Abstract

Data storage is provided using double-stranded nucleic acid molecules (70) provided on a thermal control device (52) comprising a plurality of sites (54) and temperature control circuitry (56) to independently control a temperature of each of the plurality of sites. The temperature control circuitry (56), controls the site temperatures to provide a different temperature at a target site compared to other sites of the plurality of sites. The different temperatures at the target site and the other sites provide a greater probability of a read or write operation acting on the target site compared to the other sites. The temperature-based addressing helps to increase physical storage density.

IPC Classes  ?

• G16B 50/20 - Heterogeneous data integration

Abstract

The present invention relates to methods for the high fidelity synthesis of oligonucleotides and polynucleotides on a solid surface. In particular, the invention relates to methods of synthesising oligonucleotides, polynucleotides, and doublestranded polynucleotides/nucleic acids, such as DNA and XNA, wherein the process comprises thermally controlled deprotection steps at the 5'-OH of previously coupled nucleosides or nucleotides at selected sites on the surface of the substrate.

IPC Classes  ?

• C07H 1/00 - Processes for the preparation of sugar derivatives
• C07H 19/073 - Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• C07H 23/00 - Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
• C40B 10/00 - Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins

Abstract

A series of hybridisations is performed for forming a target double-stranded nucleic acid from initial fragments, where each further hybridisation step hybridises the direct products of a pair of earlier hybridisation steps. For at least one further hybridisation step HF, both of the corresponding pair of earlier hybridisation steps HE comprise an error-detecting type of hybridisation step, which includes an error detecting operation to detect whether the hybridised fragments formed in the error-detecting type of hybridisation step HE comprise at least one erroneous hybridised fragment, and discarding at least part of the erroneous fragment to exclude it from a subsequent further hybridisation step. By detecting and removing erroneous fragments throughout a staged and controlled hybridisation process, erroneous fragments are prevented from diluting the pool of error-free fragments at each hybridisation step, to improve yield.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

A temperature control device (2) comprises a number of active thermal sites (6) disposed at respective locations on a substrate (10), each comprising a heating element (13) for applying a variable amount of heat to a corresponding site of a medium and a thermal insulation layer (16) disposed between the heating element and the substrate. At least one passive thermal region (8) is disposed between the active thermal sites (6) on the substrate (10), each passive thermal region (8) comprising a thermal conduction layer (18) for conducting heat from a corresponding portion of the medium to the substrate (10). The thermal conduction layer (18) has a lower thermal resistance in a direction perpendicular to a plane of the substrate (10) than the thermal insulation layer (16). This enables precise control over both heating and cooling of individual sites in a flowing fluid, for example.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• G05D 23/19 - Control of temperature characterised by the use of electric means
• H05B 1/02 - Automatic switching arrangements specially adapted to heating apparatus