. The biological indicator comprises microbial spores (a), at least one sensor protein exogenous to the microbial spores (b), one fluorophore (c), and a culture medium (d). The invention also refers to the method of use of this biological indicator. This method consists of (a) placing the biological indicator along with a target material to be steam or heat sterilized within a steam or heat sterilizer, (b) carrying out a steam or heat sterilization process, (c) placing the biological indicator in a an incubator, (d) screening the biological indicator for immediate detectable changes in fluorescence intensity, while incubating the biological indicator in the incubator, (e) determining the efficacy of the steam or heat sterilization process based on the screening carried out during step d), (f) extending the incubation of the biological indicator obtained in step d), (g) screening the incubated biological indicators obtained in step e) for an optically detectable color change, and (h) determining the efficacy of the steam or heat sterilization process, according to optically detectable changes obtained in step g).
An indicator device and method for monitoring disinfection or sterilization of closed environments and surfaces located in said closed environments, the disinfection and sterilization made by applying a disinfection or sterilization agent, wherein the indicator device comprises a carrier coated or embedded with substances or reagents that produce a change in color or in other readily visible property when contacting said disinfection or sterilization agent, and wherein the carrier is configured as a polyhedron, a cone, a cylinder, a sphere or any three- dimensional shape. A planar carrier having weakened linear areas or groves, printed lines or printed images, and so it is configured to be folded along said linear areas, groves or printed lines or images so as to form a polyhedron, a cone or a cylinder and so it is configured to be folded along said linear areas, groves or printed lines or images so as to form a polyhedron, a cone or a cylinder.
G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using chemical indicators
An indicator device and method for monitoring disinfection or sterilization of closed environments and surfaces located in said closed environments, the disinfection and sterilization made by applying a disinfection or sterilization agent, wherein the indicator device comprises a carrier coated or embedded with substances or reagents that produce a change in color or in other readily visible property when contacting said disinfection or sterilization agent, and wherein the carrier is configured as a polyhedron, a cone, a cylinder, a sphere or any three- dimensional shape. A planar carrier having weakened linear areas or groves, printed lines or printed images, and so it is configured to be folded along said linear areas, groves or printed lines or images so as to form a polyhedron, a cone or a cylinder and so it is configured to be folded along said linear areas, groves or printed lines or images so as to form a polyhedron, a cone or a cylinder.
G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using chemical indicators
15.
Biological disinfection or sterilization indicator
A biological indicator for testing the efficacy of sterilization or disinfection processes of closed environments or confined spaces, the biological indicator comprising a carrier; biological material supported on or embedded in the carrier; and a carrier container comprising a body and a cap. The carrier is fixed within an inner space of the cap, the cap being able to be placed in an open position completely exposing the carrier and the biological material to the closed environment or confined space to be disinfected. A method for determine the efficacy of a process of sterilization or disinfection of closed environments or confined spaces, preferably by means of an enzyme and a fluorescence compound, using said biological indicator.
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