GE Healthcare Bio-Sciences Corp.

United States of America

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DNA MINI-CIRCLES AND USES THEREOF

      
Document Number 02732212
Status In Force
Filing Date 2009-08-27
Open to Public Date 2010-03-11
Grant Date 2018-05-08
Owner
  • GENERAL ELECTRIC COMPANY (USA)
  • GE HEALTHCARE BIOSCIENCES CORP (USA)
Inventor
  • Nelson, John Richard
  • Wood, Nichole Lea
  • Grossmann, Gregory Andrew
  • Duthie, Robert Scott

Abstract

Methods and kits for generating circular nucleic acids in a cell-free system, and uses for the generated circular nu-cleic acids are provided. The methods comprise in vitro amplification of a nucleic acid template comprising a recombination site to produce tandem repeat nucleic acid sequence, and employ a recombination protein to generate the circular nucleic acids from the tandem repeat nucleic acid sequence.

IPC Classes  ?

  • C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA