A surface coating method comprises heating a coating solution comprising a gelling agent, at least one sulfate salt and water to a temperature above the melting temperature of the gelling agent but below 100 #C. The heated coating solution is applied onto a plastic surface (12). The coating solution is dried on the plastic surface (12) to obtain a pre-coated plastic surface, onto which a liquid hydrogel is applied. The liquid hydrogel is solidified to form a solid hydrogel (30) adhering to the pre-coated plastic surface. The surface coating (20) enhances the adherence between the solid hydrogel (30) and the plastic surface (12).
Resistance to antibacterial agents and/or specific resistance mechanism of bacteria is predicted by processing, for each antibacterial agent of multiple antibacterial agents, an image set (1) of a plurality of images (2) of a 3D culture matrix (30) comprising the bacteria and over which a concentration gradient of the antibacterial agent is established The processing comprises defining, for each image (2), a plurality of image regions (3) along the concentration gradient, determining, for each image region (3), a parameter (4) representing a quantity of bacteria present in a region of the 3D culture matrix (30) imaged in the image region (3), and generating a data set (5) comprising the parameters (4) determined for the plurality of image regions (3) in the plurality of images (2). Any resistance and/or resistance mechanism is predicated based on the data sets (5) for the multiple antibacterial agents and a resistance and/or resistance mechanism predicting model (125). An early indication of probable antimicrobial resistance and/or presence of specific resistance mechanisms is thereby obtained. (Fig. 7)
A surface coating method comprises heating a coating solution comprising a gelling agent, at least one sulfate salt and water to a temperature above the melting temperature of the gelling agent but below 100C. The heated coating solution is applied onto a plastic surface (12). The coating solution is dried on the plastic surface (12) to obtain a pre-coated plastic surface, onto which a liquid hydrogel is applied. The liquid hydrogel is solidified to form a solid hydrogel (30) adhering to the pre-coated plastic surface. The surface coating (20) enhances the adherence between the solid hydrogel (30) and the plastic surface (12).
B32B 27/08 - Layered products essentially comprising synthetic resin as the main or only constituent of a layer next to another layer of a specific substance of synthetic resin of a different kind
C08J 3/215 - Compounding polymers with additives, e.g. colouring in the presence of a liquid phase the polymer being premixed with a liquid phase at least one additive being also premixed with a liquid phase
C08K 3/30 - Sulfur-, selenium-, or tellurium-containing compounds
A cassette assembly (1) comprises a cover (100), two cassette halves (200A, 200B) and a slider (300) comprising multiple test chambers (302). The cassette halves (200A, 200B) comprise waste tanks (230) in fluid connection with reservoirs (240), prefilled in one of the cassette halves (200A, 200B) with test agents. The particular design of the cassette halves (200A, 200B) enable forming predefined 5 volumes of liquid to achieve predefined concentrations of the test agents in the reservoirs (240) in one of the cassette halves (200A, 200B) and liquid in the reservoirs (240) in the other of the cassette halves (200A, 200B). Gradients of the test agents can then be established over the multiple test chambers (302).
A cassette assembly (1) comprises a cover (100), two cassette halves (200A, 200B) and a slider (300) comprising multiple test chambers (302). The cassette halves (200A, 200B) comprise waste tanks (230) in fluid connection with reservoirs (240), prefilled in one of the cassette halves (200A, 200B) with test agents. The particular design of the cassette halves (200A, 200B) enable forming predefined 5 volumes of liquid to achieve predefined concentrations of the test agents in the reservoirs (240) in one of the cassette halves (200A, 200B) and liquid in the reservoirs (240) in the other of the cassette halves (200A, 200B). Gradients of the test agents can then be established over the multiple test chambers (302).
A fluidic device has a culture chamber configured to house a 3D culture matrix comprising a culture of microorganisms. A concentration gradient of a test substance is established over the 3D culture matrix by providing respective fluid flows at different end portions of the culture chamber and comprising different concentrations of the test substance. The response of the microorganisms to the test substance is determined based on the position of a border zone in the 3D culture matrix.
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
Chemical and biological reagents for medical and veterinary use; diagnostic preparations, substances, test reagents and reagents for medical and veterinary purposes and for medical and veterinary use; medical diagnostic reagents and samples for testing blood Diagnostic apparatus and instruments for analyzing cellular responses for medical and veterinary use; electric instruments for medical and veterinary use for use in the diagnosis of blood
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
Chemical and biological reagents for medical and veterinary use; Diagnostic aids, preparations, substances, test reagents and reagents for medical and veterinary purposes and for medical and veterinary use; Medical diagnostic reagents and samples for testing blood. Diagnostic apparatus and instruments for medical and veterinary use; Electric instruments for medical and veterinary use for use in the diagnosis of blood.
A fluidic device (1) has a culture chamber (10) configured to house a 3D culture matrix (2) comprising a culture of microorganisms (6). A concentration gradient of a test substance is established over the 3D culture matrix (2) by providing respective fluid flows at different end portions (12, 14) of the culture chamber (10) and comprising different concentrations of the test substance. The response of the microorganisms (6) to the test substance is determined based on the position of a border zone (5) in the 3D culture matrix (2).
A fluidic device (1) has a culture chamber (10) configured to house a 3D culture matrix (2) comprising a culture of microorganisms (6). A concentration gradient of a test substance is established over the 3D culture matrix (2) by providing respective fluid flows at different end portions (12, 14) of the culture chamber (10) and comprising different concentrations of the test substance. The response of the microorganisms (6) to the test substance is determined based on the position of a border zone (5) in the 3D culture matrix (2).
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 1/00 - Apparatus for enzymology or microbiology
A microfluidic capsule (1) comprises a top lid (100), a middle piece (200) and a bottom piece (300) to be assembled to enclose a microfluidic substrate (400) for analysis of cells and biochemical reactions. The middle piece (200) comprises support structures in the form of support pillars (250) and walls (240) around a central light window (220) to provide mechanical support and prevent tension-induced structural deformations. When fully assembled, light windows (120, 220, 230) in the top lid (100), middle piece (200) and bottom piece (300) allows inspection of biological and/or biochemical samples positioned in the enclosed microfluidic substrate (400).
A microfluidic capsule (1) comprises a top lid (100), a middle piece (200) and a bottom piece (300) to be assembled to enclose a microfluidic substrate (400) for analysis of cells and biochemical reactions. The middle piece (200) comprises support structures in the form of support pillars (250) and walls (240) around a central light window (220) to provide mechanical support and prevent tension-induced structural deformations. When fully assembled, light windows (120, 220, 230) in the top lid (100), middle piece (200) and bottom piece (300) allows inspection of biological and/or biochemical samples positioned in the enclosed microfluidic substrate (400).
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Chemicals, bio chemicals and reagents used industry and
science; diagnostic preparations, not for medical or
veterinary use; diagnostic assays, not for medical or
veterinary use. Chemical reagents for medical or veterinary use; diagnostic
preparations for medical or veterinary use; diagnostic test
kits for medical or veterinary use. Diagnostic apparatus, not for medical purposes; scientific
and electrical apparatus and instruments for the study and
manipulation of biological cells and compilation of cell
related measuring data.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Chemicals, biochemicals and reagents for industrial and scientific use in microfluidic systems; diagnostic preparations and diagnostic assays for use in microfluidic systems, not for medical or veterinary use Diagnostic, scientific and electrical apparatus and instruments whose functions are based upon fluidic flows in microchannels, for studies and manipulation of biological cells and compilation of cell related measuring data, not for medical use
A fluidic culture device (100) comprises a substrate (101) of transparent polymer material with an open culture chamber (110) in the form of a hollow in a bottom surface (102) of the substrate (101). Open fluid channels (120, 130) flank the culture chamber (110) and have inlets (140, 150) and outlets (160) in a top or end surface (104, 106) of the substrate (101). Removable channel plugs (180, 190) are temporarily arranged in the portions of the fluid channels (120, 130) adjacent the culture chamber (110) to prevent culture matrix material poured into the culture chamber (110) from entering and blocking the fluid channels (120, 130). When assembling an operational culture system (230), a biological sample (210) is introduced in the culture matrix (200) and the channel plugs (180, 190) are removed. A transparent cover disk (220) is reversibly attached to the bottom surface (102) to enclose the culture chamber (110) and the fluid channels (120, 130). A gradient is established over the culture chamber (110) by entering fluids having different concentrations of a substance in the fluid channels (120, 130).
