The present invention provides a 5-methylfolate producing microorganism which a) has been modified to have a decreased expression and/or activity of a polypeptide having both dihydrofolate synthase activity and folylpolyglutamate synthetase activity compared to an otherwise identical microorganism (reference microorganism); b) has been (further) modified to express a heterologous polypeptide having only dihydrofolate synthase activity; c) has been (further) modified to have an increased expression level of at least one enzyme (such as at least two, at least three, at least four, at least five, at least six, at least seven or at least eight) enzymes involved in the biosynthesis of a 5-methylfolate compared to an otherwise identical microorganism (reference microorganism); and/or d) has been (further) modified to have a decreased expression and/or activity of a polypeptide having 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity compared to an otherwise identical microorganism (reference microorganism).
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
The present invention generally relates to the biotechnology engineering, and specifically to genetically modified yeast, which allow for the production of vitamin A. More specifically, the present invention provides a genetically modified yeast of the genus Yarrowia capable of producing vitamin A. The present invention also provides methods for the production of vitamin A using a genetically modified yeast of the invention.
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12N 15/52 - Genes encoding for enzymes or proenzymes
Provided is a folate producing strain and the preparation and application thereof, in particular, the expression level of the endogenous folC gene in the engineered strain of the present invention is decreased, and the exogenous folC gene is introduced, and the production capacity of the folate, the precursor, or the intermediate thereof in the engineered strain is significantly improved compared to the starting strain.
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
The present invention provides a 5-methylfolate producing microorganism which a) has been modified to have a decreased expression and/or activity of a polypeptide having both dihydrofolate synthase activity and folylpolyglutamate synthetase activity compared to an otherwise identical microorganism (reference microorganism); b) has been (further) modified to express a heterologous polypeptide having only dihydrofolate synthase activity; c) has been (further) modified to have an increased expression level of at least one enzyme (such as at least two, at least three, at least four, at least five, at least six, at least seven or at least eight) enzymes involved in the biosynthesis of a 5-methylfolate compared to an otherwise identical microorganism (reference microorganism); and/or d) has been (further) modified to have a decreased expression and/or activity of a polypeptide having 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity compared to an otherwise identical microorganism (reference microorganism).
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
C12N 15/52 - Genes encoding for enzymes or proenzymes
5.
FOLATE PRODUCING STRAIN AND THE PREPARATION AND APPLICATION THEREOF
gene in the engineered strain of the present invention is decreased, and the exogenous folC gene is introduced, and the production capacity of the folate, the precursor, or the intermediate thereof in the engineered stain is significantly improved compared to the starting strain.
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
folCfolCfolC gene is introduced, and the production capacity of the folate, the precursor, or the intermediate thereof in the engineered strain is significantly improved compared to the starting strain.
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
Provided is an engineering strain for use in producing β-thymidine, the strain being E. coli BL21, and a deoA gene, a tdk gene and a unp gene in the strain being inactivate or knocked out. Also provided are a method for producing β-thymidine by using the engineered strain and a use for the strain in the fermentation production of the β-thymidine.
Provided are an engineering strain for producing a riboflavin and the use thereof. The strain is an engineering bacterium obtained by gene modification, wherein the gene modification includes the modification of the riboflavin synthesis gene and a promoter thereof.
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