Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
B01D 15/10 - Selective adsorption, e.g. chromatography characterised by constructional or operational features
B01D 29/60 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor integrally combined with devices for controlling the filtration
A bioreactor vessel includes a bottom, a peripheral sidewall, the bottom and the peripheral sidewall defining an interior space for receiving a flexible bioprocessing bag, a recess in the bottom for receiving a base plate of the flexible bioprocessing bag, and a locking mechanism configured to retain the base plate in the recess.
Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.
An apparatus for draining a bioreactor vessel includes a tubular body portion having an interior passageway, and at least one aperture in the tubular body portion providing for fluid communication with the interior passageway, the tubular body portion being configured for positioning at a bottom of a vessel, and a suction tube having a first end configured for fluid coupling with the tubular body portion, and a second end configured for fluid coupling with a port in a sidewall of the vessel.
A sparger assembly (800) for a bioprocessing system includes a first layer (806) having a plurality of pores (808) of a first size, and a second layer (810) disposed above the first layer and having a plurality of holes (812) of a second size, the second size being greater than the first size. The pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.
A bioprocessing bag includes a plurality of panels joined to one another about respective edges of the plurality of sheets, by side welds, and a top panel joined to upper edges of the plurality of panels by a flat/convergence weld.
An impeller assembly for a bioprocessing system includes a hub and at least one blade pivotally to the hub, the at least one blade including a first leg portion and a second leg portion extending at an angle from the first leg portion. The at least one blade is rotatable between a first position where the first leg portion extends generally outwardly from the hub and a second position where the second leg portion extends generally outwardly from the hub.
A bioreactor system, includes a base platform, a lid received atop the base platform and defining an interior space for receiving a bioprocessing bag, and a tubing management system supporting a tubing array a distance above the base platform and providing a means for quickly connecting and/or disconnecting a fluid supply line.
A fluid handling apparatus for a bioprocessing system includes a first plate having a first surface and a second surface, at least one fluid flow channel formed in the first surface, at least one valve recess formed in the first surface along the at least one fluid flow channel, and at least one fluid passageway extending through the first plate from the at least one fluid flow channel to the second surface, and a sealing layer disposed over the first surface and enclosing the at least one fluid flow channel. The at least one valve recess is configured to cooperate with an actuator and the sealing layer to prevent a flow of fluid through the at least one fluid flow channel.
A flow distribution device for bioprocess systems, comprising: • - a flow distribution manifold (12; 112; 212; 312 ) comprising: • o at least four fluid connection tubes (14), wherein each fluid connection tube (14) comprises a first end (18) for fluid connection and an opposite second end (20) • o a central common compartment (30; 130; 230; 330) to which the second ends (20) of each of the fluid connection tubes (14) are connected, whereby the first ends (18) of each of the fluid connection tubes (14) can be in fluid communication with the central common compartment (30; 130; 230; 330) • - at least three pinching members (41) which are provided in connection with one fluid connection tube (14) of the flow distribution manifold (12; 112; 212; 312 ) each, wherein each of said pinching members (41) can be controlled into at least a first and a second position, wherein in the first position for each of the pinching members (41) the pinching member pinches one of the fluid connection tubes (14) such that fluid flow is prevented between the first end (18) and the second end (20) of this fluid connection tube (14)
A flow distribution device for bioprocess systems, comprising: a flow distribution manifold (12; 112) comprising: at least four fluid connection conduits (14), wherein each fluid connection conduit (14) comprises a first end (18) for fluid connection and an opposite second end (20), and wherein at least three of the fluid connection conduits comprise a membrane (19a) and a valve seat (19b), which membrane (19a) can be put in at least two different positions in relation to the valve seat (19b) for allowing or preventing fluid flow between the first end (18) and the second end (20) of the fluid connection conduit (14); and a central common compartment (30) to which the second ends (20) of each of the fluid connection conduits (14) are connected, whereby the first ends (18) of each of the fluid connection conduits (14) can be in fluid communication with the central common compartment (30) and wherein the fluid connection conduits (14) are entering the central common compartment (30) from at least three different directions; wherein said flow distribution device (10) further comprises at least three membrane actuation members (41) which are provided in connection with one membrane (19a) of the flow distribution manifold (12; 112) each, wherein each of said membrane actuation members (41) is configured for actuating the membrane (19a) to be in at least two different positions in relation to the valve seat (19b), wherein a first position of the membrane (l9a) allows flow between the first end (18) and the second end (20) of the fluid connection conduit (14) and a second position of the membrane (19a) prevents flow between the first end (18) and the second end (20) of the fluid connection conduit (14).
B01D 15/08 - Selective adsorption, e.g. chromatography
F16K 7/12 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with flat, dished, or bowl-shaped diaphragm
F16K 11/02 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit
F16K 11/22 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit operated by separate actuating members with an actuating member for each valve, e.g. interconnected to form multiple-way valves
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
12.
A SEALING SYSTEM FOR SEALING A TUBE AND AN AUTOMATED METHOD OF OPERATING THE SAME
Disclosed is an automated method (54) which includes directing rays from a source (34) to a tube (38) disposed between relatively movable first and second sealing plates (20,32), capturing an image (70) of at least a portion of the tube (38) by an image capturing device (26), and transferring the captured image (70) to a processing device (24). The method (54) also includes determining a plurality of tube parameters by the processing device (24) based on the captured image (70), using an image processing technique and determining a plurality of sealing parameters from a database (44) by the processing device (24) based on the determined plurality of tube parameters. Additionally, the method (54) includes controlling the drive unit (22) and a heater (36) by the processing device (24) influenced by the determined plurality of sealing parameters, to respectively compress the tube (36)and perform heat sealing of the tube (38).
The present invention relates to a computer implemented method (400) performed by a controller (C) configured to control a bioprocess comprised in a bioreactor (BR), the method comprising obtaining (410) measurement results by performing spectrophotometry of a bioprocessing fluid (FL), generating (420) control parameters based on the measurement results and one model and, controlling (430) the bioprocess using the generated parameters.
A sparger assembly (700) for a bioprocessing system includes a base plate (710) and at least one aeration manifold (712, 714) removably connected to the base plate. Each aeration manifold includes at least one inlet for receiving a gas and a plurality of gas outlet openings for delivering the gas to a fluid within the bioprocessing system. An impeller assembly (740) for a bioprocessing system includes a hub and at least one blade (742) operatively connected to the hub. The at least one blade includes a first portion connected to the hub and extending generally vertically, and a second portion extending at an upward angle from the first portion.
The present disclosure relates to a system and method (10) for monitoring a set-up for manufacture and/or setting up for manufacture and/or tearing down after manufacture of a biopharmaceutical product. The method comprises: processing (S2, S3, S4) at least one image of a scene comprising the set-up for manufacture of the biopharmaceutical product. The processing of the at least one image comprises performing (S2) a first process on the at least one image for classifying first objects in the image, said first objects being devices such as clamps, pumps, valves and/or sensors and/or any other bio processing equipment. The first process comprising identifying, localizing and classifying the first objects in the image. A second process is performed (S3) on the at least one image for identifying and localizing connections in the images. The second process comprises classifying each pixel with an associated second object classifier, said second object classifier classifying a second object selected from a group comprising the first objects and connections, segmenting out pixels associated with a connection and identifying connection mappings with the first objects. The method further comprises forming (S4) compiled information comprising information relating to the identified connection mappings obtained from the second process and the first objects as identified by the first process.
The present disclosure relates to a mixing device (9) comprising an inlet channel (25), an outlet channel (27), a mixing cavity (29) and a laminar mixing element (31) arranged within the mixing cavity. The inlet channel extends along an axis (21) of the mixing device from a first end (23) of the mixing device to the mixing cavity. The outlet channel extends along the axis from the mixing cavity to a second end (24) of the mixing device arranged opposite to the first end. The mixing cavity is arranged between the inlet channel and the outlet channel and extends in an axial direction (A) along the axis and in a radial direction (R) outwardly from the axis to an outer wall (30) of the mixing cavity. The laminar mixing element extends radially across the mixing cavity, subdividing the mixing cavity into an inlet portion (26) and an outlet portion (28). The mixing element comprises a plurality of holes (41) extending through the mixing element. The said holes are distributed over the mixing element.
A method for removing exhaust gas from a bioreactor (3) and a bioreactor system. The method comprises the steps of: - providing at least one exhaust filter (15; 115a, 115b) connected to an outlet (7) of the bioreactor (3) for transferring exhaust gas out from the bioreactor; - increasing a pressure at an inlet side (25) of the at least one exhaust filter (15; 115a, 115b) in a connection (16) between the bioreactor (3) and the at least one exhaust filter (15; 115a, 115b) or decreasing a pressure at an outlet side (23) of the at least one exhaust filter (15; 115a, 115b).
The present invention relates to a bioprocessing fluid sensor arrangement (100) for sensing fluidic properties in a process fluid path with a sensor (S), configured for aseptically connecting the sensor with at least one conditioning fluid while separating said sensor from the process fluid to at least one conditioning fluid e.g. for calibration, cleaning, regenerating and/or storing the sensor, the arrangement comprising a process fluid path (PF) having a process fluid inlet (PI) and a process fluid outlet (PO); a sensor (S) arranged in the process fluid path (PF); a bypass fluid path (BF) in the process fluid path (PF), for bypassing the sensor (S); a conditioning or cleaning fluid path (CF) having an inlet (Cl) and an outlet (CO) each aseptically and fluidically connected to the process fluid path (PF), one on each side of the sensor (S); and flow controls (FC) for controlling the flow of fluids, whereby fluids can be controlled to flow either in the process fluid path (PF) via the sensor (S), or in the bypass fluid path (BF) omitting the sensor from the fluid path (PF), or in the conditioning or cleaning fluid path (CF) including the sensor in said flow but omitting the remaining process fluid path (PF) and bypass fluid path (BF).
A method for fraction collection and a fraction collection system (100) comprising: - a dispensing device (23) including an outlet (24) configured for dispensing fractions of a liquid received from a connected system; - plural receptacles (25) configured for receiving one or more of said fractions, wherein the receptacles (25) and the dispensing device (23) are movable in relation to each other into a number of different positions such that the dispensing device (23) is capable of dispensing a respective fraction of said liquid into one or more of the receptacles (25); - a waste collection device (27), which is arranged in the fraction collection system (100) such that it is movable into at least a first and a second position wherein, in said first position, an inlet (29) of the waste collection device (27) is positioned below the outlet (24) of the dispensing device (23) such that liquid being dispensed from the outlet (24) of the dispensing device (23) is received in the waste collection device (27) and wherein, in the second position, the inlet (29) of the waste collection device (27) is not positioned below the outlet (24) of the dispensing device (23) such that fractions being dispensed from the dispensing device(23) can be received in one or more of the receptacles (25).
B01D 15/24 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
An apparatus for minimizing dead leg spaces in a container or tubing includes a first member having a flange for attaching the first member to a wall of the container or tubing, the flange having at least one aperture, and a second member rotatably coupled to the first member, the second member having an upper end having at least one aperture, and an open distal end. The second member is rotatable relative to the first member between a closed position where the at least one aperture of the second member is misaligned with the at least one aperture in the flange to prevent the passage of fluid, and an open position where the at least one aperture of the second member is aligned with the at least one aperture in the flange to allow for the passage of fluid.
The invention discloses a flexible plastic bag for cultivation of cells, comprising: a top wall film and a bottom wall film, each having an inside and an outside, sealed to each other inside-to-inside by durable weld seams, optionally via one or more side wall films, to form a bag with an inner volume delimited by the durable weld seams; one or more ports through the top and/or bottom wall film for introduction and withdrawal of fluids; one or more frangible weld seams joining the insides of said top and bottom wall films, dividing the bag inner volume into a plurality of cultivation compartments; and one or more gripping means affixed to the top and bottom wall films, adjacent each of the frangible weld seams and adapted to break a specific frangible weld seam by pulling apart the gripping means on the top and bottom wall films adjacent the specific frangible weld seam.
The present disclosure relates to a biological fluid processing system and method. The system comprises a fluid processing device comprising at least one fluid path, a pump for providing a pressure in the at least one fluid path, a valve arranged along said fluid path and a first actuator arranged to control the valve to assume a desired opening state of said fluid path. The biological fluid processing system comprises further a processing interface comprising a pump drive for driving the pump of the fluid processing device, and a processing control element comprising a pump control system arranged to control at least the pump drive and a valve control system arranged to control the first actuator. The system is modular. The fluid processing device is comprised in a fluid processing device module having a predetermined fluid processing device configuration. The processing interfaces have a predetermined processing interface configuration. The processing control element is arranged to receive information relating to the predetermined processing interface configuration of the processing interface module and/or the predetermined fluid processing device configuration of the fluid processing device module and control the at least one pump drive and/or the valve based on the received information relating to the predetermined fluid processing device configuration and the predetermined processing interface configuration.
The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.
B23K 31/12 - Processes relevant to this subclass, specially adapted for particular articles or purposes, but not covered by any single one of main groups relating to investigating the properties, e.g. the weldability, of materials
C12M 1/00 - Apparatus for enzymology or microbiology
G01M 3/28 - Investigating fluid tightness of structures by using fluid or vacuum by measuring rate of loss or gain of fluid, e.g. by pressure-responsive devices, by flow detectors for pipes, cables, or tubesInvestigating fluid tightness of structures by using fluid or vacuum by measuring rate of loss or gain of fluid, e.g. by pressure-responsive devices, by flow detectors for pipe joints or sealsInvestigating fluid tightness of structures by using fluid or vacuum by measuring rate of loss or gain of fluid, e.g. by pressure-responsive devices, by flow detectors for valves
24.
PERFUSION BIOPROCESSING SYSTEM AND METHOD OF OPERATING THE SAME
A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least one first feed flow control device (46) and at least one second feed control device(48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path(14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).
A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device (having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.
The present disclosure relates to aseptic connectors (112) with integrated wireless connectivity including a removable clamp (114) configured to secure a first portion (340) of an aseptic connector to a second portion (320) of the aseptic connector. The removable clamp includes a housing (222), a controller (440) disposed within the housing (222), a rechargeable battery (240) disposed within the housing (222), a wireless radio (260) disposed within the housing (222), and a sensor plug receptacle (250) configured to receive a sensor plug (380) coupled to a sensor (360) of the aseptic connector. The controller (440) is configured to determine a sensor output of the sensor (360) and to transmit the output using the wireless radio (260).
F16L 23/10 - Flanged joints the flanges being connected by members tensioned in the radial plane connection by tangentially arranged pin and nut with a pivoting or swinging pin
27.
SYSTEM FOR FILTRATION AND ASSOCIATED METHOD THEREOF
A method includes inducing a first quantity of a feed fluid (28) to flow along a first direction (72) from a bioreactor (12) to a tangential flow filter (16) to separate the first quantity of the feed fluid (28) into a permeate fluid (42) and a retentate fluid (48). Further, the control unit (66) is operated to control at least one feed flow control device (24, 26) to inhibit the flow of first quantity of the feed fluid (28). Furthermore, the control unit (66) is operated to control the at least one feed flow control device (24, 26) to direct at least one of further flow of a second quantity of the feed fluid (28) from the bioreactor (12), a portion of the permeate fluid (42), and a portion of a nutrient fluid, along a second direction (74) opposite to the first direction (72) via the tangential flow filter (16).
A connection device (1; 101) configured for connecting a separation system (3) with a separation device (5), wherein a fluid can be flowed from a separation system outlet connection (19), through a bypass fluid path (27) of the connection device to the separation system inlet connection (15) while a connected separation device (5) is bypassed. For achieving this at least one of an inlet and an outlet valve (31, 33) provided in the connection device is provided in a closed state and at least one bypass valve (35; 35a, 35b) provided in the connection device is provided in an open state.
The present invention relates to a computer implemented method (600) for optimization of a bioprocessing system (100) formed by interconnected or interconnectable bioprocessing units and configured to provide a desired system functionality, the method comprising obtaining (610) requirement data of a bioprocess, the requirement data being at least indicative of the desired system functionality, and indicative of a desired substance resulting from the operation of the bioprocess system, obtaining (620) unit data indicative of characteristics of a plurality of bioprocessing units, generating (630) at least one optimized or suitable bioprocessing system configuration capable to provide the desired system functionality, the bioprocessing system configuration comprising a selection of bioprocessing units from the plurality of bioprocessing units selected using the requirement data, the unit data and an objective function dependent on the requirement data and the unit data. The invention extends to a configuration system (500).
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
30.
MONITORING SYSTEM AND METHOD FOR BIOPHARMACEUTICAL PRODUCTS
A monitoring system comprises a manufacture system with capability to manufacture a plurality of biopharmaceutical products, at least one image capture device arranged to capture a scene comprising the manufacture system, and a processing element connected to said at least one image capture device and arranged to process images captured by said at least one image capture device to track operator interactions and/or a result of operator interactions within the scene. The processing element is further is arranged to compare a predefined workflow process scheme relating to the selected biopharmaceutical product to at least a part of the tracked operator interactions with the manufacture system and/or to at least a part of the result of the tracked operator interactions with the manufacture system, and determine whether at least one pre-set criterion set by the workflow process scheme is fulfilled based on the comparison.
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
G06Q 10/06 - Resources, workflows, human or project managementEnterprise or organisation planningEnterprise or organisation modelling
A valve (100) for a bioprocess liquid apparatus (199) comprises tubular sections (101, 102, 103) inside which valve seat means (155) are arranged. A plunger arrangement (150) is configured to selectively interact with the valve seat means to close and open the tubular sections in response to a magnetic field provided by solenoid arrangements (104, 105), the solenoid arrangements being arranged at the bioprocess liquid apparatus. A flow path device comprising such a valve and a bioprocess liquid apparatus and a method of handling a bioprocess liquid are also disclosed.
F16K 11/044 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only lift valves with movable valve members positioned between valve seats
F16K 11/056 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only lift valves with ball-shaped valve members
F16K 31/06 - Operating meansReleasing devices electricOperating meansReleasing devices magnetic using a magnet
The present invention relates to a method for optimizing a bioprocess purification system comprising a bioreactor configured to provide a harvest comprising a target composition, and a purification process arranged downstream the bioreactor and being configured for purification of the harvest to produce a target product having a desired characteristics. The method comprising: a) detecting (32) at least one quality attribute indicative of characteristics of the target product in a downstream process, b) identifying (33) correlations between the at least one quality attribute measured in the downstream process and parameters to control a cell culture process in the bioreactor, and c) controlling (34) the cell culture process to meet the desired characteristics based on the identified correlations, whereby the target characteristics is within a p re- determined range.
A method includes measuring, by a flow sensor (70), a flow rate of a permeate fluid (48) flowing through a filter (20) of a membrane filtration system (12). Further, the method includes receiving, by a control unit (86), the measured flow rate of the permeate fluid (48) and determining, by the control unit (86), a first flux rate of the filter (20) based on the measured flow rate of the permeate fluid (48). Furthermore, the method includes comparing, by the control unit (86), the determined first flux rate with a first predetermined flux rate. Additionally, the method includes operating the membrane filtration system (12), by the control unit (86), in a normal mode or a flux tolerant mode based on the comparison of the determined first flux rate with the first predetermined flux rate. The flux tolerant mode of the membrane filtration system (12) is further based on a determined normalized water permeability value of the filter (20).
The present invention relates to a method of automated configuration in a bioprocess system (10)and to verify a process defined by a flow path representation. The method comprises the steps of: -capturing one image of the bioprocess system (10) comprising tubing (50) for the fluid communication between units of the bioprocess system (10); -analyzing the captured image to identify the tubing (50) connecting units of the bioprocess system (10); -producing a processed representation from the captured image wherein at least a part of the tubing (50) is identified;and -comparing the processed representation with the flow path representation to verify their functional concordance.
A bioreactor system includes a vessel having a bottom floor, a flexible bioprocessing bag disposed within the vessel, and a flexible bladder positioned intermediate the bottom floor of the vessel and the bioprocessing bag. The flexible bladder is selectively inflatable to vary at least one of a geometry or configuration of the bioprocessing bag to provide for improved drainability or an increased turndown ratio for the bioreactor system.
Disclosed is an optical flow cell (300') comprising: a housing (910) forming;an enclosed and elongated fluid channel (920) arranged along a first axis (923);a first light guide (961) and a second light guide (962) generally concentrically arranged along a second axis (970) and on opposite side walls of the fluid channel, said first and second light guides having ends (961c,962c) removed in situ to provide a sensing gap (d).
Multicomponent copolymers including two or more types of repeat units is presented. In one example, the multicomponent copolymer includes at least one repeat unit AC having a structure (I), at least one repeat unit DC having a structure (II), and at least one repeat unit BC having a structure (III) or (V). The multicomponent copolymer may be cross-linked via a cross-linking agent. A polymer blend including the multicomponent copolymer or a cross-linked copolymer and a second polymer is also provided. The multicomponent copolymer may be a random or a block copolymer. The structural units of the multicomponent copolymers provide improved, tunable properties, such as improved biocompatibility and hydrophilicity, protein fouling, and mechanical properties, to the copolymers and/or the membranes fabricated from the copolymers.
C08G 65/40 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives derived from phenols from phenols and other compounds
C08G 65/48 - Polymers modified by chemical after-treatment
The invention relates to a a single-use flexible bioreactor bag comprising a rigid multiport plate sealed to a side wall of said bioreactor bag, wherein said multiport plate comprises a plurality of ports. The invention further relates to a method of manufacturing the bag and to a method of installing the bag in a rigid support vessel.
An imaging system 1 is provided with an imaging device 100 and a control device 200 for causing pre-imaging and main imaging to be performed by the imaging device 100. The control device 200 has: an image generation unit 210 that generates a subject image on the basis of one or more captured images in each of the pre-imaging and the main imaging; an interface unit 206 that accepts user input for displaying the subject image and setting a region of interest; a region-setting unit 211 that sets the region of interest on the basis of the user input; an S/N ratio calculation unit 212 that calculates an S/N ratio on the basis of the set region of interest and a region of non-interest; and a total exposure time calculation unit 213 that calculates, on the basis of the S/N ratio calculated for the one or more captured images, a total exposure time by which a maximum S/N ratio or a preset reference S/N ratio is obtained. The control device 200 causes the main imaging to be performed by the imaging device 100 on the basis of the total exposure time.
G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
A multiple cavity reciprocating positive displacement pump (200) comprises a plurality of pump cavities (222), each pump cavity (222) including at least an inlet valve (228) and an outlet valve (231), wherein respective inlet valves (228) are in fluid communication with a common inlet (224) and respective outlet valves (231) are in fluid communication with a common outlet (238), and wherein the pump cavities (222) are arranged about a pump axis (AR) and around said common inlet (224) and said common outlet (238).
F04B 1/047 - Multi-cylinder machines or pumps characterised by number or arrangement of cylinders having cylinders in star- or fan-arrangement with actuating or actuated elements at the outer ends of the cylinders
F04B 43/02 - Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
A method for controlling at least one valve provided to a tube in a bioprocess flow system, said method comprising the steps of: - controlling the at least one valve to be in a first state, which is an open state when a flow through the tube should be allowed; - controlling the at least one valve to be in a second state during some parts of a process run in the system, said second state is a closed state were a first closing force is provided; and - controlling the at least one valve to be in a third state during some parts of a process run in the system, said third state is a closed state were a second closing force is provided which is a higher force than the first closing force.
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16K 7/12 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with flat, dished, or bowl-shaped diaphragm
F16K 31/12 - Operating meansReleasing devices actuated by fluid
F16K 31/126 - Operating meansReleasing devices actuated by fluid the fluid acting on a diaphragm, bellows, or the like
F16K 31/122 - Operating meansReleasing devices actuated by fluid the fluid acting on a piston
42.
APPARATUS, METHOD, AND COMPUTER PROGRAM PRODUCT FOR ADAPTING A PREDEFINED LIQUID CHROMATOGRAPHY PROCESS
The present invention relates to a liquid chromatography system (90) configured to operate with at least one column and configured for purification of a sample comprising a target product using a predefined process. The liquid chromatography system comprises a controller (91) configured to: control the operation of the chromatography system to run the predefined process; retrieve column data accessible from a data storage, the column data being specific to each column; and adapt at least one process parameter of the predefined process for each column based on column data. Whereby the predefined process is adapted to each column to obtain the target product and maintain the performance of the liquid chromatography system.
The present invention relates to a method for purification of viral vectors, more closely it relates to purification of viral vectors from producer cells by using a single automated process. The method comprises the following steps: a) adding producer cells and cell lysis buffer to a processing container; b) mixing said producer cells and cell lysis buffer in said processing container to obtain a mixture; c) flowing said mixture through a chromatography column for purification of viral vectors, wherein the viral vectors are adsorbed on said chromatography column; and d) eluting viral vectors from the chromatography column into a product container.
A probe assembly for inserting a probe into a vessel or tubing includes a probe sheath having a proximal end and a distal end and being configured for operative coupling to a vessel or tubing, the probe sheath being configured to receive a probe and to permit movement of the probe towards the distal end of the probe sheath, and a locking mechanism configured to restrain longitudinal movement of the probe with respect to the sheath in a locked state. The locking mechanism may be unlocked to allow for movement of the probe with respect to the sheath.
G01M 3/00 - Investigating fluid tightness of structures
C12M 1/00 - Apparatus for enzymology or microbiology
G01M 3/32 - Investigating fluid tightness of structures by using fluid or vacuum by measuring rate of loss or gain of fluid, e.g. by pressure-responsive devices, by flow detectors for containers, e.g. radiators
G01G 17/04 - Apparatus for, or methods of, weighing material of special form or property for weighing fluids, e.g. gases, pastes
46.
METHOD AND SYSTEM FOR MANUFACTURING OF BIOPHARMACEUTICAL PRODUCTS
The present disclosure relates to a volume tailorable manufacturing system for quality assured manufacturing of biosafety level classified biopharmaceutical products and a method for tailoring a production volume capability of a manufacturing system. The volume tailorable manufacturing system comprises one or more multi-product suites and a control facility configured to control a unidirectional flow in a circulation system of the one or more multi-product suites. The circulation system is configured to interconnect the one or more multi-product suites and comprises separated supply and return systems. The supply system comprising at least one inlet, the return system comprising at least one outlet that is paired with the inlet and provided at a spatially predetermined position from the inlet, and each inlet/outlet pair comprises a seal when not connected to an adjacent multi-product suite.
The present invention relates to a method for virus assay. More closely the invention relates a method for total quantification of adenovirus in a sample as well as total and functional (active) adenovirus in a sample. The method for determining adenovirus concentration in a sample comprises subjecting said sample to SPR (surface plasmon resonance) assay with immobilized FX (Factor X) and/or immobilized CAR (coxsackievirus and adenovirus receptor) on a sensor surface, wherein the adenovirus concentration is determined from sample binding to immobilized FX and/or immobilized CAR. CAR can be replaced by an ligand binding to adenovirus fiber, such as an anti-adenovirus fiber antibody. FX can be replaced by a ligand binding to adenovirus hexon, such as an anti-adenovirus hexon antibody. The method can be used for quality control in an adenovirus purification process, for example for gene therapy.
A flexible bioprocess bag (1) comprising a number of flexible panels (3a, 3b, 4a, 4b) which are sealed to each other such that when the bag is filled they form at least a bottom (7) of the bag and a side surface (11) of the bag, wherein one of the flexible panels is called a bottom panel (3a) and when the bag is filled said bottom panel (3a) will constitute the bottom (7) of the bag and parts of the side surface (11) of the bag, said parts of the side surface (11) being bent side parts (10a, 10b) of the bottom panel (3a).
A base station (1) and a bioprocess system (3), wherein said base station (1) comprises: - a frame or housing (5), wherein said frame or housing comprises a number of valves (7a-f,9a-d,lla-f,13a-e,15a-f) into which a disposable tubing set (21) during run of the system is provided such that at least one inlet (23a-j) of the disposable tubing set is connected to at least one outlet (25a-f) of the disposable tubing set via a bioprocess separation device (31) of the bioprocess system (3); - a movable pump holding device (35), which can be provided in at least two different positions, whereof one is within the frame or housing (5) and another is at least partly outside the frame or housing (5) and which comprises at least one pump main body (37), said pump main body comprising a connection (39) configured for connecting to a disposable pump connection part (27) comprised in the disposable tubing set (21).
The present invention relates to a valve unit for a chromatography apparatus, the valve unit comprising a fluid inlet configured to receive an input fluid, a fluid outlet configured to provide an output fluid, a first pair of fluid ports configured to be coupled to a first column, a second pair of fluid ports configured to be coupled to a second column, a coupling valve assembly configured to direct fluid between a selection of the fluid inlet, the fluid outlet, the first pair of fluid ports and the second pair of fluid ports in response to one or more control signals, wherein the coupling valve assembly is configured to direct fluid using a selection of membrane valves coupled by fluid channels comprised in a body of the coupling valve assembly. The invention further relates to a chromatography apparatus comprising the valve unit and a membrane valve comprised in the valve unit.
Disclosed is a chromatography system (100) comprising: plural modules (1-25) including at least one pump and a column valve unit (8) connectable to plural chromatography columns; and a controller (600), the controller being operable to control the or each pump and the column valve to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said unit.The system includes a housing (110) into which the plural modules (1-25) are interchangeably mountable in apertures of one generally vertical face of housing, the modules are adapted for selective fluidic interconnection by tubing substantially at said one face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.
in vivoin vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.
The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device 200 configured to remove air from a fluid and comprising the nozzle.
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
B05B 1/04 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops in flat form, e.g. fan-like, sheet-like
B05B 1/08 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops of pulsating nature, e.g. delivering liquid in successive separate quantities
54.
CELL-FREE PROTEIN EXPRESSION USING DOUBLE-STRANDED CONCATAMERIC DNA
vitrovitrovitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences.The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/µLto about 35 ng/µL. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.
The present invention relates to a method for reconstructing events related to a process run in a bioprocess purification system comprising hardware configured to control the events related to the purification of a liquid containing a sample in the bioprocess purification system. The method comprising:recording hardware state S11 related to the process run; recording readings from sensors S12 related to the process run; and synchronizing hardware state S13 with readings from sensors to link the hardware state with the result from the process run. The present invention also related to a method for simulating future events related to a process run in a bioprocess purification system comprising hardware configured to control the events related to the purification of a liquid containing a sample in the bioprocess purification system. The events are controlled by a number of instructions executed consecutively and the method comprising:establishing a current state S21of the process run;assessing an outcome S23 of each non-executed instructions based on information stored in a data storage medium; and predicting future events S24 based on the current state of the process run and the assessed outcome of the non-executed instructions.
A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
Disclosed are chromatography systems employing a releasable coupling (100) for holding a fluid tubing to a spigot, the coupling comprising: a cylindrical inner component (110) for accepting a fluid tubing (30), said inner component including a resiliently deflectable portion (118) arranged to urge an outer surface of the tubing toward a spigot; and a cylindrical collar (130) having and internal through-aperture (132) for slideably accepting the inner component, the aperture and resilient portion having complementary surface formations which in a first position of the collar mounted to the inner component provide for said resilient deflection in use, and which in a second different position do not cause said deflection, the coupling being characterized in that the collar comprises a collar flange (134) extending outwardly away from the aperture of a size allowing manual manipulation of the collar between the first and second positions. Chromatography systems employing said couplings is disclosed also.
F16L 33/22 - Arrangements for connecting hoses to rigid membersRigid hose-connectors, i.e. single members engaging both hoses with means not mentioned in the preceding groups for gripping the hose between inner and outer parts
F16L 37/56 - Couplings of the quick-acting type for double-walled or multi-channel pipes
F16L 37/138 - Couplings of the quick-acting type in which the connection between abutting or axially-overlapping ends is maintained by locking members using hooks, pawls, or other movable or insertable locking members using an axially movable sleeve
58.
METHOD FOR PREDICTING OUTCOME OF AND MODELLING OF A PROCESS IN A BIOREACTOR
The present invention relates to a method for predicting outcome of a process used for manufacturing a sample in a bioreactor, the process belonging to a category. The method comprises selecting (51) a process model based on the category; accessing (53) historic data related to past process runs for manufacturing the sample; accessing (53) current data obtained (54) from a current process run of the process. The obtained current data, which is based on the selected process model, comprises: process strategy data, bioreactor instrument data, data from online sensors and/or data from offline sensors. The method further comprises predicting (62) an outcome of at least one selected parameter of the current process run for manufacturing the sample based on the accessed historic data and current data. The present invention also relates to a method for modelling a process and a control system (10) for controlling a process.
G06F 19/12 - for modelling or simulation in systems biology, e.g. probabilistic or dynamic models, gene-regulatory networks, protein interaction networks or metabolic networks
The present invention relates to a method for production of an improved sensor surface for an SPR instrument, comprising forming a self assembled monolayer (SAM) on a surface and attaching ligands and protein resistant groups, preferably polyethylene glycol (PEG),directly to functional groups on said surface. The invention also relates to a sensor surface produced by these methods use thereof in SPR (surface plasmon resonance) assays or interactions.
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/557 - ImmunoassayBiospecific binding assayMaterials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
60.
METHOD AND APPARATUS FOR DETERMINING ONE OR MORE BUFFER COMPOSITION RECIPES
The present invention relates to an apparatus, a method, a computer program and a computer program product for determining one or more buffer composition recipes. The method comprising obtaining design of experiment, DoE, data, wherein the DoE data is indicative of a set of buffer compositions and corresponding unique recipes, wherein the set of buffer compositions is selected as a subset from a total set of buffer compositions within a design range, running a first set of experiments by consecutively providing buffer compositions mixed according to each unique recipe indicated by the DoE data as the only input to the chromatography apparatus, obtaining results of experiment, RoE, data as output from the chromatography apparatus, wherein the RoE data is indicative of at least a potential of hydrogen, p H, value and a conductivity value of each buffer composition of the set of buffer compositions, obtaining prediction of experimental, PoE data indicative of at least a predicted pH value and a predicted conductivity value of each buffer composition of the total set of buffer compositions, obtaining a first objective function, the first objective function being dependent on a pH value and a conductivity value, selecting a second subset from the total set of buffer compositions which corresponding p H values and conductivity values optimize the first objective function, determining the one or more buffer composition recipes for chromatography of a chemical sample as the unique recipes corresponding to the second subset. The present invention further relates to an apparatus, a computer program and a computer program product.
B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
A method for purification of a target in a solution includes providing a target in a solution, the solution comprising one or more contaminants, providing a polymer conjugate in the solution, wherein the polymer conjugate is configured to specifically bind the target, incubating the solution at a first temperature to facilitate binding of the polymer conjugate to the target, providing an environmentally-responsive block copolymer in the solution, wherein the environmentally-responsive block copolymer comprises one or more of an ethylene oxide (EO), a propylene oxide (PO), or an EO/PO block copolymer, and heating the solution comprising the environmentally-responsive block copolymer and the polymer conjugate bound to the target at a second temperature to initiate a liquid-liquid phase separation, wherein the liquid-liquid phase separation produces an aqueous phase and a liquid polymer phase, and wherein the liquid polymer phase comprises the polymer conjugate bound to the target.
A bio-processing system (100) is presented. The bio-processing system (100) includes one or more bio-processing units (110-114), one or more process supporting devices (116, 118), one or more sensors (120-132), and a bio-processing workflowcontroller (104) wirelessly coupled to at least one of the one or more bio-processing units (110-114), the one or more process supporting devices(116, 118), and the one or more sensors (120-132). The bio- processing workflowcontroller (104)includes a processor (202) configured to create a reconfigurable bio-processing workflow, and where the reconfigurable bio-processing workflow is representative of an arrangement of one or more of the one or more bio-processing units(110-114), the one or more process supporting devices(116, 118), and the one or more sensors (120-32) to perform at least one bio-processing operation.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
63.
SYSTEM AND METHOD FOR ENABLING A SINGLE USE WIRELESS SENSOR BY OPTIMIZING ELECTRICAL POWER
A system for enabling a wireless single use sensing sub-system by optimizing electrical power is presented. The system includes the sensing sub-system configured to be employed in a bio process environment. Further, the sensing sub-system includes a sensing unit configured to measure at least one parameter of the bio process environment. Also, the sensing sub-system includes a power source electrically coupled to the sensing unit and configured to transmit an electrical power to the sensing unit. Furthermore, the sensing sub-system includes a switch configured to electrically couple or decouple the sensing unit from the power source. In addition, the system includes a control sub-system including a first controller configured to determine at least one power control parameter based on a user-input data and asensing sub-system data, and optimize consumption of the electrical power in the sensing sub-system, based on the power control parameter.
H02J 7/02 - Circuit arrangements for charging or depolarising batteries or for supplying loads from batteries for charging batteries from AC mains by converters
H02J 50/80 - Circuit arrangements or systems for wireless supply or distribution of electric power involving the exchange of data, concerning supply or distribution of electric power, between transmitting devices and receiving devices
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
64.
SYSTEM AND METHOD FOR WIRELESSLY POWERING A SENSOR IN A BIO-PROCESSING ENVIRONMENT
A bio-processing system (100) for wirelessly powering one or more sensors (116-128, 400) is presented. The system (100) includes bio-processing units (106-110), process supporting devices (112-114), energy sources (146-148), and sensors (116-128, 400) including an energy harvesting unit (402) and an energy storage unit (404). The system (100) includes a power management subsystem (104, 200) wirelessly coupled to the sensors (116-128, 400) and including a processor (202) configured to wirelessly monitor energy consumption of the sensors (116-128, 400) and a level of energy stored in corresponding energy storage units (404), select at least one sensor (116-128, 400) based on the energy consumption of the sensors (116-128, 400) and corresponding levels of energy stored in the energy storage units (404), and identify at least one active energy source (146-148) as a power source, where the identified power source is configured to wirelessly transfer power to the selected sensor (116-128, 400).
H02J 7/02 - Circuit arrangements for charging or depolarising batteries or for supplying loads from batteries for charging batteries from AC mains by converters
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
H02J 50/80 - Circuit arrangements or systems for wireless supply or distribution of electric power involving the exchange of data, concerning supply or distribution of electric power, between transmitting devices and receiving devices
A connection clamping device (21; 61; 81; 131; 151) arranged for securing a flexible tube (1) to a barbed end (5b) of a tube connector (5), whereby the barbed end (5b) comprises a barb (9) and the tube connector during connection is arranged to protrude into an end of the flexible tube (1), characterized in that said connection clamping device comprises two sections (23 a, 23b) which, during connection when the tube connector protrudes into an end of the flexible tube, are arranged to be connected and locked to each other by a locking mechanism (33a,33b,34a,34b; 63a, 63b, 64a, 64b) around the tube connector (5) and the flexible tube (1), such that the flexible tube (1) is compressed against the tube connector (5) by a rib (41a, 41b) provided on the inner circumference of the connection clamping device.
F16L 33/23 - Arrangements for connecting hoses to rigid membersRigid hose-connectors, i.e. single members engaging both hoses with means not mentioned in the preceding groups for gripping the hose between inner and outer parts the outer parts being segmented, the segments being pressed against the hose by tangentially arranged members
Disclosed is a system for remote controlling and monitoring of at least one instrument, for example a bioprocessing instrument, said system comprising: at least one instrument to be controlled and/or monitored; at least one instrument server connected to the at least one instrument, said instrument server comprising an instrument control software; at least one gateway connected to the at least one instrument server; a transferring means provided in the at least one instrument server, said transferring means being arranged to receive information from the at least one connected instrument and forward said information to the at least one gateway; a first self-hosted web server containing a web application provided in the at least one instrument server for providing possibility to control the at least one instrument via a web browser in an instrument control web page; a publishing means provided in the at least one gateway, said publishing means being arranged to receive information from at least one instrument server and publish said information in an instrument monitoring web page; a second self-hosted web server containing a web application provided in the at least one gateway for providing possibility to monitor the at least one instrument in the instrument monitoring web page via a web browser.
The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.
Disclosed is a modular bio-processing unit (100) comprising: a housing (110) having one or more internal fluid paths (101), the housing having at least one inlet and at least one outlet (5,10,15,20,25,30,35,40,45,50,55,60,65,70), each in fluid communication with the fluid path or one or more of the fluid paths; one or more sensor elements (120,150,170,190) operatively associated with the or each path, said sensor(s) elements including elements of one or more of: a flow sensor, a flow rate sensor, a conductivity sensor, a pressure sensor, a pH sensor, and a light absorbance sensor such as a UV spectroscopic concentration sensor; one or more fluid flow inducing components (140) operatively associated with the or each fluid path; and plural valves (180) for preventing or reducing flow in the or each path, the housing, inlet(s), outlet(s), flow inducing component(s) and valve(s) being arranged to operate together as a bio-processing unit within or substantially within the housing.
The present invention relates to an improved method for transient protein expression which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines expression construct components improving the post-transcriptional processing of a gene of interest, the introduction of a onetime host cell line selection workflow using a template protein of interest expression construct to generate a production competent cell line and approaches enabling inactivation of the template protein of interest. Further expression and protein production of any desired protein of interest using said production competent cell line for transient protein expression processes with improved yields and efficiencies. In a preferred related embodiment of the invention the production competent cell line generated can also be used for CLD using targeted integration and hence the same cell line can be used for production of a desired protein of interest using both transient expression processes and production processes utilizing stable production cells generated using fast CLD workflows.
Systems and methods are provided for performing washing of columns in a simulated moving bed chromatography system while separation is being performed in the system.
The present invention relates to a method for purifying a target product in a flow-through chromatography system comprises at least a first column loaded with feed material from a feed source. The at least first column is purged after binding of impurities and wherein the outlet of purged material from the column is subsequently passed to the feed source.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
The present invention relates to a method for determining operational status of a chromatography column (1; 39, 47, 59; 107, 109, 111, 113), comprising detecting a feed signal (21; 201) representative of the composition of a feed material provided to the inlet of the column; detecting the UV absorbance in the feed material, detecting an effluent signal (23; 203, 205, 207, 209) representative of the composition of the effluent from the column; and using the feed signal and the effluent signal to determine operational status of the column. The feed signal is generated using a first UV detector having a first UV cell pathlength operating at a first UV wavelength and the effluent signal is generated using a second UV detector having a second UV cell pathlength operating at a second UV wavelength. The method further comprising determining a first threshold value based on the detected UV absorbance in the feed material, and selecting the first UV cell pathlength and/or first UV wavelength based on the first threshold value.
Disclosed is a method for automatic installation and setting up of an instrument (1), comprising the steps of: -connecting (S1) the instrument (1) to a service software system (15) in a network (19) via a first communication interface (6); -sending (S3) identification information (5) of the instrument (1) from the instrument to the service software system (15) via the first communication interface (6); -recognizing (S5) in the service software system (15) at least one characteristic of the instrument (1) by analyzing the identification information (5); -based on said at least one characteristic or identification information, creating (S7) by the service software system (15) dedicated high level control software (25) comprising parts of the control software needed for the instrument; -based on said at least one characteristic or identification information, sending (S9) to the instrument (1) from the service software system (15) software to enable a second communication interface (16), and low level control software components needed for local control of the instrument, installing (S11) and configuring in the instrument the low level control software components received from the service software system; -starting (S13) a built-in control software (9) in the instrument; -using the (S15) the built-in control software in the instrument to access the high level control software dedicated for the instrument via the first, or a second communication interface (16), said built-in control software using the low level control software to monitor and control the instrument.
A method and a system and a container system for transferring separation resin from at least one first container (3; 3'; 3a, 3b; 3a', 3b', 3c', 3d') to a second container (5; 5'), wherein said first container is a deformable, single-use separation resin storage container, said method comprising the steps of: - preparing (S1) the at least one first container by providing a deformable, single-use container comprising an outlet port (4') with a predefined volume of separation resin in a storage solution; - fluidizing (S3) the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container; - fluidically connecting (S5) the outlet port (4') of the at least one first container to an inlet port (103) of the second container; - transferring (S7) separation resin from the at least one first container to the second container by generating a pressure difference between an interior of the second container and an interior of the first container where the pressure is lower in the second container.
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
The present invention relates to a method for monitoring operational status in continuous chromatography configured to operate with at least three columns adapted for cyclic use for continuous purification of a target product when feeding the continuous chromatography with a sample containing the target product. The method comprises detecting (81) at least one parameter indicative of a selected function in the continuous chromatography,performing (82) real time trend analysis of the detected parameter to identify a deviating behaviour of the selected function, and initiate (83) actions to eliminate or reduce the effect of the identified deviating behaviour where by the performance of the continuous chromatography is maintained.
The present invention relates to a method for controlling a bioprocess purification system comprising a continuous chromatography configured to operate with at least three columns and configured for cyclic operation, wherein the continuous purification is performed on a sample comprising a target product having desired characteristics. The method comprises detecting (82) at least one parameter indicative of characteristics of the target product, performing (83)real time trend analysis of each detected at least one parameter to identify a deviation from the desired characteristics of the target product, and controlling (84) the bioprocess purification system to meet the desired characteristics based on the identified deviation, whereby the target characteristics is within a pre-determined range.
A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: - providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; - separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; - forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); - eluting the bound biomolecules in the elution cell.
A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.
The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.
A Coriolis flow meter for measuring one or more properties of a fluidis described herein which involves a modular configuration, and includes a fluid flow sub-system and a mechanical oscillator sub-system, both functionally separate, and are coupled in a closed loop arrangement, such that the flow conduit is not directly vibrated, and instead receives induced oscillations from the mechanical oscillator sub-system. The Coriolis flow meter is useful for high purity applications, as well as for the bioprocessing applications. Bioprocessing systems incorporating the Coriolis flow meter are also described herein. Method for measuring one or more properties of a fluid using the disclosed Coriolis flow meter are also described herein.
An impeller (18), for example, a Rushton impeller for a bioreactor system (10) is disclosed. The impeller (18) includes a hub (44), optionaly including a slot (168), a plurality of blades (46), and one or more turbulators (64). The plurality of blades (46) is disposed along a circumferential direction (48) of the hub (44) and spaced apart from each other. Each of the plurality of blades (46) is coupled to at least a portion of a circumference (50) and/or a top surface (1351;1451;1551) of the hub (44). Each blade of the plurality of blades (46) includes a pressure face (52) and a suction face (54). The one or more turbulators (64) is disposed on at least a portion of the suction face (54), the pressure face (52), or both, of a blade of the plurality of blades (46).
The invention relates to an optical flow cell comprising a housing forming an enclosed and elongated fluid channel arranged along a first axis, an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel, a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel. The invention further relates to a corresponding method to produce an optical flow cell.
An optical measuring device (1; 1'; 1''; 41; 61) configured for being connected in a flow path, said optical measuring device (1; 1'; 1''; 41; 61) comprising: -a first and a second flow path connector (5, 7; 5', 7'; 5'', 7''); -at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) provided in between the first and second flow path connectors (5, 7; 5', 7'; 5'', 7'') such that a flow in a flow path in which the optical measuring device (1; 1'; 1''; 41; 61) can be arranged will flow through the first flow path connector (5; 5'; 5''), the at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) and through the second flow path connector (7; 7'; 7''); and -at least one releasable connection device (9) arranged for releasably connecting the at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) in between the first and second flow path connectors(5, 7; 5', 7'; 5'', 7'').
Disclosed is a chromatography column cylinder (200) formed from a polymer composite comprising an outer layer (220) and inner layer (210) intended to be in direct contact with the intended contents of the cylinder in a volume (202), wherein the outer layer supports the inner layer, for example when there is negative pressure in the volume (202). In one embodiment, one of said layers is molded directly onto a surface of the other layer such that there is no gap between the two layers. Disclosed also is a method for forming a chromatography column cylinder.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
A method and a system for transferring a slurry of a separation resin, from at least one first container to a second container, said method comprising the steps of: - connecting the at least one first container to the second container by a transferring connection; - providing a degree of vacuum to the second container by a vacuum production device connected to the second container; - allowing the vacuum in the second container to affect the content in the at least one first container through the transferring connection such that the slurry initially provided in the at least one first container is transferred to the second container through the transferring connection.
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.
A device (60) and method for producing data for correction curves for SPR assays provides a plurality of different concentrations of solvent or other compound to the reference surface and active surface of a SPR chip. The concentrations are mixed online by two pumps (79, 77), one connected to a source (73) of a first concentration of solvent or other compound and the other connected to a source (71) of a second, different concentration of solvent or compound. Mixing of the two different concentrations of solvent or compound can take place in a fluid mixer (75) and the concentration of solvent or compound in the mixture leaving the fluid mixer (75) can be varied by adjusting the relative speeds of the pumps (79, 77). The concentration of the solvent or compound mixture can be adjusted during injection of the mixed liquids in order to provide the different concentrations necessary to make a solvent correction curve.
The present disclosure relates to a manufacturing system, method and control circuitry for quality assured manufacturing of at least two biopharmaceutical products. The manufacturing system (100) comprises a warehouse facility (210), a hydration facility (220), and at least two biopharmaceutical manufacturing facilities, wherein the warehouse facility (210) and hydration facility (220) are comprised in the macro structure (200). Each biopharmaceutical manufacturing facility is comprised in a respective micro node (300), and a control facility (230) in the macro structure (200) is configured to control interoperability of the macro structure (200) and the micro nodes (300) by means of a network spine interconnecting the macro structure (200) with each micro node (300).
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
89.
A METHOD FOR MEASURING THE ABSORBANCE OF A SUBSTANCE IN A SOLUTION AND A MEASURING DEVICE THEREFOR
Disclosed is a method for measuring the absorbance of light of a substance in a solution in a measuring cell (23; 223'), said method comprising the steps of: transmitting (S1) a first light beam (27; 27') from a light source (25; 25') towards a beam splitter (29; 29'); dividing (S3) the first light beam (27; 27') into a signal light ray (31; 31') and a reference light ray (33; 33') by the beam splitter (29; 29'); modulating (S5) the signal light ray (31; 31'); modulating the reference light ray (33; 33'); providing (S9) the measuring cell (23; 23') such that the signal light ray (31; 31') passes through the measuring cell; detecting (S11) a signal in a detectoR (39; 39'), which signal is the combined signal intensity of the signal light ray (31; 31') and the reference light ray (33; 33') detected by the detector (39; 39'); performing synchronous detection (S15) of the detected signal in order to reconstruct the intensities of the signal light ray (31; 31') and the reference light ray (33; 33') from the combined signal detected by the detector (39; 39'), said synchronous detection being based on the modulation performed to the signal light ray and the reference light ray. Disclosed also is a measuring device for carrying out said method
G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Disclosed is a self-adhesive layered septum 10 comprising: a first outer layer 12 comprising a thermoplastic elastomer for example a styrenic block copolymer, containing styrene ethylene butylene styrene ( TPE-SEBS) capable of closing at least partially an aperture formed when a needle is inserted through the layer; an adhesive second layer 22 for adhering the septum to a mouth area of a well or container (17 Figure 2) to which the septum is attachable; and a thermoplastic third layer 32 between the first and second layers, thermobonded to the first layer and providing better adherence for the adhesive layer. The first layer includes a recess 14 and a vent 16, which reduce pressure differentials in use, but together with the third layer minimise evaporation through the septum.
The heating assembly includes a holder having a plurality of segments coupled to each other to define a unitary structure and a heating component coupled to at least one segment of the plurality of segments. The unitary structure includes a top end, a bottom end, and a side wall. The side wall extends between the top end and the bottom end and along a circumferential direction of the heating assembly to define a cavity. At least a portion of the unitary structure has a gradually varied perimeter along a direction perpendicular to a plane which intersects the side wall and is parallel to the top end and the bottom end.
A method for forming polymer microparticles is disclosed. The method comprises spraying a liquid comprising a thermally-gelling polymer from a first orifice of a nozzle into air to form gelled microparticles, and collecting the gelled microparticles with a collecting device. The nozzle is positioned to have a central axis of the first orifice at a tilt angle in a range from about 10 degrees to about 170 degrees relative to a direction of gravity. The collecting device comprises at least two collecting units at different distances from the first orifice of the nozzle in a horizontal direction perpendicular to the direction of gravity. Each of the collecting unit is loaded with a liquid collecting medium. A system for forming polymer microparticles is also disclosed.
The invention discloses a hollow profile wall segment (1) for a bioreactor or mixer support vessel (2;2a;2b), comprising a base profile (3) with two parallel wide sides (4,5), two narrow sides (6,7) and at least one heat exchange channel (8) extending in a longitudinal direction of the base profile through an interior space (9) in the base profile, wherein the hollow profile wall segment is adapted to be joined with similar hollow profile wall segments to form a side wall (12) of a bioreactor or mixer support vessel (2) and wherein each narrow side comprises at least one coupling member (10,11;11a;150) capable of securing the hollow profile wall segment side by side with similar hollow profile wall segments.
Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell- free expression system.
The present invention relates to a method for providing information from a fraction collector in a liquid chromatography system, the system comprising a column, at least one reservoir for containing a fluid, at least one pump connected to said reservoir, a column arranged to receive an input from the reservoir, a measuring device for performing measurements on a fluid output from the column, and a fraction collector for receiving said fluid output in a plurality of receiving vessels, wherein the method comprises - determining at least one parameter of the fraction collector, and - providing information of said parameter on a display in the form of a graphical representation.
Systems and methods for processing biological specimens are provided. The biological specimen processing system generally includes a flow cell carrier for holding a microfluidic flow cell and a fluidic handling unit attachable to the flow cell carrier. The fluidic handling unit interfaces with the microfluidic flow cell and can include fluidic pumps, fluidic connections, integrated electronics, and processing software to facilitate processing of a biological specimen contained in the microfluidic flow cell.
Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.
The present invention provides a method of assaying a sample solution for the presence of a first analyte comprising: (a) providing a sensor surface having a ligand immobilized thereto; (b) flowing the sample solution over the sensor surface; and (c) detecting the presence or absence of binding of the analyte to the ligand on the sensor surface;
wherein the contact time between the sample solution and the immobilized ligand is less than 15 seconds.
The present invention relates to an apparatus for measuring the absorbance of a substance in a solution, comprising at least one sample cell arranged to contain said solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through said at least one sample cell, each of said light passages having a known path length,an LED light source arrangement comprising at least two LEDs, each arranged to emit a light output with a wavelength within said predefined wavelength spectrum,wherein a plurality of optical fibers, one for each light passage, is arranged at each LED for receiving said light output and guiding it to the light passages. The invention also relates to a method for measuring the absorbance of a substance in a solution.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/25 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
100.
METHOD FOR PRESSURE CONTROL IN CROSSFLOW FILTRATION USING HIGH PRECISION PINCH VALVES
The invention discloses embodiments of different high precision pinch control valves having an accuracy in the micron range and a method of controlling a transmembrane pressure (TMP) in a crossflow filtration apparatus, comprising the steps of: a) providing a crossflow filtration apparatus comprising a pump (21) fluidically connected via a retentate compartment (33) of a crossflow filter (32) and a length of flexible tubing to a regulator valve (1;101) acting on the flexible tubing, wherein the regulator valve is a high precision pinch valve. The apparatus further comprises a first pressure transducer (22) adapted to measure a pressure between the retentate compartment and the regulator valve, a second pressure transducer (39) adapted to measure a pressure between the pump and the retentate compartment and a third pressure transducer (40) adapted to measure a pressure at a permeate outlet (36) of the crossflow filter, and a control unit (23) electrically or electromagnetically connected to said regulator valve, the first, second and third pressure transducers and optionally to the pump; b) pumping a liquid with the pump via the second pressure transducer, through the retentate compartment via the length of flexible tubing, the first pressure transducer and the regulator valve; c) controlling the regulator valve with the control unit, such that a TMP calculated by formula (I) TMP = (Pinlet + Poutlet)/2 - Pperm (I)
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
F16K 7/07 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of fluid pressure
F16K 7/16 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with flat, dished, or bowl-shaped diaphragm arranged to be deformed against a flat seat the diaphragm being mechanically actuated, e.g. by screw-spindle or cam
