Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells. Some methods comprise dividing the sample into two or more sub-samples or sample portions, mixing each sub-sample or sample portion with one or more reagents and/or one or more reactants forming distinct sub-sample or sample portion mixtures, compartmentalizing each of the sub-sample or sample portion mixtures into a plurality of small volume compartments, monitoring characteristics of the small volume compartments over time and collecting compartment data, and transmitting the collected data to at least one neural network.
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G16C 20/70 - Machine learning, data mining or chemometrics
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 20/10 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to drugs or medications, e.g. for ensuring correct administration to patients
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
2.
Apparatuses for Contactless Loading and Imaging of Microfluidic Chips and Related Methods
An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
3.
Compositions and methods for disease diagnosis using single cell analysis
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
G01N 15/1433 - Signal processing using image recognition
A method for retaining fluorescent molecules within aqueous droplets suspended in an emulsion includes combining a plurality of living cells, a plurality of fluorescent molecules, and a plurality of cyclodextrin molecules in an aqueous solution. The method also includes emulsifying the aqueous solution with a hydrophobic fluid to form the aqueous droplets. At least one of the aqueous droplets includes (i) a single living cell or a single cell species in a homogenous aggregate of the plurality of living cells, (ii) at least one fluorescent molecule of the plurality of fluorescent molecules, and (iii) at least one cyclodextrin molecule of the plurality of cyclodextrin molecules.
Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.
Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.
A microfluidic chip can include a microfluidic network that comprises a port, one or more test volumes, and one or more channels through which fluid must flow from the port to the test volume(s). A crosslinkable material can also be disposed within the microfluidic network such that the crosslinkable material is flowable through the channel(s). The crosslinkable material of the microfluidic chip may be exposed to light and/or heat to crosslink the material within and thereby occlude the channel(s). A method of loading the microfluidic chip can include disposing a liquid within a port of a microfluidic network that includes one or more test volumes and one or more channels; flowing each of one or more portions of the liquid from the port, through at least one of the channel(s), and into a respective one of the test volume(s); and directing a crosslinkable material into at least one of the channel(s) and cross-linking the crosslinkable material such that none of the test volume(s) are in fluid communication with the port when the portion(s) of the liquid are in the test volume(s).
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
9.
SYSTEMS AND METHODS FOR LOADING REAGENT-CONTAINING MICROFLUIDIC CHIPS HAVING SINGLE-USE VALVES
A microfluidic chip can include a microfluidic network that comprises a port, one or more test volumes, and one or more channels through which fluid must flow from the port to the test volume(s). A crosslinkable material can also be disposed within the microfluidic network such that the crosslinkable material is flowable through the channel(s). The crosslinkable material of the microfluidic chip may be exposed to light and/or heat to crosslink the material within and thereby occlude the channel(s). A method of loading the microfluidic chip can include disposing a liquid within a port of a microfluidic network that includes one or more test volumes and one or more channels; flowing each of one or more portions of the liquid from the port, through at least one of the channel(s), and into a respective one of the test volume(s); and directing a crosslinkable material into at least one of the channel(s) and cross-linking the crosslinkable material such that none of the test volume(s) are in fluid communication with the port when the portion(s) of the liquid are in the test volume(s).
A method for retaining fluorescent molecules within aqueous droplets suspended in an emulsion includes combining a plurality of living cells, a plurality of fluorescent molecules, and a plurality of cyclodextrin molecules in an aqueous solution. The method also includes emulsifying the aqueous solution with a hydrophobic fluid to form the aqueous droplets. At least one of the aqueous droplets includes (i) a single living cell or a single cell species in a homogenous aggregate of the plurality of living cells, (ii) at least one fluorescent molecule of the plurality of fluorescent molecules, and (iii) at least one cyclodextrin molecule of the plurality of cyclodextrin molecules.
A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
12.
SYSTEMS AND METHODS FOR LOADING REAGENT-CONTAINING MICROFLUIDIC CHIPS
A microfluidic device can include a microfluidic circuit that comprises an inlet port, a reagent-containing chamber configured to receive fluid from the inlet port, a non-aqueous-liquid-containing reservoir configured to receive liquid from the chamber, and a droplet-generating region configured to receive and produce droplets of liquid from the reservoir. The circuit can also include first and second valves or frangible members. The first valve or frangible member can have closed position in which fluid is prevented from entering or exiting the chamber therethrough and an open position in which fluid is permitted to enter or exit the chamber therethrough. The second valve or frangible member can have a closed position in which fluid is prevented from flowing between the chamber and the reservoir therethrough and an open position in which fluid is permitted to flow between the chamber and the reservoir therethrough.
A microfluidic device can include a microfluidic circuit that comprises an inlet port, a reagent-containing chamber configured to receive fluid from the inlet port, a non-aqueous-liquid-containing reservoir configured to receive liquid from the chamber, and a droplet-generating region configured to receive and produce droplets of liquid from the reservoir. The circuit can also include first and second valves or frangible members. The first valve or frangible member can have closed position in which fluid is prevented from entering or exiting the chamber therethrough and an open position in which fluid is permitted to enter or exit the chamber therethrough. The second valve or frangible member can have a closed position in which fluid is prevented from flowing between the chamber and the reservoir therethrough and an open position in which fluid is permitted to flow between the chamber and the reservoir therethrough.
Medical apparatus, devices, equipment, and instruments, namely, instruments comprising cameras used to record fluorescence signals and produce data, thermal control elements for heating and cooling, stations for placement of test kits, all for detecting, analyzing, classifying, quantifying, evaluating, monitoring, preparing, testing, mixing, and incubating disease-causing cells and microorganisms for medical, clinical, and diagnostic purposes; medical materials, articles, apparatus, and disposable items, namely, containers to hold samples for medical test purposes, plastic diagnostic test cartridges sold empty, for medical, clinical, and diagnostic use; all of the foregoing only for the purpose of identifying appropriate antibiotics, drugs, and therapies to treat infection or cancer and/or for the treatment of infection or cancer
15.
Compositions and methods for disease diagnosis using single cell analysis
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
A microfluidic chip can comprise a body defining a microfluidic network having one or more inlet ports, a test volume, and one or more flow paths extending between the inlet port(s) and the test volume. Along each of the flow path(s), fluid can flow from one of the inlet port(s), through at least one droplet-generating region in which a minimum cross-sectional area of the flow path increases along the flow path, and to the test volume. The network can include a gutter disposed along at least a portion of the test volume's periphery. The gutter can have a depth along a trough that is at least 10% larger than the depth of the test volume at the periphery and a depth along a ridge disposed between the trough and the test volume that is less than the depth of the test volume at the periphery.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01F 13/00 - Other mixers; Mixing plant, including combinations of dissimilar mixers
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
C12Q 1/08 - Quantitative determination using multifield media
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A microfluidic chip can comprise a body defining a microfluidic network having one or more inlet ports, a test volume, and one or more flow paths extending between the inlet port(s) and the test volume. Along each of the flow path(s), fluid can flow from one of the inlet port(s), through at least one droplet-generating region in which a minimum cross-sectional area of the flow path increases along the flow path, and to the test volume. The network can include a gutter disposed along at least a portion of the test volume's periphery. The gutter can have a depth along a trough that is at least 10% larger than the depth of the test volume at the periphery and a depth along a ridge disposed between the trough and the test volume that is less than the depth of the test volume at the periphery.
An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
19.
APPARATUSES FOR CONTACTLESS LOADING AND IMAGING OF MICROFLUIDIC CHIPS AND RELATED METHODS
An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
20.
Compositions and methods for cellular phenotype assessment of a sample using confined volume arrays
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells. Some methods comprise dividing the sample into two or more sub-samples or sample portions, mixing each sub-sample or sample portion with one or more reagents and/or one or more reactants forming distinct sub-sample or sample portion mixtures, compartmentalizing each of the sub-sample or sample portion mixtures into a plurality of small volume compartments, monitoring characteristics of the small volume compartments over time and collecting compartment data, and transmitting the collected data to at least one neural network.
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G16C 20/70 - Machine learning, data mining or chemometrics
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 20/10 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to drugs or medications, e.g. for ensuring correct administration to patients
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
21.
Apparatuses for contactless loading and imaging of microfluidic chips and related methods
An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
22.
METHODS FOR SCREENING AND SUBSEQUENT PROCESSING OF SAMPLES TAKEN FROM NON-STERILE SITES
A method of analyzing a sample comprising one or more species of microorganisms can include generating first droplets such that each of one or more microorganisms of a first portion of the sample is encapsulated within one of the first droplets and, for each of one or more aliquots of a second portion of the sample, second droplets such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets. First and second sets of data can be captured, the first set indicative of the identity and quantity of encapsulated microorganism(s) of the first portion of the sample and the second set indicative of a phenotypic response of encapsulated microorganism(s) of the aliquot(s) to one or more test reagents. A target species' phenotypic response to the test reagent(s) is determinable at least by referencing the second data set to the first data set.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
H01J 49/16 - Ion sourcesIon guns using surface ionisation, e.g. field-, thermionic- or photo-emission
23.
Methods for Screening and Subsequent Processing of Samples Taken from Non-Sterile Sites
A method of analyzing a sample comprising one or more species of microorganisms can include generating first droplets such that each of one or more microorganisms of a first portion of the sample is encapsulated within one of the first droplets and, for each of one or more aliquots of a second portion of the sample, second droplets such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets. First and second sets of data can be captured, the first set indicative of the identity and quantity of encapsulated microorganism(s) of the first portion of the sample and the second set indicative of a phenotypic response of encapsulated microorganism(s) of the aliquot(s) to one or more test reagents. A target species' phenotypic response to the test reagent(s) is determinable at least by referencing the second data set to the first data set.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
24.
Microfluidic chips including a gutter to facilitate loading thereof and related methods
A microfluidic chip can comprise a body and a microfluidic network defined by the body. The network can include one or more inlet ports, a test volume, and one or more flow paths extending between the inlet port(s) and the test volume. Along each of the flow path(s), fluid is permitted to flow from one of the inlet port(s), through at least one droplet-generating region in which a minimum cross-sectional area of the flow path increases along the flow path, and to the test volume. The network can include a gutter disposed along at least a portion of a periphery of the test volume such that fluid from the flow path(s) is not permitted to flow into the gutter without flowing through the test volume, wherein, along the gutter, a depth of the gutter is at least 10% larger than the depth of the test volume at the periphery.
A microfluidic chip can comprise a body and a microfluidic network defined by the body. The network can include one or more inlet ports, a test volume, and one or more flow paths extending between the inlet port(s) and the test volume. Along each of the flow path(s), fluid is permitted to flow from one of the inlet port(s), through at least one droplet-generating region in which a minimum cross-sectional area of the flow path increases along the flow path, and to the test volume. The network can include a gutter disposed along at least a portion of a periphery of the test volume such that fluid from the flow path(s) is not permitted to flow into the gutter without flowing through the test volume, wherein, along the gutter, a depth of the gutter is at least 10% larger than the depth of the test volume at the periphery.
Disclosed are microfluidic chips and methods of loading the same. Some microfluidic chips include a microfluidic network that has an inlet port, a channel configured to receive liquid from the inlet port, and a droplet-generating region that includes an end of the channel having a transverse dimension, a constant portion extending from the end of the channel and having a constant transverse dimension that is larger than the traverse dimension of the end of the channel, and an expanding portion extending from the constant portion, wherein the transverse dimension of the end of the channel, the transverse dimension of the constant portion, and a length of the constant portion are configured such that, when an aqueous liquid is flowed through the droplet-generating region in the presence of a non-aqueous liquid, droplets of the aqueous liquid are completely formed in the constant portion.
A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
28.
Compositions and methods for disease diagnosis using single cell analysis
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
C12Q 1/18 - Testing for antimicrobial activity of a material
A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.
A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume is described. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.
Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical compositions for use in growing, detecting and/or quantifying cells for scientific and research use; Diagnostic reagents for scientific or research use, namely, reagents used biological samples that have been subdivided into microscopic compartments; Kits comprised primarily of reagents for research purposes in test containers and also including antibiotics in test containers for clinical laboratory use in detecting, quantifying, and analyzing disease-causing micro-organisms; Chemical preparations for scientific purposes and for use in industry other than for medical or veterinary use, namely, reagents, control solutions and control reagents, nutrients, dyes and kits comprised of the foregoing, for scientific, research, industrial, quality control and calibration purposes, and for laboratory and field use in testing the condition of environments, testing food, water, blood, air, and other liquids, powders and substances, for use with scientific and research apparatus, and for use in rapid screening, biological processing and assay analysis; all of the foregoing for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection or cancer and/or for the treatment of infection or cancer and excluding immobilized proteins on a carrier material. Diagnostic reagents, contrast dyes, cell viability dyes and kits comprised of the foregoing, for medical use for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection or cancer and/or for the treatment of infection or cancer; Diagnostic preparations for medical and clinical medical purposes, namely, control solutions, control reagents, diagnostic agents and diagnostic preparations for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection or cancer and/or for the treatment of infection or cancer. Downloadable computer software for use in the healthcare field, namely, software for testing antibiotic resistance, accessing medical reference resources online in aid of providing clinical decision support for antibiotic prescribing, and antibiotic stewardship; software for storing, analyzing, processing, structuring, reviewing, distributing, communicating, organizing, sharing, referencing, monitoring and integrating patient medical information for use in antibiotic prescribing and antibiotic stewardship. Medical apparatus, devices, equipment and instruments, namely, instruments comprising cameras used to record fluorescence signals and produce data, thermal control elements for heating and cooling, stations for placement of test kits, all for detecting, analyzing, classifying, quantifying, evaluating, monitoring, preparing, testing, mixing, and incubating disease-causing cells and microorganisms for medical, clinical and diagnostic purposes; medical materials, articles, apparatus, and disposable items, namely, containers to hold samples for medical test purposes, plastic diagnostic test cartridges sold empty, for medical, clinical, and diagnostic use; all of the foregoing only for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection or cancer and/or for the treatment of infection or cancer. Web-based platform as a service (PAAS) featuring computer software platforms for clinicians and patients to view, store, manage, track, analyze, share and receive patient medical information in the fields of antibiotic prescribing, and antibiotic stewardship.
33.
Compositions and methods for identifying, quantifying, and/or characterizing an analyte
Embodiments of this invention are directed towards the sensitive, fast, and accurate identification and/or characterization of a single cell or bacterium, particularly phenotypic characterization. Certain aspects of the invention include assays that include functional nucleic acid probes (FNAPs). FNAPs can be used to generate deoxyribozyme cleavage cascades (DRCC) initiated by activation of a FNAP resulting in a detectable signal from a single cell.
C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
Chemical compositions for use in growing, detecting and/or quantifying cells for scientific and research use; diagnostic reagents for scientific or research use, namely, reagents used in biological samples that have been subdivided into microscopic compartments; kits comprised primarily of reagents for research purposes in test containers and also including antibiotics in test containers for clinical laboratory use in detecting, quantifying, and analyzing disease-causing micro-organisms; all of the foregoing for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection and excluding immobilized proteins on a carrier material Diagnostic reagents, contrast dyes, cell viability dyes and kits comprised of the foregoing, for medical use for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection and/or for the treatment of infection; diagnostic preparations for medical and clinical medical purposes, namely, control solutions, control reagents, diagnostic agents and diagnostic preparations for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection Medical apparatus, devices, equipment and instruments, namely, instruments comprising cameras used to record fluorescence signals and produce data, thermal control elements for heating and cooling, stations for placement of test kits, all for detecting, analyzing, classifying, quantifying, evaluating, monitoring, preparing, testing, mixing, and incubating disease-causing cells and microorganisms for medical, clinical and diagnostic purposes; medical materials, articles, apparatus, and disposable items, namely, containers to hold samples for medical test purposes, plastic diagnostic test cartridges sold empty, for medical, clinical, and diagnostic use; all of the foregoing only for the purpose of identifying appropriate antibiotics, drugs and therapies to treat infection
35.
Compositions and methods for disease diagnosis using single cell analysis
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
C12Q 1/18 - Testing for antimicrobial activity of a material
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Chemical compositions for use in detecting and/or quantifying biological and chemical molecules. kits comprised of reagents and antibiotics in test containers for clinical laboratory use in detecting, quantifying, and analyzing disease-causing microorganisms. Scientific instruments, namely, bioanalyzers that control, detect, and/or quantify biological and chemical molecules; Instruments and apparatus, namely, microbiology instruments for laboratory use that are comprised primarily of test kits that contain a fluorescence microscope, CCD camera, filters, and an excitation source and are for use in controlling, detecting, and analyzing disease-causing microorganisms.
40.
Compositions and methods for disease diagnosis using single cell analysis
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
C12Q 1/18 - Testing for antimicrobial activity of a material
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
