The present invention relates to materials and methods for temporary storage of a cereal plant being regenerated, for the production and regeneration of cereal plants, for increasing yield of a regenerated cereal plant, such as wheat plants. In particular, a step of temporarily immersing plantlets in liquid medium at low temperature is provided.
Improved methods and means are provided to modify in a targeted manner the genome of a plant cell at a predefined site using a nucleotide-guided DNA modifying polypeptide such as a RNA-guided endonuclease, a guide- polynucleotide and a donor molecule for repair of the DNA break.
The invention relates to a method for increasing yield in Brassicaceae comprising at least one event or allele as described, wherein the Brassicaceae plants, plant parts thereof, plant propagation material or the soil in which Brassicaceae plants are grown or intended to be grown are treated with active ingredients or mixtures thereof.
The invention relates to a method for increasing yield in Maize plants and plant parts thereof which grow from the seed, by treating the Maize plants and plant parts with active ingredients or mixtures thereof.
The invention relates to a method for increasing yield in wheat or rice plants and plant parts thereof which grow from the seed, by treating the wheat or rice plants and plant parts with active ingredients or mixtures thereof.
The invention relates to a method for increasing yield in Cotton plants and plant parts thereof which grow from the seed, by treating the Cotton plants and plant parts with active ingredients or mixtures thereof.
The invention relates to a method for increasing yield in Soybean plants and plant parts thereof which grow from the seed, by treating the Soybean plants and plant parts with active ingredients or mixtures thereof.
A01N 61/00 - Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
8.
METHOD FOR INCREASING YIELD IN POTATO, TOMATO OR ALFALFA
The invention relates to a method for increasing yield in Potato plants and plant parts thereof which grow from the seed, by treating the Potato plants and plant parts with active ingredients or mixtures thereof; to a method for increasing yield in Tomato plants and plant parts thereof which grow from the seed, by treating the Tomato plants and plant parts with active ingredients or mixtures thereof; and to a method for increasing yield in Medicago spp., preferably M. sativa plants and plant parts thereof which grow from the seed, by treating the Medicago spp., preferably M. sativa plants and plant parts with active ingredients or mixtures thereof.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
C07K 14/325 - Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
The invention relates to a method for increasing yield in Beta spp. plants and plant parts thereof which grow from the seed, by treating the Beta spp. plants and plant parts with active ingredients or mixtures thereof.
The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
The present invention relates to Brassica sequences comprising seed- and funiculus-preferential promoter activity. Provided are recombinant genes comprising the seed- and funiculus-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells, plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression preferentially in the seeds and in the funiculus and to alter biotic or abiotic stress tolerance, yield, seed quality or seed properties.
The present invention relates to Brassica sequences comprising early stage seed-specific and endosperm- preferential promoter activity. Provided are recombinant genes comprising the early stage seed-specific and endosperm-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells, plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the seeds at early developmental stages and preferentially in the endosperm and to alter biotic or abiotic stress tolerance, yield, seed quality or seed properties.
The present invention relates to Brassica sequences comprising early stage seed-specific and endosperm preferential promoter activity. Provided are recombinant genes comprising the early stage seed-specific and endosperm preferential promoter operably linked to a heterologous nucleic acid sequence, and cells, plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the seeds at early developmental stages and preferentially in the endosperm and to alter biotic or abiotic stress tolerance, yield, seed quality or seed properties.
The present invention relates to Brassica sequences comprising late stage seed-specific and embryo-preferential promoter activity. Provided are recombinant genes comprising the late stage seed-specific and embryo-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells, plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the seeds at late developmental stages and preferentially in the embryo and to alter biotic or abiotic stress tolerance, yield, seed quality or seed properties.
The present invention relates to the identification of clubroot resistance genes from Brassica. Clubroot resistant Brassicaceae plants are provided, as well as clubroot resistance genes and methods and means to increase clubroot resistance in Brassicaceae.
The present invention relates to plants having increased number of flowers, pod and increased thousand seed weight (TSW). More specifically, the invention relates to Brassica plants in which expression of Cytokinin oxidase 5 or Cytokinin oxidase 5 and 3 is functionally reduced. Provided are Brassica plants comprising mutant CKX alleles, and Brassica plants in which expression of CKX is reduced. Also provided are methods and means to produce Brassica plants with increased number of flowers, pod or TSW.
The present invention relates to Brassica sequences comprising seed-preferential promoter activity. Provided are recombinant genes comprising the seed-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells, plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the seeds and at a specific seed developmental stage and to alter biotic or abiotic stress tolerance, yield or seed quality.
The present invention relates to a Brassica sequence comprising seed-preferential promoter activity. Provided are recombinant genes comprising the seed-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells,plants and seeds comprising the recombinant gene. The promoter can be used to alter gene expression specifically in the seeds and at a specific seed developmental stage and to alter biotic or abiotic stress tolerance, yield or seed quality.
The present invention relates to Brassica sequences comprising seed-preferential promoter activity. Provided are recombinant genes comprising the seed-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells,plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the seeds and at specific seed developmental stage and to alter biotic or abiotic stress tolerance, yield or seed quality.
The present invention relates to Brassica sequences comprising seed-preferential promoter activity. Provided are recombinant genes comprising the seed-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells,plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the seeds and at a specific seed developmental stage and to alter biotic or abiotic stress tolerance, yield or seed quality.
The present invention relates to the field of agriculture. In particular, the invention provides a promoter, a recombinant gene, plants comprising the recombinant genes and a method to improve yield of a cotton plant under stress conditions.
The present invention relates to Brassica plants comprising mutant FAD2 genes, FAD2 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased levels of C1 8:1 in the seed oil. The invention further relates to combining the mutant FAD2 alleles with mutant FAD3 alleles to increase the levels of C1 8:1 and reduce the levels of C18:3 in the seed oil.
The present invention relates to Brassica plants comprising mutant FAD2 genes, FAD2 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased levels of C18:1 in the seed oil.
The present invention relates to Brassica sequences comprising shoot apex-preferential promoter activity. Provided are recombinant genes comprising the shoot apex -preferential promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the shoot apices and to optimize shoot growth or shoot architecture, improve the resistance to pests and pathogens, improve the tolerance to abiotic stress, confer herbicide resistance, alter the flowering time or improve yield of a plant.
The present invention relates to Brassica sequences comprising leaf-preferential promoter activity, such as leaf-specific or leaf- and cotyledon-specific promoter activity. Provided are recombinant genes comprising the leaf-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the leaves and to alter a plant trait or to produce a commercially relevant product in a plant.
The present invention relates to Brassica sequences comprising root-preferential promoter activity. Provided are recombinant genes comprising the root-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the roots and to alter biotic or abiotic stress tolerance, root architecture, nutrient use efficiency, or yield.
Plantsare providedwith increasedribulose-1,5-bisphosphate (RuBP) regeneration capacity during the Calvin cyclethrough increased expression of seduheptulose 1,7 biosphosphatase, in combination with reduced photo-respiratory losses through expression of glycolate catabolizing enzymes. Such plants have a greater growth rate and/ or improved biomass and/ or increased carbon fixation compared to untreated plants, or plants comprising only one of the features above.
The present application discloses a recombinant fiber-selective promoter region comprising a DNA molecule comprising a fiber specificity region of a cotton lipid transfer protein gene promoter, operably linked to a DNA molecule comprising a nucleotide sequence having at least 90% sequence identity to a nucleotide sequence of about 500 consecutive nucleotides of the 3' end of the FB8-like 2 promoter and use thereof to increase fiber-selective expression of products of interest in cotton fiber cells.
B.G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY (Israel)
Inventor
Barak, Simon
Ransbotyn, Vanessa
Hannah, Matthew
Verduyn, Christoph
Abstract
The present invention concerns a method for increasing stress tolerance and/or biomass comprising modulating the expression of any of the new abiotic stress regulator genes identified herein, as well as chimeric genes, nucleic acids and polypeptides encoding said genes. Also provided are plants or part thereof modulated according to the invention having an increased stress tolerance and/or biomass.
The present invention concerns a method for increasing stress tolerance and/or biomass comprising modulating the expression of any of the genes identified herein, as well as chimeric genes, nucleic acids and polypeptides encoding said genes. Also provided are plants or part thereof modulated according to the invention having an increased stress tolerance and/or biomass.
The present invention concerns a method for increasing stress tolerance and/or biomass comprising modulating the expression of any of the genes identified herein, as well as chimeric genes, nucleic acids and polypeptides encoding said genes. Also provided are plants or part thereof modulated according to the invention having an increased stress tolerance and/or biomass.
The present invention concerns a method for increasing stress tolerance and/or biomass comprising modulating the expression of any of the genes identified herein, as well as chimeric genes, nucleic acids and polypeptides encoding said genes. Also provided are plants or part thereof modulated according to the invention having an increased stress tolerance and/or biomass.
The present invention relates to plants having increased pod drop resistance. More specifically, the invention relates to Brassica plants in which expression of PGAZ is functionally reduced. Provided are Brassica plants comprising mutant PGAZ alleles, and Brassica plants in which expression of PGAZ is reduced. Also provided are methods and means to produce Brassica plants with increased pod drop resistance.
The present invention relates to Brassica sequences comprising pod-preferential promoter activity. Provided are recombinant genes comprising the pod-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the pods and to alter seed yield, seed quality, abiotic stress tolerance, or biotic stress tolerance.
An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase from E. coli which is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.
The present invention relates to an ALS inhibitor herbicide tolerant crop plants, such as allotetraploid Brassica plants, such as B. napus plants, progeny and parts thereof comprising mutations in acetolactase genes.
The present invention relates to Brassica sequences comprising constitutive-preferential promoter activity. Provided are recombinant genes comprising the constitutive promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression in different plant tissues and organ and to alter a plant trait or to produce a commercially relevant product in a plant.
The present invention relates to Brassica sequences comprising endosperm-preferential promoter activity. Provided are recombinant genes comprising the endosperm-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells and plants comprising the recombinant gene. The promoters can be used to alter gene expression specifically in the endosperm and to alter seed properties.
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION (Australia)
Inventor
Meulewaeter, Frank
Llewellyn, Danny J.
Abstract
The present application discloses an isolated nucleic acid sequence comprising (a) at leastabout500 consecutive nucleotides of SEQ ID NO: 1; (b) a nucleotide sequence with at least 95%sequence identity to the nucleotide sequence of (a); (c) a nucleotide sequence hybridizing under stringent conditions to the nucleotide sequence of (a) or (b); and (d) a nucleotide sequence complementary to the nucleotide sequence of any one of (a) to (c). Further disclosed herein is a chimeric gene comprising the isolated nucleic acid described herein operably linked to a nucleic acid coding for an expression product of interest, and a transcription termination and polyadenylation sequence. Also disclosed herein are a vector, a transgenic plant cell, a transgenic plant and a seed as characterized in the claims. Methods disclosed herein relate to the production of a transgenic plant, growing cotton, producing a seed, effecting fiber-preferential expression of a product in cotton and of altering fiber properties in a cotton plant as characterized in the claims.
THE UNIVERSITY COURT OF THE UNIVERSITY OF EDINBURGH (United Kingdom)
Inventor
Meulewaeter, Frank
Van Den Brande, Ilse
Fry, Stephen, C.
Mohler, Kyle, E.
Frankova, Lenka
Simmons, Tom, J.
Holland, Claire
Hudson, Andrew
Abstract
The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activityin addition to mixed-linkage beta-glucan : xyloglucan endotransglucosylase (MXE) activity. The protein may comprisethe amino acid sequence ofany one of SEQ ID NOs: 2, 6 and 8or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving propertiesof cellulosic material.
The present invention relates to the field of plant molecular biology and concerns methods for enhancing photorespiration, photosynthesis, growth or yield in plants by modulating the expression of the glycine decarboxylase, also known as the glycine cleavage system. The present invention also provides recombinant constructs useful in the methods in the invention. In addition, the invention provides transgenic plants having an enhanced photorespiration, photosynthesis, growth or yield.
The present invention relates to an ALS inhibitor herbicide tolerant polyploid plants, such as B. napus or B. juncea plants, progeny and parts thereof comprising mutations in all acetolactase genes.
The present invention relates to an ALS inhibitor herbicide tolerant polyploid plants, such as B. napus or B. juncea plants, progeny and parts thereof comprising a mutation of all acetolactase genes.
The present invention relates to the identification of monocot FLC sequences, such as wheat FLC sequences, as well as their uses in modulating flowering time, seed development and seed germination.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
Methods are provided to increase the level of xyloglucan levels in plant cell walls, including secondary plant cell walls as they can be found in natural fibers or fiber producing plants by expression of a xyloglucan glycotransferase or combined expression of xyloglucan glycotransferase and xyloglucan xylotransferease. The increased xyloglucan level leads to increased capactity for uptake and retention of dyes.
Methods are provided for hybrid seedproduction using 3-phyletic crosses between female, maintainer and male, particularly restorer lines, wherein the trait is introduced in the female line only at the stage of basic seed production via crossing of the female line with a maintainer line containing the gene or genes encoding the trait in homozygous state.
Methods and means are provided to enhance the selective expression of transgenes under control of a fiber-selective promoter, in fiber cells, particularly cotton fiber cells by including target sites for naturally occurring microRNAs with a specific expression profile, particularly with a differential expression profile between cells leading to fibers and other cells of the fiber producing plant, into the transcribed region of genes of interest.
The invention provides methods to determine the ascorbate profile value for plant lines and to use these values in the identification of the better performing plant lines, without having to resort to extensive field trialing.
Provided are transgenic Brassica plants, plant material and seeds, particularly oilseed rape plants, characterized in that these plants harbor a novel combination of two specific transformation events, namely MS-B2 comprising a male-sterility transgene and RF-BN1 comprising a fertility-restoration transgene. Also provided are pairs of Brassica plants comprising each one of the events, and the use thereof in the production of hybrid seed.
Improved methods and means are provided to modify in a targeted manner the genome of a eukaryotic cell at a predefined site using a double stranded break inducing enzyme such as a TALEN and a donor molecule for repair of the double stranded break.
The present invention relates to methods and means to increase seed weight in Brassica. More specifically, the invention relates to mutant DA1 genes in Brassica plants and the use thereof to seed weight.
The present invention relates to methods for separating hybrid seed from a mixture of hybrid seed and inbred seed. In particular, said hybrid seed and said inbred seed have different phenotypes allowing separation of said hybrid seed with non-invasive methods based on the differences in said phenotypes.
The use of compounds of the formulae (I) and (II) for modulating flowering in plants, more preferably for delaying the time to flowering and/or increasing the duration of flowering.
A01N 43/38 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
A01N 47/06 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom containing —O—CO—O— groupsThio-analogues thereof
54.
METHODS, COMPOSITIONS AND DEVICES FOR AMPLIFICATION OF NUCLEIC ACIDS
Methods, kits and compositions of matter are provided which allow amplification of nucleic acid or interest or DNA of interest, comprising the steps of isolating template nucleic acid or DNA from a biological sample using an alkaline extraction solution and directly adding the extract to a reaction mixture, under conditions to amplify the nucleic acids, wherein the isolated template nucleic acid or DNA in the alkaline extraction solution is not diluted or not neutralized prior to the addition to the reaction mixture.
The present invention relates to Brassica juncea ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C1 8: 1 and reduced levels of saturated fatty acids in the seeds.
The present invention relates to soybean ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.
The present invention relates to Brassica plants comprising mutant ROD1 genes, ROD1 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased levels of C18: 1 in the seeds.
Improved methods and means are provided to modify in a targeted manner the genome of a plant cell or plant at a predefined site via bacterial transformation.
The present invention relates to an ALS inhibitor herbicide tolerant B. napus plant, progeny and parts thereof comprising a mutation of an acetolactate synthase I gene and amutation of an acetolactate synthase III gene.
Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to to a field dose of at least 1X of at least one HPPD inhibitor, and methods for making such plants.
In one aspect, the application discloses a cotton plant cell comprising (a) a chimeric gene comprising a first nucleic acid sequence comprising at least 700 consecutive nucleotides of SEQ ID NO: 1 or a nucleic acid sequence having at least 80% sequence identity thereto any of which has stomata-preferential promoter activity; (b) a second nucleic acid sequence encoding an expression product of interest; and (c) a transcription termination and polyadenylation sequence. In addition, the present application discloses a cotton plant, a method of expressing a transgene in cotton under stress conditions, a method of producing a cotton plant, a method of detecting the expression of a transgene under stress conditions and a method for modulating the resistance of a cotton plant to stress as characterized in the claims.
Methods and means are provided to modify in a targeted manner the genome of a cotton plant using a double stranded DNA break inducing enzyme and embryogenic callus.
The present application discloses a(n) (isolated) nucleic acid sequence comprising a nucleotide sequence selected from (a) SEQ ID NO: 1 or a fragment thereof, wherein said fragment comprises at least 400 consecutive nucleotides of SEQ ID NO: 1 and has seed-specific promoter activity; (b) a nucleotide sequence with at least 80% sequence identity with SEQ ID NO: 1 and having seed-specific promoter activity; (c) a nucleotide sequence hybridizing under stringent conditions to the nucleotide sequence of (a) or (b); and (d) a nucleotide sequence complementary to the nucleotide sequence of any one of (a) to (c). Further disclosed herein is a chimeric gene comprising the (isolated) nucleic acid described herein operably linked to a nucleic acid coding for an expression product of interest, and optionally a transcription termination and polyadenylation sequence. Also disclosed herein are a vector, a transgenic plant cell, a transgenic plant and a seed as characterized in the claims. Methods disclosed herein relate to the production of a transgenic plant, growing cotton, producing a seed, effecting seed-specific expression of a product in cotton and of altering fiber properties in a cotton plant as characterized in the claims.
In one aspect, the present application discloses a chimeric gene comprising (a) a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80% sequence identity thereto any of which confers stress inducibility on said chimeric gene; (b) a second nucleic acid sequence encoding an expression product of interest, which is involved in the response of a cotton plant to stress; and optionally (c) a transcription termination and polyadenylation sequence. In another aspect, the application discloses a cotton plant cell comprising (a) a chimeric gene comprising a first nucleic acid sequence comprising at least 400 consecutive nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 or a nucleic acid sequence having at least 80 sequence identity thereto any of which confers stress inducibility on said chimeric gene; (b) a second nucleic acid sequence encoding an expression product of interest; and optionally (c) a transcription termination and polyadenylation sequence. In addition, the present application discloses a cotton plant, a method of expressing a transgene in cotton under stress conditions, a method of producing a cotton plant, a method of detecting the expression of a transgene under stress conditions and a method for modulating the resistance of a cotton plant to stress as characterized in the claims.
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANIZATION (Australia)
Inventor
Meulewaeter, Frank
Llewellyn, Danny
Abstract
The present invention relates to materials and methods for the expression of a gene of interest selectively in cotton fibers. In particular, the invention provides an expression cassette for regulating fiber-selective expression in plants.
The present invention relates to Brassica plants comprising mutant ALC genes, ALC nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased podshatter resistance.
Methods and means are provided to produce positively charged oligosaccharides in the plant cell wall by introducing into said plant cell a Nodulation C protein fused to a heterologous Golgi signal anchor sequence.
Means and methods are provided to produce abiotic stress tolerant with improved yield based on the specific identification of a gene expression signature in said plants out of a population of said plants.
Methods and means to alter the glucosinolate (GSL) content in plants, in particular in specific plant parts, by modifying glucosinolate transporter protein (GTR) activity in plants or parts thereof are herein described. In particular, methods are provided to decrease GSL content of plant seed and meal thereof, as well as methods to increase GSL content in green plant tissue, of Brassicales plants.
Means and methods are provided to produce abiotic stress tolerant plants with improved yield based on the specific identification of a DNA methylation signature in said plants out of a population of said plants.
Methods and means are provided to modify in a targeted manner the plant genome of transgenic plants comprising chimeric genes wherein the chimeric genes have a DNA element commonly used in plant molecular biology. Re-designed meganucleases to cleave such an element commonly used in plant molecular biology are provided.
Methods and means are provided to modify in a targeted manner the plant genome of transgenic plants comprising chimeric genes wherein the chimeric genes have a DNA element commonly used in plant molecular biology. Re-designed meganucleases to cleave such an element commonly used in plant molecular biology are provided.
The present invention relates to Brassica plants comprising at least one mutant dwarfing allele of a DELLA protein encoding gene, nucleic acid sequences representing mutant DELLA dwarfing alleles, and mutant dwarfing DELLA proteins. The invention further relates to methods for generating and identifying said plants and alleles, which can be used to obtain plants with reduced height and increased lodging resistance.
Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants.
The invention provides specific transgenic rice plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the rice genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
The present invention relates to Brassica plants comprising full knockout AHAS alleles and to brassica plant comprising a combination of full knockout AHAS alleles and AHAS alleles encoding herbicide tolerant AHAS proteins, nucleic acid sequences representing full knockout AHAS alleles, as well as methods for generating and identifying said plants and alleles, which can be used to obtain herbicide tolerant plants.
The present invention relates to Brassica plants comprising mutant FAD3 alleles, FAD3 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to obtain seed oil with a reduced alpha-linolenic acid content.
The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
79.
HERBICIDE TOLERANT SOYBEAN PLANTS AND METHODS FOR IDENTIFYING SAME
The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a stack of specific transformation events at specific locations in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of these events in biological samples.
Methods and means are provided to alter lipid biosynthesis in eukaryotic organisms by targeting at least two different polypeptides involved in fatty acid or lipid metabolism towards a similar or the same subdomain of an organelle, such as the endoplasmatic reticulum (ER), through fusion of the polypeptides with a similar or the same heterologous polypeptide targeting the chimeric fusion polypeptide to the mentioned subdomain.
Bacillus thuringiensis, and their use in plants to control insect pests. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising a Cry1 C chimeric gene of the invention and at least one other chimeric gene, such as a chimeric gene encoding an insecticidal Cry1Ab protein, and methods of making or using such plant cells or plants.
The invention provides specific transgenic rice plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the rice genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
patents
